CN105219616A - The board-like bioreactor of a kind of software - Google Patents
The board-like bioreactor of a kind of software Download PDFInfo
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- CN105219616A CN105219616A CN201410270445.1A CN201410270445A CN105219616A CN 105219616 A CN105219616 A CN 105219616A CN 201410270445 A CN201410270445 A CN 201410270445A CN 105219616 A CN105219616 A CN 105219616A
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Abstract
The present invention relates to microdisk electrode photobioreactor field.The board-like bioreactor of software of the present invention, comprise runner, also comprise bioreactor culture liquid entrance (1), the outlet of bioreactor culture liquid (2), host film (5), gusset (6) and drawstring (7), separated by gusset (6) between runner; Bioreactor culture liquid entrance (1) and runner nutrient solution entrance (8) forming reactions device nutrient solution entrance cavity (10), bioreactor culture liquid outlet (2) and runner nutrient solution export (9) forming reactions device nutrient solution outlet plenum (11); A drawstring (7) is provided with in described bioreactor culture liquid entrance cavity (10) and/or bioreactor culture liquid outlet plenum (11).Present invention optimizes the one-piece construction of bioreactor; Improve the work-ing life of the board-like bioreactor of software; Reducing bioreactor because of reasons such as aging, stress causes the danger of breaking.
Description
Technical field
The present invention relates to microdisk electrode photobioreactor field, particularly, the present invention relates to the board-like bioreactor of software of the enhancing of a kind of inside, multiple flow passages parallel connection.
Background technology
Along with the exhaustion day by day of fossil energy and the day by day serious of environmental pollution, people start to find new substitute energy.Luminous energy can be effectively utilized by H as one
2o, CO
2be converted into the photosynthetic autotrophs unicellular organism of organic resource with inorganic salt, micro-algae has the advantage that fat content is high, growth cycle is short, output is high, worldwide receives increasing concern, is considered to the most promising bioenergy raw material of one.In addition, micro-algae is also containing the necessary protein of the mankind, carbohydrate, polyunsaturated fatty acid and other biologically active substance.
The precondition of micro-algae industry development carries out pilot scale culture, thus obtain sufficient biomass material.Micro-algae large scale culturing can be divided into open and closed.Open microdisk electrode refers to various open pond, through years development, be applied to (BorowitzkaL.T. in the micro-algae large scale culturing of commercialization, BioresourceTechnology, 1991,38:251-252), the features such as it possesses skills simply, low investment, achieve good effect in the large scale culturing of spirulina, chlorella and salt algae.But there is culture condition instability in it, unit volume productive rate is low, the efficiency of light energy utilization is low, not easily keep the shortcomings such as purebred cultivation simultaneously.
Closed cultivation refers to the closed photo bioreactor adopting various ways (as pillar, tubular type and board-like).Compared with open cultivation, closed cultivation be not easy contaminated, saving water resource, culture density are high, results cost low, shortcoming is cost of investment high (PulzO., AppliedMicrobiologyandBiotechnology, 2001,57:287-293; LeeY-K., JournalofAppliedPhycology, 2001,13:307-315; TsygankovA.A., AppliedBiochemistryandMicrobiology, 2001,37 (4): 333-341).In order to overcome the high defect of closed toxigenic capacity, need on the one hand, by optimizing bioreactor design, to improve the efficiency of light energy utilization, thus increase substantially productive rate; Then need on the other hand to reduce the cost of investment of bioreactor and material, the energy consumption cost needed for cultivation.
The material manufactures such as traditional bioreactor many employings glass or stainless steel, their stability and weather resistance high, but high cost, is difficult to large-scale application.Therefore, low cost optical bio-reactor causes people and more and more payes attention to, and is more and more applied to large scale culturing (TrediciM.R.etal, BiotechnolBioeng, 2009,102, the 100-112. of micro-algae; BergmannP.etal, ChemIngTech, 2013,85,202-205; Willson, B., 2009, doi:10.1088/1755-1307/6/9/192015; YooJ.J.etal, BioprocessBiosystEng, 2013,36,729-736).This kind of bioreactor many employings software light transmission film material is made, and is easy to batch micro operations, and construction is simple, and compared with traditional bioreactor, cost greatly reduces.
