CN105219616B - A kind of board-like bioreactor of software - Google Patents
A kind of board-like bioreactor of software Download PDFInfo
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- CN105219616B CN105219616B CN201410270445.1A CN201410270445A CN105219616B CN 105219616 B CN105219616 B CN 105219616B CN 201410270445 A CN201410270445 A CN 201410270445A CN 105219616 B CN105219616 B CN 105219616B
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Abstract
The present invention relates to microdisk electrode photobioreactor field.The board-like bioreactor of software of the present invention, including runner, also include bioreactor culture liquid entrance (1), bioreactor culture liquid outlet (2), host film (5), gusset (6) and drawstring (7), separated between runner by gusset (6)；Bioreactor culture liquid entrance (1) forms bioreactor culture liquid entrance cavity (10) with runner nutrient solution entrance (8), and bioreactor culture liquid outlet (2) forms bioreactor culture liquid outlet plenum (11) with runner nutrient solution outlet (9)；A drawstring (7) is provided with the bioreactor culture liquid entrance cavity (10) and/or bioreactor culture liquid outlet plenum (11).Present invention optimizes the overall structure of bioreactor；Improve the service life of the board-like bioreactor of software；Bioreactor is reduced because the reasons such as aging, stress cause the danger of rupture.
Description
Technical field
The present invention relates to microdisk electrode photobioreactor field, in particular it relates to a kind of internal enhancing, multithread
The road board-like bioreactor of software in parallel.
Background technology
With the increasingly depleted of fossil energy and the getting worse of environmental pollution, people begin look for new alternative energy source.
Luminous energy can be effectively utilized by H as one kind2O、CO2The photosynthetic autotrophs unicellular organism of organic resource is converted into inorganic salts,
Microalgae has the advantages of fat content is high, growth cycle is short, yield is high, worldwide of increased attention,
It is considered as a kind of most promising bioenergy raw material.In addition, microalgae also contains protein, carbon hydrate necessary to the mankind
Thing, polyunsaturated fatty acid and other bioactive substances.
The precondition of microalgae industry development is to carry out pilot scale culture, so as to obtain the biomass material of abundance.Microalgae
Large-scale culture can be divided into open and closed.Open microdisk electrode refers to various open ponds, by years development,
Applied in commercialization microalgae large-scale culture (Borowitzka L.T., Bioresource Technology, 1991,38:
251-252), it has the characteristics that technology is simple, low investment, is taken in the large-scale culture of spirulina, chlorella and salt algae
Obtained good effect.But there is unstable condition of culture, unit volume low yield in it, the efficiency of light energy utilization is low, is not easy to protect simultaneously
The shortcomings of holding purebred culture.
Closed culture refers to take various forms the closed photo bioreactor of (such as pillar, tubular type and board-like).With
Open culture is compared, and closed culture is not susceptible to pollution, saving water resource, culture density is high, harvest cost is low, and shortcoming is
Cost of investment it is high (Pulz O., Applied Microbiology and Biotechnology, 2001,57:287-293；Lee
Y-K.,Journal of Applied Phycology,2001,13:307-315；Tsygankov A.A.,Applied
Biochemistry and Microbiology,2001,37(4):333-341).In order to overcome closed toxigenic capacity high
Defect, on the one hand need to design by optimizing bioreactor, the efficiency of light energy utilization is improved, so as to increase substantially yield；Separately
On the one hand then need to reduce the cost of investment of bioreactor and cultivate required material, energy consumption cost.
Traditional bioreactor is more using the manufacture of the materials such as glass or stainless steel, their stability and durability
Height, but cost is too high, it is difficult to large-scale application.Therefore, low cost optical bioreactor causes people and more and more paid attention to,
And more and more applied to microalgae large-scale culture (Tredici M.R.et al, Biotechnol Bioeng,
2009,102,100-112.；Bergmann P.et al,Chem Ing Tech,2013,85,202-205；Willson,B.,
2009,doi:10.1088/1755-1307/6/9/192015；Yoo J.J.et al,Bioprocess Biosyst Eng,
2013,36,729-736).This kind of bioreactor is made of software light transmission film material more, is easy to batch micro operations, is built
Construction is simple, compared with traditional bioreactor, cost is greatly lowered.
The optimization of bioreactor then mainly by the mixing of hot housing liquid, make frustule light area and dark space it
Between high-frequency shuttle, flash effect is realized, so as to improve the yield of the efficiency of light energy utilization of bioreactor and frustule.
