CN103361257A - Light direction hybrid reinforcement-based novel internal component and photobioreactor - Google Patents
Light direction hybrid reinforcement-based novel internal component and photobioreactor Download PDFInfo
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Abstract
The invention provides a light direction hybrid reinforcement-based novel internal component and a photobioreactor. The photobioreactor comprises a transparent housing; a plurality of transparent clapboards which are regularly distributed horizontally and slantwise are arranged along the axial direction in the housing, ladder-shaped local space areas are formed among the clapboards, and the fluid of the center region (a light dark region) of the reactor and the fluid of the surface region (a lighting region) of the reactor generate quick and periodic cyclic motion, so that the hybrid of the fluid along the radial direction (the lighting direction) of the reactor can be enhanced. The light direction hybrid reinforcement-based novel internal component and the photobioreactor provided by the invention have the advantages that the hybrid of the fluid (the alga liquid) along the lighting (light decay) direction can be enhanced, the light dark cycle frequency of the alga cell can be improved, and the light can be abundantly used by the alga cell, various problems when the photobioreactor is amplified can be effectively overcome, and the photobioreactor provided by the internal component can be amplified along the optical path direction.
Description
Technical field
The invention belongs to the Microalgae biotechnology field, relate to a kind of bioreactor and internal mix member thereof of novelty, can realize the cultivation of little algae high-density and high yield.In addition, the present invention also can be used for other cultivation or chemical reaction field (such as photosynthetic bacterium cultivation, culture plant cell, light-catalyzed reactions etc.) that need illumination to carry out.
Background technology
The multiple high added value materials such as little algae rich in proteins, polysaccharide, polyunsaturated fatty acid and carotenoid, little algae has the ability that absorbs the N/P element and accumulate grease in culturing process simultaneously, therefore, little algae has widespread use in all many-sides such as bioenergy, biological solid carbon, environmental protection, food, feed and medicine.Particularly in recent years aspect bioenergy, little algae is considered to the raw material of the biofuels such as the biofuel preparation of at present tool application prospect.
Bioreactor is the core apparatus that little algae light autotrophy is cultivated.Bioreactor the performance good and bad directly little algae cultured cells density of impact and productive rate.At present, be used for bioreactor that little algae cultivates open type and closed two kinds are arranged.The open type bioreactor mainly refers to open raceway pond and circle pond, and they are most widely used type of reactor during the outdoor mass-producing of current little algae is cultivated.Build easy, easy to operate and the advantage such as running cost is low although the open type bioreactor exists, also have many shortcomings, low such as algae cell density and productive rate, culture condition is wayward, be subjected to easily the pollution such as protozoon.Another kind of bioreactor type is the closed photo reactor, compares with the open type bioreactor, and it has that culture density height, cell yield are high, culture condition is easy to control and is not subject to the advantage such as protozoon pollution.Therefore, closed photo bioreactor has widespread use aspect little algae high-density culture, little algae production high added value material and the experimental study.Especially; the in recent years mass-producing of the little algae of the energy is cultivated needs a large amount of algae kinds; and traditional Da Chi step by step the mode of spreading cultivation exist the cycle long, be easy to problems such as contaminated (can cause spreading cultivation unsuccessfully); thereby; adopt closed photo bioreactor as the seed of the little algae of the energy (can not heterotrophism cultivate or heterotrophism is cultivated the extremely slow algae kind of the speed) device that spreads cultivation; the risk of failure shortens simultaneously seed and spreads cultivation the cycle but the little algae seed of the decrease energy spreads cultivation, and improves the little algae culture efficiency of the energy.
At present, closed photo bioreactor mainly contains pillar, tubular type and flat three types.The tubular type bioreactor has larger specific surface area, generally can obtain higher algae cell density and productive rate.But pipeline reactor exists and mixes ability, serious, the inner wall washing and difficult in maintenance of dissolved oxygen accumulation.The pillar bioreactor is compared the tubular type bioreactor, has to mix, and oxygen is resolved the advantages such as easy, is its subject matter but amplify, and is to amplify all relatively difficulty in vertical or radial direction; Flat photobioreactor has that the light application ratio surface-area is large, light path is short, oxygen is resolved advantages such as easily, relatively being easy to amplification.In addition, dull and stereotyped optical-biological reaction can be adjusted the placed angle of dull and stereotyped reactor according to the sunlight angle, so that reactor is fully accepted illumination.Therefore, flat bioreactor is a class bioreactor of at present domestic and international each R﹠D institution and company's emphasis research and development.
