CN103849403A - Method for remediating heavy metals in sewage irrigated soil lawn by NTA (nitrilotriacetic acid) and 3-heteroauxin eluting agent - Google Patents
Method for remediating heavy metals in sewage irrigated soil lawn by NTA (nitrilotriacetic acid) and 3-heteroauxin eluting agent Download PDFInfo
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- CN103849403A CN103849403A CN201410122694.6A CN201410122694A CN103849403A CN 103849403 A CN103849403 A CN 103849403A CN 201410122694 A CN201410122694 A CN 201410122694A CN 103849403 A CN103849403 A CN 103849403A
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Abstract
The invention discloses a method for remediating heavy metals in a sewage irrigated soil lawn by an NTA (nitrilotriacetic acid) and 3-heteroauxin eluting agent. The NTA and 3-heteroauxin eluting agent contains a chelating agent nitrilotriacetic acid and 3-heteroauxin are included, wherein the mol ratio of the chelating agent nitrilotriacetic acid to the 3-heteroauxin is 6-12 mmol.kg<-1>:2mg.kg<-1>. The invention also discloses application of the method for remediating heavy metals in soil of a plant body system established in a sewage irrigated region lawn to the promotion of elution and remediation effects of the heavy metals. A compound remediating agent means that 6 mmol.kg<-1> NTA and 3-heteroauxin are used for eluting and remediating sewage irrigated soil.
Description
Technical field
The invention belongs to environmental protection technical field, relate to a kind of NTA of employing and 3-indolyl acetic acid drip washing and repair dirty method of filling with soil grassplot heavy metal.
Background technology
Dirty filling refers to be carried out after harmless treatment city domestic sewage and trade effluent, directly or indirectly recharges for Sewage Irrigation in Farmland, gardens sewage irrigation and groundwater reservoir.China starts to build sewage irrigation engineering in nineteen fifty-seven after the founding of the state, lasts so far over half a century, and China has also passed through relevant dirt filling principle, policy and standard during this period.In sewage, contain a large amount of heavy metal elements, in soil, accumulation can affect absorption and the utilization of plant to nutrient, also the metabolic balance that can upset plant carries out field experiment in Beijing suburb, result shows the dirty plant height, the leaf area index that have affected summer corn of filling with, and its output and dry-matter are obviously reduced.The pollution main manifestations of dirty irrigated area crop quality is that in agricultural-food, heavy metal content exceeds standard and the change of nutritive ingredient.There are some researches show, use each quality parameter of sewage irrigation mud field rice all to irrigate the obvious decline that has in mud field than clear water.Also have research to detect the agricultural-food such as the terraneous potato of soil, beet of sewage irrigation, result shows that wherein in Cd content and the soil solution, Cd content is linear dependence.
Agriculture environmental protection place is investigated the agricultural-food of more than 300 zone of pollution of 24 provinces and cities in the whole nation for 1997, and result shows that wheat, corn heavy metals exceeding standard rate are 15.5%, 14.0%, and heavy metal contamination is mainly take mercury, chromium, cadmium, arsenic etc. as main.Sewage irrigation also can make the protein content of wheat and paddy rice reduce, and along with the prolongation of sewage irrigation duration, the quality of crop can decline year by year.Dirty filling also can significantly be reduced Vitamin C content in vegetables, and the content of other nutritive ingredients is to have to increase to have to subtract.
Heavy metal in sewage is mainly enriched between the 0-20 cm of dirty Irrigated Area Soils top layer.Soil has reduced heavy metal to underground migration to the absorption of heavy metal.There are some researches show long-term dirty area of filling with, local underground water is not subject to the pollution of heavy metal, and in most cases, the heavy metal of dirt in pouring water can't significantly affect underground water generates.Also there are some researches show, the heavy metal in soil especially Cd can show with the increase of leaching time the trend of downward migration, thereby underground water is threatened.In a word, in dirty sewage of filling with, some pollutent is by adsorption by soil, and another part can move to soil lower floor, finally shallow ground water is affected.
