CN1390441A - Method for cultivating Se-enriched garlics and garlic bolts - Google Patents
Method for cultivating Se-enriched garlics and garlic bolts Download PDFInfo
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Abstract
A method for cultivating Se-enriched garlic cloves and garlic bolts features that beside conventional cultivation steps including sowing, thinning out seedlings, applying fertilizer, etc or use of water culture procedure, the sodium selenite (or selenate) and nutritive liquid are mixed to obtain the liquid selenium fertilizer, which has different concentrations of inorganic selenium for different growth phases. Said garlic cloves and garlic bolts feature their health-care function.
Description
Technical field
The present invention relates to the domestication of plants method of a kind of garlic, garlic stems, particularly relate to a kind of selenium-enriched garlic and rich selenium garlic stems domestication of plants method.
Background technology
China Preventive Medicial Science Institute's research in recent years and external report show that selenium-enriched garlic can more effectively prevent diseases such as cancer of the stomach, breast cancer, atherosclerotic than common garlic, as " Mammarycancer prevention by regular garlic and selenium-enriched garlic " such as document 1:Ip.C.. described in (Nutrition and Cancer.1992 the 17th volume the 3rd phase 279-286 page or leaf): selenium is the material base that improves garlic diseases prevention activity in the selenium-enriched garlic, simultaneously, selenium also shows hypotoxicity in the selenium-enriched garlic.This shows that selenium-enriched garlic is the carrier of the natural selenium compound of high-efficiency low-toxicity.Therefore, in the selenium-enriched garlic production process, keep it necessarily to contain the selenium level, just can bring into play the wherein health care and the pharmacy effect of selenium.The domestication of plants method of the selenium-enriched garlic described in the document 1 is: be the acyclic water planting method of high selenium of unique selenium source with sodium selenite.Cultivate the shortcoming of (rich selenium) garlic in the method: in whole garlic vegetative period, all supply with high selenium culture fluid, do not consider that the garlic different growing stages absorbs accumulation selenium characteristic and anti-selenium ability, cause seedling phase selenosis, garlic output significantly reduces, selenium can not make full use of, and can cause environmental pollution after discharging with culture fluid.Once found during our high at home selenium area sampling analysis, use the high selenium coal earthenware rock ballast after the burning all the year round in a large number or make garlic, the garlic stems selenium content gathered in the crops on the soil of fertilizer with the smoked soil of high selenium coal earthenware stone also higher, this cultivation (rich selenium) garlic method has the shortcoming that is difficult to reach health care or medicinal Se content level; And concentrate the selenium gas that contains that discharges to be detrimental to health in the time of in this method implementation process, will burning high selenium coal earthenware stone, local peasant once endured the torment of the diseases such as piptonychia alopecia that selenosis brings to the fullest extent, thereby, in recent years, most of high selenium area has stopped with local coal earthenware stone heating or smoked soil preparation fertilizer.
Summary of the invention
The objective of the invention is in order to overcome the shortcoming of prior art, in order to obtain a kind of content that contains selenium-enriched garlic, the rich selenium garlic stems of high activity organic selenium and improve selenium in the big garlic product; In order to improve the output of every mu of selenium-enriched garlic; And be the selenium resources advantage in the area that soil and gangue selenium content are the highest in the world in order sufficiently and reasonably to utilize bestow favour area and Ziyang County, Shaanxi Province, China Hubei Province, in conjunction with the feature of adsorption by soil selenium and garlic absorption selenium; In order to protect environment to bring benefit to the people, thereby provide the selenium-enriched garlic that a kind of cultivation contains the high activity organic selenium and the cultivating method of rich selenium garlic stems.
