CN1560610A - 同型半胱氨酸测定方法及其试剂 - Google Patents
同型半胱氨酸测定方法及其试剂 Download PDFInfo
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- CN1560610A CN1560610A CNA2004100167896A CN200410016789A CN1560610A CN 1560610 A CN1560610 A CN 1560610A CN A2004100167896 A CNA2004100167896 A CN A2004100167896A CN 200410016789 A CN200410016789 A CN 200410016789A CN 1560610 A CN1560610 A CN 1560610A
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- Prior art keywords
- adenosine
- homocysteine
- enzyme
- methionine
- reagent
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 37
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 title abstract description 9
- 235000018417 cysteine Nutrition 0.000 title abstract description 9
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims abstract description 48
- 229960004452 methionine Drugs 0.000 claims abstract description 26
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims abstract description 24
- 229960005305 adenosine Drugs 0.000 claims abstract description 24
- 230000000694 effects Effects 0.000 claims abstract description 15
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 claims description 46
- 102000004190 Enzymes Human genes 0.000 claims description 29
- 108090000790 Enzymes Proteins 0.000 claims description 29
- 238000006243 chemical reaction Methods 0.000 claims description 21
- 108020002202 Adenosylhomocysteinase Proteins 0.000 claims description 17
- 102000005234 Adenosylhomocysteinase Human genes 0.000 claims description 16
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 16
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 16
- 229930182817 methionine Natural products 0.000 claims description 13
- 230000004044 response Effects 0.000 claims description 12
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims description 9
- 238000003556 assay Methods 0.000 claims description 9
- 210000001124 body fluid Anatomy 0.000 claims description 9
- 239000010839 body fluid Substances 0.000 claims description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 8
- 229910021529 ammonia Inorganic materials 0.000 claims description 8
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims description 8
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 claims description 6
- 102000055025 Adenosine deaminases Human genes 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 101710088194 Dehydrogenase Proteins 0.000 claims description 5
- FFFHZYDWPBMWHY-UHFFFAOYSA-N L-Homocysteine Natural products OC(=O)C(N)CCS FFFHZYDWPBMWHY-UHFFFAOYSA-N 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 101710101148 Probable 6-oxopurine nucleoside phosphorylase Proteins 0.000 claims description 4
- 102000030764 Purine-nucleoside phosphorylase Human genes 0.000 claims description 4
- 102100033220 Xanthine oxidase Human genes 0.000 claims description 4
- 108010093894 Xanthine oxidase Proteins 0.000 claims description 4
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 3
- 229930195722 L-methionine Natural products 0.000 claims description 3
- 108010007784 Methionine adenosyltransferase Proteins 0.000 claims description 3
- 102000003992 Peroxidases Human genes 0.000 claims description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 2
- 150000003838 adenosines Chemical class 0.000 claims description 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 2
- 238000006911 enzymatic reaction Methods 0.000 claims 3
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 claims 2
- 102100020925 Adenosylhomocysteinase Human genes 0.000 claims 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims 1