Sewage irrigation causes soil and pollution of agricultural products on the one hand, makes pollutent in crop or the middle accumulation of winning, and then enters people by food chain, then causes the sickness rate of various chronic diseases to rise; Long-term dirt filling can cause Groundwater body pollution on the other hand, also can endanger human and livestock health after the daily drinking-water of people or livestock consumption; Also have long sewage irrigation, peasant's physical appearance of direct contact is also had to impact.Edible such agricultural-food for a long time as having indivedual areas, 19 areas such as the Shaoguan in the Ganzhou in Jiangxi and Dayu and Guangdong and the Qujiang River, Hunan, Shaanxi produce the situations of " cadmium rice ", if can work the mischief to the healthy of people.
Owing to directly irrigating with a large amount of raw sewerages, overproof water quality, the dirty area of filling with blindly expands again, causes the pollution of soil and crop and underground water, and sewage irrigation has been the one of the main reasons that causes China's Rural Water Environment to worsen.Sewage irrigation major part is that peasant is spontaneous, and their majority is in the situation that can not get clear water, and spontaneous introducing city life and industry waste water is as water source for irrigation, and this suburb nearby in northern China big and medium-sized cities is very general.
Traditional heavy metal pollution of soil recovery technique has casting landfill method, dilution method, drip-rinsing process, physical partition method and chemical method etc.Compared with traditional processing mode, the major advantage of phytoremediation is that cost is low, and treatment facility is simple, is applicable to large-scale application, is beneficial to the maintenance of soil ecosystem, little to environmental perturbation, and the feature such as possess aesthetic value.
In the biological restoration of heavy metal pollution of soil, application is phytoremediation technology early, more widely.Phytoremediation is exactly the heavy metal contaminants utilizing in plant absorption, accumulation and fixing soil, is transferred to root or over-ground part, then gathers in the crops at suitable growth phase, and after treatment, ashing is reclaimed heavy metal or carried out landfill.The result of study that Herba pteridis vittatae can a large amount of enrichment As of having reported for work first such as Chen Tongbin, analyzed the enriching quantity of this plant Different Organs to heavy metal simultaneously, the content of finding As in Herba pteridis vittatae Different Organs tissue is accessory pinna > petiole > root system, As easily upwards transportation and enrichment in this plant materials is described, demonstrating Herba pteridis vittatae has extremely strong patience and unique accumulation ability to As.Sun etc. study discovery, be grown in containing Cd content in the Sedum alfredii Hance plants stems in Cd slag soil and leaf apparently higher than root, the Sedum alfredii Hance plant root Cd content that is grown in non-Cd mining area is apparently higher than the content in cauline leaf, thereby confirms that Sedum alfredii Hance also has stronger inrichment to Cd.Tian Shengni etc. are by being preced with relatively thinking of grass with goose, and Caulis Miscanthis floriduli is that Cu, Pb, Zn Single Pollution or combined pollution are all had to good repairing effect.Ye Chun and studied reparation and the activating mechanism of alfalfa to Pb contaminated soil, finds out from X-ray micro-zone analysis result, intercellular substance Pb content is the highest, and cell walls and vacuole take second place, minimum in kytoplasm; Pb mainly exists with the form of indissoluble in alfalfa body, and alfalfa is relevant with the formation of plant complexing element to the tolerance of Pb.Because biomass of alfalfa is high, so it is the desirable rehabilitation plant of one that P in soil b pollutes that alfalfa can be used as.Secretions from plant roots can change rhizosphere soil environment, can make Cr, the Hg of multivalence state, valence state and the form of As change, and affects its toxic effect.The root hair of plant can directly increase root table from soil exchange adsorption heavy metal to be fixed.But the growth cycle of the super enriching plant for restoration of soil polluted by heavy metal having is grown, biomass is relatively little, and low for the bioaccumulation efficiency of the low heavy metal of some biological effectiveness, this is restricted single use phytoremediation.
In reality, pollutant in soil is of a great variety, combined pollution is general, and pollution level and difference in thickness are large, and epidermic soil type is many simultaneously, the area differentiation of its composition, character, condition is obvious, and after repairing, the space planning of soil recycling formula requires different.Therefore, individual event recovery technique is toward being difficult to reach reparation target, and exploitation complex repairation pattern just becomes the main direction of studying that soil pollution is repaired.
