CN1495273A - 评估结肠直肠癌 - Google Patents
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Abstract
通过分析一组基因的表达对相信患有结肠直肠癌的患者的结肠直肠癌存在与否或其可能状态进行评估的方法。本发明还涉及以多种媒介物，如微阵列作为含有基因表达分布图的试剂盒。
Description
背景
本申请要求2002年3月29日申请的序列号为60/368,798的美国临时申请的利益。
本发明涉及基于生物样本基因表达分布图的结肠直肠癌的诊断和预测。
结肠直肠癌是一种异源疾病，由据认为是通过三种主要分子机制形成的肿瘤组成：1)与染色体不稳定性相结合的多发性结肠腺癌(APC)基因，或β-连环蛋白基因的突变，2)与微卫星序列不稳定性相关的DNA错配修复基因如MLH1，MSH2，PMS2和MSH6的突变，和包含短重复序列基因的突变，以及3)由肿瘤抑制基因启动子区的过甲基化诱导的基因沉默。结肠直肠癌个体的遗传互补可能包括遗传不稳定性、特异突变和基因沉默的不同结合。染色体不稳定性(CIN)一般是癌症的普遍特征。它意味着其中所有的或大部分的染色体丢失或增加了的非整倍体表型。伴随短重复序列突变率的增加，在二倍体肿瘤中发现了微粒体不稳定性(MIN)。这两种遗传不稳定性的形式在结肠直肠癌中很普遍。
因此结肠直肠癌具有复杂的起源并且包括不同生物通路间大量的相互作用。用于协助提供诊断、预测或治疗监测结果的血清标记、组织学和细胞学检查通常不具有所期望的可信度。同时，使用单独的遗传标记(例如一特殊基因的表达增加)可能是有益的，癌症的多样性使遗传标记的组合可能成为最好的途径。
发明概述
本发明是评估结肠直肠癌存在或不存在，或者被认为患有结肠直肠癌的患者其可能状态的方法。在本方法中，通过分析患者样本的基因表达分布图确定患者是否得了结肠直肠癌，患者是否未得结肠直肠癌，患者是否可能要得结肠直肠癌，或正在接受治疗的结肠直肠癌患者对治疗的反应。
本发明的一个方面涉及用于实施该方法的制品。这样的制品包括基因表达分布图或固定于机器可读媒介如计算机可读媒介的基因表达分布图的表现物。
用来鉴别基因表达分布图的制品也可以包括用于捕捉和/或显示基因存在，缺失或表达程度的基片或平面，如微阵列。
发明详述
很少发现组织样本中单纯的特定核苷酸序列的存在或缺失具有诊断或预测价值。另一方面，关于多种蛋白、肽或mRNA表达的信息正越来越受到重视。具有表达蛋白、肽或mRNA(这样的序列是指“基因”)的潜在可能的特定核苷酸序列仅在给定细胞的基因组中存在并不能决定该蛋白、肽或mRNA一定会在给定的细胞中表达。给定的基因能否表达蛋白、肽或mRNA以及表达到何种程度取决于多种复合因子。不考虑理解和评估这些因子的困难程度，检测基因表达可以为如肿瘤发生、转移、细胞凋亡以及其他临床有关现象等重要事件的发生提供有用的信息。通过基因表达分布图可以发现基因激活或失活程度的相对指标。本发明的基因表达分布图可用于结肠直肠癌患者的诊断和治疗。
样品制备需要收集患者样本。本发明使用的病例样本是那些疑为含有患病细胞的样本，如取自结肠样本或外科切除样本的上皮细胞。获得可疑样本的一种有用技术是激光捕捉微切片技术(LCM)。激光捕捉微切片技术提供了一种选择需研究细胞的方法，并最小化由细胞种类的不均一性造成的易变性。因此，正常细胞和癌细胞之间的基因表达中度或细小的变化都可以被轻易检测到。在优选的方法中，样本包括从外周血中提取的循环系统上皮细胞。这可以按照多种方法获得，但最优化的方法是参考引用Immunivest公司的美国专利6,136,182所描述的磁性分离技术。一旦得到含有所关注细胞的样本，就提取其RNA，扩增得，并获得基因表达分布图，优选的通过微阵列获得合适组配的基因。
确证基因表达分布的优选方法包括，确定一个能够编码蛋白质或肽的基因所产生的RNA的量。这可通过逆转录酶PCR(RT-PCR)，竞争性RT-PCR，实时RT-PCR，差异显示RT-PCR，Northern杂交分析和其他相关实验实现。当可能用单个PCR反应实施这些技术时，最好扩增来自mRNA的互补DNA(cDNA)或互补RNA(cRNA)并用微阵列对其分析。对本领域的技术人员来说大量不同的阵列构型及其生产方法是已知的，并且也在如下的美国专利中有描述：5,445,934；5,532,128；5,556,752；5,242,974；5,384,261；5,405,783；5,412,087；5,424,186；5,429,807；5,436,327；5,472,672；5,527,681；5,529,756；5,545,531；5,554,501；5,561,071；5,571,639；5,593,839；5,599,695；5,624,711；5,658,734；和5,700,637；这些专利披露的技术在此引入作为参考。
微阵列技术允许同时检测数千基因的稳定状态的mRNA水平，因此它为检测非控制的细胞增殖效果，如启动、抑制或调节等提供了一个强有力的工具。现在两种微阵列技术正被广泛使用。第一种是cDNA阵列，第二种是寡核苷酸阵列。尽管这些芯片在构造上存在差异，但其下游数据的分析和输出实质上是相同的。这些分析的结果典型地是对由标记探针获得的信号强度的测定，所述的探针用于检测与阵列上已知位点的序列杂交的、来自样品的cDNA序列。典型地，所述的信号强度与cDNA的含量，或者说样本细胞中所表达的mRNA的量成比例。有许多这样的技术可以获得并且是有用的。优选的检测基因表达的方法可以在美国专利中找到，如Linsley等人的专利6,271002；Friend等人的专利6,218,122；Peck等人的专利6,218,114；Wang等人的专利6,004,755，其所公开的内容均在此引入作为参考。
基因表达水平的分析通过比较强度进行。最好是通过制备一种待测样品中基因表达强度相对于对照样品中基因表达强度的比值矩阵进行。例如，可以将疾病组织中基因表达的强度与相同类型正常组织中的表达强度进行比较(例如患病的结肠组织样本对正常的结肠组织样本)。这个表达强度的比值显示出被测样本和对照样本中基因表达的倍数变化。
基因表达分布图也可以通过很多方法显示。最普遍的方法是用树突状图处理原始的荧光强度或矩阵系数，其中列表示被测样品，行表示基因。数据经这样的处理后那些具有相似表达分布的基因就会相互邻近。每个基因的表达比率可用一种颜色显示。例如，小于一的比率(表示下调)可以出现在图谱的蓝色部分，而大于一的比率(表示上调)可以出现在图谱的红色部分。商业上可购买到的计算机软件程序很适合显示这样的数据，包括Silicon Genetics有限公司的“GENESPRING”，Partek有限公司的“DISCOVERY”和“INFER”软件。
用于本发明的方法的受调节基因如表1所示。患病细胞中差异表达的基因表现为上调或下调。上调和下调是相对的术语，表示可检测到的相对于某种基线的基因表达的量的差异(超出检测系统的噪音影响)。在这种情况中，基线是正常细胞测得的基因表达。再使用同样的测量方法确定患病细胞中所关注的基因相对于基线水平是上调或下调。上下文中，患病的，是指伴随细胞非控制增殖而出现的机体状态的改变，此种改变中断。扰乱，或潜在的打乱机体功能的适当表现。当某人基因型或表型的一些方面一致表现出存在特定疾病时，他就被诊断为患有该种疾病。作出诊断或预测的行为包括确定疾病表象/状态的情况，如治疗监测。在治疗监测中，比较经过一段时间治疗后的基因表达确定基因表达分布图是否有变化，或其变化与正常组织的模式更为一致来考虑疗程的效果从而作出临床判定。
优选的，上升或下降调节的水平是以杂交的微阵列探针测量强度的成倍变化来区别的。2倍差异或p值小于0.5对于作出这样的辨别是优选的。即当认为与在正常细胞中相比一个基因在患病细胞中差异表达时，患病细胞中的强度至少是超出正常细胞的2倍或低于正常细胞2倍。差异倍数越大，基因越优选用作诊断。本发明选择用作分析表达分布图的基因具有产生使该基因区别于正常基因或非调节基因的信号的表达水平，该信号量超出使用临床实验室检测设备的背景量。
利用统计值可用于确定地使调节基因与非调节基因和噪音区别开。通过统计检验发现在不同样本组之间有显著差异的基因。Student’s T检验就是一个发现两组之间显著差异的有力的统计检验的例子。p值越小，不同组之间基因显示差异的证据就越有说服力。然而既然微阵列可以同时检测多个基因，那么就需要同时进行万次的统计检验。因此，就存在偶尔看到极小p值的可能，并利用Sidak校正以及作出随机化/排列实验进行调整。T检验中p值小于.05就证明基因是差异显著的。更有说服力的证据是加入Sidak校正因子后p值小于.05。对于每组中的大量样品，经过随机化/排列检验后p值小于.05是差异显著的最有说服力的证据。
另一个可用来选择产生强于非调节基因和噪音信号的基因的参数是绝对信号差异测量值。优选的，调节基因的表达形成的信号与正常基因或非调节基因(以绝对值为基础)至少有20％的差异。更优选的，调节基因形成的表达模式与正常基因或非调节基因至少有30％的差异。
将基因分组以便于通过获得一组基因中一套基因的信息为诊断、预测或治疗等相关临床判断提供可靠的基础。这些套基因构成了本发明的框架(portfolios)。此例中，由该框架支持的判断包括结肠直肠癌。基因表达分布图的框架包含实施例3中描述的基因联合组合。对于大多数诊断标记物来说，最好使用尽量少的标记物就足以充分作出一个正确的医学判断。这就避免了使用那些悬而未决需作进一步分析的治疗措施带来的延误，同时又避免了时间和资源的不合理使用。此例中，这样的最小量的框架包含实施例4中的基因组合。
优选的，构建这样的框架以保证该框架中的基因组合相对于单个基因或随机选择的基因组合显示出更好的灵敏度和特异性。在本发明中，框架的灵敏度反映在一个基因在疾病状态下相对于其在正常状态下所表现出的基因表达的成倍差异中。特异性可以反映在对所关注的基因表达信号相关性的统计测量中。例如，标准差就是这样一个有用的度量。当考虑将一组基因掺入到某一框架中时，表达测量值的标准差越小其特异性越大。其他检测变量，如相关系数也包括在使用范围内。
最优选地，建立基因表达框架的方法是运用优化算法，如在确定股票投资中广泛使用的平均方差算法的。这一方法在同日期的Tim Jatkoe等人标题为“笼统投资选择”的同时待审的专利申请中有详细表述。该方法实质上要求一套输入值(财务应用中的库存，这里指通过强度测定的表达)，其可优化收到的返回值(例如形成的信号)以进行应用，同时最小化返回值的可变性。许多商业软件程序适合进行这样的操作。在整个说明书中称为“Wagner软件”的“Wagner联合均值-方差优化应用程序”是优选的。这个软件使用“Wagner联合均值-方差优化库”中的功能，来确定有效边界和Markowitz意义上优选的优化框架。
由于该软件用于金融分析目的，因此使用这种类型的软件需要将微阵列数据进行转换，使数据以股票回报值和风险测量值的方式输入处理。例如，当Wagner软件与微阵列强度测定协同应用时，要运用以下的数据转换方法。
首先通过确定那些其表达至少显示出某种微小差异水平的基因，对基因进行预选择。优选的预选择过程如下所述。选择一个基线组。典型的基线组包含来自未患有所述疾病的群体的基因。例如，在选择所用于诊断乳腺癌的框架时，可以将未患乳腺癌患者的样本用作制备基线组。一旦选择了基线组，就可以计算出基线组样本中每个基因的表达指标的算术平均值和标准差。典型的，这个指标是微阵列记录的荧光强度。随后将通过计算得到的统计学数据用于计算每个基因的基线值(X*标准差+均值)。这就是基因的基线记录，其它所有样本都要与其相比较。X是由制定框架的人选择出来的严格变量。X的数值越大就越严格。优选的X数值范围是0.5-3，更优选的是2-3，最优选的是3。
随后计算每个实验样本(那些所关注的样本)与基线记录的比值。再将这些比值转换成易于软件处理的底数为10的对数值。该方法使下调基因显示为使用Wagner软件并按照Markman均值-方差算法所必需优化的负值。
将包含这些转换比值的预处理数据作为输入替代用于金融分析目的的Wagner软件中通常使用的资产回报值。
一旦制定出有效边界，对于一个给定的输入水平值(返回值)或对应于边界上的一个点的方差，就可以选择出一个优化框架。这些输入值或方差是制定框架的人预先确定的标准数集。换句话说，寻求最适框架的人决定一个可接受的输入水平(表明灵敏度)或者一个给定的方差水平(表明特异性)，选择位于该输入水平或方差相应的有效边界选择基因。当选定了输入水平或方差，Wagner软件就能够选择出这样的基因。Wagner软件可以像对股票笼统投资中每支股票所做的那样，分配所述框架中每个基因的分量。
将患者样本框架中的基因中的表达，与用于构建所述框架的差异表达基因的计算值相比较，就可以确定该样本是否患有该框架所诊断的疾病。优选的，首先通过将在选择框架的过程中给每个基因分配的权重乘以框架中每个基因的强度值，加和得到框架值。然后用(Y*标准差+基线组中框架的均值)计算临界值，其中Y是一个严格数值，具有与上述X相同的含义。一个样本具有的框架值大于基线组的框架值，则此样本就被归为患病类。需要时，可以根据已知的统计学方法重复上述过程提高可信度水平。
任选的，可以重复进行上述过程直到获得预期的最高准确度。
框架选择的过程和未知量的表征过程概括如下：
1.选择基线组
2.计算基线组样本中每个基因的均值和标准差
3.计算每个基因的(X*标准差+均值)。这就是基线记录，其它所有的样本都要与这个基线记录相比较。X是一严格变量，X数值越大就越严格。
4.计算根据步骤3得到的每个实验样本对基线记录的比值。
5.转换数值，使值小于1的比值成为负数(例如使用底数为10的对数)。(现在下调的基因有正确的负值以符合MV优化的需要)。
6.将这些转换的比值作为输入值，替换软件应用中通常使用的资产回报值。
7.该软件将标绘出有效的边界线，并且返回一组沿此有效边界线上任意一点的优化框架。
8.在此有效边界线上选择一个所需的返回值或者方差
9.通过将在选择框架的算法中所给定的权重乘以每个基因的强度值，加和计算每个样本的框架值。
10.将基线组的框架值的均值加上Y与基线组的框架值的标准差的乘积，计算出临界值。大于该临界值的数值应被归为实验组。
11.任选的，可以重复进行上述过程，直到获得预期的最佳准确度。
可选择的，首先通过确定那些其表达至少显示出某种微小差异水平的基因，对基因进行预选择。在这个可选择的方法中，预选择优选的以
给出的阈值为基础，其中μt是已知患病或有该疾病的样本子集的均值，μn是正常样本子集的均值，σt+σn表示联合标准差。通过对根据
得到的数据的预选择，噪音中止点的信号也能在其中使用。这就确保基于差异调节进行预选择的基因具有临床上的显著差异。即超过了适于测定各种临床参数的仪器产生的噪音水平。对于根据这些标准预选的每个标记物建立一个矩阵，其中列表示样本，行表示标记物，并且每个元件都是根据
对该标记物的表达进行标准化后所得到的强度测量值，其中I代表强度测量值。
也可以通过设置附加的边界条件来定义优化框架。例如，框架的大小可限定为固定的值域或标记物的数目。这或者可以通过制定更为严格的预选标准(例如以
代替
)或通过使用程序特征如限制组合大小来实现。例如可以设置边界条件使有效边界线从最优化的10个基因中选出。也可以使用所有的预选基因确定有效边界线后再限定选择基因的数目(例如不超过10个)。
选择框架的过程也包括应用探索式的规则。优选的，这样的规则是基于生物学和对用于产生临床结果的技术理解的基础上制定的。更优选的是，它们应用到优选方法的输出数据中。例如，选择框架的均方差法可以应用于乳腺癌样本中大量差异表达基因的微阵列数据。这种方法得到的输出数据将会是一套优选的基因，包括既在外周血中表达也在患病乳腺组织中表达的一些基因。如果这种检测方法用到的样本来自外周血，并且某些基因既在乳腺癌个例也在外周血中差异表达，那么就可以应用探索式的规则，从排除了那些在外周血中差异表达的基因而得到的有效边界线上选择框架。当然，这个规则可以在形成有效边界线前应用，例如，将该规则应用到数据的预先选择中。
可以应用其他与未有定论的生物学问题无必然联系的探索式规则。例如，可以应用只有给定百分比的框架可用特定的基因表示的规则。商业上可购买的软件如Wagner软件就提供了这类型的探索式规则。这是非常有用的，例如，除了正确度和精密度(例如，预支专利转让费)等，其他因素也会影响包括一个或多个基因的可用性。
本发明的一个方法包括如上所述将基因表达分布图与多种基因(或分布图)比较以进行诊断。每个基因的基因表达分布图包括固定于如计算机可读的媒介物上的框架。这可以采取很多形式。例如，可以建立一个输入表示疾病的信号范围(例如强度测定值)的表格。可以将病例的实际数据与表格中的值进行比较以确定该患者的样品属于是正常的或患病的。在一个改进的具体实例中，表达信号的模式(例如荧光强度)以数字或图形方式记录。再将与病例样本相结合使用的框架中的基因表达模式与上述表达模式比较。然后利用模式比较软件确定该病例样本是否具有表示所讨论的疾病的模式。当然这些比较也可用于确定该病例的结果是否正常。再将样本的表达分布图与正常或对照细胞的框架相比较。如果样本表达模式与结肠直肠癌的表达模式一致，那么(无抵消性的医学理由)该病例就被诊断为结肠直肠癌阳性。如果样本表达模式与正常或对照细胞的表达模式一致，那么该病例就被诊断为结肠直肠癌阴性。
许多公知的识别模式的方法是适用的。下列参考提供了一些例子：
加权阈值Weighted Voting：
Golub，TR.，Sclonim，DK.，Tamaya，Huard，C.，Gaasenbeek，M.，Mesirov，JP，Coller，H.，Loh，L.，Downing，JR.，Caligiuri，MA.，Bloomfield.，CD.，Lander，ES.癌症的分子分类：利用基因表达检测的分类发现和分类预测。科学Science 286：531-537，1999
支持矢量机器Support Vector Machine：
Su，Al.，Welsh，JB.，Sapinoso，LM.，Kern，SG.，Dimitrov，P.，Lapp，H.，Schultz，G.，Powell，SM.，Moskaluk，CA.，Frierson，HF.Jr，Hampton，GM.利用基因表达信号的人类癌症的分子分类。癌症研究Cancer Research 61：7388-93，2001
Ramaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，GH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T.，Gerald，W，Loda，M.，Lander，ES.，Gould，TR利用肿瘤基因表达信号的多种类癌症诊断。美国国家科学院院刊Proceedingsof the National Academy of Sciences of the USA 98：15149-15154，2001
K-最邻近值K-nearest Neighbors：
Ramaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，GH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T.，Gerald，W.，Loda，M.，Lander，ES.，Gould，TR.利用肿瘤基因表达信号的多种类癌症诊断。美国国家科学院院刊Proceedingsof the National Academy of Sciences of the USA 98：15149-15154，2001
相关系数Correlation Coefficients：
van’t Veer LJ，Dai H，van de Vijver MJ，He YD，Hart AA，Mao M，Peterse HL，vander Kooy K，Marton MJ，Witteveen AT，Schreiber GJ，Kerkhoven RM，Roberts C，LinsleyPS，Bemards R，Friend SH.基因表达分布图预测乳腺癌的临床结果。自然Nature.2002 Jan 31：415(6871)：530-6
本发明的基因表达分布图也可以与其他在癌症诊断、预测、或治疗监测中所使用的非遗传的诊断方法联合使用。例如，在一些情况中，将如上所述的基于基因表达能力的诊断方法与从如血清蛋白标记(例如癌胚性抗原)的传统标记物中得到的数据结合使用是很有益的。这样存在的标记物范围包括如CA19-9，CA125，CK-BB和鸟苷酸环化酶C的分析物。在这样的一个方法中，对治疗患者周期性采血，然后将血样作上述血清标记之一的酶联免疫检测。当标记的浓度暗示了肿瘤复发或治疗失败时，可以随后对样本源作基因表达分析。如果存在所怀疑的肿块，就进行更好的针吸采样并如上所述对取自肿块的细胞进行基因表达分布图分析。可选择的，组织样本可以取自刚摘取肿瘤的组织的邻近区域。当其他检测结果不确定时，这种方式特别有用。
当其它诊断的可靠性值得怀疑时，联合使用遗传标记和其他诊断是最优选的。例如，众所周知CEA血清水平在相当程度上受到与患者癌症状态毫无关系的因子的影响。当一个患者被监测到在接受结肠癌治疗后显示常规CEA检测高水平时，那联合进行基因表达/CEA检测是有益的。
本发明的制品包括用于治疗、诊断、预测和其它疾病评估的基因表达分布图的表示物。这些分布图表示物简化为可被机器自动可读的媒介物，如计算机可读媒介(磁性的，光学的及类似的)。制品也包括在这种媒介中评估基因表达分布图的指令说明。例如，制品可能包括具有用于比较上述基因组合的基因表达分布图的计算机指令的CD ROM。制品可能也含有基因表达分布图的数字记录以用于与病例样本的基因表达数据进行比较。可选择的，所述的分布图可以不同的表现格式记录。图形记录就是这样的一种格式。上文提到的“GENESPRING”和“DISCOVER”计算机程序中整合的聚类算法就可很好的协助这些数据形象化。
根据本发明生产制造的不同类型的物品是指用于揭示基因表达分布图的媒介物或格式化检测。这些可以包括，例如微阵列，其中将序列互补物或探针固定于基片上，它与表示所关注基因的序列联合建立一个易读行列式。可选择的，根据本发明的制品可以被制作成进行杂交、扩增，以及形成表示所关注的基因表达水平的信号以用来检测结肠直肠癌的试剂盒。
根据本发明制备的试剂盒包括确定基因表达分布图的格式化检测。这就包括了进行检测所需的如试剂和说明指令等所有或部分材料。
本发明进一步用下列非限定性的实施例说明。
实施例：根据本发明的基因分析是通过参考GenBank数据库中的基因ID号鉴定的。它们与编码产生蛋白质或肽的全长核苷酸序列典型相关。本领域的技术人员公认全长核苷酸序列的鉴定不是一个分析观点所必需的。即可以根据公知的为评估相关基因表达设计探针的原理进行部分序列或ESTs选择。
实施例1-样品处理和LCM
收集接受结肠直肠肿瘤外科手术患者的27个新鲜冷冻组织样本。19个样本是结肠直肠恶性肿瘤实例，8个样本是正常的结肠粘膜。组织在获得后的20-30分钟内快速液氮冷冻，并随后贮存于-80℃。为了激光捕捉，将样本切片(6μm)，一切片封固于玻璃载片上，另一切片封固于已被固定于玻璃载片(Micro SlidesColorfrost，VWR Scientific，Media，PA)上的膜(P.A.L.M.)上.封固于玻璃载片上的切片在冷的丙酮中固定，并用Mayer’s苏木精(Sigma，St.Louis，MO)染色.病理学家为诊断和定级分析样本。由附带的使用Dukes分级的外科病理学和临床报告进行临床阶段评估。封固于膜上的切片在100％乙醇中固定5分钟，在曙红/100％乙醇(100μg曙红溶于100ml脱水乙醇)中复染1分钟，快速在100％乙醇中浸泡一次以除去未结合染料，空气中干燥10分钟。
结肠直肠腺癌中的两个为等级1，10个为等级2，5个为等级3。一个恶性肿瘤样本是盲肠的类癌瘤，另一个是转移的黑色素瘤的损伤。两个腺癌样本表现为粘蛋白状亚型，一个为章形细胞亚型。腺癌样本按Dukes分级划分如下：Dukes A：2，Dukes B：5，Dukes C：7，Dukes D：3。六个腺癌样本在手术前放疗过。
在LCM中使用前，膜(LPC-MEMBERANE PEN FOIL 1.35μmNo.8100，P.A.L.M.GrmbH Mikrolaser Technologie，Bemried，Germany)和载片经过预处理以除去RNA酶，并且增强组织样品在膜上的吸附。简要的，载片用DEPH2O洗涤，膜用RNase AWAY(Molecular Bioproducts，Inc.，San Diego，CA)洗涤并用DEP H2O冲洗。膜粘附到玻璃载片上后，载片于120℃烘烤8小时，用TI-SAD处理(Diagnostic Products Corporation，Los Angeles，CA，以1∶50溶于DEPH2O，用脱脂棉过滤)，+37℃孵育30分钟。使用前，立即将10μl小份的RNA酶抑制剂溶液(RNA酶蛋白质抑制剂2500U＝33U/μl N211A，Promega GmbH，Mannheim，Germany，0.5μl溶于400μl冷的含有0.15mol NaCl，10mmol TrispH8.0，0.25mmol二硫苏糖醇的溶液)铺展到膜上封固组织样本的位置。
封固于膜上的组织切片用于LCM。利用PALM自动微光束技术(P.A.L.M.Microlaser Technologie，Carl Zeiss，Inc.，Thornwood，NY)可捕捉的约2000个上皮细胞样品，并连接到Zeiss Axiovert 135显微镜中(Carl Zeiss Jena GmbH，Jena，Germany)。正常粘膜的外周基质和癌症样本中偶然的干扰基质成分都包括在内。捕捉到的细胞置于100％乙醇的小管中于-80℃保存。
实施例2-RNA提取和扩增
利用Zymo-Spin柱(Zymo Research，Orange，CA92867)提取通过LCM捕捉的细胞的总RNA。将约2ng总RNA重悬于10μl水并且利用T7 RNA聚合酶进行两轮扩增直至扩增的RNA产量约为50μg。
实施例3-cDNA微阵列杂交和定量
利用一套由约20,000个人cDNA克隆组成的cDNA微阵列检测样本。将约30个植物基因印迹到微阵列上作为非特异杂交对照。利用LCM捕捉细胞的5μg aRNA合成Cy3标记的cDNA探针。探针用Qiagen’s核苷酸去除柱(Nucleotide Removal Columns)纯化然后经14-16小时杂交到微阵列上。载片在扫描前经过洗涤并于空中干燥。扫描微阵列的Cy3荧光并用ImaGene软件(Biodiscovery，Los Angeles，CA)定量。对于每个cDNA克隆，进行重复点和重复阵列的四次测量并将所得强度值平均。
利用第三代微阵列点样仪(Molecular Dynamics)将cDNAs印迹到氨基硅烷涂层的载片上(Corning)。cDNAs经PCR扩增，纯化(Qiagen PCR纯化试剂盒)，并与10M NaSCN印迹缓冲液1∶1混合。杂交前微阵列在异丙醇中室温孵育10分钟。探针于95℃孵育2分钟，室温孵育5分钟，然后加到三个平行测定的载片上。用DPX(Fluka)将盖片密封到载片上并于42℃孵育过夜。然后将载片在1×SSC/0.2％SDS和0.1×SSC/0.2％SDS中于55℃洗涤5分钟，0.1×SSC中浸润并干燥后用第三代微阵列扫描仪(Molecular Dynamics)扫描。用AUTOGENE软件(Biodiscovery，Los Angeles)分析每个点的荧光强度。
对芯片强度进行线性标准化使得每个芯片的强度记录75th个百分点相当于值100。芯片上的每个基因通过将该基因的强度记录除以所有样本的基因表达值记录的中间值标准化。在聚类前，那些至少在一个样本中不具有100或更大的强度记录的基因被滤掉了以限制对相似性量度的背景影响。选择一套6255个基因作聚类分析。利用作为相似性测量的相关度进行分级聚类，使具有表现正变化同时不考虑其负变化(Silicon Genetic，Sunnyville，CA)的基因的样品聚类到一起。系统图的每一个主要节点可被认为是样本的一个亚组。通过将每个肿瘤亚组与正常组进行比较鉴定差异表达的基因。筛选是基于
给出的信噪比测量阈值，其中μt是肿瘤子集的平均数，μn是正常样本子集的平均数，σt+σn表示结合标准差。组内一个基因的强度记录的变异系数必须低于0.33，因为基因处于成对方式比较之中。肿瘤组的中间值与正常组的中间值相比大于2或等于2是为上调，小于或等于0.5倍时为下调。如果一个基因符合所有标准则被选择出来。在所有的比较中均被筛选出来的基因即被认为是在结肠直肠癌中稳定调节异常的。利用T检验假定不等方差计算统计学显著性p值。用于聚类的一套基因也用软件包(Partek，St Louis，MO)进行主成分分析(PCA)。然后将数据投射到简化的三维空间。正常的和结肠直肠肿瘤的样本以映射表达水平表现出来。
建立有很大上调差异的基因列表以用来区别肿瘤和正常样本。利用 作为信噪比中止点先挑选出123个基因。以等于或大于1.5的比率作为上调的最低标准。也包括
的基因。已经建立了一个四基因的框架，每个基因在肿瘤和正常细胞间都有至少3倍的差异。
结肠直肠癌中差异表达的基因。与正常结肠粘膜相比，39个基因在所有的肿瘤样本中差异表达。除了一个异常值，37个基因在所有的肿瘤中显著下调。2个上调。基因的同一性通过微阵列上cDNA克隆的测序验证。结果如表1所示。
表1调节基因
| 登记号 | 基因描述 | 平均信号强度(正常) | 平均信号强度(肿瘤) | P值 | |
| AF071569 | 钙调蛋白激酶II基因亚型delta2 | 93 | 39 | 4.64E-09 | Seq.ID No.1 |
| AB014530 | KIAA0630蛋白质的人类mRNA | 108 | 50 | 4.83E-07 | Seq.ID No.2 |
| AK000319 | 人KIAA0630 cDNA | 236 | 69 | 7.84E-06 | Seq.ID No.3 |
| U81504 | AP-3复合物的Beta-3A-衔接蛋白亚基mRNA | 241 | 75 | 3.52E-05 | Seq.ID No.4 |
| AB011166 | 人KIAA0594 cDNA | 116 | 55 | 3.53E-05 | Seq.ID No.5 |
| AB040914 | 人KIAA1481 cDNA | 187 | 59 | 8.85E-05 | Seq.ID No.6 |
| AK025205 | 人FLJ21552 cDNA | 322 | 97 | 0.00013 | Seq.ID No.7 |
| AJ278219 | 脂肪酸羟化酶 | 143 | 53 | 0.00011 | Seq.ID No.8 |
| AB046854 | 人KIAA1634 cDNA | 142 | 59 | 0.00020 | Seq.ID No.9 |
| R00585 | 未知 | 149 | 57 | 1.28E-09 | Seq.ID No.10 |
| S45844 | Spi-B转录因子 | 140 | 43 | 0.00043 | Seq.ID No.11 |
| X98311 | 癌胚性抗原家族成员2(CGM2) | 6137 | 223 | 0.00044 | Seq.ID No.12 |
| BAA78050 | NADPH氧化还原酶同源物 | 163 | 84 | 0.00048 | Seq.ID No.40 |
| N72128 | 未知 | 164 | 77 | 0.00068 | Seq.ID No.13 |
| AB040955 | 人KIAA1552 cDNA | 334 | 120 | 0.00067 | Seq.ID No.14 |
| AF125101 | HSPC040蛋白质 | 363 | 115 | 0.0011 | Seq.ID No.15 |
| AB023229 | 人KIAA1012 cDA | 263 | 88 | 0.00099 | Seq.ID No.16 |
| N95761 | a-L-岩藻糖苷酶基因 | 429 | 104 | 0.00047 | Seq.ID No.17 |
| AK025033 | 人FLJ21380 cDNA | 180 | 85 | 0.0010 | Seq.ID No.18 |
| L10844 | 人细胞生长调节蛋白 | 206 | 101 | 0.0013 | Seq.ID No.19 |
| H96534 | Gp25L2蛋白质的人类mRNA | 147 | 58 | 0.0015 | Seq.ID No.20 |
| AK001521 | 人FLJ10659 cDNA | 157 | 60 | 0.0019 | Seq.ID No.21 |
| AF151039 | HSPC205蛋白质 | 117 | 60 | 0.0017 | Seq.ID No.22 |
| AF052059 | SEL 1L蛋白质 | 168 | 53 | 0.0016 | Seq.ID No.23 |
| N24597 | 未知 | 166 | 62 | 0.0016 | Seq.ID No.24 |
| AK001950 | 内着丝粒蛋白 | 148 | 64 | 0.0029 | Seq.ID No.25 |
| BAA02649 | 巨噬细胞消除受体I型 | 118 | 44 | 0.0031 | Seq.ID No.41 |
| N75004 | 未知 | 98 | 48 | 0.0031 | Seq.ID No.26 |
| W16916 | 人KIAA0260 cDNA | 162 | 61 | 0.0037 | Seq.ID No.27 |
| X52001 | 人类内皮素3mRNA | 89 | 33 | 0.0042 | Seq.ID No.28 |
| T50788 | 未知 | 364 | 102 | 0.0059 | Seq.ID No.38 |
| AJ005866 | 推定的sqv-7样蛋白 | 381 | 163 | 0.0049 | Seq.ID No.29 |
| AF113535 | MAID蛋白质 | 218 | 100 | 0.0053 | Seq.ID No.39 |
| AB037789 | 人KIAA1368 cDNA | 164 | 62 | 0.0068 | Seq.ID No.30 |
| M33987 | 碳酸酐酶 | 652 | 46 | 0.0074 | Seq.ID No.31 |
| M77830 | 桥粒斑蛋白1(DP1) | 184 | 81 | 0.0092 | Seq.ID No.32 |
| H81220 | EST功能域转录因子ELF1 | 113 | 55 | 0.017 | Seq.ID No.33 |
| AF000592 | 人染色体21q11-q21基因组克隆 | 33 | 69 | 1.16E-05 | Seq.ID No.35 |
| AK021701 | 人FLJ11639 cDNA | 31 | 63 | 0.00070 | Seq.ID No.36 |
实施例4结肠直肠肿瘤的优化框架
利用方差优化算法生成多重基因基础的标记，其中包括可结合使用以区别正常和肿瘤样本的基因。利用样本和如实施例1-3所描述的微阵列进行强度检测。肿瘤和正常样本间有5倍差异的数据先被预选出来用作分析。根据这个标准预选出来的基因的表达数据随后作进一步使用。每个基因的强度测量值的平均数和标准差是以非转移性样本作为基线计算出来的。然后对于每个基线基因(X值指定为3)计算差别值X*(标准差+平均值)。这个值用来确保结果框架是有说服力的。再用差别值与基线值的比率计算每个转移性样本。这个比率随后被转换成常用对数。然后这个数据被输入Wanger软件，以产生用来选择4基因框架的有效边界。这套基因包括一未知序列，前胶原I型，核糖体蛋白的大亚基L21，和纤连蛋白。这些基因确定为Seq.ID.No.42，Seq.ID.No.43，Seq.ID.No.44和Seq.ID.No.45的序列。换句话说，形成框架的基因组合可以为关于结肠直肠癌的临床判定提供有用的诊断信息。当使用选自与附加标记(遗传或非遗传的)相关的框架的基因组合特别有益。
优化的基因框架：
＞gi|1264443|gb|N92134.1|N92134 za23f09.r1 Soares fetal liver spleenINFLS Homo sapiens cDNA clone IMAGE：293417 5’similar to gb|M87908|HUMALNE32 Human carcinoma cell-derived Alu RNA transcript，(rRNA)；gbX57025_mal INSULIN-LIKE GROWTH FACTOR IAPRECURSOR(HUMAN)
＞gi|2221047|gb|AA490172.1|AA490172 ab06b08.s1 Stratagene fetalretina 937202 Homo sapiens cDNA clone IMAGE：839991 3’similar to gb：J03464PROCOLLAGEN ALPHA 2(I)CHAINPRECURSOR(HUMAN)
＞gi|2188912|gb|AA464034.1|AA464034 zx86b09.r1 Soares ovarytumor NbHOT Homo sapiens cDNA clone IMAGE：810617 5’similar toSW：RL21_HUMAN P46778 60S RIOSOMAL PROTEINL21
＞gi|834491|gb|R62612.1|R62612 yi12d01.s1 Soares placenta Nb2HPHomo sapiens cDNA clone IMAGE：139009 3’similar to gb：X02761-cds1FIBRONECTIN PRECURSOR(HUMAN)；
使用不同的标准但相同的方法，通过软件选出了又一个四基因框架。这些是Seq.ID.No.42，Seq.ID.No.43，Seq.ID.No.44和Seq.ID.No.45的序列。两个基因与第一个四基因框架重叠。两个优化框架也可以结合形成一个六基因框架。
优化的基因框架：
＞gi|2114953|gb|AA431245.1|AA431245.1 zw78d06.r1Soares_testi_NHT Homo sapiens cDNA cloneIMAGE：782315 5’similar towp：F36H1.2 CE05814 ANKYRIN LIKE
＞gi|2156172|gb|AA443497.1|AA443497 zw34d03.r1 Soares ovarytumor NbHOT Homo sapiens cDNA clone IMAGE：771173
＞gi|2221047|gb|2| AA490172 ab06b08.s1 Stratagen fetal retina 937202Homo sapiens cDNA clone IMAGE：839991 3’similar to gb：J03464PROCOLLAGEN ALPHA 2(I)CHAINPRECUSOR(HUMAN)
＞gi|1264443|gb|N92134.1|N92134 za23f09.r1 Soares fetal liver spleenINFLS Homo sapiens cDNA clone IMAGE：293417 5’similar to gb|M87908|HUMALNE32 Human carcinoma cell-derived Alu RNA transcript，(rRNA)；gbX57025_mal INSULIN-LILE GROWTH FACTOR IA PRECURSOR(HUMAN)；
序列表
<110>WANG，YIXIN
<120>评估结肠直肠癌
<130>CDS 267 US NP
<140>TBD
<141>2003-03-21
<150>60/368,798
<151>2002-03-29
<160>49
<170>PatentIn version 3.1
<210>1
<211>1500
<212>DNA
<213>人
<400>1
atggcttcga ccaccacctg caccaggttc acggacgagt atcagctttt cgaggagctt 60
ggaaaggggg cattctcagt ggtgagaaga tgtatgaaaa ttcctactgg acaaggatat 120
gctgccaaaa ttatcaacac caaaaagctt tctgctaggg atcatcagaa actagaaaga 180
gaagctagaa tctgccgtct tttgaagcac cctaatattg tgcgacttca tgatagcata 240
tcagaagagg gctttcacta cttggtgttt gatttagtta ctggaggtga actgtttgaa 300
gacatagtgg caagagaata ctacagtgaa gctgatgcca gtcattgtat acagcagatt 360
ctagaaagtg ttaatcattg tcacctaaat ggcatagttc acagggacct gaagcctgag 420
aatttgcttt tagctagcaa atccaaggga gcagctgtga aattggcaga ctttggctta 480
gccatagaag ttcaagggga ccagcaggcg tggtttggtt ttgctggcac acctggatat 540
ctttctccag aagttttacg taaagatcct tatggaaagc cagtggatat gtgggcatgt 600
ggtgtcattc tctatattct acttgtgggg tatccaccct tctgggatga agaccaacac 660
agactctatc agcagatcaa ggctggagct tatgattttc catcaccaga atgggacacg 720
gtgactcctg aagccaaaga cctcatcaat aaaatgctta ctatcaaccc tgccaaacgc 780
atcacagcct cagaggcact gaagcaccca tggatctgtc aacgttctac tgttgcttcc 840
atgatgcaca gacaggagac tgtagactgc ttgaagaaat ttaatgctag aagaaaacta 900
aagggtgcca tcttgacaac tatgctggct acaaggaatt tctcagcagc caagagtttg 960
ttgaagaaac cagatggagt aaaggagtca actgagagtt caaatacaac aattgaggat 1020