- 102100026115 S-adenosylmethionine synthase isoform type-1 Human genes 0.000 claims 1
- 230000003301 hydrolyzing effect Effects 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 229910001414 potassium ion Inorganic materials 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract 2
- 108091000080 Phosphotransferase Proteins 0.000 abstract 1
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- 230000002829 reductive effect Effects 0.000 description 9
- 239000005515 coenzyme Substances 0.000 description 7
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
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- 108010049351 adenosine nucleosidase Proteins 0.000 description 4
- 239000003593 chromogenic compound Substances 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 238000013016 damping Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
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- 230000009467 reduction Effects 0.000 description 3
- 238000007616 round robin method Methods 0.000 description 3
- -1 sulfhydryl compound Chemical class 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 102100036664 Adenosine deaminase Human genes 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 102000007357 Methionine adenosyltransferase Human genes 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- PTKRUDMLGIIORX-ITGWJZMWSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2r,3s,4r,5r)-5-(3-carbamoyl-4h-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate;cyclohexanamine Chemical compound NC1CCCCC1.NC1CCCCC1.NC1CCCCC1.NC1CCCCC1.C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 PTKRUDMLGIIORX-ITGWJZMWSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- MWFOPMKUGZLPQA-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methoxyanilino)propane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CCCN(CC)C1=CC=CC(OC)=C1 MWFOPMKUGZLPQA-UHFFFAOYSA-M 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- RTDXMJJSUVCSKB-RUCXOUQFSA-N (2s)-2-azanyl-4-sulfanyl-butanoic acid Chemical compound OC(=O)[C@@H](N)CCS.OC(=O)[C@@H](N)CCS RTDXMJJSUVCSKB-RUCXOUQFSA-N 0.000 description 1
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- YDBHVMTTYXWHLI-UHFFFAOYSA-N 2,4,6-tribromo-3-hydroxybenzoic acid Chemical compound OC(=O)C1=C(Br)C=C(Br)C(O)=C1Br YDBHVMTTYXWHLI-UHFFFAOYSA-N 0.000 description 1
- MSUBEYXGDSJIPS-UHFFFAOYSA-N 3-(2-methylanilino)propan-1-ol Chemical compound CC1=CC=CC=C1NCCCO MSUBEYXGDSJIPS-UHFFFAOYSA-N 0.000 description 1
- BTIDJAQNJLWPTI-UHFFFAOYSA-N 3-(n-ethyl-3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC(OC)=CC(OC)=C1 BTIDJAQNJLWPTI-UHFFFAOYSA-N 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- GKGQYWDLSLRTPJ-UHFFFAOYSA-N C(=O)O.OC=1C(=CC(=CC1Br)Br)Br Chemical compound C(=O)O.OC=1C(=CC(=CC1Br)Br)Br GKGQYWDLSLRTPJ-UHFFFAOYSA-N 0.000 description 1
- XATCVRKQPMGQFW-UHFFFAOYSA-N CCN(CC(CS(=O)(=O)O)O)C1=CC=CC=C1OC Chemical compound CCN(CC(CS(=O)(=O)O)O)C1=CC=CC=C1OC XATCVRKQPMGQFW-UHFFFAOYSA-N 0.000 description 1
- VEIZTCQKWBVPBD-UHFFFAOYSA-N CCN(CCCS(=O)(=O)O)C1=CC=CC=C1OC Chemical compound CCN(CCCS(=O)(=O)O)C1=CC=CC=C1OC VEIZTCQKWBVPBD-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 206010020608 Hypercoagulation Diseases 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 125000002015 acyclic group Chemical group 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
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- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
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- 238000007357 dehydrogenase reaction Methods 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
