CN102260629B - Plate-type photobioreactor - Google Patents
Plate-type photobioreactor Download PDFInfo
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- CN102260629B CN102260629B CN201010193837.4A CN201010193837A CN102260629B CN 102260629 B CN102260629 B CN 102260629B CN 201010193837 A CN201010193837 A CN 201010193837A CN 102260629 B CN102260629 B CN 102260629B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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Abstract
The invention relates to a plate-type photobioreactor, which comprises at least one flow channel, wherein each flow channel comprises at least 2 upper baffle plates, which are arranged on the inner wall face on a light receiving side, and at least 2 lower baffle plates, which are arranged on the inner wall face on a backlight side; and the included angles of the length directions of the upper and lower baffle plates and the flowing direction of culture solution are 20 to 70 DEG and are opposite. The plate-type photobioreactor has a specific internal structure, allow microalgae cells to move back and froth between the bright and dark areas in the photobioreactor, and give play to the 'flash effect' of the microalgae cells, thereby improving the light energy utilization and microalgae cells yield of the microalgae large-scale culture. The plate-type photobioreactor disclosed by the invention is suitable for large-scale culture of various microalgae cells.
Description
Technical field
The present invention relates to a kind of board-like bioreactor, specifically relate to a kind of board-like bioreactor with special internal structure.
Background technology
Microalgae cell is rich in the biological substance of the multiple high added values such as amino acid, protein, VITAMIN, unsaturated fatty acids, has become the important materials source of human food prods, medicine, dyestuff, field of fine chemical.At present, along with the exhaustion day by day of the fossil energy such as oil, coal, biorefinery based on biomass has caused people's great attention, micro-algae can provide a large amount of biomass (grease, starch, Mierocrystalline cellulose) as important renewable energy source, in biorefinery field, have broad application prospects.
Micro-algae large-scale is cultivated open cultivation and the two kinds of modes of closed cultivation of being mainly divided into.Open cultivation, be to develop the earliest and apply the most general a kind of mode, countries in the world, particularly China still set it as major way (Borowitzka L.T., the Bioresource Technology that micro-algae industrialization is cultivated at present, 1991,38:251～252).In open cultivation, representational reactor comprises racetrack cultivation pool, circular cultivation pool.The major advantage of this training method is that structure is simple, with low cost and easy and simple to handle.Shortcoming is that the efficiency of light energy utilization is low, is subject to the impact of external environment factor large, easily contaminated, and moisture evaporation is large.
Closed cultivation adopts the closed photo bioreactor of various ways (as pillar, tubular type and board-like) conventionally.Than open cultivation, closed cultivation is not easy contaminated, saving water resource, culture density is high, and results cost is low, and shortcoming is high (the Pulz O. of cost of investment, Applied Microbiology andBiotechnology, 2001,57:287～293; Lee Y-K., Journal of Applied Phycology, 2001,13:307～315; Tsygankov A.A., Applied Biochemistry and Microbiology, 2001,37 (4): 333～341).In order to overcome the high defect of closed cultivation cost, need on the one hand to reduce to cultivate required material, energy consumption cost, need on the other hand to increase substantially productive rate.Although the productive rate of closed cultivation will be higher than open cultivation, the efficiency of light energy utilization of micro-algae is also very low.Managing to improve frustule is the important channel of improving productive rate, reducing costs to the utilization ratio of luminous energy.
Because the photosynthesis process of micro-algae can be divided into two stages, be called photoresponse and dark reaction.At photoreaction stage, frustule is accepted photon and is converted to chemical energy; In cloudy, turbid phase, frustule utilizes chemical energy synthetic cell component, and in cloudy, turbid phase, frustule does not need illumination, and even illumination is harmful on the contrary.Therefore,, for single algae cell, lasting illumination means the waste of photon.
