CN217948150U

CN217948150U - Stem cell culture perfusion device

Info

Publication number: CN217948150U
Application number: CN202221872724.1U
Authority: CN
Inventors: 牛丹
Original assignee: First Affiliated Hospital of Medical College of Xian Jiaotong University
Current assignee: First Affiliated Hospital of Medical College of Xian Jiaotong University
Priority date: 2022-07-18
Filing date: 2022-07-18
Publication date: 2022-12-02
Anticipated expiration: 2032-07-18

Abstract

The utility model discloses a stem cell culture perfusion device, the on-line screen storage device comprises a base, base upper end fixedly connected with framework, fixedly connected with fixed plate between a plurality of inner walls of framework, seted up a plurality of through holes on the fixed plate, it is a plurality of all be equipped with the blake bottle in the through hole, logical groove has all been seted up on the lateral wall of framework both sides, two sliding connection has the movable plate between the logical groove, the movable plate lower extreme is seted up flutedly, be equipped with the perfusion mouth that a plurality of equidistant settings in the recess, it is a plurality of all be equipped with the solenoid valve in the perfusion mouth, the movable plate upper end is equipped with and is used for carrying out the feeding mechanism of feed to a plurality of perfusion mouths, be equipped with on the lateral wall on the framework right side and be used for carrying out the moving mechanism that removes to the movable plate. The utility model discloses a make the movable plate remove, make a plurality of perfusion mouths of movable plate lower extreme carry out the perfusion to a plurality of blake bottles in the framework, when improving perfusion efficiency, avoid the condition that the nutrient solution revealed to appear among the perfusion in-process.

Description

Stem cell culture perfusion device

Technical Field

The utility model relates to a stem cell culture technical field especially relates to a stem cell culture perfusion device.

Background

Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, are capable of producing at least one type of highly differentiated progeny cells, and have been subject to revision over the years and definition at different levels. Most biologists and physicians currently believe that stem cells are a class of cells derived from embryonic, fetal, or adult human bodies that have the ability to renew and proliferate and differentiate without restriction under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, and also capable of producing specialized cells that make up tissues and organs of the body, and also capable of differentiating into progenitor cells.

Present stem cell tissue liquid all needs the nutrient solution to cultivate when detecting with other medical treatment, and the stem cell test tube of a batch needs quantitative nutrient solution to cultivate, and the perfusion to stem cell nutrient solution among the prior art is generally all to carry out the perfusion to a plurality of blake bottles through manual in proper order, the condition that the nutrient solution was revealed easily appears in the perfusion in-process, work efficiency is also comparatively low simultaneously, and present sealing to the blake bottle generally only goes on through the sealing plug, sealed effect is comparatively abundant, and still need open it one by one when the perfusion, the operation is complicated, the linkage of reducing means.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the shortcoming that exists among the prior art, and the stem cell culture perfusion device that proposes, through making the movable plate remove, make a plurality of perfusion mouths of movable plate lower extreme carry out the perfusion to a plurality of blake bottles in the framework, when improving perfusion efficiency, avoid the perfusion in-process to appear the condition that the nutrient solution was revealed, and a stem cell culture perfusion device that proposes.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a stem cell culture perfusion device, includes the base, base upper end fixedly connected with framework, the fixedly connected with fixed plate between a plurality of inner walls of framework, a plurality of through holes have been seted up on the fixed plate, and are a plurality of all be equipped with the blake bottle in the through hole, logical groove has all been seted up on the lateral wall of framework both sides, two sliding connection has the movable plate between leading to the groove, the movable plate lower extreme is seted up flutedly, be equipped with a plurality of equidistant irritated mouth that set up in the recess, it is a plurality of all be equipped with the solenoid valve in the irrigated mouth, the movable plate upper end is equipped with the feed mechanism that is used for carrying out the feed to a plurality of irritated mouths, be equipped with the moving mechanism that is used for removing the movable plate on the lateral wall of framework right side.

Preferably, the feeding mechanism comprises a conveying pipe arranged at the upper end of the moving plate, a corrugated pipe is sleeved on the outer wall of the conveying pipe, and the conveying pipe is communicated with the plurality of perfusion openings.

Preferably, moving mechanism includes the connecting plate of fixed connection on the lateral wall of movable plate right side, be equipped with the telescopic cylinder of two symmetry settings on the lateral wall of framework right side, two telescopic cylinder's flexible end all sets up on the lateral wall of connecting plate.