The optimization of bioreactor, then mainly by the mixing of hot housing liquid, makes frustule high frequency between light district and dark space shuttle, realizes sparkle effect, thus improve the efficiency of light energy utilization of bioreactor and the output of frustule.Sparkle effect is the ubiquitous a kind of phenomenon of photoautotroph, namely, when frustule shuttles between the light district and dark space of bioreactor, the frustule accepting illumination just can be made to enter dark space in time and to carry out dark reaction, make the frustule completing dark reaction get back to light district simultaneously and again accept illumination, so just the photon entering bioreactor is fully utilized.If frustule with CF (frequency usually above 1Hz) when the light district of bioreactor and dark space frequent replacement, can produce " sparkle effect ", the utilization ratio of luminous energy can be greatly improved (JanssenM, SlendersP, TramperJ, etal., EnzymeMicrobialTechnology, 2001,29:298 ~ 305; MatthijsH.C.P, BalkeH, MurL.R, etal., BiotechnologyandBioengineering, 1996,50:98-107; XueS.Z, SuZ.F, CongW, JournalofBiotechnology, 2011,151 (3), 271-277).
Can the mixing of hot housing liquid by the design of certain internal structure, wherein in bioreactor, add static mixer or baffle plate, not only can the mixing of hot housing liquid, also can realize the sparkle effect of microalgae cell.Chinese patent CN201010193837.4 provides a kind of board-like bioreactor with specific internal, it makes algae liquid flow twist by arranging baffle plate at the plane of illumination of bio-reactor inwall and matte surface along nutrient solution flow direction, to realize frustule shuttling between the light district and dark space of bioreactor, play " sparkle effect " of frustule, thus improve the efficiency of light energy utilization that microalgae mass is cultivated and the output improving microalgae cell.But when making this kind of bioreactor of multiple runner with soft material, there is stressed concentrated position, reactor is easily broken, reduce the work-ing life of bioreactor, and the distance difference (as shown in CN201010193837.4 Figure of description 3) of each runner nutrient solution entrance distance bioreactor culture liquid entrance, cause nutrient solution flow velocity and flow condition in each runner variant, make the bioreactor internal shunt road efficiency of light energy utilization lower, thus the efficiency of light energy utilization of bioreactor entirety and the output of microalgae cell when have impact on microdisk electrode.
Summary of the invention
The object of the invention is to, for the problems referred to above, that a kind of inside strengthens, multiple flow passages parallel connection, the board-like bioreactor of software that each runner flow velocity is consistent is provided, with the board-like bioreactor of software solved described in patent CN201010193837.4 exist easily break, the problem such as nutrient solution flow velocity is variant in different runner.
The board-like bioreactor one-piece construction of software of the present invention as shown in Figure 1, except possessing the board-like bioreactor feature described in CN201010193837.4, i.e. a runner or at least two runners parallel with one another, each runner described comprises:
-at least 2 pieces of overhead gages 3, are arranged on the inner-wall surface of plane of illumination;
-at least 2 pieces of lower baffle plates 4, are arranged on the inner-wall surface of matte surface;
Wherein, the length direction of overhead gage and lower baffle plate and the corner dimension of nutrient solution flow direction are 20 ~ 70 degree, and angle direction is contrary;
Also comprise: bioreactor culture liquid entrance 1, bioreactor culture liquid outlet 2, host film 5, gusset 6, drawstring 7,
Separated by gusset 6 between described runner, described gusset 6 is that the host film 5 of plane of illumination and matte surface in the board-like bioreactor global formation of software is directly bondd or welds bond line or the soldered of formation, plays division plate effect.
Described bioreactor culture liquid entrance 1 and bioreactor culture liquid outlet 2 are diagonally arranged at software board-like bioreactor upper runner nutrient solution entrance 8 one end and runner nutrient solution exports 9 one end respectively, bioreactor culture liquid entrance 1 and runner nutrient solution entrance 8 forming reactions device nutrient solution entrance cavity 10, bioreactor culture liquid outlet 2 exports 9 forming reactions device nutrient solution outlet plenums 11 with runner nutrient solution;
Be provided with a drawstring 7 in described bioreactor culture liquid entrance cavity 10 and/or bioreactor culture liquid outlet plenum 11, described drawstring 7 is a software film, and its lower edges is directly bondd or is welded on bioreactor inwall.
On the board-like bioreactor host film 5 of described software, the material thickness at gusset 6 position is more than or equal to the material thickness at other positions.On described host film 5, the material thickness at gusset 6 position is preferably the 1.1-3 of other part material thickness doubly.
Described bioreactor culture liquid entrance cavity 10 is when being full of nutrient solution, and reduce gradually along the sectional area perpendicular to runner nutrient solution entrance 8 extended line direction along nutrient solution flow direction, the sectional area ratio at maximum and minimum place is 2:1-5:1.