Scientists has been understood, in the bioreactor of cultivating in scale and under common cell density, and decay rapidly when light is propagated in nutrient solution, the penetration range of light is several millimeters, only has 1mm left and right under high-cell density.In bioreactor, in fact can be divided near plane of illumination inner-wall surface Guang district with outside dark space two portions.If frustule with CF (usually above the frequency of 1Hz) when bioreactor Guang district and the dark space frequent replacement, can produce " sparkle effect ", the utilization ratio of luminous energy (the Janssen M that can be greatly improved, Slenders P, Tramper J, et al., Enzyme MicrobialTechnology, 2001,29:298～305; Matthijs H.C.P, Balke H, Mur L.R, et al., Biotechnology and Bioengineering, 1996,50:98～107).; when frustule shuttles between bioreactor Guang district and dark space; the frustule that just can make to accept illumination enters in time dark space and carries out dark reaction; make the frustule of dark reaction get back to light district simultaneously and again accepted illumination, so just the photon that enters bioreactor has been fully utilized.Therefore by the mixing of hot housing liquid, high frequency between frustule Guang district and dark space is shuttled, can improve the overall efficiency of light energy utilization and the output of frustule.
But in traditional board-like bioreactor, the mobile mixing of nutrient solution is still undesirable.Between 1～10cm not etc., the inner-wall surface of these bioreactors is smooth often for the thickness of slab of board-like bioreactor (comprising horizontal positioned, slant setting, vertical place etc.).Be subject to the restriction of reactor wall, in close inner-wall surface place nutrient solution undercompounding, frustule is very little perpendicular to the speed of flow direction (hereinafter referred to as optical path direction), be difficult to be created in shuttling back and forth between light district and dark space, frustule can produce light inhibition or even light injury (being that illumination is excessive) near being positioned at for a long time plane of illumination inner-wall surface; And apart from plane of illumination inner-wall surface frustule far away for a long time in light restriction state (being illumination deficiency), make the efficiency of light energy utilization not high.
Design by certain internal structure can hot housing liquid mixing.As the disclosed box bioreactor of plate of putting hollow weir that adds in Chinese patent CN200610026539.X, mobile mixing that can hot housing liquid by this specific interior arrangement, meanwhile, inner light source can be installed in described hollow weir or as distribution device in gas-fluid.Although such device that installs hollow weir additional can play the mixing effect of hot housing liquid, complex structure, manufacturing cost is high, and the mixing of frustule is unordered mixing, is difficult to ensure that frustule is subject to the illumination of specific period.
Summary of the invention
The object of the invention is on the basis of existing board-like bioreactor, provide a kind of simple in structure, be convenient to the board-like bioreactor manufactured in batches, it has specific internal, can realize frustule shuttling between bioreactor Guang district and dark space, can bring into play " sparkle effect " of frustule, thereby improve the microalgae mass efficiency of light energy utilization of cultivating and the output that improves microalgae cell.Board-like bioreactor of the present invention is suitable for mass-producing and cultivates various micro-algaes.
Board-like bioreactor provided by the invention, comprises at least one runner, and wherein, each runner comprises:
-at least 2 overhead gages, are arranged on the inner-wall surface of plane of illumination;
-at least 2 lower baffle plates, are arranged on the inner-wall surface of matte surface;
Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and angle opposite direction.
In embodiments of the present invention, described board-like bioreactor is hardware bioreactor or software bioreactor.Thereby described hardware bioreactor comprises plane of illumination and the matte surface being made by hard plate material and separates along the bearing rib of nutrient solution flow direction setting the runner that Formation cross-section is rectangle.Described software bioreactor is directly with the position that the method such as hot pressing or bonding need arrange gusset at soft material (as coiled material or film material), plane of illumination and matte surface to be bonded together, thereby being filled with the softened Formation cross-section of runner after nutrient solution is circular or oval-shaped runner, and the effect of gusset is still played at bonded position.
In embodiments of the present invention, along in flow channel length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
In embodiments of the present invention, described upper and lower baffle plate is 30～100% of this board-like bioreactor width of flow path along the projecting length of width of flow path direction.