Preferably, the base upper end is equipped with the nutrition box, the nutrition box upper end is equipped with the water pump, the conveyer pipe is kept away from the one end setting of movable plate at the output of water pump.

Preferably, a limiting groove is formed in the inner wall of the left side of the frame body, a sealing plate penetrates through the limiting groove and the side wall of the right side of the frame body in a sliding connection mode, a limiting plate is arranged between the side wall of the right side of the sealing plate and the connecting plate, and the sealing plate is connected with the moving plate through a connecting block.

Preferably, the connecting plate is L-shaped, and the connecting plate is made of high-strength stainless steel.

The utility model has the advantages that:

1. through making the movable plate remove, make a plurality of perfusion mouths of movable plate lower extreme carry out the perfusion to a plurality of culture bottles in the framework, when improving perfusion efficiency, avoid the condition that the nutrient solution revealed to appear in the perfusion in-process.

2. Under the cooperation through closing plate and spacing groove, seal a plurality of blake bottles, guarantee the leakproofness of blake bottle, the removal of rethread movable plate drives the closing plate and removes, improves the device linkage.

Drawings

FIG. 1 is a schematic front view of the overall structure of the present invention;

FIG. 2 is a schematic bottom view of the overall structure of the present invention;

FIG. 3 is a schematic cross-sectional view of the internal structure of the frame body of the present invention;

fig. 4 is an enlarged schematic view of a structure shown in fig. 3 according to the present invention.

In the figure: the device comprises a base 1, a nutrient box 2, a water pump 3, a delivery pipe 4, a corrugated pipe 5, a frame 6, a movable plate 7, a connecting plate 8, a limiting plate 9, a telescopic cylinder 10, a groove 11, a perfusion opening 12, a fixing plate 13, a culture bottle 14, a sealing plate 15, a limiting groove 16 and a connecting block 17.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-4, a stem cell culture perfusion device, including a base 1, a fixedly connected frame 6 is arranged on the upper end of the base 1, a fixed plate 13 is fixedly connected between a plurality of inner walls of the frame 6, a plurality of through holes are formed in the fixed plate 13, culture bottles 14 are arranged in the through holes, through grooves are formed in the side walls of two sides of the frame 6, a movable plate 7 is slidably connected between the two through grooves, a groove 11 is formed in the lower end of the movable plate 7, a plurality of perfusion ports 12 which are arranged at equal intervals are formed in the groove 11, electromagnetic valves are arranged in the perfusion ports 12, a feeding mechanism for feeding the perfusion ports 12 is arranged on the upper end of the movable plate 7, the feeding mechanism comprises a conveying pipe 4 arranged on the upper end of the movable plate 7, a corrugated pipe 5 is sleeved on the outer wall of the conveying pipe 4, and the conveying pipe 4 is communicated with the perfusion ports 12.

Be equipped with on the lateral wall of framework 6 right side and be used for carrying out the moving mechanism that removes to movable plate 7, moving mechanism includes connecting plate 8 of fixed connection on the lateral wall of movable plate 7 right side, the shape of connecting plate 8 is L shape, the material of connecting plate 8 is the high strength stainless steel, be equipped with the telescopic cylinder 10 that two symmetries set up on the lateral wall of framework 6 right side, the flexible end of two telescopic cylinder 10 all sets up on the lateral wall of connecting plate 8, through making movable plate 7 remove, make a plurality of perfusion mouths 12 of movable plate 7 lower extreme carry out the perfusion to a plurality of culture bottles 14 in the framework 6, when improving perfusion efficiency, avoid the perfusion in-process the condition that the nutrient solution was revealed to appear.

Base 1 upper end is equipped with nutrition box 2, and 2 upper ends of nutrition box are equipped with water pump 3, and the one end setting that the movable plate 7 was kept away from to conveyer pipe 4 is at the output of water pump 3.

The limiting groove 16 is formed in the inner wall of the left side of the frame body 6, the sealing plate 15 penetrates through the limiting groove 16 and the side wall of the right side of the frame body 6, the limiting plate 9 is arranged between the side wall of the right side of the sealing plate 15 and the connecting plate 8, the sealing plate 15 and the movable plate 7 are connected through the connecting block 17, the plurality of culture bottles 14 are sealed through the matching of the sealing plate 15 and the limiting groove 16, the sealing performance of the culture bottles 14 is guaranteed, the sealing plate 15 is driven to move through the movement of the movable plate 7, and the linkage performance of the device is improved.

