CN1502989A - 测定结肠癌 - Google Patents
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- CN1502989A CN1502989A CNA031378714A CN03137871A CN1502989A CN 1502989 A CN1502989 A CN 1502989A CN A031378714 A CNA031378714 A CN A031378714A CN 03137871 A CN03137871 A CN 03137871A CN 1502989 A CN1502989 A CN 1502989A
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Abstract
通过分析一组基因的表达在相信患有结肠癌的人中评估结肠癌的存在与否或存在的可能性的方法。在各种介质(如微阵列)中的基因表达组合体，以及包含它们的试剂盒。
Description
背景技术
本申请要求2002年3月29日递交的美国临时申请No.60/368,687的利益。
本发明涉及基于生物样品的基因表达方案的结肠癌的诊断和预后。
结肠癌是一种异源疾病，包括被认为通过3个主要的分子机制发生的肿瘤：1)腺瘤结肠息肉病(APC)基因，或β-连环蛋白基因中与染色体的不稳定性相关的突变，2)在DNA错配修复基因如MLH1、MSH2、PMS1、PMS2和MSH6中的与微卫星不稳定性相关的突变，和含有短的重复序列的基因中的突变，和3)肿瘤阻遏基因的启动子区中的超甲基化诱导的基因沉默。个别的结肠癌的遗传互补似乎是包括遗传不稳定性、特异的突变和基因沉默的不同的结合。通常染色体的不稳定性(CIN)是癌症的共同特征。暗示了一个非整倍体表现型，其中整个的染色体或大部分的染色体丢失或重新获得了。微体的不稳定性(MIN)是在二倍体肿瘤中发现的，在短的重复序列中突变的速度有增加。遗传的不稳定性的两种形式是结肠癌中共同的。
所以，结肠癌具有复杂的起源，并且包括在许多不同的生物途径中的许多相互的作用。在组织学中，用于辅助提供诊断、预后或治疗检测决定的血清标记、组织学和细胞学检测经常不具有需要的可靠性。同样，当利用单个遗传标记(例如，提高特定基因的表达)可能是有用的，而由于癌症的多样性更可能的是，遗传标记组合体(portfolio)是最好的途径。
发明概述
本发明是评估结肠癌的存在与否，或确信有结肠癌的人的可能的症状的方法。在本方法中，对病人样品的基因表达的方案据分析可以确定是否病人具有结肠癌，是否病人不患有结肠癌，是否病人可能会患有结肠癌，或对待治疗结肠癌的病人的治疗的应答。
用于实施本方法的材料(article)也是本发明的一个方面。这样的材料包括基因表达的分布图，或它们固定于机器可读介质如计算机可读介质中的表示。
用于鉴定基因表达分布图(profile)的材料也可以包括底物或表面，如微阵列，以捕获和/或指明基因表达的存在、缺乏或程度。
发明详述
在组织样品中存在很少或没有特定核酸序列只有很少被发现具有诊断或预后价值。关于各种蛋白质、肽或mRNA的表达的信息在另一方面渐渐地被重视。在基因组本身中具有表达蛋白质、肽或mRNA(如称为“基因”的这样的序列)的很少存在的核酸序列是不能确定是否给定的细胞中表达了蛋白质、肽或mRNA。能够表达蛋白质、肽或mRNA的给定的基因是否是这样的，并且表达进行到何种程度，是由各种复杂的因子确定的。不考虑理解和评估这些因子的困难，测定基因表达可以提供关于重要的事件的出现如肿瘤的发生、转移、程序死亡和其它临床相关现象的有用的信息。可以在基因表达的分布图中发现基因是有活性或无活性的程度的相关指示。本发明的基因表达分布图可用于诊断和治疗结肠癌患者。
样品的制备需要收集患者的样品。用于本发明的方法的患者的样品是怀疑含有疾病细胞(如从结肠样品或外科手术范围获取的上皮细胞)的那些。获得可疑的样品的一个有用的技术是激光捕获显微解剖术(LCM)。LCM技术提供了选择待研究的细胞的方法，使细胞类型异质性引起的可变性最小。结果，可以容易地检测正常和癌性细胞之间的基因表达中的适度的或小的变化。在优选的方法中，这些样品中含有从周边血液提取的循环的上皮细胞。这些可以根据许多方法来获得，但最优选的方法是授予Immunivest公司的美国专利6,136,182中所述的磁性分离技术，该专利引入本文作为参考。一旦已经得到含有需要的细胞的样品，对于适当的方式中的基因，优选地通过微阵列提取和扩增RNA，得到了基因表达方案。
确定基因表达方式的优选方法包括确定可以编码蛋白质或肽的基因产生的RNA的量。这是通过逆转录酶PCR(RT-PCR)，竞争性RT-PCR，实时RT-PCR，差示RT-PCR，Northern印迹分析和其它相关的测试完成的。当利用单独PCR反应实施这些技术是可能的时，扩增从mRNA产生的互补DNA(cDNA)或互补RNA(cRNA)并且通过微阵列分析是最好的。许多不同的阵列构型和他们的生产方法是本领域技术人员已知的，并且在下面的美国专利中有叙述：5,445,934；5,532,128；5,556,752；5,242,974；5,384,261；5,405,783；5,412,087；5,424,186；5,429,807；5,436,327；5,472,672；5,527,681；5,529,756；5,545,531；5,554,501；5,561,071；5,571,639；5,593,839；5,599,695；5,624,711；5,658,734和5,700,637；这些文献引入本文作为参考。
微阵列技术允许同时测量几千个基因的稳定态mRNA水平，因此是一个用于鉴定如未控制的细胞增殖的启动、捕获或调节等效果的有效工具。两个微阵列技术是目前广泛使用的。第一个是cDNA阵列，第二个是寡聚核苷酸阵列。虽然在这些芯片的构建中存在差异，基本上，所有下游的数据分析和结果是相同。这些分析的产物通常是对标记探针发出的信号的强度的测量，用于检测在微阵列的已知位置与核酸序列杂交的样品的cDNA序列。通常，信号的强度与cDNA，和由此在样品细胞中表达的mRNA的量成比例。许多这样的技术是可得的和有用的。优选的检测基因表达的方法可以在下面的专利中找到，如授予Linsley等人的美国专利6,271,002；授予Friend等人的6,218,122；授予Peck等人的6,218,114；和授予Wang.，等人的6,004,755，这些公开物引入本文作为参考。
通过比较这样的强度对表达水平进行分析。这最好通过产生一个测试样品和对照样品中的基因的表达强度的比例矩阵来进行。例如，来自疾病组织的基因表达强度可以与相同类型的正常组织产生的表达强度比较(例如，有病的结肠组织样品与正常的结肠组织样品比较)。这些表达强度的比例表明了在测试样品和对照样品之间的基因表达成倍地变化。
基因表达方式也可以许多方法表示。最常见的方法是粗(raw)荧光强度或比例矩阵排成树状图谱，其中的纵列表示测试样品，横行表示基因。这样安排数据使具有相似的表达方式的基因相互邻近。每个基因的表达比例是表示成一个颜色。例如，小于1的比例(表示下调)可以表示在图谱的蓝色部分，而大于1的比例(表示上调)可以表示成图谱的红色部分的颜色。商业可得的计算机软件程序可用于表示这样的数据，包括“GENESPRINT”，来自Silicon Genetics，Inc.，和“DISCOVERY”和“INFER”，来自Partek，Inc.的软件。
用于本发明的方法的调节基因表示在表1中。表达有差异的基因表示为在有病细胞中上调或下调。上调和下调是相对的术语，指在一些基线相关的基因的表达的量中发现了可检测的不同(在用于测量它的系统中的噪音的分布之)。在这种情况中，基线是正常细胞的已测量的基因表达。然后利用相同的实验方法，在有病的细胞中的需要的基因对照基线水平上调或下调。在本文中，有病是指打断或打乱，或具有打乱身体功能的适当效能的身体的状态的变化，如细胞的未受控制的增殖所发生的。当人的基因型或表现型与疾病的存在一致时，一些人被诊断有病。但是，进行诊断或预后的行为包括确定疾病/状态组织如治疗性检测。在治疗性检测中，通过比较一个时段的基因的表达确定是否基因表达方式改变或正在改变成与正常组织更一致的方式对给定的治疗方法进行临床判断。
优选地，根据杂交的微阵列探针的强度测量的成倍的变化可以区别上调和下调的水平。产生这样的差别或小于.05的p值，2.0倍的差异是优选的。所以，在说有病和正常细胞中基因的表达不同之前，发现疾病细胞比正常细胞至少产生了2倍多，或2倍以下的强度。差异倍数越大，利用该基因作为诊断越优选。选择本发明的基因表达的方式的基因具有的表达水平能导致产生将正常的或非调节基因区分开的信号，其量可用临床实验仪器可检测的超过背景值。
统计数值可以用于区别调节的和非调节的基因和噪音。统计实验发现，在不同的样品组之间基因的差别最明显。Student的t-实验是有说服力的统计测试的例子，可以用于发现两个组之间的明显的差异。P-值越低，基因在展示不同组之间的差异的证据更加可信。然而，因为微阵列测量一次有一个以上的基因，一次可以进行上万个统计实验。因为这样，似乎偶然看到小的p-值，并且可以进行Sidak校正实验以及任意化/变换实验来调整。T-测试的p值小于0.05是该基因明显不同的证据。更不得不相信的证据是在Sidak校正实验后，p值小于0.05。在每个组的大量样品，在随机化/变换测试后，小于0.05的p-值是最值得相信的差异明显的证据。
可以用于选择产生信号的基因的另一个参数大于非调节基因或噪声，是利用绝对信号的差异的测量方法。优选地，已调节的基因表达产生的信号至少比正常或非调节的基因的有20％的不同(在绝对值的基础上)。甚至更优选地，这样的基因产生的表达方式与正常或非调节基因的至少有30％的不同。
基因可以分成小组，使该组中得到的基因系列的信息提供了进行临床相关判断如诊断，预后或治疗选择的正确基础。这些系列的基因组成了本发明的模式。在这种情况中，该模式支持的判断包括结肠癌。基因表达方式的组合体可以组成实施例3中叙述的基因的组合。如大多数诊断标记，利用足以进行准确的医学判断的最少数目的标记通常通常是令人满意的。这防止了治疗的进一步的分析的延误以及对时间和资源的不恰当的利用。在这种情况中，这样的最小组合体可以组成实施例4的基因的组合。
优选地，建立组合体使组合体中的基因的组合与个体的基因或随机选择的基因的组合相比，具有的敏感性和特异性提高。在本发明中，组合体的敏感性可以反映在疾病状态和正常状态中基因表达显示的成倍的差异。特异性可以反映在基因表达的信号与感兴趣的症状的相关性的统计测量中。例如，标准偏差可以用于这样的测定。在考虑组合体中包括的一个基因组时，在表达测量中的一个小标准的偏差与更大的特异性相关。也可以利用其它的变化程度的测定如相关系数。
确定基因表达组合体的最优选的方法是通过最佳化算式的利用，如广泛用于建立的储存组合体中的平均变化算式。该方法在相同日期的Tim Jatkoe等人的题目为“组合体选择”的在审专利申请中有详细叙述。基本上，该方法要求建立一系列的输入(在财务应用中存储，表达为本文的强度的测量值)，将使能接受的输出量最佳(例如，产生的信号)，在输出量的变化最小时接收以便利用。许多商业软件程序可以获得用于进行这样的操作。“Wagner相关平均变化最佳应用”指本申请说明书的“Wagner软件″是优选的。这一软件利用了来自“Wagner相关平均变化最佳文库”的功能确定有效的前沿，在Markowitz意义中的最佳组合体是优选的。
这一类型的软件的利用需要待转化的微阵列数据，使它可以存储输出量的方式处理为输入数据，当软件用于它打算应用的财务分析目的时，利用了危险测量。例如，当将Wagner软件与微阵列强度测量结合利用时，可以利用下面的数据转化方法。
基因首先是通过鉴定那些基因来预选的，这些基因的表达显示至少有一些最小水平的差异。如下进行优选的预选方法。选择一个基准类别。通常，这将包括来自不具有感兴趣的病症的群体的基因。例如，如果一个人有兴趣选择一个诊断乳腺癌的基因的组合体，来自没有乳腺癌的患者的样品可以用作基准类别。一旦选择了基准类别，计算基准类别样品的每个基因的基因表达的指示剂的算术平均值和标准偏差。这一指示剂通常是微阵列读数的荧光强度。然后，将计算的统计数据用于计算每个基因的基准值(X*标准偏差+平均值)。这是该基因的基准读数，由此比较所有其它样品。X是配制组合体的人选择的可变的严格度。X的值越高，严格度越高。优选地，X在0.5到3的范围，更优选地2到3，3是最优选的。
然后计算每个实验样品(具有感兴趣的病症的那些)与基准读数之间的比例。然后，为了容易用软件操作数据，将比例转化成基准10的对数值。根据Markman平均变化算法，利用Wagner软件，这样可以使下调基因具有最佳化需要的负值。
具有这些转化比例的预处理数据可以用作输入值，取代通常用于财务分析目的的Wagner软件的财产输出值。
一旦形成有效的前沿，就选择对应于前沿点的给定的输入水平(输出量)或变化的最佳组合体。这些输入或变化是制作组合体的人设定的预定标准。换句话说，寻找最佳的组合体的人确定可接受的输入水平(指示了敏感度)或给定水平的变化(指示了特异性)并且选择了位于对应于输入水平或变化的有效前沿的基因。当选择输入水平或变化时，Wagner软件可以选择这样的基因。为了在存储组合体中存储，也可以在组合体中对每个基因指定一个重量。
通过比较患者样品的组合体中基因的表达与用于建立组合体的不同表达的基因的计算值可以确定是否样品具有组合体诊断的病症。优选地，通过在组合体选择过程中指定该基因的重量，统计组合体中的每个基因的强度值的倍数首先产生了组合体值。然后，根据(Y*标准偏差+基准组的组合体值的平均值)计算边界值，其中Y是具有与上述X相同的平均值的严格度值。然后，将具有比基准类别的组合体值更大的组合体值的样品划分为具有该症状。如果需要，这一方法可以根据已知的统计方法重复进行，用于提高可信水平。
或者，可以重复这一方法直到达到预定的准确率。
组合体的选择的方法和一个未知的鉴定概括如下：
1.选择基准类别
2.计算基准类别样品的每个基因的平均值，和标准偏差
3.计算每个基因的(X*标准偏差+平均)。这是将比较的所有其它样品的基准读数。X是可变的严格度，X值越高，严格度越高。
4.在步骤3中计算每个实验样品和计算的基准读数之间的比例。
5.转化比例，使这些比例小于1，是负数(例如，利用对数基准10)。
(负调节的基因现在正确地具有MV最佳化所需要的负值)
6.这些转化比例用作输入，取代通常用于软件应用的财产输出值。
7.该软件将划出有效的前沿，并且沿着有效前沿在任何点输出一个最佳的组合体。
8.在有效前沿上选择需要的输出值或方差。
9.通过组合体选择算式统计产生的每个基因的强度值(以重量表示)的倍数计算每个样品的组合体值。
10.在Y的倍数和基准组合体值的标准偏差上增加基准组的平均组合体值计算边界值。比这一边界值大的值应该划分为实验类别。
11.选择性地可以重复这一过程直到获得最好的预测准确度。
或者，首先，通过鉴定那些表达显示一些最小分化水平的基因来预选择基因。在备选的方法中的预选择优选地是基于
给出的阈值，其中μt是已知具有疾病或症状的亚系的平均值，μn是正常的样品的亚系的平均值，δt+δn代表合并的标准偏差。根据例如
的关系预选择数据也可以利用噪声截断的信号。这保证了根据不同调节预选择的基因是以临床意义的方式区别的。那是在适于测量诊断参数的任务的仪器的噪声水平以上的。对于根据这些规则预选择的每个标记，确定基数，其中纵列代表样品，横行代表标记，每个元素是一个标准化的强度测量值，用于根据
的关系式表达标记，其中I是强度测量值。
设置其它边界条件定义最佳组合体也是可能的。例如，组合体大小可以限制于固定的范围或标记的数目。这可以通过制定更严格的数据预选择规则(例如,
而不是
或利用程序特征如限制组合体大小来进行。例如，一个人可以设置边缘条件，从最适合的10个基因中选择有效的前沿。也可以利用所有预选择的基因，以确定有效前沿，然后限制选择的基因的数目(例如，不超过10个)。
选择组合体的方法也可以包括应用启发式规则。优选地，这样的规则是根据生物学和对用于产生临床结果的技术的理解来制定的。更优选地，它们应用于来自最佳化方法的结果。例如，组合体选择的平均变化方法可以用于许多在患有乳腺癌的患者中不同地表达的基因的微阵列数据。来自该方法的结果将是可以包括在周边血液以及有病的乳腺组织中表达的一些基因的最佳的基因系列。如果用于该测试方法的样品是从周边血液中得到的，在乳腺癌情况中不同地表达的一些基因也可以在周边血液中不同地表达，然后，可以利用推断规则，其中从有效前沿选择在周边血液中不同表达的以外的组合体。当然，该规则可以在形成有效前沿以前，例如通过在数据预选择过程中应用该规则来应用。
也可以应用其它推断规则，但不必定与所讨论的生物学相关。例如，可以应用此规则，仅有给定百分数的组合体可以特定的基因或许多基因为代表。购买到的软件如Wagner软件容易装备这些类型的直观推断。例如，当除了准确度和准确性以外的因素(例如，预算的许可费)对参与的一个或多个基因的满意度有影响时，这也是可用的。
本发明的一个方法包括比较各种基因的基因表达方案(或组合体)进行如上所述的诊断。包括每个基因的基因表达方式的组合体是固定于介质中的，如计算机可读介质中。这可以采取许多形式。例如，可以确定一个表格，其中输入疾病的信号指示的范围(例如，强度测量值)。然后，在表格中可以比较真正的病人资料和这些值确定是否病人的样品是正常的或有病的。在更复杂的实施方案中，表达信号的方式(例如，荧光强度)用数据或图谱来记录。然后，将与病人样品结合利用的基因组合体产生的基因表达方式与这些表达方式比较。然后，将方式比较软件用于确定是否病人的样品具有表示所讨论的疾病的方式。当然，这些比较也可以用于确定是否病人的结果是正常。然后，将样品的表达方式与正常或对照细胞的组合体比较。如果，该样品表达方式与结肠癌的表达方式一致(在没有补偿医学考虑的情况下)，那么病人被诊断为结肠癌阳性。如果样品的表达方式与来自正常或对照细胞的表达方式一致，那么病人被诊断为结肠癌阴性。
可以利用许多已知的识别方式的方法。下面的参考提供了一些例子：
重量表决：
Golub，TR.，Slonim，DK.，Tamaya，P.，Huard，C.，Gaasenbeek，M.，Mesirov，JP，Coller，H.，Loh，L，Downing，JR.，Caligiuri，MA.，Bloomfield，CD.，Lander，ES.，癌症的分子分类：基因表达检测的类别发现和类别预测。科学286：531-537，1999。
支持载体机器：
Su，AI.，Welsh，JB.，Sapinoso，LM.，Kern，SG.，Dimitrov，P.，Lapp，H.，Schultz，PG.，Powell，SM.，Moskaluk，CA.，Frierson，HF.Jr，Hampton，GM.利用基因表达特征对人癌症进行分子分类，癌症研究61：7388-93，2001。
Ramaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，CH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T.，Gerald，W.，Loda，M.，Lander，ES.，Gould，TR.利用肿瘤基因表达分类进行多类别的癌症诊断，Proceedings of the National Academy of Sciences of the USA 98：15 149-15154，2001。
K-最近的邻居：
Ramaswamy，S.，Tamayo，P.，Rifkin，R.，Mukherjee，S.，Yeang，CH.，Angelo，M.，Ladd，C.，Reich，M.，Latulippe，E.，Mesirov，JP.，Poggio，T，Gerald，W，Loda，M.，Lander，ES.，Gould，TR.利用肿瘤基因表达特征进行多类别的癌症诊断，Proceedings of the National Academy of Sciences ofthe USA 98：15149-15154，2001
相关系数：
van’t Veer LJ，Dai H，van de Vijver MJ，He YD，Hart AA，Mao M，Peterse HL，van der Kooy K，Marton MJ，Witteveen AT，Schreiber GJ，Kerkhoven RM，Roberts C，Linsley PS，Bernards R，Friend SH.基因表达方式预测乳腺癌的临床结果，Nature，2002年1月31日，415(6871)：531-6。
本发明的基因表达方案也可以与用于癌症诊断，预后，或治疗检测的其它非遗传诊断的方法结合使用。例如，在一些情况中，将如上所述的基于基因表达的方法的诊断效力和来自常规标记如血清蛋白质标记(例如，癌性胚胎抗原)的数据结合是有益的。存在这样的标记的范围包括下面的分析值，如CA19-9，CA125，CK-BB和Guanylyl环化酶C。在一个这样的方法中，血液是周期性地取自己治疗的病人，并且然后进行一个如上所述的血清标记的酶免疫测试。当标记的浓度表示了肿瘤的恢复或治疗的失败，获取样品源进行基因表达分析。当存在一个可疑的质量时，用细微的针吸，然后如上所述分析从该质量取的细胞的基因表达方式。或者，可以从已经移取肿瘤的组织的邻近区域取组织样品。在其它测试方法不明确时这一途径特别有用。
当其它诊断的可靠性有疑问的时候，使用遗传标记和其它诊断结合最优选。例如，已知，CEA的血清水平可能基本上受病人的癌症状态不相关的因子的影响。在治疗结肠癌后监测到的患者显示了更高水平的常规CEA实验，进行基因表达/CEA测试是有益的。
本发明的材料包括用于治疗，诊断，预后和其它评估疾病的基因表达方式的代表。这些组合体代表减少到了可以自动通过机器如计算机可读介质(磁性的，光学的等等)来阅读。材料也可以包括用于评估这样的介质中的基因表达组合体的使用说明。例如，这些材料可以包括具有计算机说明的CD ROM，用于比较如上所述的基因的组合体的基因表达方式。材料也可以具有其中数字阅读的基因表达方式，使它们可以与来自患者样品的基因表达数据比较。或者，表达方式可以在不同代表的格式下记录。图象记录是一个这样的格式。成簇的算式如在上面提到的“GENSPRING”和“DISCOVER”计算机程序中掺入的那些可以最好地辅助这些数据的观察。
本发明的不同类型的生产材料是用于发现基因表达方式的介质或格式化测试。例如，这些可以包括微阵列，其中序列补体或探针固定于基质上，指示感兴趣的基因的序列合并产生了它们的存在的可读决定簇。或者，本发明的材料可以做成试剂盒形式，进行杂交，扩增，和指示感兴趣的基因的表达水平的信号的产生，用于检测结肠癌。
根据本发明制成的试剂盒包括确定基因表达方式的格式化测试。这些可以包括进行测试需要的所有或一些物质例如试剂和指示。
本发明另外是通过下面的非限制性例子说明的。
实施例：参考基因库数据库中的Gene ID数目鉴定了本发明分析的基因。这些通常与编码蛋白质或肽的产生的全长核酸序列相关。本领域普通技术人员能认识到，鉴定全长的序列不是观察到的分析点所需要的。即，部分序列或EST可以根据已知的原则来选择，将探针设计成评估对应的基因的基因表达的。
实施例1：样品处理和LCM
从已经做了结肠肿瘤外科手术的患者中收集27个新鲜的冷冻组织样品。19个样品是结肠恶性样本，其中8个样品是正常的结肠粘膜。在液氮中冷冻组织一小会儿，在20-30分钟内收集，然后储存在-80℃。对于激光捕获，切割样品(6um)，在玻璃片上计数一个切片，在薄膜上(P.A.L.M)上计数第二个，第二个已经固定在玻璃片上(Micro Slides Colorfrost，VWR Scientific，Media，PA)。然后，将在玻璃片上计数的切片固定在冷的丙酮中，用Mayer Haematoxylin染色(Sigma，St.Louis，MO)。病理学家分析样品用于诊断和分级。从附带的外科病理学和临床报告，利用Dukes分类估计临床阶段。在薄膜上固定切片，然后在100％的乙醇中固定5分钟，在曙红/100％乙醇中染色1分钟(100ml的脱水乙醇中100ug的曙红)，再次在100％的乙醇中快速浸渍一次，除去游离的颜色，空气干燥10分钟。
结肠腺癌中的两个是1级的，10个是2级的，5个是3级的。一个恶性样品是盲肠的类癌肿瘤，一个是转移的黑素瘤病灶。两个腺癌样品代表了粘膜亚型，一个是指细胞亚型。腺癌的Dukes分期如下：Dukes A：2，Dukes B：5，Dukes C：7，Dukes D：3。腺癌的6个已经预先辐射治疗。
在LCM中使用之前，将膜(LPC-MEMBRANE PEN FOIL 1.35um No 8100，P.A.L.M.GmbH Mikrolaser技术公司，Bernried，德国)和薄片预处理去除RNA酶，并且加强了组织样品在薄膜上的附着作用。简要地说，在DEP水中洗涤玻片，然后在Rnase AWAY中洗涤薄膜(分子生物产品公司，San Diego，CA)，并且在DEP水中漂洗。在将薄膜附着于玻璃片上后，在+120℃，烘干8小时，用TI-SAD处理(诊断产品公司，Los Angeles，CA，1∶50洛于DEP水中，通过脱脂棉过滤)，并且在+37℃温育30分钟。在使用之前立即在薄膜上喷洒10ul的Rnase抑制剂溶液(Rnasin抑制剂2500U＝33U/uIN211A，Promega GmbH，Mannheim，德国，于400ul的冷冻溶液中0.5ul，溶液含有0.15mol NaCl，10mmolTris pH8.0，0.25mmol二硫苏糖醇)，其中对组织样品进行计数。
将在薄膜上计数的组织切片用于LCM。利用与Zeiss Axiovert 135显微镜(Carl Zeiss Jena GmbH，Jena，德国)结合的PALM Robot-Microbeam(P.A.L.M.Mikrolaser Technologie，Carl Zeiss，Inc.，Thomwood，NY)技术捕获大约2000个上皮细胞/样品。还包括在正常粘膜中的周围的基质和在癌症样品中偶然的干扰基质成分。将捕获的细胞放置于100％的乙醇的试管中，保存于-80℃。
实施例2：RNA提取和扩增
将Zymo-Spin柱(Zymo Reserch，Orange，CA 92867)用于从LCM捕获的样品提取总RNA。在10ul的水中再悬浮约2ng的总RNA，进行2轮基于T7 RNA聚合酶的扩增产生约50ng的已扩增的RNA。
实施例3：cDNA微阵列杂交和定量分析
利用组成约20,000个人cDNA克隆的一系列cDNA微阵列以测试样品。同时在微阵列上影印约30个植物基因作为非特异杂交的对照。从LCM捕获的细胞的5ug RNA合成Cy3标记的cDNA探针。用Qiagen核苷酸移取柱纯化探针，然后与微阵列杂交14-16小时。洗涤玻片，在扫描之前空气中干燥。扫描cDNA微阵列的cy3荧光，并且将ImaGene软件(Biodiscovery，Los Angeles，CA)用于定量分析。对于每个cDNA克隆，利用双份点和双份阵列获得四个测量值，取强度的平均值。用Generation III Micro-array Spotter(Molecular Dynamics)在氨基-硅烷包被的玻片(Corning)上印迹cDNA。PCR扩增，纯化(Qiagen PCR纯化试剂盒)cDNA，并且与10M NaSCN影印缓冲液1∶1混合。在杂交之前，在异丙醇中，室温下温育微阵列10分钟。在95℃温育探针2分钟，在室温下5分钟，然后加到3个重复的玻片上。用DPX(Fluka)，在玻片上封闭覆盖的玻片，并且在42℃温育过夜。然后，在55℃，在1×SSC/0.2％SDS和0.1×SSC/0.2％SDS中洗涤玻片5分钟，在0.1×SSC中浸渍，并且在用GenIII阵列扫描仪(MolecularDynamics)扫描之前干燥。用AUTOGENE软件(Biodiscovery，Los Angeles)分析每个点的荧光强度。
在与每个芯片上100的值相当的75％的百分数时的强度读数线性归一化的芯片强度。通过分开那个基因的强度读数，通过在所有的样品上介导基因的表达值将芯片上的每个基因的本身标准化。在簇化之前，至少在一个样品中不具有100或更大的强度读数的基因过滤掉，以便限制影响相似度量的背景。选择一组6,225基因进行成簇分析。利用相关性进行级梯簇化作为相似性的测量，这将显示阳性变化的基因的样品组合在一起，同时不考虑任何负变化(SiliconGenetics，Sunnyville，CA)。然后，将每个树状图中的主要的结考虑为一个样品的亚组。通过将每个肿瘤亚组与正常的组比较，鉴定了不同地表达的基因。选择是根据
的算式给出的噪声测量阈值的信号来进行的，其中μt是平均值肿瘤亚系，μn是正常样品的亚系的平均值，δt+δn代表合并的标准偏差。对于包括在顺式配对比较中的基因，基因的强度读数的变化的组内系数小于0.33。对于上调，肿瘤组的中位值比正常组的中位值大于或等于2，对于下调，小于或等于0.5。如果一个基因符合所有规则，就选择它。在结肠癌中一致地认为不能调节的是在所有的比较中选择的基因。利用假设不等的变化的T测试来计算统计意义的p-值。利用软件包(Partek， St Louis，MO)将设置簇化的基因进行基本成分的分析(PCA)。然后，将数据设计在减小的3维空间中。用设计的表达水平代表正常的和肿瘤结肠样品。
设计具有大的上调差别的基因的表来区别肿瘤和正常的样品。用 作为噪声截止的信号预选择123个基因。比例等于或大于1.5是上调的最小规则。如果存在
这些基因也包括在其中。建立四个基因的组合体，每个在肿瘤和正常细胞之间至少具有3倍的表达差异。
在结肠癌中基因不同地表达。当与正常的结肠粘膜比较时，39个基因在所有的肿瘤样品中不同地表达。他们中的37个在所有的肿瘤中明显下调，除了一个超偏值。他们中的两个是上调的。对微阵列上放置的cDNA克隆进行测序证明基因的某同性。结果表示在表1。表1 受调节的基因
| 入藏号 | 基因描述 | 平均信号强度(正常) | 平均信号强度(肿瘤) | P-值 | |
| AF071569 | CaM激酶II基因亚型δ | 93 | 39 | 4.64E-09 | Seq.ID No.1 |
| AB014530 | 人mRNA for KIAA0630蛋白质 | 108 | 50 | 4.83E-07 | Seq.ID No.2 |
| AK000319 | 人cDNA KIAA0630 | 236 | 69 | 7.84E-06 | Seq.ID No.3 |
| U81504 | Beta-3A-adaptin subunit of the AP-3complexmRNA， | 241 | 75 | 3.52E-05 | Seq.ID No.4 |
| AB011166 | 人cDNAKIAA0594 | 116 | 55 | 3.53E-05 | Seq.ID No.5 |
| AB040914 | 人cDAKIAA1481 | 187 | 59 | 8.85E-05 | Seq.ID No.6 |
| AK025205 | 人cDNAFLJ21552 | 322 | 97 | 0.00013 | Seq.ID No.7 |
| AJ278219 | 脂肪酸羟化酶 | 143 | 53 | 0.00011 | Seq.ID No.8 |
| AB046854 | 人cDNAKIAA1634 | 142 | 59 | 0.00020 | Seq.ID No.9 |
| R00585 | 未知 | 149 | 57 | 1.28E-09 | Seq.ID No.10 |
| S45844 | Spi-B转录因子 | 140 | 43 | 0.00043 | Seq.ID No.11 |
| X98311 | 癌胚抗原家族成员2(CGM2) | 6137 | 223 | 0.00044 | Seq.ID No.12 |
| BAA78050 | NADPH氧化还原酶同系物 | 153 | 84 | 0.00048 | Seq.ID No.40 |
| N72128 | 未知 | 164 | 77 | 0.00068 | Seq.ID No.13 |
| AB040955 | 人cDNAKIAA1552 | 334 | 120 | 0.00067 | Seq.ID No.14 |
| AF125101 | HSPC040蛋白质 | 363 | 115 | 0.0011 | Seq.ID No.15 |
| AB023229 | 人cDNAKIAA1012 | 263 | 88 | 0.00099 | Seq.ID No.16 |
| N95761 | a-L岩藻糖苷酶基因 | 429 | 104 | 0.00047 | Seq.ID No.17 |
| AK025033 | 人cDNAFLJ21380 | 180 | 85 | 0.0010 | Seq.ID No.18 |
| L10844 | 人细胞生长调节蛋白 | 206 | 101 | 0.0013 | Seq.ID No.19 |
| H96534 | gp25L2蛋白质人mRNA | 147 | 58 | 0.0015 | Seq.ID No.20 |
| AK001521 | 人cDNAFLJ10659 | 157 | 60 | 0.0019 | Seq.ID No.21 |
| AF151039 | HSPC205蛋白质 | 117 | 60 | 0.0017 | Seq.ID No.22 |
| AF052059 | SEL 1L蛋白质 | 168 | 53 | 0.0016 | Seq.ID No.23 |
| N24597 | 未知 | 166 | 62 | 0.0016 | Seq.ID No.24 |
| AK001950 | 内部丝粒蛋白质 | 148 | 64 | 0.0029 | Seq.ID No.25 |
| BAA02649 | Macrophage scavenger receptor typel | 118 | 44 | 0.0031 | Seq.ID No.41 |
| N75004 | 未知 | 98 | 48 | 0.0031 | Seq.ID No.26 |
| W16916 | 人cDNAKIAA0260 | 162 | 61 | 0.0037 | Seq.ID No.27 |
| X52001 | 人内皮素3mRNA | 89 | 33 | 0.0042 | Seq.ID No.28 |
| T50788 | 未知 | 364 | 102 | 0.0059 | Seq.ID No.38 |
| AJ005866 | 推测的Sqv-7类似蛋白质 | 381 | 163 | 0.0049 | Seq.ID No.29 |
| AF113535 | MAID蛋白质 | 218 | 100 | 0.0053 | Seq.ID No.39 |
| AB037789 | 人cDNAKIAA1368 | 164 | 62 | 0.0068 | Seq.ID No.30 |
| M33987 | 碳酸酐酶 | 652 | 46 | 0.0074 | Seq.ID No.31 |
| M77830 | 桥粒斑蛋白1(DPI) | 184 | 81 | 0.0092 | Seq.ID No.32 |
| H81220 | EST区转录因子ELF1 | 113 | 55 | 0.017 | Seq.ID No.33 |
| AF000592 | 人染色体21q11-q21基因组克隆 | 33 | 69 | 1.16E-05 | Seq.ID No.35 |
| AK021701 | 人cDNAFLJ11639 | 31 | 63 | 0.00070 | Seq.ID No.36 |
实施例4：结肠肿瘤的最佳组合体
利用平均变化最佳算式产生基于多个基因的特征，其中包括的基因可以联合用于区别正常和肿瘤样品。利用这些样品和实施例1-3所述的微阵列进行强度的测量。首先，根据在肿瘤和正常细胞之间预限定的5倍差异来预选择待分析的数据。然后，如下利用根据这一规则预选择的基因产生的表达数据。利用未转移的样品作为基准计算每个基因的强度测量的平均和标准偏差。然后，计算每个基准基因(X的指定值为3)的差别值X*(标准偏差+平均值)。将这一值用于保证得到的组合体是严格的。然后，计算每个转移的样品中差别值与基准值的比例。然后，将这一比例转化成普通的对数。然后，将这一数据输入Wagner软件，产生了有效的前沿，从中选择了一个4基因的组合体。包括未知序列的系列，前胶原类型I，大亚单位核糖体蛋白质L21，和纤连蛋白。这些基因鉴定为Seq.ID No 42，Seq.ID No.43，Seq.ID No.44，和Seq.ID.No.49(进一步如下鉴定)。或者，用于制作组合体的基因的联合可以用于产生诊断信息，用于进行关于结肠癌的临床决定。这在从组合体中选择基因的联合是与其它标记结合的情况(遗传的或不是)中是特别有益的。
为最佳组合体选择的基因：
＞gi|1264443|gb|N92134.1|N92134 za 23f09.rl soares胎盘肝脾INFLS人cDNA克隆IMAGE：293417 5′相似于gb|M87908|HUMALNE 32人癌症细胞起源的Alu RNA转录物，(rRNA)；gb：X57025 mal类胰岛素生长因子IA前体(人)
＞gi|2221047|gb|AA490172.1|AA490172 ab06b08.s1 Stratagene胎盘视黄素937202人cDNA克隆IMAGE：839991 3′相似于gb：J03464 PROCOLLAGENalpha 2(I)CHAINPRECURSOR(人)
＞gi|2188918|gb|AA464034.1|AA464034.1|AA464034 zx86b09.r1 Soares卵巢肿瘤NbHOT人cDNA克隆IMAGE：810617 5′相似于SW：RL21人P46778 60S核糖体蛋白质L21。
＞gi|1834491|gb|R62612.1|R62612 yi12d01.s1 Soares胎盘Nb2HP人cDNA克隆IMAGE：139009 3′相似于gb：X02761_cdsl纤维素前体(人)；
利用不同系列的规则但相同的方法，通过软件选择另外四个基因的组合体。这些是Seq.IDno.46，Seq.ID no.47，Seq.ID no.48和Seq.ID no.45。两个基因与开始的四个基因组合体交迭。两个最佳的组合体也可以合并形成一个6基因的组合体。
最佳基因组合体：
＞gi|2114953|gb1AA431245.1|AA431245 zw 78d06.r1 Soares_testis_NHT人cDNA克隆IMAGE：782315 5′相似于WP：F36H1.2 CE05814 ANKYRIN LIKE
＞gi|12156172|gb|AA443497.1|AA443497 zw34d03.r1 Soares卵巢肿瘤NbHOT人cDNA克隆IMAGE：771173
＞gi|2221047|gb|AA490172.1|AA490172 ab 06b08.s1 Stratagene胎盘视黄素937202人cDNA克隆IMAGE：839991 3′相似于gb：J03464 PROCOLLAGENALPHA 2(I)CHAIN PRECURSOR(人)
＞gi|1264443|gb|N92134.1|N92134 za 23f09.r1 Soares胎盘肝脾INFLS人cDNA克隆IMAGE：293417 5′相似于gb|M87908|HUMALNE 32人癌症细胞起源的Alu RNA转录物，(rRNA)；gb：X57025_rnal胰岛素类似生长因子IA前体(人)；
序列表
<110>Wang，Yixin
<120>肠癌诊断
<130>CDS 266 US NP
<150>60/368,687
<151>2002-03-29
<160>49
<170>PatentIn version 3.1
<210>1
<211>1500
<212>DNA
<213>人
<400>1
atggcttcga ccaccacctg caccaggttc acggacgagt atcagctttt cgaggagctt 60
ggaaaggggg cattctcagt ggtgagaaga tgtatgaaaa ttcctactgg acaaggatat 120
gctgccaaaa ttatcaacac caaaaagctt tctgctaggg atcatcagaa actagaaaga 180
gaagctagaa tctgccgtct tttgaagcac cctaatattg tgcgacttca tgatagcata 240
tcagaagagg gctttcacta cttggtgttt gatttagtta ctggaggtga actgtttgaa 300
gacatagtgg caagagaata ctacagtgaa gctgatgcca gtcattgtat acagcagatt 360
ctagaaagtg ttaatcattg tcacctaaat ggcatagttc acagggacct gaagcctgag 420
aatttgcttt tagctagcaa atccaaggga gcagctgtga aattggcaga ctttggctta 480
gccatagaag ttcaagggga ccagcaggcg tggtttggtt ttgctggcac acctggatat 540
ctttctccag aagttttacg taaagatcct tatggaaagc cagtggatat gtgggcatgt 600
ggtgtcattc tctatattct acttgtgggg tatccaccct tctgggatga agaccaacac 660
agactctatc agcagatcaa ggctggagct tatgattttc catcaccaga atgggacacg 720
gtgactcctg aagccaaaga cctcatcaat aaaatgctta ctatcaaccc tgccaaacgc 780
