CN101613659A - Microalgae cultivation accelerator - Google Patents

Microalgae cultivation accelerator Download PDF

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Publication number
CN101613659A
CN101613659A CN200910101170A CN200910101170A CN101613659A CN 101613659 A CN101613659 A CN 101613659A CN 200910101170 A CN200910101170 A CN 200910101170A CN 200910101170 A CN200910101170 A CN 200910101170A CN 101613659 A CN101613659 A CN 101613659A
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Prior art keywords
plate
diffuser
flowing water
side plate
grid plate
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CN200910101170A
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Inventor
吴洪喜
蔡景波
谢起浪
黄振华
吕立蒴
李磊
柯爱英
曾国权
陈琛
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Zhejiang Mariculture Research Institute
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Zhejiang Mariculture Research Institute
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Abstract

The present invention relates to a kind of auxiliary implement that is applicable to that microalgae is cultivated, microalgae cultivation accelerator in particular, can promote micro algae growth, thereby little algae output in the raising unit surface, this microalgae cultivation accelerator comprises at least one group of flowing water well and air feeder, retaining plate before and after described flowing water well comprises, left side plate and grid plate, left side plate and grid plate are connected and fixed with described front and back retaining plate respectively, the base of front and back retaining plate and grid plate maintains an equal level, the top margin of left side plate and front and back retaining plate all is higher than the top margin of grid plate, the base of retaining plate and grid plate before and after the base of left side plate is higher than, left side plate form current rising passway and current decline passway with grid plate respectively; The diffuser that described air feeder comprises air feeder and has sealed end, diffuser are positioned at described current rising passway, and the inlet end of diffuser passes rear fixed plate or front shoe and is connected to described air feeder.