gaagatgtga aagcacgaaa gcaagagatt atcaaagtca ctgaacaact gatcgaagct 1080
atcaacaatg gggactttga agcctacaca aaaatctgtg acccaggcct tactgctttt 1140
gaacctgaag ctttgggtaa tttagtggaa gggatggatt ttcaccgatt ctactttgaa 1200
aatgctttgt ccaaaagcaa taaaccaatc cacactatta ttctaaaccc tcatgtacat 1260
ctggtagggg atgatgccgc ctgcatagca tatattaggc tcacacagta catggatggc 1320
agtggaatgc caaagacaat gcagtcagaa gagactcgtg tgtggcaccg ccgggatgga 1380
aagtggcaga atgttcattt tcatcgctcg gggtcaccaa cagtacccat caagccaccc 1440
tgtattccaa atgggaaaga aaacttctca ggaggcacct ctttgtggca aaacatctga 1500
<210>2
<211>5761
<212>DNA
<213>人
<400>2
cacaccgcag tatgcggtgc cctttactct gagctgcgca gccggccggc cggcgctggt 60
tgaacagact gccgctgtac tggcgtggcc tggagggact cagcaaattc tcctgccttc 120
aacttggcaa cagttgcctg gggtagctct acacaactct gtccagccca cagcaatgat 180
tccagaggcc atggggagtg gacagcagct agctgactgg aggaatgccc actctcatgg 240
caaccagtac agcactatca tgcagcagcc atccttgctg actaaccatg tgacattggc 300
cactgctcag cctctgaatg ttggtgttgc ccatgttgtc agacaacaac aatccagttc 360
cctcccttcg aagaagaata agcagtcagc tccagtctct tccaagtcct ctctagatgt 420
tctgccttcc caagtctatt ctctggttgg gagcagtccc ctccgcacca catcttctta 480
taattccttg gtccctgtcc aagatcagca tcagcccatc atcattccag atactcccag 540
ccctcctgtg agtgtcatca ctatccgaag tgacactgat gaggaagagg acaacaaata 600
caagcccagt agctctggac tgaagccaag gtctaatgtc atcagttatg tcactgtcaa 660
tgattctcca gactctgact cttctttgag cagcccttat tccactgata ccctgagtgc 720
tctccgaggc aatagtggat ccgttttgga ggggcctggc agagttgtgg cagatggcac 780
tggcacccgc actatcattg tgcctccact gaaaactcag cttggtgact gcactgtagc 840
aacccaggcc tcaggtctcc tgagcaataa gactaagcca gtcgcttcag tgagtgggca 900
gtcatctgga tgctgtatca cccccacagg gtatcgagct caacgcgggg ggaccagtgc 960
agcacaacca ctcaatctta gccagaacca gcagtcatcg gcggctccaa cctcacagga 1020
gagaagcagc aacccagccc cccgcaggca gcaggcgttt gtggcccctc tctcccaagc 1080
cccctacacc ttccagcatg gcagcccgct acactcgaca gggcacccac accttgcccc 1140
ggcccctgct cacctgccaa gccaggctca tctgtatacg tatgctgccc cgacttctgc 1200
tgctgcactg ggctcaacca gctccattgc tcatcttttc tccccacagg gttcctcaag 1260
gcatgctgca gcctatacca ctcaccctag cactttggtg caccaggtcc ctgtcagtgt 1320
tgggcccagc ctcctcactt ctgccagcgt ggcccctgct cagtaccaac accagtttgc 1380
cacccaatcc tacattgggt cttcccgagg ctcaacaatt tacactggat acccgctgag 1440
tcctaccaag atcagccagt attcctactt atagttggtg agcatgaggg aggaggaatc 1500
atggctacct tctcctggcc ctgcgttctt aatattgggc tatggagaga tcctccttta 1560
ccctcttgaa atttcttagc cagcaacttg ttctgcaggg gcccactgaa gcagaaggtt 1620
tttctctggg ggaacctgtc tcagtgttga ctgcattgtt gtagtcttcc caaagtttgc 1680
cctattttta aattcattat ttttgtgaca gtaattttgg tacttggaag agttcagatg 1740
cccatcttct gcagttacca aggaagagag attgttctga agttaccctc tgaaaaatat 1800
tttgtctctc tgacttgatt tctataaatg cttttaaaaa caagtgaagc ccctctttat 1860
ttcattttgt gttattgtga ttgctggtca ggaaaaatgc tgatagaagg agttgaaatc 1920
tgatgacaaa aaaagaaaaa ttactttttg tttgtttata aactcagact tgcctatttt 1980
attttaaaag cggcttacac aatctccctt ttgtttattg gacatttaaa cttacagagt 2040
ttcagttttg ttttaatgtc atattatact taatgggcaa ttgttatttt tgcaaaactg 2100
gttacgtatt actctgtgtt actattgaga ttctctcaat tgctcctgtg tttgttataa 2160
agtagtgttt aaaaggcagc tcaccatttg ctggtaactt aatgtgagag aatccatatc 2220
tgcgtgaaaa caccaagtat tctttttaaa tgaagcacca tgaattcttt tttaaattat 2280
tttttaaaag tctttctctc tctgattcag cttaaatttt tttatcgaaa aagccattaa 2340
ggtggttatt attacatggt ggtggtggtt ttattatatg caaaatctct gtctattatg 2400
agatactggc attgatgagc tttgcctaaa gattagtatg aattttcagt aatacacctc 2460
tgttttgctc atctctccct tctgttttat gtgatttgtt tggggagaaa gctaaaaaaa 2520
cctgaaacca gataagaaca tttcttgtgt atagctttta tacttcaaag tagcttcctt 2580
tgtatgccag cagcaaattg aatgctctct tattaagact tatataataa gtgcatgtag 2640
gaattgcaaa aaatatttta aaaatttatt actgaattta aaaatatttt agaagttttg 2700
taatggtggt gttttaatat tttacataat taaatatgta catattgatt agaaaaatat 2760
aacaagcaat ttttcctgct aacccaaaat gttatttgta atcaaatgtg tagtgattac 2820
acttgaattg tgtacttagt gtgtatgtga tcctccagtg ttatcccgga gatggattga 2880
tgtctccatt gtatttaaac caaaatgaac tgatacttgt tggaatgtat gtgaactaat 2940
tgcaattata ttagagcata ttactgtagt gctgaatgag caggggcatt gcctgcaagg 3000
agaggagacc cttggaattg ttttgcacag gtgtgtctgg tgaggagttt ttcagtgtgt 3060
gtctcttcct tccctttctt cctccttccc ttattgtagt gccttatatg ataatgtagt 3120
ggttaataga gtttacagtg agcttgcctt aggatggacc agcaagcccc cgtggaccct 3180
aagttgttca ccgggattta tcagaacagg attagtagct gtattgtgta atgcattgtt 3240
ctcagtttcc ctgccaacat tgaaaaataa aaacagcagc ttttctcctt taccaccacc 3300
tctacccctt tccattttgg attctcggct gagttctcac agaagcattt tccccatgtg 3360
gctctctcac tgtgcgttgc taccttgctt ctgtgagaat tcaggaagca ggtgagagga 3420
gtcaagccaa tattaaatat gcattctttt aaagtatgtg caatcacttt tagaatgaat 3480
ttttttttcc ttttcccatg tggcagtcct tcctgcacat agttgacatt cctagtaaaa 3540
tatttgcttg ttgaaaaaaa catgttaaca gatgtgttta taccaaagag cctgttgtat 3600
tgcttaccat gtccccatac tatgaggaga agttttgtgg tgccgctggt gacaaggaac 3660
tcacagaaag gtttcttagc tggtgaagaa tatagagaag gaaccaaagc ctgttgagtc 3720
attgaggctt ttgaggtttc ttttttaaca gcttgtatag tcttggggcc cttcaagctg 3780
tgaaattgtc cttgtactct cagctcctgc atggatctgg gtcaagtaga aggtactggg 3840
gatggggaca ttcctgccca taaaggattt ggggaaagaa gattaatcct aaaatacagg 3900
tgtgttccat ccgaattgaa aatgatatat ttgagatata attttaggac tggttctgtg 3960
tagatagaga tggtgtcaag gaggtgcagg atggagatgg gagatttcat ggagcctggt 4020
cagccagctc tgtaccaggt tgaacaccga ggagctgtca aagtatttgg agtttcttca 4080
ttgtaaggag taagggcttc caagatgggg caggtagtcc gtacagccta ccaggaacat 4140
gttgtgtttt ctttattttt taaaatcatt atattgagtt gtgttttcag cactatattg 4200
gtcaagatag ccaagcagtt tgtataattt ctgtcactag tgtcatacag ttttctggtc 4260
aacatgtgtg atctttgtgt ctcctttttg ccaagcacat tctgattttc ttgttggaac 4320
acaggtctag tttctaaagg acaaattttt tgttccttgt cttttttctg taagggacaa 4380
gatttgttgt ttttgtaaga aatgagatgc aggaaagaaa accaaatccc attcctgcac 4440
cccagtccaa taagcagata ccacttaaga taggagtcta aactccacag aaaaggataa 4500
taccaagagc ttgtattgtt accttagtca cttgcctagc agtgtgtggc tttaaaaact 4560
agagattttt cagtcttagt ctgcaaactg gcatttccga ttttccagca taaaaatcca 4620
cctgtgtctg ctgaatgtgt atgtatgtgc tcactgtggc tttagattct gtccctgggg 4680
ttagccctgt tggccctgac aggaagggag gaagcctggt gaatttagtg agcagctggc 4740
ctgggtcaca gtgacctgac ctcaaaccag cttaaggctt taagtcctct ctcagaactt 4800
ggcatttcca acttcttcct ttccgggtga gagaagaagc ggagaagggt tcagtgtagc 4860
cactctgggc tcatagggac acttggtcac tccagagttt ttaatagctc ccaggaggtg 4920
atattatttt cagtgctcag ctgaaatacc aaccccagga ataagaactc catttcaaac 4980
agttctggcc attctgagcc tgcttttgtg attgctcatc cattgtcctc cactagaggg 5040
gctaagcttg actgccctta gccaggcaag cacagtaatg tgtgttttgt tcagcattat 5100
tatgcaaaaa ttcactagtt gagatggttt gttttaggat aggaaatgaa attgcctctc 5160
agtgacagga gtggcccgag cctgcttcct attttgattt tttttttttt taactgatag 5220
atggtgcagc atgtctacat ggttgtttgt tgctaaactt tatataatgt gtggtttcaa 5280
ttcagcttga aaaataatct cactacatgt agcagtacat tatatgtaca ttatatgtaa 5340
tgttagtatt tctgctttga atccttgata ttgcaatgga attcctactt tattaaatgt 5400
atttgatatg ctagttattg tgtgcgattt aaactttttt tgctttctcc ctttttttgg 5460
ttgtgcgctt tcttttacaa caagcctcta gaaacagata gtttctgaga attactgagc 5520
tatgtttgta atgcagatgt acttagggag tatgtaaaat aatcatttta acaaaagaaa 5580
tagatattta aaatttaata ctaactatgg gaaaagggtc cattgtgtaa aacatagttt 5640
atctttggat tcaatgtttg tctttggttt tacaaagtag cttgtatttt cagtattttc 5700
tacataatat ggtaaaatgt agagcaattg caatgcatca ataaaatggg taaattttct 5760
g 5761
<210>3
<211>2129
<212>DNA
<213>人
<400>3
ctgtattgag acaaaggaag ggatctgtca gaaagcaaca cttgttatct tgggcttggc 60
agcaaggaag aggacaggta gtggagatcc tgcaatctga aaagcagact gaaaggtgac 120
aaagaagctg aagatgggtg gtggagagag gtataacatt ccagcccctc aatctagaaa 180
tgttagtaag aaccaacaac agcttaacag acagaagacc aaggaacaga attcccagat 240
gaagattgtt cataagaaaa aagaaagagg acatggttat aactcatcag cagctgcctg 300
gcaggccatg caaaatgggg ggaagaacaa aaattttcca aataatcaaa gttggaattc 360
tagcttatca ggtcccaggt tactttttaa atctcaagct aatcagaact atgctggtgc 420
caaatttagt gagccgccat caccaagtgt tcttcccaaa ccaccaagcc actgggtccc 480
tgtttccttt aatccttcag ataaggaaat aatgacattt caacttaaaa ccttacttaa 540
agtacaggta taaaataaga caaatgttta aatttagtta tgttcacggg tagttgtcaa 600
ttggtctgaa acaaatttgc tagggaatct atttgtgtag aactaattaa tgtaaaaaaa 660
atagaccatc tcgtgttgtg tgcactgtga tataatggta gtatcagtgc aacttaaact 720
aatgattgta cttgatatta agtgttctca actgagtaac ttttaagtgg aaaccaagtt 780
tagatttggg gagtggtaaa ggaatcagct ttttctattg ttaggggaag acagtaattt 840
atcattcatg gaccagtaga ttgttgaaag ttggtgaatc ggattataag cttctagcta 900
acacaaggat tcagaattag gtaaacatct gaaggtttag tatattagaa acacccaaac 960
cagtaatatg ctaacctgat gcactgctga aagaaaatgt gaatttttcg taataattgc 1020
attttagtga attgtacagt gggtggaaag ggcatttgga gctcattaga atgagacata 1080
gtacacccca atggccctgt ttattaaatg tagtggatta agtgtctgtc aacaaataca 1140
ccaaaaccat tttttataga aacagtattt aatggtcact caatagcttt caaaatacat 1200
ttttgtatta cagcactgca caagctattc taatagtgct ctggcctcat cattcctgca 1260
aagcttgctt tggggagttg gataatgtga aaattttaag tacctagggg agaaagagcc 1320
atgtaaatat ctgtaataaa cttgtagcat atgtaaagtt ttcttggcct ttatcttaca 1380
aaaatggagt attttagtat gaatttgctg aatgtaagac cgtggactgt tttttataat 1440
atggcctaat tttaaaggtc caaaataact tgtttttaaa gtttgccctt gtgctaaagt 1500
gccagtgtat gtatgttata cttgatttgg ttgtaaacta tatttcaaag taaaccctag 1560
tgtaataagt tttataacta aaaaggttta agctgctaaa actattttta agagatgtga 1620
aatgcagtat gggactatct ttttttcctc ctctaagccc aaagattaac tagagtccct 1680
ccaaccttat agattgttgg ctttcacaat cttataacct aggatacagg tagtttcgag 1740
tatggtgcca gtgatgtttt gtttttgttt ggtcaagggg taggtgcaac ccaatggacc 1800
acttatgcaa aagatgtaaa ctcttgcata atacattgat aacatgtttt gccaacttta 1860
aatgcttaaa cataagcgaa accagtagca agtatgtggg tcagcttaaa aattttgatt 1920
gttaatgccc tattttctaa tttggcacct cttgatgcct aagcaggtaa gcagatgcct 1980
aagctgtatt tctccaaata aatcaagatg aagtactgcc caagttaaat attgatagcc 2040
taaagacaag tttatgtagt acttaatgta catgatatga agcataaaat taaataaaat 2100
ttttccccat tgaaaaaaaa aaaaaaaaa 2129
<210>4
<211>3950
<212>DNA
<213>人
<400>4
cgagaactag ttttgttccg tgccctctgg actggaacct tttggagaga acccccggca 60
ggaccaaccc cgcacccgcc agcaccgcgg caatgtccag caatagtttt ccttacaatg 120
agcagtccgg aggaggggag gcgacggagc tgggtcagga ggcgacctca accatttccc 180
cctcgggggc cttcggcctc tttagcagcg atttgaagaa gaatgaagat ctaaagcaaa 240
tgttagagag caacaaagat tctgctaaac tggatgctat gaagcggatt gttgggatga 300
ttgcaaaagg gaaaaatgca tctgaactgt ttcctgctgt tgtgaagaat gtggccagta 360
aaaatattga gatcaagaag ttggtatatg tttacctggt tcgatatgct gaagaacagc 420
aggatcttgc actcctgtcc ataagcactt ttcagcgagc tctgaaggac ccaaaccaac 480
taattcgtgc aagcgctttg agagttctgt caagtattag agtgccaatt attgtaccta 540
tcatgatgct tgctattaag gaagcttctg ctgacttatc accatatgtt aggaagaatg 600
cagcccatgc aatacaaaaa ttatacagcc ttgatccaga gcagaaggaa atgttaattg 660
aagtaattga aaaacttctg aaagataaaa gcacattggt agctggcagt gttgtgatgg 720
cttttgaaga agtatgcccg gacagaatag atctgattca taaaaattac cgcaagctat 780
gtaacttact agtggatgtt gaagagtggg ggcaggttgt cataatccac atgctaactc 840
gatatgctcg gacacagttt gtcagccctt ggaaagaggg tgatgaatta gaagacaatg 900
gaaagaattt ctacgaatct gatgatgatc agaaggaaaa gactgacaaa aagaagaagc 960
cgtatactat ggatccagat catagactct taattagaaa tacaaagcct ttgcttcaga 1020
gcaggaatgc tgcggtggtt atggcagttg ctcagctgta ttggcacata tcaccaaaat 1080
ctgaagctgg cataatttct aaatcactag tgcgtttact tcgtagcaat agggaggtgc 1140
agtatattgt cctacaaaat atagcaacta tgtcaattca aagaaagggg atgtttgaac 1200
cttatctgaa gagtttctat gttaggtcaa ctgatccaac tatgatcaag acactgaagc 1260
ttgaaatttt gacaaacttg gcaaatgaag ccaacatatc aactcttctt cgagaatttc 1320
agacctatgt gaaaagccag gataaacaat ttgcagcagc cactattcag actataggca 1380
gatgtgcaac caacatcttg gaagtcactg acacgtgcct caatggcttg gtctgtctgc 1440
tgtccaacag ggatgaaata gttgttgctg aaagtgtggt tgttataaag aaattactgc 1500
aaatgcaacc tgcacaacat ggtgaaatta ttaaacatat ggccaaactc ctggacagta 1560
tcactgttcc tgttgctaga gcaagtattc tttggctaat tggagaaaac tgtgaacgag 1620
ttcctaaaat tgcccctgat gttttgagga agatggctaa aagcttcact agtgaagatg 1680
atctggtaaa actgcagata ttaaatctgg gagcaaaatt gtatttaacc aactccaaac 1740
agacaaaatt gcttacccag tacatattaa atctcggcaa gtatgatcaa aactacgaca 1800
tcagagaccg tacaagattt attaggcagc ttattgttcc gaatgaaaag agtggagctt 1860
taagtaaata tgccaaaaaa atattcctag cacaaaagcc tgcaccactg cttgagtctc 1920
cttttaaaga tagagatcat ttccagcttg gcaccttatc tcatactctc aacattaaag 1980
ctactgggta cctggaatta tctaattggc cagaggtggc gcccgaccca tcagttcgaa 2040
atgtagaagt aatagagttg gcaaaagaat ggaccccagc aggaaaagca aagcaagaga 2100
attctgctaa gaagttttat tctgaatctg aggaagagga ggactcttct gatagtagca 2160
gtgacagtga gagtgaatct ggaagtgaaa gtggagaaca aggcgaaagt ggggaggaag 2220
gagacagcaa tgaggacagc agtgaggact cctccagtga gcaggacagt gagagtggac 2280
gggagtcagg cctagaaaac aaaagaacag ccaagaggaa ctcaaaagcc aaaggaaaaa 2340
gtgattctga agatggggag aaggaaaatg aaaaatctaa aacttcagat tcttcaaatg 2400
acgaatctag ttcaatagaa gacagttctt ccgattctga atcagagtca gaacctgaaa 2460
gtgaatctga atccagaaga gtcactaagg agaaagaaaa gaaaacaaag caagatagaa 2520
ctcctcttac caaagatgtt tcacttctag atctggatga ttttaaccca gtatccactc 2580
cagttgcact tcccacacca gctctttctc caagtttgat ggctgatctt gaaggtttac 2640
acttgtcaac ttcctcttca gtcatcagtg tcagtactcc tgcatttgta ccaacgaaaa 2700
ctcacgtgct gcttcatcga atgagtggaa aaggactagc tgcccattat ttctttccaa 2760
gacagccttg catttttggt gataagatgg tctctataca aataacactg aataacacta 2820
ctgatcgaaa gatagaaaat atccacatag gggaaaaaaa acttcctata ggcatgaaaa 2880
tgcatgtttt taatccaata gactctcttg agcctgaggg atccattaca gtttcaatgg 2940
gtattgactt ttgtgattct actcagactg ccagtttcca gttgtgtacc aaggatgatt 3000
gcttcaatgt taatattcag ccacctgttg gagaactgct tttacctgtg gccatgtcag 3060
agaaagattt taagaaagag caaggagtgc taacaggaat gaatgaaact tctgctgtaa 3120
tcattgctgc accacagaat ttcactccct ctgtgatctt tcagaaggtt gtaaatgtag 3180
ccaatgtagg tgcagtccct tctggccagg ataatataca caggtttgca gctaaaactg 3240
tgcacagtgg gtcattgatg ctagtcacag tggaactgaa ggaaggctct acagcccagc 3300
ttatcataaa cactgagaaa actgtgattg gctctgttct gctgcgggaa ctgaagcctg 3360
tcctgtctca ggggtaacct gcttacatct ggactttaga atctggcaca caacaaaagt 3420
gcctggcatc cactactgct gcctttcatt tataataata gcccttccat ctggcagtgg 3480
gggtagaata cactcttgac attcttgtct cctgctttag aatgctagtg tgtatctatc 3540
atgtatgcaa tactttcccc ctttttgctt tgctaaccga agagcatata ttttactgtc 3600
agttgtctca actcttgaat ccatgtggcg ttttctctgt cctgctgctt cttttggcct 3660
cctcgttttc cttctctttt tcgacaatgg tagacatgaa tgagatattt aaagttcatt 3720
ggaaatcttc ttccctacag cagtaagcaa aaattagcaa agagatagtc taaatggcct 3780
ctcagcttgg tatgtgaaaa tgagatcaca tactttttaa atccaaatac aaaagcatag 3840
tctctgcaag attttgttct ttgaatttct tgatattgta attgattatt gataactgtc 3900
atcatgaaat tatctctcaa taataagata aataaactag catatgaatc 3950
<210>5
<211>5191
<212>DNA
<213>人
<400>5
gagaaagaaa aacagctcga gacctcatgc aaagagaaaa ctgagtatct acagaaaatg 60
gttcagagga atgaaagata taaacaagat gtggagaggt tctatgaacg gaagcgacat 120
ttagatttaa ttgagatgct tgaagcaaaa aggccatggg tggaatatga aaatgttcgt 180
caggaatatg aagaagtaaa actagttcgt gaccgagtga aggaagaggt cagaaaactt 240
aaagaagggc agattcctat aacatgtcga attgaagaaa tggaaaacga gcgtcacaat 300
ttggaggctc gaatcaaaga aaaggcaaca gatattaagg aggcatctca aaaatgcaaa 360
cagaagcaag atgttataga aaggaaagat aaacatattg aggaacttca gcaggcttta 420
atagtaaagc aaaatgaaga gcttgaccga cagaggagaa taggtaatac ccgcaaaatg 480
atagaggatt tgcaaaatga actaaagacc acggaaaact gcgagaatct tcagccccag 540
attgatgcca ttacaaatga tctgagacgg attcaggatg aaaaggcatt atgtgaaggc 600
gaaataattg ataagcgaag agagagggaa actctagaga aggagaaaaa gagtgtggac 660
gatcatattg tacgttttga caatcttatg aatcagaagg aagataagct aagacagaga 720
ttccgtgaca cgtatgatgc tgttttatgg ctaagaaata acagagacaa atttaaacaa 780
agagtctgtg agcccataat gctcacgatc aatatgaaag ataataaaaa tgccaaatat 840
attgaaaatc atattccatc aaatgactta agagcctttg tatttgaaag tcaagaagat 900
atggaggttt tcctcaaaga ggttcgtgac aataaaaaat taagagtaaa tgctgttatt 960
gctcccaaga gttcatatgc agacaaagca ccttcaagat ctttgaatga acttaaacaa 1020
tacggatttt tctcttattt gagagaatta tttgatgcac ctgatcctgt aatgagttac 1080
ctttgctgtc agtatcatat tcatgaagtt cctgtaggaa ctgaaaagac cagagaaaga 1140
attgaacggg taatacaaga aacccgatta aaacagattt atacagcaga agaaaagtat 1200
gtggtgaaaa cttcttttta ttcaaacaaa gttatttcta gtaacacatc tctaaaagta 1260
gcgcagtttc tcactgtcac tgtggaccta gagcagagaa gacacttaga agaacagcta 1320
aaggaaattc atagaaaatt gcaagcagtg gattcagggt tgattgcctt acgtgaaaca 1380
agcaaacatc tggagcacaa agacaatgaa cttagacaaa agaagaagga gcttcttgag 1440
agaaaaacca agaaaagaca actggaacaa aaaatcagtt ccaaactagg aagtttaaag 1500
ctgatggaac aggatacttg caatcttgaa gaggaagagc gaaaagcaag taccaaaatc 1560
aaagaaataa atgttcaaaa agcgaaactt gttaccgaat taacaaacct aataaagatt 1620
tgtacttctt tgcatataca aaaagtagat ttaattctcc aaaatactac agtgatctct 1680
gagaagaaca aattagaatc agattatatg gccgcatctt cacaactccg tcttacagag 1740
caacatttca ttgaattgga tgaaaataga cagagattat tgcagaaatg caaggaactt 1800
atgaaaagag ctaggcaagt atgtaacctg ggtgcagagc agactcttcc tcaagaatac 1860
cagacacaag tacccaccat tccaaatgga cacaactcct cactccccat ggttttccaa 1920
gaccttccaa acacattgga tgaaattgat gctttattaa ctgaagaaag atcaagagct 1980
tcctgcttca cgggactgaa tcctacaatt gttcaggaat atacaaaaag agaagaagaa 2040
atagaacagt taactgagga actaaaggga aagaaagttg aactagatca atacagggaa 2100
aacatttcac aggtaaaaga aaggtggctt aatcctttaa aagagctggt agaaaaaatt 2160
aatgaaaaat tcagcaattt ttttagttcc atgcagtgtg ctggtgaagt tgatctccat 2220
acagaaaatg aggaagatta tgataaatat ggaattcgaa ttagagtcaa atttcgaagt 2280
agtactcaac tgcatgaatt aactcctcat catcaaagtg gaggtgaaag aagtgtttct 2340
accatgttat acttgatggc acttcaggag ctaaatagat gtccattcag agtagttgat 2400
gaaatcaatc agggaatgga cccaatcaat gaacggagag tgtttgaaat ggttgtaaat 2460
actgcctgta aagaaaatac atctcaatac tttttcataa caccaaagct cctgcaaaat 2520
cttccttatt ctgaaaagat gacagttttg tttgtctaca atggccctca tatgctggaa 2580
ccaaacacat ggaatttaaa ggctttccaa aggcggcggc gccgtattac attcactcaa 2640
ccttcttaat aaaagtaaag agagggaact tgggaatttt ttttgttaaa ttctgtttat 2700
aagtatggct caactgaata aaaggagatt cactaaaacg aaaagcagtt atttttggaa 2760
acctgctttt aaatacaaat aggttgataa tggaaactat aatgaccttt ccaaaatagc 2820
agctggtagt aaaagttaag tcttcttcag tcttggttga acttgagttc ttggcactct 2880
gaccatgagt cattcagttc tcatgttaaa atgtacttaa tattacaaat caaaggtaca 2940
gtggaagaag ggttaatcac aagaagttac ttatatggta gccctgagct ttaattgcag 3000
agtaacttta attactttta gagcctaaag atgactctag agcctaagtc ctagtttctc 3060
ccaatgttat atttaatttt aaaaaattga tatgaaaatg tctaatgtat agtaataatt 3120
tatgacagat ctagtcattt cttcctatta aaaaagatta ccttatctcc agtaggaaat 3180
