CN214244419U - Adjustable oxygen concentration blake bottle - Google Patents
Adjustable oxygen concentration blake bottle Download PDFInfo
- Publication number
- CN214244419U CN214244419U CN202023094113.2U CN202023094113U CN214244419U CN 214244419 U CN214244419 U CN 214244419U CN 202023094113 U CN202023094113 U CN 202023094113U CN 214244419 U CN214244419 U CN 214244419U
- Authority
- CN
- China
- Prior art keywords
- air inlet
- air outlet
- oxygen concentration
- air
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 239000001301 oxygen Substances 0.000 title claims abstract description 41
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 41
- 238000007789 sealing Methods 0.000 claims abstract description 21
- 230000000149 penetrating effect Effects 0.000 claims abstract description 3
- 239000007789 gas Substances 0.000 claims description 16
- 241001411320 Eriogonum inflatum Species 0.000 claims description 7
- 210000004027 cell Anatomy 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an adjustable oxygen concentration culture bottle, which relates to the technical field of culture bottles and comprises a bottle body, a bottle mouth arranged at the top of the bottle body and a bottle plug filled with the inner wall of the bottle mouth, wherein an air inlet channel and an air outlet channel are arranged inside the bottle plug, an air inlet is arranged at the top of the air inlet channel, the air inlet penetrates through the top of the bottle plug, the top of the air inlet channel is communicated with an air inlet pipe, an air inlet sealing block, a first spring, a fixed block and an air inlet disc are arranged inside the air inlet channel from top to bottom, the air inlet sealing block, the first spring, the fixed block and the air inlet disc are fixedly connected one by one, an air outlet disc, a fixed block, a second spring and an air outlet sealing block are arranged inside the air outlet channel from top to bottom, an exhaust pipe is arranged at the top of the air outlet sealing block, an exhaust port is arranged at the bottom of the exhaust port penetrating through the bottom of the bottle plug, the adjustable oxygen concentration culture bottle adopts an inlet channel and an air outlet channel, the oxygen concentration in the culture bottle can be adjusted.
Description
Technical Field
The utility model relates to a blake bottle technical field specifically is an adjustable oxygen concentration blake bottle.
Background
In vitro culture of cells and tissues has become an essential element in life research and practice. The cell types in culture are numerous, from viruses to bacteria and fungi, from human cells to animal and plant cells. Culture flasks (also referred to as "cell culture flasks") are used in experiments to culture or harvest cells, tissues or microorganisms, cell culture is an important experiment in biological, medical research, some cells and tissues can grow in suspension, but a significant proportion of mammalian cells require surface adherence. In recent years, with the continuous development of biotechnology, cell culture technology is changing day by day, and in order to better meet the demand of cell culture, various culture bottles appear, but the demand of different microorganisms on oxygen concentration is different, and at present, most of culture bottles in laboratories cannot regulate the oxygen concentration in the culture bottles at any time, so that the culture effect is poor, and even cells and the like in the culture bottles die.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model provides an adjustable oxygen concentration blake bottle compares with prior art, the regulation that carries out oxygen concentration that can be better more convenient.
In order to achieve the above object, the utility model provides a following technical scheme: an oxygen concentration adjustable culture bottle, which comprises a bottle body, a bottle mouth arranged at the top of the bottle body, and a bottle plug inserted into the inner wall of the bottle mouth, an air inlet channel and an air outlet channel are arranged in the bottle stopper, an air inlet is arranged at the top of the air inlet channel, the air inlet penetrates through the top of the bottle stopper, the top of the air inlet is communicated with an air inlet pipe, an air inlet sealing block, a first spring, a fixing block and an air inlet disc are arranged in the air inlet channel from top to bottom, the air inlet sealing blocks, the first springs, the fixed blocks and the air inlet discs are fixedly connected one by one, the air outlet discs, the fixed blocks, the second springs and the air outlet sealing blocks are arranged in the air outlet channel from top to bottom, the air outlet disc, the fixing block, the second spring and the air outlet sealing blocks are fixedly connected one by one, an exhaust pipe is arranged at the top of the air outlet disc, an exhaust port is arranged at the bottom of the air outlet sealing blocks, and the exhaust port penetrates through the bottom of the bottle stopper.
Preferably, an intake valve is provided in the middle of the intake pipe.
Preferably, an exhaust valve is arranged in the middle of the exhaust pipe.
Preferably, a gas filter is arranged at the joint of the gas inlet pipe and the gas inlet.
Preferably, the top of the air inlet pipe is connected to an external feeding device.
Preferably, through holes are formed in the air inlet disc and the air outlet disc in a penetrating mode.