Described bioreactor culture liquid outlet plenum 11 is when being full of nutrient solution, and increase gradually along the sectional area exporting 9 extended line directions perpendicular to runner nutrient solution along nutrient solution flow direction, the sectional area ratio at maximum and minimum place is 2:1-5:1.
The sectional area of described bioreactor culture liquid entrance cavity 10 reduces gradually along nutrient solution flow direction, avoid not have in Chinese patent CN201010193837.4 the liquid linear velocity at the two ends of entrance cavity in the scheme of reducing and pressure gap excessive (liquid linear velocity near one end place of bioreactor culture liquid entrance 1 and pressure very high, away from the liquid linear velocity at one end place of bioreactor culture liquid entrance 1 and pressure very low), flow velocity in each runner is inconsistent, the problem that the portion of runner efficiency of light energy utilization is lower, make the linear velocity of nutrient solution in photoreactor nutrient solution entrance cavity 10 of the present invention and pressure evenly, also make the nutrient solution flow velocity in the runner of each parallel connection of photoreactor of the present invention more balanced, in nutrient solution entrance cavity 10, the linear velocity of nutrient solution reduces the disturbance in (when nutrient solution enters the runner of each parallel connection from photoreactor nutrient solution entrance cavity 10) runner nutrient solution entrance 8 flow field and resistance to flow closer to the nutrient solution flow velocity in the runner of each parallel connection.The effect that the sectional area of described bioreactor culture liquid outlet plenum 11 increases gradually along nutrient solution flow direction is similar with it.
The height of described drawstring 7 is the 20%-80% of the width of the bioreactor culture liquid entrance cavity 10 at its place or the corresponding site of bioreactor culture liquid outlet plenum 11, wherein, the width of the corresponding site of the overall bioreactor culture liquid entrance cavity 10 that launches or bioreactor culture liquid outlet plenum 11 when described " width of corresponding site " refers to that bioreactor is not filled with nutrient solution, namely, the height of the drawstring of bioreactor culture liquid entrance cavity 10 reduces gradually along nutrient solution flow direction, and the height of the drawstring of bioreactor culture liquid outlet plenum 11 increases gradually along nutrient solution flow direction.
The length of described drawstring 7 is the 30%-80% of bioreactor width, wherein, and the overall width launched when described " bioreactor width " refers to that bioreactor is not filled with nutrient solution.
Described drawstring 7 can be complete software film, also can be the software film having one or more hole.The cross section in the hole on drawstring can be circle, rectangle, trapezoidal, trilateral or other shapes, and the distance of the diameter in described hole or the lower edges in hole is the 10%-70% of position, hole drawstring height.
Described overhead gage 3 and the cross section of lower baffle plate 4 can be rectangle, trapezoidal, trilateral, semicircle or other shape (as shown in Figure 5); Wherein, the thickness of the baffle plate of rectangular shape is 1-5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1-5mm.
Be 10-200mm along the spacing of the same face two adjacent screen on flow channel length direction in the board-like bioreactor of described software.
Overhead gage 3 in the board-like bioreactor of described software, lower baffle plate 4 are the 10%-100% of the board-like bioreactor width of flow path of this software along the projecting length in width of flow path direction, wherein, the width of the overall runner launched when described " bioreactor width of flow path " refers to that bioreactor is not filled with nutrient solution.
The height (i.e. the height in the cross section of baffle plate own) of overhead gage 3 of the present invention and lower baffle plate 4 is 1-15mm.
The material of main part (material of host film 5) of the board-like bioreactor of described software is software light transmissive material, and described software light transmissive material can be the one in polyethylene, polyvinyl chloride, polypropylene, polyester, thermoplastic elastomer etc.
The material of main part of the board-like bioreactor of described software can select printing opacity and ventilative material further, and preferably, material of main part is can pass through oxygen and not through the software light transmission film material of carbonic acid gas.
The thickness of described software light transmissive material is 0.1-2mm.
Gusset 6 place can be selected to add the manufacture of thick film according to Drawing abillity.Such as, strip software film can be bonded on the inner-wall surface at software bioreactor gusset 6 position, also can directly process the gusset position film different from other part thickness with mould.
Described drawstring material can be software light transmissive material or light-proof material, and described light transmissive material can be the one in polyethylene, polyvinyl chloride, polypropylene, polyester and thermoplastic elastomer etc.; Described light-proof material can be the one in plastics, rubber and resin etc.; Drawstring material and material of main part identical or different, preferably, the material of described drawstring is identical with the material of main part of bioreactor.
