CN1675993A - Nanjing fairy shrimp egg proliferating method - Google Patents
Nanjing fairy shrimp egg proliferating method Download PDFInfo
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- CN1675993A CN1675993A CNA2004100173774A CN200410017377A CN1675993A CN 1675993 A CN1675993 A CN 1675993A CN A2004100173774 A CNA2004100173774 A CN A2004100173774A CN 200410017377 A CN200410017377 A CN 200410017377A CN 1675993 A CN1675993 A CN 1675993A
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Abstract
The present invention relates to a multiplication method of Nanjing fairy shrimp ova, said method includes the following steps: incubation of fairy shrimp ova, artificial multiplication and collection of fairy shrimp ova.
Description
Technical field
The present invention relates to the enrichment procedure of the animality live bait in the biological field, relate in particular to the enrichment procedure of a kind of Nanjing fairy shrimp worm's ovum.
Background technology
Present most aquatic livestock is grown seedlings and all needs to use the animality live bait, and wherein halogen worm (seawater fairy shrimp) is a kind of application animality live bait more widely.Along with the breakthrough of the extensive artificial breeding technique of new, special products of famous brands, of extra fine quality aquatic livestock, fish shrimp crab aquaculture industry thriving increases day by day to the requirement of animality live bait.Because the minimizing day by day of artemia cysts resource has directly influenced the flourish of some aquatic products artificial breeding and aquaculture.In the fresh water field, particularly the special kind of some fresh water names---producing as seeds such as pet fish, ferociousness fish and shrimps all is bait with the zooplankton, and halogen worm survival period in fresh water is short, and price is high again, and is not suitable for the seed production of the famous and precious kind of fresh water.
Nanjing fairy shrimp (Chirocephalus nankinensis (shen)) is the distinctive a kind of fresh water Branchiopoda crustacean of China, belongs to Crustachia, Anostraca, fairy shrimp section, good year Eimeria.Former Nanjing, Hangzhou one band of being distributed in moves in the fresh water, is the shrimp shape small animal of seasonality appearance.But because the transition of natural environmental condition, the original natural distribution point of Nanjing fairy shrimp reduces day by day.Since the Nanjing fairy shrimp have be of high nutritive value, the polypide size is agreeable to the taste, characteristics such as polluted water not.It can compare favourably with the halogen worm as live bait, and its nauplius can be applicable to seed rearings such as famous and precious aquatic products and aquatic animal for display, and the young and adult can be applicable to the aquatic animal of different developmental phases, can improve survival rate, reduces and grows seedlings or aquaculture cost.China is taken up in order of priority " biology of Nanjing fairy shrimp and propagation are utilized " carried out research, and obtained some progress, but concrete multiplication technique did not appear in the newspapers from 1973.At present, the processing of Nanjing fairy shrimp worm's ovum, storage, hatching adopt with collected ovum through multistage sieve dry after, air-dry preservation, worm's ovum adds the method for water inflation hatching, the worm's ovum shelf-life is short, the egg hatch rate is low and extremely asynchronous.The rich water that adopts is cultivated Nanjing fairy shrimp multiplication technique, is subjected to the influence of envirment factor bigger, often causes output not high, unstable because of the undersupply of feed and water quality are difficult to control, even does not have output.Thereby had a strong impact on of the development and utilization of Nanjing fairy shrimp as live bait.
Summary of the invention
The object of the present invention is to provide the enrichment procedure of a kind of Nanjing fairy shrimp worm's ovum.
The enrichment procedure of a kind of Nanjing of the present invention fairy shrimp worm's ovum, comprise the collection of hatching, artificial fecundation and the worm's ovum of worm's ovum, at first worm's ovum being put into aqueous slkali during hatching soaked 48 hours~72 hours, then the worm's ovum that soaked with alkali is put into water, uninterrupted inflation and control hatching water temperature are 15 ℃~20 ℃, stop inflation behind the egg hatch, carry out separating of the young and chorion; The young that hatches is put into the pond put propagation in a suitable place to breed, and uninterrupted inflation, 5 ℃~25 ℃ of control water temperatures, and the feed of manually throwing something and feeding; When fairy shrimp begins to lay eggs, collect worm's ovum; Described aqueous slkali is a sodium hydroxide solution; In multiplicative stage, 15 ℃~20 ℃ of control water temperatures; In multiplicative stage, used feed is a soya-bean milk; When being lower than 15cm, changes water transparency water; The collection of worm's ovum after the Nanjing fairy shrimp lays eggs, with the flushing of worm's ovum water and successively after 30 orders, 60 orders, 80 orders sieve, use common detergent immersion, after rinsing, ovum is put into bucket add water and draw evenly, remove the husky mud and the floating thing that sink to the bottom, soak only with clear tongue again, after rinsing is clean, get final product through dewatering, drying.