atcacagcct cagaggcact gaagcaccca tggatctgtc aacgttctac tgttgcttcc 840
atgatgcaca gacaggagac tgtagactgc ttgaagaaat ttaatgctag aagaaaacta 900
aagggtgcca tcttgacaac tatgctggct acaaggaatt tctcagcagc caagagtttg 960
ttgaagaaac cagatggagt aaaggagtca actgagagtt caaatacaac aattgaggat 1020
gaagatgtga aagcacgaaa gcaagagatt atcaaagtca ctgaacaact gatcgaagct 1080
atcaacaatg gggactttga agcctacaca aaaatctgtg acccaggcct tactgctttt 1140
gaacctgaag ctttgggtaa tttagtggaa gggatggatt ttcaccgatt ctactttgaa 1200
aatgctttgt ccaaaagcaa taaaccaatc cacactatta ttctaaaccc tcatgtacat 1260
ctggtagggg atgatgccgc ctgcatagca tatattaggc tcacacagta catggatggc 1320
agtggaatgc caaagacaat gcagtcagaa gagactcgtg tgtggcaccg ccgggatgga 1380
aagtggcaga atgttcattt tcatcgctcg gggtcaccaa cagtacccat caagccaccc 1440
tgtattccaa atgggaaaga aaacttctca ggaggcacct ctttgtggca aaacatctga 1500
<210>2
<211>5761
<212>DNA
<213>人
<400>2
cacaccgcag tatgcggtgc cctttactct gagctgcgca gccggccggc cggcgctggt 60
tgaacagact gccgctgtac tggcgtggcc tggagggact cagcaaattc tcctgccttc 120
aacttggcaa cagttgcctg gggtagctct acacaactct gtccagccca cagcaatgat 180
tccagaggcc atggggagtg gacagcagct agctgactgg aggaatgccc actctcatgg 240
caaccagtac agcactatca tgcagcagcc atccttgctg actaaccatg tgacattggc 300
cactgctcag cctctgaatg ttggtgttgc ccatgttgtc agacaacaac aatccagttc 360
cctcccttcg aagaagaata agcagtcagc tccagtctct tccaagtcct ctctagatgt 420
tctgccttcc caagtctatt ctctggttgg gagcagtccc ctccgcacca catcttctta 480
taattccttg gtccctgtcc aagatcagca tcagcccatc atcattccag atactcccag 540
ccctcctgtg agtgtcatca ctatccgaag tgacactgat gaggaagagg acaacaaata 600
caagcccagt agctctggac tgaagccaag gtctaatgtc atcagttatg tcactgtcaa 660
tgattctcca gactctgact cttctttgag cagcccttat tccactgata ccctgagtgc 720
tctccgaggc aatagtggat ccgttttgga ggggcctggc agagttgtgg cagatggcac 780
tggcacccgc actatcattg tgcctccact gaaaactcag cttggtgact gcactgtagc 840
aacccaggcc tcaggtctcc tgagcaataa gactaagcca gtcgcttcag tgagtgggca 900
gtcatctgga tgctgtatca cccccacagg gtatcgagct caacgcgggg ggaccagtgc 960
agcacaacca ctcaatctta gccagaacca gcagtcatcg gcggctccaa cctcacagga 1020
gagaagcagc aacccagccc cccgcaggca gcaggcgttt gtggcccctc tctcccaagc 1080
cccctacacc ttccagcatg gcagcccgct acactcgaca gggcacccac accttgcccc 1140
ggcccctgct cacctgccaa gccaggctca tctgtatacg tatgctgccc cgacttctgc 1200
tgctgcactg ggctcaacca gctccattgc tcatcttttc tccccacagg gttcctcaag 1260
gcatgctgca gcctatacca ctcaccctag cactttggtg caccaggtcc ctgtcagtgt 1320
tgggcccagc ctcctcactt ctgccagcgt ggcccctgct cagtaccaac accagtttgc 1380
cacccaatcc tacattgggt cttcccgagg ctcaacaatt tacactggat acccgctgag 1440
tcctaccaag atcagccagt attcctactt atagttggtg agcatgaggg aggaggaatc 1500
atggctacct tctcctggcc ctgcgttctt aatattgggc tatggagaga tcctccttta 1560
ccctcttgaa atttcttagc cagcaacttg ttctgcaggg gcccactgaa gcagaaggtt 1620
tttctctggg ggaacctgtc tcagtgttga ctgcattgtt gtagtcttcc caaagtttgc 1680
cctattttta aattcattat ttttgtgaca gtaattttgg tacttggaag agttcagatg 1740
cccatcttct gcagttacca aggaagagag attgttctga agttaccctc tgaaaaatat 1800
tttgtctctc tgacttgatt tctataaatg cttttaaaaa caagtgaagc ccctctttat 1860
ttcattttgt gttattgtga ttgctggtca ggaaaaatgc tgatagaagg agttgaaatc 1920
tgatgacaaa aaaagaaaaa ttactttttg tttgtttata aactcagact tgcctatttt 1980
attttaaaag cggcttacac aatctccctt ttgtttattg gacatttaaa cttacagagt 2040
ttcagttttg ttttaatgtc atattatact taatgggcaa ttgttatttt tgcaaaactg 2100
gttacgtatt actctgtgtt actattgaga ttctctcaat tgctcctgtg tttgttataa 2160
agtagtgttt aaaaggcagc tcaccatttg ctggtaactt aatgtgagag aatccatatc 2220
tgcgtgaaaa caccaagtat tctttttaaa tgaagcacca tgaattcttt tttaaattat 2280
tttttaaaag tctttctctc tctgattcag cttaaatttt tttatcgaaa aagccattaa 2340
ggtggttatt attacatggt ggtggtggtt ttattatatg caaaatctct gtctattatg 2400
agatactggc attgatgagc tttgcctaaa gattagtatg aattttcagt aatacacctc 2460
tgttttgctc atctctccct tctgttttat gtgatttgtt tggggagaaa gctaaaaaaa 2520
cctgaaacca gataagaaca tttcttgtgt atagctttta tacttcaaag tagcttcctt 2580
tgtatgccag cagcaaattg aatgctctct tattaagact tatataataa gtgcatgtag 2640
gaattgcaaa aaatatttta aaaatttatt actgaattta aaaatatttt agaagttttg 2700
taatggtggt gttttaatat tttacataat taaatatgta catattgatt agaaaaatat 2760
aacaagcaat ttttcctgct aacccaaaat gttatttgta atcaaatgtg tagtgattac 2820
acttgaattg tgtacttagt gtgtatgtga tcctccagtg ttatcccgga gatggattga 2880
tgtctccatt gtatttaaac caaaatgaac tgatacttgt tggaatgtat gtgaactaat 2940
tgcaattata ttagagcata ttactgtagt gctgaatgag caggggcatt gcctgcaagg 3000
agaggagacc cttggaattg ttttgcacag gtgtgtctgg tgaggagttt ttcagtgtgt 3060
gtctcttcct tccctttctt cctccttccc ttattgtagt gccttatatg ataatgtagt 3120
ggttaataga gtttacagtg agcttgcctt aggatggacc agcaagcccc cgtggaccct 3180
aagttgttca ccgggattta tcagaacagg attagtagct gtattgtgta atgcattgtt 3240
ctcagtttcc ctgccaacat tgaaaaataa aaacagcagc ttttctcctt taccaccacc 3300
tctacccctt tccattttgg attctcggct gagttctcac agaagcattt tccccatgtg 3360
gctctctcac tgtgcgttgc taccttgctt ctgtgagaat tcaggaagca ggtgagagga 3420
gtcaagccaa tattaaatat gcattctttt aaagtatgtg caatcacttt tagaatgaat 3480
ttttttttcc ttttcccatg tggcagtcct tcctgcacat agttgacatt cctagtaaaa 3540
tatttgcttg ttgaaaaaaa catgttaaca gatgtgttta taccaaagag cctgttgtat 3600
tgcttaccat gtccccatac tatgaggaga agttttgtgg tgccgctggt gacaaggaac 3660
tcacagaaag gtttcttagc tggtgaagaa tatagagaag gaaccaaagc ctgttgagtc 3720
attgaggctt ttgaggtttc ttttttaaca gcttgtatag tcttggggcc cttcaagctg 3780
tgaaattgtc cttgtactct cagctcctgc atggatctgg gtcaagtaga aggtactggg 3840
gatggggaca ttcctgccca taaaggattt ggggaaagaa gattaatcct aaaatacagg 3900
tgtgttccat ccgaattgaa aatgatatat ttgagatata attttaggac tggttctgtg 3960
tagatagaga tggtgtcaag gaggtgcagg atggagatgg gagatttcat ggagcctggt 4020
cagccagctc tgtaccaggt tgaacaccga ggagctgtca aagtatttgg agtttcttca 4080
ttgtaaggag taagggcttc caagatgggg caggtagtcc gtacagccta ccaggaacat 4140
gttgtgtttt ctttattttt taaaatcatt atattgagtt gtgttttcag cactatattg 4200
gtcaagatag ccaagcagtt tgtataattt ctgtcactag tgtcatacag ttttctggtc 4260
aacatgtgtg atctttgtgt ctcctttttg ccaagcacat tctgattttc ttgttggaac 4320
acaggtctag tttctaaagg acaaattttt tgttccttgt cttttttctg taagggacaa 4380
gatttgttgt ttttgtaaga aatgagatgc aggaaagaaa accaaatccc attcctgcac 4440
cccagtccaa taagcagata ccacttaaga taggagtcta aactccacag aaaaggataa 4500
taccaagagc ttgtattgtt accttagtca cttgcctagc agtgtgtggc tttaaaaact 4560
agagattttt cagtcttagt ctgcaaactg gcatttccga ttttccagca taaaaatcca 4620
cctgtgtctg ctgaatgtgt atgtatgtgc tcactgtggc tttagattct gtccctgggg 4680
ttagccctgt tggccctgac aggaagggag gaagcctggt gaatttagtg agcagctggc 4740
ctgggtcaca gtgacctgac ctcaaaccag cttaaggctt taagtcctct ctcagaactt 4800
ggcatttcca acttcttcct ttccgggtga gagaagaagc ggagaagggt tcagtgtagc 4860
cactctgggc tcatagggac acttggtcac tccagagttt ttaatagctc ccaggaggtg 4920
atattatttt cagtgctcag ctgaaatacc aaccccagga ataagaactc catttcaaac 4980
agttctggcc attctgagcc tgcttttgtg attgctcatc cattgtcctc cactagaggg 5040
gctaagcttg actgccctta gccaggcaag cacagtaatg tgtgttttgt tcagcattat 5100
tatgcaaaaa ttcactagtt gagatggttt gttttaggat aggaaatgaa attgcctctc 5160
agtgacagga gtggcccgag cctgcttcct attttgattt tttttttttt taactgatag 5220
atggtgcagc atgtctacat ggttgtttgt tgctaaactt tatataatgt gtggtttcaa 5280
ttcagcttga aaaataatct cactacatgt agcagtacat tatatgtaca ttatatgtaa 5340
tgttagtatt tctgctttga atccttgata ttgcaatgga attcctactt tattaaatgt 5400
atttgatatg ctagttattg tgtgcgattt aaactttttt tgctttctcc ctttttttgg 5460
ttgtgcgctt tcttttacaa caagcctcta gaaacagata gtttctgaga attactgagc 5520
tatgtttgta atgcagatgt acttagggag tatgtaaaat aatcatttta acaaaagaaa 5580
tagatattta aaatttaata ctaactatgg gaaaagggtc cattgtgtaa aacatagttt 5640
atctttggat tcaatgtttg tctttggttt tacaaagtag cttgtatttt cagtattttc 5700
tacataatat ggtaaaatgt agagcaattg caatgcatca ataaaatggg taaattttct 5760
g 5761
<210>3
<211>2129
<212>DNA
<213>人
<400>3
ctgtattgag acaaaggaag ggatctgtca gaaagcaaca cttgttatct tgggcttggc 60
agcaaggaag aggacaggta gtggagatcc tgcaatctga aaagcagact gaaaggtgac 120
aaagaagctg aagatgggtg gtggagagag gtataacatt ccagcccctc aatctagaaa 180
tgttagtaag aaccaacaac agcttaacag acagaagacc aaggaacaga attcccagat 240
gaagattgtt cataagaaaa aagaaagagg acatggttat aactcatcag cagctgcctg 300
gcaggccatg caaaatgggg ggaagaacaa aaattttcca aataatcaaa gttggaattc 360
tagcttatca ggtcccaggt tactttttaa atctcaagct aatcagaact atgctggtgc 420
caaatttagt gagccgccat caccaagtgt tcttcccaaa ccaccaagcc actgggtccc 480
tgtttccttt aatccttcag ataaggaaat aatgacattt caacttaaaa ccttacttaa 540
agtacaggta taaaataaga caaatgttta aatttagtta tgttcacggg tagttgtcaa 600
ttggtctgaa acaaatttgc tagggaatct atttgtgtag aactaattaa tgtaaaaaaa 660
atagaccatc tcgtgttgtg tgcactgtga tataatggta gtatcagtgc aacttaaact 720
aatgattgta cttgatatta agtgttctca actgagtaac ttttaagtgg aaaccaagtt 780
tagatttggg gagtggtaaa ggaatcagct ttttctattg ttaggggaag acagtaattt 840
atcattcatg gaccagtaga ttgttgaaag ttggtgaatc ggattataag cttctagcta 900
acacaaggat tcagaattag gtaaacatct gaaggtttag tatattagaa acacccaaac 960
cagtaatatg ctaacctgat gcactgctga aagaaaatgt gaatttttcg taataattgc 1020
attttagtga attgtacagt gggtggaaag ggcatttgga gctcattaga atgagacata 1080
gtacacccca atggccctgt ttattaaatg tagtggatta agtgtctgtc aacaaataca 1140
ccaaaaccat tttttataga aacagtattt aatggtcact caatagcttt caaaatacat 1200
ttttgtatta cagcactgca caagctattc taatagtgct ctggcctcat cattcctgca 1260
aagcttgctt tggggagttg gataatgtga aaattttaag tacctagggg agaaagagcc 1320
atgtaaatat ctgtaataaa cttgtagcat atgtaaagtt ttcttggcct ttatcttaca 1380
aaaatggagt attttagtat gaatttgctg aatgtaagac cgtggactgt tttttataat 1440
atggcctaat tttaaaggtc caaaataact tgtttttaaa gtttgccctt gtgctaaagt 1500
gccagtgtat gtatgttata cttgatttgg ttgtaaacta tatttcaaag taaaccctag 1560
tgtaataagt tttataacta aaaaggttta agctgctaaa actattttta agagatgtga 1620
aatgcagtat gggactatct ttttttcctc ctctaagccc aaagattaac tagagtccct 1680
ccaaccttat agattgttgg ctttcacaat cttataacct aggatacagg tagtttcgag 1740
tatggtgcca gtgatgtttt gtttttgttt ggtcaagggg taggtgcaac ccaatggacc 1800
acttatgcaa aagatgtaaa ctcttgcata atacattgat aacatgtttt gccaacttta 1860
aatgcttaaa cataagcgaa accagtagca agtatgtggg tcagcttaaa aattttgatt 1920
gttaatgccc tattttctaa tttggcacct cttgatgcct aagcaggtaa gcagatgcct 1980
aagctgtatt tctccaaata aatcaagatg aagtactgcc caagttaaat attgatagcc 2040
taaagacaag tttatgtagt acttaatgta catgatatga agcataaaat taaataaaat 2100
ttttccccat tgaaaaaaaa aaaaaaaaa 2129
<210>4
<211>3950
<212>DNA
<213>人
<400>4
cgagaactag ttttgttccg tgccctctgg actggaacct tttggagaga acccccggca 60
ggaccaaccc cgcacccgcc agcaccgcgg caatgtccag caatagtttt ccttacaatg 120
agcagtccgg aggaggggag gcgacggagc tgggtcagga ggcgacctca accatttccc 180
cctcgggggc cttcggcctc tttagcagcg atttgaagaa gaatgaagat ctaaagcaaa 240
tgttagagag caacaaagat tctgctaaac tggatgctat gaagcggatt gttgggatga 300
ttgcaaaagg gaaaaatgca tctgaactgt ttcctgctgt tgtgaagaat gtggccagta 360
aaaatattga gatcaagaag ttggtatatg tttacctggt tcgatatgct gaagaacagc 420
aggatcttgc actcctgtcc ataagcactt ttcagcgagc tctgaaggac ccaaaccaac 480
taattcgtgc aagcgctttg agagttctgt caagtattag agtgccaatt attgtaccta 540
tcatgatgct tgctattaag gaagcttctg ctgacttatc accatatgtt aggaagaatg 600
cagcccatgc aatacaaaaa ttatacagcc ttgatccaga gcagaaggaa atgttaattg 660
aagtaattga aaaacttctg aaagataaaa gcacattggt agctggcagt gttgtgatgg 720
cttttgaaga agtatgcccg gacagaatag atctgattca taaaaattac cgcaagctat 780
gtaacttact agtggatgtt gaagagtggg ggcaggttgt cataatccac atgctaactc 840
gatatgctcg gacacagttt gtcagccctt ggaaagaggg tgatgaatta gaagacaatg 900
gaaagaattt ctacgaatct gatgatgatc agaaggaaaa gactgacaaa aagaagaagc 960
cgtatactat ggatccagat catagactct taattagaaa tacaaagcct ttgcttcaga 1020
gcaggaatgc tgcggtggtt atggcagttg ctcagctgta ttggcacata tcaccaaaat 1080
ctgaagctgg cataatttct aaatcactag tgcgtttact tcgtagcaat agggaggtgc 1140
agtatattgt cctacaaaat atagcaacta tgtcaattca aagaaagggg atgtttgaac 1200
cttatctgaa gagtttctat gttaggtcaa ctgatccaac tatgatcaag acactgaagc 1260
ttgaaatttt gacaaacttg gcaaatgaag ccaacatatc aactcttctt cgagaatttc 1320
agacctatgt gaaaagccag gataaacaat ttgcagcagc cactattcag actataggca 1380
gatgtgcaac caacatcttg gaagtcactg acacgtgcct caatggcttg gtctgtctgc 1440
tgtccaacag ggatgaaata gttgttgctg aaagtgtggt tgttataaag aaattactgc 1500
aaatgcaacc tgcacaacat ggtgaaatta ttaaacatat ggccaaactc ctggacagta 1560
tcactgttcc tgttgctaga gcaagtattc tttggctaat tggagaaaac tgtgaacgag 1620
ttcctaaaat tgcccctgat gttttgagga agatggctaa aagcttcact agtgaagatg 1680
atctggtaaa actgcagata ttaaatctgg gagcaaaatt gtatttaacc aactccaaac 1740
agacaaaatt gcttacccag tacatattaa atctcggcaa gtatgatcaa aactacgaca 1800
tcagagaccg tacaagattt attaggcagc ttattgttcc gaatgaaaag agtggagctt 1860
taagtaaata tgccaaaaaa atattcctag cacaaaagcc tgcaccactg cttgagtctc 1920
cttttaaaga tagagatcat ttccagcttg gcaccttatc tcatactctc aacattaaag 1980
ctactgggta cctggaatta tctaattggc cagaggtggc gcccgaccca tcagttcgaa 2040
atgtagaagt aatagagttg gcaaaagaat ggaccccagc aggaaaagca aagcaagaga 2100
attctgctaa gaagttttat tctgaatctg aggaagagga ggactcttct gatagtagca 2160
gtgacagtga gagtgaatct ggaagtgaaa gtggagaaca aggcgaaagt ggggaggaag 2220
gagacagcaa tgaggacagc agtgaggact cctccagtga gcaggacagt gagagtggac 2280
gggagtcagg cctagaaaac aaaagaacag ccaagaggaa ctcaaaagcc aaaggaaaaa 2340
gtgattctga agatggggag aaggaaaatg aaaaatctaa aacttcagat tcttcaaatg 2400
acgaatctag ttcaatagaa gacagttctt ccgattctga atcagagtca gaacctgaaa 2460
gtgaatctga atccagaaga gtcactaagg agaaagaaaa gaaaacaaag caagatagaa 2520
ctcctcttac caaagatgtt tcacttctag atctggatga ttttaaccca gtatccactc 2580
cagttgcact tcccacacca gctctttctc caagtttgat ggctgatctt gaaggtttac 2640
acttgtcaac ttcctcttca gtcatcagtg tcagtactcc tgcatttgta ccaacgaaaa 2700
ctcacgtgct gcttcatcga atgagtggaa aaggactagc tgcccattat ttctttccaa 2760
gacagccttg catttttggt gataagatgg tctctataca aataacactg aataacacta 2820
ctgatcgaaa gatagaaaat atccacatag gggaaaaaaa acttcctata ggcatgaaaa 2880
tgcatgtttt taatccaata gactctcttg agcctgaggg atccattaca gtttcaatgg 2940
gtattgactt ttgtgattct actcagactg ccagtttcca gttgtgtacc aaggatgatt 3000
gcttcaatgt taatattcag ccacctgttg gagaactgct tttacctgtg gccatgtcag 3060
agaaagattt taagaaagag caaggagtgc taacaggaat gaatgaaact tctgctgtaa 3120
tcattgctgc accacagaat ttcactccct ctgtgatctt tcagaaggtt gtaaatgtag 3180
ccaatgtagg tgcagtccct tctggccagg ataatataca caggtttgca gctaaaactg 3240
tgcacagtgg gtcattgatg ctagtcacag tggaactgaa ggaaggctct acagcccagc 3300
ttatcataaa cactgagaaa actgtgattg gctctgttct gctgcgggaa ctgaagcctg 3360
tcctgtctca ggggtaacct gcttacatct ggactttaga atctggcaca caacaaaagt 3420
gcctggcatc cactactgct gcctttcatt tataataata gcccttccat ctggcagtgg 3480
gggtagaata cactcttgac attcttgtct cctgctttag aatgctagtg tgtatctatc 3540
atgtatgcaa tactttcccc ctttttgctt tgctaaccga agagcatata ttttactgtc 3600
agttgtctca actcttgaat ccatgtggcg ttttctctgt cctgctgctt cttttggcct 3660
cctcgttttc cttctctttt tcgacaatgg tagacatgaa tgagatattt aaagttcatt 3720
ggaaatcttc ttccctacag cagtaagcaa aaattagcaa agagatagtc taaatggcct 3780
ctcagcttgg tatgtgaaaa tgagatcaca tactttttaa atccaaatac aaaagcatag 3840
tctctgcaag attttgttct ttgaatttct tgatattgta attgattatt gataactgtc 3900
atcatgaaat tatctctcaa taataagata aataaactag catatgaatc 3950
<210>5
<211>5191
<212>DNA
<213>人
<400>5
gagaaagaaa aacagctcga gacctcatgc aaagagaaaa ctgagtatct acagaaaatg 60
gttcagagga atgaaagata taaacaagat gtggagaggt tctatgaacg gaagcgacat 120
ttagatttaa ttgagatgct tgaagcaaaa aggccatggg tggaatatga aaatgttcgt 180
caggaatatg aagaagtaaa actagttcgt gaccgagtga aggaagaggt cagaaaactt 240
aaagaagggc agattcctat aacatgtcga attgaagaaa tggaaaacga gcgtcacaat 300
ttggaggctc gaatcaaaga aaaggcaaca gatattaagg aggcatctca aaaatgcaaa 360
cagaagcaag atgttataga aaggaaagat aaacatattg aggaacttca gcaggcttta 420
atagtaaagc aaaatgaaga gcttgaccga cagaggagaa taggtaatac ccgcaaaatg 480
atagaggatt tgcaaaatga actaaagacc acggaaaact gcgagaatct tcagccccag 540
attgatgcca ttacaaatga tctgagacgg attcaggatg aaaaggcatt atgtgaaggc 600
gaaataattg ataagcgaag agagagggaa actctagaga aggagaaaaa gagtgtggac 660
gatcatattg tacgttttga caatcttatg aatcagaagg aagataagct aagacagaga 720
ttccgtgaca cgtatgatgc tgttttatgg ctaagaaata acagagacaa atttaaacaa 780
agagtctgtg agcccataat gctcacgatc aatatgaaag ataataaaaa tgccaaatat 840
attgaaaatc atattccatc aaatgactta agagcctttg tatttgaaag tcaagaagat 900
atggaggttt tcctcaaaga ggttcgtgac aataaaaaat taagagtaaa tgctgttatt 960
gctcccaaga gttcatatgc agacaaagca ccttcaagat ctttgaatga acttaaacaa 1020
tacggatttt tctcttattt gagagaatta tttgatgcac ctgatcctgt aatgagttac 1080
ctttgctgtc agtatcatat tcatgaagtt cctgtaggaa ctgaaaagac cagagaaaga 1140
attgaacggg taatacaaga aacccgatta aaacagattt atacagcaga agaaaagtat 1200
gtggtgaaaa cttcttttta ttcaaacaaa gttatttcta gtaacacatc tctaaaagta 1260
gcgcagtttc tcactgtcac tgtggaccta gagcagagaa gacacttaga agaacagcta 1320
aaggaaattc atagaaaatt gcaagcagtg gattcagggt tgattgcctt acgtgaaaca 1380
agcaaacatc tggagcacaa agacaatgaa cttagacaaa agaagaagga gcttcttgag 1440
agaaaaacca agaaaagaca actggaacaa aaaatcagtt ccaaactagg aagtttaaag 1500
ctgatggaac aggatacttg caatcttgaa gaggaagagc gaaaagcaag taccaaaatc 1560
aaagaaataa atgttcaaaa agcgaaactt gttaccgaat taacaaacct aataaagatt 1620
tgtacttctt tgcatataca aaaagtagat ttaattctcc aaaatactac agtgatctct 1680
gagaagaaca aattagaatc agattatatg gccgcatctt cacaactccg tcttacagag 1740
caacatttca ttgaattgga tgaaaataga cagagattat tgcagaaatg caaggaactt 1800
atgaaaagag ctaggcaagt atgtaacctg ggtgcagagc agactcttcc tcaagaatac 1860
cagacacaag tacccaccat tccaaatgga cacaactcct cactccccat ggttttccaa 1920
gaccttccaa acacattgga tgaaattgat gctttattaa ctgaagaaag atcaagagct 1980
tcctgcttca cgggactgaa tcctacaatt gttcaggaat atacaaaaag agaagaagaa 2040
atagaacagt taactgagga actaaaggga aagaaagttg aactagatca atacagggaa 2100
aacatttcac aggtaaaaga aaggtggctt aatcctttaa aagagctggt agaaaaaatt 2160
aatgaaaaat tcagcaattt ttttagttcc atgcagtgtg ctggtgaagt tgatctccat 2220
acagaaaatg aggaagatta tgataaatat ggaattcgaa ttagagtcaa atttcgaagt 2280
agtactcaac tgcatgaatt aactcctcat catcaaagtg gaggtgaaag aagtgtttct 2340
accatgttat acttgatggc acttcaggag ctaaatagat gtccattcag agtagttgat 2400
gaaatcaatc agggaatgga cccaatcaat gaacggagag tgtttgaaat ggttgtaaat 2460
actgcctgta aagaaaatac atctcaatac tttttcataa caccaaagct cctgcaaaat 2520
cttccttatt ctgaaaagat gacagttttg tttgtctaca atggccctca tatgctggaa 2580
ccaaacacat ggaatttaaa ggctttccaa aggcggcggc gccgtattac attcactcaa 2640