Flash effect is a kind of phenomenon of photoautotroph generally existing, i.e. when frustule is in the light area of bioreactor and dark space
Between shuttle, so that it may so that the frustule for receiving illumination timely enter dark space carry out dark reaction, while cause complete
The frustule of dark reaction returns to light area and receives illumination again, so allows for the light quantum into bioreactor by abundant profit
With.If frustule is with specific frequency (frequency for being usually above 1Hz) in the light area of bioreactor and dark space frequent replacement
When, it can produce " flash effect ", the utilization rate of luminous energy can be greatly improved (Janssen M, Slenders P, Tramper
J,et al.,Enzyme Microbial Technology,2001,29:298~305；Matthijs H.C.P,Balke H,
Mur L.R,et al.,Biotechnology and Bioengineering,1996,50:98-107；Xue S.Z,Su
Z.F,Cong W,Journal of Biotechnology,2011,151(3),271-277)。
Can be with the mixing of hot housing liquid, wherein being added in bioreactor quiet by the design of certain internal structure
State blender or baffle plate, it with the mixing of hot housing liquid, can also not only realize the flash effect of microalgae cell.It is Chinese special
Sharp CN201010193837.4 provides a kind of board-like bioreactor with specific internal, and it passes through biological anti-
The plane of illumination and dull surface for answering device inwall set baffle plate algae solution is twist flowed along nutrient solution flow direction, to realize algae
Cell shuttling between the light area of bioreactor and dark space, " flash effect " of frustule is played, so as to improve
The efficiency of light energy utilization of microalgae mass culture and the yield for improving microalgae cell.But such a light of multiple runners is made of soft material
During bioreactor, there is stress to concentrate position so that reactor is easily broken, and reduce bioreactor uses the longevity
Life, and apart from the distance difference of bioreactor culture liquid entrance, (such as CN201010193837.4 is said each runner nutrient solution entrance
Shown in bright book accompanying drawing 3), cause culture flow velocity and flow condition in each runner variant so that inside bioreactor
The runner efficiency of light energy utilization is relatively low, and the overall efficiency of light energy utilization of bioreactor and microalgae are thin during so as to have impact on microdisk electrode
The yield of born of the same parents.
The content of the invention
It is an object of the present invention in view of the above-mentioned problems, provide a kind of internal runner strengthening, multiple flow passages parallel connection, each
The board-like bioreactor of the consistent software of flow velocity, to solve the board-like photo-biological of software described in patent CN201010193837.4
It is easily broken existing for reactor, the problems such as flow velocity is variant is cultivated in different runners.
The board-like bioreactor overall structure of software of the present invention is as shown in figure 1, except possessing
Outside board-like bioreactor feature described in CN201010193837.4, i.e. a runner or at least two it is parallel with one another
Runner, each runner include：
- at least 2 pieces of overhead gages 3, are arranged on the internal face of plane of illumination；
- at least 2 pieces of lower baffle plates 4, are arranged on the internal face of dull surface；
Wherein, the length direction of overhead gage and lower baffle plate and the corner dimension of nutrient solution flow direction are 20~70 degree, and
Angle is in opposite direction；
Also include：Bioreactor culture liquid entrance 1, bioreactor culture liquid outlet 2, host film 5, gusset 6, drawstring 7,
Separated between the runner by gusset 6, the gusset 6 is illumination in the board-like bioreactor global formation of software
The host film 5 of face and dull surface is directly bonded or welded the bond line or soldered formed, plays demarcation strip.
Described bioreactor culture liquid entrance 1 and bioreactor culture liquid outlet 2 are diagonally arranged at the board-like light of software respectively
The one end of bioreactor upper runner nutrient solution entrance 8 and runner nutrient solution export 9 one end, bioreactor culture liquid entrance 1 and runner
Nutrient solution entrance 8 forms bioreactor culture liquid entrance cavity 10, and bioreactor culture liquid outlet 2 forms anti-with runner nutrient solution outlet 9
Answer device nutrient solution outlet plenum 11；
A drawstring 7, the drawing are provided with the bioreactor culture liquid entrance cavity 10 and/or bioreactor culture liquid outlet plenum 11
Band 7 is a software film, and its lower edges is directly bonded or is welded on bioreactor inwall.
The material thickness at the position of gusset 6 is more than or equal to other portions on the board-like bioreactor host film 5 of software
The material thickness of position.The material thickness at the position of gusset 6 is preferably the 1.1-3 of other part material thickness on the host film 5
Times.
When full of nutrient solution, edge extends described bioreactor culture liquid entrance cavity 10 perpendicular to runner nutrient solution entrance 8
The sectional area in line direction is gradually reduced along nutrient solution flow direction, and the sectional area ratio at maximum and minimum is 2:1-5:1.
Described bioreactor culture liquid outlet plenum 11 extends when full of nutrient solution along perpendicular to runner nutrient solution outlet 9
The sectional area in line direction gradually increases along nutrient solution flow direction, and the sectional area ratio at maximum and minimum is 2:1-5:1.
