CN1507566A - 测试全血的方法 - Google Patents
测试全血的方法 Download PDFInfo
- Publication number
- CN1507566A CN1507566A CNA028095995A CN02809599A CN1507566A CN 1507566 A CN1507566 A CN 1507566A CN A028095995 A CNA028095995 A CN A028095995A CN 02809599 A CN02809599 A CN 02809599A CN 1507566 A CN1507566 A CN 1507566A
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- CN
- China
- Prior art keywords
- whole blood
- analyte
- reactive system
- reaction
- specific bond
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2525—Stabilizing or preserving
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
发光强度 | ||
阴性对照血清 | 平均 | 257 |
标准偏差 | 17 | |
CV(%) | 6.5% | |
阳性对照血清 | 平均 | 43035 |
标准偏差 | 1404 | |
CV(%) | 3.1% |
样品 | 在全血混合后去垢剂的浓度 | 溶血 | 磁性颗粒与反应容器壁的黏附 | 磁性颗粒的聚集 | 发光强度 | 回收比例 | |
曲通X-100 | 全血 | 1% | 存在 | 没有 | 存在 | 5130 | 42% |
吐温20 | 全血 | 1% | 没有 | 没有 | 没有 | 10620 | 87% |
吐温80 | 全血 | 1% | 没有 | 没有 | 没有 | 10260 | 85% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 2% | 没有 | 痕量 | 没有 | 10830 | 88% |
Brij78 | 全血 | 1% | 存在 | 没有 | 存在 | 5820 | 48% |
皂苷 | 全血 | 1% | 存在 | 明显 | 存在 | 9460 | 77% |
SDS | 全血 | 1% | 存在 | 没有 | 存在 | 2450 | 20% |
CHAPS | 全血 | 1% | 存在 | 没有 | 存在 | 8020 | 66% |
无去垢剂 | 全血 | 0% | 没有 | 明显 | 明显 | 11690 | 96% |
蒸馏水 | 血浆 | 0% | 没有 | 没有 | 没有 | 12240 | 100% |
样品 | 在混合全血后的去垢剂浓度(%) | 溶血 | 磁性颗粒与反应容器壁的黏附 | 磁性颗粒的聚集 | 发光强度 | 回收比例 | |
曲通X-100 | 全血 | 0.01 | 没有 | 有 | 有 | 1084 | 19% |
曲通-100 | 全血 | 0.1 | 没有 | 有 | 有 | 1424 | 25% |
曲通-100 | 全血 | 0.5 | 有 | 没有 | 有 | 4288 | 75% |
曲通-100 | 全血 | 1 | 有 | 没有 | 有 | 2800 | 49% |
曲通-100 | 全血 | 10 | 有 | 没有 | 有 | 1422 | 25% |
吐温20 | 全血 | 0.01 | 没有 | 有 | 有 | 992 | 17% |
吐温20 | 全血 | 0.1 | 没有 | 有 | 有 | 1268 | 22% |
吐温20 | 全血 | 0.5 | 没有 | 有 | 有 | 2696 | 47% |
吐温20 | 全血 | 1 | 没有 | 没有 | 没有 | 3520 | 62% |
吐温20 | 全血 | 10 | 没有 | 没有 | 没有 | 2368 | 42% |
吐温80 | 全血 | 0.01 | 没有 | 有 | 有 | 1304 | 23% |
吐温80 | 全血 | 0.1 | 没有 | 有 | 有 | 1436 | 25% |
吐温80 | 全血 | 0.5 | 没有 | 没有 | 有 | 4256 | 75% |
吐温80 | 全血 | 1 | 没有 | 没有 | 没有 | 4564 | 80% |
吐温80 | 全血 | 10 | 没有 | 没有 | 没有 | 5180 | 91% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 0.01 | 没有 | 有 | 有 | 1596 | 28% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 0.1 | 没有 | 有 | 有 | 2920 | 51% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 0.5 | 没有 | 没有 | 有 | 2720 | 48% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 1 | 没有 | 没有 | 没有 | 4896 | 86% |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 10 | 有 | 没有 | 没有 | 1272 | 22% |
蒸馏水 | 全血 | 0 | 没有 | 有 | 有 | 788 | 14% |
蒸馏水 | 血浆 | 0 | 没有 | 没有 | 没有 | 5696 | 100% |
样品 | 混合全血后的去垢剂浓度(%) | 溶血 | 磁性颗粒与反应容器壁的黏附 | 磁性颗粒的聚集 | 发光强度 | 回收比例 | |
3-(1-吡啶并)-1-丙磺酸盐 | 全血 | 2% | 没有 | 没有 | 有 | 11370 | 86% |
吐温80 | 全血 | 1% | 没有 | 没有 | 没有 | 13460 | 102% |
3-(1-吡啶并)-1-丙磺酸盐+吐温80 | 全血 | 2%1% | 没有 | 没有 | 没有 | 12910 | 98% |
蒸馏水 | 血浆 | 0% | 没有 | 没有 | 没有 | 13180 | 100% |
Claims (16)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP67360/2001 | 2001-03-09 | ||