In recent years, domestic and international document and patent of invention about Flat photobioreactor mainly contains:
1) Degen etc. has reported a kind of air lift type Flat photobioreactor (volume 1.5L of built-in staggered floor horizontal positioned dividing plate, width 1.5cm), its fluid decline passway is positioned at a side of reactor width, and rising passway is divided into 6 parts that communicate by 5 horizontal baffles.These 5 baffle plates alternately are fixed on the front and back panel of reactor, and fluid can form whirlpool when rising between two baffle region, so that frustule periodic cycle between light district and dark space.(Degen?J，Uebele?A，Retze?A，et?al.A?novel?airlift?photobioreactor?with?baffles?for?improved?light?utilization?through?the?flashing?light?effect.Journal?of?Biotechnology，2001，92(2)：89-94)。
2) Tredici etc. has designed a kind of flat bioreactor with EXS similar plastics bag form of being made by the transparent soft material, and it is referred to as Green Wall Panel.This design is so that the manufacturing cost decrease of bioreactor is fit to be applied to low-cost cultivate (WO2004/074423A2) of little algae.
3) the Solix company of the U.S. has developed a kind of board-like bioreactor that places water, its main body is bag film, place water, outside water not only can support bag reactor, also plays good temperature control and the effect of light distribution (US2008/0160591 A1).
4) Li Yuanguang etc. has invented the flat bioreactor of a kind of new more piece, has a plurality of hollow flow-guiding baffle plate cavitys in its reactor, and inside cavity can be put source of artificial light, between each flow apron cavity the interval is arranged.This reactor is owing to strengthen frustule with the mixing of fluid at direction of illumination, thereby can significantly improve the light utilising efficiency, avoided simultaneously the inner light source fluorescent tube directly to contact (CN1880442) with nutrient solution.
5) Hu etc. has invented a kind of at the extendible Flat photobioreactor of length direction.This reactor is comprised of a plurality of ordinary flat formula reactor units, is connected by plastics or metallic bond connection member between each unit.Reactor can be made into different in width (light path) as required, can arrange by linearity or curve form, can carry out differing tilt angles according to the sunlight angle simultaneously and place, and fully accepts illumination.(US2010/0028976A1)。
6) the Subitec company of Germany has designed a kind of dull and stereotyped airlift photobioreactor, each unit volume of this reactor is 180L, it is staggered mutually by two-layer PVC film and is connected to form groove, in each groove chamber, form fluid vortex after the air-blowing of bottom, it is so that the alternating frequency increase in light district and dark space of frustule, thereby improve cell growth rate (Morweiser M, Kruse O, Hankanmer B, et al.Developments and perspectives of photobioreactors for biofuel production.Applied microbiology and biotechnology, 2010,87:1291～1302).
At present, the subject matter that runs in actual applications of closed photo bioreactor has following 2 points: 1) make and running cost high; 2) amplify difficulty.From above-mentioned in recent years flat bioreactor Development Trend as seen, for the high problem of cost, generally improve from the making material aspect of reactor, the mechanically resistant materials such as in the past synthetic glass and simple glass are developed into the soft materials such as plastics film, like this can so that the cost of manufacture of reactor obviously descend.In addition, lighter soft materials also makes things convenient for reactor to move.Amplifying hard problem for bioreactor, still do not have at present good technique means, is to increase the integral installation liquid measure that the mini-reactor element number improves reactor when scale is amplified generally.
It is more that the internal mix member is used in the duct type bioreactor, and normally multi-form static mixer can be so that the fluid state in the pipeline reactor becomes turbulent flow from the laminar flow of routine, to improve the degree of mixing of algae liquid in the reactor.And general use less of internals internals in flat and column reactor, general one or two baffle plates, the dull and stereotyped reactor of formation iris type or air lift type installed in flat-plate reactor; The general guide shell of installing forms air lift type pillar bioreactor in column reactor.More than these internals usually can so that in the reactor fluid flow become in order, can promote to a certain extent frustule growth.Fluid is the effect of continuous circulation motion in the optical attenuation direction but this internals lacks, can not the mixing of enhance fluid on direction of illumination, and improve frustule and accept the limited in one's ability of illumination.
Therefore, this area still needs the bioreactor that is subjected to light intensity and promotion frustule light autophyting growth of a kind of luminous energy that can make frustule take full advantage of limited area interior (light district) in the reactor, raising frustule.
Summary of the invention
The present invention is with the design concept that is mixed into of strengthening fluid (micro algae culturing liquid) illumination in bioreactor (optical attenuation) direction, emphatically from improve reactor frustule to the dark cycle frequency angle of the light that takes full advantage of, improves frustule of luminous energy, a kind of bioreactor and internals thereof of internal structure novelty have been designed, not only can realize high-density and the cultivation of high-level efficiency light autotrophy of little algae, and can realize that bioreactor is in effective amplification of light path direction.