The sectional area of the bioreactor culture liquid entrance cavity 10 is gradually reduced along nutrient solution flow direction, is avoided in China
There is no the liquid linear velocity at the both ends of entrance cavity and pressure gap in the scheme of reducing excessive in patent CN201010193837.4
(liquid linear velocity and pressure close to the end of bioreactor culture liquid entrance 1 are very high, away from bioreactor culture liquid entrance 1
The liquid linear velocity and pressure of end are very low), the flow velocity in each runner is inconsistent, the portion of runner efficiency of light energy utilization is relatively low
Problem so that the linear velocity of nutrient solution and pressure evenly, also cause this in Photoreactor nutrient solution entrance cavity 10 of the invention
Culture flow velocity in each runner in parallel of the Photoreactor of invention is more balanced, the line of nutrient solution in nutrient solution entrance cavity 10
So as to reduce, (nutrient solution is from Photoreactor nutrient solution entrance cavity 10 closer to the culture flow velocity in each runner in parallel for speed
During into each runner in parallel) disturbance in flow field and flow resistance at runner nutrient solution entrance 8.The bioreactor culture liquid goes out
The effect that the sectional area in oral cavity 11 gradually increases along nutrient solution flow direction is similar therewith.
The height of described drawstring 7 is the bioreactor culture liquid entrance cavity 10 or bioreactor culture liquid outlet plenum 11 where it
Corresponding site width 20%-80%, wherein, described " width of corresponding site " refers to that bioreactor is not filled with
The bioreactor culture liquid entrance cavity 10 or the width of the corresponding site of bioreactor culture liquid outlet plenum 11 integrally deployed during nutrient solution,
That is, the height of the drawstring of bioreactor culture liquid entrance cavity 10 is gradually reduced along nutrient solution flow direction, the outlet of bioreactor culture liquid
The height of the drawstring of chamber 11 gradually increases along nutrient solution flow direction.
The length of described drawstring 7 is the 30%-80% of bioreactor width, wherein, described " optical-biological reaction
Device width " refers to that bioreactor is not filled with the width integrally deployed during nutrient solution.
Described drawstring 7 can be complete software film or the software film for being provided with one or more holes.Draw
The section in the hole taken can be circle, rectangle, trapezoidal, triangle or other shapes, the diameter in the hole or the lower edges in hole
Distance be hole position drawstring height 10%-70%.
Described overhead gage 3 and the section of lower baffle plate 4 can be for rectangle, trapezoidal, triangle, semicircle or other shapes (such as
Shown in Fig. 5)；Wherein, the thickness of the baffle plate of rectangular shape is 1-5mm；Half eminence of trapezoidal, triangle or semicircular baffle plate
Thickness is 1-5mm.
In the board-like bioreactor of described software along flow channel length direction two adjacent screens of the same face spacing
For 10-200mm.
The projected length of overhead gage 3, lower baffle plate 4 along width of flow path direction is in the board-like bioreactor of described software
The 10%-100% of the board-like bioreactor width of flow path of the software, wherein, described " bioreactor width of flow path "
Refer to that bioreactor is not filled with the width of the runner integrally deployed during nutrient solution.
The height (i.e. the height in baffle plate itself section) of overhead gage 3 and lower baffle plate 4 of the present invention is 1-15mm.
The material of main part (material of host film 5) of the board-like bioreactor of described software is software light transmissive material,
Described software light transmissive material can be one kind in polyethylene, polyvinyl chloride, polypropylene, polyester, thermoplastic elastomer (TPE) etc..
The material of main part of the board-like bioreactor of described software can further select printing opacity and ventilative material, preferably
Ground, material of main part are not through the software light transmission film material of carbon dioxide for permeable oxygen.
The thickness of described software light transmissive material is 0.1-2mm.
Can be according to the manufacture that film is thickeied at the processing characteristics selection gusset 6 of material.For example, can be thin by strip software
Film is bonded on the internal face at the position of software bioreactor gusset 6, can also directly be processed with mould gusset position with
The different film of other part thickness.
Described tape material can be software light transmissive material or light-proof material, and described light transmissive material can be poly- second
One kind in alkene, polyvinyl chloride, polypropylene, polyester and thermoplastic elastomer (TPE) etc.；Described light-proof material can be plastics, rubber
One kind in glue and resin etc.；Tape material and material of main part are identical or different, it is preferable that the material and light of the drawstring
The material of main part of bioreactor is identical.
To reach the technique effect of the present invention, when bioreactor is not filled with nutrient solution and integrally deployed, in runner
On the section of nutrient solution flow direction, the view field of the overhead gage and lower baffle plate can be with overlapping, i.e., described upper gear
Plate and lower baffle plate can be mutually twisted, the spacing depending on adjacent screen；When bioreactor is full of nutrient solution, in runner
Interior the view field of the overhead gage and lower baffle plate is not overlapping on the section of nutrient solution flow direction, i.e., described upper gear
Plate and lower baffle plate will not be mutually twisted.
Described baffle plate can be solid construction or hollow-core construction.
The material of described baffle plate can be software light transmissive material or light-proof material, and described light transmissive material can be poly-
One kind in ethene, polyvinyl chloride, polypropylene, polyester and thermoplastic elastomer (TPE) etc.；Described light-proof material can be plastics,
One kind in rubber and resin etc.；Barrier material and material of main part are identical or different, it is preferable that the material of the baffle plate with
The material of main part of bioreactor is identical.