For reaching technique effect of the present invention, when bioreactor is not filled with the expansion of nutrient solution entirety, perpendicular on the cross section of nutrient solution flow direction in runner, the view field of described overhead gage and lower baffle plate can be overlapping, namely described overhead gage and lower baffle plate can be engaged mutually, depend on the spacing of adjacent screen; When bioreactor is full of nutrient solution, perpendicular on the cross section of nutrient solution flow direction in runner, the view field of described overhead gage and lower baffle plate is not overlapping, and namely described overhead gage and lower baffle plate can not be engaged mutually.
Described baffle plate can be solid construction or hollow structure.
The material of described baffle plate can be software light transmissive material or light-proof material, and described light transmissive material can be the one in polyethylene, polyvinyl chloride, polypropylene, polyester and thermoplastic elastomer etc.; Described light-proof material can be the one in plastics, rubber and resin etc.; Barrier material and material of main part identical or different, preferably, the material of described baffle plate is identical with the material of main part of bioreactor.
The board-like bioreactor baffle plate of software of the present invention and software light transmission film material can with boning, hot pressing, hight frequency welding, the method such as ultrasonic welding be bonded together, also can by calendering, squeeze sheet, the method such as compound, plastic uptake compound of extruding make baffle plate once shaped together with software light transmission film material.
The board-like bioreactor of software of the present invention is that reactor body material software light transmission film material (as coiled material or film material) is bonded together at gusset 6 place, drawstring place and surrounding edge place by the methods such as direct bonding, hot pressing, hight frequency welding, ultrasonic welding, and after being filled with nutrient solution, the softened thus Formation cross-section of runner is circular or oval runner.
When nutrient solution flows through the board-like bioreactor of described software, spiral motion can be formed in runner, and whirlpool can be produced between baffle plate, promote microalgae cell and form certain to-and-fro movement on optical path direction, thus realize microalgae cell shuttling in the light district of the board-like bioreactor of software and dark space, as shown in Figure 4.
Between the gusset of the board-like bioreactor of software of the present invention and gusset, between outermost gusset and reactor wall, form runner parallel with one another, bioreactor length, bioreactor width, the quantity of bioreactor flow channel length, bioreactor width of flow path and bioreactor runner can do corresponding adjustment according to actual needs, forms the board-like bioreactor of software of different profile.Wherein the quantity of runner is 2-50, preferred 5-30, most preferably 10-20.
When cultivating microalgae cell in the board-like bioreactor of software of the present invention, the flow velocity of nutrient solution in bioreactor runner is 0.1-1m/s, and described flow velocity refers to superficial linear vilocity.
When extensive micro-algae produces, according to the requirement of output, by board-like for multiple software of the present invention bioreactor parallel connection or can connect, then other device supporting.Such as, as shown in Figure 6 and Figure 7, cultivate in the process of microalgae cell in actual production, in whole culture systems except the board-like bioreactor 18 of above-mentioned software of the present invention, be also furnished with nutrient solution storing unit 16, nutrient solution e Foerderanlage 17, gas-liquid exchange device 19 is for air feed and carry out oxygen parsing, CO
2other auxiliary equipments such as supplementary device 20 and coupling device, material distribution device, temperature monitor and control device.
The micro-algae large-scale that the board-like bioreactor of software of the present invention is suitable under various climatope is cultivated; can be built in outdoor outdoor or booth; can directly lay or cover the tops such as top of building, Desertification Soil, sandy beach; the water surface such as river, lake, ocean can be swum in, make full use of natural light.Can cultivate various micro-algae, comprise one or more in spirulina, chlorella, salt algae, grid algae, diatom, chrysophyceae and plan microballoon algae.
Compared with the board-like bioreactor of CN201010193837.4, the advantage of the board-like bioreactor of software provided by the invention is:
1, optimize the one-piece construction of bioreactor, be provided with the bioreactor culture liquid entrance cavity 10 along the change of nutrient solution flow direction sectional area and bioreactor culture liquid outlet plenum 11, CFD software simulation is utilized to find the flow velocity basic equalization of algae liquid in different runner (as shown in Figure 3, in flow field simulation figure, the different colours depth represents different flow velocitys), in same each runner, the light district/dark space inversion frequency of frustule is basically identical (as shown in Figure 4, the movement locus of microalgae cell shown in Fig. 4), thus make the efficiency of light energy utilization of each runner of bioreactor basically identical, improve the optical energy utilization efficiency of bioreactor entirety.
2, bioreactor culture liquid entrance cavity 10 and bioreactor culture liquid outlet plenum 11 are provided with drawstring, the withstand voltage and impact resistance of the board-like bioreactor of software is greatly improved, improves the work-ing life of the board-like bioreactor of software.