ggaattttat gggcctttaa aagaaagttt tatgaaactt gatgctataa ttttattggt 3240
atttcaaggg gaaaaaagca ctggggttca aaaatggtag cagaactgct ttgaaatgct 3300
gcaaggtggc cactagatga tgcaaaatac aaccaaaaga ttgactgaga ataaaattag 3360
gtgacaaggg tttttaaaga ataacctttt aaagtgtggg ggcaggggtt gctttttttt 3420
attttattta aagtcaatta tattttacat cttacatttc taaaagcatt ttataattat 3480
ttttagtaag atttttctta aaatttcata tactggtttc tacaatttat atttgaaatt 3540
tctcagtgtt atgtaaagag tgatggaaaa gcattgattt ctttaaaacc gtaatgtttt 3600
tagaacttaa gcctataggg cctttcttac aatgttgatg tacccattat cttagaaaat 3660
ctagtttaaa ctgttttctt tcaccgcaaa agaattaaat gggaaaatca tttgtttatc 3720
tctaagttat actaattagt agaaccaaac aaattatctt cttttaaaaa ataaatctta 3780
taggaaaata gacagtccaa agtcatgtct ttgaacagtg gattggatct gtgccagtaa 3840
tgacaaaatt atttttttga cttgcttgcc tgaataaatt gaagaattgc tttcagtttg 3900
ggttttgtat attcttaagt agccattgaa atttatattc ttaactaggt caaaaaataa 3960
tgagccataa gtttatgtcc tctcacttag acattttctc tttaaaaagg tattttcttc 4020
tttataaaca ttttaaaaga gccttccctt cttaaactaa ctccagtgca tgaagtgtga 4080
aaatatttta aaatgacatt tttactaata tgagcaagtc atgtaaacat tgaagaactt 4140
ggtaacatat tagtaaatgg atattaccaa atgttttcat cgttaattac tttgcgttcc 4200
accaaaatat ctttactaaa atgtgcttgg tgtagtttgt ttattgtcta aattagtacc 4260
agtcatctta tttctgcaaa atgagtatca atgtgaaaaa gacacgtgaa gattaagcat 4320
gtttgaaaat aaaatggtca attacatttc aatttacata ggccaacaac tgttccatac 4380
tttgtttgta aacatttaat ttctctactg gacaaaatta atatttggct ttacattgaa 4440
ttttgagctg tgaagaataa attatgtatc attttagcat attaaacagt agtaagtcta 4500
gcacatagtc tcagccactt aaaacaaaag tttttttgtt tgtttgtttg tttgtttttt 4560
tgagatggag tctcactctg ttgcccaggc tggagtgcag tggcgtgatc tcggcttact 4620
gcaacctccg cctcccgggt tcaagcgatt ctcctgcctc agcctcccaa gtaactggga 4680
caacaggcgc gtcccaccac acccagctaa ttttttatac ttttagtaga gatggggttt 4740
cagcatattg gccaggctgg tctcgaactc ctgaccttgt gatccacccg cctcggcctc 4800
ccaaagtgct gggattatag gcgtgagccc ctgcacccgg ccaaaagttg atttttaatt 4860
acataaaaat cgtaaaaact tctagtaaaa acttgatttg gtgaatacag ttatatttta 4920
aaaccttaag gtgacaagca ttttctatgc ctaaatcttc attggtttgc ctggaaagag 4980
tctctgttaa aagattttcc atattcaaag taaaaggaaa gatttcttgc ttcctaattg 5040
tcttttggac acatgcctat tttctttgag gtataaacct ttagatgtga aaaatgtaat 5100
ttcattctgc tattgtgtgt gcttgtgtgt gtgtaattga aaaaactggg aaatcctgct 5160
ttgttggtaa taaatcaata tttttatatt c 5191
<210>6
<211>4755
<212>DNA
<213>人
<400>6
aagagatctt ccaggctctc agagccctgg gagggcgatt tccaggaaga ccacaatgcc 60
aacctctgga ggaggctgga gagagaaggc ctaggccaga gcctgtcagg caactttggc 120
aagaccaagt cagccttctc atctctccag aacattcctg agagtctgag aagacacagc 180
agcctggagc taggccgggg aacccaggag ggttaccccg ggggcaggcc cacctgtgca 240
gtcaacacca aggcagaaga ccctgggagg aaagccgctc ctgacctcgg gagccatctg 300
gaccggcagg tttcctaccc gcggcccgag gggaggaccg gtgcctcggc ttctttcaac 360
agcacagacc caagtcccga agagccgcct gccccctcgc acccgcacac atccagtctg 420
ggccggaggg ggcccggccc aggcagcgcc tcggctcttc agggctttca gtacgggaag 480
ccccactgct cggtgctgga gaaggtctcc aaattcgagc agcgagagca agggagccag 540
agaccgagtg tgggcggctc tggttttggc cataactata ggccccacag gaccgtctca 600
acttccagta cttctgggaa tgacttcgag gagacaaaag cacacattcg tttctctgag 660
tcagctgaac ccctaggcaa cggggagcag cacttcaaaa acggggagct gaagttggaa 720
gaggcttccc ggcagccctg cggtcagcag ctgagcggag gagcgtcgga cagcggccgt 780
ggcccccaga ggccggacgc tcggctcctc cgtagccaga gcaccttcca gctctccagc 840
gagccagaga gggagcccga gtggcgggac aggcccggct cgcccgaatc gcccctgctg 900
gatgccccct tcagccgcgc ctaccggaac agcatcaagg acgcacagtc ccgtgtcttg 960
ggggccacct cctttcgacg tcgagacctg gagctggggg cgcccgtggc gtcgaggtcc 1020
tggcggccac ggccttcctc ggcccacgtg gggctgcgga gccccgaggc gtcggcctcc 1080
gcctccccgc acacgccccg ggagcggcac agcgtgaccc ctgctgaggg cgacctggcc 1140
aggcccgtgc cccctgccgc ccggagaggt gctcgccggc gcctgactcc cgagcagaag 1200
aagcgctcct actcggagcc cgagaagatg aacgaggtgg ggatcgtgga ggaggccgaa 1260
ccggcacccc tgggcccgca gagaaatggg atgcgtttcc cggagagcag cgtggccgac 1320
cggcgccgtc tcttcgagcg cgatggcaag gcctgctcca cgctcagcct gtcggggccc 1380
gagctgaagc agttccagca gagcgccctg gcggactaca tccagcgcaa gaccggcaag 1440
cggcctacct ccgccgccgg ctgcagcctc caggagcccg ggccactgcg tgagcgcgcc 1500
cagagtgcct acctccagcc cggccccgcg gcgctcgaag gctccggcct cgcctcggcc 1560
tccagcttga gctcactgcg ggagcccagc ctgcagcccc gcagggaggc cacgctcctg 1620
ccggccacag ttgcagaaac ccagcaggct ccccgagatc gcagcagctc cttcgccggt 1680
ggccgccgcc tcggggaacg gcgacgcggg gacctgctta gcggagcaaa cggtggaaca 1740
aggggcaccc agagagggga tgagaccccc agggagccat cctcctgggg ggccagggcc 1800
gggaagtcca tgtcggccga ggacctgctg gaacgctcgg acgtccttgc gggccctgtc 1860
catgtgaggt ccaggtcatc tcccgccacc gcagacaagc gccaggatgt gcttttgggg 1920
caagacagtg gctttggtct tgtgaaggat ccatgttatt tggctggtcc tggatctagg 1980
tcactcagtt gttcagaaag aggccaagaa gagatgctgc tgctcttcca ccatctcacc 2040
cctcgttggg gtggttcagg ctgcaaagcc attggtgatt cctccgttcc tagtgaatgt 2100
cctggaaccc tggaccatca gaggcaagcc agtaggacac cctgccccag gccaccactg 2160
gcaggaacgc aagggctggt cacagacacc agggctgcac ccctgacccc aattggcacc 2220
cctctgcctt cagccattcc ctctggctac tgctcacagg acggtcagac agggcgacag 2280
cctctcccgc cctacacccc tgccatgatg cacagaagca atggtcacac cctgacccag 2340
cctcccggtc caagaggctg tgagggcgat ggcccagagc atggggtaga agagggaacg 2400
aggaagaggg tctcgctgcc tcagtggcca cctccttctc gagcaaagtg ggcccacgca 2460
gccagagagg acagccttcc tgaggaatcc tcagcccctg attttgcaaa cctgaagcac 2520
tatcaaaaac agcagagtct tccaagttta tgcagcactt ctgacccaga cacacctctt 2580
ggggccccga gcactccagg gaggatctcc ctccgaatat ctgagtctgt cctgcgggac 2640
tccccgccac ctcatgagga ttatgaagac gaagtgtttg tgagggatcc gcaccccaag 2700
gccacgtcca gccccacatt tgaacctctt cccccacccc cacctcctcc accgagtcag 2760
gaaaccccgg tgtatagcat ggatgacttc cctccacctc ctccccacac tgtatgtgag 2820
gcgcagctgg acagtgagga tcccgagggg ccacgcccca gcttcaacaa actttctaaa 2880
gtgacaattg caagggaaag gcacatgcct ggtgcagccc atgtggtagg tagtcagaca 2940
ctggcttcca gactccaaac ttctatcaag ggttcagagg ctgagtccac accaccctcc 3000
ttcatgagcg ttcacgccca acttgctggg tctcttggtg ggcagccagc acccatccag 3060
actcaaagcc tcagccatga tccagtcagt ggaactcagg gtttagaaaa gaaagtcagt 3120
cctgatcctc agaagagttc agaagacatc agaacagagg ctttggccaa ggaaattgtc 3180
caccaagaca aatctctagc agacattttg gatccagact ccaggctgaa gacaacaatg 3240
gacctgatgg aaggtttgtt tccccgagat gtgaacttgc tgaaggaaaa cagtgtaaag 3300
aggaaggcca tacagagaac tgtcagctct tcaggatgtg aaggcaagag gaatgaagac 3360
aaggaagcag tgagcatgtt ggttaactgc cctgcctact acagtgtgtc tgctcccaag 3420
gctgagctac tgaacaaaat caaagagatg ccagcagaag tgaatgagga agaggaacag 3480
gcagatgtca atgaaaagaa ggctgagctc attggaagtc tcacccacaa gctggagacc 3540
ctccaggagg cgaaggggag cctgctcacg gacatcaagc tcaacaacgc cctgggagaa 3600
gaggtggagg ctctgatcag cgagctctgc aagcccaatg agtttgacaa gtataggatg 3660
ttcatagggg atttggacaa ggtggtcaac ctgctgctct ccctctcggg gcgtctagcc 3720
cgtgttgaga atgtccttag cggccttggt gaagatgcca gtaatgaaga aaggagctct 3780
ctttacgaga aaaggaagat cctggctggt cagcatgagg atgcccggga gctgaaggag 3840
aacctggatc gcagggagcg agtagtgctg ggcatcttgg ccaattacct ttcagaggag 3900
cagctccagg actaccagca cttcgtgaaa atgaagtcca cgctcctcat tgagcaacgg 3960
aagctggatg acaagatcaa gctgggccag gagcaggtca agtgtctgct ggagagcctg 4020
ccctcagatt tcattcccaa ggctggggcc ctggctctgc ccccaaacct cacgagtgag 4080
cccattcctg ctgggggctg tactttcagt ggtattttcc caacattaac ctctccactt 4140
taacctcttc taaaataccc aaccaaaaga tcactgtttc tctcaacact atttaatctg 4200
aaaaatgttt cagtacaaac cactgtttga actatctggg ttattggtgt ttgttcctga 4260
tgaaaggaaa aaaattctct ccaggaggaa gcctttttcc ttcttgccct tcctgattga 4320
tcttctgaga gctcgaatgc tgctggacac gtaccccttt ctattattac tttgtagtag 4380
aaagaaagtt aatgaaactg agaactgatt ggagggtgtt tgatcattta gtttttaaca 4440
ggctgaggca acatggatca gtgtgtgtcc ccctcaggaa tgtatccaca gtggccttcc 4500
ttgctggtgg gcagtgtatc ctgatggcag ggtacaagta ccattaatga agggtctgca 4560
acataaagcc ttaaaaagac acacactaag aaaactgtaa aaccttgaac attgttattt 4620
atatttttta aaatggaaaa gatcactatg tttgttgtgc taaccactta tttgattctg 4680
ttttgtggtg gacatagatg attacgtttg agctttgtat tttgtgaaaa ccttaatgaa 4740
atgaattcca aagat 4755
<210>7
<211>2045
<212>DNA
<213>人
<400>7
gaaacttgac cccggctcat cctgtctctg gctgtggccc ggcaaagcac tgaaaacccc 60
tctggtctca gagacagtag gggcagtgcc actttctaca acctgccaac ccacacactg 120
gagtaattct gaaaaaaatt attcctaaac tctctaagtg tggacggaga atgagcaagc 180
cccagaagta ttttacaacc agagtgggta atgaggaggg ggcttactgg aatcgtcata 240
tctctgaata ttgaaaacaa caactaaaaa agtggacctt ctcagaaaaa aagggcagca 300
aatgaccaag ggcgcccctt ctggccgtgc ttggcttgag taactgtctc tctttcccca 360
cccccatcac agggctttca gtttggcaaa ggaaaagcag ataaaaacag aacattccat 420
atgtttcttt ctccatcggc caaaaacatt ttgacacaat gtttgtgaaa cacctttgga 480
gaggtgcact tctgaatgct gcctctgccg taaatcctgg ggcaagggat cagcctcttc 540
ccaggaacca tcgccttcta taaaccgtga actcaagcag gcattttttt tttcttaccg 600
aaaggctgct attgtgcaag ggcacataat gggtctgttg ctcttattgg cttccaaatg 660
tgcatggcaa agagagagat gtgggcctag agcagatata ttcagcaagg tgacagcttc 720
ccataacaat tctaacactt cttatcttat gtgagaataa aatatttaag ggttgaacct 780
tattttgcca aatgtatctt ttctgctttt gaattgggca gaagatttta gcaactatat 840
tctacaaatg ttacttataa cacacacaca cacatctgaa atatatgccg aaaattgacg 900
tctttgacct cagggagagc acctgtccag gtctgcctaa aggaaatggc tccagtgggt 960
ctaaacaacc acatcctatc catggatagg tctagtcata acactttaga gagaatgtca 1020
gagcaggagg gaggcaagcc gcctcttctc ggccatcaac tgcagatgat gaaagagcgg 1080
gattcaactt tgttttcttt tcctgtggcc ccagtgaaac ctcctgccct ccctgcacgt 1140
ctgtgtcttc atttctaaaa tgggggtgat gctttcatat tgacctcacc ccatactacc 1200
tcacagatgt gttgtgagga ttaataaaat tatgtctatg gtattttcag tttctggaga 1260
aaaatactta tagacagttt aactattaca tagatatata agtgatctca gtttcttgtt 1320
tgctgtgata ctaatgtgtt gttttaactt attccataaa atgacagttg tgtcctagcc 1380
acatcagaca gctatctaag ctctggacta cccctttgtg cagctgaatc actgcagggt 1440
cgaccatgcc tggtgccaca gccatggttt ccatttctag atgaaaggat ggcctaggac 1500
ataggtctca aagactcttg gatcagaatc aggagattag ggaaaacagg atggatacct 1560
gagcactaac agcagtagac gtagacctct gtcctttacc atctgaggtc ttctggattc 1620
tttgtggggt taattttgat ttgatgtcat ctgtttgccc ttcatcttgc ttgcaagtgt 1680
gcatggttca atccctcaca tccaggaaat gaattttgca attgggccag atgctaattt 1740
gcacgttgat tcaccttctt tgcctttaag cctttttttt cttttttttt ttttttggca 1800
aatgaatgta ccatttcaac tttgatttta atagtgctag ttgatattgg taataatgct 1860
aaccaagaga tcaatgccag atttttctct tggggtaagt tagctgaagt catttaaaga 1920
tggaaaggtg ggaaaattct ttgatatttg atgtcattgt atccacattt gttgtaagac 1980
atattgcata ccaattataa ttatatcaat taaagttgat aaaagcttca aaaaaaaaaa 2040
aaaaa 2045
<210>8
<211>2096
<212>DNA
<213>人
<400>8
atggagaacg agcctgtagc ccttgaggaa actcagaaga cagatcctgc tatggaacca 60
cggttcaaag tggtggattg ggacaaggac ctggtggact ggcgaaagcc tctcctgtgg 120
caggtgggcc acttgggaga gaagtacgat gagtgggttc accagccggt gaccaggccc 180
atccgcctct tccactcaga cctcattgag ggcctctcta agactgtctg gtacagtgtc 240
cccatcatct gggtgcccct ggtgctgtat ctcagctggt cctactaccg aacctttgcc 300
cagggcaacg tccgactctt cacgtcattt acaacagagt acacggtggc agtgcccaag 360
tccatgttcc ccgggctctt catgctgggg acattcctct ggagcctcat cgagtacctc 420
atccaccgct tcctgttcca catgaagccc cccagcgaca gctattacct catcatgctg 480
cacttcgtca tgcacggcca gcaccacaag gcacccttcg acggctcccg cctggtcttc 540
ccccctgtgc cagcctccct ggtgatcggc gtcttctact tgtgcatgca gctcatcctg 600
cccgaggcag tagggggcac tgtgtttgcg gggggcctcc tgggctacgt cctctatgac 660
atgacccatt actacctgca ctttggctcg ccgcacaagg gctcctacct gtacagcctg 720
aaggcccacc acgtcaagca ccactttgca catcagaagt caggatttgg tatcagcact 780
aaattgtggg attactgttt ccacaccctc actccagaga aaccccacct gaagacgcag 840
tgacaactcc caccccctcc gtcctgccct cagcccggcc ctggcccctt cccgaccccc 900
acccgccatt cagaccccat taagaaggtt ggcttggcca ggcaggatgg gctgtgtccg 960
gccctgcagc ctagtggaag gtgctgaggg ggccctgagg caggaccgcc ctcctgaccc 1020
ctggtaggag ggtcacatcc acttggtgca ggtggccctt ggtgacccac ttcttcctgg 1080
agcgtccctg cctagagctc agcccacagg actgcttcag gccgtggcca caggtagcag 1140
ccgcaagggg aaatgaagaa aactgagccc tcgtggccac ctgtgtcacc cttgtgcctt 1200
agcctcatgg gctgcctagg agctgcctgc acggcacagc tcgctttcac agtcagaagt 1260
gggtctgtgg gatctgtggt ccctgtcctc cctgctgtcc cttctgggga ggctttggtg 1320
gctctgaggt ggacaaagag ctctcgcaag aagagacagc gtgatgcctc ccacagtcca 1380
ccccagaccc tggggcagcc cctctggccc tgccagctgc ctgcgtcgtt gggcccaggg 1440
tggctggcag gagtcccagc tgcttgcttt aggacctggc agcttttctt gccgtccctc 1500
ccctgcctcc agaatcacag cccttctccc caagggaggc tgaggaggct tctccaccag 1560
tggcagcccc accccgtccc tggccattct tggcctccac cccgctcagg cccctactcg 1620
ggcgctccca gaaggagcca cctctcagtg cctcacctcc ccctgcctcc cagcctccgc 1680
agatgaggtt cctgcccctt cctcctcgta accaaaaccc tcactgctcc caggacggtc 1740
ttatttataa accagataca tgttcttagt ctggtcccag accaaggagc tggtcagacg 1800
gccctttcta atcctacatg ttgagcttat gtaaaaaatg ttgtttcctc ctgtttttgg 1860
ttcctttctt acccacaaac cattactact tgaaacttaa aaaactcgcc aagtgtaaag 1920
gctaaagaga agcagtttga cggaccttgt gatttgtact gtttgctgcg gagctattta 1980
aagattttgg aataaatata caaaactacg gttgtgaaat aaaaacttaa attgtatatt 2040
ttgaaaaata aaacactgaa aagaaaccaa caaaaaaaaa aaaaaaaaaa aaaaaa 2096
<210>9
<211>5640
<212>DNA
<213>人
<400>9
ggaaacgcag aaaacagaga gaggcattct gagtcatctg actggatgaa gactgttcca 60
agttacaacc aaacaaatag ctccatggac tttagaaatt atatgatgag agatgagact 120
ctggaaccac tgcccaaaaa ctgggaaatg gcctacactg acacagggat gatctacttc 180
attgaccaca ataccaagac aaccacctgg ttggatcctc gtctttgtaa gaaagccaaa 240
gcccctgaag actgtgaaga tggagagctt ccttatggct gggagaaaat agaggaccct 300
cagtatggga catactatgt tgatcacctt aaccagaaaa cccagtttga aaatccagtg 360
gaggaagcca aaaggaaaaa gcagttagga caggttgaaa ttgggtcttc aaaaccagat 420
atggaaaaat cacacttcac aagagatcca tcccagctta aaggtgtcct tgttcgagca 480
tcactgaaaa aaagcacaat gggatttggt tttactatta ttggtggaga tagacctgat 540
gagttcctac aagtgaaaaa tgtgctgaaa gatggtcccg cagctcagga tgggaaaatt 600
gcaccaggcg atgttattgt agacatcaat ggcaactgtg tcctcggtca cactcatgca 660
gatgttgtcc agatgtttca attggtacct gtcaatcagt atgtaaacct cactttatgt 720
cgtggttatc cacttcctga tgacagtgaa gatcctgttg tggacattgt tgctgctacc 780
cctgtcatca atggacagtc attaaccaag ggagagactt gcatgaatcc tcaggatttt 840
aagccaggag caatggttct ggagcagaat ggaaaatcgg gacacacttt gactggtgat 900
ggtctcaatg gaccatcaga tgcaagtgag cagagagtat ccatggcatc gtcaggcagc 960
tcccagcctg aactagtgac tatccctttg attaagggcc ctaaagggtt tgggtttgca 1020
attgctgaca gccctactgg acagaaggtg aaaatgatac tggatagtca gtggtgtcaa 1080
ggccttcaga aaggagatat aattaaggaa atataccatc aaaatgtgca gaatttaaca 1140
catctccaag tggtagaggt gctaaagcag tttccagtag gtgctgatgt accattgctt 1200
atcttaagag gaggtcctcc ttcaccaacc aaaactgcca aaatgaaaac agataaaaag 1260
gaaaatgcag gaagtttgga ggccataaat gagcctattc ctcagcctat gccttttcca 1320
ccgagcatta tcaggtcagg atccccaaaa ttggatcctt ctgaggtcta cctgaaatct 1380
aagactttat atgaagataa accaccaaac accaaagatt tggatgtttt tcttcgaaaa 1440
caagagtcag ggtttggctt cagggtgcta ggaggagatg gacctgacca gtctatatat 1500
attggggcta ttattcccct gggagcagct gagaaagatg gtcggctccg cgcagctgat 1560
gaactaatgt gcattgatgg aattcctgtt aaagggaaat cacacaaaca agtcttggac 1620
ctcatgacaa ctgctgctcg aaatggccat gtgttactaa ctgtcagacg gaagatcttc 1680
tatggagaaa aacaacccga ggacgacagc tctcaggcct tcatttcaac acagaatgga 1740
tctccccgcc tgaaccgggc agaggtccca gccaggcctg caccccagga gccctatgat 1800
gttgtcttgc aacgaaaaga aaatgaagga tttggctttg tcatcctcac ctccaaaaac 1860
aaaccacctc caggagttat tcctcataaa attggccgag tcatagaagg aagtccggct 1920
gaccgctgtg gaaaactgaa agttggagat catatctctg cagtgaatgg gcagtccatt 1980
gttgaactgt ctcatgataa cattgttcag ctgatcaaag atgctggtgt caccgtcaca 2040
ctaacggtca ttgctgaaga agagcatcat ggtccaccat caggaacaaa ctcagccagg 2100
caaagcccag ccctgcagca caggcccatg ggacagtcac aggccaacca catacctggg 2160
gacagaagtg ccctagaagg tgaaattgga aaagatgtct ccacttctta cagacattct 2220
tggtcagacc acaagcacct tgcacagcct gacaccgcag taatttcagt tgtaggcagt 2280
cggcacaatc agaaccttgg ttgttatcca gtagagctgg agagaggccc ccggggcttt 2340
ggattcagcc tccgaggggg gaaggagtac aacatggggc tgttcatcct tcgtcttgct 2400
gaagatggtc ctgccatcaa agatggcaga attcatgttg gtgaccagat tgttgaaatc 2460
aatggggaac ctacacaagg aatcacacat actcgagcaa ttgagctcat tcaggctggt 2520
ggaaataaag ttcttcttct tttgaggcca ggaactggct tgatacctga ccatggtttg 2580
gctccttccg gtctgtgctc ctacgtgaaa cccgagcaac attaaggctt tcagggcttt 2640
tcttggtctt tccttaaaaa gacttggtga ttgggatatt aataatcctt cgtcttcaaa 2700
tgtgatttat gatgaacagt caccattacc cccatcttca cattttgctt ccatatttga 2760
agagtctcac gtgccagtaa ttgaagaatc tttgagagtt cagatatgtg aaaaggcaga 2820
agaattaaag gacattgtgc ctgaaaagaa aagcacttta aatgaaaatc agcctgagat 2880
aaagcatcag tctcttctcc agaaaaatgt gagtaagagg gatccaccca gcagtcatgg 2940
gcacagtaac aagaaaaatc tattaaaagt agaaaatggt gttacacgaa gaggtagatc 3000
ggttagtccc aaaaagccag ccagtcaaca ttcagaggaa catttggata agattcctag 3060
tcctctaaaa aataacccca aaagaagacc cagagatcaa tccctcagcc ccagcaaagg 3120
ggaaaataaa agttgtcagg tcagcaccag ggcaggctct ggacaagatc agtgcagaaa 3180
aagcagaggt cggtcggcca gcccaaaaaa gcagcaaaaa attgaaggaa gcaaagctcc 3240
atcaaatgct gaggccaaat tattagaggg taagagtcga agaatagcag gctatacggg 3300
cagtaatgct gagcagatcc cagatgggaa ggaaaaatca gacgtcatca ggaaagatgc 3360
aaagcagaat cagttggaaa aaagcagaac aaggtctcca gagaaaaaaa tcaaaagaat 3420
ggttgagaaa tctcttccat ccaaaatgac taataagact acaagtaaag aagtatctga 3480
aaatgaaaaa ggaaagaaag taaccacagg agaaacaagt tctagtaacg ataaaatagg 3540
agaaaatgtc cagctatcag aaaagaggct gaagcaagaa cctgaagaga aggtagtttc 3600
aaacaaaaca gaagatcaca aagggaaaga actagaggca gctgacaaaa acaaagagac 3660
tggaaggttc aaaccggaaa gcagttctcc agttaagaaa acactgataa ctccagggcc 3720
ctggaaggtt ccaagtggaa ataaagtcac aggcactatt ggtatggctg agaaacggca 3780
gtaaccttta gtataaaaca aagaaaaaca agttgtaatc ttttcttaca gcagcatttt 3840
tccagaaaaa gccttttttt ttttttcaga tattctgaaa cagataagta catgttaatg 3900
tgagcctcaa gttacctagg ctgcatgaag ggcctttagg attgctaaga accaactgtc 3960
cccctggccg gctgccctcc ctcgctctca ggaaggagct gcatccacat gctcatctga 4020
cccgccctgc tcaggctgcc cagctcgtct tcatgagtgt ctgaacaaat gacatatgtt 4080
gatattaaca atgtggtcac aactcacttt gtatttgtgc caagttatct actgtatcat 4140
gtctgttttt atcctttttg ttcagctgtt tccacagtaa tgaaaaagtt aggtttggct 4200
tggaagttga tgatctcaat agcatgttgc atgtttacag agagaaatat gtgagtcctt 4260
gcagaagaag agactgttaa ctcatcgtta aagatggccg ttgtctcttc taacagctac 4320
tgatgatgtc ccactttaaa aataaaaccc ccaaacatca ctactttaag gaaaaaaaaa 4380
atgtagtcca atattgatgc tttcttatgg ctttttattt taatttggct ggataagttg 4440
tttcaaataa ctgttaaaga tattacttac aattgaatgt ttgaaataag aaagtacttt 4500
aagcaataga gttcatctcc tgctgtgtta tccaacctcg atgtatactt acagcatctc 4560
aggtcaccct ttttatttca gttatttaat tatgaaacca taaagaagca tgtggaaata 4620