The board-like bioreactor baffle plate of software of the present invention can use bonding, hot pressing, height with software light transmission film material
Frequency welding, ultrasonic bonding the methods of be bonded together, can also by rolling, squeeze piece, extrusion it is compound, plastic uptake is compound the methods of
Make baffle plate one-shot forming together with software light transmission film material.
The board-like bioreactor of software of the present invention is the direct side such as bonding, hot pressing, high-frequency welding, ultrasonic bonding
Method is by reactor body material software light transmission film material (such as coiled material or membrane material) at gusset 6, at drawstring and surrounding edge
Place is bonded together, and runner is softened so as to which Formation cross-section is circular or ellipse runner after being filled with nutrient solution.
When nutrient solution flows through the board-like bioreactor of the software, spiral motion can be formed in runner, and
And whirlpool can be produced between baffle plate, and promote microalgae cell to be upwardly formed certain reciprocating motion in optical path side, it is micro- so as to realize
Frustule shuttles the light area of the board-like bioreactor of software and dark space, as shown in Figure 4.
Between the gusset and gusset of the board-like bioreactor of software of the present invention, between outermost gusset and reactor wall
Form runner parallel with one another, bioreactor length, bioreactor width, bioreactor flow channel length, light
The quantity of bioreactor width of flow path and bioreactor runner can make corresponding adjustment according to being actually needed, and be formed different
The board-like bioreactor of software of profile.Wherein the quantity of runner is 2-50, preferably 5-30, most preferably 10-20.
When microalgae cell is cultivated in the board-like bioreactor of software of the present invention, nutrient solution is in bioreactor stream
Flow velocity in road is 0.1-1m/s, and the flow velocity refers to superficial linear vilocity.
, can be by multiple board-like bioreactors of software of the invention according to the requirement of yield during extensive microalgae production
In parallel or series, then supporting other devices.For example, as shown in Figure 6 and Figure 7, during actual production culture microalgae cell,
In whole culture systems in addition to the board-like bioreactor 18 of above-mentioned software of the invention, nutrient solution storage dress is also equipped with
Put 16, nutrient solution conveying device 17, gas-liquid exchange device 19 is used to supplying and carrying out oxygen parsing, CO2Supplementary device 20 and company
Other auxiliary equipments such as connection device, material distribution device, temperature monitor and control device.
The board-like bioreactor of software of the present invention is suitable for micro-algae large-scale culture under various climatic environments, can be with
It is built in outdoor outdoor or greenhouse, directly can lays or be covered in the land such as top of building, Desertification Soil, sandy beach
Surface, the water surfaces such as river, lake, ocean can be swum in, make full use of natural light.Various microalgaes can be trained
Support, including the one or more in spirulina, chlorella, salt algae, grid algae, diatom, chrysophyceae and plan microballoon algae.
Compared with CN201010193837.4 board-like bioreactor, the board-like photo-biological of software provided by the invention is anti-
The advantage of device is answered to be：
1st, the overall structure of bioreactor is optimized, there is provided the reaction changed along nutrient solution flow direction sectional area
Device nutrient solution entrance cavity 10 and bioreactor culture liquid outlet plenum 11, the stream of algae solution in different runners is found using CFD software simulation
Fast basis equalization (as shown in figure 3, the different colours depth represents different flow velocitys in flow field simulation figure), algae equally in each runner
Light area/dark space conversion frequency of cell is basically identical (as shown in figure 4, movement locus of microalgae cell shown in Fig. 4), so that
The efficiency of light energy utilization of each runner of bioreactor is basically identical, improves the overall light-use effect of bioreactor
Rate.
2nd, bioreactor culture liquid entrance cavity 10 and bioreactor culture liquid outlet plenum 11 are provided with drawstring so that the board-like light of software
The pressure-resistant and impact resistance of bioreactor greatly improves, and improves the service life of the board-like bioreactor of software.
3rd, the software film thickness at the board-like bioreactor gusset 6 of software is more than or equal to other position films, improves
Intensity at gusset, bioreactor is reduced because the reasons such as aging, stress cause the danger of rupture, improves software plate
The service life of formula bioreactor.
4th, the board-like bioreactor of software of the invention is as a result of software light transmission film material so that photo-biological is anti-
Device cost is answered to be greatly lowered, batch cost is about per 50 yuan of square meter, the board-like photo-biological made well below in general of glass
Reactor.
5th, can be by the board-like bioreactor serial or parallel connection of multiple monomer softwares, thus substantially reduce operation and maintenance
Cost；It can directly tile without hardened ground, can quickly connect into culture systems, rapid construction, as shown in Figure 8；
Especially, can be direct by the system of the board-like bioreactor serial or parallel connection of multiple monomer softwares in Gobi Desert Terrain area
It is covered on sand ground, on the one hand by the gap Accumulated Rainwater between monomer, on the one hand reduces evaporation capacity, is Gobi Desert Terrain area
Water-retaining effect.