The object of the present invention is achieved like this: cultivation provided by the invention contains the selenium-enriched garlic of high activity organic selenium and the cultivating method of rich selenium garlic stems, cultivation program by the implantation time sowing of common garlic, garlic stems, thinning, fertilising is carried out, adopt common solution culture facilities and common water planting technology to cultivate (comprise in the mill water culture nutrient solution technologies such as bubbling air, keep garlic throughout the growing season to supply the water planting condition of the sufficient supplies of the required oxygen of its root growth); Or adopt common soil cultivation condition, it is characterized in that: impose at the different growing stage of garlic, garlic stems and comprise that sodium selenite or sodium selenate and mill water culture nutrient solution are made into the water planting liquid that contains the inorganic selenium variable concentrations and make single selenium fertilizer, perhaps mix with high selenium coal earthenware stone that contains the absorbable selenium-containing compound of plant or soil and make selenium fertilizer and cultivate the selenium-enriched garlic that contains the high activity organic selenium, rich selenium garlic stems with sodium selenite or sodium selenate; Wherein being made into the water planting liquid that contains inorganic selenium with sodium selenite or sodium selenate and common mill water culture nutrient solution makes single selenium fertilizer and cultivates the method for selenium-enriched garlic and rich selenium garlic stems and may further comprise the steps:
(1) impose to mix with mill water culture nutrient solution with sodium selenite or sodium selenate in the seedling phase and be made into concentration and for containing inorganic selenium Se amount be: the inorganic selenium mill water culture nutrient solution of 0.1-50mg/L is made selenium fertilizer, and this inorganic selenium mill water culture nutrient solution adopts mode of changing in a week once;
(2) apply fertilizer in the plant fast growing period, impose to mix with mill water culture nutrient solution to be made into sodium selenite or sodium selenate and contain inorganic selenium Se amount and be: the inorganic selenium mill water culture nutrient solution of 10-150mg/L concentration is made selenium fertilizer, and this selenium rich water cultivation nutrient fluid adopts a week to change mode of a culture fluid;
(3) in the fertilising of flower tooth idiophase, impose and contain the inorganic selenium mill water culture nutrient solution that sodium selenite or sodium selenate inorganic selenium mix with mill water culture nutrient solution and make selenium fertilizer, this selenium fertilizer is made into sodium selenite or sodium selenate and common mill water culture nutrient solution and contains inorganic selenium Se amount and be: 10-100mg/L; Adopt a week to change the mode of a culture fluid;
(4) apply fertilizer behind bolting, impose to mix with mill water culture nutrient solution to be made into sodium selenite or sodium selenate and contain inorganic selenium Se amount and be: the inorganic selenium mill water culture nutrient solution of 0-50mg/L concentration is made selenium fertilizer, adopts a week to change the mode cultivation and production garlic and the garlic stems of a culture fluid.
In addition, what more swap out contains the inorganic selenium mill water culture nutrient solution, makes mill water culture nutrient solution be adjusted to the required selenium concentration that contains through adding sodium selenite or sodium selenate again, also can be recycled.
Though this method is unique selenium fertilizer with sodium selenite or sodium selenate, only need on existing solution culture facilities, carry out, but can obtain the high garlic of selenium content (the bright sample of 50-300mg/kg), and be easy to control product and contain the selenium level, be suitable for the high selenium garlic of developed area and production specific use (as medicinal) time to select for use.
In addition, also comprise according to the above-mentioned inorganic selenium mill water culture nutrient solution that contains and mixing with high selenium gangue, its mixed proportion is 1: 10-1: 100 (by weight), with circulating water such as running water or all water that reaches the irrigation water quality can or mill water culture nutrient solution flow through this high selenium colliery powder, inorganic selenium in the high selenium gangue is generated by the circulating water stripping and contains the selenium mill water culture nutrient solution, the mill water culture nutrient solution selenium content that contains inorganic selenium that is produced to reach above-mentioned with sodium selenite or sodium selenate be the fertile preparation of single selenium the water planting liquid that contains inorganic selenium contain the selenium level, and supply the selenium mode to adopt the above-mentioned processing step that contains the inorganic selenium mill water culture nutrient solution, promptly cooperate again and utilize high selenium gangue as main selenium rich water training production selenium-enriched garlic and garlic stems.High selenium gangue mainly contains inorganic selenium-containing compound, and high selenium gangue selenium fertilizer contains selenium Se amount＞20mg/kg gangue from high selenium gangue rock ore deposit, Enshi, China high selenium zone Hubei Province, through pulverizing, crosses the 2-5mm sieve series and becomes high selenium colliery powder selenium fertilizer; At each growth and development stage initial stage of garlic, measure the culture fluid selenium content, when its selenium content is lower than in the adjusting pattern content, results of regular determination culture matrix and plant sample selenium content.Supply for auxiliary selenium fertilizer with sodium selenite or sodium selenate, spray in (spray with containing the selenium aqueous solution and contain 0-1% (volume ratio) vegetables and use surface active agent, the amount of spraying 100-200L/ mu) or the additional cultivation nutrient fluid of intaking, its consumption is 0.1-50mgSe/L.This method replaces sodium selenite with high selenium coal earthenware stone, and cost descends.Be easy to control garlic selenium content (50-100mg/kg).Compare with the earth culture method, because of reducing the absorption of soil to selenium, the availability height of selenium fertilizer.