gtgtttattg ctctttgaag aaaaaccacc aactatttct ggatattttg gctgtaccta 4680
ctactaaagt cattagtctt taatacataa tacatatttg aaaagtaaac atattatata 4740
gattatgtga gggacttaat catgaaacca gtttcacagt ccaagtacca actcttctgg 4800
tagcaggtgc acaagcttgg gtgtttaaaa acaacctgtg tagggtatgc ccagcaaatg 4860
aggacaaatg tgtagacagt acttactgga tcttatttaa cttttagcta cattaactaa 4920
ctttcttatt taaaaacaag aaagggagac taaacatctg cttaacttgt acacattttc 4980
agaattcttt ttaaaagtct agttaaagat gtttcttaga agttggagac tgttaacaac 5040
ttccataaaa tagatccagg tttttcagtt ccctgaagca gcattcagta gcatctatat 5100
aaataaaggc accttctgag aataaaacta ttttatggag tgtgtgaaca cacttgttct 5160
gtcacctggg ttcatcttgt tgtgaagcac attaggtcca ggtccttccc tctgggagtc 5220
tgactgtgaa actctttaac ccaacaactc aattagcccc tgtagataag acatgcttcc 5280
cagagtgaga tttttgaaat ccccttttca tccagaacta tatttaccca cctattgtaa 5340
ctattcaaat agagcaaaat taggaggctt gataaatact aagaatttag taccacagaa 5400
attatttatt attttccctg tagtccacaa ttagtgataa cgaatcctat ttttgttaac 5460
tgtgacataa ctttgatgtc atatgttgtc ctatgtggtt cttcctaagt aaactctgta 5520
ctgattatat actgacttag caatgtggcc ttggaatgct gagcaaaatg tggatgtact 5580
ggttgtaaat gtttatatat tgtacagtac ctttatatat acacttgagg ttctgattag 5640
<210>10
<211>457
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(242)..(242)
<223>任意碱基
<220>
<221>misc_特征
<223>任意碱基
<220>
<221>misc_特征
<222>(394)..(394)
<223>任意碱基
<220>
<221>misc_特征
<222>(406)..(406)
<223>任意碱基
<220>
<221>misc_特征
<222>(457)..(457)
<223>任意碱基
<400>10
tcagtcactc tttcaccctg ccaaagcttc actgtcctac tgattgaatt gtatgtgaga 60
aataaaatgt catcatatta agccactggg atttgtatgt ttatctgtta tagcagcaag 120
tcttaattta cctaatacac acattgtgac agatgttctt aatgtcccac cccatattgt 180
tacatgtcca gctttgagga tccctggcat gtgggggtag gagtttctgg gcatgctgga 240
tncaattccc acttttaagg catctgtggc ctctgtggcc tctgtggcct tcactgttat 300
ggaagggatt tatctggggc accataggaa actttaccat ggcacagtgg acaacctagg 360
agggggtgng gaggaggggc cttcaggccc aacngggggg accagngttc gtggggttag 420
ggtggtttgg ggggttttcc ctcttacccg tgggggn 457
<210>11
<211>1493
<212>DNA
<213>人
<400>11
aatagggttg gcggctgcag cgggcggcaa acagcccgcc cggcaccacc atgctcgccc 60
tggaggctgc acagctcgac gggccacact tcagctgtct gtacccagat ggcgtcttct 120
atgacctgga cagctgcaag cattccagct accctgattc agagggggct cctgactccc 180
tgtgggactg gactgtggcc ccacctgtcc cagccacccc ctatgaagcc ttcgacccgg 240
cagcagccgc ttttagccac ccccaggctg cccagctctg ctacgaaccc cccacctaca 300
gccctgcagg gaacctcgaa ctggccccca gcctggaggc cccggggcct ggcctccccg 360
cataccccac ggagaacttc gctagccaga ccctggttcc cccggcatat gccccgtacc 420
ccagccctgt gctatcagag gaggaagact taccgttgga cagccctgcc ctggaggtct 480
cggacagcga gtcggatgag gccctcgtgg ctggccccga ggggaaggga tccgaggcag 540
ggactcgcaa gaagctgcgc ctgtaccagt tcctgctggg gctactgacg cgcggggaca 600
tgcgtgagtg cgtgtggtgg gtggagccag gcgccggcgt cttccagttc tcctccaagc 660
acaaggaact cctggcgcgc cgctggggcc agcagaaggg gaaccgcaag cgcatgacct 720
accagaagct ggcgcgcgcc ctccgaaact acgccaagac cggcgagatc cgcaaggtca 780
agcgcaagct cacctaccag ttcgacagcg cgctgctgcc tgcagtccgc cgggcctgag 840
cacacccgag gctcccacct gcggagccgc tgggggacct cacgtcccag ccaggatccc 900
cctggaagaa aaagggcgtc cccacactct aggtgatagg acttacgcat ccccaccttt 960
tggggtaagg ggagtgctgc cctgccataa tccccaagcc cagcccgggc ctgtctggga 1020
ttccccactt gtgcctgggg tccctctggg atttctttgt catgtacaga ctccctggga 1080
tcctcatgtt ttgggtgaca ggacctatgg accactatac tcggggaggc agggtagcag 1140
tgcttccaga gtcccaagag cttctctggg attttcttgt gatatctgat tccccagtga 1200
ggcctgggac ctttttaaga tcgctgtgtg tctgtaaacc ctgaatctca tctggggtgg 1260
gggccctgct ggcaaccctg agccctgtcc aaggttccct cttgtcagat ctgagatttc 1320
ctagttatgt ctggggccct ctgggagctg ttatcatctc agatctcttc gcccatctat 1380
ggctgtgttg tcacatctgt cccctcattt ttgagatccc ccaattctct ggaactattc 1440
tgctgcccct ttttatgtgt ctggagttcc ccaatcacat ctagggctcc tcc 1493
<210>12
<211>2292
<212>DNA
<213>人
<400>12
ccatgggttc cccttcagcc tgtccataca gagtgtgcat tccctggcag gggctcctgc 60
tcacagcctc gcttttaacc ttctggaacc tgccaaacag tgcccagacc aatattgatg 120
tcgtgccgtt caatgtcgca gaagggaagg aggtccttct agtagtccat aatgagtccc 180
agaatcttta tggctacaac tggtacaaag gggaaagggt gcatgccaac tatcgaatta 240
taggatatgt aaaaaatata agtcaagaaa atgccccagg gcccgcacac aacggtcgag 300
agacaatata ccccaatgga accctgctga tccagaacgt tacccacaat gacgcaggat 360
tctataccct acacgttata aaagaaaatc ttgtgaatga agaagtaacc agacaattct 420
acgtattctc ggagccaccc aagccctcca tcaccagcaa caacttcaat ccggtggaga 480
acaaagatat tgtggtttta acctgtcaac ctgagactca gaacacaacc tacctgtggt 540
gggtaaacaa tcagagcctc ctggtcagtc ccaggctgct gctctccact gacaacagga 600
ccctcgttct actcagcgcc acaaagaatg acataggacc ctatgaatgt gaaatacaga 660
acccagtggg tgccagccgc agtgacccag tcaccctgaa tgtccgctat gagtcagtac 720
aagcaagttc acctgacctc tcagctggga ccgctgtcag catcatgatt ggagtactgg 780
ctgggatggc tctgatatag cagccttggt gtagtttctg catttcggga agagtgtttt 840
tattatccac ctgcagactg gactggattc ttctagctcc ttcaatccca ttttctcctg 900
tggcatcact aagtataaga cctgctctct tcctgaagac ctataagctg gaggtggaca 960
actcaatgta aatttcaagg aaaaaccctc atgcctgaga tgtgggccac tcagagctaa 1020
ccaaaatgtt caacaccata actagagaca ctcaaattgc caaccaggac aagaagttga 1080
tgacttcatg ctgtggacag tttttcccaa gatgtcccaa gcctcatcgt gacgaggctc 1140
ttatcccact ccatttttcc ctgctcatgc ctgcctcttt aatttggtaa gataatgctg 1200
taactagaat ttcacaatca gcgccttgtg caggcaattt gacagagtgt tggatgtgtc 1260
atgtcatcat gtcaaaccca aatatttgac ctaagggatc ctttattctg cccagtggct 1320
aactttaaca acatccctaa tacaactgtt tattcaaatg cacggtggtc cctgttagag 1380
ttagacctct agactcacct gttctcacgc cctgttttaa tttaacccag ctatgggatg 1440
ccagataaca gaattgctgc ctacgagctg aacagggagg agtttgtgca gttgctgaca 1500
cttcttgttg cacataaata aatacagtgg gtactataga gactcagttg caaaaattaa 1560
caaatatgct gcttgattaa aatgggtagg cttctcatgt ggctcattct ttaatctatt 1620
ctcttttatt tggtttggtt catggggtct ctgcctatgg atcatacttc aaactcttgg 1680
tgtgatcctc ctgattgtca caatattagt taccctggtg tgctgtattc tctaaaacct 1740
ttaaatgttt gcatgcagcc attcgtcaaa tgtcaaatat tctctctttg gctggaatga 1800
caaaaactca aataaatgta tgattaggag gacatcataa cctatgaatg atggaagtcc 1860
aaaatgatgg taactgacag tagtgttaat gccttatgtt tagtcaaact ctcatttagg 1920
tgacagcctg gtgactccag aatggagcca gtcatgctaa atgccatata ctcacactga 1980
aacatgagga agcaggtaga tcccagaaca gacaaaactt tcctaaaaac atgagagtcc 2040
aggctgtctg agtcagcaca gtaagaaagt cctttctgct ttaactctta gaaaaaagta 2100
atatgaagta ttctgaaatt aaccaatcag tttatttaaa tcaatttatt tatattcttc 2160
tgttcctgga ttcccatttt acaaaaccca ctgttctact gttgtattgc ccagtaggag 2220
ctatcactat attttgcaga atggaaactg ccctgactct tgaatcacaa ataaaagcca 2280
attgtatctg tt 2292
<210>13
<211>519
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(212)..(212)
<223>任意碱基
<220>
<221>misc_特征
<222>(451)..(451)
<223>任意碱基
<400>13
gaaacaacaa cagtgtaatc tttaacaggg atgttaaagg taagaagtca ggaagataaa 60
ccaaaatgat tgagtatgat aaagaatttt gcatggcgat taaaatagaa aacctataaa 120
tgtagaaaaa gcaggtctgg acttagcaaa gaaacaatat agtttggaga aggcatgaaa 180
taagttcttt tcatgttcac tgctggtcac ancataacag agagtgatgt ggagagcttt 240
gggaaggttt cacgttgagt tacatcagtg gtcaacaatg gagcaacaag actccgtaga 300
ggatgccacc ctgggagaat tgcaagggaa aggaggctga agcacaactg gtaatagcct 360
tcagatattt aatggatatg caaataaagc tctgattaat tgtattttca cttattatat 420
atcatctttg gacctttcta aaagtgggac nctagaaaag atatactgaa actccaaaag 480
aatacttcag ctcgagttga atggattcaa gatgttgtt 519
<210>14
<211>5294
<212>DNA
<213>人
<400>14
ggctcgcatc cccatagtgc tgggttacag tgaaggtacg ccccgcgctc tgctctggag 60
aggcagggtg ggatagggaa cgtctcgagt ggcgcccgca gtcatggtgg tgttcgttgg 120
ccgccgcctc ccggcgctcc tagggctgtt taagaagaag ggctctgcca aggctgagaa 180
tgacaaacat ctaagtgtag ggcctggcca ggggccaggg tctgcagtgg atgagcacca 240
ggacaacgtc ttctttccca gtgggcgacc cccccacctg gaagagctgc acactcaggc 300
ccaggagggg ctccgctccc tacaacacca agagaaacag aaactgaaca agggtggctg 360
ggaccatgga gacacccaga gtatccagtc ctcccggacg gggccggatg aagacaacat 420
ctccttctgc agtcagacca catcctacgt ggctgagagc tccacagcag aggacgcgct 480
ctccatccgc tcggagatga tccagcgcaa aggctccacc ttccgacccc atgactcatt 540
tcccaaatct ggaaagtcag ggcggcgtcg gcgggagcgg cggagcactg tgctgggact 600
cccgcagcat gtgcagaagg agcttggcct gaggaatgag cgtgaggcac caggcacgcc 660
ccgggctcct ggtgcacggg atgccgtacg catccccaca gtggacggcc gcccccgagg 720
cacctcaggg atgggggccc gggtgtccct gcaggcgctg gaggcggagg cagaggctgg 780
cgctgagaca gaggccatgc tgcagcgcca cattgaccgt gtctaccggg atgacacctt 840
tgttggccgg tccacgggta cccgggcccc accattgacc cggcccatgt ccctagcagt 900
gcctggattg acaggagggg cagggcctgc agagcccctg agcccggcca tgtccatctc 960
cccccaggcc acctacctgt cgaagttgat tccacatgct gtgctgccgc ctacagtgga 1020
cgtggtggcc ctaggccgct gcagcctgcg cacactaagc cgctgcagcc tgcactcggc 1080
cagcccagcc tcagtccgct cgctggggcg cttctcctcc gtctccagcc cacagccccg 1140
cagccgccac ccatcctcct ccagtgacac ctggagccac tctcaatcct ccgacaccat 1200
tgtgtctgac ggttccaccc tctcctctaa gggtggctct gagggccagc cggagagctc 1260
tacggctagc aatagcgtgg taccccctcc ccagggaggc agtgggaggg gctctcccag 1320
tgggggcagc actgctgagg cctcagacac actcagcatt cggagcagtg ggcagttgtc 1380
tggccggagt gtgtccctgc gtaagctgaa gcggcctcca ccccctcccc gccggaccca 1440
ctccctccat cagcggggct tagcagtgcc tgatgggcca ttagggttgc cccctaagcc 1500
tgagcgtaag cagcagcccc agctgcctcg gccacccacc actggtggct cagaaggggc 1560
gggggcagca ccctgtccac ccaacccagc caacagctgg gtacctggct tgtctccggg 1620
tggttcccgg cgccccccac ggtccccaga acggacactt tcgccctcca gtggatactc 1680
gagccaaagt ggtactccca ccctccctcc caagggcctg gcaggtcccc ctgcttcccc 1740
aggcaaggcc cagcccccta aaccagagcg tgtcacgtct cttcgctccc ctggggcctc 1800
cgtctcctct tccctcacgt ctttatgttc ctcctcctct gacccagccc cctcagaccg 1860
ctctgggcca cagatattga cccccctggg tgacaggttt gtcatacctc ctcaccccaa 1920
ggtgcctgcc cccttctccc cacctccctc caagcccagg agccctaacc cagctgcccc 1980
tgctctagcc gcccctgctg tggttcctgg gcctgtttct accactgacg ccagtcctca 2040
gtcccctccc actccccaga caaccttgac tccactgcag gagtctcctg tcatctccaa 2100
agaccagtca cccccacctt ccccaccccc atcttatcat ccacccccac cacccactaa 2160
gaagccagag gtggttgtgg aggcaccatc tgcctcagag actgctgagg agcccctcca 2220
agatcccaac tggccccctc ccccaccccc tgcccctgag gagcaggacc tgtccatggc 2280
tgacttcccc ccaccagagg aggctttttt ctctgtggcc agccctgagc ctgcaggccc 2340
ttcaggctcc ccagagcttg tcagctcccc ggctgcttcg tcctcctcag ctactgcttt 2400
gcagattcag cccccgggta gcccagaccc tcctccagct ccgccagccc cagctcctgc 2460
tagttccgcc ccagggcatg tggccaagct ccctcagaag gaaccggtgg gctgtagcaa 2520
gggtggtggg cctcccaggg aggacgtagg tgcgcccctg gtcacgccct cgctcctgca 2580
gatggtgcgg ctgcgctccg tgggtgctcc aggaggggct cccaccccag cactggggcc 2640
atcggccccc cagaaaccac tgcgaagggc cctgtcaggg cgggccagcc cagtgcctgc 2700
cccctcctca gggctccatg ctgcggtccg actcaaggcc tgcagcctgg ccgccagtga 2760
aggcctctca agtgctcagc ccaacggacc gcctgaggca gagccacggc ctccccagtc 2820
ccctgcctca acggccagtt tcatcttctc caagggctct aggaagctgc agctggagcg 2880
gcccgtgtcc cctgagaccc aggctgacct ccagcggaat ctggtggcag aactccggag 2940
catctcagag cagcggccac cccaggcccc aaagaagtca cctaaggctc ccccacctgt 3000
ggcccgcaag ccgtctgtgg gagtcccccc acccgcctcc cccagttacc ctcgagctga 3060
gccccttact gctcctccca ccaatgggct ccctcacacc caggacagga ctaagaggga 3120
gctggcggag aatggaggtg tcctgcagct ggtgggccca gaggagaaga tgggcctccc 3180
gggctcagac tcacagaaag agctggcctg accaccaggc acctcactgg cactgctgac 3240
ccatcccaga aacacaatct cagggacccg agcagctcca aggacgagag gatacagcag 3300
acacaaccta atagagaggg cgcctgcagc cttaacctcc acggccttcg atacttatgc 3360
aagcctggtg ttgctcctgt cctcagagtc atcctgcgct catgcctttt cccgaatggg 3420
ttcacctctg gcagttgccg cttcagtctt ggccttagcc tcatcttgaa gtgggtagct 3480
ggcgggagag ggtggctgcg ccccctgctg gccctgaggc tgcagagttg ggagcaggac 3540
acctcacctg agtttcattt tttttcatgt ccaaaccatg cacatactat agtccagaat 3600
caaagcactt ttgaaaagtg gctgcatggc catcctccag ggcccaggaa gttgcattcc 3660
aagggcctgt ttacatggca gcagaatcca tccccggcag tcagcccata gcttgggacc 3720
agtctgtgcc ctcctgccca gtccagttta ctcctcttgg ttcctgaagg tggccaagtc 3780
attgtgttcc cacaggcttc tctaggctgg gggcaggtgt ggggctgtgg aattccaaag 3840
cacaaaaggt gcagagggga ttggccttcc tgtgcctcaa ctcaccaacc accctcctgc 3900
cttccagttc tgccaggtgc tccatgctgg ggacaagtag gagactgcca gggcccaaag 3960
aaatgggtga gcagtagagt catctcgggg cacttggcag tgtcaagcac ctgccccttg 4020
cctccttgac cacactgggg tgggtgggcc cccagcactt cagaggcagg agcctttggg 4080
ctgagcaagc actgaggagg tggatggaag ggagcatctg gaggggggga gcttccttga 4140
gcagtgggcc caggcctggc cctccacact tcattctctg acctttctct ctcctcattt 4200
cggtgcatgt cctttctgca gctgcctttc agcacaggtg gttccactgg gggcagctaa 4260
cgctgagtga caaggatggg aagccacagg tgcattttac tcaagtcttc tctagtcaat 4320
gaggggcacc cagtgcttct agggcaggct gggtggtggt cccctaggta tcagcctctc 4380
ttactgtact ctccgggaat gttaaccttt ctattttcag cctgtgccac ctgtctaggc 4440
aagctggctt ccccattggc ccctgtgggt ccacagcagc gtggctgccc cccagggcca 4500
ccgcttcttt cttgatcctc tttccttaac agtgacttgg gcttgagtct ggcaaggaac 4560
cttgctttta gcttcaccac caaggagaga ggttgacatg acctccccgc cccctcacca 4620
aggctgggaa cagaggggat gtggtgagag ccaggttcct ctggccctct ccagggtgtt 4680
ttccactagt cactactgtc ttctccttgt agctaatcaa tcaatattct tcccttgcct 4740
gtgggcagtg gagagtgctg ctgggtgtac gctgcacctg cccactgagt tggggaaaga 4800
ggataatcag tgagcactgt tctgctcaga gctcctgatc taccccaccc cctaggatcc 4860
aggactgggt caaagctgca tgaaaccagg ccctggcagc aacctgggaa tggctggagg 4920
tgggagagaa cctgacttct ctttccctct ccctcctcca acattactgg aactctatcc 4980
tgttaggatc ttctgagctt gtttccctgc tgggtgggac agaggacaaa ggagaaggga 5040
gggtctagaa gaggcagccc ttctttgtcc tctggggtaa atgagcttga cctagagtaa 5100
atggagagac caaaagcctc tgatttttaa tttccataaa atgttagaag tatatatata 5160
catatatata tttctttaaa tttttgagtc tttgatatgt ctaaaaatcc attccctctg 5220
ccctgaagcc tgagtgagac acatgaagaa aactgtgttt catttaaaga tgttaattaa 5280
atgattgaaa cttg 5294
<210>15
<211>988
<212>DNA
<213>人
<400>15
gtcgtgaggc gggccttcgg gctggctcgc cgtcggctgc cggggggttg gcctgggtgt 60
cattggctct gggaagcggc agcagaggca gggaccactc ggggtctggt gtcggcacag 120
ccatggcggg cgcgttggtg cggaaagcgg cggactatgt ccgaagcaag gatttccggg 180
actacctcat gagtacgcac ttctggggcc cagtagccaa ctggggtctt cccattgctg 240
ccatcaatga tatgaaaaag tctccagaga ttatcagtgg gcggatgaca tttgccctct 300
gttgctattc tttgacattc atgagatttg cctacaaggt acagcctcgg aactggcttc 360
tgtttgcatg ccacgcaaca aatgaagtag cccagctcat ccagggaggg cggcttatca 420
aacacgagat gactaaaacg gcatctgcat aacaatggga aaaggaagaa caaggtcttg 480
aagggacagc attgccagct gctgctgagt cacagatttc attataaata gcctccctaa 540
ggaaaataca ctgaatgcta tttttactaa ccattctatt tttatagaaa tagctgagag 600
tttctaaacc aactctctgc tgccttacaa gtattaaata ttttacttct ttccataaag 660
agtagctcaa aatatgcaat taatttaata atttctgatg atgttttatc tgcagtaata 720
tgtatatcat ctattagaat ttacttaatg aaaaactgaa gagaacaaaa tttgtaacca 780
ctagcactta agtactcctg attcttaaca ttgtctttaa tgaccacaag acaaccaaca 840
gctggccacg tacttaaaat tttgtcccca ctgtttaaaa atgttacctg tgtatttcca 900
tgcagtgtat atattgagat gctgtaactt aatggcaata aatgatttaa atatttgtta 960
aaaaaaaaaa aaaaaaaaaa aaaaaaaa 988
<210>16
<211>4908
<212>DNA
<213>人
<400>16
ggataacctc gcagggtggg ccggagggcg ggcgccgccg ctgcctgtgc tgcggcgatg 60
gcccagtgtg tacaatcagt gcaggagcta atcccggact ccttcgtccc ctgtgtcgct 120
gcgctgtgca gcgacgaagc cgagcggctc actcgtctca atcacctcag cttcgcggag 180
ctgcttaagc ccttctcccg cctcacttcc gaggttcaca tgagagatcc taataatcaa 240
cttcacgtaa ttaaaaattt gaagatagca gtaagcaaca ttgtcaccca gccacctcag 300
cctggagcca tccggaagct tttgaatgat gttgtttctg gcagtcagcc tgcagaagga 360
ttagtagcta atgtgattac agcaggagat tatgacctta acatcagtgc cactactcca 420
tggtttgagt cttacagaga aacctttctt cagtcgatgc cagcatcgga tcatgaattt 480
ctgaaccact atttagcatg tatgttggta gcgtcatcta gtgaagctga acctgtggaa 540
cagttttcaa agttgtcaca agaacagcat cgaattcagc acaacagtga ttattcctac 600
cccaagtggt ttataccaaa tacacttaaa tactatgtac ttttacatga tgtaagtgca 660
ggagatgaac agagagctga atcaatttat gaagaaatga aacagaaata tggaactcag 720
ggttgctatt tacttaaaat taattctcga acatctaatc gagcatcaga tgaacagata 780
ccagatcctt ggagtcagta tctccagaaa aatagtattc aaaaccagga atcatatgaa 840
gatggccctt gtactataac ttcaaataag aattctgata ataacttgct ttcattggat 900
ggattagata acgaagtcaa agatggctta ccaaataact ttagagctca cccacttcag 960
ttggagcaat ccagtgaccc ttctaacagt attgatggcc cagatcatct aagatctgct 1020
tcatcgttac atgaaacaaa gaaaggaaat actggaataa ttcatggtgc atgtttaaca 1080
cttactgatc atgatagaat tcgacagttt atacaagagt tcacatttcg gggccttttg 1140
ccacatatag agaaaacaat taggcaatta aacgatcagc taatatcaag aaaaggtttg 1200
agtcgatctc tattttctgc aactaaaaaa tggtttagtg gcagtaaagt tccagaaaag 1260
agcattaatg acctgaaaaa tacatctggc ttgctgtatc ctccggaagc accagaactt 1320
caaatcagga aaatggctga cttatgtttt ttggtgcagc attatgattt ggcttacagt 1380
tgctatcata ctgcaaagaa agattttctt aatgatcaag caatgcttta tgcagctggt 1440
gccttggaaa tggcagcagt gtctgctttt cttcaaccag gagcacctag gccatatcct 1500
gctcattaca tggatacagc aattcagaca tacagagata tctgcaagaa tatggtgttg 1560
gctgaaagat gtgtgttgct tagtgctgaa cttttaaaaa gccaaagcaa atattcagag 1620
gctgcagctc tcctaatacg gttgaccagt gaggattctg atcttcgaag tgcacttctt 1680
ttggaacagg cagcacattg ctttataaac atgaaaagtc ccatggttag aaaatatgca 1740
tttcatatga tattggcagg ccatcgattt agtaaagcag ggcagaaaaa gcatgcttta 1800
cgctgttatt gtcaagccat gcaagtttac aaaggaaaag gctggtctct tgcagaggat 1860
cacattaatt tcactattgg gcgccagtcc tatactctta gacagctgga taatgctgtg 1920
tctgctttta ggcatattct aattaatgaa agtaaacaat ctgctgctca acagggggct 1980
ttcctcagag aatatcttta tgtttacaag aatgtaagtc agctgtcacc agatggtcct 2040
ttgccacagc ttcctttacc gtatattaac agttcagcaa cacgggtttt ttttggccat 2100
gacagacgac cagcggatgg tgaaaaacaa gcagctactc atgtaagtct tgatcaagaa 2160
tatgattctg aatcctctca gcagtggcga gaacttgagg aacaagttgt ttctgtggtt 2220
aacaaaggag taattccatc caattttcat cccacacaat actgtttgaa cagttactca 2280
gataattcaa gatttccact tgcagttgta gaagaaccaa ttacagtgga agtggctttt 2340
agaaaccctt tgaaagttct acttttgttg actgatttgt cattgctttg gaagtttcat 2400
cctaaagatt tcagtggaaa ggataatgaa gaagttaaac aactagttac aagtgaacct 2460
gaaatgattg gagctgaagt tatttcagag ttcttaatta atggcgaaga atcaaaagtg 2520
gcaagactaa agctctttcc ccatcacata ggggagctgc atattctggg agttgtttat 2580
aatcttggca ctattcaggg ctctatgaca gtagatggca ttggtgctct tcccggatgt 2640
cacacaggaa aatattcctt gagtatgtca gtccgaggga agcaggattt agaaattcaa 2700
ggtcctcgac ttaacaacac aaaagaagag aaaacatctg ttaaatatgg ccctgatcga 2760
cgtttagatc ccataatcac agaagaaatg ccactgttgg aggtgttctt tatacatttt 2820
cctacagggc ttctctgtgg agaaatccga aaagcatatg tagaatttgt caatgtcagc 2880
aaatgtccac ttactggatt gaaggttgtt tctaaacgtc cagagttctt tactttcggt 2940
ggtaatactg ctgttctaac accactaagt ccctcagctt ctgagaattg tagtgcttac 3000
aagactgttg tgacagatgc tacctctgtg tgtacagcac tcatatcatc agcttcttct 3060
gtagactttg gcattggcac aggaagtcaa ccagaggtga ttcctgttcc ccttcctgac 3120