Therefore, the purpose of this invention is to provide a kind of can be so that fluid (algae liquid) produces cyclial movenent at direction of illumination (optical attenuation direction), thereby so that frustule takes full advantage of the luminous energy of limited area interior (light district) in the reactor, improve the light intensity that is subjected to of frustule.The present invention not only can so that in the reactor fluid flow more orderly, simultaneously can greatly promote fluid in the mixture strength of direction of illumination, so that frustule in reactor the light district and dark space (because optical attenuation due to) between quick travel, improve the dark cycle frequency of light of frustule, can greatly promote the light autophyting growth of frustule
Particularly, the invention provides a kind of novel bioreactor internals and corresponding bioreactor, it is characterized in that described bioreactor comprises:
Transparent outer hull;
In described housing (is the main body flow path direction of algae liquid axially, be the flat of placement perpendicular to the ground such as housing, then for short transverse) be mounted with the regularly arranged lamina of septum pellucidum that is horizontal tilt of polylith, described dividing plate and shell wall side form certain angle, form the local space zone of trapezoidal shape between neighbouring dividing plate and the housing, this configuration makes between the fluid in the fluid in reactor center zone (light dark space) and reactor surface zone (area of illumination) and produces fast cyclial movenent, has strengthened the mixing of fluid at reactor radial direction (direction of illumination); With
Supply gas device or other power set.
In a specific embodiment, described transparent outer hull is flat, column type, duct type and other shape.
In a specific embodiment, described internals is the regularly arranged lamina of septum pellucidum that is horizontal tilt of polylith, forms the local space zone of trapezoidal shape between each dividing plate.Described dividing plate can hollow out the formation hollow cavity.
In a specific embodiment, described device of air or other power set of giving is air pump, gas distributor or other relevant power-equipment that can cause algae liquid to flow in bioreactor.
In a specific embodiment, described transparent shell can have synthetic glass, unorganic glass (simple glass or toughened glass etc.) etc. to have the transparent material of certain physical strength; Or by making such as flexible materials such as overlays.
In a specific embodiment, described bioreactor internals can be that the solid transparent dividing plate also can be the cavity body dividing plate.If dividing plate is the transparent solid plate, then can split with shell of reactor, dividing plate can freely be inserted in the reactor; If dividing plate is the cavity body dividing plate, then its needs and shell of reactor are integrated.
In a specific embodiment, describedly hollow out rear formation in the hollow cavity by dividing plate and can place source of artificial light.
In a specific embodiment, described bioreactor also can comprise temperature detection and Controlling System, pH detection and feedback control system, dissolved oxygen detection system and inner light intensity detection system.
The present invention also relates to the application in the fields (comprising that other light requirement shines into capable cultivation or chemical reaction, such as photosynthetic bacterium cultivation, culture plant cell, light-catalyzed reaction etc.) such as little algae light autotrophy cultivation of above-mentioned bioreactor and internals thereof on the other hand.
The bioreactor with novel internals of the present invention design (take outer hull as flat as example) and conventional flat-plate reactor are (without inner member, bubbling style) compares and have liquid motion and reach in order direction of illumination and mix the advantages such as violent, frustule growth velocity and algae cell density frustule growth velocity and the algae cell density (seeing embodiment 1) in the dull and stereotyped reactor of routine.
Although the air lift type Flat photobioreactor of the built-in staggered floor dividing plate of the designs such as above-mentioned Degen also can promote fluid to flow the small only 1.5L of reactor in the direction of illumination loop cycle; Internal partition is horizontal positioned, and flow field unreasonable (reactor dividing plate of the present invention has certain angle of inclination, so that the flow field is more reasonable) can cause more dead band; And dividing plate can't split with outer hull, so that reactor cleans difficulty.In addition, the reactor of the designs such as Degen is individual layer in width (optical path or light path) direction, narrower, can't be wide as much as possible on optical path direction (being direction of illumination), be unfavorable for the amplification of this reactor on the optical path direction (reactor of the present invention's design has symmetrical two portions at width, can so that reactor can be wide as much as possible at width).
The air lift type Flat photobioreactor of Subitec company research and development equally also exists the cleaning difficult problem, especially cultivates for the little algae that is easy to attached wall.The internals of the present invention's design can split with reactor shell, conveniently reactor is thoroughly cleaned.
The dull and stereotyped reactor of Tredici design is mainly considered from reducing the reactor cost of manufacture, adopt film type soft plastic material to make reactor shell, externally with materials such as using simple very thin wire or plastic wire soft housing is supported simultaneously, and the inside of reactor there is no internals, the same with the dull and stereotyped reactor of routine, do not have the ability that promotes that algae liquid mixes at direction of illumination.
Equally, the Flat photobioreactor that can expand at length direction of Hu invention also be conventional dull and stereotyped reactor, and internal flow mixes comparatively chaotic and unordered, is unfavorable for the frustule growth.The invention of Hu is mainly considered from the scaling-up angle, but amplifying mainly, it sets about to increase the mini-reactor element number in the reactor length direction, and be not the amplification of single reactor volume on the traditional sense.