ccttcttaat aaaagtaaag agagggaact tgggaatttt ttttgttaaa ttctgtttat 2700
aagtatggct caactgaata aaaggagatt cactaaaacg aaaagcagtt atttttggaa 2760
acctgctttt aaatacaaat aggttgataa tggaaactat aatgaccttt ccaaaatagc 2820
agctggtagt aaaagttaag tcttcttcag tcttggttga acttgagttc ttggcactct 2880
gaccatgagt cattcagttc tcatgttaaa atgtacttaa tattacaaat caaaggtaca 2940
gtggaagaag ggttaatcac aagaagttac ttatatggta gccctgagct ttaattgcag 3000
agtaacttta attactttta gagcctaaag atgactctag agcctaagtc ctagtttctc 3060
ccaatgttat atttaatttt aaaaaattga tatgaaaatg tctaatgtat agtaataatt 3120
tatgacagat ctagtcattt cttcctatta aaaaagatta ccttatctcc agtaggaaat 3180
ggaattttat gggcctttaa aagaaagttt tatgaaactt gatgctataa ttttattggt 3240
atttcaaggg gaaaaaagca ctggggttca aaaatggtag cagaactgct ttgaaatgct 3300
gcaaggtggc cactagatga tgcaaaatac aaccaaaaga ttgactgaga ataaaattag 3360
gtgacaaggg tttttaaaga ataacctttt aaagtgtggg ggcaggggtt gctttttttt 3420
attttattta aagtcaatta tattttacat cttacatttc taaaagcatt ttataattat 3480
ttttagtaag atttttctta aaatttcata tactggtttc tacaatttat atttgaaatt 3540
tctcagtgtt atgtaaagag tgatggaaaa gcattgattt ctttaaaacc gtaatgtttt 3600
tagaacttaa gcctataggg cctttcttac aatgttgatg tacccattat cttagaaaat 3660
ctagtttaaa ctgttttctt tcaccgcaaa agaattaaat gggaaaatca tttgtttatc 3720
tctaagttat actaattagt agaaccaaac aaattatctt cttttaaaaa ataaatctta 3780
taggaaaata gacagtccaa agtcatgtct ttgaacagtg gattggatct gtgccagtaa 3840
tgacaaaatt atttttttga cttgcttgcc tgaataaatt gaagaattgc tttcagtttg 3900
ggttttgtat attcttaagt agccattgaa atttatattc ttaactaggt caaaaaataa 3960
tgagccataa gtttatgtcc tctcacttag acattttctc tttaaaaagg tattttcttc 4020
tttataaaca ttttaaaaga gccttccctt cttaaactaa ctccagtgca tgaagtgtga 4080
aaatatttta aaatgacatt tttactaata tgagcaagtc atgtaaacat tgaagaactt 4140
ggtaacatat tagtaaatgg atattaccaa atgttttcat cgttaattac tttgcgttcc 4200
accaaaatat ctttactaaa atgtgcttgg tgtagtttgt ttattgtcta aattagtacc 4260
agtcatctta tttctgcaaa atgagtatca atgtgaaaaa gacacgtgaa gattaagcat 4320
gtttgaaaat aaaatggtca attacatttc aatttacata ggccaacaac tgttccatac 4380
tttgtttgta aacatttaat ttctctactg gacaaaatta atatttggct ttacattgaa 4440
ttttgagctg tgaagaataa attatgtatc attttagcat attaaacagt agtaagtcta 4500
gcacatagtc tcagccactt aaaacaaaag tttttttgtt tgtttgtttg tttgtttttt 4560
tgagatggag tctcactctg ttgcccaggc tggagtgcag tggcgtgatc tcggcttact 4620
gcaacctccg cctcccgggt tcaagcgatt ctcctgcctc agcctcccaa gtaactggga 4680
caacaggcgc gtcccaccac acccagctaa ttttttatac ttttagtaga gatggggttt 4740
cagcatattg gccaggctgg tctcgaactc ctgaccttgt gatccacccg cctcggcctc 4800
ccaaagtgct gggattatag gcgtgagccc ctgcacccgg ccaaaagttg atttttaatt 4860
acataaaaat cgtaaaaact tctagtaaaa acttgatttg gtgaatacag ttatatttta 4920
aaaccttaag gtgacaagca ttttctatgc ctaaatcttc attggtttgc ctggaaagag 4980
tctctgttaa aagattttcc atattcaaag taaaaggaaa gatttcttgc ttcctaattg 5040
tcttttggac acatgcctat tttctttgag gtataaacct ttagatgtga aaaatgtaat 5100
ttcattctgc tattgtgtgt gcttgtgtgt gtgtaattga aaaaactggg aaatcctgct 5160
ttgttggtaa taaatcaata tttttatatt c 5191
<210>6
<211>4755
<212>DNA
<213>人
<400>6
aagagatctt ccaggctctc agagccctgg gagggcgatt tccaggaaga ccacaatgcc 60
aacctctgga ggaggctgga gagagaaggc ctaggccaga gcctgtcagg caactttggc 120
aagaccaagt cagccttctc atctctccag aacattcctg agagtctgag aagacacagc 180
agcctggagc taggccgggg aacccaggag ggttaccccg ggggcaggcc cacctgtgca 240
gtcaacacca aggcagaaga ccctgggagg aaagccgctc ctgacctcgg gagccatctg 300
gaccggcagg tttcctaccc gcggcccgag gggaggaccg gtgcctcggc ttctttcaac 360
agcacagacc caagtcccga agagccgcct gccccctcgc acccgcacac atccagtctg 420
ggccggaggg ggcccggccc aggcagcgcc tcggctcttc agggctttca gtacgggaag 480
ccccactgct cggtgctgga gaaggtctcc aaattcgagc agcgagagca agggagccag 540
agaccgagtg tgggcggctc tggttttggc cataactata ggccccacag gaccgtctca 600
acttccagta cttctgggaa tgacttcgag gagacaaaag cacacattcg tttctctgag 660
tcagctgaac ccctaggcaa cggggagcag cacttcaaaa acggggagct gaagttggaa 720
gaggcttccc ggcagccctg cggtcagcag ctgagcggag gagcgtcgga cagcggccgt 780
ggcccccaga ggccggacgc tcggctcctc cgtagccaga gcaccttcca gctctccagc 840
gagccagaga gggagcccga gtggcgggac aggcccggct cgcccgaatc gcccctgctg 900
gatgccccct tcagccgcgc ctaccggaac agcatcaagg acgcacagtc ccgtgtcttg 960
ggggccacct cctttcgacg tcgagacctg gagctggggg cgcccgtggc gtcgaggtcc 1020
tggcggccac ggccttcctc ggcccacgtg gggctgcgga gccccgaggc gtcggcctcc 1080
gcctccccgc acacgccccg ggagcggcac agcgtgaccc ctgctgaggg cgacctggcc 1140
aggcccgtgc cccctgccgc ccggagaggt gctcgccggc gcctgactcc cgagcagaag 1200
aagcgctcct actcggagcc cgagaagatg aacgaggtgg ggatcgtgga ggaggccgaa 1260
ccggcacccc tgggcccgca gagaaatggg atgcgtttcc cggagagcag cgtggccgac 1320
cggcgccgtc tcttcgagcg cgatggcaag gcctgctcca cgctcagcct gtcggggccc 1380
gagctgaagc agttccagca gagcgccctg gcggactaca tccagcgcaa gaccggcaag 1440
cggcctacct ccgccgccgg ctgcagcctc caggagcccg ggccactgcg tgagcgcgcc 1500
cagagtgcct acctccagcc cggccccgcg gcgctcgaag gctccggcct cgcctcggcc 1560
tccagcttga gctcactgcg ggagcccagc ctgcagcccc gcagggaggc cacgctcctg 1620
ccggccacag ttgcagaaac ccagcaggct ccccgagatc gcagcagctc cttcgccggt 1680
ggccgccgcc tcggggaacg gcgacgcggg gacctgctta gcggagcaaa cggtggaaca 1740
aggggcaccc agagagggga tgagaccccc agggagccat cctcctgggg ggccagggcc 1800
gggaagtcca tgtcggccga ggacctgctg gaacgctcgg acgtccttgc gggccctgtc 1860
catgtgaggt ccaggtcatc tcccgccacc gcagacaagc gccaggatgt gcttttgggg 1920
caagacagtg gctttggtct tgtgaaggat ccatgttatt tggctggtcc tggatctagg 1980
tcactcagtt gttcagaaag aggccaagaa gagatgctgc tgctcttcca ccatctcacc 2040
cctcgttggg gtggttcagg ctgcaaagcc attggtgatt cctccgttcc tagtgaatgt 2100
cctggaaccc tggaccatca gaggcaagcc agtaggacac cctgccccag gccaccactg 2160
gcaggaacgc aagggctggt cacagacacc agggctgcac ccctgacccc aattggcacc 2220
cctctgcctt cagccattcc ctctggctac tgctcacagg acggtcagac agggcgacag 2280
cctctcccgc cctacacccc tgccatgatg cacagaagca atggtcacac cctgacccag 2340
cctcccggtc caagaggctg tgagggcgat ggcccagagc atggggtaga agagggaacg 2400
aggaagaggg tctcgctgcc tcagtggcca cctccttctc gagcaaagtg ggcccacgca 2460
gccagagagg acagccttcc tgaggaatcc tcagcccctg attttgcaaa cctgaagcac 2520
tatcaaaaac agcagagtct tccaagttta tgcagcactt ctgacccaga cacacctctt 2580
ggggccccga gcactccagg gaggatctcc ctccgaatat ctgagtctgt cctgcgggac 2640
tccccgccac ctcatgagga ttatgaagac gaagtgtttg tgagggatcc gcaccccaag 2700
gccacgtcca gccccacatt tgaacctctt cccccacccc cacctcctcc accgagtcag 2760
gaaaccccgg tgtatagcat ggatgacttc cctccacctc ctccccacac tgtatgtgag 2820
gcgcagctgg acagtgagga tcccgagggg ccacgcccca gcttcaacaa actttctaaa 2880
gtgacaattg caagggaaag gcacatgcct ggtgcagccc atgtggtagg tagtcagaca 2940
ctggcttcca gactccaaac ttctatcaag ggttcagagg ctgagtccac accaccctcc 3000
ttcatgagcg ttcacgccca acttgctggg tctcttggtg ggcagccagc acccatccag 3060
actcaaagcc tcagccatga tccagtcagt ggaactcagg gtttagaaaa gaaagtcagt 3120
cctgatcctc agaagagttc agaagacatc agaacagagg ctttggccaa ggaaattgtc 3180
caccaagaca aatctctagc agacattttg gatccagact ccaggctgaa gacaacaatg 3240
gacctgatgg aaggtttgtt tccccgagat gtgaacttgc tgaaggaaaa cagtgtaaag 3300
aggaaggcca tacagagaac tgtcagctct tcaggatgtg aaggcaagag gaatgaagac 3360
aaggaagcag tgagcatgtt ggttaactgc cctgcctact acagtgtgtc tgctcccaag 3420
gctgagctac tgaacaaaat caaagagatg ccagcagaag tgaatgagga agaggaacag 3480
gcagatgtca atgaaaagaa ggctgagctc attggaagtc tcacccacaa gctggagacc 3540
ctccaggagg cgaaggggag cctgctcacg gacatcaagc tcaacaacgc cctgggagaa 3600
gaggtggagg ctctgatcag cgagctctgc aagcccaatg agtttgacaa gtataggatg 3660
ttcatagggg atttggacaa ggtggtcaac ctgctgctct ccctctcggg gcgtctagcc 3720
cgtgttgaga atgtccttag cggccttggt gaagatgcca gtaatgaaga aaggagctct 3780
ctttacgaga aaaggaagat cctggctggt cagcatgagg atgcccggga gctgaaggag 3840
aacctggatc gcagggagcg agtagtgctg ggcatcttgg ccaattacct ttcagaggag 3900
cagctccagg actaccagca cttcgtgaaa atgaagtcca cgctcctcat tgagcaacgg 3960
aagctggatg acaagatcaa gctgggccag gagcaggtca agtgtctgct ggagagcctg 4020
ccctcagatt tcattcccaa ggctggggcc ctggctctgc ccccaaacct cacgagtgag 4080
cccattcctg ctgggggctg tactttcagt ggtattttcc caacattaac ctctccactt 4140
taacctcttc taaaataccc aaccaaaaga tcactgtttc tctcaacact atttaatctg 4200
aaaaatgttt cagtacaaac cactgtttga actatctggg ttattggtgt ttgttcctga 4260
tgaaaggaaa aaaattctct ccaggaggaa gcctttttcc ttcttgccct tcctgattga 4320
tcttctgaga gctcgaatgc tgctggacac gtaccccttt ctattattac tttgtagtag 4380
aaagaaagtt aatgaaactg agaactgatt ggagggtgtt tgatcattta gtttttaaca 4440
ggctgaggca acatggatca gtgtgtgtcc ccctcaggaa tgtatccaca gtggccttcc 4500
ttgctggtgg gcagtgtatc ctgatggcag ggtacaagta ccattaatga agggtctgca 4560
acataaagcc ttaaaaagac acacactaag aaaactgtaa aaccttgaac attgttattt 4620
atatttttta aaatggaaaa gatcactatg tttgttgtgc taaccactta tttgattctg 4680
ttttgtggtg gacatagatg attacgtttg agctttgtat tttgtgaaaa ccttaatgaa 4740
atgaattcca aagat 4755
<210>7
<211>2045
<212>DNA
<213>人
<400>7
gaaacttgac cccggctcat cctgtctctg gctgtggccc ggcaaagcac tgaaaacccc 60
tctggtctca gagacagtag gggcagtgcc actttctaca acctgccaac ccacacactg 120
gagtaattct gaaaaaaatt attcctaaac tctctaagtg tggacggaga atgagcaagc 180
cccagaagta ttttacaacc agagtgggta atgaggaggg ggcttactgg aatcgtcata 240
tctctgaata ttgaaaacaa caactaaaaa agtggacctt ctcagaaaaa aagggcagca 300
aatgaccaag ggcgcccctt ctggccgtgc ttggcttgag taactgtctc tctttcccca 360
cccccatcac agggctttca gtttggcaaa ggaaaagcag ataaaaacag aacattccat 420
atgtttcttt ctccatcggc caaaaacatt ttgacacaat gtttgtgaaa cacctttgga 480
gaggtgcact tctgaatgct gcctctgccg taaatcctgg ggcaagggat cagcctcttc 540
ccaggaacca tcgccttcta taaaccgtga actcaagcag gcattttttt tttcttaccg 600
aaaggctgct attgtgcaag ggcacataat gggtctgttg ctcttattgg cttccaaatg 660
tgcatggcaa agagagagat gtgggcctag agcagatata ttcagcaagg tgacagcttc 720
ccataacaat tctaacactt cttatcttat gtgagaataa aatatttaag ggttgaacct 780
tattttgcca aatgtatctt ttctgctttt gaattgggca gaagatttta gcaactatat 840
tctacaaatg ttacttataa cacacacaca cacatctgaa atatatgccg aaaattgacg 900
tctttgacct cagggagagc acctgtccag gtctgcctaa aggaaatggc tccagtgggt 960
ctaaacaacc acatcctatc catggatagg tctagtcata acactttaga gagaatgtca 1020
gagcaggagg gaggcaagcc gcctcttctc ggccatcaac tgcagatgat gaaagagcgg 1080
gattcaactt tgttttcttt tcctgtggcc ccagtgaaac ctcctgccct ccctgcacgt 1140
ctgtgtcttc atttctaaaa tgggggtgat gctttcatat tgacctcacc ccatactacc 1200
tcacagatgt gttgtgagga ttaataaaat tatgtctatg gtattttcag tttctggaga 1260
aaaatactta tagacagttt aactattaca tagatatata agtgatctca gtttcttgtt 1320
tgctgtgata ctaatgtgtt gttttaactt attccataaa atgacagttg tgtcctagcc 1380
acatcagaca gctatctaag ctctggacta cccctttgtg cagctgaatc actgcagggt 1440
cgaccatgcc tggtgccaca gccatggttt ccatttctag atgaaaggat ggcctaggac 1500
ataggtctca aagactcttg gatcagaatc aggagattag ggaaaacagg atggatacct 1560
gagcactaac agcagtagac gtagacctct gtcctttacc atctgaggtc ttctggattc 1620
tttgtggggt taattttgat ttgatgtcat ctgtttgccc ttcatcttgc ttgcaagtgt 1680
gcatggttca atccctcaca tccaggaaat gaattttgca attgggccag atgctaattt 1740
gcacgttgat tcaccttctt tgcctttaag cctttttttt cttttttttt ttttttggca 1800
aatgaatgta ccatttcaac tttgatttta atagtgctag ttgatattgg taataatgct 1860
aaccaagaga tcaatgccag atttttctct tggggtaagt tagctgaagt catttaaaga 1920
tggaaaggtg ggaaaattct ttgatatttg atgtcattgt atccacattt gttgtaagac 1980
atattgcata ccaattataa ttatatcaat taaagttgat aaaagcttca aaaaaaaaaa 2040
aaaaa 2045
<210>8
<211>2096
<212>DNA
<213>人
<400>8
atggagaacg agcctgtagc ccttgaggaa actcagaaga cagatcctgc tatggaacca 60
cggttcaaag tggtggattg ggacaaggac ctggtggact ggcgaaagcc tctcctgtgg 120
caggtgggcc acttgggaga gaagtacgat gagtgggttc accagccggt gaccaggccc 180
atccgcctct tccactcaga cctcattgag ggcctctcta agactgtctg gtacagtgtc 240
cccatcatct gggtgcccct ggtgctgtat ctcagctggt cctactaccg aacctttgcc 300
cagggcaacg tccgactctt cacgtcattt acaacagagt acacggtggc agtgcccaag 360
tccatgttcc ccgggctctt catgctgggg acattcctct ggagcctcat cgagtacctc 420
atccaccgct tcctgttcca catgaagccc cccagcgaca gctattacct catcatgctg 480
cacttcgtca tgcacggcca gcaccacaag gcacccttcg acggctcccg cctggtcttc 540
ccccctgtgc cagcctccct ggtgatcggc gtcttctact tgtgcatgca gctcatcctg 600
cccgaggcag tagggggcac tgtgtttgcg gggggcctcc tgggctacgt cctctatgac 660
atgacccatt actacctgca ctttggctcg ccgcacaagg gctcctacct gtacagcctg 720
aaggcccacc acgtcaagca ccactttgca catcagaagt caggatttgg tatcagcact 780
aaattgtggg attactgttt ccacaccctc actccagaga aaccccacct gaagacgcag 840
tgacaactcc caccccctcc gtcctgccct cagcccggcc ctggcccctt cccgaccccc 900
acccgccatt cagaccccat taagaaggtt ggcttggcca ggcaggatgg gctgtgtccg 960
gccctgcagc ctagtggaag gtgctgaggg ggccctgagg caggaccgcc ctcctgaccc 1020
ctggtaggag ggtcacatcc acttggtgca ggtggccctt ggtgacccac ttcttcctgg 1080
agcgtccctg cctagagctc agcccacagg actgcttcag gccgtggcca caggtagcag 1140
ccgcaagggg aaatgaagaa aactgagccc tcgtggccac ctgtgtcacc cttgtgcctt 1200
agcctcatgg gctgcctagg agctgcctgc acggcacagc tcgctttcac agtcagaagt 1260
gggtctgtgg gatctgtggt ccctgtcctc cctgctgtcc cttctgggga ggctttggtg 1320
gctctgaggt ggacaaagag ctctcgcaag aagagacagc gtgatgcctc ccacagtcca 1380
ccccagaccc tggggcagcc cctctggccc tgccagctgc ctgcgtcgtt gggcccaggg 1440
tggctggcag gagtcccagc tgcttgcttt aggacctggc agcttttctt gccgtccctc 1500
ccctgcctcc agaatcacag cccttctccc caagggaggc tgaggaggct tctccaccag 1560
tggcagcccc accccgtccc tggccattct tggcctccac cccgctcagg cccctactcg 1620
ggcgctccca gaaggagcca cctctcagtg cctcacctcc ccctgcctcc cagcctccgc 1680
agatgaggtt cctgcccctt cctcctcgta accaaaaccc tcactgctcc caggacggtc 1740
ttatttataa accagataca tgttcttagt ctggtcccag accaaggagc tggtcagacg 1800
gccctttcta atcctacatg ttgagcttat gtaaaaaatg ttgtttcctc ctgtttttgg 1860
ttcctttctt acccacaaac cattactact tgaaacttaa aaaactcgcc aagtgtaaag 1920
gctaaagaga agcagtttga cggaccttgt gatttgtact gtttgctgcg gagctattta 1980
aagattttgg aataaatata caaaactacg gttgtgaaat aaaaacttaa attgtatatt 2040
ttgaaaaata aaacactgaa aagaaaccaa caaaaaaaaa aaaaaaaaaa aaaaaa 2096
<210>9
<211>5640
<212>DNA
<213>人
<400>9
ggaaacgcag aaaacagaga gaggcattct gagtcatctg actggatgaa gactgttcca 60
agttacaacc aaacaaatag ctccatggac tttagaaatt atatgatgag agatgagact 120
ctggaaccac tgcccaaaaa ctgggaaatg gcctacactg acacagggat gatctacttc 180
attgaccaca ataccaagac aaccacctgg ttggatcctc gtctttgtaa gaaagccaaa 240
gcccctgaag actgtgaaga tggagagctt ccttatggct gggagaaaat agaggaccct 300
cagtatggga catactatgt tgatcacctt aaccagaaaa cccagtttga aaatccagtg 360
gaggaagcca aaaggaaaaa gcagttagga caggttgaaa ttgggtcttc aaaaccagat 420
atggaaaaat cacacttcac aagagatcca tcccagctta aaggtgtcct tgttcgagca 480
tcactgaaaa aaagcacaat gggatttggt tttactatta ttggtggaga tagacctgat 540
gagttcctac aagtgaaaaa tgtgctgaaa gatggtcccg cagctcagga tgggaaaatt 600
gcaccaggcg atgttattgt agacatcaat ggcaactgtg tcctcggtca cactcatgca 660
gatgttgtcc agatgtttca attggtacct gtcaatcagt atgtaaacct cactttatgt 720
cgtggttatc cacttcctga tgacagtgaa gatcctgttg tggacattgt tgctgctacc 780
cctgtcatca atggacagtc attaaccaag ggagagactt gcatgaatcc tcaggatttt 840
aagccaggag caatggttct ggagcagaat ggaaaatcgg gacacacttt gactggtgat 900
ggtctcaatg gaccatcaga tgcaagtgag cagagagtat ccatggcatc gtcaggcagc 960
tcccagcctg aactagtgac tatccctttg attaagggcc ctaaagggtt tgggtttgca 1020
attgctgaca gccctactgg acagaaggtg aaaatgatac tggatagtca gtggtgtcaa 1080
ggccttcaga aaggagatat aattaaggaa atataccatc aaaatgtgca gaatttaaca 1140
catctccaag tggtagaggt gctaaagcag tttccagtag gtgctgatgt accattgctt 1200
atcttaagag gaggtcctcc ttcaccaacc aaaactgcca aaatgaaaac agataaaaag 1260
gaaaatgcag gaagtttgga ggccataaat gagcctattc ctcagcctat gccttttcca 1320
ccgagcatta tcaggtcagg atccccaaaa ttggatcctt ctgaggtcta cctgaaatct 1380
aagactttat atgaagataa accaccaaac accaaagatt tggatgtttt tcttcgaaaa 1440
caagagtcag ggtttggctt cagggtgcta ggaggagatg gacctgacca gtctatatat 1500
attggggcta ttattcccct gggagcagct gagaaagatg gtcggctccg cgcagctgat 1560
gaactaatgt gcattgatgg aattcctgtt aaagggaaat cacacaaaca agtcttggac 1620
ctcatgacaa ctgctgctcg aaatggccat gtgttactaa ctgtcagacg gaagatcttc 1680
tatggagaaa aacaacccga ggacgacagc tctcaggcct tcatttcaac acagaatgga 1740
tctccccgcc tgaaccgggc agaggtccca gccaggcctg caccccagga gccctatgat 1800
gttgtcttgc aacgaaaaga aaatgaagga tttggctttg tcatcctcac ctccaaaaac 1860
aaaccacctc caggagttat tcctcataaa attggccgag tcatagaagg aagtccggct 1920
gaccgctgtg gaaaactgaa agttggagat catatctctg cagtgaatgg gcagtccatt 1980
gttgaactgt ctcatgataa cattgttcag ctgatcaaag atgctggtgt caccgtcaca 2040
ctaacggtca ttgctgaaga agagcatcat ggtccaccat caggaacaaa ctcagccagg 2100
caaagcccag ccctgcagca caggcccatg ggacagtcac aggccaacca catacctggg 2160
gacagaagtg ccctagaagg tgaaattgga aaagatgtct ccacttctta cagacattct 2220
tggtcagacc acaagcacct tgcacagcct gacaccgcag taatttcagt tgtaggcagt 2280
cggcacaatc agaaccttgg ttgttatcca gtagagctgg agagaggccc ccggggcttt 2340
ggattcagcc tccgaggggg gaaggagtac aacatggggc tgttcatcct tcgtcttgct 2400
gaagatggtc ctgccatcaa agatggcaga attcatgttg gtgaccagat tgttgaaatc 2460
aatggggaac ctacacaagg aatcacacat actcgagcaa ttgagctcat tcaggctggt 2520
ggaaataaag ttcttcttct tttgaggcca ggaactggct tgatacctga ccatggtttg 2580
gctccttccg gtctgtgctc ctacgtgaaa cccgagcaac attaaggctt tcagggcttt 2640
tcttggtctt tccttaaaaa gacttggtga ttgggatatt aataatcctt cgtcttcaaa 2700
tgtgatttat gatgaacagt caccattacc cccatcttca cattttgctt ccatatttga 2760
agagtctcac gtgccagtaa ttgaagaatc tttgagagtt cagatatgtg aaaaggcaga 2820
agaattaaag gacattgtgc ctgaaaagaa aagcacttta aatgaaaatc agcctgagat 2880
aaagcatcag tctcttctcc agaaaaatgt gagtaagagg gatccaccca gcagtcatgg 2940
gcacagtaac aagaaaaatc tattaaaagt agaaaatggt gttacacgaa gaggtagatc 3000
ggttagtccc aaaaagccag ccagtcaaca ttcagaggaa catttggata agattcctag 3060
tcctctaaaa aataacccca aaagaagacc cagagatcaa tccctcagcc ccagcaaagg 3120
ggaaaataaa agttgtcagg tcagcaccag ggcaggctct ggacaagatc agtgcagaaa 3180
aagcagaggt cggtcggcca gcccaaaaaa gcagcaaaaa attgaaggaa gcaaagctcc 3240
atcaaatgct gaggccaaat tattagaggg taagagtcga agaatagcag gctatacggg 3300
cagtaatgct gagcagatcc cagatgggaa ggaaaaatca gacgtcatca ggaaagatgc 3360
aaagcagaat cagttggaaa aaagcagaac aaggtctcca gagaaaaaaa tcaaaagaat 3420
ggttgagaaa tctcttccat ccaaaatgac taataagact acaagtaaag aagtatctga 3480
aaatgaaaaa ggaaagaaag taaccacagg agaaacaagt tctagtaacg ataaaatagg 3540
agaaaatgtc cagctatcag aaaagaggct gaagcaagaa cctgaagaga aggtagtttc 3600
aaacaaaaca gaagatcaca aagggaaaga actagaggca gctgacaaaa acaaagagac 3660
tggaaggttc aaaccggaaa gcagttctcc agttaagaaa acactgataa ctccagggcc 3720
ctggaaggtt ccaagtggaa ataaagtcac aggcactatt ggtatggctg agaaacggca 3780
