CN1493261A - 一种组织工程真皮及其制备方法 - Google Patents
一种组织工程真皮及其制备方法 Download PDFInfo
- Publication number
- CN1493261A CN1493261A CNA031345352A CN03134535A CN1493261A CN 1493261 A CN1493261 A CN 1493261A CN A031345352 A CNA031345352 A CN A031345352A CN 03134535 A CN03134535 A CN 03134535A CN 1493261 A CN1493261 A CN 1493261A
- Authority
- CN
- China
- Prior art keywords
- preparation
- culture
- tissue engineered
- dermal equivalent
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 239000011159 matrix material Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 230000002500 effect on skin Effects 0.000 claims description 43
- 210000001519 tissue Anatomy 0.000 claims description 32
- 210000003491 skin Anatomy 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 24
- 210000002950 fibroblast Anatomy 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 17
- 239000012528 membrane Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 210000004207 dermis Anatomy 0.000 claims description 13
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 12
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 12
- 210000002744 extracellular matrix Anatomy 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 229920001778 nylon Polymers 0.000 claims description 10
- 239000004677 Nylon Substances 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 9
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims description 7
- 229920001287 Chondroitin sulfate Polymers 0.000 claims description 7
- 108010035532 Collagen Proteins 0.000 claims description 7
- 102000008186 Collagen Human genes 0.000 claims description 7
- 229960000583 acetic acid Drugs 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 7
- 229940059329 chondroitin sulfate Drugs 0.000 claims description 7
- 229920001436 collagen Polymers 0.000 claims description 7
- 239000012531 culture fluid Substances 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 229920001661 Chitosan Polymers 0.000 claims description 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- 210000000130 stem cell Anatomy 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 4
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 4
- 230000002980 postoperative effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 3
- 102000002734 Collagen Type VI Human genes 0.000 claims description 3
- 108010043741 Collagen Type VI Proteins 0.000 claims description 3
- 229940045110 chitosan Drugs 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 210000003953 foreskin Anatomy 0.000 claims description 3
- 229920002674 hyaluronan Polymers 0.000 claims description 3
- 229960003160 hyaluronic acid Drugs 0.000 claims description 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Substances N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000011177 media preparation Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 229930024421 Adenine Natural products 0.000 claims description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 2
- 102000009016 Cholera Toxin Human genes 0.000 claims description 2
- 108010049048 Cholera Toxin Proteins 0.000 claims description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 2
- 102000002070 Transferrins Human genes 0.000 claims description 2
- 108010015865 Transferrins Proteins 0.000 claims description 2
- 229960000643 adenine Drugs 0.000 claims description 2
- 210000004504 adult stem cell Anatomy 0.000 claims description 2
- 230000000735 allogeneic effect Effects 0.000 claims description 2
- 230000010261 cell growth Effects 0.000 claims description 2
- 238000000502 dialysis Methods 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims description 2
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 2
- 238000001976 enzyme digestion Methods 0.000 claims description 2
- 239000003102 growth factor Substances 0.000 claims description 2
- 229960000890 hydrocortisone Drugs 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 229940125396 insulin Drugs 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 229920002994 synthetic fiber Polymers 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 210000002615 epidermis Anatomy 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 210000001626 skin fibroblast Anatomy 0.000 abstract 3
- 238000013329 compounding Methods 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 14