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- 229960002989 glutamic acid Drugs 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- 229960003786 inosine Drugs 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
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- 239000000049 pigment Substances 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- HDARHUHTZKLJET-UHFFFAOYSA-M sodium;3-(n-ethyl-3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC(OC)=CC(OC)=C1 HDARHUHTZKLJET-UHFFFAOYSA-M 0.000 description 1
- CJUDSKIRZCSXJA-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methoxyanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC=CC(OC)=C1 CJUDSKIRZCSXJA-UHFFFAOYSA-M 0.000 description 1
- IRQRBVOQGUPTLG-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 IRQRBVOQGUPTLG-UHFFFAOYSA-M 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
试剂组份 | 每升用量 | 常用每升用量范围 |
磷酸盐缓冲液,pH7.0,37℃ | 100mM | 10~300mM |
EDTA.2Na | 0.2mM | 0.1~20mM |
MgSO4 | 15mM | 0.5~100mM |
Triton X-100 | 0.1% | 0.01~5% |
DTT | 2mM | 0.1~20mM |
ATP | 80mM | 0.1~90mM |
ADA | 3ku | 0.1~200ku |
ADPS | 2mM | 0.01~20mM |
AdoHcyase | 10ku | 0.1~50ku |
甘露糖醇 | 20mM | 1~100mM |
氧化型辅酶NAD | 0.1mM | 0.01~20mM |
蛋氨酸转腺苷酶 | 15ku | 0.1~50ku |
嘌呤核苷磷酸化酶 | 2ku | 0.1~50ku |
黄嘌呤氧化酶 | 3ku | 0.1~50ku |
辣根过氧化物酶 | 2ku | 0.1~50ku |
同型半胱氨酸转甲基酶 | 4ku | 0.1~100ku |
试剂组份 | 每升用量 | 常用每升用量范围 |
磷酸盐缓冲液,pH7.0,37℃ | 100mM | 10~300mM |
EDTA.2Na | 0.2mM | 0.1~20mM |
MgSO4 | 15mM | 0.5~100mM |
Triton X-100 | 0.1% | 0.01~5% |
蛋氨酸 | 0.5mM | 0.1~90mM |
4-氨基安替比林 | 1mM |
试剂组份 | 每升用量 | 常用每升用量范围 |
磷酸盐缓冲液,pH6.5,37℃ | 150mM | 500~500mM |
EDTA.2Na | 0.5mM | 0.1~20mM |
MgSO4 | 15mM | 0.5~100mM |
DTT | 1.5mM | 0.1~20mM |
ATP | 80mM | 0.1~90mM |
ADA | 3ku | 0.1~200ku |
AdoHcyase | 10ku | 0.1~50ku |
牛血清白蛋白 | 0.2% | 0.01~10% |
α-酮戊二酸 | 7.5mM | 1~20mM |
蛋氨酸转腺苷酶 | 15ku | 0.1~100ku |
谷氨酸脱氢酶 | 5ku | 1~50ku |
同型半胱氨酸转甲基酶 | 5ku | 0.1~50ku |
试剂组份 | 每升用量 | 常用每升用量范围 |
HEPES缓冲液,pH8.3,37℃ | 50mM | 10~200mM |
EDTA.2Na | 0.5mM | 0.1~20mM |
MgSO4 | 15mM | 0.5~100mM |
蛋氨酸 | 10mM | 0.1~90mM |
甘露糖醇 | 20mM | 1~100mM |
还原型辅酶NADH | 0.8mM | 0.1~10mM |
乳酸脱氢酶 | 2ku | 0.5~20ku |
Claims (6)
Priority Applications (1)
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CNB2004100167896A CN100554948C (zh) | 2004-03-08 | 2004-03-08 | 同型半胱氨酸测定方法及其试剂 |
Publications (2)
Publication Number | Publication Date |
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CN1560610A true CN1560610A (zh) | 2005-01-05 |
CN100554948C CN100554948C (zh) | 2009-10-28 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNB2004100167896A Expired - Lifetime CN100554948C (zh) | 2004-03-08 | 2004-03-08 | 同型半胱氨酸测定方法及其试剂 |
Country Status (1)
Country | Link |
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CN (1) | CN100554948C (zh) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7097968B2 (en) | 2003-07-10 | 2006-08-29 | General Atomics | Methods and compositions for assaying homocysteine |
CN101915760A (zh) * | 2010-07-07 | 2010-12-15 | 东华大学 | 一种比色法即时检测半胱氨酸含量的方法 |
CN102243227A (zh) * | 2010-12-16 | 2011-11-16 | 浙江亚太药业股份有限公司 | 非对称二甲基精氨酸浓度的测定方法及测定试剂 |
CN102766677A (zh) * | 2012-07-31 | 2012-11-07 | 武汉生之源生物科技有限公司 | 乳酸脱氢酶检测试剂盒及其制备方法 |
US8476034B2 (en) | 2003-07-10 | 2013-07-02 | General Atomics | Methods and compositions for assaying homocysteine |
CN103913581A (zh) * | 2013-11-20 | 2014-07-09 | 天津市宝坻区人民医院 | 血清中甘油三酯的循环酶测定方法 |
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CN104630324A (zh) * | 2015-02-28 | 2015-05-20 | 北京爱必信生物技术有限公司 | 改进的同型半胱氨酸检测试剂及方法 |
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2004
- 2004-03-08 CN CNB2004100167896A patent/CN100554948C/zh not_active Expired - Lifetime
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