Brief description of the drawings
Fig. 1 is the top view of the board-like bioreactor of software of the present invention.
Fig. 2 is the drawstring and schematic view of the mounting position of the present invention.
Fig. 3 is the flow field simulation figure in the board-like bioreactor of software of the present invention.
Fig. 4 is the change in location of frustule in the board-like bioreactor of software of the present invention.
Fig. 5 is the front view of single several different cross section shapes of baffle plate on baffled film of the present invention.
Fig. 6 is the microalgae culture system schematic diagram using the board-like bioreactor series connection of multiple softwares of the present invention.
Fig. 7 is using the board-like bioreactor of multiple softwares of present invention microalgae culture system schematic diagram in parallel.
Fig. 8 is the microalgae culture system using the board-like bioreactor series connection of multiple softwares of the present invention.
Accompanying drawing identifies
1st, bioreactor culture liquid entrance 2, bioreactor culture liquid outlet 3, overhead gage
4th, lower baffle plate 5, host film 6, gusset
7th, drawstring 8, runner nutrient solution entrance
9th, runner nutrient solution outlet 10, bioreactor culture liquid entrance cavity
11st, bioreactor culture liquid outlet plenum 12, rectangle baffle plate 13, triangular baffle
14th, trapezoidal baffle plate 15, Semicircular baffle 16, nutrient solution storage device
17th, the board-like bioreactor of nutrient solution conveying device 18, software
19th, gas exchange device 20, CO2Supplementary device
Embodiment
Below in conjunction with the accompanying drawings and embodiment the invention will be further described.
Embodiment 1
The culture of spirulina is carried out under outdoor natural light photograph.The culture systems of structure include a board-like photo-biological of software
Reactor 18, nutrient solution storage device 16, nutrient solution conveying device 17 (commercially available membrane pump), gas-liquid exchange device 19 and CO2Mend
Fill and put 20, connected mode is as shown in Figure 6.
The structure of the board-like bioreactor 18 of software is as shown in Figure 1.The board-like bioreactor of software is laid in ground
On, main material is software printing opacity polyethylene film.The board-like bioreactor of software long 200cm, wide 96cm, are divided into 16 phases
Mutually runner in parallel；The thickness of host film 5 is 0.5mm, and film thickness 0.75mm at gusset 6, the length of gusset 6 is 140cm；Instead
It is 2 to answer the sectional area ratio at device nutrient solution entrance cavity 10 and the maximum of bioreactor culture liquid outlet plenum 11 and minimum:1；Upper gear
Plate 3 and lower baffle plate 4 are rectangle baffle plate, be highly 10mm, width 2.5mm, length 6mm, with flow of fluid angle be 30 °,
12 pieces of overhead gages of installation and 12 pieces of lower baffle plates in each runner；Drawstring 7 is provided with 4 circular apertures, and the length of drawstring 7 is
48cm, the height of drawstring 7 are the corresponding portion of the bioreactor culture liquid entrance cavity 10 or bioreactor culture liquid outlet plenum 11 where it
The 50% of the width of position.
Algae kind comes from Chinese Academy Of Sciences Process Engineering Research Institute, and kind is blunt top spirulina, and culture medium is cultivated for Zarrouk
Base, the initial concentration of sodium acid carbonate is 0.1mol/L in nutrient solution.
Above-mentioned bioreactor is cleaned with the water after 0.2 μm of micro-filtrate membrane filtration before culture.Cultivated according to Zarrouk
Based formulas prepares 50L culture mediums, 0.2 μ micro-filtrate membrane filtrations of culture medium.Kind of a liquid, inoculum density 0.1g/L are prepared according to a conventional method.
Medium pH value is set in 9.0, and 25~35 DEG C of environment temperature, using outdoor natural lighting, it is 26 ± 1 DEG C to control cultivation temperature.
Flow velocity of the nutrient solution in bioreactor runner is 20cm/s, the apparent pressure at bioreactor nutrient solution entrance 1
For 6kPa.During culture, air is passed through in the gas-liquid exchange device 19 into culture systems, carries out the releasing of dissolved oxygen so that
Bioreactor culture liquid exit nutrient solution saturation dissolved oxygen is less than 300%；Fed back by pH value and mend carbon mode to CO2Supplement dress
Put and CO is passed through in 202.Period sampling measuring algae cell density, start semicontinuous adopt when algae cell density reaches 3g (dry weight)/L
Receive, collecting method is drawn 20% of nutrient solution in culture systems, and the filtered fluid after filtering is returned into former training
In the system of supporting.Wash, dry after the frustule harvest being filtrated to get.
The concentration of the other nutritive salt of periodic detection and supplemented in time during culture, and supplement a small amount of water to make up the evaporation of water
Loss.Incubation continues 30 days, and photo-bioreactor system remains to normal operation, and the area yield of spirulina is about 40g/
m2.d。
Comparative example 1
The culture of spirulina is carried out under the conditions of outdoor natural light.Other conditions are with embodiment 1, except that therein
Bioreactor is that bioreactor culture liquid entrance cavity as shown in CN201010193837.4 accompanying drawings 3 and bioreactor culture liquid go out
Oral cavity sectional area is unchanged, is not provided with drawstring, the bioreactor that gusset position is non-reinforced.