The main Types that starts now the complex repairation technology that drops into application has the reparation of plant/microbial association, animal/plant combined reparation and chemistry/materialization-Co-bioremediation.The mechanism of plant/microbial association reparation is that higher plant and soil microorganisms often exist synergy in process of growth, on the one hand plant can provide the soil microorganisms required carbon source of growing, simultaneously again through base of leaf to root delivering oxygen, be formed with the microenvironment that is beneficial to oxidation, promote the Decomposition of aerobic microbiological to pollutent.Chemistry/materialization-Co-bioremediation can be brought into play the advantage of short, instant effect of the cycle that chemistry or physics repairs, and in conjunction with nondestructive biological restoration feature, development is contaminated soil restorative procedure the most with potential applications based on chemical-biological recovery technique.As utilize the coordination stripping of organic complexing agent, and increase heavy metal concentration in the soil solution, improve the assimilated efficiency of plant, thereby realize the reparation of strengthening Enhanced phytoextraction.Therefore, improve the method that plant absorbs enriching heavy metal, except using and continuing to find accumulation ability is strong and biomass is large super enriching plant, we can add artificial or natural sequestrant and other enhancements and improve the ability of plant absorption enriching heavy metal in phytoremediation process.
Sequestrant inducing plant extracts remediating heavy metal, and it can increase the solubleness of heavy metal in soil on the one hand, can improve on the other hand heavy metal rhizosphere diffusibility, can also promote heavy metal to transport to overground part from root system.Pealosa etc. have studied the factor that several promotion lupine repairing heavy metal in soil pollute, found that, sequestrant NTA can promote metal ion (Fe, Mn, Cu, Zn, Cd) migration, impel the contained concentration of metal ions of lupine to raise, especially As, Cd and the increase of Pb concentration are more obvious.Qian Meng etc. plant elsholtzia splendens on Cu, Zn, Pb, Cd combined contamination soil, use 5 mmolkg
-1after EDTA, the concentration of Cu, Zn, Pb, Cd improves 27.4,3.2,17.6 and 89.5 times than contrast respectively.The researchs such as Stanhope show, along with the increase of EDTA concentration, the ratio that in soil, the content of Cu, Zn, Cd, Pb, Ni soluble state heavy metal accounts for total heavy metal content is linear raising.In chelation evoked phytoremediation process, the duration of service of sequestrant is very important, generally to gather in the crops in first 1 ~ 2 week and add plant, now growth and development of plants maturation, less on increment impact after processing in the short period of time, the total metals of plant extract can significantly increase.
By warmhouse booth potted plant experiment, research sequestrant and tensio-active agent is independent or the effect of the auxiliary golden good fortune mushroom restoration of soil polluted by heavy metal of Combined Processing.Result shows, the EDTA of high density makes the biomass of Jin Fugu reduce than contrast, the concentration of sporophore Pb, Cu and Cd improves 15 ~ 88 times, 0.8 ~ 3.3 times and 0.5 ~ 0.6 times than contrast respectively. while adding tensio-active agent separately, each biomass of processing does not have significant difference with contrasting, and heavy metal concentration rangeability there is no to add separately the processing of EDTA large.But this conventional complexing agent of EDTA has stronger bio-toxicity, in application, be greatly limited; Therefore, the application of novel chelating agents has been a great concern.
Plant hormone indolylacetic acid (IAA) is alleviated the coercion of heavy metal on plants.If IAA can be alleviated the phytotoxicity of heavy metal chelant, promote root system of plant to extend, increase phytomass, utilize so absorption, transhipment and the accumulation of the collaborative sequestrant plant of IAA to heavy metal, if can improve plant extract efficiency, will have great importance.But utilize the correlation technique of IAA remediating heavy metal to there is no bibliographical information.