Compared with the prior art and products, the beneficial effects of the utility model are that:
this kind of adjustable oxygen concentration blake bottle, adopt and be equipped with into passageway and air outlet channel, realize the adjustable of the oxygen concentration in the blake bottle, through the air inlet, admit air the seal block, first spring, fixed block and parts such as the disk of admitting air, when oxygen concentration reduces, the pressure differential of formation, the air inlet has been opened, let in oxygen, thereby oxygen concentration has been improved, through the gas vent, the disk of giving vent to anger, the fixed block, second spring and parts such as the seal block of giving vent to anger, when oxygen concentration risees, form the pressure differential with the external world, the gas vent has been opened, exhaust gas, thereby oxygen concentration has been reduced, more convenient oxygen concentration regulation has been realized.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a cross-sectional view of the bottle stopper of the present invention;
fig. 3 is a schematic view of the structure at the through hole of the present invention.
In the figure: 1. a bottle body; 2. a bottle mouth; 3. a bottle stopper; 4. an air inlet pipe; 5. an intake valve; 6. a gas filter; 7. an air inlet sealing block; 8. an air inlet; 9. a first spring; 10. a fixed block; 11. an air intake disc; 12. an air intake passage; 13. an air outlet channel; 14. an exhaust port; 15. an air outlet sealing block; 16. a second spring; 17. an air outlet disc; 18. an exhaust valve; 19. an exhaust pipe; 20. and a through hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Please refer to fig. 1-3: the utility model provides an adjustable oxygen concentration blake bottle, includes bottleneck 2 that body 1 and body 1 top were equipped with to and the bottle plug 3 that 2 inner walls of bottleneck were filled in, inlet channel 12 and outlet channel 13 have been seted up to the inside of bottle plug 3.
Specifically, as shown in fig. 2, the top of air inlet channel 12 is equipped with air inlet 8, air inlet 8 runs through the top of bottle plug 3, the top intercommunication of air inlet 8 has intake pipe 4, the centre of intake pipe 4 is equipped with admission valve 5, the inside from the top down of air inlet channel 12 is equipped with air inlet sealing block 7, first spring 9, fixed block 10 and air inlet disc 11, air inlet sealing block 7, first spring 9, fixed block 10 and air inlet disc 11 fixed connection one by one, air inlet disc 11's inside runs through and is equipped with a plurality of through-hole 20.
Further, the top of the air inlet pipe 4 is connected to an external oxygen supply device, and when the oxygen concentration needs to be increased, the external oxygen supply device supplies oxygen required by the culture bottle.
Further, intake pipe 4 is equipped with gas filter 6 with 8 junctions of air inlet, and by the oxygen that intake pipe 4 got into, some impurity are filtered to the oxygen that enters into gas filter 6 for oxygen concentration obtains further improvement, thereby can make oxygen concentration obtain improving fast in the blake bottle.
Specifically, when the inside oxygen concentration of blake bottle reached suitable, first spring 9 just balanced, at this moment, the block 7 that admits air just seals air inlet 8 completely, when oxygen concentration reduces in the blake bottle, inside atmospheric pressure also can reduce, thereby form the pressure differential with the outside, make the block 7 that admits air move down, first spring 9 compresses, open admission valve 5, make oxygen pass through inlet channel 12 by intake pipe 4, get into the blake bottle by through-hole 20, make inside oxygen concentration obtain increasing, state before first spring 9 resumes, the block 7 that admits air seals air inlet 8.
Specifically, as shown in fig. 2, an air outlet disc 17, a fixed block 10, a second spring 16 and an air outlet sealing block 15 are arranged in the air outlet channel 13 from top to bottom, the air outlet disc 17, the fixed block 10, the second spring 16 and the air outlet sealing block 15 are fixedly connected one by one, an exhaust pipe 19 is arranged at the top of the air outlet disc 17, an exhaust valve 18 is arranged in the middle of the exhaust pipe 19, an exhaust port 14 is arranged at the bottom of the air outlet sealing block 15, the exhaust port 14 penetrates through the bottom of the bottle stopper 3, and a plurality of through holes 20 are formed in the air outlet disc 17.