- 208000027418 Wounds and injury Diseases 0.000 description 14
- 239000000243 solution Substances 0.000 description 12
- 210000004379 membrane Anatomy 0.000 description 7
- 108010023728 Alloderm Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 208000028990 Skin injury Diseases 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 230000003328 fibroblastic effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229920002683 Glycosaminoglycan Polymers 0.000 description 2
- 229920000954 Polyglycolide Polymers 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000000512 collagen gel Substances 0.000 description 2
- 230000000994 depressogenic effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000003725 endotheliocyte Anatomy 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 210000000630 fibrocyte Anatomy 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229920000747 poly(lactic acid) Polymers 0.000 description 2
- 239000004626 polylactic acid Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 206010056340 Diabetic ulcer Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 208000005422 Foreign-Body reaction Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 206010062575 Muscle contracture Diseases 0.000 description 1
- 241000381142 Pachydermia Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 210000002469 basement membrane Anatomy 0.000 description 1
- 239000003397 biobrane Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 125000001246 bromo group Chemical group Br* 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 210000000589 cicatrix Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000011382 collagen catabolic process Effects 0.000 description 1
- 208000006111 contracture Diseases 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000011243 crosslinked material Substances 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 108010007093 dispase Proteins 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000001665 muscle stem cell Anatomy 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000004633 polyglycolic acid Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 239000003351 stiffener Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03134535 CN1232233C (zh) | 2003-09-02 | 2003-09-02 | 一种组织工程真皮的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03134535 CN1232233C (zh) | 2003-09-02 | 2003-09-02 | 一种组织工程真皮的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1493261A true CN1493261A (zh) | 2004-05-05 |
CN1232233C CN1232233C (zh) | 2005-12-21 |
Family
ID=34239978
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 03134535 Expired - Fee Related CN1232233C (zh) | 2003-09-02 | 2003-09-02 | 一种组织工程真皮的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1232233C (zh) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100400655C (zh) * | 2005-12-02 | 2008-07-09 | 王平安 | 组织工程化细胞外基质的制备方法 |
CN101927035A (zh) * | 2010-07-20 | 2010-12-29 | 西南大学 | 以贝壳基质为原料制备人工皮肤的方法 |
CN102091352A (zh) * | 2009-12-09 | 2011-06-15 | 中国人民解放军总医院第一附属医院 | 一种含有汗腺的组织工程皮肤模型的构建方法 |
CN104548209A (zh) * | 2015-02-03 | 2015-04-29 | 苏州磐升生物技术有限公司 | 一种组织工程表皮及其制备方法 |
CN104667353A (zh) * | 2015-03-06 | 2015-06-03 | 广州赛莱拉干细胞科技股份有限公司 | 一种组织工程皮肤及其应用 |
CN104818247A (zh) * | 2015-05-18 | 2015-08-05 | 云南和泽西南生物科技有限公司 | 一种间充质干细胞的培养方法及用途 |
CN105013013A (zh) * | 2015-07-29 | 2015-11-04 | 陕西博与再生医学有限公司 | 一种皮肤溃疡修复基质的制备方法 |
CN105214129A (zh) * | 2015-10-23 | 2016-01-06 | 陕西艾尔肤组织工程有限公司 | 一种生物敷料的制备方法及生物敷料 |
CN105492033A (zh) * | 2013-07-25 | 2016-04-13 | T.C.Ege大学 | 提供组织修复的含微颗粒的具有协同作用的真皮基质及其生产方法 |
CN105597150A (zh) * | 2016-01-27 | 2016-05-25 | 深圳爱生再生医学科技有限公司 | 用于修复皮肤烧伤的皮肤贴片及其制备方法 |
CN107287152A (zh) * | 2017-06-18 | 2017-10-24 | 广东博溪生物科技有限公司 | 用于化妆品抗衰检测的双层皮肤模型的构建方法及培养液 |
CN109529106A (zh) * | 2018-11-14 | 2019-03-29 | 山东隽秀生物科技股份有限公司 | 一种慢性创面修复基质的制备方法 |
-
2003
- 2003-09-02 CN CN 03134535 patent/CN1232233C/zh not_active Expired - Fee Related
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100400655C (zh) * | 2005-12-02 | 2008-07-09 | 王平安 | 组织工程化细胞外基质的制备方法 |
CN102091352A (zh) * | 2009-12-09 | 2011-06-15 | 中国人民解放军总医院第一附属医院 | 一种含有汗腺的组织工程皮肤模型的构建方法 |
CN101927035A (zh) * | 2010-07-20 | 2010-12-29 | 西南大学 | 以贝壳基质为原料制备人工皮肤的方法 |
CN105492033A (zh) * | 2013-07-25 | 2016-04-13 | T.C.Ege大学 | 提供组织修复的含微颗粒的具有协同作用的真皮基质及其生产方法 |