In embodiments of the present invention, the height of described upper and lower baffle plate (height in the cross section of baffle plate own) is 1mm～10mm.
In embodiments of the present invention, the cross section of described upper and lower baffle plate can be rectangle, trapezoidal, trilateral, semicircle or other shape; Wherein, the thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
In embodiments of the present invention, the material of described baffle plate can be light transmissive material or select the light-proof material that easily processing is installed.Described light transmissive material can be the one in glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.) etc.; Described light-proof material can be the one in plastics, rubber, resin etc.Preferably, the material of described baffle plate is identical with the material of plane of illumination.
Described plane of illumination is to be made by various light transmissive materials, as glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.).
In embodiments of the present invention, the length of described runner can be cultivated and need to do corresponding adjustment according to reality, and in parallel multiple runners or series connection can be used, and forms the board-like bioreactor of different profiles.Now gusset plays the effect of traverse baffle simultaneously.
When scale operation, can, according to the requirement of output, can or connect multiple board-like bioreactors parallel connections of the present invention, more supporting other device.
The board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated, can be built in outdoor outdoor or booth, make full use of natural light, can cultivate various micro-algaes, comprise spirulina, chlorella, salt algae, diatom, chrysophyceae etc.
Compared with prior art, the advantage of the board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated is: " sparkle effect " that can give full play to frustule, improve the efficiency of light energy utilization that microalgae mass is cultivated, improve the output of unit lighting area.In addition, the board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated is simple in structure, is convenient to manufacture in batches, and the number that can increase simply reactor expands the scale of production.
Brief description of the drawings
The skeleton view of one runner of Fig. 1, the board-like bioreactor of hardware of the present invention;
The right view of one runner of Fig. 2, the board-like bioreactor of hardware of the present invention;
The vertical view of the board-like bioreactor of Fig. 3, multiple runner parallel connections of the present invention;
The vertical view of the board-like bioreactor of Fig. 4, multiple runner series connection of the present invention;
The front view section of the baffle plate of Fig. 5, several different cross section shapes in the present invention's one runner;
The change in location of frustule in optical path direction in Fig. 6, board-like bioreactor of the present invention;
One cross section of Fig. 7, the board-like bioreactor of software of the present invention is the right view of circular runner;
The right view that one cross section of Fig. 8, the board-like bioreactor of software of the present invention is oval-shaped runner;
Fig. 9, microalgae culture system schematic diagram;
Wherein:
1. runner nutrient solution entrance 2. runner nutrient solutions export 3. plane of illuminations
4-1. overhead gage 4-2. lower baffle plate 5. matte surfaces
6. the 7. trapezoidal baffle plate of rectangle baffle plate 8. of gusset (traverse baffle)
9. trilateral baffle plate 10. fluid delivery system 11. bioreactors
12. heat exchanger 13. gas-liquid exchange device 14 end plates
15. bioreactor culture liquid entrance 16. bioreactor culture liquid outlets
α. the angle of nutrient solution flow direction in the length direction of overhead gage and runner
β. the angle of nutrient solution flow direction in the length direction of lower baffle plate and runner.
Embodiment
Board-like bioreactor provided by the invention, comprises at least one runner, and wherein, each runner comprises :-at least 2 overhead gage 4-1, are arranged on the inner-wall surface of plane of illumination 3;-at least 2 lower baffle plate 4-2, are arranged on the inner-wall surface of matte surface 5; Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and angle opposite direction.