Lawn vegetation is checked winds and fixed drifting sand, water conservation, purify air, afforest soil, conserve water and soil and regulate the effects such as miniclimate.Lawn plant has certain using value in addition, and it is the important component part of urban landscaping, can be used for afforesting city, and its adaptability is stronger.Both can avoid food chain if lawn plant is applied to heavy metal-polluted soil reparation, and can also take away the heavy metal in soil, and turf volume can be used further to the greening in city simultaneously.If lawn plant is applied to the dirty native heavy metal reparation of filling with, has certain reparation value and practical value.
Repair dirt with lawn plant and fill with soil, add degradable sequestrant, respectively again using 3-indolyl acetic acid as auxiliary, make the heavy metal of turf establishment system reach the object that drip washing is repaired, this not only can be avoided heavy metal to enter food chain harm humans, also turf volume can be applied to urban afforestation, the stronger reproducibility of lawn plant is also suitable for repairing in addition simultaneously.After the native heavy metal content of dirty filling is up to standard, can continue to be applied to agriculture production.Therefore this use Simulated Acid Rain is carried out drip washing to the soil after repairing, and reaches the object of the drip washing reparation of turf establishment system sewage irrigation district heavy metal pollution of soil.There are some researches show, lawn plant has certain absorption and tolerance to heavy metal-polluted soil.And the technology of NTA being combined to the dirty irrigated area of drip washing reparation turf establishment system heavy metal-polluted soil at present with 3-indolyl acetic acid also there is no bibliographical information.
Summary of the invention
The object of the present invention is to provide a kind of NTA of employing and 3-indolyl acetic acid to repair the method for dirty Irrigated Area Soils heavy metal.The present invention, on the basis of optimization experiment, experimental results show that: use 6 mmolkg
-1the drip washing effect that NTA+3-indolyl acetic acid is filled with native to dirt is best.For realizing this object, the invention provides following technical scheme:
A kind of NTA and 3-indolyl acetic acid eluent, is characterized in that it is made up of sequestrant nitrilotriacetic acid(NTA) and 3-indolyl acetic acid, and wherein the mol ratio of sequestrant nitrilotriacetic acid(NTA) and 3-indolyl acetic acid is 6-12mmolkg-1NTA:3-indolylacetic acid 2mgkg
-1.
The present invention further discloses NTA and 3-indolyl acetic acid and repair the method for dirty Irrigated Area Soils heavy metal, it is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA); 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use.Soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment
4)
2sO
4, Na
2sO
4, K
2sO
4, MgSO
4, Ca (NO
3)
2, Mg (NO
3)
2, H
2sO
4make SO
4 2-, NO
3 -, Cl
-, NH
4 +, Mg
2+, Ca
2+, K
+, Na
+concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL
-1rainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, 0.3g Festuca Arundinacea grass seeds is then planted in cotton and nylon wire sealing for bottom in each pipe, pvc pipe is sling and fixed, and lower end is joined and is put Erlenmeyer flask and access effusion.Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, room temp 18-35 ℃ between incubation period, and relative humidity 40-61%, normal water supply between establishment period, to maintain plant normal growth.Plant-growth 30d, according to experimental design respectively by the complex repairation agent of respective amount, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, gathers in the crops lawn plant over-ground part after processing 10d, cradle second day, carry out acid rain drip washing for the first time, each 30ml(is equivalent to 42mm quantity of precipitation), every 7 days are once, drip washing three times, finally collects leacheate altogether.
(3) mensuration of biomass: gather in the crops lawn plant over-ground part after sowing 40d, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal content in Digestive system with TAS-990 atomic absorption spectrophotometer; Wherein the NTA of respective concentration or 3-indolyl acetic acid refer to wherein one below:
6?mmol·kg
-1NTA；
12?mmol·kg
-1NTA；
6 mmolkg
-1nTA+3-indolyl acetic acid 2mgkg
-1;
12 mmolkg
-1nTA+ 3-indolyl acetic acid 2mgkg
-1.
The present invention further discloses method that eluent repairs dirty irrigated area turf establishment system heavy metal-polluted soil in the application promoting aspect Festuca Arundinacea ground biomass.Wherein said complex repairation agent refers to 6 mmolkg-1NTA+3-indolyl acetic acid dirt is filled with to native drip washing reparation.