3, the software film thickness at software board-like bioreactor gusset 6 place is more than or equal to other position films, improve the intensity at gusset place, reduce bioreactor and cause the danger of breaking because of reasons such as aging, stress, improve the work-ing life of the board-like bioreactor of software.
4, the board-like bioreactor of software of the present invention is owing to have employed software light transmission film material, and bioreactor cost is significantly reduced, and the about every square meter 50 yuan of batch cost, well below the general board-like bioreactor made of glass.
5, by board-like for multiple monomer software bioreactor serial or parallel connection, thus greatly operation and maintenance cost can be reduced; It can directly tile without hardened ground, can connect into culture systems, rapid construction fast, as shown in Figure 8; Especially, in Gobi Desert Terrain area, can the system of board-like for multiple monomer software bioreactor serial or parallel connection be directly overlayed on sand ground, on the one hand by the gap Accumulated Rainwater between monomer, reducing steam output on the one hand, is Gobi Desert Terrain area water-retaining effect.
Accompanying drawing explanation
Fig. 1 is the vertical view of the board-like bioreactor of software of the present invention.
Fig. 2 is drawstring of the present invention and installation site schematic diagram.
Fig. 3 is the flow field simulation figure in the board-like bioreactor of software of the present invention.
Fig. 4 is the change in location of frustule in the board-like bioreactor of software of the present invention.
Fig. 5 is the front view of several different cross section shape of single baffle plate on baffled film of the present invention.
Fig. 6 is the microalgae culture system schematic diagram adopting the board-like bioreactor series connection of multiple software of the present invention.
Fig. 7 is the microalgae culture system schematic diagram adopting the board-like bioreactor parallel connection of multiple software of the present invention.
Fig. 8 is the microalgae culture system adopting the board-like bioreactor series connection of multiple software of the present invention.
Accompanying drawing identifies
1, bioreactor culture liquid entrance 2, bioreactor culture liquid outlet 3, overhead gage
4, lower baffle plate 5, host film 6, gusset
7, drawstring 8, runner nutrient solution entrance
9, runner nutrient solution outlet 10, bioreactor culture liquid entrance cavity
11, bioreactor culture liquid outlet plenum 12, rectangle baffle plate 13, triangular baffle
14, trapezoidal baffle plate 15, Semicircular baffle 16, nutrient solution storing unit
17, nutrient solution e Foerderanlage 18, the board-like bioreactor of software
19, gas exchange device 20, CO
2supplementary device
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
Embodiment 1
The cultivation of spirulina is carried out under outdoor natural light shines.The culture systems built comprises a board-like bioreactor of software 18, nutrient solution storing unit 16, nutrient solution e Foerderanlage 17 (commercially available surge pump), gas-liquid exchange device 19 and CO
2supplementary device 20, mode of connection as shown in Figure 6.
The structure of the board-like bioreactor 18 of software as shown in Figure 1.On the ground, main material is software printing opacity polyethylene film to the board-like bioreactor tiling of software.The long 200cm of the board-like bioreactor of software, wide 96cm, be divided into the runner that 16 parallel with one another; Host film 5 thickness is 0.5mm, gusset 6 place film thickness 0.75mm, and the length of gusset 6 is 140cm; The sectional area ratio at bioreactor culture liquid entrance cavity 10 and bioreactor culture liquid outlet plenum 11 maximum and minimum place is 2:1; Overhead gage 3 and lower baffle plate 4 are rectangle baffle plate, highly for 10mm, width be 2.5mm, length is 6mm, be 30 ° with fluid flowing angle, in each runner, 12 pieces of overhead gages and 12 pieces of lower baffle plates are installed; Drawstring 7 is provided with 4 circular apertures, and the length of drawstring 7 is 48cm, and the height of drawstring 7 is 50% of the width of the bioreactor culture liquid entrance cavity 10 at its place or the corresponding site of bioreactor culture liquid outlet plenum 11.
Algae kind is from Chinese Academy Of Sciences Process Engineering Research Institute, and kind is spirulina plalensis, and substratum is Zarrouk substratum, and in nutrient solution, the starting point concentration of sodium bicarbonate is 0.1mol/L.
