CN1751666A - Method for artificial breeding mudskipper - Google Patents
Method for artificial breeding mudskipper Download PDFInfo
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- CN1751666A CN1751666A CNA2005101183617A CN200510118361A CN1751666A CN 1751666 A CN1751666 A CN 1751666A CN A2005101183617 A CNA2005101183617 A CN A2005101183617A CN 200510118361 A CN200510118361 A CN 200510118361A CN 1751666 A CN1751666 A CN 1751666A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
An artificial reproduction method for bluespotted mudhopper includes such steps as providing harmone LHRH-A3 and/or HCG, injecting the harmone into the parent bluespotted mudhopper, using the sex pheromone to induce the mating and ovipositing of parents in oviscapt, artificial fertilization by semi-drying method, washing with clean seawater and innoculation.
Description
Technical field
The present invention relates to the artificial fecundation method of a kind of Fish, especially relate to a kind of artificial fecundation method of mudskipper.
Background technology
Mudskipper (Boleophthalmus pectinirostris) belongs to Perciformes, and (mudskipper belongs to (Boleophthalmus) for Perciformes) ， Acenthogobius flavimanus suborder (Gobiodei), mudskipper section (Periophthalmidae).Mudskipper is distributed in China, Korea, Japan, Burma and the Malay Peninsula, mainly is distributed in Jiangsu, Zhejiang, Fujian, Taiwan, Guangdong and ALONG GUANGXI COAST in China.Mudskipper inhabits Intertidal zone, bay, river mouth mud beach and mangrove area, belong to the warm wide temperature eurysalinity of the water Fish that live in caves, its juvenile fish and the adult fish benthic diatom that mainly ingests has that food chain is short, disease is few, survival rate is high, aquaculture cost is low, characteristics such as convenient management, the free from environmental pollution and anti-transportation of live fish.Mudskipper meat flavour deliciousness, nutritious, have the effect of nourishing and fit keeping function, contain 16 common seed amino acids, wherein 8 kinds is human body essential amino acid (Wang Jun, Su Yongquan, Xiamen University's journal (natural science edition), 1994,33 (1): 96-99), be subjected to liking of the coastal masses deeply.Market demand was very big in recent years, was a kind of mudflat aquaculture economic fish with market prospect.
The mudskipper seed that China cultures always is picks up from natural sea area beach.But because coastal beach is enclosed and cultivated in a large number over nearly more than 20 years, the habitat area of mudskipper constantly reduces, reason such as add environmental pollution and excessively capture, natural waters mudskipper seeding-growth quantity reduces year by year, the strain price goes up year by year, the disparities between supply and demand of seed are outstanding, and aquaculture cost increases, and has seriously hindered the development of mudskipper aquaculture.
In order to solve the seed problem, China Taiwan is early than carrying out mudskipper artificial propagation and seedling growth test the seventies in 20th century, interrupt later on research (Liao Yijiu, Chinese Aquatic product (Taiwan), 1986,398:23-27).Zhang Qiyong equals to carry out the eighties in 20th century mudskipper artificial propagation and the research of growing seedlings, utilize parent fish artificial insemination after hormone is hastened parturition of Intertidal zone, cultivate the seed (Zhang Qiyong that 7000 tails reach stocking size, Hong Wanshu, Dai Qingnian etc., Xiamen University's journal (natural science edition), 1987,26 (3): 366-373; Zhang Qiyong, Hong Wanshu, Dai Qingnian etc., Aquaculture, 1989,83:375-385).Early 1990s, Ni Yong etc. carry out the mudskipper artificial propagation and the intermediate experiment of growing seedlings, and cultivate the fry of tail more than 7000 (Ni Yong, Zhang Qiyong, Chen Defu etc., sea fishery, 1993,15 (2): 56-62).In 20 years, the artificial propagation and the seedling-raising technique of mudskipper remain at the same level substantially, fail to obtain tangible progress in the past.Its main cause is still to have some problems at aspects such as parent fish selection, the method for hastening parturition, artificial insemination, incubating oosperm and induced spawnings.