Specifically, when the inside oxygen concentration of blake bottle reaches suitable, second spring 16 just balances, at this moment, give vent to anger and seal piece 15 and just seal gas vent 14 completely, when oxygen concentration increases in the blake bottle, inside atmospheric pressure also can increase, thereby form the pressure differential with the outside, give vent to anger and seal piece 15 and move up, second spring 16 compression, thereby the inside gas of blake bottle has through-hole 20 and gets into air outlet channel, open discharge valve 18, gas can be discharged by blast pipe 19, thereby can reduce the concentration of inside oxygen, state before second spring 16 resumes, give vent to anger and seal piece 15 and seal gas vent 14.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (6)
1. The utility model provides an adjustable oxygen concentration blake bottle, includes bottleneck (2) that body (1) and body (1) top were equipped with to and bottle plug (3) that bottleneck (2) inner wall was filled in, its characterized in that: air inlet channel (12) and air outlet channel (13) have been seted up to the inside of bottle plug (3), the top of air inlet channel (12) is equipped with air inlet (8), air inlet (8) run through the top of bottle plug (3), the top intercommunication of air inlet (8) has intake pipe (4), the inside from the top down of air inlet channel (12) is equipped with admits air a sealing piece (7), first spring (9), fixed block (10) and air inlet disc (11), admit air a sealing piece (7), first spring (9), fixed block (10) and air inlet disc (11) one by one fixed connection, the inside from the top down of air outlet channel (13) is equipped with air outlet disc (17), fixed block (10), second spring (16) and air outlet sealing piece (15), air outlet disc (17), fixed block (10), second spring (16) and air outlet sealing piece (15) one by one fixed connection, the top of the air outlet disc (17) is provided with an air outlet pipe (19), the bottom of the air outlet sealing block (15) is provided with an air outlet (14), and the air outlet (14) penetrates through the bottom of the bottle stopper (3).
2. The adjustable oxygen concentration culture flask according to claim 1, wherein: and an air inlet valve (5) is arranged in the middle of the air inlet pipe (4).
3. The adjustable oxygen concentration culture flask according to claim 1, wherein: an exhaust valve (18) is arranged in the middle of the exhaust pipe (19).
4. The adjustable oxygen concentration culture flask according to claim 1, wherein: and a gas filter (6) is arranged at the joint of the gas inlet pipe (4) and the gas inlet (8).
5. The adjustable oxygen concentration culture flask according to claim 1, wherein: the top of the air inlet pipe (4) is connected with an external feeding device.
6. The adjustable oxygen concentration culture flask according to claim 1, wherein: and through holes (20) are formed in the air inlet disc (11) and the air outlet disc (17) in a penetrating manner.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202023094113.2U CN214244419U (en) | 2020-12-21 | 2020-12-21 | Adjustable oxygen concentration blake bottle |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202023094113.2U CN214244419U (en) | 2020-12-21 | 2020-12-21 | Adjustable oxygen concentration blake bottle |
Publications (1)
Publication Number | Publication Date |
---|---|
CN214244419U true CN214244419U (en) | 2021-09-21 |
Family
ID=77740336
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202023094113.2U Active CN214244419U (en) | 2020-12-21 | 2020-12-21 | Adjustable oxygen concentration blake bottle |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN214244419U (en) |
-
2020
- 2020-12-21 CN CN202023094113.2U patent/CN214244419U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN201626959U (en) | Micro perfusion device for cell culture | |
JP2018529367A (en) | Bioreactor capable of shutting off gas supply | |
CN201442951U (en) | Airlift type photo-bioreactor | |
CN205398612U (en) | Improve photobioreactor of little algae growth rate | |
CN101613664A (en) | A kind of airlift photobioreactor | |
CN214244419U (en) | Adjustable oxygen concentration blake bottle | |
CN105602847A (en) | Centrifugal settling-type bioreactor for continuous culture of plant cells | |
Wakisaka et al. | Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484 | |
CN207468646U (en) | High efficiency cell culture bottle | |
CN205501326U (en) | Centrifugal sedimentation formula bioreactor for plant cell continuous culture | |
CN203057904U (en) | Simple fermentation device for liquid strain submerged cultivation | |
CN201389209Y (en) | Ultraviolet sterilization system of air needed by culture environment of needle mushroom liquid spawn | |
CN204151338U (en) | A kind of fermentation of Aspergillus niger nutrient solution sampler | |
CN210314291U (en) | Shake flask for active air exchange for culturing bacteria/cells | |
CN206457493U (en) | A kind of pocket type anoxic box | |
CN217868918U (en) | Cell culture dish | |
CN2151154Y (en) | Cycling cell cultivator | |
CN1293799C (en) | Method and apparatus for producing and inoculating liquid baterial spawn in suspension mode | |
CN215856095U (en) | Chlorella is bred and uses harvesting device | |
CN218290912U (en) | Constant-temperature strain culture oxygenation shake flask | |
CN217895599U (en) | Can airtight large capacity cell culture bottle | |
CN218089551U (en) | OA aerobic device | |
CN209685787U (en) | One Yeasts culture bottle | |
CN214244420U (en) | Simple industrial microorganism activation culture device and system | |
CN201092575Y (en) | Divided cell culture bag |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: Warehouse 1A, No. 115 Nanxiang Road, Hongqi Town, Jinwan District, Zhuhai City, Guangdong Province, 519000 Patentee after: Zhuhai Andier Biotechnology Co.,Ltd. Country or region after: China Address before: 519000, 1st Floor, Building 15, No. 3 Nanping Pingdong Sixth Road, Xiangzhou District, Zhuhai City, Guangdong Province Patentee before: Zhuhai Andier plastic products Co. Country or region before: China |