Above-mentioned bioreactor is cleaned with the water after 0.2 μm of micro-filtrate membrane filtration before cultivating.According to Zarrouk culture medium prescription preparation 50L substratum, substratum 0.2 μ micro-filtrate membrane filtration.Preparation kind of liquid according to a conventional method, inoculum density 0.1g/L.Medium pH value is set in 9.0, and envrionment temperature 25 ~ 35 DEG C, adopts outdoor natural lighting, and controlling culture temperature is 26 ± 1 DEG C.The flow velocity of nutrient solution in bioreactor runner is 20cm/s, and the apparent pressure at bioreactor nutrient solution entrance 1 place is 6kPa.Between incubation period, in the gas-liquid exchange device 19 in culture systems, pass into air, carry out the releasing of dissolved oxygen, make bioreactor culture liquid exit nutrient solution saturation dissolved oxygen lower than 300%; Carbon mode is mended to CO by pH value feedback
2cO is passed in supplementary device 20
2.Period sampling measuring algae cell density, semicontinuous gathering is started when algae cell density reaches 3g (dry weight)/L, collecting method is that (approximately every 3 days) draw 20% of nutrient solution in culture systems, is returned in former culture systems by the filtered liquid after filtering.Filter the frustule obtained and gather in the crops rear washing, drying.
Between incubation period, the concentration of other nutritive salt of periodic detection is also supplemented in time, and supplements a small amount of water to make up evaporation of water loss.Culturing process continues 30 days, and photo-bioreactor system still can normally run, and the area yield of spirulina is about 40g/m
2.d.
Comparative example 1
The cultivation of spirulina is carried out under outdoor natural light condition.Other conditions with embodiment 1, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.
Culturing process continues 5 days, and when the 5th day, bioreactor breaks, and the area yield of spirulina is 33g/m
2.d.
Comparative example 1 and comparative example 1 known, the inside of the embodiment of the present invention 1 strengthens software board-like bioreactor work-ing life is higher than the work-ing life of the bioreactor of comparative example 1; And under same culture condition, the embodiment of the present invention 1 be provided with area yield in the bioreactor culture liquid entrance cavity of sectional area change and the bioreactor of bioreactor culture liquid outlet plenum higher than the area yield in the bioreactor of comparative example 1.Work-ing life of bioreactor of the present invention and improving obviously for the efficiency of light energy utilization of frustule during microdisk electrode.
Embodiment 2
Outdoor natural light according under carry out the cultivation of spirulina, other condition with embodiment 1,2 times of difference to be bioreactor gusset 6 part thickness be other part thickness.
Culturing process continues 60 days, and bioreactor still can normally run, and the area yield of spirulina is about 39g/m
2.d, bioreactor work-ing life is far above the bioreactor work-ing life in comparative example 1; Area yield is higher than the area yield in the bioreactor in comparative example 1.Work-ing life of bioreactor of the present invention and significantly improving for efficiency of light energy utilization during microdisk electrode.
Embodiment 3
Under outdoor natural light shines, carry out the cultivation of spirulina, other condition is with embodiment 1, and difference is the sectional area ratio at bioreactor culture liquid entrance cavity 10 and bioreactor culture liquid outlet plenum 11 maximum and minimum place in bioreactor is 4:1.
Culturing process continues 30 days, and bioreactor still can normally run, and the area yield of spirulina is about 37g/m
2.d, bioreactor work-ing life is far above the bioreactor work-ing life in comparative example 1; Area yield is higher than the area yield in the bioreactor in comparative example 1.Work-ing life of bioreactor of the present invention and significantly improving for efficiency of light energy utilization during microdisk electrode.
Embodiment 4
The cultivation of spirulina is carried out under outdoor natural light shines, other condition is with embodiment 1, difference is the width of drawstring 7 in bioreactor is 70% of bioreactor width, and the height of drawstring 7 is 60% of the width of the bioreactor culture liquid entrance cavity 10 at its place or the corresponding site of bioreactor culture liquid outlet plenum 11.
Culturing process continues 30 days, and bioreactor still can normally run, and the area yield of spirulina is about 38g/m
2.d, bioreactor work-ing life is far above the bioreactor work-ing life in comparative example 1; Area yield is higher than the area yield in the bioreactor in comparative example 1.Work-ing life of bioreactor of the present invention and significantly improving for efficiency of light energy utilization during microdisk electrode.
Embodiment 5
The cultivation of spirulina is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference is the flow velocity of nutrient solution in bioreactor is 50cm/s.
Culturing process continues 30 days, and bioreactor still can normally run, and the area yield of spirulina is about 45g/m
2.d.Under more high flow rate and pressure, bioreactor still can normally run for a long time, and area yield is apparently higher than the area yield of the bioreactor in comparative example 1.Work-ing life of bioreactor of the present invention and significantly improving for efficiency of light energy utilization during microdisk electrode.