One embodiment of the present invention, described board-like bioreactor is hardware bioreactor, as depicted in figs. 1 and 2, it comprise plane of illumination (top panel) 3 that made by hard plate material and matte surface (lower panel) 5 and along nutrient solution flow direction (i.e. the direction to runner nutrient solution outlet 2 by runner nutrient solution entrance 1) thus the bearing rib 6 arranging is separated the runner that Formation cross-section is rectangle.Wherein, each runner comprises: at least 2 overhead gage 4-1, are arranged on the inner-wall surface of plane of illumination 3; At least 2 lower baffle plate 4-2, are arranged on the inner-wall surface of matte surface 5; The size of the angle β of the size of angle α of the length direction of overhead gage and nutrient solution flow direction and the length direction of lower baffle plate and nutrient solution flow direction is 20～70 degree, and the opposite direction (as shown in Figure 3, Figure 4) of angle α and angle β.
Another embodiment of the present invention, described board-like bioreactor is software bioreactor, as shown in Figure 7 and Figure 8, it is directly bonded together plane of illumination 3 and matte surface 5 with the position that the method such as hot pressing or bonding need arrange gusset at soft material (as coiled material or film material), thereby being filled with the softened Formation cross-section of runner after nutrient solution is circular or oval-shaped runner, and the effect of gusset (traverse baffle) is still played at bonded position.Wherein, each runner comprises: at least 2 overhead gage 4-1, are arranged on the inner-wall surface of plane of illumination 3; At least 2 lower baffle plate 4-2, are arranged on the inner-wall surface of matte surface 5; The size of the angle β of the size of angle α of the length direction of overhead gage and nutrient solution flow direction and the length direction of lower baffle plate and nutrient solution flow direction is 20～70 degree, and the opposite direction (as shown in Figure 3, Figure 4) of angle α and angle β.
In embodiments of the present invention, along in flow channel length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
In embodiments of the present invention, as shown in Fig. 2,3,4,7,8, described upper and lower baffle plate is 30～100% of this board-like bioreactor width of flow path along the projecting length of width of flow path direction.
In embodiments of the present invention, the height of described upper and lower baffle plate (height in the cross section of baffle plate own) is 1mm～10mm.
In embodiments of the present invention, as shown in Figure 5, the cross section of described upper and lower baffle plate can be rectangle, trapezoidal, trilateral, semicircle or other shape; Wherein, the thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
In embodiments of the present invention, the material of described baffle plate can be light transmissive material or select the light-proof material that easily processing is installed.Described light transmissive material can be the one in glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.) etc.; Described light-proof material can be the one in plastics, rubber, resin etc.Preferably, the material of described baffle plate is identical with the material of plane of illumination.
Described plane of illumination is to be made by various light transmissive materials, as glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.).
In embodiments of the present invention, the spacing between plane of illumination and the matte surface of each runner in described hardware bioreactor is 10mm～100mm, and the width of runner is 10mm～300mm.
In embodiments of the present invention, plane of illumination and the matte surface both sides of each runner in described software bioreactor are bonding, and two bonding part spacings are 10mm～300mm.
In one embodiment of the present invention, the length of described runner can be cultivated and need to do corresponding adjustment according to reality, can make multiple runner parallel connections with the double traverse baffle that does of gusset, as shown in Figure 3; Or make multiple runner series connection, as shown in Figure 4.
Can select according to Drawing abillity manufacture and the mounting means of baffle plate.For example, the baffle plate of having manufactured separately can be bonded on the inner-wall surface of board-like bioreactor, with mould, the plane of illumination of board-like bioreactor 3 is one-body molded with baffle plate with matte surface 5 or the baffle plate of manufacturing in advance support is connected rearmountedly to enter to make baffle plate be close to the plane of illumination 3 of board-like bioreactor and the inner-wall surface of matte surface 5 in board-like bioreactor.
When scale operation, can, according to the requirement of output, can or connect multiple board-like bioreactors parallel connections of the present invention, more supporting other device.For example, as shown in Figure 9, cultivate in the process of frustule in actual production, the board-like bioreactor 11 of the efficiency of light energy utilization of cultivating except above-mentioned raising microalgae mass of the present invention in whole culture systems, also be furnished with gas-liquid exchange device 13 for air feed and carry out oxygen parsing, fluid delivery system 10, heat exchanger 12, coupling device, material distribution device, temperature monitoring control device, and other auxiliary equipment.