gtaaccttta gtataaaaca aagaaaaaca agttgtaatc ttttcttaca gcagcatttt 3840
tccagaaaaa gccttttttt ttttttcaga tattctgaaa cagataagta catgttaatg 3900
tgagcctcaa gttacctagg ctgcatgaag ggcctttagg attgctaaga accaactgtc 3960
cccctggccg gctgccctcc ctcgctctca ggaaggagct gcatccacat gctcatctga 4020
cccgccctgc tcaggctgcc cagctcgtct tcatgagtgt ctgaacaaat gacatatgtt 4080
gatattaaca atgtggtcac aactcacttt gtatttgtgc caagttatct actgtatcat 4140
gtctgttttt atcctttttg ttcagctgtt tccacagtaa tgaaaaagtt aggtttggct 4200
tggaagttga tgatctcaat agcatgttgc atgtttacag agagaaatat gtgagtcctt 4260
gcagaagaag agactgttaa ctcatcgtta aagatggccg ttgtctcttc taacagctac 4320
tgatgatgtc ccactttaaa aataaaaccc ccaaacatca ctactttaag gaaaaaaaaa 4380
atgtagtcca atattgatgc tttcttatgg ctttttattt taatttggct ggataagttg 4440
tttcaaataa ctgttaaaga tattacttac aattgaatgt ttgaaataag aaagtacttt 4500
aagcaataga gttcatctcc tgctgtgtta tccaacctcg atgtatactt acagcatctc 4560
aggtcaccct ttttatttca gttatttaat tatgaaacca taaagaagca tgtggaaata 4620
gtgtttattg ctctttgaag aaaaaccacc aactatttct ggatattttg gctgtaccta 4680
ctactaaagt cattagtctt taatacataa tacatatttg aaaagtaaac atattatata 4740
gattatgtga gggacttaat catgaaacca gtttcacagt ccaagtacca actcttctgg 4800
tagcaggtgc acaagcttgg gtgtttaaaa acaacctgtg tagggtatgc ccagcaaatg 4860
aggacaaatg tgtagacagt acttactgga tcttatttaa cttttagcta cattaactaa 4920
ctttcttatt taaaaacaag aaagggagac taaacatctg cttaacttgt acacattttc 4980
agaattcttt ttaaaagtct agttaaagat gtttcttaga agttggagac tgttaacaac 5040
ttccataaaa tagatccagg tttttcagtt ccctgaagca gcattcagta gcatctatat 5100
aaataaaggc accttctgag aataaaacta ttttatggag tgtgtgaaca cacttgttct 5160
gtcacctggg ttcatcttgt tgtgaagcac attaggtcca ggtccttccc tctgggagtc 5220
tgactgtgaa actctttaac ccaacaactc aattagcccc tgtagataag acatgcttcc 5280
cagagtgaga tttttgaaat ccccttttca tccagaacta tatttaccca cctattgtaa 5340
ctattcaaat agagcaaaat taggaggctt gataaatact aagaatttag taccacagaa 5400
attatttatt attttccctg tagtccacaa ttagtgataa cgaatcctat ttttgttaac 5460
tgtgacataa ctttgatgtc atatgttgtc ctatgtggtt cttcctaagt aaactctgta 5520
ctgattatat actgacttag caatgtggcc ttggaatgct gagcaaaatg tggatgtact 5580
ggttgtaaat gtttatatat tgtacagtac ctttatatat acacttgagg ttctgattag 5640
<210>10
<211>457
<212>DNA
<213>人
<220>
<221>misc feature
<222>(242)..(242)
<223>各种碱基
<220>
<221>misc feature
<222>(369)..(369)
<223>各种碱基
<220>
<221>misc feature
<222>(406)..(406)
<223>各种碱基
<220>
<221>misc feature
<222>(457)..(457)
<223>各种碱基
<220>
<221>misc feature
<222>(394)..(394)
<223>各种碱基
<400>10
tcagtcactc tttcaccctg ccaaagcttc actgtcctac tgattgaatt gtatgtgaga 60
aataaaatgt catcatatta agccactggg atttgtatgt ttatctgtta tagcagcaag 120
tcttaattta cctaatacac acattgtgac agatgttctt aatgtcccac cccatattgt 180
tacatgtcca gctttgagga tccctggcat gtgggggtag gagtttctgg gcatgctgga 240
tncaattccc acttttaagg catctgtggc ctctgtggcc tctgtggcct tcactgttat 300
ggaagggatt tatctggggc accataggaa actttaccat ggcacagtgg acaacctagg 360
agggggtgng gaggaggggc cttcaggccc aacngggggg accagngttc gtggggttag 420
ggtggtttgg ggggttttcc ctcttacccg tgggggn 457
<210>11
<211>1493
<212>DNA
<213>人
<400>11
aatagggttg gcggctgcag cgggcggcaa acagcccgcc cggcaccacc atgctcgccc 60
tggaggctgc acagctcgac gggccacact tcagctgtct gtacccagat ggcgtcttct 120
atgacctgga cagctgcaag cattccagct accctgattc agagggggct cctgactccc 180
tgtgggactg gactgtggcc ccacctgtcc cagccacccc ctatgaagcc ttcgacccgg 240
cagcagccgc ttttagccac ccccaggctg cccagctctg ctacgaaccc cccacctaca 300
gccctgcagg gaacctcgaa ctggccccca gcctggaggc cccggggcct ggcctccccg 360
cataccccac ggagaacttc gctagccaga ccctggttcc cccggcatat gccccgtacc 420
ccagccctgt gctatcagag gaggaagact taccgttgga cagccctgcc ctggaggtct 480
cggacagcga gtcggatgag gccctcgtgg ctggccccga ggggaaggga tccgaggcag 540
ggactcgcaa gaagctgcgc ctgtaccagt tcctgctggg gctactgacg cgcggggaca 600
tgcgtgagtg cgtgtggtgg gtggagccag gcgccggcgt cttccagttc tcctccaagc 660
acaaggaact cctggcgcgc cgctggggcc agcagaaggg gaaccgcaag cgcatgacct 720
accagaagct ggcgcgcgcc ctccgaaact acgccaagac cggcgagatc cgcaaggtca 780
agcgcaagct cacctaccag ttcgacagcg cgctgctgcc tgcagtccgc cgggcctgag 840
cacacccgag gctcccacct gcggagccgc tgggggacct cacgtcccag ccaggatccc 900
cctggaagaa aaagggcgtc cccacactct aggtgatagg acttacgcat ccccaccttt 960
tggggtaagg ggagtgctgc cctgccataa tccccaagcc cagcccgggc ctgtctggga 1020
ttccccactt gtgcctgggg tccctctggg atttctttgt catgtacaga ctccctggga 1080
tcctcatgtt ttgggtgaca ggacctatgg accactatac tcggggaggc agggtagcag 1140
tgcttccaga gtcccaagag cttctctggg attttcttgt gatatctgat tccccagtga 1200
ggcctgggac ctttttaaga tcgctgtgtg tctgtaaacc ctgaatctca tctggggtgg 1260
gggccctgct ggcaaccctg agccctgtcc aaggttccct cttgtcagat ctgagatttc 1320
ctagttatgt ctggggccct ctgggagctg ttatcatctc agatctcttc gcccatctat 1380
ggctgtgttg tcacatctgt cccctcattt ttgagatccc ccaattctct ggaactattc 1440
tgctgcccct ttttatgtgt ctggagttcc ccaatcacat ctagggctcc tcc 1493
<210>12
<211>2292
<212>DNA
<213>人
<400>12
ccatgggttc cccttcagcc tgtccataca gagtgtgcat tccctggcag gggctcctgc 60
tcacagcctc gcttttaacc ttctggaacc tgccaaacag tgcccagacc aatattgatg 120
tcgtgccgtt caatgtcgca gaagggaagg aggtccttct agtagtccat aatgagtccc 180
agaatcttta tggctacaac tggtacaaag gggaaagggt gcatgccaac tatcgaatta 240
taggatatgt aaaaaatata agtcaagaaa atgccccagg gcccgcacac aacggtcgag 300
agacaatata ccccaatgga accctgctga tccagaacgt tacccacaat gacgcaggat 360
tctataccct acacgttata aaagaaaatc ttgtgaatga agaagtaacc agacaattct 420
acgtattctc ggagccaccc aagccctcca tcaccagcaa caacttcaat ccggtggaga 480
acaaagatat tgtggtttta acctgtcaac ctgagactca gaacacaacc tacctgtggt 540
gggtaaacaa tcagagcctc ctggtcagtc ccaggctgct gctctccact gacaacagga 600
ccctcgttct actcagcgcc acaaagaatg acataggacc ctatgaatgt gaaatacaga 660
acccagtggg tgccagccgc agtgacccag tcaccctgaa tgtccgctat gagtcagtac 720
aagcaagttc acctgacctc tcagctggga ccgctgtcag catcatgatt ggagtactgg 780
ctgggatggc tctgatatag cagccttggt gtagtttctg catttcggga agagtgtttt 840
tattatccac ctgcagactg gactggattc ttctagctcc ttcaatccca ttttctcctg 900
tggcatcact aagtataaga cctgctctct tcctgaagac ctataagctg gaggtggaca 960
actcaatgta aatttcaagg aaaaccctc atgcctgaga tgtgggccac tcagagctaa 1020
ccaaaatgtt caacaccata actagagaca ctcaaattgc caaccaggac aagaagttga 1080
tgacttcatg ctgtggacag tttttcccaa gatgtcccaa gcctcatcgt gacgaggctc 1140
ttatcccact ccatttttcc ctgctcatgc ctgcctcttt aatttggtaa gataatgctg 1200
taactagaat ttcacaatca gcgccttgtg caggcaattt gacagagtgt tggatgtgtc 1260
atgtcatcat gtcaaaccca aatatttgac ctaagggatc ctttattctg cccagtggct 1320
aactttaaca acatccctaa tacaactgtt tattcaaatg cacggtggtc cctgttagag 1380
ttagacctct agactcacct gttctcacgc cctgttttaa tttaacccag ctatgggatg 1440
ccagataaca gaattgctgc ctacgagctg aacagggagg agtttgtgca gttgctgaca 1500
cttcttgttg cacataaata aatacagtgg gtactataga gactcagttg caaaaattaa 1560
caaatatgct gcttgattaa aatgggtagg cttctcatgt ggctcattct ttaatctatt 1620
ctcttttatt tggtttggtt catggggtct ctgcctatgg atcatacttc aaactcttgg 1680
tgtgatcctc ctgattgtca caatattagt taccctggtg tgctgtattc tctaaaacct 1740
ttaaatgttt gcatgcagcc attcgtcaaa tgtcaaatat tctctctttg gctggaatga 1800
caaaaactca aataaatgta tgattaggag gacatcataa cctatgaatg atggaagtcc 1860
aaaatgatgg taactgacag tagtgttaat gccttatgtt tagtcaaact ctcatttagg 1920
tgacagcctg gtgactccag aatggagcca gtcatgctaa atgccatata ctcacactga 1980
aacatgagga agcaggtaga tcccagaaca gacaaaactt tcctaaaaac atgagagtcc 2040
aggctgtctg agtcagcaca gtaagaaagt cctttctgct ttaactctta gaaaaaagta 2100
atatgaagta ttctgaaatt aaccaatcag tttatttaaa tcaatttatt tatattcttc 2160
tgttcctgga ttcccatttt acaaaaccca ctgttctact gttgtattgc ccagtaggag 2220
ctatcactat attttgcaga atggaaactg ccctgactct tgaatcacaa ataaaagcca 2280
attgtatctg tt 2292
<210>13
<211>519
<212>DNA
<213>人
<220>
<221>misc feature
<222>(212)..(212)
<223>各种碱基
<220>
<221>misc feature
<222>(451)..(451)
<223>各种碱基
<400>13
gaaacaacaa cagtgtaatc tttaacaggg atgttaaagg taagaagtca ggaagataaa 60
ccaaaatgat tgagtatgat aaagaatttt gcatggcgat taaaatagaa aacctataaa 120
tgtagaaaaa gcaggtctgg acttagcaaa gaaacaatat agtttggaga aggcatgaaa 180
taagttcttt tcatgttcac tgctggtcac ancataacag agagtgatgt ggagagcttt 240
gggaaggttt cacgttgagt tacatcagtg gtcaacaatg gagcaacaag actccgtaga 300
ggatgccacc ctgggagaat tgcaagggaa aggaggctga agcacaactg gtaatagcct 360
tcagatattt aatggatatg caaataaagc tctgattaat tgtattttca cttattatat 420
atcatctttg gacctttcta aaagtgggac nctagaaaag atatactgaa actccaaaag 480
aatacttcag ctcgagttga atggattcaa gatgttgtt 519
<210>14
<211>5294
<212>DNA
<213>人
<400>14
ggctcgcatc cccatagtgc tgggttacag tgaaggtacg ccccgcgctc tgctctggag 60
aggcagggtg ggatagggaa cgtctcgagt ggcgcccgca gtcatggtgg tgttcgttgg 120
ccgccgcctc ccggcgctcc tagggctgtt taagaagaag ggctctgcca aggctgagaa 180
tgacaaacat ctaagtgtag ggcctggcca ggggccaggg tctgcagtgg atgagcacca 240
ggacaacgtc ttctttccca gtgggcgacc cccccacctg gaagagctgc acactcaggc 300
ccaggagggg ctccgctccc tacaacacca agagaaacag aaactgaaca agggtggctg 360
ggaccatgga gacacccaga gtatccagtc ctcccggacg gggccggatg aagacaacat 420
ctccttctgc agtcagacca catcctacgt ggctgagagc tccacagcag aggacgcgct 480
ctccatccgc tcggagatga tccagcgcaa aggctccacc ttccgacccc atgactcatt 540
tcccaaatct ggaaagtcag ggcggcgtcg gcgggagcgg cggagcactg tgctgggact 600
cccgcagcat gtgcagaagg agcttggcct gaggaatgag cgtgaggcac caggcacgcc 660
ccgggctcct ggtgcacggg atgccgtacg catccccaca gtggacggcc gcccccgagg 720
cacctcaggg atgggggccc gggtgtccct gcaggcgctg gaggcggagg cagaggctgg 780
cgctgagaca gaggccatgc tgcagcgcca cattgaccgt gtctaccggg atgacacctt 840
tgttggccgg tccacgggta cccgggcccc accattgacc cggcccatgt ccctagcagt 900
gcctggattg acaggagggg cagggcctgc agagcccctg agcccggcca tgtccatctc 960
cccccaggcc acctacctgt cgaagttgat tccacatgct gtgctgccgc ctacagtgga 1020
cgtggtggcc ctaggccgct gcagcctgcg cacactaagc cgctgcagcc tgcactcggc 1080
cagcccagcc tcagtccgct cgctggggcg cttctcctcc gtctccagcc cacagccccg 1140
cagccgccac ccatcctcct ccagtgacac ctggagccac tctcaatcct ccgacaccat 1200
tgtgtctgac ggttccaccc tctcctctaa gggtggctct gagggccagc cggagagctc 1260
tacggctagc aatagcgtgg taccccctcc ccagggaggc agtgggaggg gctctcccag 1320
tgggggcagc actgctgagg cctcagacac actcagcatt cggagcagtg ggcagttgtc 1380
tggccggagt gtgtccctgc gtaagctgaa gcggcctcca ccccctcccc gccggaccca 1440
ctccctccat cagcggggct tagcagtgcc tgatgggcca ttagggttgc cccctaagcc 1500
tgagcgtaag cagcagcccc agctgcctcg gccacccacc actggtggct cagaaggggc 1560
gggggcagca ccctgtccac ccaacccagc caacagctgg gtacctggct tgtctccggg 1620
tggttcccgg cgccccccac ggtccccaga acggacactt tcgccctcca gtggatactc 1680
gagccaaagt ggtactccca ccctccctcc caagggcctg gcaggtcccc ctgcttcccc 1740
aggcaaggcc cagcccccta aaccagagcg tgtcacgtct cttcgctccc ctggggcctc 1800
cgtctcctct tccctcacgt ctttatgttc ctcctcctct gacccagccc cctcagaccg 1860
ctctgggcca cagatattga cccccctggg tgacaggttt gtcatacctc ctcaccccaa 1920
ggtgcctgcc cccttctccc cacctccctc caagcccagg agccctaacc cagctgcccc 1980
tgctctagcc gcccctgctg tggttcctgg gcctgtttct accactgacg ccagtcctca 2040
gtcccctccc actccccaga caaccttgac tccactgcag gagtctcctg tcatctccaa 2100
agaccagtca cccccacctt ccccaccccc atcttatcat ccacccccac cacccactaa 2160
gaagccagag gtggttgtgg aggcaccatc tgcctcagag actgctgagg agcccctcca 2220
agatcccaac tggccccctc ccccaccccc tgcccctgag gagcaggacc tgtccatggc 2280
tgacttcccc ccaccagagg aggctttttt ctctgtggcc agccctgagc ctgcaggccc 2340
ttcaggctcc ccagagcttg tcagctcccc ggctgcttcg tcctcctcag ctactgcttt 2400
gcagattcag cccccgggta gcccagaccc tcctccagct ccgccagccc cagctcctgc 2460
tagttccgcc ccagggcatg tggccaagct ccctcagaag gaaccggtgg gctgtagcaa 2520
gggtggtggg cctcccaggg aggacgtagg tgcgcccctg gtcacgccct cgctcctgca 2580
gatggtgcgg ctgcgctccg tgggtgctcc aggaggggct cccaccccag cactggggcc 2640
atcggccccc cagaaaccac tgcgaagggc cctgtcaggg cgggccagcc cagtgcctgc 2700
cccctcctca gggctccatg ctgcggtccg actcaaggcc tgcagcctgg ccgccagtga 2760
aggcctctca agtgctcagc ccaacggacc gcctgaggca gagccacggc ctccccagtc 2820
ccctgcctca acggccagtt tcatcttctc caagggctct aggaagctgc agctggagcg 2880
gcccgtgtcc cctgagaccc aggctgacct ccagcggaat ctggtggcag aactccggag 2940
catctcagag cagcggccac cccaggcccc aaagaagtca cctaaggctc ccccacctgt 3000
ggcccgcaag ccgtctgtgg gagtcccccc acccgcctcc cccagttacc ctcgagctga 3060
gccccttact gctcctccca ccaatgggct ccctcacacc caggacagga ctaagaggga 3120
gctggcggag aatggaggtg tcctgcagct ggtgggccca gaggagaaga tgggcctccc 3180
gggctcagac tcacagaaag agctggcctg accaccaggc acctcactgg cactgctgac 3240
ccatcccaga aacacaatct cagggacccg agcagctcca aggacgagag gatacagcag 3300
acacaaccta atagagaggg cgcctgcagc cttaacctcc acggccttcg atacttatgc 3360
aagcctggtg ttgctcctgt cctcagagtc atcctgcgct catgcctttt cccgaatggg 3420
ttcacctctg gcagttgccg cttcagtctt ggccttagcc tcatcttgaa gtgggtagct 3480
ggcgggagag ggtggctgcg ccccctgctg gccctgaggc tgcagagttg ggagcaggac 3540
acctcacctg agtttcattt tttttcatgt ccaaaccatg cacatactat agtccagaat 3600
caaagcactt ttgaaaagtg gctgcatggc catcctccag ggcccaggaa gttgcattcc 3660
aagggcctgt ttacatggca gcagaatcca tccccggcag tcagcccata gcttgggacc 3720
agtctgtgcc ctcctgccca gtccagttta ctcctcttgg ttcctgaagg tggccaagtc 3780
attgtgttcc cacaggcttc tctaggctgg gggcaggtgt ggggctgtgg aattccaaag 3840
cacaaaaggt gcagagggga ttggccttcc tgtgcctcaa ctcaccaacc accctcctgc 3900
cttccagttc tgccaggtgc tccatgctgg ggacaagtag gagactgcca gggcccaaag 3960
aaatgggtga gcagtagagt catctcgggg cacttggcag tgtcaagcac ctgccccttg 4020
cctccttgac cacactgggg tgggtgggcc cccagcactt cagaggcagg agcctttggg 4080
ctgagcaagc actgaggagg tggatggaag ggagcatctg gaggggggga gcttccttga 4140
gcagtgggcc caggcctggc cctccacact tcattctctg acctttctct ctcctcattt 4200
cggtgcatgt cctttctgca gctgcctttc agcacaggtg gttccactgg gggcagctaa 4260
cgctgagtga caaggatggg aagccacagg tgcattttac tcaagtcttc tctagtcaat 4320
gaggggcacc cagtgcttct agggcaggct gggtggtggt cccctaggta tcagcctctc 4380
ttactgtact ctccgggaat gttaaccttt ctattttcag cctgtgccac ctgtctaggc 4440
aagctggctt ccccattggc ccctgtgggt ccacagcagc gtggctgccc cccagggcca 4500
ccgcttcttt cttgatcctc tttccttaac agtgacttgg gcttgagtct ggcaaggaac 4560
cttgctttta gcttcaccac caaggagaga ggttgacatg acctccccgc cccctcacca 4620
aggctgggaa cagaggggat gtggtgagag ccaggttcct ctggccctct ccagggtgtt 4680
ttccactagt cactactgtc ttctccttgt agctaatcaa tcaatattct tcccttgcct 4740
gtgggcagtg gagagtgctg ctgggtgtac gctgcacctg cccactgagt tggggaaaga 4800
ggataatcag tgagcactgt tctgctcaga gctcctgatc taccccaccc cctaggatcc 4860
aggactgggt caaagctgca tgaaaccagg ccctggcagc aacctgggaa tggctggagg 4920
tgggagagaa cctgacttct ctttccctct ccctcctcca acattactgg aactctatcc 4980
tgttaggatc ttctgagctt gtttccctgc tgggtgggac agaggacaaa ggagaaggga 5040
gggtctagaa gaggcagccc ttctttgtcc tctggggtaa atgagcttga cctagagtaa 5100
atggagagac caaaagcctc tgatttttaa tttccataaa atgttagaag tatatatata 5160
catatatata tttctttaaa tttttgagtc tttgatatgt ctaaaaatcc attccctctg 5220
ccctgaagcc tgagtgagac acatgaagaa aactgtgttt catttaaaga tgttaattaa 5280
atgattgaaa cttg 5294
<210>15
<211>988
<212>DNA
<213>人
<400>15
gtcgtgaggc gggccttcgg gctggctcgc cgtcggctgc cggggggttg gcctgggtgt 60
cattggctct gggaagcggc agcagaggca gggaccactc ggggtctggt gtcggcacag 120
ccatggcggg cgcgttggtg cggaaagcgg cggactatgt ccgaagcaag gatttccggg 180
actacctcat gagtacgcac ttctggggcc cagtagccaa ctggggtctt cccattgctg 240
ccatcaatga tatgaaaaag tctccagaga ttatcagtgg gcggatgaca tttgccctct 300
gttgctattc tttgacattc atgagatttg cctacaaggt acagcctcgg aactggcttc 360
tgtttgcatg ccacgcaaca aatgaagtag cccagctcat ccagggaggg cggcttatca 420
aacacgagat gactaaaacg gcatctgcat aacaatggga aaaggaagaa caaggtcttg 480
aagggacagc attgccagct gctgctgagt cacagatttc attataaata gcctccctaa 540
ggaaaataca ctgaatgcta tttttactaa ccattctatt tttatagaaa tagctgagag 600
tttctaaacc aactctctgc tgccttacaa gtattaaata ttttacttct ttccataaag 660
agtagctcaa aatatgcaat taatttaata atttctgatg atgttttatc tgcagtaata 720
tgtatatcat ctattagaat ttacttaatg aaaaactgaa gagaacaaaa tttgtaacca 780
ctagcactta agtactcctg attcttaaca ttgtctttaa tgaccacaag acaaccaaca 840
gctggccacg tacttaaaat tttgtcccca ctgtttaaaa atgttacctg tgtatttcca 900
tgcagtgtat atattgagat gctgtaactt aatggcaata aatgatttaa atatttgtta 960
aaaaaaaaaa aaaaaaaaaa aaaaaaaa 988
<210>16
<211>4908
<212>DNA
<213>人
<400>16
ggataacctc gcagggtggg ccggagggcg ggcgccgccg ctgcctgtgc tgcggcgatg 60
gcccagtgtg tacaatcagt gcaggagcta atcccggact ccttcgtccc ctgtgtcgct 120
gcgctgtgca gcgacgaagc cgagcggctc actcgtctca atcacctcag cttcgcggag 180
ctgcttaagc ccttctcccg cctcacttcc gaggttcaca tgagagatcc taataatcaa 240
cttcacgtaa ttaaaaattt gaagatagca gtaagcaaca ttgtcaccca gccacctcag 300
cctggagcca tccggaagct tttgaatgat gttgtttctg gcagtcagcc tgcagaagga 360
ttagtagcta atgtgattac agcaggagat tatgacctta acatcagtgc cactactcca 420
tggtttgagt cttacagaga aacctttctt cagtcgatgc cagcatcgga tcatgaattt 480
ctgaaccact atttagcatg tatgttggta gcgtcatcta gtgaagctga acctgtggaa 540
cagttttcaa agttgtcaca agaacagcat cgaattcagc acaacagtga ttattcctac 600
cccaagtggt ttataccaaa tacacttaaa tactatgtac ttttacatga tgtaagtgca 660
ggagatgaac agagagctga atcaatttat gaagaaatga aacagaaata tggaactcag 720
ggttgctatt tacttaaaat taattctcga acatctaatc gagcatcaga tgaacagata 780
ccagatcctt ggagtcagta tctccagaaa aatagtattc aaaaccagga atcatatgaa 840
gatggccctt gtactataac ttcaaataag aattctgata ataacttgct ttcattggat 900
ggattagata acgaagtcaa agatggctta ccaaataact ttagagctca cccacttcag 960
ttggagcaat ccagtgaccc ttctaacagt attgatggcc cagatcatct aagatctgct 1020
tcatcgttac atgaaacaaa gaaaggaaat actggaataa ttcatggtgc atgtttaaca 1080
cttactgatc atgatagaat tcgacagttt atacaagagt tcacatttcg gggccttttg 1140
ccacatatag agaaaacaat taggcaatta aacgatcagc taatatcaag aaaaggtttg 1200
agtcgatctc tattttctgc aactaaaaaa tggtttagtg gcagtaaagt tccagaaaag 1260
agcattaatg acctgaaaaa tacatctggc ttgctgtatc ctccggaagc accagaactt 1320
caaatcagga aaatggctga cttatgtttt ttggtgcagc attatgattt ggcttacagt 1380
tgctatcata ctgcaaagaa agattttctt aatgatcaag caatgcttta tgcagctggt 1440
gccttggaaa tggcagcagt gtctgctttt cttcaaccag gagcacctag gccatatcct 1500
gctcattaca tggatacagc aattcagaca tacagagata tctgcaagaa tatggtgttg 1560
gctgaaagat gtgtgttgct tagtgctgaa cttttaaaaa gccaaagcaa atattcagag 1620
gctgcagctc tcctaatacg gttgaccagt gaggattctg atcttcgaag tgcacttctt 1680
ttggaacagg cagcacattg ctttataaac atgaaaagtc ccatggttag aaaatatgca 1740
tttcatatga tattggcagg ccatcgattt agtaaagcag ggcagaaaaa gcatgcttta 1800
cgctgttatt gtcaagccat gcaagtttac aaaggaaaag gctggtctct tgcagaggat 1860