CN105492033B (zh) * | 2013-07-25 | 2019-01-18 | 真皮制药保健品和化妆品工贸股份有限公司 | 提供组织修复的含微颗粒的具有协同作用的真皮基质及其生产方法 |
CN104548209A (zh) * | 2015-02-03 | 2015-04-29 | 苏州磐升生物技术有限公司 | 一种组织工程表皮及其制备方法 |
CN104548209B (zh) * | 2015-02-03 | 2017-01-25 | 苏州磐升生物技术有限公司 | 一种组织工程表皮及其制备方法 |
CN104667353A (zh) * | 2015-03-06 | 2015-06-03 | 广州赛莱拉干细胞科技股份有限公司 | 一种组织工程皮肤及其应用 |
CN104818247B (zh) * | 2015-05-18 | 2017-06-16 | 云南和泽西南生物科技有限公司 | 一种间充质干细胞的培养方法及用途 |
CN104818247A (zh) * | 2015-05-18 | 2015-08-05 | 云南和泽西南生物科技有限公司 | 一种间充质干细胞的培养方法及用途 |
CN105013013A (zh) * | 2015-07-29 | 2015-11-04 | 陕西博与再生医学有限公司 | 一种皮肤溃疡修复基质的制备方法 |
CN105013013B (zh) * | 2015-07-29 | 2020-01-21 | 广东博与再生医学有限公司 | 一种皮肤溃疡修复基质的制备方法 |
CN105214129A (zh) * | 2015-10-23 | 2016-01-06 | 陕西艾尔肤组织工程有限公司 | 一种生物敷料的制备方法及生物敷料 |
CN105214129B (zh) * | 2015-10-23 | 2018-10-30 | 陕西艾尔肤组织工程有限公司 | 一种生物敷料的制备方法及生物敷料 |
CN105597150A (zh) * | 2016-01-27 | 2016-05-25 | 深圳爱生再生医学科技有限公司 | 用于修复皮肤烧伤的皮肤贴片及其制备方法 |
CN107287152A (zh) * | 2017-06-18 | 2017-10-24 | 广东博溪生物科技有限公司 | 用于化妆品抗衰检测的双层皮肤模型的构建方法及培养液 |
CN107287152B (zh) * | 2017-06-18 | 2021-03-19 | 广东博溪生物科技有限公司 | 用于化妆品抗衰检测的双层皮肤模型的构建方法及培养液 |
CN109529106A (zh) * | 2018-11-14 | 2019-03-29 | 山东隽秀生物科技股份有限公司 | 一种慢性创面修复基质的制备方法 |
CN109529106B (zh) * | 2018-11-14 | 2021-09-10 | 山东隽秀生物科技股份有限公司 | 一种慢性创面修复基质的制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN1232233C (zh) | 2005-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Kearney | Clinical evaluation of skin substitutes | |
Hurd et al. | Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells | |
CN101361990B (zh) | 一种双层人工皮肤及其制备方法 | |
Fang et al. | Clinical application of cultured epithelial autografts on acellular dermal matrices in the treatment of extended burn injuries | |
CN100400655C (zh) | 组织工程化细胞外基质的制备方法 | |
CN105013013B (zh) | 一种皮肤溃疡修复基质的制备方法 | |
El Blidi et al. | Extraction methods, characterization and biomedical applications of collagen: A review | |
CN1232233C (zh) | 一种组织工程真皮的制备方法 | |
CN109821071B (zh) | 一种基于脱细胞真皮基质的水凝胶及其制备方法 | |
KR20100046037A (ko) | 생체 내에서 유강장기 또는 유강장기의 일부분의 재생을 증진하기 위한 보철기구 | |
CN101773687B (zh) | 一种复合软组织补片的制备方法 | |
CN104055795A (zh) | 一种可注射植入剂及其制备方法 | |
EP1115432A1 (en) | Dermal scaffold using neutralized chitosan sponge or neutralized chitosan/collagen mixed sponge | |
CN101062429A (zh) | 一种组织工程化双层皮肤的构建方法与应用 | |
Phillips | Biologic skin substitutes | |
CN101062428A (zh) | 一种组织工程化皮肤的构建方法与应用 | |
WO2021012677A1 (zh) | 一种仿生的预脉管化材料及其制备方法和应用 | |
CN105169494A (zh) | 一种组织工程皮肤的制备方法 | |
CN100402097C (zh) | 琼脂/胶原皮肤创伤修复敷料及其制备方法和应用 | |
CN1879578A (zh) | 一种具有生物活性的人工角膜的制备方法 | |
JP3686068B2 (ja) | 皮膚の分離無細胞化方法、無細胞化真皮マトリックス及びその製造方法並びに無細胞化真皮マトリックスを用いた複合培養皮膚 | |
CN1363398A (zh) | 以壳多糖或其衍生物为基质网架的复层人工皮肤 | |
JP4344112B2 (ja) | 生体組織様構造体、骨髄幹細胞の培養方法および培養用キット | |
KR100644078B1 (ko) | 양막과 생분해성 고분자로 구성된 이식용 진피대체물,이의 제조방법 및 용도 | |
CN114591419B (zh) | 一种热水解蛋白及其制备方法和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: SHAANXI AIERFU BIOENGINEERING CO., LTD. Effective date: 20140804 Owner name: SHAANXI AIERFU ACTIVTISSUE ENGINEERING CO., LTD. Free format text: FORMER OWNER: STOMATOLOGICAL HOSPITAL, MILITARY SURGEON UNIV. NO.4, PLA Effective date: 20140804 |
|
C41 | Transfer of patent application or patent right or utility model | ||
CI01 | Correction of invention patent gazette |
Correction item: Second applicant Correct: Shaanxi Eyre skin bioengineering Co., Ltd. False: Shaanxi Aierfu Bioengineering Co., Ltd. Number: 51 Volume: 21 |
|
CI03 | Correction of invention patent |
Correction item: Second applicant Correct: Shaanxi Eyre skin bioengineering Co., Ltd. False: Shaanxi Aierfu Bioengineering Co., Ltd. Number: 51 Page: The title page Volume: 21 |
|
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 710032 XI'AN, SHAANXI PROVINCE TO: 710075 XI'AN, SHAANXI PROVINCE |
|
ERR | Gazette correction | ||
RECT | Rectification | ||
TR01 | Transfer of patent right |
Effective date of registration: 20140804 Address after: High tech Zone in Shaanxi province Xi'an gazelle road 710075 No. 4 Patentee after: Shaanxi Aierfu ActivTissue Engineering Co., Ltd. Address before: 710032 Shaanxi province Xi'an City Rehabilitation Road No. 7 Patentee before: Stomatological Hospital, Military Surgeon Univ. No.4, PLA Patentee before: Shaanxi Eyre skin bioengineering Co., Ltd. |
|
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20051221 Termination date: 20190902 |