The principle of board-like bioreactor of the present invention is: in the time that nutrient solution flows through board-like bioreactor of the present invention with certain speed, can in runner, form spiral motion, and can between baffle plate, produce whirlpool, promote frustule and in optical path direction, form certain to-and-fro movement, thereby realize frustule shuttling in board-like bioreactor Guang district and dark space.By adjusting the flow velocity of nutrient solution, the size of baffle plate, the distance of baffle plate, can obtain the dark loop cycle of different light, and the frequency of the dark circulation of light can be higher than 1Hz.What Fig. 6 represented is in the board-like photoreactor of a hardware, the spacing that the plane of illumination 3 of runner becomes miter angle with the length direction of matte surface 5 internal surface spacing 16mm, overhead gage and lower baffle plate with nutrient solution flow direction and is full of width of flow path, baffle plate is that 80mm, height of baffle plate are that 4mm, Weir Plate Thickness are while being 2mm, when the particle that represents frustule moves in reactor, its position in optical path direction over time.Can be clear that, particle is fluctuation regularly in optical path direction, and a dark loop cycle of complete light is about 160ms.In the time that the nutrient solution flow velocity in board-like bioreactor is 10cm/s～100cm/s, within the scope of this, can form the dark loop cycle of light of 500ms～20ms, the corresponding dark cycle frequency of light is 2Hz～50Hz.
The board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated, can be built in outdoor outdoor or booth, make full use of natural light, can cultivate various micro-algaes, comprise spirulina, chlorella, salt algae, diatom, chrysophyceae etc.
Embodiment 1
Under shining, outdoor natural light carries out the cultivation of spirulina.The board-like bioreactor horizontal positioned of the culture systems building, manufacture material is poly (methyl methacrylate) plate, and the thickness of upper and lower panel is 2mm, and the thickness of end plates 14 and gusset 6 is 2mm, and the length of gusset 6 is 80cm.Board-like bioreactor is of a size of: long 1m, wide 1.022m, upper and lower panel internal surface spacing 16mm, it is respectively the wide runner of 10cm that dull and stereotyped inside is divided into 10 by gusset, each runner parallel connection (as Fig. 3).Be close to the square-section baffle plate 3 that top panel and the stickup of lower panel inner side are prepared by poly (methyl methacrylate) plate material, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 45 degree, and angle opposite direction, baffle length 14.1cm (baffle plate along the projecting length of nutrient solution width of flow path direction be width of flow path 100%), be 80mm along the spacing of flow channel length direction overhead gage, height of baffle plate is 4mm, and Weir Plate Thickness is 2mm.10 overhead gages and 10 lower baffle plates (region between baffle plate cover approximately 82% that the efficient light of bioreactor shows up) are installed in each runner.
Culture systems (is divided into two groups by the above-mentioned board-like bioreactor 11 of 10 series connection, 5 of every group of series connection), fluid delivery system 10 (commercially available surge pump), heat exchanger 12, gas-liquid exchange device 13 form, mode of connection as shown in Figure 9, wherein board-like bioreactor 11 horizontal positioned.The flow velocity of nutrient solution in reactor is 20cm/s.
Algae kind is from Chinese Academy Of Sciences Process Engineering Research Institute, and kind is spirulina plalensis, and substratum is Zarrouk substratum, and in nutrient solution, the starting point concentration of sodium bicarbonate is 0.1mol/L.
Before cultivation, use the water after 0.2 μ micro-filtrate membrane filtration to clean above-mentioned bioreactor.According to Zarrouk culture medium prescription preparation 160L substratum, 0.2 μ micro-filtrate membrane filtration for substratum.Preparation kind of liquid according to a conventional method, inoculum density 0.1g/L.Medium pH value is set in 9.0, and 25～35 DEG C of envrionment temperatures, adopt outdoor natural lighting, and controlling culture temperature is 32 ± 1 DEG C.Period sampling measuring algae cell density.In the time that algae cell density reaches 3g (dry weight)/L, start semicontinuous gathering, collecting method is that draw 20% of nutrient solution in culture systems (approximately every 3 days), and the filtered liquid after filtering is returned in former culture systems.Washing, dry after the frustule results that filtration obtains.