The film flat-plate reactor that places water of Solix company design says that from mixing angle it also is simple bubbling style type, and flow field mixture is unordered.And this reactor places water body, a little less than reactor shows that light intensity, is unfavorable for the cultivation of little algae of the high light intensity of needs.
The reactor of the present invention's design is mainly considered from the fluid angle, to strengthen frustule in the mixing of direction of illumination, the raising frustule is purpose to the utilising efficiency of luminous energy, the material of reactor can be the transparent materials such as synthetic glass or unorganic glass, also can be the soft materials such as plastics film, and the amplification of reactor both can from length direction, also can be amplified from width.The multi-section flat type photo-bioreactor (CN1880442) that in the past designed, although also having, it promote fluid flowing radially, but because fluid formation is periodically whole circulation, so that fluid is very long at the loop cycle of direction of illumination, frequency is lower, is not enough to form " sparkle effect " favourable to frustule.The present invention is further developing of multi-section flat type reactor, by in reactor, increasing the dividing plate that horizontal tilt is placed, form the local space of a plurality of trapezoidals, loop cycle is mobile faster to cause fluid to form cycle frequency in each local space, promotes frustule to the abundant acceptance of illumination.
Summarize described, the present invention compares with other bioreactor, consider from the mixing of strengthening fluid direction of illumination in reactor that emphatically so that reactor is in the amplification of light path direction, this amplifies for bioreactor and light autotrophy of little algae is cultivated very crucial.From reducing the cost angle, the reactor of the present invention's design can adopt cheap flexible film material to make; From amplifying angle, the reactor of the present invention's design both can also can amplify from length direction and short transverse from width (being the light path direction).
In a word, poor for existing closed photo bioreactor direction of illumination mixed performance, amplify the shortcomings such as difficulty, the present invention is by (being the main body flow path direction of algae liquid axially in reactor shell, be the flat of placement perpendicular to the ground such as housing, then for short transverse) be mounted with the method for the regularly arranged lamina of septum pellucidum that is horizontal tilt of polylith, come strengthening fluid in the mixing of reactor radial direction (direction of illumination), but so that being installed, the bioreactor of this new internal member has amplification in the light path direction, can so that fluid has strong loop cycle in illumination side, promote the unique advantages such as frustule Fast Growth simultaneously.
The accompanying drawing summary
Fig. 1 shows that internals is applied to the formed Novel series bioreactor of flat bioreactor (side-view).Wherein each symbol implication is as follows: w is the width of reactor, h is the height of reactor, h0 is that ventpipe is from the distance of bottom, h1 is along the distance along (type a and c) from reactor bottom under (type b) and the baffle plate under the dividing plate, h2 is along the distance along (type a and c) from the bottom under (type b) and the dividing plate on the dividing plate, h3 is the distance between two dividing plates, h4 is the distance along (type a and c) from reactor head on dividing plate (type b) and the baffle plate, w1 is the spacing (type b) of the horizontal direction between two dividing plates, w2 is the spacing (type a and c) of dividing plate and reactor wall, w3 is the distance (type c) between two baffle plates, α 0 is the crossing angle of dividing plate and wall.
Fig. 2 shows the pictorial diagram of the flat bioreactor of new internal structure and control reactor.
Fig. 3 shows the interior flow field (the hydromeehanics computed in software obtains as calculated) of the dull and stereotyped photoreactor of new internal structure and control reactor.
Fig. 4 shows that the chlorella culturing process of the dull and stereotyped reactor of new internal structure and control reactor compares.
Specific embodiments
Generally speaking, only affect the factor of frustule light autophyting growth most critical.Frustule meeting absorb light in the bioreactor, mutually blocking between the while frustule, so that light is exponential attenuation along with the increase of light path (medium depth) and cell concn, therefore, exist light district (light intensity is satisfied with the zone of frustule growth) and dark space (light intensity is lower than the required zone of frustule growth) in the bioreactor.Strengthen the travel frequency of frustule between light district and dark space, be conducive to frustule illumination, the growth velocity of raising frustule accepted as much as possible.This phenomenon is called " sparkle effect " (Flashing Light Effect).Therefore, the dark cycle frequency of light becomes an important parameter of bioreactor design, improve the interior alternation of light and darkness frequency of reactor dual mode is generally arranged: 1) improve mixture strength in the reactor, frustule can be finished from the dark space in the short period of time to the movement in light district; 2) internals (such as static mixer, baffle plate etc.) is set in reactor, reactor is divided into a plurality of little cycling elements, rely on the whirlpool of fluid flow generation so that frustule carries out the motion of cyclic periodicity between each junior unit, thereby improve the alternation of light and darkness frequency of frustule.