The conclusion that the present invention reaches by test: use 6 mmolkg
-1the drip washing repairing effect that NTA+3-indolyl acetic acid is filled with native to dirt is best.
1 development materials and methods
1.1 experiment material
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material.
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and molecular formula is N (CH
2cOOH)
3, be purchased from Chemical Reagent Co., Ltd., Sinopharm Group, be analytical pure.
3-indolyl acetic acid molecular formula is C
10h
9nO
2, be purchased from Tianjin recovery fine chemistry industry institute, be analytical pure.
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use.Soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment
4)
2sO
4, Na
2sO
4, K
2sO
4, MgSO
4, Ca (NO
3)
2, Mg (NO
3)
2, H
2sO
4make SO
4 2-, NO
3 -, Cl
-, NH
+, Mg
2+, Ca
2+, K
+, Na
+concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL
-1rainwater, and with HCl allotment pH be 5.59.
1.2 experimental design
This experiment arranges 5 processing altogether: do not add the contrast (I) of NTA and 3-indolyl acetic acid; 6 mmolkg
-1nTA(II); 12 mmolkg
-1nTA(III); 6 mmolkg
-1nTA+3-indolyl acetic acid 2mgkg
-1(IV); 12 mmolkg
-1nTA+ 3-indolyl acetic acid 2mgkg
-1(V).Each processing repeats for 3 times.
1.3 lawn plants are cultivated
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, 0.3g Festuca Arundinacea grass seeds is then planted in cotton and nylon wire sealing for bottom in each pipe, pvc pipe is sling and fixed, and lower end is joined and is put Erlenmeyer flask and access effusion.Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 18 ~ 35 ℃ of room temps between incubation period, relative humidity 40 ~ 61%.Normal water supply between establishment period, to maintain plant normal growth.Plant-growth 30d, is dissolved in distilled water by the NTA of respective concentration by required dosage respectively according to experimental design, and 3-indolyl acetic acid is dissolved in a small amount of ethanol by required dosage, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast.After processing 10d, gather in the crops lawn plant over-ground part.Cradle second day, carry out acid rain drip washing for the first time, each 30ml(is equivalent to 42mm quantity of precipitation), every 7 days are once, altogether drip washing three times.Finally collect leacheate.
1.4 index determining
The mensuration of biomass: gather in the crops lawn plant over-ground part after sowing 40d, after deionized water rinses repeatedly, put it in baking oven, dry to constant weight under 80 ℃ of conditions, survey its dry weight.
The mensuration of heavy metal content: get leacheate 5mL, use nitric acid and perchloric acid to digest at 120-140 ℃, gained solution is settled to 10mL with distilled water.Finally measure heavy metal (Cd, Cu, Zn) content in Digestive system with TAS-990 atomic absorption spectrophotometer.
1.5 data analysis processing
Adopt SPSS17.0 software to carry out statistical study to data.
2 development results analyses
Table 1 is heavy metal concentration in pvc pipe percolate under different treatment.The interpolation of NTA has improved the concentration of each heavy metal in percolate, illustrates that NTA has also promoted that metal ion moves vertically downward in activation heavy metal.Add separately NTA treatment group compared with Cd, Cu, Zn concentration in same concentration NTA+ indolylacetic acid group effusion, difference all not significantly (
p> 0.05).12 mmolkg
-1in NTA+ indolylacetic acid treatment group effusion, Cd, Cu, Zn concentration are significantly higher than contrast, are 2.38,1.57,5.8 times of contrast.In interpolation high density NTA group, the concentration ratio lower concentration NTA group of each metallic element increases.According to country, about the regulation in groundwater quality standard (GB/T14848-93), III class standard, take HUMAN HEALTH benchmark value as foundation, is mainly applicable to centralized Drinking Water water source and work, agricultural water.The concentration of Groundwater Environmental Quality III class standard Cd, Cu, Zn will be not more than 0.01,1.0,1.0mgL, and in this experiment, in percolate, the concentration of Cd all exceedes III class standard, but Cu, Zn concentration do not exceed standard.