Description

Microalgae cultivation accelerator
Technical field
The present invention relates to a kind of auxiliary implement that is applicable to that microalgae is cultivated, in particular microalgae cultivation accelerator.
Background technology
Microalgae is called for short little algae, is the tiny organism that just can distinguish its form at microscopically, lives in the sea water and fresh water with normally suspending, is minimum grade, a most ancient class plant, has found kind more than 20,000 at present.Little algae can pass through the photosynthesis stabilizing carbon dioxide; luminous energy is converted into organism; have that photosynthetic efficiency height, environmental compatibility are strong, breeding is fast, growth cycle lack, is not occupied cultivated land, the biological yield height, can change on a large scale, automatization control cultivation characteristics such as (productions).Little algae contains nutritive ingredients such as protein, fat, sugar and amino acid, be the good bait of hydrocoles, especially essential open-mouthed bait and the main bait of most of fishes and shrimps shellfish young, the little algae of some kind also contains abundant highly unsaturated fatty acids or protein, can be used as human healthcare products.Other has little algae of some kinds can also be used for producing fat, hydrogen or hydrocarbons, also can use it for extracting gasoline, diesel oil, Aviation Fuel as energy fuel.
Certain slightly algae not only have good nutrition, and palatability and mobility are good, also can improve the water quality of growing seedlings to a certain extent, thereby play irreplaceable effect in aquatic product sprout.But the production of present little algae is still very backward, generally adopt little algae cultivation pool or little algae culture vessel to produce, output is not high, be difficult to satisfy the demand that aquatic product sprout is cultivated, influenced the economic benefit of aquatic fry growing manufacturing enterprise, and the breed scale of current continuous expansion has further been aggravated the imbalance between supply and demand of little algae, the underproduce development that restricts culture fishery to a great extent of little algae, and therefore current urgent need improves the output of little algae to satisfy the demand in market.
Summary of the invention
Cultivate unhappy, the underproduce defective of speed at present little algae, the invention provides microalgae cultivation accelerator, this new device provides and can promote micro algae growth, thereby improves little algae output in the unit surface.
Microalgae cultivation accelerator provided by the present invention, comprise at least one group of flowing water well and air feeder, described flowing water well comprises left and right sides retaining plate, front and back side plate and grid plate, front and back side plate and grid plate are connected and fixed with described left and right sides retaining plate respectively, the base of left and right sides retaining plate and grid plate maintains an equal level, the top margin of front and back side plate and left and right sides retaining plate all is higher than the top margin of grid plate, the base of retaining plate and grid plate before and after the base of front and back side plate is higher than, the front and back side plate forms current rising passway and current decline passway with grid plate respectively; The diffuser that described air feeder comprises air feeder and has sealed end, diffuser are positioned at described current rising passway, and the inlet end of diffuser passes right retaining plate or left retaining plate and is connected to described air feeder.
Because the factor of the influence breeding of little algae and growth is except itself biological characteristics, main just relevant with the luminous energy supply, so reaching little algae produces fast and aim of high yield, except little algae needs the sufficient sunlight with cultivating, the position of microalgae cell in culture vessel and be subjected to the light path degree also very big to the influence of its breeding and the speed of growth.If the microalgae cell in therefore can thorough mixing culture vessel levels, the microalgae cell that guarantees levels all can absorption sunlight as much as possible, will help improving the utilization ratio of little algae output and cultivation pool or culture vessel.Based on aforementioned principles, the grid plate that this microalgae cultivation accelerator provided is two portions with the space segmentation of front and back side plate and the formation of left and right sides retaining plate, and because the base of left and right sides retaining plate and grid plate maintains an equal level, the top margin of front and back side plate and left and right sides retaining plate all is higher than the top margin of grid plate, makes flowing water well inside form current rising passway and current decline passway respectively.In the current rising passway, diffuser is installed, one end is a sealed end, the other end is inlet end and connects air feeder, so the gas that air feeder provides rises in the current rising passway through diffuser diffusion back, be subjected to the pulling of ascending gas in the current rising passway, water level raises in the flowing water well, in the current direction well outside the flowing water well, current are crossed the grid plate overhead stream to adjacent current decline passway, and last water