cacattaatt tcactattgg gcgccagtcc tatactctta gacagctgga taatgctgtg 1920
tctgctttta ggcatattct aattaatgaa agtaaacaat ctgctgctca acagggggct 1980
ttcctcagag aatatcttta tgtttacaag aatgtaagtc agctgtcacc agatggtcct 2040
ttgccacagc ttcctttacc gtatattaac agttcagcaa cacgggtttt ttttggccat 2100
gacagacgac cagcggatgg tgaaaaacaa gcagctactc atgtaagtct tgatcaagaa 2160
tatgattctg aatcctctca gcagtggcga gaacttgagg aacaagttgt ttctgtggtt 2220
aacaaaggag taattccatc caattttcat cccacacaat actgtttgaa cagttactca 2280
gataattcaa gatttccact tgcagttgta gaagaaccaa ttacagtgga agtggctttt 2340
agaaaccctt tgaaagttct acttttgttg actgatttgt cattgctttg gaagtttcat 2400
cctaaagatt tcagtggaaa ggataatgaa gaagttaaac aactagttac aagtgaacct 2460
gaaatgattg gagctgaagt tatttcagag ttcttaatta atggcgaaga atcaaaagtg 2520
gcaagactaa agctctttcc ccatcacata ggggagctgc atattctggg agttgtttat 2580
aatcttggca ctattcaggg ctctatgaca gtagatggca ttggtgctct tcccggatgt 2640
cacacaggaa aatattcctt gagtatgtca gtccgaggga agcaggattt agaaattcaa 2700
ggtcctcgac ttaacaacac aaaagaagag aaaacatctg ttaaatatgg ccctgatcga 2760
cgtttagatc ccataatcac agaagaaatg ccactgttgg aggtgttctt tatacatttt 2820
cctacagggc ttctctgtgg agaaatccga aaagcatatg tagaatttgt caatgtcagc 2880
aaatgtccac ttactggatt gaaggttgtt tctaaacgtc cagagttctt tactttcggt 2940
ggtaatactg ctgttctaac accactaagt ccctcagctt ctgagaattg tagtgcttac 3000
aagactgttg tgacagatgc tacctctgtg tgtacagcac tcatatcatc agcttcttct 3060
gtagactttg gcattggcac aggaagtcaa ccagaggtga ttcctgttcc ccttcctgac 3120
actgttcttc tacccggagc ctcagtgcag ctgccaatgt ggttacgtgg gcctgatgaa 3180
gaaggtgtcc atgaaattaa ctttttgttt tactatgaaa gtgtcaaaaa gcagccaaaa 3240
atacggcaca gaatattaag acacactgca attatttgta ccagtcggtc tttaaatgta 3300
cgggccactg tctgcagaag taattctctt gaaaatgaag aaggcagagg aggcaatatg 3360
ctagtctttg tggatgtgga aaataccaat actagtgaag caggcgttaa ggaattccac 3420
atagtgcaag tatcaagtag tagcaaacac tggaagttac agaaatctgt aaatctttct 3480
gaaaacaaag atgccaaact tgccagtagg gagaagggaa agttttgctt taaggcaata 3540
agatgtgaga aagaagaagc ggccacacag tcctctgaaa aatatacctt tgcagatatc 3600
atctttggaa atgaacagat aataagttca gcaagcccat gtgcagactt cttttatcga 3660
agtttatctt ctgaattgaa aaaaccacaa gctcacttgc ctgtgcatac agaaaaacag 3720
tcaacagagg atgctgtgag attgattcaa aaatgcagtg aggtagattt gaatattgtc 3780
atattatgga aggcatacgt tgtggaagac agtaaacagc ttattttgga aggtcaacat 3840
catgttattc ttcgcactat aggaaaagaa gccttttcat atcctcagaa acaggagcca 3900
ccagaaatgg aactattgaa atttttcagg ccagaaaaca ttacagtttc ctcaaggcca 3960
tcagtagagc agctttctag tctcattaaa acgagtcttc actacccaga atcatttaat 4020
catccatttc atcaaaaaag cctttgttta gtaccagtca ctcttttact ttccaattgt 4080
tctaaggctg atgtagatgt catagttgat cttcggcata aaacaacaag tccagaagca 4140
ctggaaatcc atggatcatt cacatggctt ggacaaacac agtataaact tcaacttaaa 4200
agccaggaga ttcacagtct gcagctgaaa gcatgctttg ttcatacagg tgtttataac 4260
cttggaactc ctagggtatt tgccaagtta tcggaccaag ttacagtgtt tgaaacaagt 4320
cagcagaatt ccatgcctgc cctgatcatc atcagtaatg tgtgacaact tggaaatttg 4380
tactgaaatc cacaataatc agtttttgct ggatgggttt tacagcagta tttgatatac 4440
ctaacttgtt atggaggttg attgatatct gatccctgca aaatactttg acttgtcatt 4500
ttgttgatga tgcaaagcac gttggactga gaatacttaa cattcttttt ctgtatttct 4560
ttaaaccctg agaataattt acatgctcat aatacaggat atcagcatat ttgtgcacct 4620
tattaagccc catcttaaga aaacacaaag tctaagtctg ctgttacaac ttgtcaatgg 4680
tatacgaata ttaggagatg attctgagaa aggaaaggcc ttgttggcag tactcctgtt 4740
aagccattag tctctaaatt ccagctttac tgtgaagttc tatagagtgt taaatacaaa 4800
ttttcctgtc ttgcttcaca cagttcctta aaatcagttt tgaactttgg tcatagagtc 4860
ttcatatttc agtatttggt ggtccctatg acttatacat aactttgt 4908
<210>17
<211>435
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(30)..(30)
<223>各种碱基
<220>
<221>misc_feature
<222>(49)..(49)
<223>各种碱基
<220>
<221>misc_feature
<222>(75)..(75)
<223>各种碱基
<220>
<221>misc_feature
<222>(76)..(76)
<223>各种碱基
<220>
<221>misc_feature
<222>(78)..(78)
<223>各种碱基
<220>
<221>misc_feature
<222>(79)..(79)
<223>各种碱基
<220>
<221>misc_feature
<222>(109)..(109)
<223>各种碱基
<220>
<221>misc_feature
<222>(136)..(136)
<223>各种碱基
<220>
<221>misc_feature
<222>(137)..(137)
<223>各种碱基
<220>
<221>misc_feature
<222>(149)..(149)
<223>各种碱基
<220>
<221>misc_feature
<222>(227)..(227)
<223>各种碱基
<220>
<221>misc_feature
<222>(236)..(236)
<223>各种碱基
<220>
<221>misc_feature
<222>(246)..(246)
<223>各种碱基
<220>
<221>misc_feature
<222>(342)..(342)
<223>各种碱基
<220>
<221>misc_feature
<222>(363)..(363)
<223>各种碱基
<220>
<221>misc_feature
<222>(389)..(389)
<223>各种碱基
<220>
<221>misc_feature
<222>(426)..(426)
<223>各种碱基
<400>17
ggtagaaatg attgtgatgt acaaattttn tattttgatc atacttaana agacagagca 60
gactcacatt cattnncnna atagtatcac tgtacacata gcgaatttnt ggcgctttta 120
gattgctctg aaaatnnctg aagagttgnc catagcagcc tggtaagcct tttcctttcc 180
cccaaagctc tcctgccctt tgcagaaaga ctgttggtga caactgntgc taactnaata 240
gcatgnggtt gaacttcgcc aaaatccttc cacctcctcc catagggcaa caggggtgac 300
ttgggcttaa agggcattga gtaagcaagt aggttatcag anaacagagg gaagattcca 360
ttntagataa tttccaaata ttacaattng tggaactcag agttcaactg ctcagttcct 420
tcttcngctg accct 435
<210>18
<211>2224
<212>DNA
<213>人
<400>18
ctttagatct gtgcagcctt tgcgtgccaa acttgtgaaa ttccttttac cttttttgga 60
gtacttgcta taaagccacc tgtcaacaaa cccccattat gtacagaata ggacctatcc 120
agtagccagg ccagtaggca gttggggaag gtgggaagga tccagcgagg cccctgagcc 180
tgcacctgga caggtgtacg tctgcaccca tcaccctcag caccaggcca ccctgcagtc 240
cacttactgt actgtgttgt ggaaggatat gctaagtgat gaaagttgcg agcagtctca 300
ctggtcgtgt aaactttttt ttttttttgg aaattgaagc tgtagagtgc tgcccgaaat 360
ctctaggaag ttggtggcaa gggacagcac tcacactctt ctggtcatga tctctgatct 420
ccacctcaaa tgacaataaa aaactggtcc aacgaagaca ctgctcagca cttcagccat 480
caggactaat ccatcgatga ctggaaaaga ggctagcttt gaggaaaaca gcctgggctc 540
ttgggagcag agtccagtgg gtgtgaggct gacttgccga cggtcggcag gtaatggctc 600
tcagccggcg aggcggtccc acagctctcc tcccagggca gcctgaggag gaggaggccg 660
ggtgcctgtt tggtggcagc ttcagcctag ggatacctga agctgttgag caacaccttt 720
atgaaatgtt gccagagcag caacacttcc ctgtgggcac agccccggga aatccggtac 780
caagtgagca aggtggcagg acccacccaa gcctgatacg catctgggcc cgccgggctc 840
agcaggggag gctgctacgg ctgcccactt cccagcaccg tctgtcaggc ttgaacccct 900
ctgtgctgtt cccttcctgg ctaataggga gacccttcgc aggcacccac tgtttcaact 960
tgaccctccc accccctgct actctcctcc acacacccct ccgttccgct agcctaccct 1020
gtcagccttt caataaaagt tatgcacaaa tgtgaacacc tgagatggag ctgaacattt 1080
cttcactttg ttctttttct gaagtcaaac tcttatcaaa tgccctaaaa ttattaccac 1140
ccaagagaaa caggaaaaag gttacatgtt tttgtttact gagagtaaga tcacctgcat 1200
ctggaagacg ggctggtaaa ttggtttggc tacagaacag aaagaaaaca aaaacaaacc 1260
tcgtaaggga agtatcgcac tcagacacca ccacttccta gagccaaatg agcaatccca 1320
aactgcaagt gccgtaagtg ggcctgtgac gtcacaccgc ccggcccgag gtatcgcatg 1380
tgcgggggag gcccacacta cagctgtcct ctcgtctaga aggcaccacc tcgctttcat 1440
gtcccgtgtg ttttggaaaa gcagtatggt gtgtcatgtc tagcggcgaa cacttccctc 1500
cctctgtcct tgaggttgta atataaaaac tgtgtttctg tacgtgtggg tgggaattct 1560
ctgacggtgc tcgttcatag cacaagctta cgctgagttc tgaactgtcg ttcacagctg 1620
cgtgtctgca tggtgtcgca tctgttgtac ctttggggaa aatttgtatg taaatgtaca 1680
gaaataaaaa cgttgcccca ttaacagatt tcctctggaa tgtcttccct acctcacctg 1740
atggtatcca ccgaagggca tttcactacc attaatggtg agtaataaaa tcctccgtgt 1800
tcattcagac ctcactgcgt cactactttg aacgcctctg taagctgtgt cttcacccgc 1860
cccgaggtgg gtggagggag gcctctcact ctgcttcgag tcctggtctt aaaggtagtc 1920
agaggcagag gctggattaa acacacactg tttaccaagt gccactctca gaccacctga 1980
gagacggggg gccatcagta aaattaagag gaattttttt cccttgttcg tgtatgttct 2040
gctgatccgt ggcctgaagg ttcctagaga cgtcaagaaa tgaatatctt acactgtgat 2100
tctgtgagga aagactggta acccaaaact ctcttctcta atgtattttt taacgaaaat 2160
gacaatattt ctttaataaa gtatttatac caaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2220
aaaa 2224
<210>19
<211>2244
<212>DNA
<213>人
<400>19
gttgtttaaa agcaaggcat gcttgtggat gactctgtaa cagactaatt ggaattgttg 60
aagctgctcc ctggttccac tctggagagt aatctgggac atcttagtgt tttgttttgt 120
ttttttccct cctctttttt tgggggggag tgtgtgtggg gtttgttttt tagtcttgtt 180
tttttaattc attaaccagt ggttagcctt aaggggagga ggacggattg attccacatt 240
ccacttccta gatctagttt agaaaacatg ttccccatct ggtgctctta ggaaggagta 300
tagtaaatgc ctcatttaat aacatactcc tttttgaaag ttgccttttc tctccaccct 360
tgagtagatc cagtatttga tgaaactcat gaaagtgggt ggagcccatc ttccccctcc 420
tcttttctag gacgcactat atgtgactgt gactttaagg acatttgttt gccatttgct 480
gatttttttg ggaagttaat ttctaacttc tttcactgat aaatgaagaa aagtattgca 540
cctttgaaat gcaccaaatg aattgagttt gtaattaaaa aaattttttt tccctttcag 600
tcattgtctt atatgcttag catagatttg cagctcagta gtatatgtgt tcctagaatg 660
cagctgaaga cctgttatgt agaggaaata cgaggggtgg tgctagaaga cagacatctg 720
tggaatgatt cacatcctct caagttagga ggatggaggc ctgcttcatt aagaagctgg 780
gggtagggtg ggggtgggga gaacacttaa caacatgggg accagtcagg ggaatcccct 840
tatttctgtt ttgcatatga ggaaccctag agcagccagg tgaggctctc tagtttaata 900
aaaatcatgg aaagactctt aatgcagact cttcttaagt gttaataggg attttttcag 960
cttattttgg ttgcagtttc caatttttaa aaatgttgag gtaatctttc ccaccttccc 1020
aaacctaatt cttgtagatg cattagtgtt gaaccaatgc ttctcatgtc tcaatcttgt 1080
atatcatctt ttcagatgta ttaacaaaca aaaccttaaa aagagtagat gaattgccaa 1140
acacaattcc taccaataat aaatcgatca actctatcta ttcaggaaag caggaagcat 1200
ttggaccaca gtgcatgaaa acttcaacat tctgttatta gataatgaat caaccaaatg 1260
aacaatccag agaaaagaaa attgcaataa taaaaggtaa attaacagaa agataatata 1320
agcaagatag taatagttga ccattctgaa aagcttataa catcactcat catccagcat 1380
cctttctgaa aacaaaggat ttttaaatca ctttatgcac atatacaaca taggaggttg 1440
gcaaaataat gcactatttc ttaacagcca tgtctcttgt agaacttcaa gttaatctac 1500
aaatgaccat tgtgtcttaa tttagattat gaataccaca ttagtcaggt atttgcacta 1560
acccttaata gtatatacag tttctatgga aaattcagtg gtccaaaaat ttccgtagaa 1620
tttgagagga cgttggtggg ctgaagatag ctccttgagg gtcactgatg taggctgcaa 1680
tgggggttca caaggccctg acaccgtatt tatagtctaa cctttttatg aaaatctgac 1740
tacagctatt taaggagtag tcttaatagc tgaaaatgaa gatagagaaa gacaccaaga 1800
atatgacaca gtttacattc tagtgaggga cacaacaaaa tcaaatttaa aaaagagtgt 1860
aatagatgct gataaatact gtagataaag cacataagaa aatagaaata aaggctgtca 1920
atggagaagt catgattttt attttattta tttatttatt tatttgagac agagtcaggc 1980
tctgtgcagg ctggagtgca atggtgtgat ctcgctcact acaacctctg ctcctggctc 2040
aagctatcct cccacctcag ctctcaagta gctgggatca caggtgcgtg ctaccatgcc 2100
cggctaattt tttgtagaga tgaggttttg ccatgttgcc caggctggtc tcgaactcct 2160
ggactcaact gaccccacct cggcctctca aagtgctgag attataggcg tgcagccggc 2220
agctggccat tgtttatgtt ctgc 2244
<210>20
<211>351
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(62)..(62)
<223>各种碱基
<220>
<221>misc_feature
<222>(121)..(121)
<223>各种碱基
<220>
<221>misc_feature
<222>(207)..(207)
<223>各种碱基
<220>
<221>misc_feature
<222>(220)..(220)
<223>各种碱基
<220>
<221>misc_feature
<222>(276)..(276)
<223>各种碱基
<220>
<221>misc_feature
<222>(300)..(300)
<223>各种碱基
<220>
<221>misc_feature
<222>(315)..(315)
<223>各种碱基
<220>
<221>misc_feature
<222>(336)..(336)
<223>各种碱基
<400>20
tctacttcca catcggcgag accgagaagc gctgtttcat cgaggaaatc cccgacgaga 60
cnatggtcat cggcaactat cgtacccaga tgtgggataa gcagaaggag gtcttcctgc 120
nctcgacccc tggcctgggc atgcacgtgg aagtgaagga ccccgacggc aaggtggtgc 180
tgtcctggca gtacggctcg gagggcnctt tcacgttcan ctcccacacg cccggtgacc 240
atcaaatctg tctgcactcc aattcttacc aggatngctc tctttcgctg gtgggcaaan 300
tgcgtgttgc atctngacat ccaggtttgg gggagnatgc caacaaatta c 351
<210>21
<211>2631
<212>DNA
<213>人
<400>21
accttccaac ccagccctcg gctgagccgc gccgcaccat gcccgccgtg gacaagctcc 60
tgctagagga ggcgttgcag gacagccccc agactcgctc tttactgagc gtgtttgaag 120
aagatgctgg caccctcaca gactatacca accagctgct ccaggcaatg cagcgcgtct 180
atggagccca gaatgagatg tgcctggcca cacaacagct ttctaagcaa ctgctggcat 240
atgaaaaaca gaactttgct cttggcaaag gtgatgaaga agtaatttca acactccact 300
atttttccaa agtggtggat gagcttaatc ttctccatac agagctggct aaacagttgg 360
cagacacaat ggttctacct atcatacaat tccgagaaaa ggatctcaca gaagtaagca 420
ctttaaagga tctatttgga ctcgctagca atgagcatga cctctcaatg gcaaaataca 480
gcaggctgcc taagaaaaag gagaatgaga aggtgaagac cgaagtcgga aaagaggtgg 540
ccgcggcccg gcggaagcag cacctctcct cccttcagta ctactgtgcc ctcaacgcgc 600
tgcagtacag aaagcaaatg gccatgatgg agcccatgat aggctttgcc catggacaga 660
ttaacttttt taagaaggga gcagagatgt tttccaaacg tatggacagc tttttatcct 720
ccgttgcaga catggttcaa agcattcagg tagaactgga accgaggcgg aaaagatgcg 780
ggtgtcccag caagaattac tttctgttga tgaatctgtt tacactccag actctgatgt 840
ggccgcacca cagatcaaca ggaacctcat ccagaaggct ggttacctta atcttagaaa 900
caaaacaggg ctggtcaccg ccacctggga gaggctttat ttcttcaccc aaggcgggaa 960
tctcatgtgt cagcccaggg gagccgtggc tggaggtttg atccaggacc tggacaactg 1020
ctcagtgatg gccgtggatt gcgaagaccg gcgctactgc tttcagatca ccacgcccaa 1080
tggaaaatcg ggaataatcc tccaggctga gagcagaaag gaaaatgaag agtggatatg 1140
tgcaataaac aacatctcca gacagatcta cctgaccgac aaccctgagg cagtcgcgat 1200
caagttgaat cagaccgctc tgcaagcagt gactcctatt acaagttttg gaaaaaaaca 1260
agaaagctca tgccccagcc agaacctgaa aaattcagag atggaaaatg aaaatgacaa 1320
gattgttccc aaagcaacag ccagtctacc tgaagcagag gagctgatcg cgcctggagc 1380
gccgattcaa ttcgatattg tgcttcctgc tacagaattc cttgatcaga acagagggag 1440
caggcgtacc aacccttttg gtgaaactga ggatgaatca tttccagaag cagaagattc 1500
tcttttgcag cagatgttta tagttcggtt tttgggatca atggcagtta aaacagacag 1560
cactactgaa gtgatttatg aagcgatgag acaagtattg gctgctcggg ctattcataa 1620
catcttccgc atgacagaat cccatctgat ggtcaccagc caatctttga ggttgataga 1680
tccacagact caagtatcaa gggccaattt tgaacttacc agtgtcacac aatttgctgc 1740
tcatcaagaa aacaagagac tggttggttt tgtcatccgt gttcctgaat ccactggaga 1800
agaatctctg agtacataca tttttgaaag caactcagaa ggcgaaaaga tatgttatgc 1860
tattaatttg ggaaaagaaa ttattgaggt tcagaaggat ccagaagcac tggctcaatt 1920
aatgctgtcc ataccactaa ccaatgatgg aaaatatgta ctgttaaacg atcaaccaga 1980
tgacgatgat ggaaatccaa atgaacatag aggcgcagaa tccgaagcat aactcacttg 2040
cgcctgtggg ggaagagcga acaggaagga gagctacctc ctaagggttt taacgtctct 2100
gacatacagg cacactgacc tgatttccga aggctgacaa tcgtttgtgg aatgtaatct 2160
tgatgccttg atactgagac ttgggaggga aactaagaaa tggttgacag cgttcccacc 2220
catctacaat gttattttag gtgctttgtg gtaagtcttt tttcttagat tgcgctaaaa 2280
tttcttagat tgttcagcgc tcagaacaaa agtttgaaaa atgcattgtt catatgaatg 2340
tcatctcttt tcagtttcca gtatcctttt taaaaaatgg caaaagccta gatttacaat 2400
ttgatgaaca ctaaatattt cttattaata taatctattt ttgtatttta cttaatgagc 2460
tttaagtgcc tgtcgttctg aaaattgtgt atttataatt cagcttatct cataattgga 2520
cctaatagca tttctttgtg cagttaggtg atgagcactg ctttgaggcc caagcactag 2580
tagagatgcg cgatacaggt ctagtttcgg taactgttcc agacatcaag c 2631
<210>22
<211>2851
<212>DNA
<213>人
<400>22
agcatctcag gccatcatcc tgaaacttgg cagccttcgt ggagtataag gacagcatta 60
ttagccatca ttgggtttac tgccaacaaa aggagaggga gccataggtt ctctagatta 120
cactcctgag gaaagaagag cacttgccaa aaaatcacaa gatttctgtt gtgaaggatg 180
tggctctgcc atgaaggatg tcctgttgcc tttaaaatct ggaagcgatt caagccaagc 240
tgaccaagaa gccaaagaac tggctaggca aataagcttt aaggcagaag tcaattcatc 300
tggaaagact atctctgagt cagacttaaa ccactctttt tcactaactg atttacaaga 360
tgatatacct acaacattcc agggtgctac ggccagtaca tcgtacggac tccagaattc 420
ctcagcagca tcctttcatc aacctaccca acctgtagct aagaatacct ccatgagccc 480
tcgacagcgc cgggcccagc agcagagtca gagaaggttg tctacttcac cagatgtaat 540
ccagggccac cagccaagag acaaccacac tgatcatggt gggtcagctg tactgattgt 600
catcctgact ttggcattgg cagctcttat attccgacga atatatctgg caaacgaata 660
catatttgac tttgagttat aatatggttt tgtgacttat gagctgtgac tcaactgctt 720
cattaaacat tctgcattgg gtataatcta agaattgttt acaaaaagat tattttgtat 780
ttacccttca ttcctttttt tgatccttgt aagtttagta taaatatatc tagacattca 840
gactgtgtct agcagttacg tcctgcttaa agggactaga agtcaaagtt ccttgtctca 900
ctatttgatc tgctttgcag ggaaataact tgttttttct catgtttcat cttcttttta 960
tgtaaatttg taatactttc ctatattgcc ctttgaaatt tttggataaa agatgatgtt 1020
ttaagttcca atgagtatta ctagttactc aataccactt attgagtact ctgtttctac 1080
gtatgtagaa tgtataggga tagaagagtt gaaaagggaa agcaaaactt cttaagtggc 1140
ttccttaaaa tgtcattcat aggagatgta ctggaattgc tcattctgtg actttatttg 1200
tgtcctaaac attcttcagt gaaaataatt ttatttcagt caaacattta tgaggaaatg 1260
agatcacatc tttgtcactg gatgctactt gaagagggag tactttgtaa ccactttgat 1320
atgctgttat caccaccccc tgccctctgc tgccataatc acacaaattt aaaaagaaag 1380
aaaacagtct tccatagatt tttaaggaag aaagggccca agtcaggaga tcgcttggtt 1440
ttcttccaga agttaaatgg ggggatctga agatttgaat gttcggtctg ctttgaaatg 1500
tatgtctttt gggaatgtat tatatgccta gctttataat caggtataaa attttaatta 1560
ttcccaggaa tatgcataat attgaatatt tcatgtccta ttttaataga aaacctcagg 1620
gcccaagtaa ccagtgatag aagttagaaa aaccccttta cttagaattg tccacctagt 1680
cagagcccaa gaaagaattt tcagtggaaa aatcaatata taacttagtg ctagctagcg 1740
ccacagactc tagtagataa tattatcatc ataatggctg gtgaaaccat ataatcacag 1800
aaaaacattg ccttcagcat gttcagttcg cagcactgag ggcactcttg agggtgttgt 1860
taatgaagat ttaattttta aatacaggtg gttccaagct ttcaaatagg ttatgctcca 1920
aaagtgttat ttgtaagtta atttttttac aagtcaaaca atgttggaag tggtatttag 1980
gttctagatc ggtccacgaa agttagccca tatgtatatc ttgaatagta taggggaggg 2040
tattcataaa gtccttatgt ggttttaact aagtgaaatt atggacaaga gaaataattg 2100
taaaatcgtc ttaaaggcaa atttaatttt tacccctgtt tatgggacat tcgttctatt 2160
aactgtcaga cacaatttct gttttcatct gagagccagg tttcctttat ttctacatct 2220
aaaataagaa catattgtac actattatat aatacagaat tgtcttacac tttaataaat 2280
tcgcatttta aaggtgttta caggattatt ttttatatct gtagctgaat ttgttaaagt 2340
ctaaaaagct caaggacttt atgaagatct cattatatga ggaaaatcat aggttaccat 2400
tttataactc tattgccata agaaaataca ctctaaaatc ttgatttgaa acatattaga 2460
aaccttgatt cagtgctcag tggtctccta gtaagaagtc accgacggta gcgtcatatg 2520
agaagaaaga aatccccacc acctcaacct ctgctgagat tgtgtgctag gaacagcctt 2580
ccctccgttt cccctcagtc aaacttgagc cagcctctgg atcgatgtga tcttattgca 2640
tgtttccatg gggtgtacct atactttaag ccaatcctgc tgcattcact gctaagttaa 2700
ataaaaagcc aagaagaaaa aaaaaatttt gcactgtgca gatcctttgc tatctgactt 2760
gcatctcttc ccccacctgt cagctagcca cctgcttgtt tgtgttggga tattttttag 2820
cacctgaagc accatctgaa aggggcacca t 2851
<210>23
<211>3473
<212>DNA
<213>人
<400>23
aagagcagcg gcgaggcggc ggtggtggct gagtccgtgg tggcagaggc gaaggcgaca 60
gctctagggg ttggcaccgg ccccgagagg aggatgcggg tccggatagg gctgacgctg 120
ctgctgtgtg cggtgctgct gagcttggcc tcggcgtcct cggatgaaga aggcagccag 180
gatgaatcct tagattccaa gactactttg acatcagatg agtcagtaaa ggaccacact 240