The concentration of heavy metal (ug/mL) in leacheate under table 1 different treatment
| Process | Cd | Cu | Zn |
| Do not add NTA and 3-indolyl acetic acid | 0.029±0.010c | 0.187±0.066c | 0.025±0.012c |
| 6 mmol·kg -1NTA | 0.053±0.012ab | 0.250±0.023abc | 0.077±0.032b |
| 12 mmol·kg -1NTA | 0.068±0.007a | 0.279±0.017ab | 0.128±0.027a |
| 6 mmol·kg -1NTA+3-indolyl acetic acid | 0.042±0.010bc | 0.220±0.022bc | 0.073±0.010b |
| 12 mmol·kg -1NTA+3-indolyl acetic acid | 0.069±0.014a | 0.293±0.007a | 0.145±0.034a |
With the different letter representation significant differences of column data (
p<0.05).
development conclusion
Under finite concentration, the interpolation of IAA can be alleviated heavy metal and the phytotoxicity of sequestrant to Festuca Arundinacea, is conducive to the accumulation of plant to heavy metal; Add that NTA combines interpolation with 3-indolyl acetic acid and can promote the raising of Festuca Arundinacea ground biomass.From rehabilitation cost and effect and the potential risk to groundwater pollution, can consider to use 6 mmolkg
-1nTA+3-indolyl acetic acid is filled with native drip washing reparation to dirt.
Embodiment:
Below in conjunction with specific embodiment, the present invention will be further described, and following each embodiment is not only limitation of the present invention for the present invention is described.Wherein chemical reagent used all has commercially available.
Embodiment 1
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA); 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use.Soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment
4)
2sO
4, Na
2sO
4, K
2sO
4, MgSO
4, Ca (NO
3)
2, Mg (NO
3)
2, H
2sO
4make SO
4 2-, NO
3 -, Cl
-, NH
4 +, Mg
2+, Ca
2+, K
+, Na
+concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL
-1rainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, 0.3g Festuca Arundinacea grass seeds is then planted in cotton and nylon wire sealing for bottom in each pipe, pvc pipe is sling and fixed, and lower end is joined and is put Erlenmeyer flask and access effusion.Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 20 ℃ of room temps between incubation period, and relative humidity 40%, normal water supply between establishment period, to maintain plant normal growth.Plant-growth 30d, according to experimental design respectively by the complex repairation agent of respective amount, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, gathers in the crops lawn plant over-ground part after processing 10d, cradle second day, carry out acid rain drip washing for the first time, each 30ml(is equivalent to 42mm quantity of precipitation), every 7 days are once, drip washing three times, finally collects leacheate altogether.
(3) mensuration of biomass: gather in the crops lawn plant over-ground part after sowing 40d, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal Cd, Cu, Zn content in Digestive system with TAS-990 atomic absorption spectrophotometer; Wherein respective concentration: 6 mmolkg
-1nTA+3-indolyl acetic acid 2mgkg
-1.
Embodiment 2
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA); 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use.Soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment
4)
2sO
4, Na
2sO
4, K
2sO
4, MgSO
4, Ca (NO
3)
2, Mg (NO
3)
2, H
2sO
4make SO
4 2-, NO
3 -, Cl
-, NH
4 +, Mg
2+, Ca
2+, K
+, Na
+concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL
-1rainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, 0.3g Festuca Arundinacea grass seeds is then planted in cotton and nylon wire sealing for bottom in each pipe, pvc pipe is sling and fixed, and lower end is joined and is put Erlenmeyer flask and access effusion.Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 30 ℃ of room temps between incubation period, and relative humidity 501%, normal water supply between establishment period, to maintain plant normal growth.Plant-growth 30d, according to experimental design respectively by the complex repairation agent of respective amount, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, gathers in the crops lawn plant over-ground part after processing 10d, cradle second day, carry out acid rain drip washing for the first time, each 30ml(is equivalent to 42mm quantity of precipitation), every 7 days are once, drip washing three times, finally collects leacheate altogether.
(3) mensuration of biomass: gather in the crops lawn plant over-ground part after sowing 40d, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal Cd, Cu, Zn content in Digestive system with TAS-990 atomic absorption spectrophotometer; Wherein respective concentration: 12 mmolkg
-1nTA+ 3-indolyl acetic acid 2mgkg
-1.