Summary of the invention
The objective of the invention is to artificial propagation and the existing problem of seedling-raising technique at existing mudskipper, a kind of physiology and ecosystem characterization of utilizing the mudskipper reproduction is provided, adopt the method for endocrine regulation and simulation reproduction environment, improve the artificial fecundation method of the rate of accelerating the ripening, egg laying amount and the rate of fertilization of mudskipper parent fish.
Step of the present invention is:
1) parent fish is accelerated the ripening: choose the male parent fish that abdominal part expands, the circular evagination of urogenital papilla is cherry female parent fish and urogenital papilla projection triangular in shape at breeding season, the employing injection of hormone accelerates the ripening, and said hormone is luteotropin releasing hormone d-ala analog (LHRH-A
3) and human chorionic gonadotropin (HCG) in a kind of or two kinds of mixture, per injection dosage is LHRH-A
30.5～1 μ g+HCG 100～150IU/ tail, or LHRH-A
31.0～2.0 μ g/ tails, or HCG 100～150IU/ tail, female, male parent fish Isodose, the injection site is the pectoral fin base portion, it is respond well to accelerate the ripening;
2) sex pheromone induces parent fish to lay eggs: the parent fish of accelerating the ripening through hormone, by female: spawning pond is put in male=pairing in 1: 1, the pipeline of laying eggs is established in the spawning pond bottom, hang sex steroid hormone 17 α-hydroxyprogesterone (17 α-P) or 17 α at the pipeline infantile tic of laying eggs, (17 α, 20 β-P) wait sex pheromone induce parent fish to enter to lay eggs mate and oviposit in the pipeline to the two hydroxyprogesterones of 20 β, realize the reproduction synchronization, improved the rate of fertilization of spawning rate, egg laying amount and the mature egg of raun;
3) artificial insemination: parent fish adopts semidry method to carry out artificial insemination after hormone accelerates the ripening, and extrudes female fish egg before the artificial insemination earlier and checks its Maturity, chooses the mature egg with following feature and is used for artificial insemination: (1) ovum is circular and transparent; (2) heavy wool ball and concentrated shinny; (3) the adhesive filament prosperity in the egg membrane outside; Dissect raun during insemination and take out ovary, tear ovary membrane, disperse ovum; Dissect milter simultaneously and take out spermary and Seminal vesicle capsule, be cut into small pieces, place in the mortar and grind, add a little sea-water activated sperm, then seminal fluid is watered on the ovum group, stirring allows it fully be fertilized, and then with clean sea water washing 3～4 times, moves in the incubation vessel and hatches.
The present invention is by selecting parent fish hasten parturition required hormone kind, appropriate dosage and the method for effectively hastening parturition, utilize the physiology and the ecosystem characterization of mudskipper reproduction simultaneously, selection can obtain the mature egg of high rate of fertilization, and adopt endocrine regulation and simulate the method for reproduction environment, induce methods such as parent fish mate and oviposit and employing artificial fertilization with sex pheromone, improve the rate of accelerating the ripening, egg laying amount and the rate of fertilization of parent fish greatly, made the mudskipper artificial propagation techniques obtain breakthrough.
The specific embodiment
Below by embodiment the present invention is elaborated.
Embodiment 1
That breeding season is chosen is healthy and strong active, the breed parent fish of gonad development to the IV phase.Parent's fish length 10.5～13.0cm, body weight 17.3～32.0g.Female parent fish abdominal part expands, and the circular evagination of urogenital papilla is cerise; Male parent fish urogenital papilla projection triangular in shape.The parent fish age was 1～2 age.Spawning pond area 10m
2/ mouthful, the dark 1.5m in pond, at the bottom of the pond and pool wall lay black ceramic tiles, avoid the parent fish scratch.The pond face keeps low light environment with the shading of sunshade net.The pipeline of laying eggs is made by ceramic material, the long 50cm of pipeline, wide 9.5cm, high 7.5cm.Be divided into 2 parts up and down, first half inner wall coarse helps germ cell adhering to; The Lower Half inwall is coated with glaze, helps activity and the mate and oviposit of parent fish.Pipeline up and down 2 parts can separate or make up, the convenient inspection parent fish situation of laying eggs.