Incubation continues 5 days, and bioreactor ruptures at the 5th day, and the area yield of spirulina is 33g/m2.d。
Knowable to comparative example 1 and comparative example 1, the board-like optical-biological reaction of software of the inside enhancing of the embodiment of the present invention 1
Service life of the device service life higher than the bioreactor of comparative example 1；And under same condition of culture, the embodiment of the present invention
In the 1 bioreactor culture liquid entrance cavity for being provided with sectional area change and the bioreactor of bioreactor culture liquid outlet plenum
Area yield is higher than the area yield in the bioreactor of comparative example 1.The bioreactor of the present invention uses the longevity
The life and efficiency of light energy utilization of frustule is obviously improved during for microdisk electrode.
Embodiment 2
The culture of spirulina is carried out under outdoor natural light photograph, other conditions are with embodiment 1, except that photo-biological is anti-
Answer 2 times that the part thickness of device gusset 6 is other part thickness.
Incubation continues 60 days, and bioreactor remains to normal operation, and the area yield of spirulina is about 39g/
m2.d, bioreactor service life is far above the bioreactor service life in comparative example 1；Area yield is higher than
The area yield in bioreactor in comparative example 1.The present invention bioreactor service life and for microalgae
Efficiency of light energy utilization during culture significantly improves.
Embodiment 3
The culture of spirulina is carried out under outdoor natural light photograph, other conditions are with embodiment 1, except that photo-biological is anti-
The sectional area ratio in device at bioreactor culture liquid entrance cavity 10 and the maximum of bioreactor culture liquid outlet plenum 11 and minimum is answered to be
4:1。
Incubation continues 30 days, and bioreactor remains to normal operation, and the area yield of spirulina is about 37g/
m2.d, bioreactor service life is far above the bioreactor service life in comparative example 1；Area yield is higher than
The area yield in bioreactor in comparative example 1.The present invention bioreactor service life and for microalgae
Efficiency of light energy utilization during culture significantly improves.
Embodiment 4
The culture of spirulina is carried out under outdoor natural light photograph, other conditions are with embodiment 1, except that photo-biological is anti-
The width for answering drawstring 7 in device is the 70% of bioreactor width, and the height of drawstring 7 is that the bioreactor culture liquid where it enters
The 60% of the width of the corresponding site of oral cavity 10 or bioreactor culture liquid outlet plenum 11.
Incubation continues 30 days, and bioreactor remains to normal operation, and the area yield of spirulina is about 38g/
m2.d, bioreactor service life is far above the bioreactor service life in comparative example 1；Area yield is higher than
The area yield in bioreactor in comparative example 1.The present invention bioreactor service life and for microalgae
Efficiency of light energy utilization during culture significantly improves.
Embodiment 5
The culture of spirulina is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that nutrient solution exists
Flow velocity in bioreactor is 50cm/s.
Incubation continues 30 days, and bioreactor remains to normal operation, and the area yield of spirulina is about 45g/
m2.d.Under higher flow rate and pressure, bioreactor remains to long-time normal operation, and area yield is apparently higher than comparative example
The area yield of bioreactor in 1.The service life of the bioreactor of the present invention and during for microdisk electrode
The efficiency of light energy utilization significantly improves.
Embodiment 6
The culture of spirulina is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that software is board-like
Bioreactor 18 long 200cm, wide 100cm, are divided into 10 runners parallel with one another.Incubation continues 30 days, spirulina
Area yield be about 35g/m2.d。
The board-like bioreactor service life of software and area yield of the inside enhancing of the embodiment of the present invention 6 are above
The service life and area yield of the bioreactor of comparative example 2, i.e., the service life of bioreactor of the invention and
The efficiency of light energy utilization of frustule is obviously improved during for microdisk electrode.
Comparative example 2
The culture of spirulina is carried out under the conditions of outdoor natural light.Other conditions are with embodiment 6, except that therein
Bioreactor is that bioreactor culture liquid entrance cavity as shown in CN201010193837.4 accompanying drawings 3 and bioreactor culture liquid go out
Oral cavity sectional area is unchanged, is not provided with drawstring, the bioreactor that gusset position is non-reinforced.
Incubation continues 8 days, and bioreactor ruptures at the 8th day, and the area yield of spirulina is 30g/m2.d。
Embodiment 7
The culture of chlorella is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that using BG11
Culture medium, utilize CO2As carbon source.Incubation continues 30 days, and the area yield of chlorella is about 25g/m2.d。
Obviously, the service life in the bioreactor of the embodiment of the present invention 7 and area yield are apparently higher than comparative example 3
Bioreactor service life and area yield, show the present invention bioreactor service life and luminous energy profit
Significantly improved with rate.