outflow flowing water well comes back to little algae cultivation pool or culture vessel recycles.Because little algae is to live in the water with suspended state, can be along with current rise and fall in the flowing water well, so exist the little algae in the subjacent waters just can obtain the chance that the luminous energy identical with the little algae in the superjacent waters absorbs originally, and the little algae in the levels waters is mixed, promote the growth and breeding of little algae, can improve little algae output more than 30%, or the little algae that produces identical output can shorten incubation time more than 1/3rd, thereby improve the utilization ratio of little algae cultivation pool or culture vessel.Owing to use this auxiliary implement not need cultivation pool or culture vessel are transformed, decline algae cultivation pool or culture vessel of any indoor and outdoor opening all can be placed the cultivation accelerator of respective number according to own vol, has the strong advantage of suitability, can be used as the good auxiliary implement that at present domestic little algae produces, have wide industrial application prospect.
The progressive scheme of technique scheme is to have five groups of continuously arranged flowing water wells in the microalgae cultivation accelerator, five groups of flowing water wells comprise integrated left and right sides retaining plate and six blocks of side plates that uniformly-spaced are fixed on successively on the retaining plate of the left and right sides, be equipped with the grid plate parallel between every adjacent biside plate with side plate, grid plate is fixed on the retaining plate of the left and right sides, biside plate that each piece grid plate is adjacent and left and right sides retaining plate constitute one group of flowing water well, be provided with diffuser in the current rising passway in each group flowing water well, the inlet end of each diffuser is connected to described air feeder through same pneumatic tube.
The flowing water well of improved five groups of integral types like this not only sound construction is processed with being easy to, and can make current and little algae continuously through the stroke of five flowing water wells, under the prerequisite that it is suitable that the flowing water well takes up room, satisfy the requirement of micro algae growth to greatest extent to required luminous energy.
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail.
Description of drawings
Fig. 1 is the structural representation that the embodiment of microalgae cultivation accelerator of the present invention is provided with single flowing water well.
Fig. 2 is the structural representation that five groups of Continuous Flow wells are set on the basis of Fig. 1 embodiment.
Fig. 3 is the principle of work synoptic diagram of the embodiment of microalgae cultivation accelerator of the present invention.
Embodiment
As Fig. 2 and in conjunction with shown in Figure 1, the embodiment of microalgae cultivation accelerator of the present invention, comprise five groups of continuously arranged flowing water wells 1 and air feeder, the left retaining plate a and the right retaining plate of five groups of flowing water wells 1 are respectively one-body molded manufacturing, six side plate b equally spaced are fixed on the retaining plate of the left and right sides front and back successively, be equipped with the grid plate c parallel between every adjacent front and back biside plate b, amount to five grid plate c with side plate b.Biside plate b and left and right sides retaining plate a that each piece grid plate c is adjacent constitute one group of flowing water well 1.Side plate b, grid plate c in five groups of flowing water wells 1 is all perpendicular to left and right sides retaining plate and be parallel to each other, side plate b and grid plate c are connected and fixed with described left and right sides retaining plate respectively, the left and right sides retaining plate of five groups of flowing water wells 1 and the base of grid plate c maintain an equal level, and promptly the base of left and right sides retaining plate and grid plate c can make the bottom of embodiment be smooth horizontal bottom so that fit with the flat bottom of little algae cultivation pool or culture vessel.The left and right sides retaining plate of present embodiment, six side plate b and five grid plate c are that the synthetic glass by 5 mm thick is made, has high light transmittance, and each plate place of being connected and fixed all adopts synthetic glass glue bonding, the bonding place is with the bonding reinforcing of synthetic glass set square, to strengthen resistance to tension and work-ing life.The size of left and right sides retaining plate can preferably widely be that 150 centimetres, height are 80 centimetres, between the retaining plate of the left and right sides at a distance of 80 centimetres, the size of side plate b can preferably widely be that 80 centimetres, height are 70 centimetres, the gravel size decision of grid plate c is wide to be that 80 centimetres, height are 70 centimetres, the top margin of side plate b and left and right sides retaining plate all is higher than 10 centimetres of the top margins of grid plate c, the base of side plate b is higher than 10 centimetres on the base of left and right sides retaining plate and grid plate c, and above-mentioned listed size can be considered the widest size of the scope of application.Distance all