actgttcttc tacccggagc ctcagtgcag ctgccaatgt ggttacgtgg gcctgatgaa 3180
gaaggtgtcc atgaaattaa ctttttgttt tactatgaaa gtgtcaaaaa gcagccaaaa 3240
atacggcaca gaatattaag acacactgca attatttgta ccagtcggtc tttaaatgta 3300
cgggccactg tctgcagaag taattctctt gaaaatgaag aaggcagagg aggcaatatg 3360
ctagtctttg tggatgtgga aaataccaat actagtgaag caggcgttaa ggaattccac 3420
atagtgcaag tatcaagtag tagcaaacac tggaagttac agaaatctgt aaatctttct 3480
gaaaacaaag atgccaaact tgccagtagg gagaagggaa agttttgctt taaggcaata 3540
agatgtgaga aagaagaagc ggccacacag tcctctgaaa aatatacctt tgcagatatc 3600
atctttggaa atgaacagat aataagttca gcaagcccat gtgcagactt cttttatcga 3660
agtttatctt ctgaattgaa aaaaccacaa gctcacttgc ctgtgcatac agaaaaacag 3720
tcaacagagg atgctgtgag attgattcaa aaatgcagtg aggtagattt gaatattgtc 3780
atattatgga aggcatacgt tgtggaagac agtaaacagc ttattttgga aggtcaacat 3840
catgttattc ttcgcactat aggaaaagaa gccttttcat atcctcagaa acaggagcca 3900
ccagaaatgg aactattgaa atttttcagg ccagaaaaca ttacagtttc ctcaaggcca 3960
tcagtagagc agctttctag tctcattaaa acgagtcttc actacccaga atcatttaat 4020
catccatttc atcaaaaaag cctttgttta gtaccagtca ctcttttact ttccaattgt 4080
tctaaggctg atgtagatgt catagttgat cttcggcata aaacaacaag tccagaagca 4140
ctggaaatcc atggatcatt cacatggctt ggacaaacac agtataaact tcaacttaaa 4200
agccaggaga ttcacagtct gcagctgaaa gcatgctttg ttcatacagg tgtttataac 4260
cttggaactc ctagggtatt tgccaagtta tcggaccaag ttacagtgtt tgaaacaagt 4320
cagcagaatt ccatgcctgc cctgatcatc atcagtaatg tgtgacaact tggaaatttg 4380
tactgaaatc cacaataatc agtttttgct ggatgggttt tacagcagta tttgatatac 4440
ctaacttgtt atggaggttg attgatatct gatccctgca aaatactttg acttgtcatt 4500
ttgttgatga tgcaaagcac gttggactga gaatacttaa cattcttttt ctgtatttct 4560
ttaaaccctg agaataattt acatgctcat aatacaggat atcagcatat ttgtgcacct 4620
tattaagccc catcttaaga aaacacaaag tctaagtctg ctgttacaac ttgtcaatgg 4680
tatacgaata ttaggagatg attctgagaa aggaaaggcc ttgttggcag tactcctgtt 4740
aagccattag tctctaaatt ccagctttac tgtgaagttc tatagagtgt taaatacaaa 4800
ttttcctgtc ttgcttcaca cagttcctta aaatcagttt tgaactttgg tcatagagtc 4860
ttcatatttc agtatttggt ggtccctatg acttatacat aactttgt 4908
<210>17
<211>435
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(30)..(30)
<223>任意碱基
<220>
<221>misc_特征
<222>(49)..(49)
<223>任意碱基
<220>
<221>misc_特征
<222>(75)..(75)
<223>任意碱基
<220>
<221>misc_特征
<222>(76)..(76)
<223>任意碱基
<220>
<221>misc_特征
<222>(78)..(78)
<223>任意碱基
<220>
<221>misc_特征
<222>(79)..(79)
<223>任意碱基
<220>
<221>misc_特征
<222>(109)..(109)
<223>任意碱基
<220>
<221>misc_特征
<222>(136)..(136)
<223>任意碱基
<220>
<221>misc_特征
<222>(137)..(137)
<223>任意碱基
<220>
<221>miac_特征
<222>(149)..(149)
<223>任意碱基
<220>
<221>misc_特征
<222>(227)..(227)
<223>任意碱基
<220>
<221>misc_特征
<222>(236)..(236)
<223>任意碱基
<220>
<221>misc_特征
<223>任意碱基
<220>
<221>misc_特征
<222>(342)..(342)
<223>任意碱基
<220>
<221>misc_特征
<222>(363)..(363)
<223>任意碱基
<220>
<221>miac_特征
<222>(389)..(389)
<223>任意碱基
<220>
<221>misc_特征
<223>任意碱基
<400>17
ggtagaaatg attgtgatgt acaaattttn tattttgatc atacttaana agacagagca 60
gactcacatt cattnncnna atagtatcac tgtacacata gcgaatttnt ggcgctttta 120
gattgctctg aaaatnnctg aagagttgnc catagcagcc tggtaagcct tttcctttcc 180
cccaaagctc tcctgccctt tgcagaaaga ctgttggtga caactgntgc taactnaata 240
gcatgnggtt gaacttcgcc aaaatccttc cacctcctcc catagggcaa caggggtgac 300
ttgggcttaa agggcattga gtaagcaagt aggttatcag anaacagagg gaagattcca 360
ttntagataa tttccaaata ttacaattng tggaactcag agttcaactg ctcagttcct 420
tcttcngctg accct 435
<210>18
<211>2224
<212>DNA
<213>人
ctttagatct gtgcagcctt tgcgtgccaa acttgtgaaa ttccttttac cttttttgga 60
gtacttgcta taaagccacc tgtcaacaaa cccccattat gtacagaata ggacctatcc 120
agtagccagg ccagtaggca gttggggaag gtgggaagga tccagcgagg cccctgagcc 180
tgcacctgga caggtgtacg tctgcaccca tcaccctcag caccaggcca ccctgcagtc 240
cacttactgt actgtgttgt ggaaggatat gctaagtgat gaaagttgcg agcagtctca 300
ctggtcgtgt aaactttttt ttttttttgg aaattgaagc tgtagagtgc tgcccgaaat 360
ctctaggaag ttggtggcaa gggacagcac tcacactctt ctggtcatga tctctgatct 420
ccacctcaaa tgacaataaa aaactggtcc aacgaagaca ctgctcagca cttcagccat 480
caggactaat ccatcgatga ctggaaaaga ggctagcttt gaggaaaaca gcctgggctc 540
ttgggagcag agtccagtgg gtgtgaggct gacttgccga cggtcggcag gtaatggctc 600
tcagccggcg aggcggtccc acagctctcc tcccagggca gcctgaggag gaggaggccg 660
ggtgcctgtt tggtggcagc ttcagcctag ggatacctga agctgttgag caacaccttt 720
atgaaatgtt gccagagcag caacacttcc ctgtgggcac agccccggga aatccggtac 780
caagtgagca aggtggcagg acccacccaa gcctgatacg catctgggcc cgccgggctc 840
agcaggggag gctgctacgg ctgcccactt cccagcaccg tctgtcaggc ttgaacccct 900
ctgtgctgtt cccttcctgg ctaataggga gacccttcgc aggcacccac tgtttcaact 960
tgaccctccc accccctgct actctcctcc acacacccct ccgttccgct agcctaccct 1020
gtcagccttt caataaaagt tatgcacaaa tgtgaacacc tgagatggag ctgaacattt 1080
cttcactttg ttctttttct gaagtcaaac tcttatcaaa tgccctaaaa ttattaccac 1140
ccaagagaaa caggaaaaag gttacatgtt tttgtttact gagagtaaga tcacctgcat 1200
ctggaagacg ggctggtaaa ttggtttggc tacagaacag aaagaaaaca aaaacaaacc 1260
tcgtaaggga agtatcgcac tcagacacca ccacttccta gagccaaatg agcaatccca 1320
aactgcaagt gccgtaagtg ggcctgtgac gtcacaccgc ccggcccgag gtatcgcatg 1380
tgcgggggag gcccacacta cagctgtcct ctcgtctaga aggcaccacc tcgctttcat 1440
gtcccgtgtg ttttggaaaa gcagtatggt gtgtcatgtc tagcggcgaa cacttccctc 1500
cctctgtcct tgaggttgta atataaaaac tgtgtttctg tacgtgtggg tgggaattct 1560
ctgacggtgc tcgttcatag cacaagctta cgctgagttc tgaactgtcg ttcacagctg 1620
cgtgtctgca tggtgtcgca tctgttgtac ctttggggaa aatttgtatg taaatgtaca 1680
gaaataaaaa cgttgcccca ttaacagatt tcctctggaa tgtcttccct acctcacctg 1740
atggtatcca ccgaagggca tttcactacc attaatggtg agtaataaaa tcctccgtgt 1800
tcattcagac ctcactgcgt cactactttg aacgcctctg taagctgtgt cttcacccgc 1860
cccgaggtgg gtggagggag gcctctcact ctgcttcgag tcctggtctt aaaggtagtc 1920
agaggcagag gctggattaa acacacactg tttaccaagt gccactctca gaccacctga 1980
gagacggggg gccatcagta aaattaagag gaattttttt cccttgttcg tgtatgttct 2040
gctgatccgt ggcctgaagg ttcctagaga cgtcaagaaa tgaatatctt acactgtgat 2100
tctgtgagga aagactggta acccaaaact ctcttctcta atgtattttt taacgaaaat 2160
gacaatattt ctttaataaa gtatttatac caaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2220
aaaa 2224
<210>19
<211>2244
<212>DNA
<213>人
<400>19
gttgtttaaa agcaaggcat gcttgtggat gactctgtaa cagactaatt ggaattgttg 60
aagctgctcc ctggttccac tctggagagt aatctgggac atcttagtgt tttgttttgt 120
ttttttccct cctctttttt tgggggggag tgtgtgtggg gtttgttttt tagtcttgtt 180
tttttaattc attaaccagt ggttagcctt aaggggagga ggacggattg attccacatt 240
ccacttccta gatctagttt agaaaacatg ttccccatct ggtgctctta ggaaggagta 300
tagtaaatgc ctcatttaat aacatactcc tttttgaaag ttgccttttc tctccaccct 360
tgagtagatc cagtatttga tgaaactcat gaaagtgggt ggagcccatc ttccccctcc 420
tcttttctag gacgcactat atgtgactgt gactttaagg acatttgttt gccatttgct 480
gatttttttg ggaagttaat ttctaacttc tttcactgat aaatgaagaa aagtattgca 540
cctttgaaat gcaccaaatg aattgagttt gtaattaaaa aaattttttt tccctttcag 600
tcattgtctt atatgcttag catagatttg cagctcagta gtatatgtgt tcctagaatg 660
cagctgaaga cctgttatgt agaggaaata cgaggggtgg tgctagaaga cagacatctg 720
tggaatgatt cacatcctct caagttagga ggatggaggc ctgcttcatt aagaagctgg 780
gggtagggtg ggggtgggga gaacacttaa caacatgggg accagtcagg ggaatcccct 840
tatttctgtt ttgcatatga ggaaccctag agcagccagg tgaggctctc tagtttaata 900
aaaatcatgg aaagactctt aatgcagact cttcttaagt gttaataggg attttttcag 960
cttattttgg ttgcagtttc caatttttaa aaatgttgag gtaatctttc ccaccttccc 1020
aaacctaatt cttgtagatg cattagtgtt gaaccaatgc ttctcatgtc tcaatcttgt 1080
atatcatctt ttcagatgta ttaacaaaca aaaccttaaa aagagtagat gaattgccaa 1140
acacaattcc taccaataat aaatcgatca actctatcta ttcaggaaag caggaagcat 1200
ttggaccaca gtgcatgaaa acttcaacat tctgttatta gataatgaat caaccaaatg 1260
aacaatccag agaaaagaaa attgcaataa taaaaggtaa attaacagaa agataatata 1320
agcaagatag taatagttga ccattctgaa aagcttataa catcactcat catccagcat 1380
cctttctgaa aacaaaggat ttttaaatca ctttatgcac atatacaaca taggaggttg 1440
gcaaaataat gcactatttc ttaacagcca tgtctcttgt agaacttcaa gttaatctac 1500
aaatgaccat tgtgtcttaa tttagattat gaataccaca ttagtcaggt atttgcacta 1560
acccttaata gtatatacag tttctatgga aaattcagtg gtccaaaaat ttccgtagaa 1620
tttgagagga cgttggtggg ctgaagatag ctccttgagg gtcactgatg taggctgcaa 1680
tgggggttca caaggccctg acaccgtatt tatagtctaa cctttttatg aaaatctgac 1740
tacagctatt taaggagtag tcttaatagc tgaaaatgaa gatagagaaa gacaccaaga 1800
atatgacaca gtttacattc tagtgaggga cacaacaaaa tcaaatttaa aaaagagtgt 1860
aatagatgct gataaatact gtagataaag cacataagaa aatagaaata aaggctgtca 1920
atggagaagt catgattttt attttattta tttatttatt tatttgagac agagtcaggc 1980
tctgtgcagg ctggagtgca atggtgtgat ctcgctcact acaacctctg ctcctggctc 2040
aagctatcct cccacctcag ctctcaagta gctgggatca caggtgcgtg ctaccatgcc 2100
cggctaattt tttgtagaga tgaggttttg ccatgttgcc caggctggtc tcgaactcct 2160
ggactcaact gaccccacct cggcctctca aagtgctgag attataggcg tgcagccggc 2220
agctggccat tgtttatgtt ctgc 2244
<210>20
<211>351
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(62)..(62)
<223>任意碱基
<220>
<221>misc_特征
<222>(121)..(121)
<223>任意碱基
<220>
<221>misc_特征
<222>(207)..(207)
<223>任意碱基
<220>
<221>misc_特征
<222>(220)..(220)
<223>任意碱基
<221>misc_特征
<222>(276)..(276)
<223>任意碱基
<220>
<221>misc_特征
<222>(300)..(300)
<223>任意碱基
<220>
<221>misc_特征
<222>(315)..(315)
<223>任意碱基
<220>
<221>misc_特征
<222>(336)..(336)
<223>任意碱基
<400>20
tctacttcca catcggcgag accgagaagc gctgtttcat cgaggaaatc cccgacgaga 60
cnatggtcat cggcaactat cgtacccaga tgtgggataa gcagaaggag gtcttcctgc 120
nctcgacccc tggcctgggc atgcacgtgg aagtgaagga ccccgacggc aaggtggtgc 180
tgtcctggca gtacggctcg gagggcnctt tcacgttcan ctcccacacg cccggtgacc 240
atcaaatctg tctgcactcc aattcttacc aggatngctc tctttcgctg gtgggcaaan 300
tgcgtgttgc atctngacat ccaggtttgg gggagnatgc caacaaatta c 351
<210>21
<211>2631
<212>DNA
<213>人
<400>21
accttccaac ccagccctcg gctgagccgc gccgcaccat gcccgccgtg gacaagctcc 60
tgctagagga ggcgttgcag gacagccccc agactcgctc tttactgagc gtgtttgaag 120
aagatgctgg caccctcaca gactatacca accagctgct ccaggcaatg cagcgcgtct 180
atggagccca gaatgagatg tgcctggcca cacaacagct ttctaagcaa ctgctggcat 240
atgaaaaaca gaactttgct cttggcaaag gtgatgaaga agtaatttca acactccact 300
atttttccaa agtggtggat gagcttaatc ttctccatac agagctggct aaacagttgg 360
cagacacaat ggttctacct atcatacaat tccgagaaaa ggatctcaca gaagtaagca 420
ctttaaagga tctatttgga ctcgctagca atgagcatga cctctcaatg gcaaaataca 480
gcaggctgcc taagaaaaag gagaatgaga aggtgaagac cgaagtcgga aaagaggtgg 540
ccgcggcccg gcggaagcag cacctctcct cccttcagta ctactgtgcc ctcaacgcgc 600
tgcagtacag aaagcaaatg gccatgatgg agcccatgat aggctttgcc catggacaga 660
ttaacttttt taagaaggga gcagagatgt tttccaaacg tatggacagc tttttatcct 720
ccgttgcaga catggttcaa agcattcagg tagaactgga accgaggcgg aaaagatgcg 780
ggtgtcccag caagaattac tttctgttga tgaatctgtt tacactccag actctgatgt 840
ggccgcacca cagatcaaca ggaacctcat ccagaaggct ggttacctta atcttagaaa 900
caaaacaggg ctggtcaccg ccacctggga gaggctttat ttcttcaccc aaggcgggaa 960
tctcatgtgt cagcccaggg gagccgtggc tggaggtttg atccaggacc tggacaactg 1020
ctcagtgatg gccgtggatt gcgaagaccg gcgctactgc tttcagatca ccacgcccaa 1080
tggaaaatcg ggaataatcc tccaggctga gagcagaaag gaaaatgaag agtggatatg 1140
tgcaataaac aacatctcca gacagatcta cctgaccgac aaccctgagg cagtcgcgat 1200
caagttgaat cagaccgctc tgcaagcagt gactcctatt acaagttttg gaaaaaaaca 1260
agaaagctca tgccccagcc agaacctgaa aaattcagag atggaaaatg aaaatgacaa 1320
gattgttccc aaagcaacag ccagtctacc tgaagcagag gagctgatcg cgcctggagc 1380
gccgattcaa ttcgatattg tgcttcctgc tacagaattc cttgatcaga acagagggag 1440
caggcgtacc aacccttttg gtgaaactga ggatgaatca tttccagaag cagaagattc 1500
tcttttgcag cagatgttta tagttcggtt tttgggatca atggcagtta aaacagacag 1560
cactactgaa gtgatttatg aagcgatgag acaagtattg gctgctcggg ctattcataa 1620
catcttccgc atgacagaat cccatctgat ggtcaccagc caatctttga ggttgataga 1680
tccacagact caagtatcaa gggccaattt tgaacttacc agtgtcacac aatttgctgc 1740
tcatcaagaa aacaagagac tggttggttt tgtcatccgt gttcctgaat ccactggaga 1800
agaatctctg agtacataca tttttgaaag caactcagaa ggcgaaaaga tatgttatgc 1860
tattaatttg ggaaaagaaa ttattgaggt tcagaaggat ccagaagcac tggctcaatt 1920
aatgctgtcc ataccactaa ccaatgatgg aaaatatgta ctgttaaacg atcaaccaga 1980
tgacgatgat ggaaatccaa atgaacatag aggcgcagaa tccgaagcat aactcacttg 2040
cgcctgtggg ggaagagcga acaggaagga gagctacctc ctaagggttt taacgtctct 2100
gacatacagg cacactgacc tgatttccga aggctgacaa tcgtttgtgg aatgtaatct 2160
tgatgccttg atactgagac ttgggaggga aactaagaaa tggttgacag cgttcccacc 2220
catctacaat gttattttag gtgctttgtg gtaagtcttt tttcttagat tgcgctaaaa 2280
tttcttagat tgttcagcgc tcagaacaaa agtttgaaaa atgcattgtt catatgaatg 2340
tcatctcttt tcagtttcca gtatcctttt taaaaaatgg caaaagccta gatttacaat 2400
ttgatgaaca ctaaatattt cttattaata taatctattt ttgtatttta cttaatgagc 2460
tttaagtgcc tgtcgttctg aaaattgtgt atttataatt cagcttatct cataattgga 2520
cctaatagca tttctttgtg cagttaggtg atgagcactg ctttgaggcc caagcactag 2580
tagagatgcg cgatacaggt ctagtttcgg taactgttcc agacatcaag c 2631
<210>22
<211>2851
<212>DNA
<213>人
<400>22
agcatctcag gccatcatcc tgaaacttgg cagccttcgt ggagtataag gacagcatta 60
ttagccatca ttgggtttac tgccaacaaa aggagaggga gccataggtt ctctagatta 120
cactcctgag gaaagaagag cacttgccaa aaaatcacaa gatttctgtt gtgaaggatg 180
tggctctgcc atgaaggatg tcctgttgcc tttaaaatct ggaagcgatt caagccaagc 240
tgaccaagaa gccaaagaac tggctaggca aataagcttt aaggcagaag tcaattcatc 300
tggaaagact atctctgagt cagacttaaa ccactctttt tcactaactg atttacaaga 360
tgatatacct acaacattcc agggtgctac ggccagtaca tcgtacggac tccagaattc 420
ctcagcagca tcctttcatc aacctaccca acctgtagct aagaatacct ccatgagccc 480
tcgacagcgc cgggcccagc agcagagtca gagaaggttg tctacttcac cagatgtaat 540
ccagggccac cagccaagag acaaccacac tgatcatggt gggtcagctg tactgattgt 600
catcctgact ttggcattgg cagctcttat attccgacga atatatctgg caaacgaata 660
catatttgac tttgagttat aatatggttt tgtgacttat gagctgtgac tcaactgctt 720
cattaaacat tctgcattgg gtataatcta agaattgttt acaaaaagat tattttgtat 780
ttacccttca ttcctttttt tgatccttgt aagtttagta taaatatatc tagacattca 840
gactgtgtct agcagttacg tcctgcttaa agggactaga agtcaaagtt ccttgtctca 900
ctatttgatc tgctttgcag ggaaataact tgttttttct catgtttcat cttcttttta 960
tgtaaatttg taatactttc ctatattgcc ctttgaaatt tttggataaa agatgatgtt 1020
ttaagttcca atgagtatta ctagttactc aataccactt attgagtact ctgtttctac 1080
gtatgtagaa tgtataggga tagaagagtt gaaaagggaa agcaaaactt cttaagtggc 1140
ttccttaaaa tgtcattcat aggagatgta ctggaattgc tcattctgtg actttatttg 1200
tgtcctaaac attcttcagt gaaaataatt ttatttcagt caaacattta tgaggaaatg 1260
agatcacatc tttgtcactg gatgctactt gaagagggag tactttgtaa ccactttgat 1320
atgctgttat caccaccccc tgccctctgc tgccataatc acacaaattt aaaaagaaag 1380
aaaacagtct tccatagatt tttaaggaag aaagggccca agtcaggaga tcgcttggtt 1440
ttcttccaga agttaaatgg ggggatctga agatttgaat gttcggtctg ctttgaaatg 1500
tatgtctttt gggaatgtat tatatgccta gctttataat caggtataaa attttaatta 1560
ttcccaggaa tatgcataat attgaatatt tcatgtccta ttttaataga aaacctcagg 1620
gcccaagtaa ccagtgatag aagttagaaa aaccccttta cttagaattg tccacctagt 1680
cagagcccaa gaaagaattt tcagtggaaa aatcaatata taacttagtg ctagctagcg 1740
ccacagactc tagtagataa tattatcatc ataatggctg gtgaaaccat ataatcacag 1800
aaaaacattg ccttcagcat gttcagttcg cagcactgag ggcactcttg agggtgttgt 1860
taatgaagat ttaattttta aatacaggtg gttccaagct ttcaaatagg ttatgctcca 1920
aaagtgttat ttgtaagtta atttttttac aagtcaaaca atgttggaag tggtatttag 1980
gttctagatc ggtccacgaa agttagccca tatgtatatc ttgaatagta taggggaggg 2040
tattcataaa gtccttatgt ggttttaact aagtgaaatt atggacaaga gaaataattg 2100
taaaatcgtc ttaaaggcaa atttaatttt tacccctgtt tatgggacat tcgttctatt 2160
aactgtcaga cacaatttct gttttcatct gagagccagg tttcctttat ttctacatct 2220
aaaataagaa catattgtac actattatat aatacagaat tgtcttacac tttaataaat 2280
tcgcatttta aaggtgttta caggattatt ttttatatct gtagctgaat ttgttaaagt 2340
ctaaaaagct caaggacttt atgaagatct cattatatga ggaaaatcat aggttaccat 2400
tttataactc tattgccata agaaaataca ctctaaaatc ttgatttgaa acatattaga 2460
aaccttgatt cagtgctcag tggtctccta gtaagaagtc accgacggta gcgtcatatg 2520
agaagaaaga aatccccacc acctcaacct ctgctgagat tgtgtgctag gaacagcctt 2580
ccctccgttt cccctcagtc aaacttgagc cagcctctgg atcgatgtga tcttattgca 2640
tgtttccatg gggtgtacct atactttaag ccaatcctgc tgcattcact gctaagttaa 2700
ataaaaagcc aagaagaaaa aaaaaatttt gcactgtgca gatcctttgc tatctgactt 2760
gcatctcttc ccccacctgt cagctagcca cctgcttgtt tgtgttggga tattttttag 2820
cacctgaagc accatctgaa aggggcacca t 2851
<210>23
<211>3473
<212>DNA
<213>人
<400>23
aagagcagcg gcgaggcggc ggtggtggct gagtccgtgg tggcagaggc gaaggcgaca 60
gctctagggg ttggcaccgg ccccgagagg aggatgcggg tccggatagg gctgacgctg 120
ctgctgtgtg cggtgctgct gagcttggcc tcggcgtcct cggatgaaga aggcagccag 180
gatgaatcct tagattccaa gactactttg acatcagatg agtcagtaaa ggaccacact 240
actgcaggca gagtagttgc tggtcaaata tttcttgatt cagaagaatc tgaattagaa 300
tcctctattc aagaagagga agacagcctc aagagccaag agggggagag tgtcacagaa 360
gatatcagct ttctagagtc tccaaatcca gaaaacaagg actatgaaga gccaaagaaa 420
gtacggaaac cagctttgac cgccattgaa ggcacagcac atggggagcc ctgccacttc 480
ccttttcttt tcctagataa ggagtatgat gaatgtacat cagatgggag ggaagatggc 540
agactgtggt gtgctacaac ctatgactac aaagcagatg aaaagtgggg cttttgtgaa 600
actgaagaag aggctgctaa gagacggcag atgcaggaag cagaaatggt gtatcaaact 660
ggaatgaaaa tccttaatgg aagcaataag aaaagccaaa aaagagaagc atatcggtat 720
ctccaaaagg cagcaagcat gaaccatacc aaagccctgg agagagtgtc atatgctctt 780
ttatttggtg attacttgcc acagaatatc caggcagcga gagagatgtt tgagaagctg 840
actgaggaag gctctcccaa gggacagact gctcttggct ttctgtatgc ctctggactt 900
ggtgttaatt caagtcaggc aaaggctctt gtatattata catttggagc tcttgggggc 960
aatctaatag cccacatggt tttgggttac agatactggg ctggcatcgg cgtcctccag 1020
agttgtgaat ctgccctgac tcactatcgt cttgttgcca atcatgttgc tagtgatatc 1080
tcgctaacag gaggctcagt agtacagaga atacggctgc ctgatgaagt ggaaaatcca 1140
ggaatgaaca gtggaatgct agaagaagat ttgattcaat attaccagtt cctagctgaa 1200
aaaggtgatg tacaagcaca ggttggtctt ggacaactgc acctgcacgg agggcgtgga 1260
gtagaacaga atcatcagag agcatttgac tacttcaatt tagcagcaaa tgctggcaat 1320
tcacatgcca tggccttttt gggaaagatg tattcggaag gaagtgacat tgtacctcag 1380
agtaatgaga cagctctcca ctactttaag aaagctgctg acatgggcaa cccagttgga 1440
cagagtgggc ttggaatggc ctacctctat gggagaggag ttcaagttaa ttatgatcta 1500
gcccttaagt atttccagaa agctgctgaa caaggctggg tggatgggca gctacagctt 1560
ggttccatgt actataatgg cattggagtc aagagagatt ataaacaggc cttgaagtat 1620
tttaatttag cttctcaggg aggccatatc ttggctttct ataacctagc tcagatgcat 1680
gccagtggca ccggcgtgat gcgatcatgt cacactgcag tggagttgtt taagaatgta 1740
tgtgaacgag gccgttggtc tgaaaggctt atgactgcct ataacagcta taaagatggc 1800
gattacaatg ctgcagtgat ccagtacctc ctcctggctg aacagggcta tgaagtggca 1860
caaagcaatg cagcctttat tcttgatcag agagaagcaa gcattgtagg tgagaatgaa 1920
acttatccca gagctttgct acattggaac agggccgcct ctcaaggcta tactgtggct 1980