The present invention has designed a kind of internals of novelty, mainly be a series of dividing plates by certain regular arrangement, can cause algae liquid to be periodic rapid movement at direction of illumination, strengthen frustule in the mixing of direction of illumination, improve the dark cycle frequency of light of frustule, thereby improve the growth velocity of frustule.
Specifically, the invention provides a kind of internals and corresponding bioreactor of novelty, described bioreactor comprises transparent housing; Be mounted with many groups lamina of septum pellucidum of arranging with rule in described enclosure interior, they form has similar trapezoidal local space zone, can make between the fluid in the fluid in reactor center zone (light dark space) and reactor surface zone (area of illumination) and produce fast cyclial movenent, strengthening fluid is in the mixing of reactor radial direction (being direction of illumination); And supply gas device or other power set.Described many group lamina of septum pellucidum can cause fluid to carry out the fast cycle cyclic motion within it, and strengthening fluid is in the mixing of reactor radial direction (being direction of illumination).
Should be understood that " trapezoidal " as herein described refers to the section from reactor, is trapezoidal by the wall of reactor and the adjacent formed zone of upper lower clapboard.
The present invention has strengthening fluid (algae liquid) in the mixing of direction of illumination, improve the dark cycle frequency of frustule light and frustule to the advantage that takes full advantage of of luminous energy, can effectively overcome the problem (for example the frustule of inside reactor can not fully be accepted degradation under illumination, the cell yield) that bioreactor occurs simultaneously when the light path direction is amplified.
Below specify implementation method of the present invention from different aspects.
1) composition of internals and structure
Internals adds baffle plate (the vertical plate of placing in reactor) by serial dividing plate (plate that horizontal tilt is placed in reactor) or dividing plate and forms.Dividing plate comprise the dividing plate that directly contact with the reactor wall and with the dividing plate of wall partition distance w2, preferred two kinds of dividing plates replace appearance.Length on each dividing plate horizontal direction preferably is no more than half of reactor width w.The angled α 0 of each dividing plate and reactor wall, every dividing plate and the formed angle [alpha] 0 of wall can be identical or different, but can form the zone of trapezoidal shape between the adjacent upper lower clapboard with the axis (with reference to the vertical dotted line of accompanying drawing 1 type b) of reactor wall and reactor, liquid in this special area of space (algae liquid) can form periodic circulation, thereby so that frustule is at central authorities' (inside reactor of reactor, be generally the dark space) and near-wall region (the reactor outside, be generally the light district) carry out the continuous circulation motion, thus so that frustule takes full advantage of the luminous energy of near-wall region.
In a specific embodiment, internals of the present invention is shown in Fig. 1 type b, comprise the dividing plate that contacts with wall and with the partition with certain angle of two each partition distance w2 of wall, so that between the adjacent upper lower clapboard with reactor wall, and form the zone of trapezoidal shape with the axis of reactor.Can be provided with many group slots in the two side of bioreactor (width), by dividing plate is inserted in the slot with stationary barrier; Also can make independently combined member body (all dividing plates and baffle combination are in the same place, make an integrated member that can split with body skin, can directly the integrated member that makes up be inserted in the housing during use).Can take out easily dividing plate like this or take out whole internals, with convenient thoroughly clear to reactor and dividing plate.In order totally to mix in the enhancing reactor, internals can suitably be adjusted, and makes it into part dividing plate by whole dividing plates and baffle plate forms, by forming runner between baffle plate and wall or baffle plate and the baffle plate, so that fluid has whole circulation, improve the whole mixed effect in the reactor.
The example of the exemplary internals that contains baffle plate can be shown in Fig. 1 type a and c.Comprise a baffle plate among the type a, the part dividing plate contacts with reactor wall, and the part dividing plate contacts with baffle plate, and this two classes dividing plate forms certain angle α 0 with reactor wall and baffle plate separately, α 0 can be identical or different, but form a trapezoid area between adjacent upper lower clapboard and reactor wall and the baffle plate; In addition, certain distance w2 ' (on the horizontal direction) is arranged between the dividing plate that contacts with the reactor wall and the plate washer, between the dividing plate that contacts with baffle plate and the reactor wall certain distance w2 is arranged, w2 ' and w2 can be identical or different.Preferred each dividing plate length in the horizontal direction is no more than half of reactor width w.More preferably, the length on the dividing plate horizontal direction adds that w2 or w2 ' are equal to or less than half of reactor width w.
Usually, when the internals that contains baffle plate places in the reactor, place it on the axis of reactor.
In accompanying drawing 1 type c, in reactor, place two baffle plates, the part dividing plate contacts with reactor wall, the part dividing plate contacts with second baffle with the first baffle plate respectively, this two classes dividing plate forms certain angle α 0 with reactor wall and corresponding baffle plate separately, α 0 can be identical or different, but form a trapezoid area between adjacent upper lower clapboard and reactor wall and the corresponding baffle plate.Distance between two baffle plates can be by the technician according to practical condition optimization.Should be understood that this moment on each dividing plate horizontal direction length and the distance between dividing plate and reactor wall and the baffle plate all should appropriately adjust.