1, parent fish is accelerated the ripening: divide 3 groups to be the contrast experiment, every group of raun 30 tails.The 1st group with LHRH-A
3And female, the male parent fish of two kinds of hormone hybrid injections of HCG (available from Ningbo Hormone product Co., Ltd.), thoracic cavity injection at twice, per injection dosage is LHRH-A
31 μ g+HCG 100IU, female, male parent fish injection Isodose, double injection is 48h at interval.The 2nd group and the 3rd group of independent LH injection RH-A of difference
31 μ g or HCG100IU only injects 1 time.The result shows (seeing Table 1), adopts the method for hormotone and 2 injections, and its rate of accelerating the ripening is all injected for 1 time apparently higher than single hormone of planting.
The different injecting methods of table 1 are to the effect of accelerating the ripening of mudskipper raun
| Group | Raun mantissa | Injection hormone and dosage (tail) | Ripe mantissa | The rate of accelerating the ripening (%) | |
| The 1st time | The 2nd time | ||||
| 1 | 30 | LHRH-A 31μg+HCG 100IU | LHRH-A 31μg+HCG 100IU | 18 | 60.0 |
| 2 | 30 | LHRH-A 31μg | 0 | 11 | 36.7 |
| 3 | 30 | HCG 100IU | 0 | 10 | 33.3 |
2, sex pheromone induced spawning: 17 α-P and 17 α, 20 β-P (available from U.S. Sigma company) are through dissolve with methanol, get 100 μ g pack into (2mL) in the plastic tube respectively, after drying up, nitrogen adds normal saline to 1mL, a little Cotton Gossypii of mouth of pipe plug, hang at the pipeline first half inner surface of laying eggs, in the water body of hormone in Cotton Gossypii is penetrated into pipeline.
Through the parent fish that hormone accelerates the ripening, put into spawning pond by female, pairing in male 1: 1.Parent fish goes into the situation of laying eggs that 48h behind the pond checks the pipeline in-laws fish that lays eggs, and calculates egg laying amount with area-method, calculates rate of fertilization fetal development to gastrul stage.The spawning pond top covers double-deck sunshade net during the induced spawning, keeps low light environment, and water body is not inflated, and water temperature 25.0-27.0 ℃, salinity 15-20.The result shows (seeing Table 2), hangs the experimental group of sex pheromone in the pipeline, and its pipeline spawning rate, total egg laying amount and average rate of fertilization all are significantly higher than matched group.
Table 2 sex pheromone is induced the mudskipper result of laying eggs
| Group | The ♀ ♂ of parent fish mantissa | Place the pipeline number | The sex pheromone kind | The pipeline number of laying eggs | Pipeline spawning rate (%) | Total egg laying amount (grain) | Average rate of fertilization (%) |
| 1 | 20 20 | 20 | 17α-P | 8 | 40.0 | 1500 | 81 |
| 2 | 20 20 | 20 | 17α，20β-P | 7 | 35.0 | 1800 | 85 |
| 3 | 2020 | 20 | Matched group | 2 | 10.0 | 210 | 10 |
3, artificial insemination: after parent fish injection hormone accelerated the ripening, the raun abdominal part expanded, and just can select the female parent fish that the ovum growth meets the fertilization standard this moment and be used for artificial insemination.At first extrude female fish egg and check its Maturity,, just begin to carry out artificial insemination if that ovum has is circular and transparent, heavy wool ball and concentrate features such as shinny and adhesive filament prosperity.