The invention has the advantages that:
1. the mill water culture nutrient solution that contains inorganic selenium provided by the invention is made selenium fertilizer, use common solution culture facilities can obtain the high garlic of selenium content (the bright sample of 50-300mg/kg), and be easy to control product and contain the selenium level, be suitable for the high selenium garlic of developed area and production specific use (as medicinal) time to select for use.
2. the method for the selenium-enriched garlic of cultivating with the present invention is not only protected environment but also is brought benefit to the people; the selenium-enriched garlic of cultivating with the present invention and the spectrogram of the main selenium-containing compound of garlic stems are shown in Fig. 1-4; inhibitory action and other performance to six kinds of human cancer cells are as shown in table 4, see the inhibitory action to six kinds of human cancer cells from figure and table significantly.
3. this method is simple, is easy to promote.
4. cultivating method provided by the invention is in nonacid soil, and adopting with sodium selenite or sodium selenate is unique selenium fertilizer, adopts single spraying method, the secondary pollution environment of avoiding the leaching loss because of the selenium that is manured into soil (high selenium gangue) to cause.
5. the present invention's selenium-enriched garlic of cultivating has not only kept the health care of common garlic, and it strengthens and widened the health-care efficacy of garlic because of having accumulated selenium-containing compound, the comparison of selenium-enriched garlic and common some functions of garlic as can be seen from table 1-4.
6. the present invention can utilize also that the solubility selenium-containing compound is an inorganic states selenium feature in the high selenium coal earthenware stone, obtaining with high selenium coal earthenware stone is main selenium fertilizer, combination is with sodium selenite or sodium selenate selenium mill water culture nutrient solution again, water planting or earth culture (non-high selenium area and high selenium area) are produced the selenium-enriched garlic method, adopting natural high selenium coal earthenware stone to replace sodium selenite or sodium selenate is main selenium fertilizer, has reduced the selenium-enriched garlic production cost, makes full use of China's selenium resource, has avoided environmental pollution.
Description of drawings
Fig. 1 is a kind of main selenium-containing compound ESI-MS spectrogram in the present invention's selenium-enriched garlic of cultivating;
Fig. 2 is a kind of main selenium-containing compound (Fig. 1) ESI-MS-MS spectrogram in the present invention's selenium-enriched garlic of cultivating;
Fig. 3 is a kind of main selenium-containing compound ESI-MS spectrogram (MS=273) in the present invention's selenium-enriched garlic of cultivating;
Fig. 4 is a kind of main selenium-containing compound (Fig. 3) ESI-MS-MS spectrogram in the present invention's selenium-enriched garlic of cultivating;
Embodiment
Embodiment 1:
Adopt common solution culture facilities, to do selenium fertile be that 40% chemical pure sodium selenate mixes with selenium content with selenium Huo Gelan (Hoagland) the water planting complete nutrition liquid that contains that contains sodium selenate, get the 2.5mg sodium selenate and be dissolved in 1 liter of Huo Gelan (Hoagland) water planting complete nutrition liquid, what can be mixed with selenium content and be 1mg/L contains selenium Huo Gelan (Hoagland) water planting complete nutrition liquid.Impose the water planting liquid of variable concentrations in the garlic different growing stages, it contains selenium Se amount and is followed successively by: seedling phase, 5mg/L (being 12.5mg sodium selenate/1 premium on currency training complete nutrition liquid); The plant fast growing period, 100mg/L (being 250mg sodium selenate/1 premium on currency training complete nutrition liquid); The flower tooth idiophase, 50mg/L (being 125mg sodium selenate/1 premium on currency training complete nutrition liquid); Behind the bolting, 10mg/L (being 25mg sodium selenate/1 premium on currency training complete nutrition liquid); Adopt a week to change and once contain selenium mill water culture nutrient solution mode, cultivation and production garlic and garlic stems.The garlic of results contains the bright garlic of selenium 60.5mgSe/kg, garlic stems selenium content 26.3mgSe/kg.The spectrogram of the main selenium-containing compound of selenium-enriched garlic of cultivating with the present invention is shown in Fig. 1-4.The cancer of the stomach effect that inhibition MNNG brings out rat sees Table 1,2.