Embodiment 6
The cultivation of spirulina is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference is the long 200cm of the board-like bioreactor of software 18, wide 100cm, is divided into the runner that 10 parallel with one another.Culturing process continues 30 days, and the area yield of spirulina is about 35g/m
2.d.
The software board-like bioreactor work-ing life that the inside of the embodiment of the present invention 6 strengthens and area yield all higher than work-ing life and the area yield of the bioreactor of comparative example 2, namely bioreactor of the present invention work-ing life and improve obviously for the efficiency of light energy utilization of frustule during microdisk electrode.
Comparative example 2
The cultivation of spirulina is carried out under outdoor natural light condition.Other conditions with embodiment 6, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.
Culturing process continues 8 days, and when the 8th day, bioreactor breaks, and the area yield of spirulina is 30g/m
2.d.
Embodiment 7
The cultivation of chlorella is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference adopts BG11 substratum, utilizes CO
2as carbon source.Culturing process continues 30 days, and the area yield of chlorella is about 25g/m
2.d.
Obviously, work-ing life in the bioreactor of the embodiment of the present invention 7 and area yield, apparently higher than work-ing life of the bioreactor of comparative example 3 and area yield, show that the work-ing life of bioreactor of the present invention and the efficiency of light energy utilization significantly improve.
Comparative example 3
The cultivation of chlorella is carried out under outdoor natural light condition.Other conditions as embodiment 7, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.Culturing process continues 5 days, and the area yield of chlorella is about 20g/m
2.d.
Embodiment 8
The cultivation of grid algae is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference adopts BG11 substratum, utilizes CO
2as carbon source.Culturing process continues 30 days, and the area yield of grid algae is about 45g/m
2.d.
Obviously, work-ing life in the bioreactor of the embodiment of the present invention 8 and area yield, apparently higher than work-ing life of the bioreactor of comparative example 4 and area yield, show that the work-ing life of bioreactor of the present invention and the efficiency of light energy utilization significantly improve.
Comparative example 4
The cultivation of grid algae is carried out under outdoor natural light condition.Other conditions as embodiment 8, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.Culturing process continues 5 days, and the area yield of grid algae is about 37g/m
2.d.
Embodiment 9
The cultivation intending microballoon algae is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference adopts artificial seawater+1/2F substratum, utilizes CO
2as carbon source.Culturing process continues 30 days, and the area yield intending microballoon algae is about 22g/m
2.d.
Obviously, work-ing life in the bioreactor of the embodiment of the present invention 9 and area yield, apparently higher than work-ing life of the bioreactor of comparative example 5 and area yield, show that the work-ing life of bioreactor of the present invention and the efficiency of light energy utilization significantly improve.
Comparative example 5
The cultivation intending microballoon algae is carried out under outdoor natural light condition.Other conditions as embodiment 9, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.Culturing process continues 5 days, and the area yield intending microballoon algae is about 18g/m
2.d.
Embodiment 10
The cultivation of spirulina is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference is the board-like bioreactor 18 of above-mentioned software that system comprises 50 series connection.Culturing process continues 30 days, and the area yield of spirulina is about 38g/m
2.d.
Comparative example 10 and comparative example 6 known, under more extensive microalgae culture system, still can normally run at the board-like bioreactor of software of the present invention, and there is higher area yield, and the bioreactor extensive disruption of comparative example 6, show that bioreactor of the present invention significantly improves work-ing life, may be used for the microdisk electrode of more large scale system.
Comparative example 6
Other conditions with embodiment 10, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.After water-filling, break in bioreactor many places, cannot carry out microdisk electrode.
Embodiment 11
The cultivation of spirulina is carried out under outdoor natural light shines.Other condition is with embodiment 1, and difference is that system comprises 30 board-like bioreactors 18 of above-mentioned software in parallel.Culturing process continues 30 days, and the area yield of spirulina is about 38g/m
2.d.
Comparative example 11 and comparative example 7 known, have longer work-ing life and higher area yield at the board-like bioreactor of software of the present invention, namely bioreactor work-ing life of the present invention and the efficiency of light energy utilization significantly improve.
Comparative example 7
Other conditions with embodiment 11, the difference bioreactor culture liquid entrance cavity that to be bioreactor be wherein as shown in CN201010193837.4 accompanying drawing 3 and bioreactor culture liquid outlet plenum sectional area unchanged, drawstring is not set, bioreactor that gusset position does not strengthen.Culturing process continues 5 days, and part bioreactor starts to break, and the area yield of spirulina is about 38g/m
2.d.