JP2001067360 | 2001-03-09 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200610093287.2A Division CN1892226B (zh) | 2001-03-09 | 2002-03-07 | 试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1507566A true CN1507566A (zh) | 2004-06-23 |
CN100451651C CN100451651C (zh) | 2009-01-14 |
Family
ID=18925723
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB028095995A Expired - Fee Related CN100451651C (zh) | 2001-03-09 | 2002-03-07 | 测试全血的方法 |
CN200610093287.2A Expired - Fee Related CN1892226B (zh) | 2001-03-09 | 2002-03-07 | 试剂盒 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200610093287.2A Expired - Fee Related CN1892226B (zh) | 2001-03-09 | 2002-03-07 | 试剂盒 |
Country Status (8)
Country | Link |
---|---|
US (1) | US7338809B2 (zh) |
EP (1) | EP1376127B9 (zh) |
JP (1) | JP4167491B2 (zh) |
CN (2) | CN100451651C (zh) |
DE (1) | DE60234821D1 (zh) |
ES (1) | ES2340144T3 (zh) |
HK (1) | HK1066861A1 (zh) |
WO (1) | WO2002073203A1 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101063111B (zh) * | 2006-04-26 | 2010-08-25 | 上海复星医药(集团)股份有限公司 | 一种提高肌酸激酶液体双试剂稳定的方法 |
CN105021805A (zh) * | 2015-08-21 | 2015-11-04 | 三诺生物传感股份有限公司 | 一种人体生理参数检测结果的校正方法 |
CN110108531A (zh) * | 2019-05-14 | 2019-08-09 | 深圳天深医疗器械有限公司 | 一种排除医学检测中血细胞干扰的方法 |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7723099B2 (en) | 2003-09-10 | 2010-05-25 | Abbott Point Of Care Inc. | Immunoassay device with immuno-reference electrode |
WO2007034842A1 (ja) * | 2005-09-20 | 2007-03-29 | Japan Advanced Institute Of Science And Technology | Nmr検出セル、nmr測定方法及びnmr測定用装置 |
WO2007122732A1 (ja) * | 2006-04-24 | 2007-11-01 | Wako Pure Chemical Industries, Ltd. | 被分注液の分注機構、分注装置及び分注方法 |
WO2008053973A1 (en) | 2006-11-02 | 2008-05-08 | Kyowa Medex Co., Ltd. | Method of immunoassay of component to be measured |
KR101904497B1 (ko) * | 2010-01-29 | 2018-10-04 | 가부시키가이샤 엘에스아이 메디엔스 | 인간 sCD14―ST의 분석 방법 |
US20110262989A1 (en) * | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US9091677B2 (en) * | 2010-08-09 | 2015-07-28 | Beckman Coulter, Inc. | Isotonic buffered composition and method that enables counting of cells |
US10191038B2 (en) | 2011-03-28 | 2019-01-29 | Lsi Medience Corporation | Immunological measuring method and measuring kit for whole blood sample |
KR101988600B1 (ko) * | 2011-06-29 | 2019-06-12 | 가부시키가이샤 엘에스아이 메디엔스 | 비특이 반응 억제제, 비특이 반응 억제 방법 및 키트 |
CN102305858B (zh) * | 2011-07-26 | 2013-08-21 | 深圳市国赛生物技术有限公司 | 一种检测降钙素原的试剂盒 |
CN111044718B (zh) * | 2019-12-20 | 2022-05-17 | 北京指真生物科技有限公司 | 一种全血的免疫发光分析用稀释液及其分析方法 |
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US4313927A (en) * | 1979-10-19 | 1982-02-02 | Ames-Yissum Ltd. | Immunoassay method for detecting viral antibodies in whole blood samples |
IL85532A (en) * | 1987-03-13 | 1992-03-29 | Coulter Electronics | Method and lytic reagent system for isolation,identification and/or analysis of leukocytes from whole blood samples |
JP2627191B2 (ja) * | 1989-06-26 | 1997-07-02 | 株式会社新川 | リードフレーム分離装置 |