Between incubation period, regularly detect the concentration of other nutritive salt and supplement in time, and supplementing a small amount of water to make up evaporation of water loss.Between incubation period, to the mixed gas that passes into air and carbonic acid gas in the each gas-liquid exchange device in culture systems, the flow of mixed gas is 0.6L/L.min, and the molar fraction of carbon dioxide in gas mixture is 3%.Culturing process continues 30 days, and the area productive rate of spirulina is about 36g/m
2.d.
Comparative example 1
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and difference is without baffle plate in board-like bioreactor.
Culturing process continues 30 days, and the area productive rate of spirulina is 25g/m
2.d.
Obviously, under same culture condition, area productive rate in the board-like bioreactor of baffle plate of the embodiment of the present invention 1 higher than comparative example 1 without the area productive rate in the board-like optical-biological reaction of baffle plate, the board-like bioreactor of baffle plate of the present invention improves obviously the efficiency of light energy utilization of frustule.
Embodiment 2
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, difference is that the height of put into baffle plate is 2mm, thickness is 1mm, be 30mm along the spacing of flow channel length direction overhead gage, the corner dimension of the length direction of baffle plate and nutrient solution flow direction is 70 degree, baffle plate is 50% of width of flow path along the projecting length of nutrient solution width of flow path direction, and 25 overhead gages and 25 lower baffle plates are installed in each runner, the region between baffle plate cover that the efficient light of bioreactor shows up approximately 74%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 34g/m
2.d.Higher than comparative example 1 area productive rate (25g/mw under same culture condition in without the board-like bioreactor of baffle plate
2.q) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 3
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and difference is that the height of put into baffle plate is 6mm, and thickness is 3mm, is 100mm along the spacing of flow channel length direction overhead gage, and the length direction of baffle plate becomes 20 degree angles with nutrient solution flow direction.6 overhead gages and 6 lower baffle plates are installed in each runner, the region between baffle plate cover that the efficient light of bioreactor shows up approximately 78%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 33g/m
2.d.Higher than comparative example 1 area productive rate (25g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 4
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, difference is to be 15mm along the spacing of flow channel length direction overhead gage, baffle plate is 30% of width of flow path along the projecting length of nutrient solution width of flow path direction, 50 overhead gages and 50 lower baffle plates are installed in each runner, the region between baffle plate cover that the efficient light of bioreactor shows up approximately 77%)
Culturing process continues 30 days, and the area productive rate of spirulina is about 34g/m
2.d.Higher than comparative example 1 area productive rate (25g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 5
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and difference is that nutrient solution entrance flow velocity is 50cm/s.
Culturing process continues 30 days, and the area productive rate of spirulina is about 39g/m
2.d.Higher than comparative example 1 area productive rate (25g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 6
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and difference is that nutrient solution entrance flow velocity is 10cm/s.
Culturing process continues 30 days, and the area productive rate of spirulina is about 32g/m
2.d.Higher than comparative example 1 area productive rate (25g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 7
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, difference is, the upper and lower panel internal surface spacing 30mm of board-like bioreactor, the height of the baffle plate of putting into is 10mm, thickness is 5mm, is 120mm along the spacing of flow channel length direction overhead gage, and the length direction of overhead gage becomes 60 degree angles with nutrient solution flow direction, the length direction of lower baffle plate becomes 30 degree angles with nutrient solution flow direction, the vergence direction of the length direction of overhead gage and lower baffle plate compared with flow channel length direction is contrary.7 overhead gages and 7 lower baffle plates are installed in each runner, the region between baffle plate cover that the efficient light of bioreactor shows up approximately 78%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 33g/m
2.d.Higher than comparative example 2 area productive rate (23g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Comparative example 2
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 7, and difference is without baffle plate in board-like bioreactor.