Claims (5)
1. NTA and a 3-indolyl acetic acid eluent, is characterized in that it is made up of sequestrant nitrilotriacetic acid(NTA) and 3-indolyl acetic acid, and wherein the mol ratio of sequestrant nitrilotriacetic acid(NTA) and 3-indolyl acetic acid is 6-12mmolkg
-1nTA:3-indolylacetic acid 2mgkg
-1.
2. adopt NTA and 3-indolyl acetic acid eluent described in claim 1 to repair a dirty method of filling with soil grassplot heavy metal, it is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA); 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use;
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation; (NH for this experiment
4)
2sO
4, Na
2sO
4, K
2sO
4, MgSO
4, Ca (NO
3)
2, Mg (NO
3)
2, H
2sO
4make SO
4 2-, NO
3 -, Cl
-, NH
4 +, Mg
2+, Ca
2+, K
+, Na
+concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL
-1rainwater, and with HCl allotment pH be 5.59;
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, 0.3g Festuca Arundinacea grass seeds is then planted in cotton and nylon wire sealing for bottom in each pipe, pvc pipe is sling and fixed, and lower end is joined and is put Erlenmeyer flask and access effusion;
Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, room temp 18-35 ℃ between incubation period, and relative humidity 40-61%, normal water supply between establishment period, to maintain plant normal growth;
Plant-growth 30d, according to experimental design respectively by the complex repairation agent of respective amount, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, gathers in the crops lawn plant over-ground part after processing 10d, cradle second day, carry out acid rain drip washing for the first time, each 30ml(is equivalent to 42mm quantity of precipitation), every 7 days are once, drip washing three times, finally collects leacheate altogether;
(3) mensuration of biomass: gather in the crops lawn plant over-ground part after sowing 40d, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal content in Digestive system with TAS-990 atomic absorption spectrophotometer; Wherein the NTA of respective concentration or 3-indolyl acetic acid refer to wherein one below:
6?mmol·kg
-1NTA；
12?mmol·kg
-1NTA；
6 mmolkg
-1nTA+3-indolyl acetic acid 2mgkg
-1;
12 mmolkg
-1nTA+ 3-indolyl acetic acid 2mgkg
-1.
3. the method that described in claim 2, eluent is repaired dirty irrigated area turf establishment system heavy metal-polluted soil, heavy metal wherein refers to Cd, Cu, Zn content.
4. the application of the method for NTA and the dirty filling of 3-indolyl acetic acid eluent reparation soil grassplot heavy metal aspect promotion heavy metal drip washing reparation described in claim 2.
5. application claimed in claim 4, wherein said complex repairation agent refers to 6 mmolkg
-1nTA+3-indolyl acetic acid is filled with native drip washing reparation to dirt.
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| CN112934949A (en) * | 2021-02-18 | 2021-06-11 | 上海交通大学 | Lawn restoration method based on heavy metal contaminated soil obtained by harvesting withered and old leaves |
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| CN112574751A (en) * | 2019-09-29 | 2021-03-30 | 中国石油化工股份有限公司 | Ferric-iron-containing heavy metal leaching composition, application thereof and method for repairing heavy metal soil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630474A (en) * | 2012-05-04 | 2012-08-15 | 天津师范大学 | Method for repairing garbage compost heavy metal by adopting NTA (Nitrilotriacetic acid) of waste interlayer |
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2014
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|---|---|---|---|---|
| CN102630474A (en) * | 2012-05-04 | 2012-08-15 | 天津师范大学 | Method for repairing garbage compost heavy metal by adopting NTA (Nitrilotriacetic acid) of waste interlayer |
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| Title |
|---|
| 周建民等: "《3－吲哚乙酸协同螯合剂强化植物提取重金属的研究》", 《环境科学》 * |
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| CN112934949A (en) * | 2021-02-18 | 2021-06-11 | 上海交通大学 | Lawn restoration method based on heavy metal contaminated soil obtained by harvesting withered and old leaves |
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