Embodiment 2
Cultivation program by the implantation time sowing of common garlic, garlic stems, thinning, fertilising is carried out, comprise to analyze pure sodium selenite and mix with the accurate general mill water culture nutrient solution of commercially available Japanese garden test-object and be made into the water planting liquid that contains inorganic selenium and make single selenium fertilizer, get 2.23mg sodium selenite (selenium content is 45%) and be dissolved in the accurate general mill water culture nutrient solution of 1 liter of Japanese garden test-object, what can be mixed with selenium content and be 1mg/L contains the selenium mill water culture nutrient solution; Adopt common solution culture facilities and common water planting technology to cultivate (comprise in the mill water culture nutrient solution technologies such as bubbling air, keep garlic throughout the growing season to supply the water planting condition of the sufficient supplies of the required oxygen of its root growth); May further comprise the steps:
(1) imposing sodium selenite and the accurate general mill water culture nutrient solution of commercially available Japanese garden test-object in the seedling phase is made into and contains inorganic selenium Se amount and be: 0.1mg/L (being 0.22mg sodium selenite/l premium on currency cultivation nutrient fluid); Adopt a week to change the mode of a selenium rich water cultivation nutrient fluid;
(2) imposing the accurate general mill water culture nutrient solution of sodium selenite and the test-object of Japanese garden in the plant fast growing period is made into and contains inorganic selenium Se amount and be: 10mg/L (being 22.3mg sodium selenite/l premium on currency cultivation nutrient fluid); Adopt a week to change the mode of a culture fluid;
(3), impose the accurate general mill water culture nutrient solution of sodium selenite and the test-object of Japanese garden and be made into and contain inorganic selenium Se and measure and be: 10mg/L (being 22.3mg sodium selenite/l premium on currency cultivation nutrient fluid) in flower tooth idiophase fertilising; Adopt a week to change the mode of a culture fluid;
(4) behind bolting, apply fertilizer, impose the accurate general mill water culture nutrient solution of sodium selenite and the test-object of Japanese garden and be made into and contain inorganic selenium Se amount and be: 5mg/L (being 11.15mg sodium selenite/l premium on currency cultivation nutrient fluid); Adopt a week to change the mode cultivation and production garlic and the garlic stems of a culture fluid.
In addition, what more swap out contains the inorganic selenium mill water culture nutrient solution, makes mill water culture nutrient solution be adjusted to the required selenium concentration that contains through adding sodium selenite or sodium selenate again, also can be recycled.
The spectrogram of the main selenium-containing compound of selenium-enriched garlic of cultivating with present embodiment is shown in Fig. 1-4.Inhibitory action and other performance to six kinds of human cancer cells are as shown in table 4.
Embodiment 3
Cultivation program by the implantation time sowing of common garlic, garlic stems, thinning, fertilising is carried out, imposing sodium selenite and commercially available Huo Gelan (Hoagland) water planting complete nutrition liquid is made into the water planting liquid that contains inorganic selenium and makes single selenium fertilizer, adopt common solution culture facilities and common water planting technology to cultivate (comprise in the mill water culture nutrient solution technologies such as bubbling air, keep garlic throughout the growing season to supply the water planting condition of the sufficient supplies of the required oxygen of its root growth); May further comprise the steps:
(1) imposing sodium selenate and commercially available Huo Gelan (Hoagland) water planting complete nutrition liquid in the seedling phase is made into and contains inorganic selenium Se amount and be: 50mg/L (being 111.5mg sodium selenite/l premium on currency cultivation nutrient fluid); Adopt a week to change the mode of a selenium rich water cultivation nutrient fluid;
(2), impose sodium selenate and commercially available Huo Gelan (Hoagland) water planting complete nutrition liquid and be made into and contain inorganic selenium Se and measure and be: 150mg/L (being 334.5mg sodium selenite/l premium on currency cultivation nutrient fluid) in plant fast growing period fertilising; Adopt a week to change the mode of a culture fluid;
(3), impose sodium selenite and commercially available Huo Gelan (Hoagland) water planting complete nutrition liquid and be made into and contain inorganic selenium Se and measure and be: 100mg/L (being 223mg sodium selenite/l premium on currency cultivation nutrient fluid) in flower tooth idiophase fertilising; Adopt a week to change the mode of a culture fluid;
(4) behind bolting, apply fertilizer, impose sodium selenate and commercially available Huo Gelan (Hoagland) water planting complete nutrition liquid and be made into and contain inorganic selenium Se amount and be: 50mg/L (being 111.5mg sodium selenite/l premium on currency cultivation nutrient fluid); Adopt a week to change the mode cultivation and production garlic and the garlic stems of a culture fluid.
