Oplegnathus fasciatus parent fish rearing and mating system
Technical field
The invention belongs to aquaculture field, is that the gonad development to marine fishes oplegnathus fasciatus parent population carries out artificial regulatory specifically, makes it ripe natural spawning, the artificial breeding technique of incubating oosperm and son, postlarva.
Background technology
Oplegnathus fasciatus be warm band along near sea fishes, happiness inhabits the sea area that cay, gravel, the shellfish algae of the 10-100m depth of water grow thickly; Juvenile fish summer-autumn along with marine alga drifts about.Carnivorous, tooth is sharp keen, can bite hard shell such as shellfish or sea urchin into pieces.The age at sexual maturity of oplegnathus fasciatus was 3 ages, and breeding period is many to belong to reproduction type spring and summer when spring, water temperature rose, and monthlyly had breeding individual from south to north May to 8.18-28 ℃ of natural spawning water temperature, 21-24 ℃ of spawning period water temperature.The gonad development characteristics are: ripe in batches, repeatedly lay eggs.Oplegnathus fasciatus subcutaneous containing enriched colloid, the mouthfeel uniqueness, and fine figure is called " fish of illusion " by people, the name of " king that rock angles " is also arranged, and fished deeply fan and consumer's favor is a kind of seafood fish with higher edible and ornamental value.Because oplegnathus fasciatus does not have the obvious best fishing period, output is little, and is native country nature kind, the resources development, and its breed has a extensive future.
The stable supply of the technological progress of artificial propagation and breed seed is the key of marine culture and stock enhancement industry sustainable development, and the stable supply of oplegnathus fasciatus seed will already be injected life for China's marine fish culture.Oplegnathus fasciatus is suitable for industrial aquaculture, pond and marine cage culture system, can adopt marine net cage, pond and the indoor aquaculture model that combines in the north, and at sea net cage is cultured in south.The training seedling technology of oplegnathus fasciatus artificial propagation and son, postlarva is the technical foundation that this kind is propagated artificially.
Summary of the invention
The object of the present invention is to provide parent fish rearing of marine fishes oplegnathus fasciatus and artificial breeding method.
For achieving the above object, the technical scheme taked of the present invention comprises:
Parent population is carried out prenatal nutrition strengthen and environment conditioning, impel its natural spawning, obtain fertilized egg; Incubating oosperm; Young, postlarva is cultivated; The fortification of open-mouthed bait and seed rearing phase series bait and throwing something and feeding.
Above-described concrete scheme is:
(1) selection adult fish in three ages is as parent population, and the bright sand lance of ice of throwing something and feeding adds accounting for bait weight 3-5%Vc, 1-2%V
EWith 5-8% marine fish compound premix (as: Sha Menhui be full of animal Science and Technology Ltd. produce " favour is full of " board marine fish compound premix), the secondary (8:00 and 16:00) of throwing something and feeding day.Environment conditioning is meant light control parent fish pond surface light photograph, and intensity of illumination is 60-700Lux, and light application time is the long illumination of 12L/12D-16L/8D, and water temperature 21-24 ℃, a day quantity of exchanged water is a 3-5 full dose.
(2) adopt the spilling water method to collect fertilized egg, the incubating oosperm condition is water temperature 21-24 ℃, and salinity 28-32 ‰, and pH value 8.0-8.2 is hatched under little venting condition, and promptly every cubic metre of water body is put a gas stone, and aeration quantity is 10-20L/m per hour
3
(3) young, postlarva breeding condition is water temperature 21-24 ℃, and salinity 28-32 ‰, pH value 8.0-8.2, and aeration quantity is by 20L/m per hour
3Increase to per hour 60L/m gradually
3, quantity of exchanged water is by being increased to every day 200% every day 10% gradually.Training seedling whole process using wheel animalcule → artemia nauplii → fish gruel.Open-mouthed bait is the SS type wheel animalcule or the shellfish young.Wheel animalcule and artemia nauplii all use nutrition fortifier (as: the Kang Ke A that Qingdao Samuels Industrial ﹠ Commercial Co., Ltd. produces) to strengthen, per 1,000,000 wheel animalcules restrain Kang Ke A with 0.16-0.20, per 100,000 artemia naupliis restrain Kang Ke A with 0.1-0.2, the ratio that adds a premium on currency in 20 gram Kang Ke A evenly stirs 2 times with agitator, filtering the back with 200 mesh sieve thin,tough silk uses, strengthen after 12 hours, row is thrown something and fed again.Fry grows during throw something and feed in early days wheel animalcule and the artemia nauplii, adds marine chlorella, and chlorella density keeps 80-120 ten thousand cell/ml.The program of throwing something and feeding: the 3-25 days SS type wheel animalcules of throwing something and feeding in hatching back, keep 8-10/ml by the density of throwing something and feeding 20-40/ml in the water body; The 18-35 days artemia naupliis of throwing something and feeding in hatching back, the density of throwing something and feeding 10-20/ml, keeping density in the water is 3-5/ml; Hatched back 28 days, and began to tame the fish gruel of ingesting, feeding volume is decided on the fry situation of ingesting, and through 7-9 days, can be domesticated for the fish gruel fully, can throw something and feed with the fry growth ever since and ice bright sand lance than coarsegrain.