actgcaggca gagtagttgc tggtcaaata tttcttgatt cagaagaatc tgaattagaa 300
tcctctattc aagaagagga agacagcctc aagagccaag agggggagag tgtcacagaa 360
gatatcagct ttctagagtc tccaaatcca gaaaacaagg actatgaaga gccaaagaaa 420
gtacggaaac cagctttgac cgccattgaa ggcacagcac atggggagcc ctgccacttc 480
ccttttcttt tcctagataa ggagtatgat gaatgtacat cagatgggag ggaagatggc 540
agactgtggt gtgctacaac ctatgactac aaagcagatg aaaagtgggg cttttgtgaa 600
actgaagaag aggctgctaa gagacggcag atgcaggaag cagaaatggt gtatcaaact 660
ggaatgaaaa tccttaatgg aagcaataag aaaagccaaa aaagagaagc atatcggtat 720
ctccaaaagg cagcaagcat gaaccatacc aaagccctgg agagagtgtc atatgctctt 780
ttatttggtg attacttgcc acagaatatc caggcagcga gagagatgtt tgagaagctg 840
actgaggaag gctctcccaa gggacagact gctcttggct ttctgtatgc ctctggactt 900
ggtgttaatt caagtcaggc aaaggctctt gtatattata catttggagc tcttgggggc 960
aatctaatag cccacatggt tttgggttac agatactggg ctggcatcgg cgtcctccag 1020
agttgtgaat ctgccctgac tcactatcgt cttgttgcca atcatgttgc tagtgatatc 1080
tcgctaacag gaggctcagt agtacagaga atacggctgc ctgatgaagt ggaaaatcca 1140
ggaatgaaca gtggaatgct agaagaagat ttgattcaat attaccagtt cctagctgaa 1200
aaaggtgatg tacaagcaca ggttggtctt ggacaactgc acctgcacgg agggcgtgga 1260
gtagaacaga atcatcagag agcatttgac tacttcaatt tagcagcaaa tgctggcaat 1320
tcacatgcca tggccttttt gggaaagatg tattcggaag gaagtgacat tgtacctcag 1380
agtaatgaga cagctctcca ctactttaag aaagctgctg acatgggcaa cccagttgga 1440
cagagtgggc ttggaatggc ctacctctat gggagaggag ttcaagttaa ttatgatcta 1500
gcccttaagt atttccagaa agctgctgaa caaggctggg tggatgggca gctacagctt 1560
ggttccatgt actataatgg cattggagtc aagagagatt ataaacaggc cttgaagtat 1620
tttaatttag cttctcaggg aggccatatc ttggctttct ataacctagc tcagatgcat 1680
gccagtggca ccggcgtgat gcgatcatgt cacactgcag tggagttgtt taagaatgta 1740
tgtgaacgag gccgttggtc tgaaaggctt atgactgcct ataacagcta taaagatggc 1800
gattacaatg ctgcagtgat ccagtacctc ctcctggctg aacagggcta tgaagtggca 1860
caaagcaatg cagcctttat tcttgatcag agagaagcaa gcattgtagg tgagaatgaa 1920
acttatccca gagctttgct acattggaac agggccgcct ctcaaggcta tactgtggct 1980
agaattaagc tcggagacta ccatttctat gggtttggca ccgatgtaga ttatgaaact 2040
gcatttattc attaccgtct ggcttctgag cagcaacaca gtgcacaagc tatgtttaat 2100
ctgggatata tgcatgagaa aggactgggc attaaacagg atattcacct tgcgaaacgt 2160
ttttatgaca tggcagctga agccagccca gatgcacaag ttccagtctt cctagccctc 2220
tgcaaattgg gcgtcgtcta tttcttgcag tacatacggg aaacaaacat tcgagatatg 2280
ttcacccaac ttgatatgga ccagcttttg ggacctgagt gggaccttta cctcatgacc 2340
atcattgcgc tgctgttggg aacagtcata gcttacaggc aaaggcagca ccaagacatg 2400
cctgcaccca ggcctccagg gccacggcca gctccacccc agcaggaggg gccaccagag 2460
cagcagccac cacagtaata ggcactgggt ccagccttga tcagtgacag cgaaggaagt 2520
tatctgctgg gaacacttgc atttgattta ggaccttgga tcagtggtca cctcccagaa 2580
gaggcacggc acaaggaagc attgaattcc taaagctgct tagaatctga tgcctttatt 2640
ttcagggata agtaactctt acctaaactg agctgaatgt ttgtttcagt gccatatgga 2700
ataacaactt tcagtggctt ttttttttct tttctggaaa catatgtgag acactcagag 2760
taatgtctac tgtatccagc tatctttctt ggatcctttt ggtcattatt tcagtgtgca 2820
taagttctta atgtcaacca tctttaaggt attgtgcatc gacactaaaa actgatcagt 2880
gtaaaaagga aaacccagtt gcaagtttaa acgtgttcga aagtctgaaa atagaacttg 2940
ccttttaagt taaaaaaaaa aaaagctatc ttgaaaatgt tttggaactg cgataactga 3000
gaaactctta ccagtccaca tgcaattaga catattcagc atatttgtta ttttaaaagg 3060
gagggttggg aggtttctta ttggtgattg tcacacggta taccatactc ctctccttca 3120
aagaatgaaa ggccttgtta aggagttttt tgtgagcttt acttctttgg aatggaatat 3180
acttatgcaa aaccttgtga actgactcct tgcactaacg cgagtttgcc ccacctactc 3240
tgtaatttgc ttgtttgttt tgaatataca gagccttgat ccagaagcca gaggatggac 3300
taagtgggag aaattagaaa acaaaacgaa ctctggttgg ggtactacga tcacagacac 3360
agacatactt ttcctaaagt tgaagcattt gttcccagga tttattttac tttgcatttc 3420
cttttgcaca aagaacacat caccatttcc ttttgcacaa agaacacatc acc 3473
<210>24
<211>401
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(252)..(252)
<223>各种碱基
<220>
<221>misc_feature
<222>(303)..(303)
<223>各种碱基
<220>
<221>misc feature
<222>(390)..(390)
<223>各种碱基
ttagattatt ttcaatttat tattcagaat aaatatatct tttttcttta acttctcaaa 60
tagttattga attgtattgg tttaaattaa atgcgtcatg tgtatatatc agtattaatt 120
caagagatac aaaaggaaat tgagtgaaaa ataagtctgc ctccttccca tcactctcat 180
gtctctacct agaggcaatt attgtcaaca gtttttgatg tgtctttcaa aaaatagtcc 240
attaagcctg gngtactaga tctcttttaa aagtttacaa cctgttacag aatatatata 300
aangttcaat tactagtaac accttattac agatacagat tacaacttag gaaatatatt 360
ttcatggacc attgatgtca tttggattcn cccctacaat c 401
<210>25
<211>1820
<212>DNA
<213>人
<400>25
aatgtcttag aaaaaggctt tctaaaagaa aaagagcaag aggccatttc ttttcaagat 60
agatacaaag aacttcagga aaaacataaa caagaattgg aagacatgag gaaagctggt 120
cacgaagccc tcagcattat tgtggatgaa tataaggcac tactgcagtc ttcagttaag 180
caacaagtag aagctattga aaaacagtac atttctgcaa ttgagaaaca ggcacacaag 240
tgtgaggagt tgctaaatgc tcagcatcag aggctccttg aaatgctaga tacagagaag 300
gaactgttaa aagaaaaaat aaaggaagct ttgattcagc aatctcaaga acagaaggaa 360
atattggaaa agtgtttgga ggaagaaagg caaagaaata aagaggcatt agtatccgct 420
gcaaagcttg aaaaagaagc agtgaaggat gcagttttaa aagtcgtaga agaagaaaga 480
aaaaatttag aaaaagcgca tgctgaagaa agggaattat ggaagacaga acatgcaaaa 540
gatcaagaaa aagtatctca ggaaattcaa aaagctatac aagaacaaag aaaaataagt 600
caggaaactg ttaaggcagc aataatagaa gagcagaaac gaagtgaaaa ggctgtggaa 660
gaggcagtga aaagaacaag agatgaattg atagagtata taaaagaaca gaaaaggctc 720
gatcaagtca tccgccaaag aagcctgtcc agtttggaac tgttcctctc ctgtgcacag 780
aaacagttaa gtgctttaat agctacggaa ccagttgaca ttgaataaaa agaacatgac 840
aaacccacac tggcattgga taaatcatat tacaccttca aaatacacac tctgaattat 900
aaagatgtgt ttgttttctt tccaaatcat gtagaattga tttccagttc aaggataaac 960
caaaacaata tttagaacta tcaagtgatc taatttattt tcttttggtt tcttctttac 1020
atttactgtt attttattat tattagtagt agcagcaaca gagtatgata tgacccaaaa 1080
gccattgtaa agtgccacat taccaaaatt aattaagtaa actttatagc ctgtgggagt 1140
ctattatata ttattttgca aaagtagtaa atatattatt gtttcatgat gactcttgat 1200
gagatgctag aatgtaacca tacatttatc ttattttgag gatagaaata gcatggattt 1260
caacatcact tatttatctg tataattgga aataaaacac cgatatgata gagaatcatt 1320
ccggcattac ctaacctctt ctgcagttgg atctatgtat tttcattggt ctactgaaaa 1380
cgaacaatac aattaaaagc actaaagatt attatattaa ttcaactttg atctgatata 1440
tcacttaaac taaaggggtg tgtgtggtgt atgcttgttt cctatttctg ctctttaaag 1500
atactttgaa tcaataaaac cattagtcta caaatcaaat tgtgaactta atctctagaa 1560
agagaatata actcagccat ttataggaat ttaggttcaa gtacaggata tatgaaatct 1620
tttcccagta tttcagaatg tacttaattc acaggcagga tgcttcaatg caaaatcatg 1680
aatattttta attcaaaact aaaatgtcat taatatgtat gtatgcaaat gttttatctt 1740
attttctgaa atgcatctac tttcatgggc tttgtacgtt tctgagattt ctcagtgtaa 1800
taaaaagagc tcccaaactt 1820
<210>26
<211>280
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(237)..(237)
<223>any kind of base
<220>
<221>misc_feature
<222>(261)..(261)
<223>any kind of base
<400>26
tcaagtcata agataaagtt taatcatttg atcatgttaa aagacacaaa acacagccaa 60
tctaaccaaa tttcaggcat gcatttacat aaatatatta aattaagaaa agaaattgta 120
cacttaaacg tccttttcac ctagaaatca ttaaatccac agatcaacaa taaaaccaat 180
tctctgcatt taccacttca agatacaatt gttctatttt aaagataaca caaactncac 240
tagtctggtt aggaatttat ntgcattata catatattat 280
<210>27
<211>392
<212>DNA
<213>人
<400>27
ttggtttgaa atggcacccc aggactttgg gcctgcctta cttgatagcc tcgttcagtg 60
agcaaagact tagtgagcag ctcttgtatg ccaagtattt tgctaagctc tggaaaaaag 120
ataaacaaga catggttctt gctttcaagg agtgtgtaat tctttagcca gatatggaaa 180
cctggaccct gagtgggaga aaggagacag atgaaaggag tccgtgattt tgtaaccaag 240
agctgcctgc atggttatga gtatcactga ttttagggac gcccacagag ctaaagcatt 300
tttttaatcc gagaagactt ttgtaactca tattagttaa tcttctagct ctgagatagc 360
aacacagctc ttagaattct gtaagtaagc tt 392
<210>28
<211>2299
<212>DNA
<213>人
<400>28
cgaaccccca cagctggagg gcgaggccag ctgtacccgg ccccagtgcc ctttcgcggc 60
cacaagcggc cgtcctcctg gtccggtgct ccggcgcctg atctaggttc atggagccgg 120
ggctgtggct ccttttcggg ctcacagtga cctccgccgc aggattcgtg ccttgctccc 180
agtctgggga tgctggcagg cgcggcgtgt cccaggcccc cactgcagcc agatctgagg 240
gggactgtga agagactgtg gctggccctg gcgaggagac tgtggctggc cctggcgagg 300
ggactgtggc cccgacagca ctgcagggtc caagccctgg aagccctggg caggagcagg 360
cggccgaggg ggcccctgag caccaccgat ccaggcgctg cacgtgcttc acctacaagg 420
acaaggagtg tgtctactat tgccacctgg acatcatttg gatcaacact cccgaacaga 480
cggtgcccta tggactgtcc aactacagag gaagcttccg gggcaagagg tctgcggggc 540
cacttccagg gaatctgcag ctctcacatc ggccacactt gcgctgcgct tgtgtgggga 600
gatatgacaa ggcctgcctg cacttttgca cccaaactct ggacgtcagc agacaggttg 660
aagtcaagga ccaacaaagc aagcaggctt tagacctcca ccatccaaag ctcatgcccg 720
gcagtggact cgccctcgct ccatctacct gcccccgctg cctctttcag gaaggagccc 780
cttaggagga caggcctgca gcatcctggt ctcgggaggc ttctgtcatt gctcacacac 840
agttcagatt tccacctctt tatagacaag aagtgaattt gcctggggca gaacacccac 900
ccaaagagtc cccacttaac aatacccccc ccccacggca agaatgccca aatccgaatg 960
accccagttt tcctaatgag taaaatgatc ccagatgtgc cccagagcat gacgcctgca 1020
gctccggttt catgcaggaa attggttttg gagagttttg gcaagttgga aagccactta 1080
ctggcttttg acatgacttc tcttggagaa taagtggact ccaagctaac tctttgcaaa 1140
tgtaaacaca tgtccatctt gtaataaatg caaaatgccc gtgcagcaga agcatgcgac 1200
tttcatatcc ttgcctagaa taggctgcat ggtgtatgtc agtgagggcc acgaggcgtc 1260
ggctttagac acagatcata gctctacagg agtttatgaa tttgaagctt atgggatttt 1320
ggcagagaaa ttttcagctg tgcttgatac ccaccaaaag aatgtatctc gaaagaatga 1380
aggaagaaga aaaaaggatc cttgatgttt gtgacaagaa aatgagaaag ttagtatctg 1440
caatacagag cttgttcctg ttcagtgact gaccctctgt attctgtata gacaccaggc 1500
cgatacacag tggagttccc aggccttgtt tgcaggaagc cgactgtaaa gacagcccca 1560
gctcaaggct attaggttga atatttgctt tcatgagtaa atgtggatct ttggggaatg 1620
gcttcaaaat aagtcacgaa cacaaattct ttgtaaatta tgtaaattcc tgtttatata 1680
aattggcaac aacttatacc gtctgacagt tcaaaatctc tttcagctgc gctcttccca 1740
ccgagccgag cttactgtga gtgtggagat gttatcccac catgtaaagt cgcctgcgca 1800
ggggagggct gcccatctcc ccaacccagt cacagagaga taggaaacgg catttgagtg 1860
ggtgtccagg gccccgtaga gagacattta agatggtgta tgacagagca ttggccttga 1920
ccaaatgtta aatcctctgt gtgtatttca taagttatta caggtataaa agtgatgacc 1980
tatcatgagg aaatgaaagt ggctgatttg ctggtaggat tttgtacagt ttagagaagc 2040
gattatttat tgtgaaactg ttctccactc caactccttt atgtggatct gttcaaagta 2100
gtcactgtat atacgtatag agaggtagat aggtaggtag attttaaatt gcattctgaa 2160
tacaaactca tactccttag agcttgaatt acatttttaa aatgcatatg tgctgtttgg 2220
caccgtggca agatggtatc agagagaaac ccatcaattg ctcaaatact cagaaagtac 2280
tgtcaaaagc ctaataaaa 2299
<210>29
<211>1339
<212>DNA
<213>人
<400>29
ctaaacaaaa tcattcactt ccctgatttt gataagaaaa ttcctgtaaa gctgtttcct 60
ctgcctctcc tctacgttgg aaaccacata agtggattat caagcacaag taaattaagc 120
ctaccgatgt tcaccgtgct caggaaattc accattccac ttaccttact tctggaaacc 180
atcatacttg ggaagcagta ttcactcaac atcatcctca gtgtctttgc cattattctc 240
ggggctttca tagcagctgg gtctgacctt gcttttaact tagaaggcta tatttttgta 300
ttcctgaatg atatcttcac atcagcaaat ggagtttata ccaaacagaa aatggaccca 360
aaggagctag ggaaatacgg agtacttttc tacaatgcct gcttcatgat tatcccaact 420
cttattatta gtgtctccac tggagacctc caacaggcta ctgaattcaa ccaatggaag 480
aatgttgtgt ttatcctaca gtttcttctt tcctgttttt tggggtttct gctgatgtac 540
tccacggttc tgtgcagcta ttacaattca gccctgacga cagcagtggt tggagccatc 600
aagaatgtat ccgttgccta cattgggata ttaatcggtg gagactacat tttctctttg 660
ttaaactttg tagggttaaa tatttgcatg gcagggggct tgagatattc ctttttaaca 720
ctgagcagcc agttaaaacc taaacctgtg ggtgaagaaa acatctgttt ggatttgaag 780
agctaaagag tctgcagcag gattggagac tgacttgtga ctgcgggctg ggggggcatt 840
cccagtagga atgtgaagcc agaggtttcg gattcgtgac atccaccccc tgggcaagtg 900
agagcatctg caaatgcaa agagaactac ctcatatgca ggatgagcca atggcagtct 960
caagaaatgt actcgggcga caccttacct gtggaaagca aatcttttca aaataagcca 1020
ctgggactcg gtaggtggag ccccagctgc tcttctaggg acctatgggg ccttcgtggc 1080
atctctgtgc tgtgtgctgg ggaggaggtt gatgtaatgg tgactctttt ctgatcagca 1140
ccttggccgt gattcccaag gtcccagcca aagcaaaggg ccagttgttt cagtttaaac 1200
agacatgtct ttagtctaat aaaattagtt aactgccagt aaagttattt gttagctttg 1260
atgaaagcta tgttggtatc tttccctaat catcaaagta aataaaaaat catttctatg 1320
taaaaaaaaa aaaaaaaaa 1339
<210>30
<211>4250
<212>DNA
<213>人
<400>30
gaacacatcg cgtttgcatc ccagaaagta gtcgccgcga ctatttcccc caaagagaca 60
agcacacatg taggaatgac aaaggcttgc gaaggagaga gcgcagcccg cggcccggag 120
agatcccctc gataatggat tactaaatgg gatacacgct gtaccagttc gctccgagcc 180
ccggccgcct gtccgtcgat gcaccgaaaa gggtgaagta gagaaataaa gtctccccgc 240
tgaactacta tgaggtcaga agccttgctg ctatatttca cactgctaca ctttgctggg 300
gctggtttcc cagaagattc tgagccaatc agtatttcgc atggcaacta tacaaaacag 360
tatccggtgt ttgtgggcca caagccagga cggaacacca cacagaggca caggctggac 420
atccagatga ttatgatcat gaacggaacc ctctacattg ctgctaggga ccatatttat 480
actgttgata tagacacatc acacacggaa gaaatttatt gtagcaaaaa actgacatgg 540
aaatctagac aggccgatgt agacacatgc agaatgaagg gaaaacataa ggatgagtgc 600
cacaacttta ttaaagttct tctaaagaaa aacgatgatg cattgtttgt ctgtggaact 660
aatgccttca acccttcctg cagaaactat aagatggata cattggaacc attcggggat 720
gaattcagcg gaatggccag atgcccatat gatgccaaac atgccaacgt tgcactgttt 780
gcagatggaa aactatactc agccacagtg actgacttcc ttgccattga cgcagtcatt 840
taccggagtc ttggagaaag ccctaccctg cggaccgtca agcacgattc aaaatggttg 900
aaagaaccat actttgttca agccgtggat tacggagatt atatctactt cttcttcagg 960
gaaatagcag tggagtataa caccatggga aaggtagttt tcccaagagt ggctcaggtt 1020
tgtaagaatg atatgggagg atctcaaaga gtcctggaga aacagtggac gtcgttcctg 1080
aaggcgcgct tgaactgctc agttcctgga gactctcatt tttatttcaa cattctccag 1140
gcagttacag atgtgattcg tatcaacggg cgtgatgttg tcctggcaac gttttctaca 1200
ccttataaca gcatccctgg gtctgcagtc tgtgcctatg acatgcttga cattgccagt 1260
gtttttactg ggagattcaa ggaacagaag tctcctgatt ccacctggac accagttcct 1320
gatgaacgag ttcctaagcc caggccaggt tgctgtgctg gctcatcctc cttagaaaga 1380
tatgcaacct ccaatgagtt ccctgatgat accctgaact tcatcaagac gcacccgctc 1440
atggatgagg cagtgccctc catcttcaac aggccatggt tcctgagaac aatggtcaga 1500
taccgcctta ccaaaattgc agtggacaca gctgctgggc catatcagaa tcacactgtg 1560
gtttttctgg gatcagagaa gggaatcatc ttgaagtttt tggccagaat aggaaatagt 1620
ggttttctaa atgacagcct tttcctggag gagatgagtg tttacaactc tgaaaaatgc 1680
agctatgatg gagtcgaaga caaaaggatc atgggcatgc agctggacag agcaagcagc 1740
tctctgtatg ttgcgttctc tacctgtgtg ataaaggttc cccttggccg gtgtgaacga 1800
catgggaagt gtaaaaaaac ctgtattgcc tccagagacc catattgtgg atggataaag 1860
gaaggtggtg cctgcagcca tttatcaccc aacagcagac tgacttttga gcaggacata 1920
gagcgtggca atacagatgg tctgggggac tgtcacaatt cctttgtggc actgaatgac 1980
atttcaactc ctctaccaga taatgaaatg tcttacaaca cagtgtatgg gcattccagt 2040
tccctcttgc ccagcacaac cacatcagat tcgacggctc aagaggggta tgagtctagg 2100
ggaggaatgc tggactggaa gcatctgctt gactcacctg acagcacaga ccctttgggg 2160
gcagtgtctt cccataatca ccaagacaag aagggagtga ttcgggaaag ttacctcaaa 2220
ggccacgacc agctggttcc cgtcaccctc ttggccattg cagtcatcct ggctttcgtc 2280
atgggggccg tcttctcggg catcaccgtc tactgcgtct gtgatcatcg gcgcaaagac 2340
gtggctgtgg tgcagcgcaa ggagaaggag ctcacccact cgcgccgggg ctccatgagc 2400
agcgtcacca agctcagcgg cctctttggg gacactcaat ccaaagaccc aaagccggag 2460
gccatcctca cgccactcat gcacaacggc aagctcgcca ctcccggcaa cacggccaag 2520
atgctcatta aagcagacca gcaccacctg gacctgacgg ccctccccac cccagagtca 2580
accccaacgc tgcagcagaa gcggaagccc agccgcggca gccgcgagtg ggagaggaac 2640
cagaacctca tcaatgcctg cacaaaggac atgcccccca tgggctcccc tgtgattccc 2700
acggacctgc ccctgcgggc ctcccccagc cacatcccca gcgtggtggt cctgcccatc 2760
acgcagcagg gctaccagca tgagtacgtg gaccagccca aaatgagcga ggtggcccag 2820
atggcgctgg aggaccaggc cgccacactg gagtataaga ccatcaagga acatctcagc 2880
agcaagagtc ccaaccatgg ggtgaacctt gtggagaacc tggacagcct gccccccaaa 2940
gttccacagc gggaggcctc cctgggtccc ccgggagcct ccctgtctca gaccggtcta 3000
agcaagcggc tggaaatgca ccactcctct tcctacgggg ttgactataa gaggagctac 3060
cccacgaact cgctcacgag aagccaccag gccaccactc tcaaaagaaa caacactaac 3120
tcctccaatt cctctcacct ctccagaaac cagagctttg gcaggggaga caacccgccg 3180
cccgccccgc agagggtgga ctccatccag gtgcacagct cccagccatc tggccaggcc 3240
gtgactgtct cgaggcagcc cagcctcaac gcctacaact cactgacaag gtcggggctg 3300
aagcgtacgc cctcgctaaa gccggacgta ccccccaaac catcctttgc tcccctttcc 3360
acatccatga agcccaatga tgcgtgtaca taatcccagg gggagggggt caggtgtcga 3420
accagcaggc aaggcgaggt gcccgctcag ctcagcaagg ttctcaactg cctcgagtac 3480
ccaccagacc aagaaggcct gcggcagagc cgaggacgct gggtcctcct ctctgggaca 3540
caggggtact cacgaaaact gggccgcgtg gtttggtgaa ggtttgcaac ggcggggact 3600
caccttcatt ctcttccttc actttccccc acaccctaca acaggtcgga cccacaaaag 3660
acttcagtta tcatcacaaa catgagccaa aagcacatac ctaccccatc ccccaccccc 3720
acacacacac acacatgcac acaacacata cacacacacg cacagaggtg aacagaaact 3780
gaaacatttt gtccacaact tcacgggacg tggccagact gggtttgcgt tccaacctgc 3840
aaaacacaaa tacatttttt aaaatcaaga aaatttaaaa agacaaaaaa aaaagaattc 3900
attgataatt ctaactcaga ctttaacaat ggcagaagtt tactatgcgc aaatactgtg 3960
aaatgcccgc cagtgttaca gctttctgtt gcagcagata aatgccatgt tgggcaacta 4020
tgtcatagat ttctgctcct cctctctttt aatgaaataa cgtgaccgtt aacgcaagta 4080
actctttatt tattgttcac cctttttttc cttaaggaaa ggactcttcc aaatatcatc 4140
ctatgaacag ctcttcagaa agcccattga aagttaaact atttaacgtg aaatccatta 4200
actggaataa ttgagtttct ttatttttac aataaattca ctgagtaaat 4250
<210>31
<211>2785
<212>DNA
<213>人
<400>31
ctttagccca acagtcaaaa ataattgatg ctaccctaca aatgtccaaa actctagtat 60
atcatatttc taagttacag caaatattag tcctgctaaa ccagggagct ttggcaaaaa 120
tgttttttga cagtaaattt gtccttgatt atatattaac tagtcaaaga ggtgtttgta 180
acattattag agcttcttgt tgtaggtggg ttaacaccac caatcaagag gtcattctaa 240
cagaaagcct ggatcagaaa accatcaccc taaaaaaaca tgccttacat atttaacaca 300
ctctgaaatc cagtcaaaat atgactaaag gcccttgcca tgactgatgt attctcctgg 360
ccaacgccaa acaaatggga gcctggttac gagtcagcct tcagggactt gtcacatttc 420
tacttggttt cttccttgtt attgtcataa taaaatgttt tctatgctgt ttagtgcaac 480
ttaggcccta ttctgtagaa gtctcctcta ctattcaggc cactcaaaca ccccaaataa 540
ttgagttcaa aatcgacatc aagatataaa ggaatcagtg actaaatata tttcatatat 600
ggtattttta ttgattattg tgctgtcttg acctagtatg gaggccttgg ctagaggctg 660
gtcagtttcc tctcttgagc agctgattaa atccacaccc caaccacttc ccttatcagg 720
ttctcacact ctggggccac tatgtaccca ctctaatcac cacagggcca gacatcagac 780