Claims (14)
1. the board-like bioreactor of software, comprise a runner or at least two runners parallel with one another, each runner described comprises:
-at least 2 pieces of overhead gages (3), are arranged on the inner-wall surface of plane of illumination;
-at least 2 pieces of lower baffle plates (4), are arranged on the inner-wall surface of matte surface;
Wherein, the length direction of overhead gage (3) and lower baffle plate (4) and the corner dimension of nutrient solution flow direction are 20 ~ 70 degree, and angle direction is contrary;
The board-like bioreactor of described software also comprises bioreactor culture liquid entrance (1), the outlet of bioreactor culture liquid (2), host film (5), gusset (6) and drawstring (7), it is characterized in that, separated by gusset (6) between described runner, described gusset (6) is directly bondd or is welded bond line or the soldered of formation for the host film (5) of plane of illumination and matte surface in the board-like bioreactor global formation of software;
Described bioreactor culture liquid entrance (1) and bioreactor culture liquid outlet (2) are diagonally arranged at software board-like bioreactor upper runner nutrient solution entrance (8) one end and runner nutrient solution outlet (9) one end respectively, bioreactor culture liquid entrance (1) and runner nutrient solution entrance (8) forming reactions device nutrient solution entrance cavity (10), bioreactor culture liquid outlet (2) and runner nutrient solution export (9) forming reactions device nutrient solution outlet plenum (11);
A drawstring (7) is provided with in described bioreactor culture liquid entrance cavity (10) and/or bioreactor culture liquid outlet plenum (11), described drawstring (7) is a software film, and its lower edges is directly bondd or is welded on bioreactor inwall.
2. the board-like bioreactor of software according to claim 1, is characterized in that, the material thickness at upper gusset (6) position of the board-like bioreactor host film (5) of described software is more than or equal to the material thickness at other position.
3. the board-like bioreactor of software according to claim 2, is characterized in that, the material thickness at upper gusset (6) position of described host film (5) is 1.1-3 times of other part material thickness.
4. the board-like bioreactor of software according to claim 1, it is characterized in that, described bioreactor culture liquid entrance cavity (10) is when being full of nutrient solution, reduce gradually along the sectional area perpendicular to runner nutrient solution entrance (8) extended line direction along nutrient solution flow direction, the sectional area ratio at maximum and minimum place is 2:1-5:1.
5. the board-like bioreactor of software according to claim 1, it is characterized in that, described bioreactor culture liquid outlet plenum (11) is when being full of nutrient solution, increase gradually along the sectional area perpendicular to runner nutrient solution outlet (9) extended line direction along nutrient solution flow direction, the sectional area ratio at maximum and minimum place is 2:1-5:1.
6. according to the described board-like bioreactor of software arbitrary in claim 1,4 or 5, it is characterized in that, the height of described drawstring (7) is the 20%-80% of the corresponding site width that the bioreactor culture liquid entrance cavity (10) at its place or bioreactor culture liquid outlet plenum (11) launch when not being filled with nutrient solution.
7. according to the described board-like bioreactor of software arbitrary in claim 1,4 or 5, it is characterized in that, the length of described drawstring (7) is the 30%-80% of the width that board-like bioreactor launches when not being filled with nutrient solution.
8., according to the described board-like bioreactor of software arbitrary in claim 1,4 or 5, it is characterized in that, described drawstring (7) is complete software film, or has the software film in one or more hole.
9. the board-like bioreactor of software according to claim 8, is characterized in that, the distance of the diameter in described hole or the lower edges in hole is the 10%-70% of position, hole drawstring height.
10. the board-like bioreactor of software according to claim 1, is characterized in that, the material of described host film (5) is software light transmissive material; The material of described overhead gage (3), lower baffle plate (4) or drawstring (7) is software light transmissive material or software light-proof material.
The board-like bioreactor of 11. software according to claim 10, is characterized in that, described software light transmissive material is the one in polyethylene, polyvinyl chloride, polypropylene, polyester or thermoplastic elastomer; Described software light-proof material is the one in plastics, rubber or resin.
The board-like bioreactor of 12. software according to claim 1, is characterized in that, is 10-200mm along the spacing of the same face two adjacent screen on flow channel length direction in the board-like bioreactor of described software.
The board-like bioreactor of 13. software according to claim 1, it is characterized in that, in the board-like bioreactor of described software overhead gage (3) and lower baffle plate (4) along the projecting length in width of flow path direction be the board-like bioreactor of this software be not filled with nutrient solution time the 10-100% of width of flow path that launches.
The board-like bioreactor of 14. software according to claim 1, is characterized in that, described overhead gage (3) or the cross-sectional height of lower baffle plate (4) are 1-15mm.
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