In addition, what more swap out contains the inorganic selenium mill water culture nutrient solution, makes mill water culture nutrient solution be adjusted to the required selenium concentration that contains through adding sodium selenite or sodium selenate again, also can be recycled.
Embodiment 4
Incubation step is with embodiment 3, just sodium selenate and the accurate general mill water culture nutrient solution of Japanese garden test-object be made into the selenium rich water cultivation nutrient fluid that contains inorganic selenium to contain selenium concentration different, wherein (1) imposes in the seedling phase and contains inorganic selenium Se and measure and be: 20mg/L (being 50mg sodium selenate/1 premium on currency cultivation nutrient fluid); (2) in plant fast growing period fertilising, impose and contain inorganic selenium Se amount and be: 70mg/L (being 175mg sodium selenate/l premium on currency cultivation nutrient fluid); (3) in flower tooth idiophase fertilising, impose and contain inorganic selenium Se amount and be: 50mg/L (being 125mg sodium selenate/l premium on currency cultivation nutrient fluid); (4) behind bolting, apply fertilizer, impose and contain inorganic selenium Se amount and be: 20mg/L (being 50mg sodium selenate/l premium on currency cultivation nutrient fluid); The spectrogram of the main selenium-containing compound of selenium-enriched garlic of cultivating with present embodiment is shown in Fig. 1-4.Inhibitory action and other performance to six kinds of human cancer cells are as shown in table 4.
Embodiment 5: at China's high selenium zone, selecting total Se content is 10, the plot of 20mgSe/kg soil each three (0.2 mu/piece), planting garlic preceding 10 days, apply 100 and 60 jin of the high selenium colliery powder selenium fertilizer (unburned) (by 500 and 300 jin of/mu calculating) of selenium content 50mgSe/kg respectively, garlic vegetative period and garlic stems elongating stage (adopting a kind of sedge preceding 15 days) impose 20 jin of (with 100 jin of/mu calculating) selenium fertilizer.Collect garlic stems and garlic ball.The auxiliary respectively 40L that sprays contains 0.3mgSe/L (the 26.8mg sodium selenite is prepared and the sodium selenite liquid of 0.2mgSe/L (the 18mg sodium selenite is separated preparation with 40L is water-soluble) with water-soluble the separating of 40L at garlic fast growing period and flower bud differentiation period.Collect garlic stems and garlic and contain selenium average out to 36mgSe/kg (scope is 32-40) and the bright sample of 43mgSe/kg (scope 38-50mgSe/kg).
The spectrogram of the main selenium-containing compound of selenium-enriched garlic of cultivating with present embodiment is shown in Fig. 1-4.Suppress the high fat of blood effect and see Table 3.
Embodiment 6: at the non-high selenium zone of China, select three 0.2 mu ground (containing selenium 0.79-0.82mgSe/kg soil), flow into soil to irrigate circulating water by high selenium gangue (83.4mgSe/kg gangue) cement filtering ponds are housed.Execute that high selenium gangue amount is in fertile phase of selenium and the cement filtering ponds: garlic vegetative period, bulbil, flower bud differentiation period each 100 jin/time (calculating), garlic stems elongating stage (adopting a kind of sedge preceding 15 days) 150 jin (by 750 jin of/mu calculating) by 500 jin/mu/times and adopt a kind of sedge after 15 days 60 jin (pressing 300 jin of/mu calculating), assist in the garlic fast growing period to spray the sodium selenite liquid (the 26.8mg sodium selenite is separated preparation with 40L is water-soluble) that 40L contains 0.3mgSe/L.The garlic of results and garlic stems selenium content are respectively in 28-32mgSe/kg and the bright sample scope of 19-21mgSe/kg.Table 1 MNNG brings out Wistar rat glandular stomach antrum epithelial cell heteroploid formation rate relatively
#
#N: normal feed group, HG: the common garlic group of high dose, LG: the common garlic group of low dosage, the high sodium selenite group of HSe (selenium content is the 3.0mg/kg feed), the low sodium selenite group (selenium content is the 1.5mg/kg feed) of LSe, HSeG: the rich selenium garlic group (selenium content is the 3.0mg/kg feed) of high dose, LSeG: the rich selenium garlic group (selenium content is the 1.5mg/kg feed) of low dosage.Wherein the garlic amount of adding in HG and the LG group feed is identical with the LSeG group with HSeG respectively, and HSe and LSe group selenium content equate with HSeG and LSeG group respectively.