is 15 centimetres between the grid plate c of every group of flowing water well 1 and the side plate b, adjacent biside plate b respectively be positioned at its central grid plate c and form current rising passway and current decline passway; Five groups of shared air feeder (not shown) of flowing water well, in each group flowing water well water stream rising passway a diffuser 2 is set, diffuser 2 use internal diameters are 1 centimetre pipe, for improving the diffusing gas effect of diffuser, guarantee the proper flow of current in the current rising passway, the special improvement structure of diffuser 2 is before and after it that side is equipped with row's air dispelling hole (among the figure for showing), between each air dispelling hole of front-seat or back bat all at a distance of 1 centimetre of proper alignment.Five diffusers 2 are all perpendicular to left and right sides retaining plate and be parallel to each other, the base of five diffusers 2 and side plate b, five diffusers 2 that maintain an equal level promptly are higher than 10 centimetres on the base of left and right sides retaining plate and grid plate c equally, can make the pulling effect of ascending gas reach optimum regime so to greatest extent.Each diffuser 2 right-hand member is fixedly mounted on the right retaining plate hermetically, especially hermetically be installed on the diffuser seat 5 of right retaining plate of flowing water well 1, each diffuser 2 left end is an inlet end, described inlet end passes the left retaining plate a of flowing water well 1, for ease of connecting, the inlet end of five diffusers 2 is connected on the same pneumatic tube 3, pneumatic tube 3 is connected on the described air feeder, and can use hose connection diffuser 2 inlet ends of PVC and pneumatic tube 3 between two pipes, not to reveal to guarantee gas, air feeder can be selected Root's blower for use, air compressor, machines in normal service such as compact electromagnetic air pump, the air demand that sets need guarantee that the water surface that makes the current rising passway is " boiling " shape.
Using the foregoing description to carry out little algae, to quicken the step of cultivating as follows: the density by little algae cultivation pool or culture vessel area 3-6 square metre 1 is placed, connect airing system, disinfect (method of handling seawater with traditional algae culture) after adding the sterilized water of handling well in advance again or adding water, add substratum at last and connect the algae kind, make elevation of water surface exceed 1-2 centimetre, connect the air feeder power supply and get final product than the top margin of grid plate.As shown in Figure 3, pulling by ascending gas in being subjected in the current rising passway, in the current direction well outside the flowing water well, current flow to adjacent current decline passway from the grid plate top margin, under adjacent current rising passway water body pulling function, flow to adjacent current rising passway, thus repeatedly, last water and little algae flow into cultivation pool or culture vessel by the embodiment water outlet.As in a Da Chi, using apparatus of the present invention simultaneously, each device is placed in the same way, by circumferential direction to reach best acceleration culture effect.
Below be the application test of above-mentioned microalgae cultivation accelerator in little algae is cultivated:
1 materials and methods
1.1 algae kind
Inferior heart-shaped flat algaes etc., the equilateral chrysophyceae of ball derive from base, Zhejiang Mariculture Research Institute Yongxing.
1.2 plant and instrument
Microalgae cultivation accelerator; Cultivation pool: 2 mouthfuls of 5 * 6m 2The indoor cement pit of the transparent sunlight of top cover watt; Gas meter; Blood counting chamber; Microscope.
1.3 test method
At 1 mouthful of 5 * 6m 2Cement pit central authorities place 2 " series connection " microalgae cultivation accelerators, Xiang Chizhong adds seawater and reaches depth of water 45cm, then with the chlorinated lime seawater of sterilizing, in the inferior daily Sulfothiorine and chlorine residue, adds access algae kind behind the substratum.Test is provided with a parallel contrast pond, in the parallel contrast pond, selects traditional natural aquaculture model at present, test tank and to contrast conditions such as total aeration quantity in the pond, inoculum density, intensity of illumination all identical.
Measure 1.4 observe
Inoculation time is selected in point in fine mornings 8, and duration of test 16:00 in afternoon is the microalgae cell density in determination test pond and the contrast pond etc. simultaneously, and sampling method is respectively got 1 sample 4 jiaos of the pond and centre, and determination data is calculated mean value then.
1.5 data analysis and figure list processing
Figure G2009101011708D00061
Figure G2009101011708D00071
Can see by form, after the 3rd day, the content of little algae has had significant difference in two kinds of processing, little algae density of microalgae cultivation accelerator than control group height more than 1/3, at the 9th day, the little algae density of the test tank of using miniature algae culture accelerator reached 109 * 10 4/ mL has reached the concentration requirement of throwing something and feeding.Hence one can see that uses microalgae cultivation accelerator can improve little algae output more than 30%, or the little algae that produces identical output can shorten incubation time more than 1/3rd.