The present invention has following advantage:
1, the present invention regulates and control the parent population synchronous spawning, can be in a planned way by row's production of growing seedlings;
2, the present invention with SS type wheel animalcule as open-mouthed bait, the weed survival rate height;
3, the present invention adds 80-120 ten thousand cell/ml marine chlorellas, and seed grows normally, and abnormal rate is low;
4, the present invention can carry out artificial breeding production in Various Seasonal under the artificial regulatory condition;
5, the present invention is suitable for oplegnathus fasciatus seed industrialization production.
The present invention is by the artificial regulatory of oplegnathus fasciatus parent population prenatal nutrition and environment, impels gonadal maturation, makes it the techniqueflow of natural spawning and incubating oosperm and son, postlarva artificial culture.Oplegnathus fasciatus parent fish rearing of the present invention and raising technology are that China's marine fish culture already increases another new breed variety and established technical foundation, and the industrialization development of oplegnathus fasciatus is had significance.
Embodiment
Embodiment 1
One, method:
(1) parent population source: select three age adult fish as parent population, at the beginning of 2004 5 months, it is complete to select the bodily form from culture adult fish, color and luster is normal, and healthy active, individuality 76 tails of body weight more than 0.5kg use as parent population, female: hero is 22: 54, carries out antenatal reinforced cultivating.
(2) parent fish rearing: parent fish pond is 1.8m * 1.8m * 1.2m cement pit; Extract nature seawater, through precipitation, carry out flowing water and cultivate, a day internal circulating load is a 3-5 full dose; The implementation fortification was raised after parent population went into the pond, and the bright sand lance of ice of throwing something and feeding adds accounting for bait weight 3%Vc, 1%V
EWith 5% marine fish compound premix (Sha Menhui be full of animal Science and Technology Ltd. produce " favour is full of " board marine fish compound premix), the secondary (8:00 and 16:00) of throwing something and feeding day; Adopt light control parent fish pond surface light photograph fully, intensity of illumination 60-400Lux transits to the long illumination of 16L/8D by natural lighting.
(3) adopt ovum: fertilized egg is collected with the spilling water method; Collect the ovum net cage in 15:00 setting every day, collect fertilized egg about 8:00 next day; Separate with the 1000ml graduated cylinder every day, calculates total ovum amount and come-up ovum amount.
(4) envirment factor: parent population is between the egg-laying period, and except that illumination, other envirment factor is nature, and water temperature 19-27 ℃, salinity about 30 ‰, pH value about 8.2.
(5) incubating oosperm: incubation condition is water temperature 21-24 ℃, salinity about 30 ‰, and pH value 8.0-8.2, little inflation, promptly every cubic metre of water body is put a gas stone, and aeration quantity is 20L/m per hour
3
(6) young, postlarva is cultivated: breeding condition is water temperature 21-24 ℃, salinity about 30 ‰, and pH value 8.0-8.2, aeration quantity is changed from small to big, and aeration quantity is by 20L/m per hour
3Increase to per hour 60L/m gradually
3, quantity of exchanged water increases gradually, and quantity of exchanged water is by being increased to every day 200% every day 10% gradually; Training seedling whole process using wheel animalcule → artemia nauplii → fish gruel; Open-mouthed bait is a SS type wheel animalcule; Wheel animalcule and artemia nauplii are all used nutrition fortifier (the Kang Ke A that Qingdao Samuels Industrial ﹠ Commercial Co., Ltd. produces), the ratio that adds a premium on currency in 20 gram Kang Ke A evenly stirs 2 times with agitator, filtering the back with 200 mesh sieve thin,tough silk uses, strengthen after 12 hours, row is thrown something and fed again, restrains Kang Ke A by per 1,000,000 wheel animalcules with 0.20; Per 100,000 artemia naupliis restrain Kang Ke A with 0.2; Fry grows during throw something and feed in early days wheel animalcule and the artemia nauplii, adds marine chlorella, and chlorella density keeps 80-120 ten thousand cell/ml.The program of throwing something and feeding: the 3-25 days SS type wheel animalcules of throwing something and feeding in hatching back, 20/ml of the density of throwing something and feeding keeps 8-10/ml in the water body; The 18-35 days artemia naupliis of throwing something and feeding in hatching back, 10/ml of the density of throwing something and feeding, keeping density in the water is 3-5/ml; Hatched back 28 days, and began to tame the fish gruel of ingesting, feeding volume is decided on the fry situation of ingesting, and through 7-9 days, can be domesticated for the fish gruel fully, can throw something and feed with the fry growth ever since and ice bright sand lance than coarsegrain.
Two, result:
Parent population is from 9-August 6 June, and natural spawning is 59 days continuously; Total egg laying amount 1807.4 * 10
4Grain, come-up ovum 1225 * 10
4Grain, floating ovum rate 67.78%; Wherein during 15-July 15 June, common property ovum 1059.8 * 10
4Grain, come-up ovum 868 * 10
4Grain, floating ovum rate 81.90%.
July 6 was collected 280,000 in fertilized egg during 8:00, put into water body 25m
3Hatching cultivate that the pond is hatched and young, postlarva is cultivated, cultivate tail surplus the above fry 20,000 of 50mm.