Artificial insemination operational approach:, choose in the culture dish (diameter 12cm) that the mature egg that meets the fertilization standard places big specification Yi Bian dissect raun; Another side is dissected ripe milter, takes out spermary and Seminal vesicle, puts into mortar, is cut into small pieces with shears, adds a little sea-water activated sperm after grinding.Sperm after activating is watered on the ovum group, stir the ovum group gently with feather then, it fully is fertilized.With clean sea water washing 2-3 time, remove unnecessary sperm and impurity behind the 3-5min, germ cell is moved in the incubation vessel hatch.
The Maturity of female fish egg is divided into 3 classes carries out artificial insemination.The 1st class is that the ovum circle is transparent, the heavy wool ball and concentrate shinny, adhesive filament is flourishing; The 2nd class is that the ovum circle is transparent, the heavy wool ball but disperse little bright, the medium prosperity of adhesive filament; The 3rd class is that the ovum circle is transparent slightly, heavy wool ball but disperse little brightly, and adhesive filament is undeveloped.The parent fish number of all kinds of ovums is 20 tails.Experimental result shows that the average rate of fertilization of the 1st class ovum is that 76.6%, the 2 class is that 42.0%, the 3 class is 4.9%.The average rate of fertilization of the 1st class ovum all is significantly higher than the 2nd class and the 3rd class.
Embodiment 2
That breeding season is chosen is healthy and strong active, the wild parent fish of gonad development to the IV phase.Parent's fish length 10.0-12.2cm, body weight 17.0-28.5g.Female parent fish abdominal part expands, and the circular evagination of urogenital papilla is cerise; Male parent fish urogenital papilla projection triangular in shape.The parent fish age is 1-2 age.Spawning pond area 5m
2/ mouthful, the dark 1.5m in pond, at the bottom of the pond and pool wall lay black ceramic tiles, avoid the parent fish scratch.The pond face keeps low light environment with the shading of sunshade net.Lay eggs pipeline with embodiment 1.
1, parent fish is accelerated the ripening: with embodiment 1, divide 3 groups to be the contrast experiment, the results are shown in Table 3, adopt the rate of accelerating the ripening of 2 injections of hormotone can reach 65%, and single rate of accelerating the ripening of planting 1 injection of hormone only is 45.0% (LHRH-A
3) or 40.0% (HCG).
The different injecting methods of table 3 are to the effect of accelerating the ripening of mudskipper raun
| Group | Raun mantissa | Injection hormone and dosage (tail) | Ripe mantissa | The rate of accelerating the ripening (%) | |
| The 1st time | The 2nd time | ||||
| 1 | 20 | LHRH-A 31μg+HCG 100IU | LHRH-A 31μg+HCG 100IU | 13 | 65.0 |
| 2 | 20 | LHRH-A 31μg | 0 | 9 | 45.0 |
| 3 | 20 | HCG 100IU | 0 | 8 | 40.0 |
2, pheromone induced spawning: 26.5～29.0 ℃ of water temperatures.Other are with embodiment 1.The results are shown in Table 4, hang 17 α-P or 17 α in the pipeline, the experimental group of 20 β-P, its pipeline spawning rate, total egg laying amount and average rate of fertilization are significantly higher than matched group respectively.
Table 4 sex pheromone is induced the mudskipper result of laying eggs
| Group | The ♀ ♂ of parent fish mantissa | Place the pipeline number | The sex pheromone kind | The pipeline number of laying eggs | Pipeline spawning rate (%) | Total egg laying amount (grain) | Average rate of fertilization (%) |
| 1 | 15 15 | 15 | 17α-P | 6 | 40.0 | 650 | 75 |
| 2 | 15 15 | 15 | 17α，20β-P | 7 | 46.7 | 1050 | 90 |
| 3 | 15 15 | 15 | Matched group | 1 | 0.7 | 80 | 15 |
3, artificial insemination is that 86.5%, the 2 class is that 52.4%, the 3 class is 6.1% with the average rate of fertilization of embodiment 1, the 1 class ovum.The average rate of fertilization of the 1st class ovum all is significantly higher than the 2nd class and the 3rd class.The parent fish number of all kinds of ovums is 25 tails.