| Component | ????MNNG(mg/kg) | |||||||
| ????0 | ????10 | ????20 | ????40 | |||||
| Number of animals | Heteroploid formation rate (%) | Number of animals | Heteroploid formation rate (% | Number of animals | Heteroploid formation rate (% | Number of animals | Heteroploid formation rate (% | |
| N | ?5 | ?0 x | ?8 | ?100ay | ?9 | ?100 ay | ?8 | ?100 ay |
| HG | ?7 | ?14 | ?7 | ?71 a | ?7 | ?86 a | ||
| Hse | ?6 | ?16 | ?9 | ?56 b | ?8 | ?63 a | ||
| HseG | ?5 | ?20 | ?10 | ?10 c | ?9 | ?44 a | ||
| LG | ?7 | ?86 a | ||||||
| Lse | ?10 | ?70 a | ||||||
| LseG | ?10 | ?50 b | ||||||
Table 2 GST vigor relatively
#
#N: normal feed group, HG: the common garlic group of high dose, LG: the common garlic group of low dosage, the high sodium selenite group of HSe (selenium content is the 3.0mg/kg feed), the low sodium selenite group (selenium content is the 1.5mg/kg feed) of LSe, HSeG: the rich selenium garlic group (selenium content is the 3.0mg/kg feed) of high dose, LSeG: the rich selenium garlic group (selenium content is the 1.5mg/kg feed) of low dosage.Wherein the garlic amount of adding in HG and the LG group feed is identical with the LSeG group with HSeG respectively, and HSe and LSe group selenium content equate with HSeG and LSeG group respectively.The common garlic of table 3., selenium-enriched garlic and sodium selenite influence group for the horizontal serum total cholesterol serum levels of triglyceride of selenium artery congee sample to Serum TC, TG and AI's
| Component | ????MNNG(mg/kg) | |||||||
| ????0 | ????10 | ????20 | ????40 | |||||
| Number of animals | ?GST | Number of animals | ??GST | Number of animals | ?GST | Number of animals | ?GST | |
| N | ?5 | ?0 x | ?8 | ?100 ay | ?9 | ?100 ay | ?8 | ?100 ay |
| HG | ?7 | ?14 | ?7 | ?71 a | ?7 | ?86 a | ||
| Hse | ?6 | ?16 | ?9 | ?56 b | ?8 | ?63 a | ||
| HseG | ?5 | ?20 | ?10 | ?10 c | ?9 | ?44 a | ||
| LG | ?7 | ?86 a | ||||||
| Lse | ?10 | ?70 a | ||||||
| LseG | ?10 | ?50 b | ||||||
(mgSe/kg feed) be the normal feed control group 0.007 2.10 ± 0.13 of (mmol/L) hardenability value (mmol/L)
a0.43 ± 0.07
a0.65 ± 0.28
aHigh lipid food control group 0.076 3.43 ± 0.65
b0.75 ± 0.12
b4.13 ± 0.62
bHigh lipid food+selenous acid 2.7 2.35 ± 0.26
a0.53 ± 0.13
a2.03 ± 0.58
cSodium group high lipid food+rich selenium garlic 2.7 2.31 ± 0.12
a0.47 ± 0.11
a1.56 ± 0.46
cGroup high lipid food+common garlic 0.076 2.79 ± 0.69
Ab0.66 ± 0.14
b3.59 ± 1.72
bHigh lipid food+the sodium selenite group is identical with high lipid food+rich selenium garlic group selenium content, is the 2.7mgSe/kg feed for group #.High lipid food+common garlic group is identical with the garlic amount that high lipid food+rich selenium garlic group is added, and is 20g garlic powder/kg feed.AI=(serum total cholesterol-high-density lipoprotein (HDL))/high-density lipoprotein (HDL).