aattaaggac agcgcccatg ccccaaagcc cgccaaaatt atgcaaatta ttcaaaatta 840
ttcaacctag ctaaccccac cctttttgct gtacataagc tgcccattcc ccctccagcc 900
tgtggtaccc agtcctcagg tgcaaccccc tgcgtggtcc tctgtggcag ccttctctca 960
ttcagagctg ttttccacag aggtagtgaa aagaactgga ttttcaagtt cactttgcaa 1020
gagaaaaaga aaactcagta gaagataatg gcaagtccag actggggata tgatgacaaa 1080
aatggtcctg aacaatggag caagctgtat cccattgcca atggaaataa ccaatcccct 1140
gttgatatta aaaccagtga aaccaaacat gacacctctc tgaaacctat tagtgtctcc 1200
tacaacccag ccacagccaa agaaattatc aatgtggggc attctttcca tgtaaatttt 1260
gaggacaacg ataaccgatc agtgctgaaa ggtggtcctt tctctgacag ctacaggctc 1320
tttcagtttc attttcactg gggcagtaca aatgagcatg gttcagaaca tacagtggat 1380
ggagtcaaat attctgccga gcttcacgta gctcactgga attctgcaaa gtactccagc 1440
cttgctgaag ctgcctcaaa ggctgatggt ttggcagtta ttggtgtttt gatgaaggtt 1500
ggtgaggcca acccaaagct gcagaaagta cttgatgccc tccaagcaat taaaaccaag 1560
ggcaaacgag ccccattcac aaattttgac ccctctactc tccttccttc atccctggat 1620
ttctggacct accctggctc tctgactcat cctcctcttt atgagagtgt aacttggatc 1680
atctgtaagg agagcatcag tgtcagctca gagcagctgg cacaattccg cagccttcta 1740
tcaaatgttg aaggtgataa cgctgtcccc atgcagcaca acaaccgccc aacccaacct 1800
ctgaagggca gaacagtgag agcttcattt tgatgattct gagaagaaac ttgtccttcc 1860
tcaagaacac agccctgctt ctgacataat ccagttaaaa taataatttt taagaaataa 1920
atttatttca atattagcaa gacagcatgc cttcaaatca atctgtaaaa ctaagaaact 1980
taaattttag ttcttactgc ttaattcaaa taataattag taagctagca aatagtaatc 2040
tgtaagcata agcttatctt aaattcaagt ttagtttgag gaattcttta aaattacaac 2100
taagtgattt gtatgtctat ttttttcagt ttatttgaac caataaaata attttatctc 2160
tttctttctg ttgtgcattc agtttctaaa accattaagt ttctactcca tttacattca 2220
aaaatcttaa atactttact tgcaagagta ttttgcttca aatacaacaa cctaagagca 2280
gctggagatg aaatattggg aaattcattt gcttactcct gaagacaaaa atatagctga 2340
gatgaccact ggatttaata tcgttatgct ggcccaacat tgctaccatt tgtgttgtct 2400
gtgatcaaaa tgattatctt ttatatagga agatgacgct tctggatatt gctttcactt 2460
cttctcccca cgttagcaag gacaatgctt ctctgccatt attacaacta gttagtttgc 2520
atggagaatc tttactttaa aattggaaga aaagtcacaa gtgaatggtt tataaaaatg 2580
ctaaagaagt cattcttgct tagaatcata tagaaacatc atgcaatctt ttagtcagat 2640
gtgcgcttca ccttatgcta tttttatctt taattgacac acaataattg tacatgttta 2700
tggagtatag tgtggtgttt tctgtttgtt tgtttgtttt ttgagacaag gtctcactct 2760
gccagtcagg gtggagtgcg atggt 2785
<210>32
<211>9588
<212>DNA
<213>人
<400>32
ccgaccaaca ccaacaccca gctccgacgc agctcctctg cgcccttgcc gccctccgag 60
ccacagcttt cctcccgctc ctgcccccgg cccgtcgccg tctccgcgct cgcagcggcc 120
tcgggagggc ccaggtagcg agcagcgacc tcgcgagcct tccgcactcc cgcccggttc 180
cccggccgtc cgcctatcct tggccccctc cgctttctcc gcgccggccc gcctcgctta 240
tgcctcggcg ctgagccgct ctcccgattg cccgccgaca tgagctgcaa cggaggctcc 300
cacccgcgga tcaacactct gggccgcatg atccgcgccg agtctggccc ggacctgcgc 360
tacgaggtga ccagcggcgg cgggggcacc agcaggatgt actattctcg gcgcggcgtg 420
atcaccgacc agaactcgga cggctactgt caaaccggca cgatgtccag gcaccagaac 480
cagaacacca tccaggagct gctgcagaac tgctccgact gcttgatgcg agcagagctc 540
atcgtgcagc ctgaattgaa gtatggagat ggaatacaac tgactcggag tcgagaattg 600
gatgagtgtt ttgcccaggc caatgaccaa atggaaatcc tcgacagctt gatcagagag 660
atgcggcaga tgggccagcc ctgtgatgct taccagaaaa ggcttcttca gctccaagag 720
caaatgcgag ccctttataa agccatcagt gtccctcgag tccgcagggc cagctccaag 780
ggtggtggag gctacacttg tcagagtggc tctggctggg atgagttcac caaacatgtc 840
accagtgaat gtttggggtg gatgaggcag caaagggcgg agatggacat ggtggcctgg 900
ggtgtggacc tggcctcagt ggagcagcac attaacagcc accggggcat ccacaactcc 960
atcggcgact atcgctggca gctggacaaa atcaaagccg acctgcgcga gaaatctgcg 1020
atctaccagt tggaggagga gtatgaaaac ctgctgaaag cgtcctttga gaggatggat 1080
cacctgcgac agctgcagaa catcattcag gccacgtcca gggagatcat gtggatcaat 1140
gactgcgagg aggaggagct gctgtacgac tggagcgaca agaacaccaa catcgctcag 1200
aaacaggagg ccttctccat acgcatgagt caactggaag ttaaagaaaa agagctcaat 1260
aagctgaaac aagaaagtga ccaacttgtc ctcaatcagc atccagcttc agacaaaatt 1320
gaggcctata tggacactct gcagacgcag tggagttgga ttcttcagat caccaagtgc 1380
attgatgttc atctgaaaga aaatgctgcc tactttcagt tttttgaaga ggcgcagtct 1440
actgaagcat acctgaaggg gctccaggac tccatcagga agaagtaccc ctgcgacaag 1500
aacatgcccc tgcagcacct gctggaacag atcaaggagc tggagaaaga acgagagaaa 1560
atccttgaat acaagcgtca ggtgcagaac ttggtaaaca agtctaagaa gattgtacag 1620
ctgaagcctc gtaacccaga ctacagaagc aataaaccca ttattctcag agctctctgt 1680
gactacaaac aagatcagaa aatcgtgcat aagggggatg agtgtatcct gaaggacaac 1740
aacgagcgca gcaagtggta cgtgacgggc ccgggaggcg ttgacatgct tgttccctct 1800
gtggggctga tcatccctcc tccgaaccca ctggccgtgg acctctcttg caagattgag 1860
cagtactacg aagccatctt ggctctgtgg aaccagctct acatcaacat gaagagcctg 1920
gtgtcctggc actactgcat gattgacata gagaagatca gggccatgac aatcgccaag 1980
ctgaaaacaa tgcggcagga agattacatg aagacgatag ccgaccttga gttacattac 2040
caagagttca tcagaaatag ccaaggctca gagatgtttg gagatgatga caagcggaaa 2100
atacagtctc agttcaccga tgcccagaag cattaccaga ccctggtcat tcagctccct 2160
ggctatcccc agcaccagac agtgaccaca actgaaatca ctcatcatgg aacctgccaa 2220
gatgtcaacc ataataaagt aattgaaacc aacagagaaa atgacaagca agaaacatgg 2280
atgctgatgg agctgcagaa gattcgcagg cagatagagc actgcgaggg caggatgact 2340
ctcaaaaacc tccctctagc agaccagggg tcttctcacc acatcacagt gaaaattaac 2400
gagcttaaga gtgtgcagaa tgattcacaa gcaattgctg aggttctcaa ccagcttaaa 2460
gatatgcttg ccaacttcag aggttctgaa aagtactgct atttacagaa tgaagtattt 2520
ggactatttc agaaactgga aaatatcaat ggtgttacag atggctactt aaatagctta 2580
tgcacagtaa gggcactgct ccaggctatt ctccaaacag aagacatgtt aaaggtttat 2640
gaagccaggc tcactgagga ggaaactgtc tgcctggacc tggataaagt ggaagcttac 2700
cgctgtggac tgaagaaaat aaaaaatgac ttgaacttga agaagtcgtt gttggccact 2760
atgaagacag aactacagaa agcccagcag atccactctc agacttcaca gcagtatcca 2820
ctttatgatc tggacttggg caagttcggt gaaaaagtca cacagctgac agaccgctgg 2880
caaaggatag ataaacagat cgactttaga ttatgggacc tggagaaaca aatcaagcaa 2940
ttgaggaatt atcgtgataa ctatcaggct ttctgcaagt ggctctatga tcgtaaacgc 3000
cgccaggatt ccttagaatc catgaaattt ggagattcca acacagtcat gcggtttttg 3060
aatgagcaga agaacttgca cagtgaaata tctggcaaac gagacaaatc agaggaagta 3120
caaaaaattg ctgaactttg cgccaattca attaaggatt atgagctcca gctggcctca 3180
tacacctcag gactggaaac tctgctgaac atacctatca agaggaccat gattcagtcc 3240
ccttctgggg tgattctgca agaggctgca gatgttcatg ctcggtacat tgaactactt 3300
acaagatctg gagactatta caggttctta agtgagatgc tgaagagttt ggaagatctg 3360
aagctgaaaa ataccaagat cgaagttttg gaagaggagc tcagactggc ccgagatgcc 3420
aactcggaaa actgtaataa gaacaaattc ctggatcaga acctgcagaa ataccaggca 3480
gagtgttccc agttcaaagc gaagcttgcg agcctggagg agctgaagag acaggctgag 3540
ctggatggga agtcggctaa gcaaaatcta gacaagtgct acggccaaat aaaagaactc 3600
aatgagaaga tcacccgact gacttatgag attgaagatg aaaagagaag aagaaaatct 3660
gtggaagaca gatttgacca acagaagaat gactatgacc aactgcagaa agcaaggcaa 3720
tgtgaaaagg agaaccttgg ttggcagaaa ttagagtctg agaaagccat caaggagaag 3780
gagtacgaga ttgaaaggtt gagggttcta ctgcaggaag aaggcacccg gaagagagaa 3840
tatgaaaatg agctggcaaa ggtaagaaac cactataatg aggagatgag taatttaagg 3900
aacaagtatg aaacagagat taacattacg aagaccacca tcaaggagat atccatgcaa 3960
aaagaggatg attccaaaaa tcttagaaac cagcttgata gactttcaag ggaaaatcga 4020
gatctgaagg atgaaattgt caggctcaat gacagcatct tgcaggccac tgagcagcga 4080
aggcgagctg aagaaaacgc ccttcagcaa aaggcctgtg gctctgagat aatgcagaag 4140
aagcagcatc tggagataga actgaagcag gtcatgcagc agcgctctga ggacaatgcc 4200
cggcacaagc agtccctgga ggaggctgcc aagaccattc aggacaaaaa taaggagatc 4260
gagagactca aagctgagtt tcaggaggag gccaagcgcc gctgggaata tgaaaatgaa 4320
ctgagtaagg taagaaacaa ttatgatgag gagatcatta gcttaaaaaa tcagtttgag 4380
accgagatca acatcaccaa gaccaccatc caccagctca ccatgcagaa ggaagaggat 4440
accagtggct accgggctca gatagacaat ctcacccgag aaaacaggag cttatctgaa 4500
gaaataaaga ggctgaagaa cactctaacc cagaccacag agaatctcag gagggtggaa 4560
gaagacatcc aacagcaaaa ggccactggc tctgaggtgt ctcagaggaa acagcagctg 4620
gaggttgagc tgagacaagt cactcagatg cgaacagagg agagcgtaag atataagcaa 4680
tctcttgatg atgctgccaa aaccatccag gataaaaaca aggagataga aaggttaaaa 4740
caactgatcg acaaagaaac aaatgaccgg aaatgcctgg aagatgaaaa cgcgagatta 4800
caaagggtcc agtatgacct gcagaaagca aacagtagtg cgacggagac aataaacaaa 4860
ctgaaggttc aggagcaaga actgacacgc ctgaggatcg actatgaaag ggtttcccag 4920
gagaggactg tgaaggacca ggatatcacg cggttccaga actctctgaa agagctgcag 4980
ctgcagaagc agaaggtgga agaggagctg aatcggctga agaggaccgc gtcagaagac 5040
tcctgcaaga ggaagaagct ggaggaagag ctggaaggca tgaggaggtc gctgaaggag 5100
caagccatca aaatcaccaa cctgacccag cagctggagc aggcatccat tgttaagaag 5160
aggagtgagg atgacctccg gcagcagagg gacgtgctgg atggccacct gagggaaaag 5220
cagaggaccc aggaagagct gaggaggctc tcttctgagg tcgaggccct gaggcggcag 5280
ttactccagg aacaggaaag tgtcaaacaa gctcacttga ggaatgagca tttccagaag 5340
gcgatagaag ataaaagcag aagcttaaat gaaagcaaaa tagaaattga gaggctgcag 5400
tctctcacag agaacctgac caaggagcac ttgatgttag aagaagaact gcggaacctg 5460
aggctggagt acgatgacct gaggagagga cgaagcgaag cggacagtga taaaaatgca 5520
accatcttgg aactaaggag ccagctgcag atcagcaaca accggaccct ggaactgcag 5580
gggctgatta atgatttaca gagagagagg gaaaatttga gacaggaaat tgagaaattc 5640
caaaagcagg ctttagaggc atctaatagg attcaggaat caaagaatca gtgtactcag 5700
gtggtacagg aaagagagag ccttctggtg aaaatcaaag tcctggagca agacaaggca 5760
aggctgcaga ggctggagga tgagctgaat cgtgcaaaat caactctaga ggcagaaacc 5820
agggtgaaac agcgcctgga gtgtgagaaa cagcaaattc agaatgacct gaatcagtgg 5880
aagactcaat attcccgcaa ggaggaggct attaggaaga tagaatcgga aagagaaaag 5940
agtgagagag agaagaacag tcttaggagt gagatcgaaa gactccaagc agagatcaag 6000
agaattgaag agaggtgcag gcgtaagctg gaggattcta ccagggagac acagtcacag 6060
ttagaaacag aacgctcccg atatcagagg gagattgata aactcagaca gcgcccatat 6120
gggtcccatc gagagaccca gactgagtgt gagtggaccg ttgacacctc caagctggtg 6180
tttgatgggc tgaggaagaa ggtgacagca atgcagctct atgagtgtca gctgatcgac 6240
aaaacaacct tggacaaact attgaagggg aagaagtcag tggaagaagt tgcttctgaa 6300
atccagccat tccttcgggg tgcaggatct atcgctggag catctgcttc tcctaaggaa 6360
aaatactctt tggtagaggc caagagaaag aaattaatca gcccagaatc cacagtcatg 6420
cttctggagg cccaggcagc tacaggtggt ataattgatc cccatcggaa tgagaagctg 6480
actgtcgaca gtgccatagc tcgggacctc attgacttcg atgaccgtca gcagatatat 6540
gcagcagaaa aagctatcac tggttttgat gatccatttt caggcaagac agtatctgtt 6600
tcagaagcca tcaagaaaaa tttgattgat agagaaaccg gaatgcgcct gctggaagcc 6660
cagattgctt cagggggtgt agtagaccct gtgaacagtg tctttttgcc aaaagatgtc 6720
gccttggccc gggggctgat tgatagagat ttgtatcgat ccctgaatga tccccgagat 6780
agtcagaaaa actttgtgga tccagtcacc aaaaagaagg tcagttacgt gcagctgaag 6840
gaacggtgca gaatcgaacc acatactggt ctgctcttgc tttcagtaca gaagagaagc 6900
atgtccttcc aaggaatcag acaacctgtg accgtcactg agctagtaga ttctggtata 6960
ttgagaccgt ccactgtcaa tgaactggaa tctggtcaga tttcttatga cgaggttggt 7020
gagagaatta aggacttcct ccagggttca agctgcatag caggcatata caatgagacc 7080
acaaaacaga agcttggcat ttatgaggcc atgaaaattg gcttagtccg acctggtact 7140
gctctggagt tgctggaagc ccaagcagct actggcttta tagtggatcc tgttagcaac 7200
ttgaggttac cagtggagga agcctacaag agaggtctgg tgggcattga gttcaaagag 7260
aagctcctgt ctgcagaacg agctgtcact gggtataatg atcctgaaac aggaaacatc 7320
atctctttgt tccaagccat gaataaggaa ctcatcgaaa agggccacgg tattcgctta 7380
ttagaagcac agatcgcaac cggggggatc attgacccaa aggagagcca tcgtttacca 7440
gttgacatag catataagag gggctatttc aatgaggaac tcagtgagat tctctcagat 7500
ccaagtgatg ataccaaagg attttttgac cccaacactg aagaaaatct tacctatctg 7560
caactaaaag aaagatgcat taaggatgag gaaacagggc tctgtcttct gcctctgaaa 7620
gaaaagaaga aacaggtgca gacatcacaa aagaataccc tcaggaagcg tagagtggtc 7680
atagttgacc cagaaaccaa taaagaaatg tctgttcagg aggcctacaa gaagggccta 7740
attgattatg aaaccttcaa agaactgtgt gagcaggaat gtgaatggga agaaataacc 7800
atcacgggat cagatggctc caccagggtg gtcctggtag atagaaagac aggcagtcag 7860
tatgatattc aagatgctat tgacaagggc cttgttgaca ggaagttctt tgatcagtac 7920
cgatccggca gcctcagcct cactcaattt gctgacatga tctccttgaa aaatggtgtc 7980
ggcaccagca gcagcatggg cagtggtgtc agcgatgatg tttttagcag ctcccgacat 8040
gaatcagtaa gtaagatttc caccatatcc agcgtcagga atttaaccat aaggagcagc 8100
tctttttcag acaccctgga agaatcgagc cccattgcag ccatctttga cacagaaaac 8160
ctggagaaaa tctccattac agaaggtata gagcggggca tcgttgacag catcacgggt 8220
cagaggcttc tggaggctca ggcctgcaca ggtggcatca tccacccaac cacgggccag 8280
aagctgtcac ttcaggacgc agtctcccag ggtgtgattg accaagacat ggccaccagc 8340
gtgaagcctg ctcagaaagc cttcataggc ttcgagggtg tgaagggaaa gaagaagatg 8400
tcagcagcag aggcagtgaa agaaaaatgg ctcccgtatg aggctggcca gcgcttcctg 8460
gagttccagt acctcacggg aggtcttgtt gacccggaag tgcatgggag gataagcacc 8520
gaagaagcca tccggaaggg gttcatagat ggccgcgccg cacagaggct gcaagacacc 8580
agcagctatg ccaaaatcct gacctgcccc aaaaccaaat taaaaatatc ctataaggat 8640
gccataaatc gctccatggt agaagatatc actgggctgc gccttctgga agccgcctcc 8700
gtgtcgtcca agggcttacc cagcccttac aacatgtctt cggctccggg gtcccgctcc 8760
ggctcccgct cgggatctcg ctccggatct cgctccgggt cccgcagtgg gtcccggaga 8820
ggaagctttg acgccacagg gaattcttcc tactcttatt cctactcatt tagcagtagt 8880
tctattgggc actagtagtc agttgggagt ggttgctata ccttgacttc atttatatga 8940
atttccactt tattaaataa tagaaaagaa aatcccggtg cttgcagtag agtgatagga 9000
cattctatgc ttacagaaaa tatagccatg attgaaatca aatagtaaag gctgttctgg 9060
ctttttatct tcttagctca tcttaaataa gcagtacact tggatgcagt gcgtctgaag 9120
tgctaatcag ttgtaacaat agcacaaatc gaacttagga tttgtttctt ctcttctgtg 9180
tttcgatttt tgatcaattc tttaattttg gaagcctata atacagtttt ctattcttgg 9240
agataaaaat taaatggatc actgatattt tagtcattct gcttctcatc taaatatttc 9300
catattctgt attaggagaa aattaccctc ccagcaccag cccccctctc aaacccccaa 9360
cccaaaacca agcattttgg aatgagtctc ctttagtttc agagtgtgga ttgtataacc 9420
catatactct tcgatgtact tgtttggttt ggtattaatt tgactgtgca tgacagcggc 9480
aatcttttct ttggtcaaag ttttctgttt attttgcttg tcatattcga tgtactttaa 9540
ggtgtcttta tgaagtttgc tattctggca ataaactttt agactttt 9588
<210>33
<211>366
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(351)..(351)
<223>各种碱基
<400>33
gaagtgccat ttatatttat acaaaaatat tacataattc agttagtatt ggtgacataa 60
tttagttagt atgggtgata taatggtcat aatttttagc atctaataaa gatcttttta 120
tgagtcccat ataaaatatg tgaacaaagc aatcttgtca taagatttgt gatgatttag 180
gagaaagtac tttgagataa tttttttctg tctctttgtg aactctctca acagtagttc 240
tctttagatt agagccagca ggtcggccat aacagttttc ttcaaatttg ggcaacagtt 300
atacaaatgc ttgaatttca agacaacata ttaaagggtc tatgaactgg naatctaacc 360
tgggtt 366
<210>34
<211>1466
<212>DNA
<213>人
<400>34
agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60
cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120
gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180
cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240
catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300
aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360
gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420
gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480
agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540
gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600
agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660
aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720
cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780
ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840
aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900
ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960
gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc 1020
ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag 1080
gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg 1140
agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag 1200
gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct 1260
ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca 1320
tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg 1380
ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca 1440
cagaaataaa gagctgttat actgtg 1466
<210>35
<211>187
<212>DNA
<213>人
<400>35
gatctggtgc attccggtcg acactctcgt ttatttggac tgtaagtctg acctctatga 60
ataattactt cagcccctga gtgctcccgg gccaagctcc ttggccaaac tttcacctta 120
gcttctgata agtcttgggc caagctaagc agcatctatc aatcatccct tcagctcctg 180
attgatc 187
<210>36
<211>2913
<212>DNA
<213>人
<400>36
actgggtacc gaggactggg tgtgtttaag gcagacagcc aggtgaggat cccagctact 60
ggggcctgct gtcatctcct gggagtaccc gggggtcagg agcctagggg actcttgcac 120
ttcacatcca gccatgctaa ttacactttt tggcaaagga aacagctagg agcagtttct 180
ttcactccta cagccccgtt ttctcagtgt ttagacctcg aattattact gggctagagg 240
gaaggcagcc tctgaagtgt ggcaggagga ggggaagtct gcctgcatct tggtgtgtct 300
gtcagatgcc agcactaata acctggcttc tgtgaggcct gtcagtgctc tcaggaatga 360
aaggggaccc ctgagaggtg ctcagtacca gcaggctgtg aatgctctct acccaccacc 420
ctcacctcct cgttaaagat ggtgctacct gccacacagc agacatctgg tcgctgcaca 480
cccgaaagac cccaaggcag tctgcccctt gtccagccac acgccagcac ccaccctcct 540
ggcccctgcc tcggcctccc cagaccagct gcacccagcc cccaacacgc accccttctc 600
cagatgtgtg cagggcctca ttttgcagag caaagacaga tgtttcagcc acacgcttta 660
ttaacttcta aaacctgtgc tcaggacact cttcaacagt catgaaaagt ttgatcactt 720
gccacagtca ggacctttgt gtggggctct gatctgatgt tcggtctcat catctcccaa 780
accagcagtc gtttgtaccc caaccctctg ctcaggggct cataccccca aatgattttc 840
ctgatttatg tatttcccta caaagggctt tctataccta gcatctgcct ccagcatgag 900
aagggggaat aggtgagacc catttgccag tagcagacgg ggaccctggg gagaaaatgg 960
cagagcctgt tggagactcc ctgtctccag ctgaccagcc aatgggattc ctcttccctc 1020
cactgtctcc cacaaagtag aagaatcctg gtacatttag cccatgagcc tggcacagat 1080
ccctatctag acatgaggcc ctttagacat gactttggca ttgaccagcc tgttggcaat 1140
gggtcgggga ggcagagggg atgctcacac cagtaattct catcccctga atgcttggga 1200
tcacctgggg agagttcaca aaatactggt gcaggggtcc cacctctgat gatgctgagt 1260
ggtgggtctg gggtgtggcc caggcatcat gatgtttcag gcccccaggt gacttcttag 1320
gcagcccagc taagccccta gagccttgca atttccccca aatgacctca gagggcccga 1380
tttgagggaa atgcctaact tcaggggccg taagaatccc ccagggagca tgtgaaatgc 1440
agataccagg cccaccccca gagatgagct gaggtgggtc aggggtgaag tgcagggatc 1500
agtgtttttc acaagctcca tacctccagg aaatggtgtt gtggttgggc ccgtagaaaa 1560
cattctgaga gtcctgttgc ctgtgccttg gtgcacgtgg ggtggaatcc cagtggccct 1620
gccttgagga ggatgtgcat taacgtggta ggggagacag agacagctcc acctgccccc 1680
tgtcccaccg gggacctcca aaaacttcat ggatgttaga gcaagcagcc atgctgcagc 1740