agaattaagc tcggagacta ccatttctat gggtttggca ccgatgtaga ttatgaaact 2040
gcatttattc attaccgtct ggcttctgag cagcaacaca gtgcacaagc tatgtttaat 2100
ctgggatata tgcatgagaa aggactgggc attaaacagg atattcacct tgcgaaacgt 2160
ttttatgaca tggcagctga agccagccca gatgcacaag ttccagtctt cctagccctc 2220
tgcaaattgg gcgtcgtcta tttcttgcag tacatacggg aaacaaacat tcgagatatg 2280
ttcacccaac ttgatatgga ccagcttttg ggacctgagt gggaccttta cctcatgacc 2340
atcattgcgc tgctgttggg aacagtcata gcttacaggc aaaggcagca ccaagacatg 2400
cctgcaccca ggcctccagg gccacggcca gctccacccc agcaggaggg gccaccagag 2460
cagcagccac cacagtaata ggcactgggt ccagccttga tcagtgacag cgaaggaagt 2520
tatctgctgg gaacacttgc atttgattta ggaccttgga tcagtggtca cctcccagaa 2580
gaggcacggc acaaggaagc attgaattcc taaagctgct tagaatctga tgcctttatt 2640
ttcagggata agtaactctt acctaaactg agctgaatgt ttgtttcagt gccatatgga 2700
ataacaactt tcagtggctt ttttttttct tttctggaaa catatgtgag acactcagag 2760
taatgtctac tgtatccagc tatctttctt ggatcctttt ggtcattatt tcagtgtgca 2820
taagttctta atgtcaacca tctttaaggt attgtgcatc gacactaaaa actgatcagt 2880
gtaaaaagga aaacccagtt gcaagtttaa acgtgttcga aagtctgaaa atagaacttg 2940
ccttttaagt taaaaaaaaa aaaagctatc ttgaaaatgt tttggaactg cgataactga 3000
gaaactctta ccagtccaca tgcaattaga catattcagc atatttgtta ttttaaaagg 3060
gagggttggg aggtttctta ttggtgattg tcacacggta taccatactc ctctccttca 3120
aagaatgaaa ggccttgtta aggagttttt tgtgagcttt acttctttgg aatggaatat 3180
acttatgcaa aaccttgtga actgactcct tgcactaacg cgagtttgcc ccacctactc 3240
tgtaatttgc ttgtttgttt tgaatataca gagccttgat ccagaagcca gaggatggac 3300
taagtgggag aaattagaaa acaaaacgaa ctctggttgg ggtactacga tcacagacac 3360
agacatactt ttcctaaagt tgaagcattt gttcccagga tttattttac tttgcatttc 3420
cttttgcaca aagaacacat caccatttcc ttttgcacaa agaacacatc acc 3473
<210>24
<211>401
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(252)..(252)
<223>任意碱基
<220>
<221>misc_特征
<222>(303)..(303)
<223>任意碱基
<220>
<220>misc_特征
<222>(390)..(390)
<223>任意碱基
<400>24
ttagattatt ttcaatttat tattcagaat aaatatatct tttttcttta acttctcaaa 60
tagttattga attgtattgg tttaaattaa atgcgtcatg tgtatatatc agtattaatt 120
caagagatac aaaaggaaat tgagtgaaaa ataagtctgc ctccttccca tcactctcat 180
gtctctacct agaggcaatt attgtcaaca gtttttgatg tgtctttcaa aaaatagtcc 240
attaagcctg gngtactaga tctcttttaa aagtttacaa cctgttacag aatatatata 300
aangttcaat tactagtaac accttattac agatacagat tacaacttag gaaatatatt 360
ttcatggacc attgatgtca tttggattcn cccctacaat c 401
<210>25
<211>1820
<212>DNA
<213>人
<400>25
aatgtcttag aaaaaggctt tctaaaagaa aaagagcaag aggccatttc ttttcaagat 60
agatacaaag aacttcagga aaaacataaa caagaattgg aagacatgag gaaagctggt 120
cacgaagccc tcagcattat tgtggatgaa tataaggcac tactgcagtc ttcagttaag 180
caacaagtag aagctattga aaaacagtac atttctgcaa ttgagaaaca ggcacacaag 240
tgtgaggagt tgctaaatgc tcagcatcag aggctccttg aaatgctaga tacagagaag 300
gaactgttaa aagaaaaaat aaaggaagct ttgattcagc aatctcaaga acagaaggaa 360
atattggaaa agtgtttgga ggaagaaagg caaagaaata aagaggcatt agtatccgct 420
gcaaagcttg aaaaagaagc agtgaaggat gcagttttaa aagtcgtaga agaagaaaga 480
aaaaatttag aaaaagcgca tgctgaagaa agggaattat ggaagacaga acatgcaaaa 540
gatcaagaaa aagtatctca ggaaattcaa aaagctatac aagaacaaag aaaaataagt 600
caggaaactg ttaaggcagc aataatagaa gagcagaaac gaagtgaaaa ggctgtggaa 660
gaggcagtga aaagaacaag agatgaattg atagagtata taaaagaaca gaaaaggctc 720
gatcaagtca tccgccaaag aagcctgtcc agtttggaac tgttcctctc ctgtgcacag 780
aaacagttaa gtgctttaat agctacggaa ccagttgaca ttgaataaaa agaacatgac 840
aaacccacac tggcattgga taaatcatat tacaccttca aaatacacac tctgaattat 900
aaagatgtgt ttgttttctt tccaaatcat gtagaattga tttccagttc aaggataaac 960
caaaacaata tttagaacta tcaagtgatc taatttattt tcttttggtt tcttctttac 1020
atttactgtt attttattat tattagtagt agcagcaaca gagtatgata tgacccaaaa 1080
gccattgtaa agtgccacat taccaaaatt aattaagtaa actttatagc ctgtgggagt 1140
ctattatata ttattttgca aaagtagtaa atatattatt gtttcatgat gactcttgat 1200
gagatgctag aatgtaacca tacatttatc ttattttgag gatagaaata gcatggattt 1260
caacatcact tatttatctg tataattgga aataaaacac cgatatgata gagaatcatt 1320
ccggcattac ctaacctctt ctgcagttgg atctatgtat tttcattggt ctactgaaaa 1380
cgaacaatac aattaaaagc actaaagatt attatattaa ttcaactttg atctgatata 1440
tcacttaaac taaaggggtg tgtgtggtgt atgcttgttt cctatttctg ctctttaaag 1500
atactttgaa tcaataaaac cattagtcta caaatcaaat tgtgaactta atctctagaa 1560
agagaatata actcagccat ttataggaat ttaggttcaa gtacaggata tatgaaatct 1620
tttcccagta tttcagaatg tacttaattc acaggcagga tgcttcaatg caaaatcatg 1680
aatattttta attcaaaact aaaatgtcat taatatgtat gtatgcaaat gttttatctt 1740
attttctgaa atgcatctac tttcatgggc tttgtacgtt tctgagattt ctcagtgtaa 1800
taaaaagagc tcccaaactt 1820
<210>26
<211>280
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(261)..(261)
<223>任意碱基
<220>
<221>misc_特征
<222>(237)..(237)
<223>任意碱基
<400>26
tcaagtcata agataaagtt taatcatttg atcatgttaa aagacacaaa acacagccaa 60
tctaaccaaa tttcaggcat gcatttacat aaatatatta aattaagaaa agaaattgta 120
cacttaaacg tccttttcac ctagaaatca ttaaatccac agatcaacaa taaaaccaat 180
tctctgcatt taccacttca agatacaatt gttctatttt aaagataaca caaactncac 240
tagtctggtt aggaatttat ntgcattata catatattat 280
<210>27
<211>392
<212>DNA
<213>人
<400>27
ttggtttgaa atggcacccc aggactttgg gcctgcctta cttgatagcc tcgttcagtg 60
agcaaagact tagtgagcag ctcttgtatg ccaagtattt tgctaagctc tggaaaaaag 120
ataaacaaga catggttctt gctttcaagg agtgtgtaat tctttagcca gatatggaaa 180
cctggaccct gagtgggaga aaggagacag atgaaaggag tccgtgattt tgtaaccaag 240
agctgcctgc atggttatga gtatcactga ttttagggac gcccacagag ctaaagcatt 300
tttttaatcc gagaagactt ttgtaactca tattagttaa tcttctagct ctgagatagc 360
aacacagctc ttagaattct gtaagtaagc tt 392
<210>28
<211>2299
<212>DNA
<213>人
<400>28
cgaaccccca cagctggagg gcgaggccag ctgtacccgg ccccagtgcc ctttcgcggc 60
cacaagcggc cgtcctcctg gtccggtgct ccggcgcctg atctaggttc atggagccgg 120
ggctgtggct ccttttcggg ctcacagtga cctccgccgc aggattcgtg ccttgctccc 180
agtctgggga tgctggcagg cgcggcgtgt cccaggcccc cactgcagcc agatctgagg 240
gggactgtga agagactgtg gctggccctg gcgaggagac tgtggctggc cctggcgagg 300
ggactgtggc cccgacagca ctgcagggtc caagccctgg aagccctggg caggagcagg 360
cggccgaggg ggcccctgag caccaccgat ccaggcgctg cacgtgcttc acctacaagg 420
acaaggagtg tgtctactat tgccacctgg acatcatttg gatcaacact cccgaacaga 480
cggtgcccta tggactgtcc aactacagag gaagcttccg gggcaagagg tctgcggggc 540
cacttccagg gaatctgcag ctctcacatc ggccacactt gcgctgcgct tgtgtgggga 600
gatatgacaa ggcctgcctg cacttttgca cccaaactct ggacgtcagc agacaggttg 660
aagtcaagga ccaacaaagc aagcaggctt tagacctcca ccatccaaag ctcatgcccg 720
gcagtggact cgccctcgct ccatctacct gcccccgctg cctctttcag gaaggagccc 780
cttaggagga caggcctgca gcatcctggt ctcgggaggc ttctgtcatt gctcacacac 840
agttcagatt tccacctctt tatagacaag aagtgaattt gcctggggca gaacacccac 900
ccaaagagtc cccacttaac aatacccccc ccccacggca agaatgccca aatccgaatg 960
accccagttt tcctaatgag taaaatgatc ccagatgtgc cccagagcat gacgcctgca 1020
gctccggttt catgcaggaa attggttttg gagagttttg gcaagttgga aagccactta 1080
ctggcttttg acatgacttc tcttggagaa taagtggact ccaagctaac tctttgcaaa 1140
tgtaaacaca tgtccatctt gtaataaatg caaaatgccc gtgcagcaga agcatgcgac 1200
tttcatatcc ttgcctagaa taggctgcat ggtgtatgtc agtgagggcc acgaggcgtc 1260
ggctttagac acagatcata gctctacagg agtttatgaa tttgaagctt atgggatttt 1320
ggcagagaaa ttttcagctg tgcttgatac ccaccaaaag aatgtatctc gaaagaatga 1380
aggaagaaga aaaaaggatc cttgatgttt gtgacaagaa aatgagaaag ttagtatctg 1440
caatacagag cttgttcctg ttcagtgact gaccctctgt attctgtata gacaccaggc 1500
cgatacacag tggagttccc aggccttgtt tgcaggaagc cgactgtaaa gacagcccca 1560
gctcaaggct attaggttga atatttgctt tcatgagtaa atgtggatct ttggggaatg 1620
gcttcaaaat aagtcacgaa cacaaattct ttgtaaatta tgtaaattcc tgtttatata 1680
aattggcaac aacttatacc gtctgacagt tcaaaatctc tttcagctgc gctcttccca 1740
ccgagccgag cttactgtga gtgtggagat gttatcccac catgtaaagt cgcctgcgca 1800
ggggagggct gcccatctcc ccaacccagt cacagagaga taggaaacgg catttgagtg 1860
ggtgtccagg gccccgtaga gagacattta agatggtgta tgacagagca ttggccttga 1920
ccaaatgtta aatcctctgt gtgtatttca taagttatta caggtataaa agtgatgacc 1980
tatcatgagg aaatgaaagt ggctgatttg ctggtaggat tttgtacagt ttagagaagc 2040
gattatttat tgtgaaactg ttctccactc caactccttt atgtggatct gttcaaagta 2100
gtcactgtat atacgtatag agaggtagat aggtaggtag attttaaatt gcattctgaa 2160
tacaaactca tactccttag agcttgaatt acatttttaa aatgcatatg tgctgtttgg 2220
caccgtggca agatggtatc agagagaaac ccatcaattg ctcaaatact cagaaagtac 2280
tgtcaaaagc ctaataaaa 2299
<210>29
<211>1339
<212>DNA
<213>人
<400>29
ctaaacaaaa tcattcactt ccctgatttt gataagaaaa ttcctgtaaa gctgtttcct 60
ctgcctctcc tctacgttgg aaaccacata agtggattat caagcacaag taaattaagc 120
ctaccgatgt tcaccgtgct caggaaattc accattccac ttaccttact tctggaaacc 180
atcatacttg ggaagcagta ttcactcaac atcatcctca gtgtctttgc cattattctc 240
ggggctttca tagcagctgg gtctgacctt gcttttaact tagaaggcta tatttttgta 300
ttcctgaatg atatcttcac atcagcaaat ggagtttata ccaaacagaa aatggaccca 360
aaggagctag ggaaatacgg agtacttttc tacaatgcct gcttcatgat tatcccaact 420
cttattatta gtgtctccac tggagacctc caacaggcta ctgaattcaa ccaatggaag 480
aatgttgtgt ttatcctaca gtttcttctt tcctgttttt tggggtttct gctgatgtac 540
tccacggttc tgtgcagcta ttacaattca gccctgacga cagcagtggt tggagccatc 600
aagaatgtat ccgttgccta cattgggata ttaatcggtg gagactacat tttctctttg 660
ttaaactttg tagggttaaa tatttgcatg gcagggggct tgagatattc ctttttaaca 720
ctgagcagcc agttaaaacc taaacctgtg ggtgaagaaa acatctgttt ggatttgaag 780
agctaaagag tctgcagcag gattggagac tgacttgtga ctgcgggctg ggggggcatt 840
cccagtagga atgtgaagcc agaggtttcg gattcgtgac atccaccccc tgggcaagtg 900
agagcatctg caaaatgcaa agagaactac ctcatatgca ggatgagcca atggcagtct 960
caagaaatgt actcgggcga caccttacct gtggaaagca aatcttttca aaataagcca 1020
ctgggactcg gtaggtggag ccccagctgc tcttctaggg acctatgggg ccttcgtggc 1080
atctctgtgc tgtgtgctgg ggaggaggtt gatgtaatgg tgactctttt ctgatcagca 1140
ccttggccgt gattcccaag gtcccagcca aagcaaaggg ccagttgttt cagtttaaac 1200
agacatgtct ttagtctaat aaaattagtt aactgccagt aaagttattt gttagctttg 1260
atgaaagcta tgttggtatc tttccctaat catcaaagta aataaaaaat catttctatg 1320
taaaaaaaaa aaaaaaaaa 1339
<210>30
<211>4250
<212>DNA
<213>人
<400>30
gaacacatcg cgtttgcatc ccagaaagta gtcgccgcga ctatttcccc caaagagaca 60
agcacacatg taggaatgac aaaggcttgc gaaggagaga gcgcagcccg cggcccggag 120
agatcccctc gataatggat tactaaatgg gatacacgct gtaccagttc gctccgagcc 180
ccggccgcct gtccgtcgat gcaccgaaaa gggtgaagta gagaaataaa gtctccccgc 240
tgaactacta tgaggtcaga agccttgctg ctatatttca cactgctaca ctttgctggg 300
gctggtttcc cagaagattc tgagccaatc agtatttcgc atggcaacta tacaaaacag 360
tatccggtgt ttgtgggcca caagccagga cggaacacca cacagaggca caggctggac 420
atccagatga ttatgatcat gaacggaacc ctctacattg ctgctaggga ccatatttat 480
actgttgata tagacacatc acacacggaa gaaatttatt gtagcaaaaa actgacatgg 540
aaatctagac aggccgatgt agacacatgc agaatgaagg gaaaacataa ggatgagtgc 600
cacaacttta ttaaagttct tctaaagaaa aacgatgatg cattgtttgt ctgtggaact 660
aatgccttca acccttcctg cagaaactat aagatggata cattggaacc attcggggat 720
gaattcagcg gaatggccag atgcccatat gatgccaaac atgccaacgt tgcactgttt 780
gcagatggaa aactatactc agccacagtg actgacttcc ttgccattga cgcagtcatt 840
taccggagtc ttggagaaag ccctaccctg cggaccgtca agcacgattc aaaatggttg 900
aaagaaccat actttgttca agccgtggat tacggagatt atatctactt cttcttcagg 960
gaaatagcag tggagtataa caccatggga aaggtagttt tcccaagagt ggctcaggtt 1020
tgtaagaatg atatgggagg atctcaaaga gtcctggaga aacagtggac gtcgttcctg 1080
aaggcgcgct tgaactgctc agttcctgga gactctcatt tttatttcaa cattctccag 1140
gcagttacag atgtgattcg tatcaacggg cgtgatgttg tcctggcaac gttttctaca 1200
ccttataaca gcatccctgg gtctgcagtc tgtgcctatg acatgcttga cattgccagt 1260
gtttttactg ggagattcaa ggaacagaag tctcctgatt ccacctggac accagttcct 1320
gatgaacgag ttcctaagcc caggccaggt tgctgtgctg gctcatcctc cttagaaaga 1380
tatgcaacct ccaatgagtt ccctgatgat accctgaact tcatcaagac gcacccgctc 1440
atggatgagg cagtgccctc catcttcaac aggccatggt tcctgagaac aatggtcaga 1500
taccgcctta ccaaaattgc agtggacaca gctgctgggc catatcagaa tcacactgtg 1560
gtttttctgg gatcagagaa gggaatcatc ttgaagtttt tggccagaat aggaaatagt 1620
ggttttctaa atgacagcct tttcctggag gagatgagtg tttacaactc tgaaaaatgc 1680
agctatgatg gagtcgaaga caaaaggatc atgggcatgc agctggacag agcaagcagc 1740
tctctgtatg ttgcgttctc tacctgtgtg ataaaggttc cccttggccg gtgtgaacga 1800
catgggaagt gtaaaaaaac ctgtattgcc tccagagacc catattgtgg atggataaag 1860
gaaggtggtg cctgcagcca tttatcaccc aacagcagac tgacttttga gcaggacata 1920
gagcgtggca atacagatgg tctgggggac tgtcacaatt cctttgtggc actgaatgac 1980
atttcaactc ctctaccaga taatgaaatg tcttacaaca cagtgtatgg gcattccagt 2040
tccctcttgc ccagcacaac cacatcagat tcgacggctc aagaggggta tgagtctagg 2100
ggaggaatgc tggactggaa gcatctgctt gactcacctg acagcacaga ccctttgggg 2160
gcagtgtctt cccataatca ccaagacaag aagggagtga ttcgggaaag ttacctcaaa 2220
ggccacgacc agctggttcc cgtcaccctc ttggccattg cagtcatcct ggctttcgtc 2280
atgggggccg tcttctcggg catcaccgtc tactgcgtct gtgatcatcg gcgcaaagac 2340
gtggctgtgg tgcagcgcaa ggagaaggag ctcacccact cgcgccgggg ctccatgagc 2400
agcgtcacca agctcagcgg cctctttggg gacactcaat ccaaagaccc aaagccggag 2460
gccatcctca cgccactcat gcacaacggc aagctcgcca ctcccggcaa cacggccaag 2520
atgctcatta aagcagacca gcaccacctg gacctgacgg ccctccccac cccagagtca 2580
accccaacgc tgcagcagaa gcggaagccc agccgcggca gccgcgagtg ggagaggaac 2640
cagaacctca tcaatgcctg cacaaaggac atgcccccca tgggctcccc tgtgattccc 2700
acggacctgc ccctgcgggc ctcccccagc cacatcccca gcgtggtggt cctgcccatc 2760
acgcagcagg gctaccagca tgagtacgtg gaccagccca aaatgagcga ggtggcccag 2820
atggcgctgg aggaccaggc cgccacactg gagtataaga ccatcaagga acatctcagc 2880
agcaagagtc ccaaccatgg ggtgaacctt gtggagaacc tggacagcct gccccccaaa 2940
gttccacagc gggaggcctc cctgggtccc ccgggagcct ccctgtctca gaccggtcta 3000
agcaagcggc tggaaatgca ccactcctct tcctacgggg ttgactataa gaggagctac 3060
cccacgaact cgctcacgag aagccaccag gccaccactc tcaaaagaaa caacactaac 3120
tcctccaatt cctctcacct ctccagaaac cagagctttg gcaggggaga caacccgccg 3180
cccgccccgc agagggtgga ctccatccag gtgcacagct cccagccatc tggccaggcc 3240
gtgactgtct cgaggcagcc cagcctcaac gcctacaact cactgacaag gtcggggctg 3300
aagcgtacgc cctcgctaaa gccggacgta ccccccaaac catcctttgc tcccctttcc 3360
acatccatga agcccaatga tgcgtgtaca taatcccagg gggagggggt caggtgtcga 3420
accagcaggc aaggcgaggt gcccgctcag ctcagcaagg ttctcaactg cctcgagtac 3480
ccaccagacc aagaaggcct gcggcagagc cgaggacgct gggtcctcct ctctgggaca 3540
caggggtact cacgaaaact gggccgcgtg gtttggtgaa ggtttgcaac ggcggggact 3600
caccttcatt ctcttccttc actttccccc acaccctaca acaggtcgga cccacaaaag 3660
acttcagtta tcatcacaaa catgagccaa aagcacatac ctaccccatc ccccaccccc 3720
acacacacac acacatgcac acaacacata cacacacacg cacagaggtg aacagaaact 3780
gaaacatttt gtccacaact tcacgggacg tggccagact gggtttgcgt tccaacctgc 3840
aaaacacaaa tacatttttt aaaatcaaga aaatttaaaa agacaaaaaa aaaagaattc 3900
attgataatt ctaactcaga ctttaacaat ggcagaagtt tactatgcgc aaatactgtg 3960
aaatgcccgc cagtgttaca gctttctgtt gcagcagata aatgccatgt tgggcaacta 4020
tgtcatagat ttctgctcct cctctctttt aatgaaataa cgtgaccgtt aacgcaagta 4080
actctttatt tattgttcac cctttttttc cttaaggaaa ggactcttcc aaatatcatc 4140
ctatgaacag ctcttcagaa agcccattga aagttaaact atttaacgtg aaatccatta 4200
actggaataa ttgagtttct ttatttttac aataaattca ctgagtaaat 4250
<210>31
<211>2785
<212>DNA
<213>人
<400>31
ctttagccca acagtcaaaa ataattgatg ctaccctaca aatgtccaaa actctagtat 60
atcatatttc taagttacag caaatattag tcctgctaaa ccagggagct ttggcaaaaa 120
tgttttttga cagtaaattt gtccttgatt atatattaac tagtcaaaga ggtgtttgta 180
acattattag agcttcttgt tgtaggtggg ttaacaccac caatcaagag gtcattctaa 240
cagaaagcct ggatcagaaa accatcaccc taaaaaaaca tgccttacat atttaacaca 300
ctctgaaatc cagtcaaaat atgactaaag gcccttgcca tgactgatgt attctcctgg 360
ccaacgccaa acaaatggga gcctggttac gagtcagcct tcagggactt gtcacatttc 420
tacttggttt cttccttgtt attgtcataa taaaatgttt tctatgctgt ttagtgcaac 480
ttaggcccta ttctgtagaa gtctcctcta ctattcaggc cactcaaaca ccccaaataa 540
ttgagttcaa aatcgacatc aagatataaa ggaatcagtg actaaatata tttcatatat 600
ggtattttta ttgattattg tgctgtcttg acctagtatg gaggccttgg ctagaggctg 660
gtcagtttcc tctcttgagc agctgattaa atccacaccc caaccacttc ccttatcagg 720
ttctcacact ctggggccac tatgtaccca ctctaatcac cacagggcca gacatcagac 780
aattaaggac agcgcccatg ccccaaagcc cgccaaaatt atgcaaatta ttcaaaatta 840
ttcaacctag ctaaccccac cctttttgct gtacataagc tgcccattcc ccctccagcc 900
tgtggtaccc agtcctcagg tgcaaccccc tgcgtggtcc tctgtggcag ccttctctca 960
ttcagagctg ttttccacag aggtagtgaa aagaactgga ttttcaagtt cactttgcaa 1020
gagaaaaaga aaactcagta gaagataatg gcaagtccag actggggata tgatgacaaa 1080
aatggtcctg aacaatggag caagctgtat cccattgcca atggaaataa ccaatcccct 1140
gttgatatta aaaccagtga aaccaaacat gacacctctc tgaaacctat tagtgtctcc 1200
tacaacccag ccacagccaa agaaattatc aatgtggggc attctttcca tgtaaatttt 1260
gaggacaacg ataaccgatc agtgctgaaa ggtggtcctt tctctgacag ctacaggctc 1320
tttcagtttc attttcactg gggcagtaca aatgagcatg gttcagaaca tacagtggat 1380
ggagtcaaat attctgccga gcttcacgta gctcactgga attctgcaaa gtactccagc 1440
cttgctgaag ctgcctcaaa ggctgatggt ttggcagtta ttggtgtttt gatgaaggtt 1500
ggtgaggcca acccaaagct gcagaaagta cttgatgccc tccaagcaat taaaaccaag 1560
ggcaaacgag ccccattcac aaattttgac ccctctactc tccttccttc atccctggat 1620
ttctggacct accctggctc tctgactcat cctcctcttt atgagagtgt aacttggatc 1680
atctgtaagg agagcatcag tgtcagctca gagcagctgg cacaattccg cagccttcta 1740
tcaaatgttg aaggtgataa cgctgtcccc atgcagcaca acaaccgccc aacccaacct 1800
ctgaagggca gaacagtgag agcttcattt tgatgattct gagaagaaac ttgtccttcc 1860
tcaagaacac agccctgctt ctgacataat ccagttaaaa taataatttt taagaaataa 1920
atttatttca atattagcaa gacagcatgc cttcaaatca atctgtaaaa ctaagaaact 1980
taaattttag ttcttactgc ttaattcaaa taataattag taagctagca aatagtaatc 2040
tgtaagcata agcttatctt aaattcaagt ttagtttgag gaattcttta aaattacaac 2100
taagtgattt gtatgtctat ttttttcagt ttatttgaac caataaaata attttatctc 2160
tttctttctg ttgtgcattc agtttctaaa accattaagt ttctactcca tttacattca 2220
aaaatcttaa atactttact tgcaagagta ttttgcttca aatacaacaa cctaagagca 2280
gctggagatg aaatattggg aaattcattt gcttactcct gaagacaaaa atatagctga 2340
gatgaccact ggatttaata tcgttatgct ggcccaacat tgctaccatt tgtgttgtct 2400
gtgatcaaaa tgattatctt ttatatagga agatgacgct tctggatatt gctttcactt 2460
cttctcccca cgttagcaag gacaatgctt ctctgccatt attacaacta gttagtttgc 2520
atggagaatc tttactttaa aattggaaga aaagtcacaa gtgaatggtt tataaaaatg 2580
ctaaagaagt cattcttgct tagaatcata tagaaacatc atgcaatctt ttagtcagat 2640
gtgcgcttca ccttatgcta tttttatctt taattgacac acaataattg tacatgttta 2700
tggagtatag tgtggtgttt tctgtttgtt tgtttgtttt ttgagacaag gtctcactct 2760
gccagtcagg gtggagtgcg atggt 2785
<210>32
<211>9588
<212>DNA
<213>人
<400>32
ccgaccaaca ccaacaccca gctccgacgc agctcctctg cgcccttgcc gccctccgag 60