Culturing process continues 30 days, and the area productive rate of spirulina is 23g/m
2.d.
Embodiment 8
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and difference is, the upper and lower panel internal surface spacing 30mm of board-like bioreactor, and the cross-sectional shape of the baffle plate of putting into is trilateral, and the height of baffle plate is 8mm, and the thickness of half eminence is 3mm.
Culturing process continues 30 days, and the area productive rate of spirulina is about 32g/m
2.d.Higher than comparative example 2 area productive rate (23g/m under same culture condition in without the board-like optical-biological reaction of baffle plate
2.d) efficiency of light energy utilization that, shows frustule improves obviously.
Embodiment 9
Under shining, outdoor natural light carries out the cultivation of spirulina.Other condition is with embodiment 1, and difference is that board-like bioreactor (as Fig. 3) is the board-like bioreactor of software of being made up of polyethylene film, and polyethylene film thickness is 0.2mm.In the position that gusset is set as shown in Figure 3, plane of illumination and matte surface are bonded together by the method for hot pressing, the length of plate-type reactor is 1m, and width is 1.022m, by bonding, board-like bioreactor is divided into 10 runners that 10cm is wide.Form inwardly protruding (baffle plate 4) in the mode of outside hot pressing in plane of illumination and the matte surface inner side of each runner by built-in mould simultaneously, the cross-sectional shape of projection is trilateral (as 9 in Fig. 5), the height 4mm of projection, halfwidth degree 2mm, the projection length direction of (baffle plate) and the corner dimension of nutrient solution flow direction are 45 degree, and the protruding length direction of plane of illumination and matte surface and the angle opposite direction of nutrient solution flow direction, projection is 10cm along the projecting length of nutrient solution width of flow path direction, be 80mm along the spacing of flow channel length direction projection (baffle plate).Plane of illumination and matte surface inner side respectively arrange 10 projections (baffle plate) in each runner, the region between projection cover that the efficient light of bioreactor shows up approximately 82%.
When actual cultivation, when nutrient solution is pumped into board-like bioreactor, each runner is softened is about the elliptical shape (as Fig. 8) that 8cm, short diameter (height) are about 3.6cm for long diameter (wide).
The culture volume of preparation is about 170L, and control condition and collecting method are with embodiment 1, and the density while gathering is about 5g/L.
Between incubation period, regularly detect the concentration of other nutritive salt and supplement in time, and supplementing a small amount of water to make up evaporation of water loss.Between incubation period, to the mixed gas that passes into air and carbonic acid gas in the each gas-liquid exchange device in culture systems, the flow of mixed gas is 1.0L/L.min, and the molar fraction of carbon dioxide in gas mixture is 3%.Culturing process continues 30 days, and the area productive rate of spirulina is about 38g/m
2.d.
Comparative example 3
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 9, and difference is in board-like bioreactor, there is no projection (baffle plate).
Culturing process continues 30 days, and the area productive rate of spirulina is about 26g/m
2.d.
Obviously, under same culture condition, area productive rate in the board-like bioreactor of baffle plate of the embodiment of the present invention 9 higher than comparative example 3 without the area productive rate in the board-like optical-biological reaction of baffle plate, the board-like bioreactor of baffle plate of the present invention improves obviously the efficiency of light energy utilization of frustule.
Claims (11)
1. a board-like bioreactor, comprises at least one runner, it is characterized in that, each runner comprises:
-at least 2 overhead gages, are arranged on the inner-wall surface of plane of illumination;
-at least 2 lower baffle plates, are arranged on the inner-wall surface of matte surface;
Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and angle opposite direction;
In the time that nutrient solution flows through described board-like bioreactor, can in runner, form spiral motion, and can between baffle plate, produce whirlpool, promote microalgae cell and in optical path direction, form certain to-and-fro movement, thereby realize microalgae cell shuttling in board-like bioreactor Guang district and dark space.