Embodiment 3
That breeding season is chosen is healthy and strong active, the beach seining cultivation parent fish of gonad development to the IV phase.Parent's fish length 10.2-12.8cm, body weight 17.1-31.0g.Female parent fish abdominal part expands, and the circular evagination of urogenital papilla is cerise; Male parent fish urogenital papilla projection triangular in shape.The parent fish age is 1-2 age.Spawning pond area 10m
2/ mouthful, the dark 1.5m in pond, at the bottom of the pond and pool wall lay black ceramic tiles, avoid the parent fish scratch.The pond face keeps low light environment with the shading of sunshade net.Lay eggs pipeline with embodiment 1.
1, parent fish is accelerated the ripening; With embodiment 1, divide 3 groups to be the contrast experiment, the results are shown in Table 5, adopt the rate of accelerating the ripening of 2 injections of hormotone can reach 70.0%, and single rate of accelerating the ripening of planting 1 injection of hormone only is 43.3% (LHRH-A
3) or 40.0% (HCG).
The different injecting methods of table 5 are to the effect of accelerating the ripening of mudskipper raun
| Group | Raun mantissa | Injection hormone and dosage (tail) | Ripe mantissa | The rate of accelerating the ripening (%) | |
| The 1st time | The 2nd time | ||||
| 1 | 30 | LHRH-A 30.5μg+HCG 150IU | LHRH-A 31μg+HCG 100IU | 21 | 70.0 |
| 2 | 30 | LHRH-A 32μg | 0 | 13 | 43.3 |
| 3 | 30 | HCG 150IU | 0 | 12 | 40.0 |
2, sex pheromone induced spawning: 27.5～30.5 ℃ of water temperatures, other as can be seen from Table 6, hang 17 α-P or 17 α with embodiment 1 in the pipeline, the experimental group of 20 β-P, its pipeline spawning rate, total egg laying amount and average rate of fertilization are significantly higher than matched group (table 6) respectively.
Table 6 sex pheromone is induced the mudskipper result of laying eggs
| Group | The ♀ ♂ of parent fish mantissa | Place the pipeline number | The sex pheromone kind | The pipeline number of laying eggs | Pipeline spawning rate (%) | Total egg laying amount (grain) | Average rate of fertilization (%) |
| 1 | 20 20 | 20 | 17α-P | 9 | 45.0 | 1350 | 80 |
| 2 | 20 20 | 20 | 17α，20β-P | 10 | 50.0 | 1600 | 86 |
| 3 | 20 20 | 20 | Matched group | 2 | 10.0 | 180 | 20 |
3, artificial insemination: with embodiment 1.The average rate of fertilization of the 1st class ovum is that 81.4%, the 2 class is that 45.8%, the 3 class is 5.2%.The average rate of fertilization of the 1st class ovum all is significantly higher than the 2nd class and the 3rd class.The parent fish number of all kinds of ovums is 20 tails.