ccacagcttt cctcccgctc ctgcccccgg cccgtcgccg tctccgcgct cgcagcggcc 120
tcgggagggc ccaggtagcg agcagcgacc tcgcgagcct tccgcactcc cgcccggttc 180
cccggccgtc cgcctatcct tggccccctc cgctttctcc gcgccggccc gcctcgctta 240
tgcctcggcg ctgagccgct ctcccgattg cccgccgaca tgagctgcaa cggaggctcc 300
cacccgcgga tcaacactct gggccgcatg atccgcgccg agtctggccc ggacctgcgc 360
tacgaggtga ccagcggcgg cgggggcacc agcaggatgt actattctcg gcgcggcgtg 420
atcaccgacc agaactcgga cggctactgt caaaccggca cgatgtccag gcaccagaac 480
cagaacacca tccaggagct gctgcagaac tgctccgact gcttgatgcg agcagagctc 540
atcgtgcagc ctgaattgaa gtatggagat ggaatacaac tgactcggag tcgagaattg 600
gatgagtgtt ttgcccaggc caatgaccaa atggaaatcc tcgacagctt gatcagagag 660
atgcggcaga tgggccagcc ctgtgatgct taccagaaaa ggcttcttca gctccaagag 720
caaatgcgag ccctttataa agccatcagt gtccctcgag tccgcagggc cagctccaag 780
ggtggtggag gctacacttg tcagagtggc tctggctggg atgagttcac caaacatgtc 840
accagtgaat gtttggggtg gatgaggcag caaagggcgg agatggacat ggtggcctgg 900
ggtgtggacc tggcctcagt ggagcagcac attaacagcc accggggcat ccacaactcc 960
atcggcgact atcgctggca gctggacaaa atcaaagccg acctgcgcga gaaatctgcg 1020
atctaccagt tggaggagga gtatgaaaac ctgctgaaag cgtcctttga gaggatggat 1080
cacctgcgac agctgcagaa catcattcag gccacgtcca gggagatcat gtggatcaat 1140
gactgcgagg aggaggagct gctgtacgac tggagcgaca agaacaccaa catcgctcag 1200
aaacaggagg ccttctccat acgcatgagt caactggaag ttaaagaaaa agagctcaat 1260
aagctgaaac aagaaagtga ccaacttgtc ctcaatcagc atccagcttc agacaaaatt 1320
gaggcctata tggacactct gcagacgcag tggagttgga ttcttcagat caccaagtgc 1380
attgatgttc atctgaaaga aaatgctgcc tactttcagt tttttgaaga ggcgcagtct 1440
actgaagcat acctgaaggg gctccaggac tccatcagga agaagtaccc ctgcgacaag 1500
aacatgcccc tgcagcacct gctggaacag atcaaggagc tggagaaaga acgagagaaa 1560
atccttgaat acaagcgtca ggtgcagaac ttggtaaaca agtctaagaa gattgtacag 1620
ctgaagcctc gtaacccaga ctacagaagc aataaaccca ttattctcag agctctctgt 1680
gactacaaac aagatcagaa aatcgtgcat aagggggatg agtgtatcct gaaggacaac 1740
aacgagcgca gcaagtggta cgtgacgggc ccgggaggcg ttgacatgct tgttccctct 1800
gtggggctga tcatccctcc tccgaaccca ctggccgtgg acctctcttg caagattgag 1860
cagtactacg aagccatctt ggctctgtgg aaccagctct acatcaacat gaagagcctg 1920
gtgtcctggc actactgcat gattgacata gagaagatca gggccatgac aatcgccaag 1980
ctgaaaacaa tgcggcagga agattacatg aagacgatag ccgaccttga gttacattac 2040
caagagttca tcagaaatag ccaaggctca gagatgtttg gagatgatga caagcggaaa 2100
atacagtctc agttcaccga tgcccagaag cattaccaga ccctggtcat tcagctccct 2160
ggctatcccc agcaccagac agtgaccaca actgaaatca ctcatcatgg aacctgccaa 2220
gatgtcaacc ataataaagt aattgaaacc aacagagaaa atgacaagca agaaacatgg 2280
atgctgatgg agctgcagaa gattcgcagg cagatagagc actgcgaggg caggatgact 2340
ctcaaaaacc tccctctagc agaccagggg tcttctcacc acatcacagt gaaaattaac 2400
gagcttaaga gtgtgcagaa tgattcacaa gcaattgctg aggttctcaa ccagcttaaa 2460
gatatgcttg ccaacttcag aggttctgaa aagtactgct atttacagaa tgaagtattt 2520
ggactatttc agaaactgga aaatatcaat ggtgttacag atggctactt aaatagctta 2580
tgcacagtaa gggcactgct ccaggctatt ctccaaacag aagacatgtt aaaggtttat 2640
gaagccaggc tcactgagga ggaaactgtc tgcctggacc tggataaagt ggaagcttac 2700
cgctgtggac tgaagaaaat aaaaaatgac ttgaacttga agaagtcgtt gttggccact 2760
atgaagacag aactacagaa agcccagcag atccactctc agacttcaca gcagtatcca 2820
ctttatgatc tggacttggg caagttcggt gaaaaagtca cacagctgac agaccgctgg 2880
caaaggatag ataaacagat cgactttaga ttatgggacc tggagaaaca aatcaagcaa 2940
ttgaggaatt atcgtgataa ctatcaggct ttctgcaagt ggctctatga tcgtaaacgc 3000
cgccaggatt ccttagaatc catgaaattt ggagattcca acacagtcat gcggtttttg 3060
aatgagcaga agaacttgca cagtgaaata tctggcaaac gagacaaatc agaggaagta 3120
caaaaaattg ctgaactttg cgccaattca attaaggatt atgagctcca gctggcctca 3180
tacacctcag gactggaaac tctgctgaac atacctatca agaggaccat gattcagtcc 3240
ccttctgggg tgattctgca agaggctgca gatgttcatg ctcggtacat tgaactactt 3300
acaagatctg gagactatta caggttctta agtgagatgc tgaagagttt ggaagatctg 3360
aagctgaaaa ataccaagat cgaagttttg gaagaggagc tcagactggc ccgagatgcc 3420
aactcggaaa actgtaataa gaacaaattc ctggatcaga acctgcagaa ataccaggca 3480
gagtgttccc agttcaaagc gaagcttgcg agcctggagg agctgaagag acaggctgag 3540
ctggatggga agtcggctaa gcaaaatcta gacaagtgct acggccaaat aaaagaactc 3600
aatgagaaga tcacccgact gacttatgag attgaagatg aaaagagaag aagaaaatct 3660
gtggaagaca gatttgacca acagaagaat gactatgacc aactgcagaa agcaaggcaa 3720
tgtgaaaagg agaaccttgg ttggcagaaa ttagagtctg agaaagccat caaggagaag 3780
gagtacgaga ttgaaaggtt gagggttcta ctgcaggaag aaggcacccg gaagagagaa 3840
tatgaaaatg agctggcaaa ggtaagaaac cactataatg aggagatgag taatttaagg 3900
aacaagtatg aaacagagat taacattacg aagaccacca tcaaggagat atccatgcaa 3960
aaagaggatg attccaaaaa tcttagaaac cagcttgata gactttcaag ggaaaatcga 4020
gatctgaagg atgaaattgt caggctcaat gacagcatct tgcaggccac tgagcagcga 4080
aggcgagctg aagaaaacgc ccttcagcaa aaggcctgtg gctctgagat aatgcagaag 4140
aagcagcatc tggagataga actgaagcag gtcatgcagc agcgctctga ggacaatgcc 4200
cggcacaagc agtccctgga ggaggctgcc aagaccattc aggacaaaaa taaggagatc 4260
gagagactca aagctgagtt tcaggaggag gccaagcgcc gctgggaata tgaaaatgaa 4320
ctgagtaagg taagaaacaa ttatgatgag gagatcatta gcttaaaaaa tcagtttgag 4380
accgagatca acatcaccaa gaccaccatc caccagctca ccatgcagaa ggaagaggat 4440
accagtggct accgggctca gatagacaat ctcacccgag aaaacaggag cttatctgaa 4500
gaaataaaga ggctgaagaa cactctaacc cagaccacag agaatctcag gagggtggaa 4560
gaagacatcc aacagcaaaa ggccactggc tctgaggtgt ctcagaggaa acagcagctg 4620
gaggttgagc tgagacaagt cactcagatg cgaacagagg agagcgtaag atataagcaa 4680
tctcttgatg atgctgccaa aaccatccag gataaaaaca aggagataga aaggttaaaa 4740
caactgatcg acaaagaaac aaatgaccgg aaatgcctgg aagatgaaaa cgcgagatta 4800
caaagggtcc agtatgacct gcagaaagca aacagtagtg cgacggagac aataaacaaa 4860
ctgaaggttc aggagcaaga actgacacgc ctgaggatcg actatgaaag ggtttcccag 4920
gagaggactg tgaaggacca ggatatcacg cggttccaga actctctgaa agagctgcag 4980
ctgcagaagc agaaggtgga agaggagctg aatcggctga agaggaccgc gtcagaagac 5040
tcctgcaaga ggaagaagct ggaggaagag ctggaaggca tgaggaggtc gctgaaggag 5100
caagccatca aaatcaccaa cctgacccag cagctggagc aggcatccat tgttaagaag 5160
aggagtgagg atgacctccg gcagcagagg gacgtgctgg atggccacct gagggaaaag 5220
cagaggaccc aggaagagct gaggaggctc tcttctgagg tcgaggccct gaggcggcag 5280
ttactccagg aacaggaaag tgtcaaacaa gctcacttga ggaatgagca tttccagaag 5340
gcgatagaag ataaaagcag aagcttaaat gaaagcaaaa tagaaattga gaggctgcag 5400
tctctcacag agaacctgac caaggagcac ttgatgttag aagaagaact gcggaacctg 5460
aggctggagt acgatgacct gaggagagga cgaagcgaag cggacagtga taaaaatgca 5520
accatcttgg aactaaggag ccagctgcag atcagcaaca accggaccct ggaactgcag 5580
gggctgatta atgatttaca gagagagagg gaaaatttga gacaggaaat tgagaaattc 5640
caaaagcagg ctttagaggc atctaatagg attcaggaat caaagaatca gtgtactcag 5700
gtggtacagg aaagagagag ccttctggtg aaaatcaaag tcctggagca agacaaggca 5760
aggctgcaga ggctggagga tgagctgaat cgtgcaaaat caactctaga ggcagaaacc 5820
agggtgaaac agcgcctgga gtgtgagaaa cagcaaattc agaatgacct gaatcagtgg 5880
aagactcaat attcccgcaa ggaggaggct attaggaaga tagaatcgga aagagaaaag 5940
agtgagagag agaagaacag tcttaggagt gagatcgaaa gactccaagc agagatcaag 6000
agaattgaag agaggtgcag gcgtaagctg gaggattcta ccagggagac acagtcacag 6060
ttagaaacag aacgctcccg atatcagagg gagattgata aactcagaca gcgcccatat 6120
gggtcccatc gagagaccca gactgagtgt gagtggaccg ttgacacctc caagctggtg 6180
tttgatgggc tgaggaagaa ggtgacagca atgcagctct atgagtgtca gctgatcgac 6240
aaaacaacct tggacaaact attgaagggg aagaagtcag tggaagaagt tgcttctgaa 6300
atccagccat tccttcgggg tgcaggatct atcgctggag catctgcttc tcctaaggaa 6360
aaatactctt tggtagaggc caagagaaag aaattaatca gcccagaatc cacagtcatg 6420
cttctggagg cccaggcagc tacaggtggt ataattgatc cccatcggaa tgagaagctg 6480
actgtcgaca gtgccatagc tcgggacctc attgacttcg atgaccgtca gcagatatat 6540
gcagcagaaa aagctatcac tggttttgat gatccatttt caggcaagac agtatctgtt 6600
tcagaagcca tcaagaaaaa tttgattgat agagaaaccg gaatgcgcct gctggaagcc 6660
cagattgctt cagggggtgt agtagaccct gtgaacagtg tctttttgcc aaaagatgtc 6720
gccttggccc gggggctgat tgatagagat ttgtatcgat ccctgaatga tccccgagat 6780
agtcagaaaa actttgtgga tccagtcacc aaaaagaagg tcagttacgt gcagctgaag 6840
gaacggtgca gaatcgaacc acatactggt ctgctcttgc tttcagtaca gaagagaagc 6900
atgtccttcc aaggaatcag acaacctgtg accgtcactg agctagtaga ttctggtata 6960
ttgagaccgt ccactgtcaa tgaactggaa tctggtcaga tttcttatga cgaggttggt 7020
gagagaatta aggacttcct ccagggttca agctgcatag caggcatata caatgagacc 7080
acaaaacaga agcttggcat ttatgaggcc atgaaaattg gcttagtccg acctggtact 7140
gctctggagt tgctggaagc ccaagcagct actggcttta tagtggatcc tgttagcaac 7200
ttgaggttac cagtggagga agcctacaag agaggtctgg tgggcattga gttcaaagag 7260
aagctcctgt ctgcagaacg agctgtcact gggtataatg atcctgaaac aggaaacatc 7320
atctctttgt tccaagccat gaataaggaa ctcatcgaaa agggccacgg tattcgctta 7380
ttagaagcac agatcgcaac cggggggatc attgacccaa aggagagcca tcgtttacca 7440
gttgacatag catataagag gggctatttc aatgaggaac tcagtgagat tctctcagat 7500
ccaagtgatg ataccaaagg attttttgac cccaacactg aagaaaatct tacctatctg 7560
caactaaaag aaagatgcat taaggatgag gaaacagggc tctgtcttct gcctctgaaa 7620
gaaaagaaga aacaggtgca gacatcacaa aagaataccc tcaggaagcg tagagtggtc 7680
atagttgacc cagaaaccaa taaagaaatg tctgttcagg aggcctacaa gaagggccta 7740
attgattatg aaaccttcaa agaactgtgt gagcaggaat gtgaatggga agaaataacc 7800
atcacgggat cagatggctc caccagggtg gtcctggtag atagaaagac aggcagtcag 7860
tatgatattc aagatgctat tgacaagggc cttgttgaca ggaagttctt tgatcagtac 7920
cgatccggca gcctcagcct cactcaattt gctgacatga tctccttgaa aaatggtgtc 7980
ggcaccagca gcagcatggg cagtggtgtc agcgatgatg tttttagcag ctcccgacat 8040
gaatcagtaa gtaagatttc caccatatcc agcgtcagga atttaaccat aaggagcagc 8100
tctttttcag acaccctgga agaatcgagc cccattgcag ccatctttga cacagaaaac 8160
ctggagaaaa tctccattac agaaggtata gagcggggca tcgttgacag catcacgggt 8220
cagaggcttc tggaggctca ggcctgcaca ggtggcatca tccacccaac cacgggccag 8280
aagctgtcac ttcaggacgc agtctcccag ggtgtgattg accaagacat ggccaccagc 8340
gtgaagcctg ctcagaaagc cttcataggc ttcgagggtg tgaagggaaa gaagaagatg 8400
tcagcagcag aggcagtgaa agaaaaatgg ctcccgtatg aggctggcca gcgcttcctg 8460
gagttccagt acctcacggg aggtcttgtt gacccggaag tgcatgggag gataagcacc 8520
gaagaagcca tccggaaggg gttcatagat ggccgcgccg cacagaggct gcaagacacc 8580
agcagctatg ccaaaatcct gacctgcccc aaaaccaaat taaaaatatc ctataaggat 8640
gccataaatc gctccatggt agaagatatc actgggctgc gccttctgga agccgcctcc 8700
gtgtcgtcca agggcttacc cagcccttac aacatgtctt cggctccggg gtcccgctcc 8760
ggctcccgct cgggatctcg ctccggatct cgctccgggt cccgcagtgg gtcccggaga 8820
ggaagctttg acgccacagg gaattcttcc tactcttatt cctactcatt tagcagtagt 8880
tctattgggc actagtagtc agttgggagt ggttgctata ccttgacttc atttatatga 8940
atttccactt tattaaataa tagaaaagaa aatcccggtg cttgcagtag agtgatagga 9000
cattctatgc ttacagaaaa tatagccatg attgaaatca aatagtaaag gctgttctgg 9060
ctttttatct tcttagctca tcttaaataa gcagtacact tggatgcagt gcgtctgaag 9120
tgctaatcag ttgtaacaat agcacaaatc gaacttagga tttgtttctt ctcttctgtg 9180
tttcgatttt tgatcaattc tttaattttg gaagcctata atacagtttt ctattcttgg 9240
agataaaaat taaatggatc actgatattt tagtcattct gcttctcatc taaatatttc 9300
catattctgt attaggagaa aattaccctc ccagcaccag cccccctctc aaacccccaa 9360
cccaaaacca agcattttgg aatgagtctc ctttagtttc agagtgtgga ttgtataacc 9420
catatactct tcgatgtact tgtttggttt ggtattaatt tgactgtgca tgacagcggc 9480
aatcttttct ttggtcaaag ttttctgttt attttgcttg tcatattcga tgtactttaa 9540
ggtgtcttta tgaagtttgc tattctggca ataaactttt agactttt 9588
<210>33
<211>366
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(351)..(351)
<223>任意碱基
<400>33
gaagtgccat ttatatttat acaaaaatat tacataattc agttagtatt ggtgacataa 60
tttagttagt atgggtgata taatggtcat aatttttagc atctaataaa gatcttttta 120
tgagtcccat ataaaatatg tgaacaaagc aatcttgtca taagatttgt gatgatttag 180
gagaaagtac tttgagataa tttttttctg tctctttgtg aactctctca acagtagttc 240
tctttagatt agagccagca ggtcggccat aacagttttc ttcaaatttg ggcaacagtt 300
atacaaatgc ttgaatttca agacaacata ttaaagggtc tatgaactgg naatctaacc 360
tgggtt 366
<210>34
<211>1466
<212>DNA
<213>人
<400>34
agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60
cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120
gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180
cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240
catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300
aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360
gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420
gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480
agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540
gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600
agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660
aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720
cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780
ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840
aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900
ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960
gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc 1020
ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag 1080
gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg 1140
agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag 1200
gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct 1260
ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca 1320
tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg 1380
ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca 1440
cagaaataaa gagctgttat actgtg 1466
<210>35
<211>187
<212>DNA
<213>人
<400>35
gatctggtgc attccggtcg acactctcgt ttatttggac tgtaagtctg acctctatga 60
ataattactt cagcccctga gtgctcccgg gccaagctcc ttggccaaac tttcacctta 120
gcttctgata agtcttgggc caagctaagc agcatctatc aatcatccct tcagctcctg 180
attgatc 187
<210>36
<211>2913
<212>DNA
<213>人
<400>36
actgggtacc gaggactggg tgtgtttaag gcagacagcc aggtgaggat cccagctact 60
ggggcctgct gtcatctcct gggagtaccc gggggtcagg agcctagggg actcttgcac 120
ttcacatcca gccatgctaa ttacactttt tggcaaagga aacagctagg agcagtttct 180
ttcactccta cagccccgtt ttctcagtgt ttagacctcg aattattact gggctagagg 240
gaaggcagcc tctgaagtgt ggcaggagga ggggaagtct gcctgcatct tggtgtgtct 300
gtcagatgcc agcactaata acctggcttc tgtgaggcct gtcagtgctc tcaggaatga 360
aaggggaccc ctgagaggtg ctcagtacca gcaggctgtg aatgctctct acccaccacc 420
ctcacctcct cgttaaagat ggtgctacct gccacacagc agacatctgg tcgctgcaca 480
cccgaaagac cccaaggcag tctgcccctt gtccagccac acgccagcac ccaccctcct 540
ggcccctgcc tcggcctccc cagaccagct gcacccagcc cccaacacgc accccttctc 600
cagatgtgtg cagggcctca ttttgcagag caaagacaga tgtttcagcc acacgcttta 660
ttaacttcta aaacctgtgc tcaggacact cttcaacagt catgaaaagt ttgatcactt 720
gccacagtca ggacctttgt gtggggctct gatctgatgt tcggtctcat catctcccaa 780
accagcagtc gtttgtaccc caaccctctg ctcaggggct cataccccca aatgattttc 840
ctgatttatg tatttcccta caaagggctt tctataccta gcatctgcct ccagcatgag 900
aagggggaat aggtgagacc catttgccag tagcagacgg ggaccctggg gagaaaatgg 960
cagagcctgt tggagactcc ctgtctccag ctgaccagcc aatgggattc ctcttccctc 1020
cactgtctcc cacaaagtag aagaatcctg gtacatttag cccatgagcc tggcacagat 1080
ccctatctag acatgaggcc ctttagacat gactttggca ttgaccagcc tgttggcaat 1140
gggtcgggga ggcagagggg atgctcacac cagtaattct catcccctga atgcttggga 1200
tcacctgggg agagttcaca aaatactggt gcaggggtcc cacctctgat gatgctgagt 1260
ggtgggtctg gggtgtggcc caggcatcat gatgtttcag gcccccaggt gacttcttag 1320
gcagcccagc taagccccta gagccttgca atttccccca aatgacctca gagggcccga 1380
tttgagggaa atgcctaact tcaggggccg taagaatccc ccagggagca tgtgaaatgc 1440
agataccagg cccaccccca gagatgagct gaggtgggtc aggggtgaag tgcagggatc 1500
agtgtttttc acaagctcca tacctccagg aaatggtgtt gtggttgggc ccgtagaaaa 1560
cattctgaga gtcctgttgc ctgtgccttg gtgcacgtgg ggtggaatcc cagtggccct 1620
gccttgagga ggatgtgcat taacgtggta ggggagacag agacagctcc acctgccccc 1680
tgtcccaccg gggacctcca aaaacttcat ggatgttaga gcaagcagcc atgctgcagc 1740
agaggatgag gctggcggat ttagtaagag ccctctgtgt ttgggctgag ttctttctct 1800
agttgccctg tcatctggcc tctggataac ccacctctcc tccctcatcc taaaattaca 1860
gatggcgaaa gacggccaca tttagtgaga cccctaaggt cctccaacta gggtgggtcc 1920
acagtggccc ctggtgcatg gaccacacac tctcttccct cctctggctc aggactacgg 1980
tctgaaatta gggagatatg aatgtctttc ttgaaaactt ctcttcccag tcttcccact 2040
ttgcttgggg gtccttggtc aaggccagct ttggactagg gcttgttgcg actaccagct 2100
gtctcatttt gctgtactgc aaactcaggc ttggttccaa gcttatgggg gccctgtcct 2160
tcccctagta gggtttgttt tggggtcaca tctggtcata cccttcagag agctcttccc 2220
cagcctctac atcagggaga gaggtaggta gggaggagca ttcaaggatt agaagaagga 2280
ctaaagtaca acagccttgg aggaactgcc aggaactaag ggcgagcact ggagaaggca 2340
acctgggacc ccctgcgctt ctgagcagga agaccaagac cttcaggggc cctaagcact 2400
gaaaacatca ttcctcatcc ccaagccctg gcatccccct gttcttctaa aataattctt 2460
ttctaggtat ttctgattgc aaaattctgg atgggttcat ccaagctgac ctttgctgtt 2520
ttttcccttc ccaacaaggc ctcacttttt ggagccacct tagttggtgc ctaggcagag 2580
gggcagtcag cagtggttat caggatcctg gctctatggg ttgccttcct cctggtctgt 2640
aaagcccctg caggcaggga cttcttagat agctgcttcc ttagggcatg gcatgtggtg 2700
ggtggttaat gaatggaaga gagggaatga gtgatcaagg gagggaggag ggagtggagt 2760
ggagatttct catcctttcc tgttaattta tgacatcctc ctgcctatga gtccttgact 2820
ctggagtttt acaaagcagt cacatttcaa ataaaagtct gggaaagcaa cacatcatcg 2880
ccaactttta attttgctaa ataaggatat tag 2913
<210>37
<211>1466
<212>DNA
<213>人
<400>37
agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60
cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120
gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180
cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240
catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300
aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360
gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420
gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480
agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540
gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600
agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660
aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720
cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780
ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840
aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900
ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960
gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc 1020
ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag 1080
gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg 1140
agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag 1200
gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct 1260
ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca 1320
tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg 1380
ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca 1440