The shape of dividing plate can change according to the type of reactor.For example, for flat-plate reactor, dividing plate can be square.For pillar or pipeline reactor, dividing plate can be fan-shaped, and that side that so contacts with reactor shell can be the arc that matches with housing.
For the pillar that contains baffle plate or pipeline reactor, baffle plate itself can be square, also can be columniform.When baffle plate when being square, that side that dividing plate contacts with baffle plate also should be square, and an other side then can be arc.When baffle plate when being cylindrical, dividing plate can be fan-shaped.
Distance h 1 along (type a and c) from reactor bottom under edge (type b) and the baffle plate under the dividing plate, distance h 2 along (type a and c) from the bottom under edge (type b) and the dividing plate on the dividing plate, distance h 3 between the dividing plate, distance h 4 along (type a and c) from reactor head on dividing plate (type b) and the baffle plate, the spacing of the horizontal direction between two dividing plates (type b) w1, the spacing of dividing plate and reactor wall (type a and c) w2, distance between two baffle plates (type c) w3, the structural parameter such as the crossing angle [alpha] 0 of dividing plate and wall all can be optimized adjustment, to obtain the size of optimum structure.Those skilled in the art also can be according to dividing plate and/or the baffle plate of the size Selection proper amt of bioreactor, to form the trapezoid area of proper amt in this bioreactor.
In a specific embodiment, take the flat bioreactor of 15L as example, the length of reactor is 25cm, and width w is 15cm, and height h is 40cm.The scope of h1 is 1～20cm, and the scope of h2 is 1～20cm, and the scope of h3 is 1～20cm, and the scope of h4 is 1～10cm, and the scope of w1 is 0.5～10cm, and the scope of w2 is 0.5～6cm, and the scope of w3 is 1～12cm, and the angle of α 0 is 1～85 °.In a preferred embodiment, the scope of h1 is 1～10cm, and the scope of h2 is 1～15cm, the scope of h3 is 1～8cm, and the scope of h4 is 1～8cm, and the scope of w1 is 0.5～5cm, the scope of w2 is 0.5～4cm, and the scope of w3 is 1～6cm, and the angle of α 0 is 45～85 °.
In addition, form the dead band in order to prevent the angle zone between dividing plate and the shell of reactor, can have less slit or aperture between dividing plate and the shell of reactor, flow so that fluid can form in the angle zone.Simultaneously, dividing plate and baffle plate all are that transparent material is made, and thinner thickness, to reduce Optical Absorption and to block.In addition, dividing plate and baffle plate can separate with the housing of outside, can independently take apart, take out from housing, in order to reactor and internals are cleaned.
2) reactor body structure and material
The agent structure of bioreactor (housing) can be flat, pillar or duct type.Can suitably adjust internals according to the outer hull shape.Because flat bioreactor is other closed photo bioreactor relatively, have and amplify relatively easy advantage, therefore, internals of the present invention at first is applied in the flat bioreactor.The outer transparent material of bioreactor housing is made, and can be the hard materials such as synthetic glass, unorganic glass (simple glass toughened glass etc.), also can be the flexible materials such as plastics film.If the employing flexible material then need to be installed the supporting structures such as wire netting in the reactor outside.
3) built-in source of artificial light in baffle plate and the dividing plate cavity
Baffle plate and dividing plate can be made the hollow cavity form, and like this, baffle plate and dividing plate not only play affects fluid flowing in reactor, promotes fluid outside the mixing of direction of illumination, can also be as the place of source of artificial light placement.The baffle plate of hollow cavity form and dividing plate, can increase on the one hand the light application ratio surface-area of reactor, another aspect can avoid source of artificial light to contact with the direct of algae liquid, and many problems of bringing to evade light source directly to contact with liquid are attached to light source surface etc. such as frustule; Simultaneously, source of artificial light places within the cavity, so that artificial lighting is fully absorbed by the frustule in the reactor.
4) power set and detection system
When the housing form of bioreactor is flat or column type, by ventilating mode so that in the reactor algae liquid mix, and when the housing of reactor is duct type, generally by pumping unit or air lift type system so that algae liquid in pipeline, flow.In addition, can in bioreactor, dispose various inspection and control systems, such as temperature detection and Controlling System, pH detection and feedback control system, dissolved oxygen detection system and light intensity detection system.