Claims (4)
1, the artificial fecundation method of mudskipper is characterized in that the steps include:
1) parent fish is accelerated the ripening: breeding season is chosen the male parent fish that abdominal part expands, the circular evagination of urogenital papilla is cherry female parent fish and urogenital papilla projection triangular in shape, adopts injection of hormone to accelerate the ripening, and said hormone is luteotropin releasing hormone d-ala analog LHRH-A
3With a kind of or two kinds of mixture among the human chorionic gonadotropin HCG, per injection dosage is LHRH-A
31 μ g+HCG 100IU/ tail, or LHRH-A
31.0～2.0 μ g/ tails, or HCG 100～150IU/ tail, female, male parent fish Isodose, injection site are the pectoral fin base portion;
2) sex pheromone induces parent fish to lay eggs: the parent fish of accelerating the ripening through hormone, by female: spawning pond is put in male=pairing in 1: 1, the pipeline of laying eggs is established in the spawning pond bottom, the pipeline infantile tic is hung sex steroid hormone 17 α-hydroxyprogesterone 17 α-P or 17 α laying eggs, two hydroxyprogesterone 17 α of 20 β, 20 β-P sex pheromone induce parent fish to enter to lay eggs mate and oviposit in the pipeline;
3) artificial insemination: parent fish adopts semidry method to carry out artificial insemination after hormone accelerates the ripening, and extrudes female fish egg before the artificial insemination earlier and checks its Maturity, chooses the mature egg with following feature and is used for artificial insemination: a ovum is circular and transparent; B heavy wool ball and concentrated shinny; The adhesive filament prosperity in the c egg membrane outside; Dissect raun during insemination and take out ovary, tear ovary membrane, disperse ovum; Dissect milter simultaneously and take out spermary and Seminal vesicle capsule, be cut into small pieces, place in the mortar and grind, add a little sea-water activated sperm, then seminal fluid is watered on the ovum group, stirring allows it fully be fertilized, and then with clean sea water washing 3～4 times, moves in the incubation vessel and hatches.
2, the artificial fecundation method of mudskipper as claimed in claim 1 is characterized in that at the bottom of the spawning pond pond and pool wall is laid black ceramic tiles, and the pond face keeps low light environment with the shading of sunshade net.
3, the artificial fecundation method of mudskipper as claimed in claim 1, the pipeline that it is characterized in that laying eggs is made by ceramic material, is divided into 2 parts up and down, first half inner wall coarse, the Lower Half inwall is coated with glaze.
4, the artificial fecundation method of mudskipper as claimed in claim 3, the pipeline that it is characterized in that laying eggs 2 parts up and down separate or combination.
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| CNA2005101183617A CN1751666A (en) | 2005-10-28 | 2005-10-28 | Method for artificial breeding mudskipper |
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| CNA2005101183617A CN1751666A (en) | 2005-10-28 | 2005-10-28 | Method for artificial breeding mudskipper |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585095A (en) * | 2015-01-20 | 2015-05-06 | 青岛龙宫海洋牧场生态修复工程有限公司 | Culturing method of aurelia scyphistoma |
| CN105519465A (en) * | 2015-12-23 | 2016-04-27 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Breeding method for mudskipper |
| CN107667924A (en) * | 2017-11-02 | 2018-02-09 | 成都金川田农机制造有限公司 | The cultural method of mudskipper |
| CN109479763A (en) * | 2018-10-30 | 2019-03-19 | 南宁学院 | A kind of mudskipper cultural method |
| CN109496917A (en) * | 2018-10-30 | 2019-03-22 | 南宁学院 | A kind of high-yield cultivation method of mudskipper |
| CN109511580A (en) * | 2018-10-30 | 2019-03-26 | 南宁学院 | A kind of ecological efficient cultural method of mudskipper |
-
2005
- 2005-10-28 CN CNA2005101183617A patent/CN1751666A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585095A (en) * | 2015-01-20 | 2015-05-06 | 青岛龙宫海洋牧场生态修复工程有限公司 | Culturing method of aurelia scyphistoma |
| CN105519465A (en) * | 2015-12-23 | 2016-04-27 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Breeding method for mudskipper |
| CN107667924A (en) * | 2017-11-02 | 2018-02-09 | 成都金川田农机制造有限公司 | The cultural method of mudskipper |
| CN109479763A (en) * | 2018-10-30 | 2019-03-19 | 南宁学院 | A kind of mudskipper cultural method |
| CN109496917A (en) * | 2018-10-30 | 2019-03-22 | 南宁学院 | A kind of high-yield cultivation method of mudskipper |
| CN109511580A (en) * | 2018-10-30 | 2019-03-26 | 南宁学院 | A kind of ecological efficient cultural method of mudskipper |
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