cagaaataaa gagctgttat actgtg 1466
<210>38
<211>462
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(197)..(197)
<223>任意碱基
<220>
<221>misc_特征
<222>(116)..(116)
<223>任意碱基
<220>
<221>misc_特征
<222>(334)..(334)
<223>任意碱基
<220>
<221>misc_特征
<222>(402)..(402)
<223>任意碱基
<220>
<221>misc_特征
<222>(429)..(429)
<223>任意碱基
<220>
<221>misc_特征
<222>(438)..(438)
<223>任意碱基
<220>
<221>misc_特征
<222>(443)..(443)
<223>任意碱基
<220>
<221>misc_特征
<222>(459)..(459)
<223>任意碱基
<400>38
taaggtttta taattatttt tatttttctt ttcttttttt tttatggctt ggatgacact 60
ttattttcag atccaatact agaagttgtt tccatgttca cattttcctt cctggnttaa 120
aaaaaagagt tgtatttttt ttttttgctt tttttaaatt atactttaag ttttagggta 180
catgtgcaca acgcagnggt tagctacata tgtatacatg tgccatgttg gcgtgctgca 240
tccagtaact cgtcatttaa cattaggtat atctccaaat gctatccttc cccccattgt 300
atttttcata gcttaaaaat cattgacata ggantaattc caactaaagt acggtattaa 360
atccctgggg gaataaattt tgtcttaaca agggtaaggt tngtgaaaag gatggttttg 420
tcacagggna aaaggganat ccncccattt taaaacccnc ct 462
<210>39
<211>1490
<212> DNA
<213>人
<400>39
ctcgtgcccc ccacggaggg gactgctctc ccccgctgca tcctttctgt gaggtacctt 60
acccacctca gcacctgaga gggtgaaata gaattctaac ctcgacattc gggaagtgtt 120
tttgagaagt ctcggtcggt aagggaagtc ttccaagtcc gtgcagcact aacgtattgg 180
cacctgcctc ctcttcggcc accccccaga tgaggcagct gtgactgtgt caagggaagc 240
cacgactctg accatagtct tctctcagct tccactgccg tctccacagg aaacccagaa 300
gttctgtgaa caagtccatg ctgccatcaa ggcatttatt gcagtgtact atttgcttcc 360
aaaggatcag gggatcaccc tgagaaagct ggtacggggc gccaccctgg acatcgtgga 420
tggcatggct cagctcatgg aagtactttc cgtcactcca actcagagcc ctgagaacaa 480
tgaccttatt tcctacaaca gtgtctgggt tgcgtgccag cagatgcctc agataccaag 540
agataacaaa gctgcagctc ttttgatgct gaccaagaat gtggattttg tgaaggatgc 600
acatgaagaa atggagcagg ctgtggaaga atgtgaccct tactctggcc tcttgaatga 660
tactgaggag aacaactctg acaaccacaa tcatgaggat gatgtgttgg ggtttcccag 720
caatcaggac ttgtattggt cagaggacga tcaagagctc ataatcccat gccttgcgct 780
ggtgagagca tccaaagcct gcctgaagaa aattcggatg ttagtggcag agaatgggaa 840
gaaggatcag gtggcacagc tggatgacat tgtggatatt tctgatgaaa tcagccctag 900
tgtggatgat ttggctctga gcatatatcc acctatgtgt cacctgaccg tgcgaatcaa 960
ttctgcgaaa cttgtatctg ttttaaagaa ggcacttgaa attacaaaag caagtcatgt 1020
gacccctcag ccagaagata gttggatccc tttacttatt aatgccattg atcattgcat 1080
gaatagaatc aaggagctca ctcagagtga acttgaatta tgacttttca ggctcatttg 1140
tactctcttc ccctctcatc gtcatggtca ggctctgata cctgctttta aaatggagct 1200
agaatgcttg ctggattgaa agggagtgcc tatctatatt tagcaagaga cactattacc 1260
aaagattgtt ggttaggcca gattgacacc tatttataaa ccatatgcgt atatttttct 1320
gtgctatata tgaaaaataa ttgcatgatt tctcattcct gagtcatttc tcagagattc 1380
ctaggaaagc tgccttattc tctttttgca gtaaagtatg ttgttttcat tgtaaagatg 1440
ttgatggtct caataaaatg ctaacttgcc agtgattaaa aaaaaaaaaa 1490
<210>40
<211>1677
<212> DNA
<213>人
<400>40
cttgacccta tttatagtgg ctctaaaggt ggtgttatta tgttttctag agcacttcga 60
ttatacaaac gtcaaggaat ccgagttaat gtgctttgcc ctgagtttgt tgaaacagac 120
atgggcacaa tgatcggtcc caaattcctt agtatgatgg ggggctttgt acctatggaa 180
atggtggtga aaggtgcttt tgagctcatc actgatgaga ataaagccgg cgattgccta 240
tggattacta atcggcgagg tcttgagtac tggcccaccc catcagaaga agcaaagtac 300
ttgctgcgtt ctacacgttc caggagaaga actgaataca aagctccacc aattaaacta 360
cctgagagtt ttgagaaaat agttgttcag accttgactc acaactttcg gaatgctacc 420
agtgtagtaa gagcaccact gagattacct atcaaaccaa actatgttct tgtgaagata 480
atctatgctg gtgtaaatgc tagtgatgta aattttagct caggtcgcta ttttggtggc 540
aataacagtg acactgcatc ccgtcttccg tttgatgcag gatttgaggc tgtgggagta 600
attgcagcag ttggggattc tgttactgac ttgaaagttg gcatgccttg tgcgttcatg 660
acttttggag gctatgctga atttacaatg attccttcga aatacgccct tccaatgcct 720
agaccagaac cggaaggtgt tgccatgctt acatcaggat taacagcttc aattgctcta 780
gaaaaggcag gacagatgga atctggaaaa gtggtccttg ttactgctgc ggcaggagga 840
actggtcagt ttgctgttca gcttgcaaaa ttagctggta ataccgtggt tgccacttgt 900
ggaggtgggg caaaggccaa gcttctgaaa gaattgggag tcgacagagt catagactat 960
cacagtgaag atataaaaac ggttctaagg aaagagttcc cgaaaggtat tgatatcatc 1020
tacgaatctg ttggtgggga catgttaaag ttgtgcttgg atgctttggc agtccatgga 1080
cgactcattg tcattggcat gatttctcag tatcaaggag aaaatggttg gacgccatca 1140
aaatatcctg gactatgtga gaagctcttg tcaaagagtc aaactgtggc tggctttttc 1200
ctggtgcaat atagtcacat gtaccaagaa caccttaaca agttatttga cctttactct 1260
tccggaaaac taaaggttgc tgtggatcca aagagattta taggccttca ttctgttgct 1320
gatgctgttg agtatctcca ttcaggcaaa agcgttggga aggtggttgt ctgcgtggac 1380
ccgaccttcg gtcatcaagt agccaaatta tgaatgaaca cggtgtcaaa tacagaaaga 1440
agtgaagttt tcaattctta gtctagagat tgttctcgaa tgttactgaa aatagctgct 1500
agaccagtgc tggaatattt attctcaatg ctttttcaat tttggattac ttgaaagaat 1560
aatccattta tgtataccat gtttatgttt acactataca acaactatga gcagaagaaa 1620
gcgagatatc tacaaaataa attataatcc tttcatttta aaaaaaaaaa aaaaaaa 1677
<210>41
<211>1330
<212>DNA
<213>人
<400>41
atggcgcagt gggacagctt cactgatcaa caggaggaca ctgatagctg ttcagaatct 60
gtgaagtttg atgctcgctc caatacagct ttgcttcccc caaatcctaa aaatggccct 120
ccacttcaag aaaagctgaa atccttcaaa gctgcactga ttgcccttta tctccttgtg 180
tttgctgttc tcattcctat catcgcaata atggcagctc aactcctgaa gtgggaaatg 240
aagaattgca cagttggttc aattaatgca aacagtgtat cctccagtct cctgggaaga 300
ggaaatgaca gtgaagatga agtgagattt cgagaagttg ttatggaaca cattagcaag 360
atggagaaaa gaatccaata tatttcagat actgaagaaa atctcgtaga ttcagagcat 420
tttcaaaatt tcagtgtgac aactgatcaa cgatttgctg atgttcttct ccaactaagt 480
accttggttc ccacagtcca gggacatggg aatgccgtag atgaaatcac caggtcctta 540
ataagtctga ataccacgct gcttgatttg cacctctatg tagaaacact gaatgtcaaa 600
ttccaggaga atacacttaa agggcaagag gaaatcagca aattaaagga gcgtgtgcac 660
aatgcatcag cagaaattat gtctatgaaa gaagaacaag tgcatttgga acaggaaata 720
aaaagagaag tgaaagtcct gaataacatc actaatgatc tcaggctgaa agattgggaa 780
cattctcaga cgttgagaaa tatcacttta attcaaggtc ctcctggacc cccaggagaa 840
aaaggagata gaggtccaac cggagaaagt ggtccaccag gcgtttcagg tccagtaggt 900
cctccaggtc ttaagggtga tcgaggatct attggctttc cgggaagtcg aggatatcca 960
ggacaatcag ggaagactgg gaggacagga tatcctggac caaaaggcca aaagggagaa 1020
aaaggcagtg gaagcatcct gactccttct gcgactgtcc gactggttgg tggccgtggc 1080
cctcatgagg gtagagtgga gatattgcac aatggacagt ggggcacagt ttgtgatgat 1140
cactgggaac tgcgtgccgg gcaggttgtc tgcaggagct tgggataccg aggtgttaag 1200
agtgtgcaca agaaagctta ttttggacaa ggtactggtc ccatttggct gaatgaagta 1260
ccctgtttgg ggatggagtc atccattgaa gagtgcaaaa tcagacagtg gggcgtgaga 1320
gtctgttcac 1330
<210>42
<211>431
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(97)..(97)
<223>任意碱基
<220>
<221>misc_特征
<222>(347)..(347)
<223>任意碱基
<220>
<221>misc_特征
<222>(349)..(349)
<223>任意碱基
<220>
<221>misc_特征
<222>(361)..(361)
<223>任意碱基
<220>
<221>misc_特征
<222>(362)..(362)
<223>任意碱基
<220>
<221>misc_特征
<222>(363)..(363)
<223>任意碱基
<220>
<221>misc_特征
<222>(401)..(401)
<223>任意碱基
<220>
<221>misc_特征
<222>(428)..(428)
<223>任意碱基
<400>42
ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60
ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120
gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180
tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240
ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300
ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360
nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420
tcagctcnct g 431
<210>43
<211>669
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(641)..(641)
<223>任意碱基
<400>43
ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60
acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120
gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180
ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240
tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300
ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360
acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420
cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480
tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540
tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600
acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660
aatgaaact 669
<210>44
<211>287
<212>DNA
<213>人
<400>44
gccggagagt ctacaatgtt acccagcatg ctgttggcat tgttgtaaac aaacaagtta 60
agggcaagat tcttgccaag agaattaatg tgcgtattga gcacattaag cactctaaga 120
gccgagatag cttcctgaaa cgtgtgaagg aaaatgatca gaaaaagaaa gaagccaaag 180
agaaaggtac ctgggttcaa ctaaagcgcc acgctgctcc acccagagaa gcacactttg 240
tgagaaccaa tgggaaggag cctgagctgc tggaacctat tccctat 287
<210>45
<211>383
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(147)..(147)
<223>任意碱基
<220>
<221>misc_特征
<222>(309)..(309)
<223>任意碱基
<220>
<221>misc_特征
<222>(349)..(349)
<223>任意碱基
<220>
<221>misc_特征
<222>(365)..(365)
<223>任意碱基
<220>
<221>misc_特征
<222>(372)..(372)
<223>任意碱基
<220>
<221>misc_特征
<222>(380)..(380)
<223>任意碱基
<400>45
ggaacggaaa aggagaattc aagtgtgacc ctcatgaggc aacgtgttat gatgatggga 60
agacatacca cgtaggagaa cagtggcaga aggaatatct cggtgccatt tgctcctgca 120
catgctttgg aggccagcgg ggctcgnctt gtgacaactg ccgcagacct ggggggtgaa 180
cccagtcccg aaggcactac tggccagtcc tacaaccagt attcttcaga gataccattc 240
agagaacaaa cactaatgtt taatttgccc aatttgagtg cttcatgcct tttaggatgt 300
tacaggctng acagagaagg ttttcccgag gagttaaatc atctttttnc catttcccga 360
ggggncaagg cntgtttttn ttt 383
<210>46
<211>523
<212>DNA
<213>人
<400>46
cagaggggca gggcggacgg ctaggagttc aagaaacatc ctggtctgag ggaaaggctg 60
cagctgcacc gccatgaata agcttttcag cttctggaag aggaagaatg agacccgcag 120
ccagggctac aaccttcgag aaaaggattt aaagaaactt cacagagctg cttcagtcgg 180
ggatttgaag aagctgaagg aataccttca gatcaagaaa tatgatgtaa atatgcagga 240
ctatgaatac agaacacctt tgcacctagc ctgtgctaat ggacatacag atgttgtact 300
tttcctaatt gagcaacaat gcaagataaa tgtccgggat agtgaaaaca aatccccatt 360
gattaaggca gtacagtgtc aaaatgagga ttgtgctact attctgctaa actttggtgc 420
agacccagat ctgagggata ttcgttataa tactgttctt cactatgctg tttgtggtca 480
aagtttgtca ttagttgaaa aactgcttga atacgaagct gat 523
<210>47
<211>390
<212>DNA
<213>人
<400>47
tccaaggtca tggcaaaaca tctgaagttc atcgccagga ctgtgatggt acaggaaggg 60
aacgtggaaa gcgcatacag gaccctaaac agaatcctca ctatggatgg gctcattgag 120
gacattaagc atcggcggta ttatgagaag ccatgccgcc gcgacagagg gaaagctatg 180
aaaggtgccg gcggatctac aacatggaaa tggctcgcaa gatcaacttc ttgatgcgaa 240
agaatcgggc agatccgtgg cagggctgct gaggcctgtg ggtgggacac cagtgcgaaa 300
ccctcatcca gttttctctc catctctttt ctttgtacaa tcccatttcc tattaccatt 360
ctctgcaata aactcaaatc acatgtctgc 390
<210>48
<211>669
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(641)..(641)
<223>任意碱基
<400>48
ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60
acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120
gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180
ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240
tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300
ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360
acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420
cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480
tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540
tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600
acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660
aatgaaact 669
<210>49
<211>431
<212>DNA
<213>人
<220>
<221>misc_特征
<222>(97)..(97)
<223>任意碱基
<220>
<221>misc_特征
<222>(347)..(347)
<223>任意碱基
<220>
<221>misc_特征
<222>(349)..(349)
<223>任意碱基
<220>
<221>misc_特征
<222>(361)..(361)
<223>任意碱基
<220>
<221>misc_特征
<222>(362)..(362)
<223>任意碱基
<220>
<221>misc_特征
<222>(363)..(363)
<223>任意碱基
<220>
<221>misc_特征
<222>(401)..(401)
<223>任意碱基
<220>
<221>misc_特征
<222>(428)..(428)
<223>任意碱基
<400>49
ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60
ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120
gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180
tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240
ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300
ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360
nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420
tcagctcnct g 431
Claims (18)
1.一种评估结肠直肠癌状况的方法，包括在选自Seq.ID.No.42-49的基因组合中鉴定每个基因(相对于正常种群的相同基因的表达)的差异调节。
2.权利要求1的方法，其中调节基因表达中存在至少2倍差异。
3.权利要求1的方法，其中表示差异调节的p值低于.05。
4.权利要求1的方法，进一步包括用于非遗传基础的结肠直肠癌诊断。
5.权利要求的方法，其中非遗传基础的癌症标记选自癌胚性抗原、CA19-9、CA125、CK-BB和鸟苷酸环化酶C。
6.一种诊断框架，包括选自Seq.ID.No.42-49的基因组合中分离的核酸序列、其互补序列或其一部分序列。
7.位于基片上的如权利要求6的诊断框架，其中所述的基片适合鉴定包含于其上的基因差异表达。
8.权利要求7的诊断框架，其中所述的基片用于微阵列。
9.权利要求8的诊断框架，其中所述的微阵列是cDNA微阵列。
10.权利要求8的诊断框架，其中所述的微阵列是寡核苷酸微阵列。
11.一种诊断框架，包括选自Seq.ID.No.42-49的基因组合中分离的核酸序列、其互补序列或其一部分序列。
12.一种用于诊断结肠直肠癌的试剂盒，包括选自Seq.ID.No.42-49的基因组合中分离的核酸序列、其互补序列或其一部分序列。
13.权利要求12的试剂盒，进一步包括进行微阵列分析的试剂。
14.权利要求12的试剂盒，进一步包括媒介物，所述的核酸序列、其互补序列或其部分序列通过该媒介物进行检测。
15.一种评估结肠直肠癌疗效的方法，包括选自Seq.ID.No.42-49的基因相结合，鉴定每个基因(相对于正常种群的相同基因的表达)的差异调节。
16.权利要求15的方法，其中对疗效的评估包括确定患者是否好转，非好转，复发，可能好转或可能复发。
17.评估结肠直肠癌状况的制品，包括选自Seq.ID.No.42-49的基因组合中分离的核酸序列、其互补序列或其一部分序列。
18.评估结肠直肠癌状况的制品，包括选自Seq.ID.No.42-49的基因组合中分离的核酸序列、其互补序列或其一部分序列的表示物。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586420A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测乳腺癌易感基因的方法及试剂盒 |
| CN102586423A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测结直肠癌易感基因的方法及试剂盒 |
| CN110054692A (zh) * | 2009-10-23 | 2019-07-26 | 米伦纽姆医药公司 | 抗gcc抗体分子及其相关组合物和方法 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4517189B2 (ja) * | 2003-05-19 | 2010-08-04 | 生化学工業株式会社 | 糖ヌクレオチド運搬作用を有するタンパク質、組織の癌化の検出方法 |
| MX2008011356A (es) * | 2006-03-03 | 2008-09-15 | Veridex Llc | Prueba molecular para predecir la recurrencia del cancer de colon de la etapa b de duke. |
| ES2332557B1 (es) * | 2007-12-04 | 2011-02-02 | Universidad Autonoma De Madrid | Huella genomica para el pronostico de la evolucion de adenocarcinoma colorectal. |
| US20120172244A1 (en) | 2010-12-20 | 2012-07-05 | Steven Buechler | Biomarkers and uses thereof in prognosis and treatment strategies for right-side colon cancer disease and left-side colon cancer disease |
| WO2012088146A2 (en) * | 2010-12-20 | 2012-06-28 | The University Of Notre Dame | Biomarkers and uses thereof in prognosis and treatment strategies for right-side colon cancer disease and left-side colon cancer disease |
| CN106367527A (zh) * | 2016-11-17 | 2017-02-01 | 苏州大学附属第二医院 | 直肠癌放化疗疗效相关靶基因的鉴定 |
| CN112831563A (zh) * | 2021-02-08 | 2021-05-25 | 复旦大学附属肿瘤医院 | 一种遗传性结直肠癌的检测panel及检测试剂盒 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5700637A (en) * | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| GB8822228D0 (en) * | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
| US5242974A (en) * | 1991-11-22 | 1993-09-07 | Affymax Technologies N.V. | Polymer reversal on solid surfaces |
| US5527681A (en) * | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
| US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| DE3924454A1 (de) * | 1989-07-24 | 1991-02-07 | Cornelis P Prof Dr Hollenberg | Die anwendung von dna und dna-technologie fuer die konstruktion von netzwerken zur verwendung in der chip-konstruktion und chip-produktion (dna chips) |
| IL103674A0 (en) * | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
| US5384261A (en) * | 1991-11-22 | 1995-01-24 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis using mechanically directed flow paths |
| US5412087A (en) * | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
| US5554501A (en) * | 1992-10-29 | 1996-09-10 | Beckman Instruments, Inc. | Biopolymer synthesis using surface activated biaxially oriented polypropylene |
| US5472672A (en) * | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
| US5429807A (en) * | 1993-10-28 | 1995-07-04 | Beckman Instruments, Inc. | Method and apparatus for creating biopolymer arrays on a solid support surface |
| US5571639A (en) * | 1994-05-24 | 1996-11-05 | Affymax Technologies N.V. | Computer-aided engineering system for design of sequence arrays and lithographic masks |
| US5556752A (en) * | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
| US6350576B1 (en) * | 1994-12-20 | 2002-02-26 | Cold Spring Harbor Laboratory | Cancer detection probes |
| US5599695A (en) * | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
| US5624711A (en) * | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
| US5545531A (en) * | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
| US5658734A (en) * | 1995-10-17 | 1997-08-19 | International Business Machines Corporation | Process for synthesizing chemical compounds |
| US6275814B1 (en) * | 1996-11-27 | 2001-08-14 | Investment Strategies Network | Investment portfolio selection system and method |
| US6003018A (en) * | 1998-03-27 | 1999-12-14 | Michaud Partners Llp | Portfolio optimization by means of resampled efficient frontiers |
| US6218114B1 (en) * | 1998-03-27 | 2001-04-17 | Academia Sinica | Methods for detecting differentially expressed genes |
| US6004755A (en) * | 1998-04-07 | 1999-12-21 | Incyte Pharmaceuticals, Inc. | Quantitative microarray hybridizaton assays |
| US6218122B1 (en) * | 1998-06-19 | 2001-04-17 | Rosetta Inpharmatics, Inc. | Methods of monitoring disease states and therapies using gene expression profiles |
| WO2000012702A2 (en) * | 1998-08-31 | 2000-03-09 | Bayer Corporation | Human genes differentially expressed in colorectal cancer |
| US6175824B1 (en) * | 1999-07-14 | 2001-01-16 | Chi Research, Inc. | Method and apparatus for choosing a stock portfolio, based on patent indicators |
| EP1196027A4 (en) * | 1999-07-15 | 2004-12-22 | Res Dev Foundation | CORTICOTROPIN RELEASE FACTOR-2 RECEPTOR 2 MOUSE AND USES THEREOF |
| US6271002B1 (en) * | 1999-10-04 | 2001-08-07 | Rosetta Inpharmatics, Inc. | RNA amplification method |
-
2003
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110054692A (zh) * | 2009-10-23 | 2019-07-26 | 米伦纽姆医药公司 | 抗gcc抗体分子及其相关组合物和方法 |
| CN102586420A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测乳腺癌易感基因的方法及试剂盒 |
| CN102586423A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测结直肠癌易感基因的方法及试剂盒 |
Also Published As
| Publication number | Publication date |
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| US20030186302A1 (en) | 2003-10-02 |
| JP4354724B2 (ja) | 2009-10-28 |
| CA2422298C (en) | 2013-07-30 |
| EP1355149A2 (en) | 2003-10-22 |
| AU2003203562A1 (en) | 2003-10-23 |
| KR20030078802A (ko) | 2003-10-08 |
| CN101603092A (zh) | 2009-12-16 |
| EP1355149A3 (en) | 2004-08-25 |
| BR0303091A (pt) | 2004-02-17 |
| CN101603092B (zh) | 2012-11-07 |
| AR039617A1 (es) | 2005-03-02 |
| KR100991673B1 (ko) | 2010-11-04 |
| JP2003325190A (ja) | 2003-11-18 |
| JP2009153522A (ja) | 2009-07-16 |
| CA2422298A1 (en) | 2003-09-29 |
| CN100516233C (zh) | 2009-07-22 |
| MXPA03002866A (es) | 2004-08-11 |
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