2. board-like bioreactor according to claim 1, wherein, described board-like bioreactor is hardware bioreactor or software bioreactor.
3. board-like bioreactor according to claim 2, wherein, thus described hardware bioreactor comprise the plane of illumination that made by hard plate material and matte surface and separate along the bearing rib of nutrient solution flow direction setting the runner that Formation cross-section is rectangle.
4. board-like bioreactor according to claim 2, wherein, described software bioreactor is that the position that directly at soft material gusset need be set is bonded together plane of illumination and matte surface, is circular or oval-shaped runner thereby be filled with the softened Formation cross-section of runner after nutrient solution.
5. board-like bioreactor according to claim 1, wherein, along in flow channel length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
6. board-like bioreactor according to claim 1 or 5, wherein, described upper and lower baffle plate is 30～100% of this board-like bioreactor width of flow path along the projecting length of width of flow path direction.
7. board-like bioreactor according to claim 1 or 5, wherein, the height of described upper and lower baffle plate is 1mm～10mm.
8. board-like bioreactor according to claim 1 or 5, wherein, the cross section of described upper and lower baffle plate is rectangle, trapezoidal, trilateral, semicircle; The thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
9. board-like bioreactor according to claim 1 or 5, wherein, the material of described baffle plate is transparent glass, synthetic glass, plastics.
10. board-like bioreactor according to claim 1 or 5, wherein, the material of described baffle plate is lighttight plastics, rubber, resin.
11. board-like bioreactors according to claim 1 or 5, wherein, the in parallel or series connection of described runner is used.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103361257A (en) * | 2012-04-05 | 2013-10-23 | 华东理工大学 | Light direction hybrid reinforcement-based novel internal component and photobioreactor |
| CN103911274A (en) * | 2012-11-30 | 2014-07-09 | 新奥科技发展有限公司 | Device and method for large-scale culture of microalgae |
| CN103421677B (en) * | 2013-04-26 | 2018-08-07 | 中国科学院过程工程研究所 | A kind of baffle for microalgae large-scale culture opens pond |
| CN104293673B (en) * | 2013-07-19 | 2017-05-24 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293674B (en) * | 2013-07-19 | 2017-05-24 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293675B (en) * | 2013-07-19 | 2017-07-25 | 中国石油化工股份有限公司 | A kind of method of pilot scale culture microalgae |
| CN104789451B (en) * | 2014-01-17 | 2017-01-04 | 中国石油化工股份有限公司 | A kind of photosynthetic organism culture apparatus |
| CN104789442B (en) * | 2014-01-17 | 2017-04-26 | 中国石油化工股份有限公司 | Method for culturing photosynthetic organisms |
| CN105219616B (en) * | 2014-06-17 | 2017-11-21 | 中国科学院过程工程研究所 | A kind of board-like bioreactor of software |
| CN104293664B (en) * | 2014-08-07 | 2016-12-07 | 中国航天员科研训练中心 | The applied stream in space controlled locked cell culture chamber |
| CN105733924A (en) * | 2014-12-12 | 2016-07-06 | 国家开发投资公司 | Thin-layer horizontal-type photo-biological reactor and photo-biological culture system |
| CN105002086B (en) * | 2015-07-16 | 2017-12-01 | 中国海洋大学 | A kind of raceway pond microalgae cultivating system for continuing aerating collecting frustule using microbubble |
| CN105018331B (en) * | 2015-07-16 | 2017-07-21 | 中国海洋大学 | A kind of multidirectional turbulent flow, the raceway pond microalgae culture system of mixing |
| EP3670642A4 (en) * | 2017-08-16 | 2021-05-19 | Nippon Soda Co., Ltd. | Microorganism culture system |
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