Comparative example 3
The culture of chlorella is carried out under the conditions of outdoor natural light.Other conditions such as embodiment 7, except that therein
Bioreactor is that bioreactor culture liquid entrance cavity as shown in CN201010193837.4 accompanying drawings 3 and bioreactor culture liquid go out
Oral cavity sectional area is unchanged, is not provided with drawstring, the bioreactor that gusset position is non-reinforced.Incubation continues 5 days, bead
The area yield of algae is about 20g/m2.d。
Embodiment 8
The culture of grid algae is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that being trained using BG11
Base is supported, utilizes CO2As carbon source.Incubation continues 30 days, and the area yield of grid algae is about 45g/m2.d。
Obviously, the service life in the bioreactor of the embodiment of the present invention 8 and area yield are apparently higher than comparative example 4
Bioreactor service life and area yield, show the present invention bioreactor service life and luminous energy profit
Significantly improved with rate.
Comparative example 4
The culture of grid algae is carried out under the conditions of outdoor natural light.Other conditions such as embodiment 8, except that light therein
Bioreactor is the bioreactor culture liquid entrance cavity and the outlet of bioreactor culture liquid as shown in CN201010193837.4 accompanying drawings 3
Chamber sectional area is unchanged, is not provided with drawstring, the bioreactor that gusset position is non-reinforced.Incubation continues 5 days, grid algae
Area yield is about 37g/m2.d。
Embodiment 9
Under outdoor natural light photograph intend the culture of microballoon algae.Other conditions are with embodiment 1, except that using people
Work seawater+1/2F culture mediums, utilize CO2As carbon source.Incubation continues 30 days, and the area yield for intending microballoon algae is about 22g/
m2.d。
Obviously, the service life in the bioreactor of the embodiment of the present invention 9 and area yield are apparently higher than comparative example 5
Bioreactor service life and area yield, show the present invention bioreactor service life and luminous energy profit
Significantly improved with rate.
Comparative example 5
Carry out intending the culture of microballoon algae under the conditions of outdoor natural light.Other conditions such as embodiment 9, except that wherein
Bioreactor be bioreactor culture liquid entrance cavity and bioreactor culture liquid as shown in CN201010193837.4 accompanying drawings 3
Outlet plenum sectional area is unchanged, is not provided with drawstring, the bioreactor that gusset position is non-reinforced.Incubation continues 5 days, intends
The area yield of microballoon algae is about 18g/m2.d。
Embodiment 10
The culture of spirulina is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that system includes
The board-like bioreactor 18 of above-mentioned software of 50 series connection.Incubation continues 30 days, and the area yield of spirulina is about
38g/m2.d。
Comparative example 10 and comparative example 6 are understood, board-like in the software of the present invention under more extensive microalgae culture system
Bioreactor still can be with normal operation, and has higher area yield, and the bioreactor large area of comparative example 6
Rupture, show that the bioreactor service life of the present invention significantly improves, can be used for the microdisk electrode of more large scale system.
Comparative example 6
Other conditions are with embodiment 10, except that bioreactor therein is as CN201010193837.4 is attached
Bioreactor culture liquid entrance cavity and bioreactor culture liquid outlet plenum sectional area shown in Fig. 3 are unchanged, are not provided with drawstring, gusset portion
The non-reinforced bioreactor in position.After water-filling, bioreactor many places rupture, and can not carry out microdisk electrode.
Embodiment 11
The culture of spirulina is carried out under outdoor natural light photograph.Other conditions are with embodiment 1, except that system includes
30 board-like bioreactors 18 of above-mentioned software in parallel.Incubation continues 30 days, and the area yield of spirulina is about
38g/m2.d。
Comparative example 11 and comparative example 7 understand there is longer make in the board-like bioreactor of software of the present invention
With life-span and higher area yield, i.e. bioreactor service life and the efficiency of light energy utilization of the invention significantly improves.