US5284771A (en) * | 1991-12-05 | 1994-02-08 | Miles Inc. | Reagent compositions and their use in sphering cells |
JPH06167495A (ja) * | 1992-07-14 | 1994-06-14 | S R L:Kk | 凝集イムノアッセイ法 |
JPH06265554A (ja) | 1993-03-11 | 1994-09-22 | Daikin Ind Ltd | 血液生化学成分の分析方法およびその装置 |
EP0722087B1 (en) | 1994-07-29 | 2005-06-29 | Mitsubishi Kagaku Iatron, Inc. | Measurement system using whole blood |
JP3584255B2 (ja) * | 1995-09-06 | 2004-11-04 | アークレイ株式会社 | 全血分析用要素 |
TW373070B (en) * | 1995-09-13 | 1999-11-01 | Kyoto Daiichi Kagaku Kk | Analysis element and method for preparing the same |
JP3780599B2 (ja) * | 1996-02-09 | 2006-05-31 | 日本光電工業株式会社 | 抗原抗体反応物質の測定方法 |
JP3249919B2 (ja) | 1996-07-30 | 2002-01-28 | 株式会社堀場製作所 | 免疫測定方法 |
US5876935A (en) | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
WO1999018438A1 (en) * | 1997-10-02 | 1999-04-15 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
AU1203199A (en) * | 1997-11-18 | 1999-06-07 | Bio-Rad Laboratories, Inc. | Multiplex flow immunoassays with magnetic particles as solid phase |
CN1117283C (zh) * | 1998-04-07 | 2003-08-06 | 厦门大学 | 用于人畜旋毛虫病快速诊断的试剂及其制备方法 |
JP2000065830A (ja) * | 1998-08-21 | 2000-03-03 | Nippon Koden Corp | 抗原抗体反応物質の測定方法および測定装置 |
-
2002
- 2002-03-07 EP EP02703933A patent/EP1376127B9/en not_active Expired - Lifetime
- 2002-03-07 WO PCT/JP2002/002139 patent/WO2002073203A1/ja active Application Filing
- 2002-03-07 CN CNB028095995A patent/CN100451651C/zh not_active Expired - Fee Related
- 2002-03-07 ES ES02703933T patent/ES2340144T3/es not_active Expired - Lifetime
- 2002-03-07 JP JP2002572413A patent/JP4167491B2/ja not_active Expired - Fee Related
- 2002-03-07 CN CN200610093287.2A patent/CN1892226B/zh not_active Expired - Fee Related
- 2002-03-07 DE DE60234821T patent/DE60234821D1/de not_active Expired - Lifetime
-
2003
- 2003-09-09 US US10/657,118 patent/US7338809B2/en not_active Expired - Lifetime
-
2004
- 2004-12-10 HK HK04109808.9A patent/HK1066861A1/xx not_active IP Right Cessation
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101063111B (zh) * | 2006-04-26 | 2010-08-25 | 上海复星医药(集团)股份有限公司 | 一种提高肌酸激酶液体双试剂稳定的方法 |
CN105021805A (zh) * | 2015-08-21 | 2015-11-04 | 三诺生物传感股份有限公司 | 一种人体生理参数检测结果的校正方法 |
CN110108531A (zh) * | 2019-05-14 | 2019-08-09 | 深圳天深医疗器械有限公司 | 一种排除医学检测中血细胞干扰的方法 |
CN110108531B (zh) * | 2019-05-14 | 2022-02-11 | 深圳天深医疗器械有限公司 | 一种排除医学检测中血细胞干扰的方法 |
Also Published As
Publication number | Publication date |
---|---|
EP1376127B9 (en) | 2010-09-29 |
US7338809B2 (en) | 2008-03-04 |
US20040048397A1 (en) | 2004-03-11 |
WO2002073203A1 (fr) | 2002-09-19 |
JP4167491B2 (ja) | 2008-10-15 |
DE60234821D1 (de) | 2010-02-04 |
CN100451651C (zh) | 2009-01-14 |
EP1376127A4 (en) | 2006-08-30 |
CN1892226B (zh) | 2011-08-10 |
EP1376127A1 (en) | 2004-01-02 |
JPWO2002073203A1 (ja) | 2004-07-08 |
CN1892226A (zh) | 2007-01-10 |
ES2340144T3 (es) | 2010-05-31 |
EP1376127B1 (en) | 2009-12-23 |
HK1066861A1 (en) | 2005-04-01 |
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