The preservation of worm's ovum is at room temperature done the Tibetan in the venting bags, also can keep in Dark Place in water, and storage life can reach 10 years in water, all right in addition low temperature or stored frozen, and such storage life is longer.
The enrichment procedure of a kind of Nanjing of the present invention fairy shrimp worm's ovum, solved the few problem of occurring in nature Nanjing fairy shrimp amount, and in the artificial culture process egg hatch rate not high, hatch nonsynchronous problem, for the application of Nanjing fairy shrimp in freshwater aquiculture provides the foundation.
Embodiment
Embodiment 1: the enrichment procedure of Nanjing of the present invention fairy shrimp worm's ovum comprises the hatching of worm's ovum, the artificial fecundation of polypide and the collection of worm's ovum.The hatching of worm's ovum: get wet ovum 100g and put into the immersion of NaOH solution after 48 hours, water is clean with the ovum rinsing.It is 12 liters bucket that the worm's ovum of handling is put into volume, adds 9 liters in water, and water temperature is controlled between 15-20 ℃, under the condition that light and uninterrupted inflation are arranged, promptly hatches a large amount of naupliuss through 48 hours.Stop inflation, leave standstill a moment, unhatched worm's ovum and chorion sink rapidly, and nauplius suspends in water, and nauplius is connected water pour out.Unhatched ovum adds can continue the inflation hatching behind the water, gets one time nauplius in per 24 hours.So repeat 4-5 days, the egg hatch rate reaches more than 70%.Remaining unhatched worm's ovum can repeat to continue the inflation hatching after the alkaline treatment.
Place cement pit to carry out artificial fecundation the young that hatches, the volume of this cement pit is 1.95X1.65X0.5 (depth of water) M
3By 600,000/m
3Density is put in a suitable place to breed, uninterruptedly inflation in whole breeding.Put the back in a suitable place to breed and began to throw in feed on the 3rd day, used feed is a soya-bean milk in the present embodiment, carries out full pool spilling head, and the throwing forage volume is raised the back transparency with throwing and is controlled at about 20cm, and throwing is raised the back and deepened water level 3-5cm every day, up to peak level 50cm.Along with the growth of polypide, when water transparency is lower than 15cm, need timely soil pick-up, the new water of annotating, will pull water body top layer foam and epistasis every day out.Carry out the collection of worm's ovum when laying eggs Deng the Nanjing fairy shrimp.
During collection worm's ovum and dirt are collected together, to from cement pit, wash successively after 30 orders, 60 orders, 80 orders sieve by the ovum water of collected band foreign material, use common detergent immersion, after rinsing, ovum is put into bucket add water and draw evenly, (this step will be carried out repeatedly to remove the husky mud that sinks to the bottom and floating thing, until obtaining clean ovum), only soak with clear tongue, rinsing is clean again, can obtain clean worm's ovum through dewatering, drying.Obtain worm's ovum 0.32kg, reduced unit's water body output is respectively 0.2kg/m
3The worm's ovum that obtains is done the Tibetan with venting bags.
Claims (6)
1, the enrichment procedure of a kind of Nanjing fairy shrimp worm's ovum, comprise the collection of hatching, artificial fecundation and the worm's ovum of worm's ovum, at first worm's ovum being put into aqueous slkali when it is characterized in that hatching soaked 48 hours~72 hours, then the worm's ovum that soaked with alkali is put into water, 15 ℃~20 ℃ of uninterrupted inflation and control hatching water temperatures, stop inflation behind the egg hatch, carry out separating of the young and chorion; The young that hatches is put into the pond put propagation in a suitable place to breed, and uninterrupted inflation, 5 ℃~25 ℃ of control water temperatures, and the feed of manually throwing something and feeding; When fairy shrimp begins to lay eggs, collect worm's ovum.