Comparative example 7
Other conditions are with embodiment 11, except that bioreactor therein is as CN201010193837.4 is attached
Bioreactor culture liquid entrance cavity and bioreactor culture liquid outlet plenum sectional area shown in Fig. 3 are unchanged, are not provided with drawstring, gusset portion
The non-reinforced bioreactor in position.Incubation continues 5 days, and part bioreactor starts to rupture, the area of spirulina
Yield is about 38g/m2.d。
Claims (12)
1. a kind of board-like bioreactor of software, including a runner or at least two runners parallel with one another, described every
One runner includes：
- at least 2 pieces of overhead gages (3), are arranged on the internal face of plane of illumination；
- at least 2 pieces of lower baffle plates (4), are arranged on the internal face of dull surface；
Wherein, the length direction of overhead gage (3) and lower baffle plate (4) and the corner dimension of nutrient solution flow direction are 20~70 degree,
And angle is in opposite direction；
The board-like bioreactor of software also includes bioreactor culture liquid entrance (1), bioreactor culture liquid outlet (2), master
Body thin film (5), gusset (6) and drawstring (7), it is characterised in that separated between the runner by gusset (6), the gusset (6) is
The host film of plane of illumination and dull surface (5) is directly bonded or welded formation in the board-like bioreactor global formation of software
Bond line or soldered；
Described bioreactor culture liquid entrance (1) and bioreactor culture liquid outlet (2) are diagonally arranged at the board-like light of software respectively
Bioreactor upper runner nutrient solution entrance (8) one end and runner nutrient solution outlet (9) one end, bioreactor culture liquid entrance (1)
Bioreactor culture liquid entrance cavity (10), bioreactor culture liquid outlet (2) and runner nutrient solution are formed with runner nutrient solution entrance (8)
Export (9) and form bioreactor culture liquid outlet plenum (11)；
Wherein, described bioreactor culture liquid entrance cavity (10) is when full of nutrient solution, along perpendicular to runner nutrient solution entrance (8)
The sectional area in extended line direction is gradually reduced along nutrient solution flow direction, and the sectional area ratio at maximum and minimum is 2:1-5:
1；
Described bioreactor culture liquid outlet plenum (11) extends when full of nutrient solution along perpendicular to runner nutrient solution outlet (9)
The sectional area in line direction gradually increases along nutrient solution flow direction, and the sectional area ratio at maximum and minimum is 2:1-5:1；
A drawstring (7) is provided with the bioreactor culture liquid entrance cavity (10) and/or bioreactor culture liquid outlet plenum (11), it is described
Drawstring (7) is a software film, and its lower edges is directly bonded or is welded on bioreactor inwall.
2. the board-like bioreactor of software according to claim 1, it is characterised in that the board-like photo-biological of software is anti-
The material thickness at gusset (6) position on device host film (5) is answered to be more than or equal to the material thickness at other positions.
3. the board-like bioreactor of software according to claim 2, it is characterised in that muscle on the host film (5)
The material thickness at plate (6) position is 1.1-3 times of other part material thickness.
4. the board-like bioreactor of software according to claim 1, it is characterised in that the height of the drawstring (7) is
Bioreactor culture liquid entrance cavity (10) or bioreactor culture liquid outlet plenum (11) where it are not filled with the phase deployed during nutrient solution
Answer the 20%-80% of position width.
5. the board-like bioreactor of software according to claim 1, it is characterised in that the length of described drawstring (7)
The 30%-80% of the width deployed when not being filled with nutrient solution for board-like bioreactor.
6. the board-like bioreactor of software according to claim 1, it is characterised in that described drawstring (7) is complete
Software film, or be provided with the software film in one or more holes.
7. the board-like bioreactor of software according to claim 6, it is characterised in that the diameter in the hole or hole it is upper
The distance of lower edge is the 10%-70% of hole position drawstring height.
8. the board-like bioreactor of software according to claim 1, it is characterised in that the material of the host film (5)
Material is software light transmissive material；The material of the overhead gage (3), lower baffle plate (4) or drawstring (7) be software light transmissive material or software not
Light transmissive material.
9. the board-like bioreactor of software according to claim 8, it is characterised in that described software light transmissive material is
One kind in polyethylene, polyvinyl chloride, polypropylene, polyester or thermoplastic elastomer (TPE)；Described software light-proof material be plastics,
One kind in rubber or resin.
10. the board-like bioreactor of software according to claim 1, it is characterised in that the board-like photoproduction of described software
The spacing of two adjacent screens of the same face is 10-200mm along flow channel length direction in thing reactor.
11. the board-like bioreactor of software according to claim 1, it is characterised in that the board-like photoproduction of described software
The projected length of overhead gage (3) and lower baffle plate (4) along width of flow path direction is the board-like optical-biological reaction of the software in thing reactor
Device is not filled with the 10-100% of the width of flow path deployed during nutrient solution.
12. the board-like bioreactor of software according to claim 1, it is characterised in that described overhead gage (3) or under
The depth of section of baffle plate (4) is 1-15mm.
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| CN201410270445.1A CN105219616B (en) | 2014-06-17 | 2014-06-17 | A kind of board-like bioreactor of software |
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| CN201410270445.1A CN105219616B (en) | 2014-06-17 | 2014-06-17 | A kind of board-like bioreactor of software |
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| CN101899385B (en) * | 2009-05-27 | 2016-04-27 | 中国科学院过程工程研究所 | Improve the closed photo bioreactor of the efficiency of light energy utilization that microalgae mass is cultivated |
| CN102260629B (en) * | 2010-05-28 | 2014-07-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
| CN102373149B (en) * | 2010-08-24 | 2014-12-24 | 新奥科技发展有限公司 | Photobioreactor, its preparation method, photo-biological culture system and method |
| CN103361257A (en) * | 2012-04-05 | 2013-10-23 | 华东理工大学 | Light direction hybrid reinforcement-based novel internal component and photobioreactor |
| CN203923168U (en) * | 2014-06-17 | 2014-11-05 | 中国科学院过程工程研究所 | The board-like bioreactor of a kind of software |
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