Further specify the structure of the internal mix member of novelty of the present invention and bioreactor and internal mix situation thereof the fluid whirlpool of direction of illumination uniqueness (especially) below in conjunction with accompanying drawing
Fig. 1 be the internal mix component application in flat bioreactor, the novel flat-plate reactor (structural representation, side-view) of series of formation.Fig. 2 is the pictorial diagram of the dull and stereotyped reactor of novel internal structure and control reactor.The internals of type a reactor is comprised of central baffle plate and tilt clapboard, the reactor left side is decline passway, has added the tilt clapboard that staggers up and down and place in the passage, can promote algae liquid in the mixing of direction of illumination, the right is the fluid rising passway simultaneously, and whole reactor can be realized overall flow.The built-in tilt clapboard of internals in the type b reactor for staggering up and down and placing forms trapezoid area in the vertical direction between dividing plate and the dividing plate; Simultaneously, the reactor left side is symmetrically distributed substantially with the right side.Type c reactor is comprised of baffle plate and tilt clapboard equally, formed the rising passway of fluid in reactor central authorities by two baffle plates, being two fluid decline passwaies near the reactor wall both sides, be mounted with the tilt clapboard that staggers and place in it, to promote the mixing of algae liquid on direction of illumination.
Fig. 3 is the Flow Field Distribution (calculate acquisition by CFD software for calculation Ansys12.0CFX, be under the 1.0vvm condition at air flow) in all types of flat bioreactors.
Type a reactor has 1 overall flow circulation, and has thereon with miniature fluid circulation the same among the reactor b.Interact between the systemic circulation of fluid and many partial circulatings, mix mutually.Liquid in the type a reactor mainly is to flow vertical (axially) at left passage, owing to produced Fluid Circulation, the radial flow of liquid and mixing are more obvious at the right passage of reactor.Type a forms (the many groups dividing plate that has increased horizontal tilt at the decline passway of single dividing plate airlift reactor) on single dividing plate airlift reactor basis.By 3 figure as seen, single dividing plate airlift reactor only exists the circulation of overall flow, and fluid flow is mainly take axial direction due as main, radially (direction of illumination) flow very a little less than, be unfavorable for the light autophyting growth of little algae.
Type b reactor is separated into a lot of special " cells " (two dividing plate between area of space) by internal partition, gas since buoyancy substantially along tilt clapboard up flow (flow deflector of the similar gas flow of internal partition), under the drive of gas, the fluid (referring to liquid) in each " cell " can produce fast cyclial movenent between reactor center zone (light dark space) and reactor surface zone (area of illumination).Between two " cells " of same layer, do not have dividing plate, therefore, between two Fluid Circulations of same layer, interact and mutually blending, so that two circulations are not rule very.Liquid in type b reactor mainly in cell separately cyclial movenent be main, and radially (illumination) direction is mobile fairly obvious, be conducive to frustule in the dark space and fully accept and utilize light in the light district, improve the optical energy utilization efficiency of frustule.Type b reactor can be regarded (having added the many groups of dividing plates that stagger and place and become horizontal tilt in bubbling style reactor) that forms on the basis of bubbling style reactor as, as seen from Figure 3, the dull and stereotyped reactor of control group (is bubbling style, without internals) interior two systemic circulations of fluid formation, fluid only forms flowing of radial direction (being direction of illumination) in top and the bottom of reactor to flow as main vertically.
Type c reactor has the flow circuit of two integral body, and has had thereon partial circulating many.This reactor is in rising passway, and liquid is axial flow, in the decline passway of both sides axially and radial motion all stronger.Type c forms (the many groups dividing plate that has increased horizontal tilt in the decline passway both sides of airlift reactor) on two dividing plate airlift reactors basis.Fig. 3 is arranged equally as seen, two dividing plate airlift reactors exist the circulation of two overall flow, and fluid flow is also mainly take axial direction due as main, radially (direction of illumination) flow a little less than, be unfavorable for the light autophyting growth of little algae.
This shows, liquid in control group reactor (bubbling style), single dividing plate and the two dividing plate airlift reactor is all take axial flow as main, liquid in the type b reactor is take radial flow as main, and the axial and radial flow of liquid is all apparent in view in type a and the c reactor.
In order to describe the advantage place of new internal member of the present invention and bioreactor thereof in detail, the below is specifically described with an example:
Embodiment 1: chlorella light autotrophy culturing process relatively in novel flat-plate reactor and the control reactor
In the flat bioreactor of 3 different internals and 1 control reactor, be respectively charged into the 14L tap water, add 2ml chlorine bleach liquor (available chlorine content 10%), behind the ventilation mixing, processed 12 hours, add afterwards in the Sulfothiorine and remaining clorox, check through potassium iodide starch test paper, determine without (general Sulfothiorine is excessive) behind the clorox, after continuing the excessive Sulfothiorine of ventilation for some time oxidation, then in reactor, put into little algae light autotrophy substratum (f/2 substratum), through fully accessing the Chlorella pyrenoidesa seed behind the mixing, the light autotrophy is cultivated beginning again.Initial algae cell density is about 0.15g/L, air flow 1.0vvm, and all there is illumination dull and stereotyped reactor both sides, and every sidelight is 10klx according to intensity.