Table 4 garlic and selenium-enriched garlic product are to six kinds of human cancer cells' inhibitory action (ED
50)
A
2780(oophoroma), Bel (liver cancer), BGC (cancer of the stomach), HCT (colon cancer), KB (oral epithelium cancer), L
1210(leukemia)
| Human cancer cell's | Product | 1 | Product 2 | |||
| Common garlic | Selenium-enriched garlic | Common garlic | Selenium-enriched garlic | |||
| ?A 2780 | ?2.66×10 -5 | 5.50×10 -6 | ?5.90×10 -4 | ?5.50×10 -4 | ||
| ?Bel | ?1.65×10 -5 | 4.95×10 -6 | ?9.09×10 -4 | ?4.43×10 -4 | ||
| ?BGC | ?2.27×10 -5 | 5.99×10 -6 | ?1.38×10 -3 | ?4.29×10 -4 | ||
| ?HCT | ?8.45×10 -6 | 5.52×10 -6 | ?6.32×10 -4 | ?3.98×10 -4 | ||
| ?KB | ?5.64×10 -5 | 3.15×10 -5 | ?1.12×10 -3 | ?3.23×10 -4 | ||
| ?L 1210 | ?5.40×10 -6 | 1.96×10 -6 | ?3.50×10 -4 | ?8.37×10 -5 | ||
Claims (4)
1, a kind of garlic of rich selenium and cultivating method of rich selenium garlic stems of containing, cultivation program by the implantation time sowing of common garlic, garlic stems, thinning, fertilising is carried out, and adopts common solution culture facilities and common water planting technology to cultivate or adopt common soil cultivation; It is characterized in that: impose at the different growing stage of garlic, garlic stems and comprise that sodium selenite or sodium selenate mix with mill water culture nutrient solution and be made into the water planting liquid that contains the inorganic selenium variable concentrations and make single selenium fertilizer, may further comprise the steps:
(1) impose to mix with mill water culture nutrient solution with sodium selenite or sodium selenate in the seedling phase and be made into concentration and for containing inorganic selenium Se amount be: the inorganic selenium mill water culture nutrient solution of 0.1-50mg/L is made selenium fertilizer, and this inorganic selenium mill water culture nutrient solution adopts mode of changing in a week once;
(2) apply fertilizer in the plant fast growing period, impose with sodium selenite or sodium selenate and mix with mill water culture nutrient solution, be made into and contain inorganic selenium Se amount and be: the selenium mill water culture nutrient solution that contains of 10-150mg/L concentration is made selenium fertilizer, and this selenium rich water cultivation nutrient fluid adopts a week to change mode of a culture fluid;
(3) in flower tooth idiophase fertilising, impose and contain the inorganic selenium mill water culture nutrient solution that sodium selenite or sodium selenate mix with mill water culture nutrient solution and make selenium fertilizer, this selenium fertilizer is made into concentration and measures for containing inorganic selenium Se: 10-100mg/L; Adopt a week to change the mode of a culture fluid;
(4) behind bolting, apply fertilizer, impose with sodium selenite or sodium selenate and mix with mill water culture nutrient solution, be made into concentration for containing inorganic selenium Se amount: the inorganic selenium mill water culture nutrient solution of 0-50mg/L is made selenium fertilizer, adopts a week to change the mode cultivation and production garlic and the garlic stems of a culture fluid.
2. by the described garlic of rich selenium and the cultivating method of rich selenium garlic stems of containing of claim 1, it is characterized in that: also comprise more swap out in a week contain the inorganic selenium mill water culture nutrient solution, again through add sodium selenite or sodium selenate make mill water culture nutrient solution be adjusted to each stage selenium fertile required contain selenium concentration, recycle.
3. by the described garlic of rich selenium and the cultivating method of rich selenium garlic stems of containing of claim 1, it is characterized in that: described selenium fertilizer also comprises according to above-mentioned and contains the inorganic selenium mill water culture nutrient solution or water mixes with high selenium gangue, its mixed proportion is 1: 10-1: 100 (by weight), contain the inorganic selenium mill water culture nutrient solution with what circulating water flow through this high selenium colliery powder, adopt the above-mentioned processing step that contains the inorganic selenium mill water culture nutrient solution and cultivate.
4. by the described garlic of rich selenium and the cultivating method of rich selenium garlic stems of containing of claim 3, it is characterized in that: the described selenium gangue that contains is to contain selenium Se amount＞20mg/kg, through pulverizing, crosses the 2-5mm sieve series and becomes high selenium colliery powder.