agaggatgag gctggcggat ttagtaagag ccctctgtgt ttgggctgag ttctttctct 1800
agttgccctg tcatctggcc tctggataac ccacctctcc tccctcatcc taaaattaca 1860
gatggcgaaa gacggccaca tttagtgaga cccctaaggt cctccaacta gggtgggtcc 1920
acagtggccc ctggtgcatg gaccacacac tctcttccct cctctggctc aggactacgg 1980
tctgaaatta gggagatatg aatgtctttc ttgaaaactt ctcttcccag tcttcccact 2040
ttgcttgggg gtccttggtc aaggccagct ttggactagg gcttgttgcg actaccagct 2100
gtctcatttt gctgtactgc aaactcaggc ttggttccaa gcttatgggg gccctgtcct 2160
tcccctagta gggtttgttt tggggtcaca tctggtcata cccttcagag agctcttccc 2220
cagcctctac atcagggaga gaggtaggta gggaggagca ttcaaggatt agaagaagga 2280
ctaaagtaca acagccttgg aggaactgcc aggaactaag ggcgagcact ggagaaggca 2340
acctgggacc ccctgcgctt ctgagcagga agaccaagac cttcaggggc cctaagcact 2400
gaaaacatca ttcctcatcc ccaagccctg gcatccccct gttcttctaa aataattctt 2460
ttctaggtat ttctgattgc aaaattctgg atgggttcat ccaagctgac ctttgctgtt 2520
ttttcccttc ccaacaaggc ctcacttttt ggagccacct tagttggtgc ctaggcagag 2580
gggcagtcag cagtggttat caggatcctg gctctatggg ttgccttcct cctggtctgt 2640
aaagcccctg caggcaggga cttcttagat agctgcttcc ttagggcatg gcatgtggtg 2700
ggtggttaat gaatggaaga gagggaatga gtgatcaagg gagggaggag ggagtggagt 2760
ggagatttct catcctttcc tgttaattta tgacatcctc ctgcctatga gtccttgact 2820
ctggagtttt acaaagcagt cacatttcaa ataaaagtct gggaaagcaa cacatcatcg 2880
ccaactttta attttgctaa ataaggatat tag 2913
<210>37
<211>1466
<212>DNA
<213>人
<400>37
agccccaagc ttaccacctg cacccggaga gctgtgtgtc accatgtggg tcccggttgt 60
cttcctcacc ctgtccgtga cgtggattgg tgctgcaccc ctcatcctgt ctcggattgt 120
gggaggctgg gagtgcgaga agcattccca accctggcag gtgcttgtgg cctctcgtgg 180
cagggcagtc tgcggcggtg ttctggtgca cccccagtgg gtcctcacag ctgcccactg 240
catcaggaac aaaagcgtga tcttgctggg tcggcacagc ctgtttcatc ctgaagacac 300
aggccaggta tttcaggtca gccacagctt cccacacccg ctctacgata tgagcctcct 360
gaagaatcga ttcctcaggc caggtgatga ctccagccac gacctcatgc tgctccgcct 420
gtcagagcct gccgagctca cggatgctgt gaaggtcatg gacctgccca cccaggagcc 480
agcactgggg accacctgct acgcctcagg ctggggcagc attgaaccag aggagttctt 540
gaccccaaag aaacttcagt gtgtggacct ccatgttatt tccaatgacg tgtgtgcgca 600
agttcaccct cagaaggtga ccaagttcat gctgtgtgct ggacgctgga cagggggcaa 660
aagcacctgc tcgggtgatt ctgggggccc acttgtctgt aatggtgtgc ttcaaggtat 720
cacgtcatgg ggcagtgaac catgtgccct gcccgaaagg ccttccctgt acaccaaggt 780
ggtgcattac cggaagtgga tcaaggacac catcgtggcc aacccctgag cacccctatc 840
aaccccctat tgtagtaaac ttggaacctt ggaaatgacc aggccaagac tcaagcctcc 900
ccagttctac tgacctttgt ccttaggtgt gaggtccagg gttgctagga aaagaaatca 960
gcagacacag gtgtagacca gagtgtttct taaatggtgt aattttgtcc tctctgtgtc 1020
ctggggaata ctggccatgc ctggagacat atcactcaat ttctctgagg acacagatag 1080
gatggggtgt ctgtgttatt tgtggggtac agagatgaaa gaggggtggg atccacactg 1140
agagagtgga gagtgacatg tgctggacac tgtccatgaa gcactgagca gaagctggag 1200
gcacaacgca ccagacactc acagcaagga tggagctgaa aacataaccc actctgtcct 1260
ggaggcactg ggaagcctag agaaggctgt gagccaagga gggagggtct tcctttggca 1320
tgggatgggg atgaagtaag gagagggact ggaccccctg gaagctgatt cactatgggg 1380
ggaggtgtat tgaagtcctc cagacaaccc tcagatttga tgatttccta gtagaactca 1440
cagaaataaa gagctgttat actgtg 1466
<210>38
<211>462
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(116)..(116)
<223>各种碱基
<220>
<221>misc_feature
<222>(197)..(197)
<223>各种碱基
<220>
<221>misc_feature
<222>(334)..(334)
<223>各种碱基
<220>
<221>misc_feature
<222>(402)..(402)
<223>各种碱基
<220>
<221>misc_feature
<222>(429)..(429)
<223>各种碱基
<220>
<221>misc_feature
<222>(438)..(438)
<223>各种碱基
<220>
<221>misc_feature
<222>(443)..(443)
<223>各种碱基
<220>
<221>misc_feature
<222>(459)..(459)
<223>各种碱基
<400>38
taaggtttta taattatttt tatttttctt ttcttttttt tttatggctt ggatgacact 60
ttattttcag atccaatact agaagttgtt tccatgttca cattttcctt cctggnttaa 120
aaaaaagagt tgtatttttt ttttttgctt tttttaaatt atactttaag ttttagggta 180
catgtgcaca acgcagnggt tagctacata tgtatacatg tgccatgttg gcgtgctgca 240
tccagtaact cgtcatttaa cattaggtat atctccaaat gctatccttc cccccattgt 300
atttttcata gcttaaaaat cattgacata ggantaattc caactaaagt acggtattaa 360
atccctgggg gaataaattt tgtcttaaca agggtaaggt tngtgaaaag gatggttttg 420
tcacagggna aaaggganat ccncccattt taaaacccnc ct 462
<210>39
<211>1490
<212>DNA
<213>人
<400>39
ctcgtgcccc ccacggaggg gactgctctc ccccgctgca tcctttctgt gaggtacctt 60
acccacctca gcacctgaga gggtgaaata gaattctaac ctcgacattc gggaagtgtt 120
tttgagaagt ctcggtcggt aagggaagtc ttccaagtcc gtgcagcact aacgtattgg 180
cacctgcctc ctcttcggcc accccccaga tgaggcagct gtgactgtgt caagggaagc 240
cacgactctg accatagtct tctctcagct tccactgccg tctccacagg aaacccagaa 300
gttctgtgaa caagtccatg ctgccatcaa ggcatttatt gcagtgtact atttgcttcc 360
aaaggatcag gggatcaccc tgagaaagct ggtacggggc gccaccctgg acatcgtgga 420
tggcatggct cagctcatgg aagtactttc cgtcactcca actcagagcc ctgagaacaa 480
tgaccttatt tcctacaaca gtgtctgggt tgcgtgccag cagatgcctc agataccaag 540
agataacaaa gctgcagctc ttttgatgct gaccaagaat gtggattttg tgaaggatgc 600
acatgaagaa atggagcagg ctgtggaaga atgtgaccct tactctggcc tcttgaatga 660
tactgaggag aacaactctg acaaccacaa tcatgaggat gatgtgttgg ggtttcccag 720
caatcaggac ttgtattggt cagaggacga tcaagagctc ataatcccat gccttgcgct 780
ggtgagagca tccaaagcct gcctgaagaa aattcggatg ttagtggcag agaatgggaa 840
gaaggatcag gtggcacagc tggatgacat tgtggatatt tctgatgaaa tcagccctag 900
tgtggatgat ttggctctga gcatatatcc acctatgtgt cacctgaccg tgcgaatcaa 960
ttctgcgaaa cttgtatctg ttttaaagaa ggcacttgaa attacaaaag caagtcatgt 1020
gacccctcag ccagaagata gttggatccc tttacttatt aatgccattg atcattgcat 1080
gaatagaatc aaggagctca ctcagagtga acttgaatta tgacttttca ggctcatttg 1140
tactctcttc ccctctcatc gtcatggtca ggctctgata cctgctttta aaatggagct 1200
agaatgcttg ctggattgaa agggagtgcc tatctatatt tagcaagaga cactattacc 1260
aaagattgtt ggttaggcca gattgacacc tatttataaa ccatatgcgt atatttttct 1320
gtgctatata tgaaaaataa ttgcatgatt tctcattcct gagtcatttc tcagagattc 1380
ctaggaaagc tgccttattc tctttttgca gtaaagtatg ttgttttcat tgtaaagatg 1440
ttgatggtct caataaaatg ctaacttgcc agtgattaaa aaaaaaaaaa 1490
<210>40
<211>1677
<212>DNA
<213>人
<400>40
cttgacccta tttatagtgg ctctaaaggt ggtgttatta tgttttctag agcacttcga 60
ttatacaaac gtcaaggaat ccgagttaat gtgctttgcc ctgagtttgt tgaaacagac 120
atgggcacaa tgatcggtcc caaattcctt agtatgatgg ggggctttgt acctatggaa 180
atggtggtga aaggtgcttt tgagctcatc actgatgaga ataaagccgg cgattgccta 240
tggattacta atcggcgagg tcttgagtac tggcccaccc catcagaaga agcaaagtac 300
ttgctgcgtt ctacacgttc caggagaaga actgaataca aagctccacc aattaaacta 360
cctgagagtt ttgagaaaat agttgttcag accttgactc acaactttcg gaatgctacc 420
agtgtagtaa gagcaccact gagattacct atcaaaccaa actatgttct tgtgaagata 480
atctatgctg gtgtaaatgc tagtgatgta aattttagct caggtcgcta ttttggtggc 540
aataacagtg acactgcatc ccgtcttccg tttgatgcag gatttgaggc tgtgggagta 600
attgcagcag ttggggattc tgttactgac ttgaaagttg gcatgccttg tgcgttcatg 660
acttttggag gctatgctga atttacaatg attccttcga aatacgccct tccaatgcct 720
agaccagaac cggaaggtgt tgccatgctt acatcaggat taacagcttc aattgctcta 780
gaaaaggcag gacagatgga atctggaaaa gtggtccttg ttactgctgc ggcaggagga 840
actggtcagt ttgctgttca gcttgcaaaa ttagctggta ataccgtggt tgccacttgt 900
ggaggtgggg caaaggccaa gcttctgaaa gaattgggag tcgacagagt catagactat 960
cacagtgaag atataaaaac ggttctaagg aaagagttcc cgaaaggtat tgatatcatc 1020
tacgaatctg ttggtgggga catgttaaag ttgtgcttgg atgctttggc agtccatgga 1080
cgactcattg tcattggcat gatttctcag tatcaaggag aaaatggttg gacgccatca 1140
aaatatcctg gactatgtga gaagctcttg tcaaagagtc aaactgtggc tggctttttc 1200
ctggtgcaat atagtcacat gtaccaagaa caccttaaca agttatttga cctttactct 1260
tccggaaaac taaaggttgc tgtggatcca aagagattta taggccttca ttctgttgct 1320
gatgctgttg agtatctcca ttcaggcaaa agcgttggga aggtggttgt ctgcgtggac 1380
ccgaccttcg gtcatcaagt agccaaatta tgaatgaaca cggtgtcaaa tacagaaaga 1440
agtgaagttt tcaattctta gtctagagat tgttctcgaa tgttactgaa aatagctgct 1500
agaccagtgc tggaatattt attctcaatg ctttttcaat tttggattac ttgaaagaat 1560
aatccattta tgtataccat gtttatgttt acactataca acaactatga gcagaagaaa 1620
gcgagatatc tacaaaataa attataatcc tttcatttta aaaaaaaaaa aaaaaaa 1677
<210>41
<211>1330
<212>DNA
<213>人
<400>41
atggcgcagt gggacagctt cactgatcaa caggaggaca ctgatagctg ttcagaatct 60
gtgaagtttg atgctcgctc caatacagct ttgcttcccc caaatcctaa aaatggccct 120
ccacttcaag aaaagctgaa atccttcaaa gctgcactga ttgcccttta tctccttgtg 180
tttgctgttc tcattcctat catcgcaata atggcagctc aactcctgaa gtgggaaatg 240
aagaattgca cagttggttc aattaatgca aacagtgtat cctccagtct cctgggaaga 300
ggaaatgaca gtgaagatga agtgagattt cgagaagttg ttatggaaca cattagcaag 360
atggagaaaa gaatccaata tatttcagat actgaagaaa atctcgtaga ttcagagcat 420
tttcaaaatt tcagtgtgac aactgatcaa cgatttgctg atgttcttct ccaactaagt 480
accttggttc ccacagtcca gggacatggg aatgccgtag atgaaatcac caggtcctta 540
ataagtctga ataccacgct gcttgatttg cacctctatg tagaaacact gaatgtcaaa 600
ttccaggaga atacacttaa agggcaagag gaaatcagca aattaaagga gcgtgtgcac 660
aatgcatcag cagaaattat gtctatgaaa gaagaacaag tgcatttgga acaggaaata 720
aaaagagaag tgaaagtcct gaataacatc actaatgatc tcaggctgaa agattgggaa 780
cattctcaga cgttgagaaa tatcacttta attcaaggtc ctcctggacc cccaggagaa 840
aaaggagata gaggtccaac cggagaaagt ggtccaccag gcgttccagg tccagtaggt 900
cctccaggtc ttaagggtga tcgaggatct attggctttc cgggaagtcg aggatatcca 960
ggacaatcag ggaagactgg gaggacagga tatcctggac caaaaggcca aaagggagaa 1020
aaaggcagtg gaagcatcct gactccttct gcgactgtcc gactggttgg tggccgtggc 1080
cctcatgagg gtagagtgga gatattgcac aatggacagt ggggcacagt ttgtgatgat 1140
cactgggaac tgcgtgccgg gcaggttgtc tgcaggagct tgggataccg aggtgttaag 1200
agtgtgcaca agaaagctta ttttggacaa ggtactggtc ccatttggct gaatgaagta 1260
ccctgtttgg ggatggagtc atccattgaa gagtgcaaaa tcagacagtg gggcgtgaga 1320
gtctgttcac 1330
<210>42
<211>431
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(97)..(97)
<223>各种碱基
<220>
<221>misc_feature
<222>(347)..(347)
<223>各种碱基
<220>
<221>misc_feature
<222>(349)..(349)
<223>各种碱基
<220>
<221>misc_feature
<222>(361)..(361)
<223>各种碱基
<220>
<221>misc_feature
<222>(362)..(362)
<223>各种碱基
<220>
<221>misc_feature
<222>(363)..(363)
<223>各种碱基
<220>
<221>misc_feature
<222>(401)..(401)
<223>各种碱基
<220>
<221>misc_feature
<222>(428)..(428)
<223>各种碱基
<400>42
ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60
ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120
gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180
tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240
ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300
ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360
nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420
tcagctcnct g 431
<210>43
<211>669
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(641)..(641)
<223>各种碱基
<400>43
ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60
acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120
gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180
ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240
tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300
ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360
acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420
cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480
tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540
tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600
acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660
aatgaaact 669
<210>44
<211>287
<212>DNA
<213>人
<400>44
gccggagagt ctacaatgtt acccagcatg ctgttggcat tgttgtaaac aaacaagtta 60
agggcaagat tcttgccaag agaattaatg tgcgtattga gcacattaag cactctaaga 120
gccgagatag cttcctgaaa cgtgtgaagg aaaatgatca gaaaaagaaa gaagccaaag 180
agaaaggtac ctgggttcaa ctaaagcgcc acgctgctcc acccagagaa gcacactttg 240
tgagaaccaa tgggaaggag cctgagctgc tggaacctat tccctat 287
<210>45
<211>383
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(147)..(147)
<223>各种碱基
<220>
<221>misc_feature
<222>(309)..(309)
<223>各种碱基
<220>
<221>misc_feature
<222>(349)..(349)
<223>各种碱基
<220>
<221>misc_feature
<222>(365)..(365)
<223>各种碱基
<220>
<221>misc_feature
<222>(372)..(372)
<223>各种碱基
<220>
<221>misc_feature
<222>(380)..(380)
<223>各种碱基
<400>45
ggaacggaaa aggagaattc aagtgtgacc ctcatgaggc aacgtgttat gatgatggga 60
agacatacca cgtaggagaa cagtggcaga aggaatatct cggtgccatt tgctcctgca 120
catgctttgg aggccagcgg ggctcgnctt gtgacaactg ccgcagacct ggggggtgaa 180
cccagtcccg aaggcactac tggccagtcc tacaaccagt attcttcaga gataccattc 240
agagaacaaa cactaatgtt taatttgccc aatttgagtg cttcatgcct tttaggatgt 300
tacaggctng acagagaagg ttttcccgag gagttaaatc atctttttnc catttcccga 360
ggggncaagg cntgtttttn ttt 383
<210>46
<211>523
<212>DNA
<213>人
<400>46
cagaggggca gggcggacgg ctaggagttc aagaaacatc ctggtctgag ggaaaggctg 60
cagctgcacc gccatgaata agcttttcag cttctggaag aggaagaatg agacccgcag 120
ccagggctac aaccttcgag aaaaggattt aaagaaactt cacagagctg cttcagtcgg 180
ggatttgaag aagctgaagg aataccttca gatcaagaaa tatgatgtaa atatgcagga 240
ctatgaatac agaacacctt tgcacctagc ctgtgctaat ggacatacag atgttgtact 300
tttcctaatt gagcaacaat gcaagataaa tgtccgggat agtgaaaaca aatccccatt 360
gattaaggca gtacagtgtc aaaatgagga ttgtgctact attctgctaa actttggtgc 420
agacccagat ctgagggata ttcgttataa tactgttctt cactatgctg tttgtggtca 480
aagtttgtca ttagttgaaa aactgcttga atacgaagct gat 523
<210>47
<211>390
<212>DNA
<213>人
<400>47
tccaaggtca tggcaaaaca tctgaagttc atcgccagga ctgtgatggt acaggaaggg 60
aacgtggaaa gcgcatacag gaccctaaac agaatcctca ctatggatgg gctcattgag 120
gacattaagc atcggcggta ttatgagaag ccatgccgcc gcgacagagg gaaagctatg 180
aaaggtgccg gcggatctac aacatggaaa tggctcgcaa gatcaacttc ttgatgcgaa 240
agaatcgggc agatccgtgg cagggctgct gaggcctgtg ggtgggacac cagtgcgaaa 300
ccctcatcca gttttctctc catctctttt ctttgtacaa tcccatttcc tattaccatt 360
ctctgcaata aactcaaatc acatgtctgc 390
<210>48
<211>669
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(641)..(641)
<223>各种碱基
<400>48
ttcttttgga aaaccaaaca tgctttattt catttttttc acaatttatt taaacatctc 60
acatatacaa aataggtaca atttaatttt tctgcttgcc caagaaacaa agcttctgtg 120
gaaccatgga agaagatgaa aatgagactg gcaaagaaca aatgctgaat ctgaagaaga 180
ggacaacttt gggcaaataa tctgcatact tttaattggg aataagatgg aaaatatgaa 240
tgctaaatca aattttttaa aaaatacacc acacgataca actcaataca ggagtatttc 300
ttctcaaatt cttctagcac catcaacatt cttcaagtat ctgaaatact attaattagc 360
acctttgtat tatgaacaaa acaaaacaag gacctcagtt catctctgtc taggtcagca 420
cctaacaatg tggatcacac tcatgggaaa gtgttttgag gtagtttaaa cctttggaag 480
tttgggtttt aaacttccct ctgtggaaga tattcaaaag ccacaagtgg tgcaaatgtt 540
tatggttttt atttttcaat ttttattttg gttttcttac aaaggttgac atttttcata 600
acaggtgtaa gagtgttgaa aaaaaaattt caatttttgg ngggaacggg ggaaggagtt 660
aatgaaact 669
<210>49
<211>431
<212>DNA
<213>人
<220>
<221>misc_feature
<222>(97)..(97)
<223>各种碱基
<220>
<221>misc_feature
<222>(347)..(347)
<223>各种碱基
<220>
<221>misc_feature
<222>(349)..(349)
<223>各种碱基
<220>
<221>misc_feature
<222>(361)..(361)
<223>各种碱基
<220>
<221>misc_feature
<222>(362)..(362)
<223>各种碱基
<220>
<221>misc_feature
<222>(363)..(363)
<223>各种碱基
<220>
<221>misc_feature
<222>(401)..(401)
<223>各种碱基
<220>
<221>misc_feature
<222>(428)..(428)
<223>各种碱基
<400>49
ctttttatat ttattttcat cgctacacaa acatttttta ggagtttgat tctacctcca 60
ttttggttag atatacaaac tctaccccat gagggantgt atggtgtatt tctagattta 120
gcaacaattt tcttgaaaaa tgtacaatac tatagaaaaa tgaagatagt aaataccagg 180
tataagttaa taacagtgtt tcttttgttc agtaataatg aactgtgtac tagcactgaa 240
ctttaggccc tcctatttgc gtattttctg tttgtatatt tttaaataga ggaattgtga 300
ttataatatt attattttgg aatatcctaa atcataaatt caaaacntna tttagttttt 360
nnnttttttt tttaagatgg agtcccgctt tgtcccaggc nggagtgcag tggcatgatc 420
tcagctcnct g 431
Claims (18)
1.评估结肠癌状态的方法，包括在选自Seq.ID.No.1-33、Seq.ID No.35-36和Seq.ID No.38-41的基因的组合中鉴定每个基因的差异调节(相对于正常群体中相同基因的表达)。
2.根据权利要求1所述的方法，其中在调节的基因的表达中至少有2倍的差异。
3.根据权利要求1所述的方法，其中表明差异调节的p值小于.05。
4.根据权利要求1所述的方法，进一步包括利用不是基于遗传的结肠诊断。
5.根据权利要求1的方法，其中不是基于遗传的癌症标记选自癌胚抗原、CA19-9、CA125、CK-BB和Guanylyl环化酶C。
6.诊断组合体，包括选自Seq.ID.No.1-33、Seq.ID No.35-36和Seq.IDNo.38-41的基因的组合的分离的核酸序列、它们的互补序列或其部分。
7.根据权利要求6所述的诊断组合体，其在适于鉴定其中含有的基因的差异表达的基质中。
8.根据权利要求7所述的诊断组合体，其中所述的基质用于微阵列中。
9.根据权利要求8所述的诊断组合体，其中所述的微阵列是cDNA微阵列。
10.根据权利要求8所述的诊断组合体，其中所述的微阵列是寡聚核苷酸微阵列。
11.诊断组合体，含有选自Seq.ID.Nol-33、Seq.ID No.35-36和Seq.ID No.38-41的基因的组合的分离的核酸序列、它们的互补序列或其部分。
12.诊断结肠癌的试剂盒，含有选自Seq.ID.No.1-33、Seq.ID No.35-36和Seq.ID No.38-41的基因的组合的分离的核酸序列、它们的互补序列或其部分。
13.根据权利要求12所述的试剂盒，进一步包括进行微阵列分析的试剂。
14.根据权利要求12所述的试剂盒，进一步包括一种介质，借助该介质测试所述的核酸序列、它们的互补序列或其部分。
15.评估对结肠癌的治疗应答的方法，包括在选自Seq.ID.No.1-33、Seq.IDNo.35-36和Seq.ID No.38-41的基因的组合中鉴定每个基因的差异调节(相对于正常群体中的相同基因的表达)。
16.根据权利要求15所述的方法，其中应答治疗的评估方法包括确定病人情况是否改善了、没有改善、复发、可能改善或可能复发。
17.评估结肠癌状态的材料，包括选自Seq.ID.No.1-33、Seq.ID No.35-36和Seq.ID No.38-41的基因的组合的分离的核酸序列、它们的互补序列或其部分。
18.评估结肠癌状态的材料，包括选自Seq.ID.No.1-33、Seq.ID No.35-36和Seq.ID No.38-41的基因的组合的分离的核酸序列、它们的互补序列或其部分的表示。
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| WO2006132788A2 (en) * | 2005-06-06 | 2006-12-14 | Genentech, Inc. | Transgenic models for different genes and their use for gene characterization |
| WO2007048074A1 (en) * | 2005-10-21 | 2007-04-26 | Genenews Inc. | Method and apparatus for correlating levels of biomarker products with disease |
| CN101437962A (zh) * | 2006-03-03 | 2009-05-20 | 维里德克斯有限责任公司 | 预测杜克氏b结肠癌复发的分子检测 |
| WO2009052567A1 (en) * | 2007-10-23 | 2009-04-30 | Clinical Genomics Pty. Ltd. | A method of diagnosing neoplasms - ii |
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| WO2013092960A1 (en) | 2011-12-22 | 2013-06-27 | Fundacion Gaiker | Methods and kits for the diagnosis of colorectal cancer |
| CN102586423B (zh) * | 2011-12-27 | 2015-01-07 | 盛司潼 | 一种检测结直肠癌易感基因的方法及试剂盒 |
| CN102586420B (zh) * | 2011-12-27 | 2014-10-22 | 盛司潼 | 一种检测乳腺癌易感基因的方法及试剂盒 |
| CN115678994A (zh) * | 2022-01-27 | 2023-02-03 | 上海爱谱蒂康生物科技有限公司 | 一种生物标志物组合、含其的试剂及其应用 |
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| EP1355151A2 (en) | 2003-10-22 |
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| CN101684500A (zh) | 2010-03-31 |
| EP1355151A3 (en) | 2004-08-25 |
| JP2003325191A (ja) | 2003-11-18 |
| KR100984996B1 (ko) | 2010-10-04 |
| AU2003203561A1 (en) | 2003-10-23 |
| JP4354725B2 (ja) | 2009-10-28 |
| CA2422305C (en) | 2013-07-30 |
| JP2009153521A (ja) | 2009-07-16 |
| AR039211A1 (es) | 2005-02-09 |
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| MXPA03002863A (es) | 2004-08-11 |
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