Claims (10)

1, a kind of microalgae cultivation accelerator, it is characterized in that: comprise at least one group of flowing water well (1) and air feeder, retaining plate (a) before and after described flowing water well (1) comprises, left side plate (b) and grid plate (c), left side plate (b) and grid plate (c) are connected and fixed with described front and back retaining plate (a) respectively, front and back retaining plates (a) maintain an equal level with the base of grid plate (c), the top margin of left side plate (b) and front and back retaining plate (a) all is higher than the top margin of grid plate (c), the base of retaining plate (a) and grid plate (c) before and after the base of left side plate (b) is higher than, left side plate (b) form current rising passway and current decline passway with grid plate (c) respectively; Described air feeder comprises air feeder and has the diffuser of sealed end (2), and diffuser (2) is positioned at described current rising passway, and the inlet end of diffuser (2) passes rear fixed plate or front shoe (a) and is connected to described air feeder.
2, microalgae cultivation accelerator according to claim 1, it is characterized in that: have five groups of continuously arranged flowing water wells (1), five groups of flowing water wells (1) comprise integrated front and back retaining plates (a) and six blocks of side plates (b) that uniformly-spaced are fixed on successively on the front and back retaining plates (a), be equipped with the grid plate parallel (c) between every adjacent biside plate (b) with side plate, grid plate (c) is fixed on the front and back retaining plates (a), biside plate (b) that each piece grid plate (c) is adjacent and front and back retaining plate (a) constitute one group of flowing water well (1), be provided with diffuser (2) in each group flowing water well (1), the inlet end of each diffuser (2) is connected to described air feeder through same pneumatic tube (3).
3, microalgae cultivation accelerator according to claim 1 and 2 is characterized in that: described side plate (b), grid plate (c) and diffuser (2) are all perpendicular to front and back retaining plate (a), and the base of diffuser (2) and side plate (b) maintains an equal level.
4, microalgae cultivation accelerator according to claim 1 and 2 is characterized in that: be equipped with row's air dispelling hole on the left and right sides of described diffuser (2).
5, microalgae cultivation accelerator according to claim 1 and 2, it is characterized in that: the top margin of described side plate (b) and front and back retaining plate (a) all is higher than 10 centimetres of the top margins of grid plate (c), 10 centimetres on the base of retaining plate (a) and grid plate (c) before and after the base of side plate (b) is higher than.
6, microalgae cultivation accelerator according to claim 1 and 2 is characterized in that: described flowing water well (1) and diffuser (2) are made by synthetic glass.
7, microalgae cultivation accelerator according to claim 3 is characterized in that: described flowing water well (1) and diffuser (2) are made by synthetic glass.
8, microalgae cultivation accelerator according to claim 4 is characterized in that: described flowing water well (1) and diffuser (2) are made by synthetic glass.
9, microalgae cultivation accelerator according to claim 5 is characterized in that: described flowing water well (1) and diffuser (2) are made by synthetic glass.
10, microalgae cultivation accelerator according to claim 6 is characterized in that: described flowing water well (1) and diffuser (2) are made by synthetic glass.
CN200910101170A 2009-07-24 2009-07-24 Microalgae cultivation accelerator Pending CN101613659A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104803A (en) * 1988-03-03 1992-04-14 Martek Corporation Photobioreactor
DE10036159A1 (en) * 2000-07-25 2002-02-14 Fraunhofer Ges Forschung Apparatus for cell cultivation is a micro-titration plate with recesses, to hold the solution with micro-organisms, and a gas feed tube to the solution in the cultivating plate recesses
CN1880442A (en) * 2006-05-15 2006-12-20 华东理工大学 Multi-section flat type photo-bioreactor
CN1982432A (en) * 2005-12-12 2007-06-20 中国科学院过程工程研究所 Carbon compensator for large-scale culturing micro-algae, its utilization and use
CN201485462U (en) * 2009-07-24 2010-05-26 浙江省海洋水产养殖研究所 Accelerator for culturing microalgae

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104803A (en) * 1988-03-03 1992-04-14 Martek Corporation Photobioreactor
DE10036159A1 (en) * 2000-07-25 2002-02-14 Fraunhofer Ges Forschung Apparatus for cell cultivation is a micro-titration plate with recesses, to hold the solution with micro-organisms, and a gas feed tube to the solution in the cultivating plate recesses
CN1982432A (en) * 2005-12-12 2007-06-20 中国科学院过程工程研究所 Carbon compensator for large-scale culturing micro-algae, its utilization and use
CN1880442A (en) * 2006-05-15 2006-12-20 华东理工大学 Multi-section flat type photo-bioreactor
CN201485462U (en) * 2009-07-24 2010-05-26 浙江省海洋水产养殖研究所 Accelerator for culturing microalgae

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Title
林晨等: "一种新型多节隔板-平板式光生物反应器的数值和实验研究", 《高校化学工程学报》 *
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Application publication date: 20091230