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| CN00136760.9 | 2000-12-29 | ||
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| CNB011448644A CN1151709C (en) | 2000-12-29 | 2001-12-27 | Method for cultivating Se-enriched garlics and garlic bolts |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138295B (en) * | 2006-09-05 | 2010-05-12 | 蒋新东 | Main field selenium-rich garlic pedicle, leaf, seed stalk and head cultivating method |
| CN102487802A (en) * | 2011-12-03 | 2012-06-13 | 青岛星牌作物科学有限公司 | Method for culturing selenium-enriched vegetables by alga selenium and polysaccharide water culture process |
| CN102511280A (en) * | 2011-12-19 | 2012-06-27 | 苏州硒谷科技有限公司 | Method of cultivating selenium-enriched garlic plant material |
| CN103766203A (en) * | 2014-01-11 | 2014-05-07 | 金勇� | Production method of penny cress with high selenium content |
| CN104919938A (en) * | 2015-06-30 | 2015-09-23 | 山东省慧福康富硒生物科技有限公司 | Planting method of selenium-rich single-clove garlic |
| CN105961170A (en) * | 2016-05-16 | 2016-09-28 | 苏如伟 | Method for producing blanched garlic leaves with blanched garlic leaf production device |
| CN106358707A (en) * | 2016-08-30 | 2017-02-01 | 宁夏沃福百瑞枸杞产业股份有限公司 | Planting method for organic selenium-rich wolfberry |
| CN106577222A (en) * | 2016-11-29 | 2017-04-26 | 广西南岜仔科技有限公司 | Soilless culture method for selenium-rich garlic |
| CN106613228A (en) * | 2016-12-21 | 2017-05-10 | 王春晓 | Garlic selenium enrichment method |
| CN108207602A (en) * | 2018-01-31 | 2018-06-29 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of selenium-rich sends pool Chinese mesona herb implantation methods |
| CN109422560A (en) * | 2017-08-23 | 2019-03-05 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of ciltivating process of Silicon-rich garlic |
| CN112568075A (en) * | 2020-12-10 | 2021-03-30 | 施恩（恩施）生物医药开发有限公司 | Selenium-rich cultivation method for edible rape bolts and application of selenium-rich cultivation method |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602621B (en) * | 2009-06-25 | 2011-08-10 | 山东农业大学 | Production system of selenium-enriched product based on recycling ecological agriculture |
| CN107926690B (en) * | 2017-11-08 | 2020-08-11 | 河南科技大学 | Three-dimensional circulating flow culture method of selenium-rich leaf vegetables |
-
2001
- 2001-12-27 CN CNB011448644A patent/CN1151709C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138295B (en) * | 2006-09-05 | 2010-05-12 | 蒋新东 | Main field selenium-rich garlic pedicle, leaf, seed stalk and head cultivating method |
| CN102487802A (en) * | 2011-12-03 | 2012-06-13 | 青岛星牌作物科学有限公司 | Method for culturing selenium-enriched vegetables by alga selenium and polysaccharide water culture process |
| CN102511280A (en) * | 2011-12-19 | 2012-06-27 | 苏州硒谷科技有限公司 | Method of cultivating selenium-enriched garlic plant material |
| CN103766203A (en) * | 2014-01-11 | 2014-05-07 | 金勇� | Production method of penny cress with high selenium content |
| CN104919938A (en) * | 2015-06-30 | 2015-09-23 | 山东省慧福康富硒生物科技有限公司 | Planting method of selenium-rich single-clove garlic |
| CN105961170A (en) * | 2016-05-16 | 2016-09-28 | 苏如伟 | Method for producing blanched garlic leaves with blanched garlic leaf production device |
| CN106358707A (en) * | 2016-08-30 | 2017-02-01 | 宁夏沃福百瑞枸杞产业股份有限公司 | Planting method for organic selenium-rich wolfberry |
| CN106577222A (en) * | 2016-11-29 | 2017-04-26 | 广西南岜仔科技有限公司 | Soilless culture method for selenium-rich garlic |
| CN106613228A (en) * | 2016-12-21 | 2017-05-10 | 王春晓 | Garlic selenium enrichment method |
| CN109422560A (en) * | 2017-08-23 | 2019-03-05 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of ciltivating process of Silicon-rich garlic |
| CN108207602A (en) * | 2018-01-31 | 2018-06-29 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of selenium-rich sends pool Chinese mesona herb implantation methods |
| CN112568075A (en) * | 2020-12-10 | 2021-03-30 | 施恩（恩施）生物医药开发有限公司 | Selenium-rich cultivation method for edible rape bolts and application of selenium-rich cultivation method |
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|---|---|
| CN1151709C (en) | 2004-06-02 |
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