As seen from Figure 4, the growing state of frustule is the poorest in the control reactor (without internals), and the highest algae cell density only is 0.916g/L.It is good to add in 3 kinds of reactors of different internals the equal contrast reactor of frustule growing state, wherein the high-cell density of little algae is 1.311g/L in the type b reactor, the high-cell density of little algae in the type a reactor is 1.189g/L, and the high-cell density of little algae is 1.241g/L in the type c reactor.Simultaneously, the specific growth rate of frustule in the exponential growth stage in 4 dull and stereotyped reactors of difference calculated, the result is that the specific growth rate of frustule in the control reactor is 0.051h
-1, the specific growth rate of frustule is 0.0619h in the type b reactor
-1, the specific growth rate of frustule is 0.0574h in the type a reactor
-1, the growth velocity of frustule is 0.0604h in the type c reactor
-1By above data as seen, add the high-density of frustule in the dull and stereotyped reactor of new internal member and growth velocity apparently higher than common flat bioreactor (bubbling style does not add any internals).This shows that the novel flat-plate reactor behind the new internal member of the present invention's design and the interpolation internals has good culture effect.
Although should be understood that in the mode of specific embodiment and set forth the present invention, in the situation that does not depart from spirit and scope of the invention, can make suitable change to the present invention, these are changed all within protection scope of the present invention.
Claims (10)
1. a bioreactor is characterized in that, described bioreactor comprises:
Transparent outer hull; With
Internals, described internals is included in and axially is mounted with the regularly arranged lamina of septum pellucidum that is horizontal tilt of polylith in the described housing, the angle that described dividing plate and shell wall side form is so that form the local space zone of trapezoidal shape between neighbouring dividing plate and the housing, the arrangement of described lamina of septum pellucidum makes between the fluid in the fluid in reactor center zone and reactor surface zone and produces fast cyclial movenent.
2. bioreactor as claimed in claim 1 is characterized in that, described transparent outer hull is flat, column type, duct type and other shape.
3. bioreactor as claimed in claim 1 is characterized in that, described dividing plate is hollowed out the formation hollow cavity.
4. bioreactor as claimed in claim 1 is characterized in that, described device of air or other power set of giving is air pump and gas distributor or other relevant power-equipment that can cause algae liquid to flow in bioreactor.
5. bioreactor as claimed in claim 2 is characterized in that, described transparent shell is by the transparent material with certain physical strength; Or made by flexible material.
6. bioreactor as claimed in claim 3 is characterized in that, if dividing plate is the transparent solid plate, then described dividing plate can split with shell of reactor, and dividing plate can freely be inserted in the reactor; If dividing plate is the cavity body dividing plate, then described dividing plate and shell of reactor are integrated.
7. bioreactor as claimed in claim 3 is characterized in that, and is described by placing source of artificial light in the cavate hollow cavity of dividing plate.
8. bioreactor as claimed in claim 1, it is characterized in that described bioreactor also comprises supply gas device or other power set, temperature detection and Controlling System, pH detection and feedback control system, dissolved oxygen detection system and inner light intensity detection system.
9. a member that is used for the described bioreactor of claim 1 is characterized in that described member is the regularly arranged lamina of septum pellucidum that is horizontal tilt of polylith, forms the local space zone of trapezoidal shape between each dividing plate.
10. each described bioreactor of claim 1-8 or member claimed in claim 9 shine into capable cultivation or the application in the chemical reaction at light requirement.
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| CN 201210097707 CN103361257A (en) | 2012-04-05 | 2012-04-05 | Light direction hybrid reinforcement-based novel internal component and photobioreactor |
| PCT/CN2012/078265 WO2013149442A1 (en) | 2012-04-05 | 2012-07-06 | Novel internal component and photobioreactor based on enhanced mixing along light direction |
| CN201280072224.2A CN104640970B (en) | 2012-04-05 | 2012-07-06 | A kind of new internal component and bioreactor based on reinforcing direction of illumination mixing |
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| CN2197366Y (en) * | 1994-08-19 | 1995-05-17 | 李维仁 | Photo-biologic reactor |
| CN2319413Y (en) * | 1998-01-19 | 1999-05-19 | 华南理工大学 | Pipeline gas raising magnetic treatment optical biological reactor microalgae breeding appts. |
| CN100587054C (en) * | 2006-05-15 | 2010-02-03 | 华东理工大学 | Novel multi-section flat type photo-bioreactor |
| CN102260629B (en) * | 2010-05-28 | 2014-07-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
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2012
- 2012-04-05 CN CN 201210097707 patent/CN103361257A/en active Pending
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2013149442A1 (en) | 2013-10-10 |
| CN104640970A (en) | 2015-05-20 |
| CN104640970B (en) | 2017-04-05 |
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