2, the enrichment procedure of a kind of Nanjing according to claim 1 fairy shrimp worm's ovum is characterized in that described aqueous slkali is a sodium hydroxide solution.
3, the enrichment procedure of a kind of Nanjing according to claim 1 fairy shrimp worm's ovum is characterized in that in the multiplicative stage, 15 ℃~20 ℃ of control water temperatures.
4, the enrichment procedure of a kind of Nanjing according to claim 1 fairy shrimp worm's ovum is characterized in that in the multiplicative stage that used feed is a soya-bean milk.
5, the enrichment procedure of a kind of Nanjing according to claim 1 fairy shrimp worm's ovum changes water when water transparency is lower than 15cm when it is characterized in that putting in a suitable place to breed propagation.
6, the enrichment procedure of a kind of Nanjing according to claim 1 fairy shrimp worm's ovum, it is characterized in that the collection of worm's ovum, with the flushing of worm's ovum water and successively after 30 orders, 60 orders, 80 orders sieve, use common detergent immersion, after rinsing, ovum is put into bucket add water and draw even, remove the husky mud and the floating thing that sink to the bottom, only soak with clear tongue again, after rinsing is clean, get final product through dewatering, drying.
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CNB2004100173774A CN1312988C (en) | 2004-04-01 | 2004-04-01 | Nanjing fairy shrimp egg proliferating method |
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CNB2004100173774A CN1312988C (en) | 2004-04-01 | 2004-04-01 | Nanjing fairy shrimp egg proliferating method |
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CN1312988C CN1312988C (en) | 2007-05-02 |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102499191A (en) * | 2011-11-28 | 2012-06-20 | 广东恒兴饲料实业股份有限公司 | Artemia separation method and device |
CN102742735A (en) * | 2012-05-08 | 2012-10-24 | 山西农业大学 | Branchinella kugenumaensis feed and preparation method thereof |
CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
CN104823882A (en) * | 2015-04-09 | 2015-08-12 | 中国水产科学研究院珠江水产研究所 | Golden apple snail egg mass scattering method |
CN105684970A (en) * | 2016-02-23 | 2016-06-22 | 珠海利洋苗种繁育有限公司 | Production method for fairy shrimp nauplii |
CN106417188A (en) * | 2016-09-22 | 2017-02-22 | 连云港众利水产养殖有限公司 | Method for inhibiting filamentous bacteria in hatching chamber of shelled artemia spawn |
CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
CN110754402A (en) * | 2019-12-09 | 2020-02-07 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
-
2004
- 2004-04-01 CN CNB2004100173774A patent/CN1312988C/en not_active Expired - Fee Related
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102499191A (en) * | 2011-11-28 | 2012-06-20 | 广东恒兴饲料实业股份有限公司 | Artemia separation method and device |
CN102499191B (en) * | 2011-11-28 | 2014-02-05 | 广东恒兴饲料实业股份有限公司 | Artemia separation device |
CN102742735A (en) * | 2012-05-08 | 2012-10-24 | 山西农业大学 | Branchinella kugenumaensis feed and preparation method thereof |
CN103478081A (en) * | 2013-08-26 | 2014-01-01 | 李坚 | Indoor high-density cultivation method of artemia |
CN104186431A (en) * | 2014-08-20 | 2014-12-10 | 天津海友佳音生物科技股份有限公司 | High-density artemia breeding method with single-cell protein single-step food chain utilized |
CN104823882A (en) * | 2015-04-09 | 2015-08-12 | 中国水产科学研究院珠江水产研究所 | Golden apple snail egg mass scattering method |
CN104823882B (en) * | 2015-04-09 | 2017-08-01 | 中国水产科学研究院珠江水产研究所 | A kind of method of scattered Pomacea canaliculata pieces of an egg |
CN105684970A (en) * | 2016-02-23 | 2016-06-22 | 珠海利洋苗种繁育有限公司 | Production method for fairy shrimp nauplii |
CN106417188A (en) * | 2016-09-22 | 2017-02-22 | 连云港众利水产养殖有限公司 | Method for inhibiting filamentous bacteria in hatching chamber of shelled artemia spawn |
CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
CN110754402A (en) * | 2019-12-09 | 2020-02-07 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
CN110754402B (en) * | 2019-12-09 | 2021-10-26 | 海南晨海水产有限公司 | Indoor high-density cultivation method for artemia |
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