When the utility model is used, when nutrient perfusion is needed to be carried out on a plurality of culture bottles 14 on a fixed plate 13 in a frame body 6, only two telescopic cylinders 10 are needed to be started, the telescopic ends of the two telescopic cylinders 10 push a connecting plate 8 to move, the connecting plate 8 pulls a moving plate 7 to move in a through groove, a plurality of perfusion ports 12 in a groove 11 at the lower end of the moving plate 7 move, under the connecting action of a connecting block 17 between the moving plate 7 and a sealing plate 15 and the connecting action of a limiting plate 9 and the connecting plate 8, the sealing plate 15 moves along with the movement of the moving plate 7, so that the sealing plate 15 relieves the sealing of the plurality of culture bottles 14, when the plurality of perfusion ports 12 move to the position right above the culture bottles 14, the electromagnetic valves in the multiple perfusion ports 12 are controlled to be opened through the prior art (the opening and closing of the control electromagnetic valves are the existing mature technology, so that related structures of the electromagnetic valves are not described in the drawing), meanwhile, the water pump 3 is started to pump out nutrient solution in the nutrient box 2 through the water pump 3 and then the nutrient solution is conveyed to the multiple perfusion ports 12 through the conveying pipe 4 to flow out, the culture bottles 14 are perfused, after perfusion operation of the multiple culture bottles 14 is completed, the telescopic ends of the two telescopic cylinders 10 can drive the connecting plate 8 and the moving plate 7 to return to the initial position, meanwhile, one end of the sealing plate 15 can also stretch into the limiting groove 16 to seal the multiple culture bottles 14, the corrugated pipe 5 is arranged, the conveying pipe 4 can change the length according to the movement of the moving plate 7, and the adaptability of the device is improved.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a stem cell culture perfusion device, includes base (1), its characterized in that, base (1) upper end fixedly connected with framework (6), fixedly connected with fixed plate (13) between a plurality of inner walls of framework (6), a plurality of through holes have been seted up on fixed plate (13), and is a plurality of all be equipped with blake bottle (14) in the through hole, logical groove has all been seted up on framework (6) both sides lateral wall, two sliding connection has movable plate (7) between the logical groove, recess (11) have been seted up to movable plate (7) lower extreme, be equipped with a plurality of equidistant perfusion mouth (12) that set up in recess (11), it is a plurality of all be equipped with the solenoid valve in perfusion mouth (12), movable plate (7) upper end is equipped with the feed mechanism that is used for carrying out the feed to a plurality of perfusion mouth (12), be equipped with the moving mechanism that is used for moving movable plate (7) on framework (6) right side lateral wall.

2. Stem cell culture perfusion apparatus according to claim 1, wherein the feeding mechanism comprises a conveying pipe (4) disposed at an upper end of the moving plate (7), the outer wall of the conveying pipe (4) is sleeved with a corrugated pipe (5), and the conveying pipe (4) is communicated with the plurality of perfusion ports (12).

3. A stem cell culture perfusion device according to claim 2, wherein the moving mechanism comprises a connecting plate (8) fixedly connected to a right side wall of the moving plate (7), two symmetrically arranged telescopic cylinders (10) are arranged on the right side wall of the frame body (6), and telescopic ends of the two telescopic cylinders (10) are both arranged on a side wall of the connecting plate (8).

4. A stem cell culture perfusion device according to claim 3, wherein a nutrient tank (2) is arranged at the upper end of the base (1), a water pump (3) is arranged at the upper end of the nutrient tank (2), and one end of the conveying pipe (4) far away from the movable plate (7) is arranged at the output end of the water pump (3).

5. A stem cell culture and perfusion device according to claim 4, wherein a limiting groove (16) is formed in the inner wall of the left side of the frame body (6), a sealing plate (15) is connected between the limiting groove (16) and the side wall of the right side of the frame body (6) in a penetrating and sliding manner, a limiting plate (9) is arranged between the side wall of the right side of the sealing plate (15) and the connecting plate (8), and the sealing plate (15) is connected with the moving plate (7) through a connecting block (17).

6. A stem cell culture perfusion device according to claim 5, wherein the connecting plate (8) is L-shaped, and the connecting plate (8) is made of high-strength stainless steel.