CN102511280A - Method of cultivating selenium-enriched garlic plant material - Google Patents
Method of cultivating selenium-enriched garlic plant material Download PDFInfo
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Abstract
The invention discloses a method of cultivating a selenium-enriched garlic plant material. The selenium content of the selenium-enriched garlic plant material is 3mg/kg-1500mg/kg, and selenium existing in a form of Se-methylselenocysteine accounts for more than 80% of the content of total selenium. The method is characterized by including: adding a selenium source into garlic growth medium in growth of garlic, using the selenium source to adjust the selenium content of the growth medium to be 0.2-100mg/kg, and adding alkaline matter to adjust the overall or local environmental pH value of the growth medium to be 7-8. By the method, the selenium source is obtained from different selenium concentration gradients, a good selenium form result is obtained, and the shortages that persistence of plant material selenium in the prior art is poor, the selenium is easy to be reduced in the environment, the utilization rate of the selenium is limited and the like are overcome.
Description
Technical field
The present invention relates to utilize Plant Transformation to produce the method for methylselenocysteinefrom, particularly a kind of method of utilizing high selenium garlic to produce methylselenocysteinefrom.
Background technology
Selenium is trace element essential in the humans and animals body, is to constitute the nearly activated centre of 23 kinds of functional proteins such as glutathione peroxidase (GSH-Px), selenoprotein P, have anticancer, anti-oxidant, improve effect such as immunity.To the greatest extent China has 72% area day selenium intake significantly to be lower than the selenium daily intaking amount that The World Health Organization (WHO) is recommended approximately, has caused serious human body and animal health problem.
Selenium totally is divided into inorganic selenium and organic selenium two big classes: the former comprises selenate, selenite etc., and inorganic selenium assimilation effect for humans and animals is poor, and risk of toxicity is big, and improper use very easily causes selenosis; The latter mainly is meant the selenium with the biological organic molecule combining form, and the organic selenium absorption ratio is high concerning humans and animals, and risk of toxicity is little, is more suitable for mending the requirement of selenium.
The further segmentation of organic selenium mainly comprises selenomethionine, selenocysteine, methylselenocysteinefrom, also is included in all seldom selenium polysaccharides, seleno nucleic acid etc. of content in the animals and plants.And also there is bigger difference in the metabolism of former three in the humans and animals body, and main non-specific binding stored the long-term long-acting biological function of performance as selenium after methylselenocysteinefrom got into body in proteosome; Methylselenocysteinefrom then directly gets in the metabolic pathway of synthesizing of selenoprotein, for selenium biology merit has been brought into play direct effect effect (progress of yeast organic selenium, Chen Min, Li Feng, feed research, 2010).Methylselenocysteinefrom then is different from other several kinds of seleno-amino acids, is considered to one of effective chemical anticancerogenics ((SMT) gene clone of broccoli selenium methyltransferase and overexpression research, Li Bin, University Of Agriculture and Forestry In Fujian's Master's thesis, 2009).
At present, the exploitation of rich selenium product and associated materials increases gradually, and method is also varied, but does not all also relate to the rich selenium material that specific selenium form is rich in directive breeding.Like application number is the production method that 200810017759.5 Chinese invention patent application discloses a kind of organic selenium; It utilizes the growth of plant that inorganic selenium is converted into organic selenium; After the seed of plant soaked with the mineral salt sodium selenite solution again through germination, plantation; Growth through plant is converted into organic selenium with inorganic selenium, just becomes organic selenium product through handling then.Its kind is limited to common mung bean, cowpea, tomato, cucumber, and these kinds are limited and form is uncertain to the accumulation of selenium, can not realize being rich in the production of specific modality seleno-amino acids material.
Garlic is a kind of high accumulator plant of selenium; Under natural or artificial rich selenium condition; Selenium-enriched garlic can reach higher Se content; And the selenium in the selenium-enriched garlic mainly exists with the form of methylselenocysteinefrom, to cancer have the excellent prevention effect (methylselenocysteinefrom synthetic with split research Liu Jianqun, Liu Fang; Zhang Xiaoping; Chemical research and application, 2008) Chinese invention patent " contains the garlic of rich selenium and the cultivating method of rich selenium garlic stems " (application number 01144864.4) discloses a kind of garlic of rich selenium and cultivating method of rich selenium garlic stems of containing, and this method obtains the high garlic of selenium content (the bright appearance of 50-300mg/kg) through add sodium selenite or sodium selenate in the normal cultivation program of garlic, garlic stems.But; This method has been used a large amount of sodium selenites or sodium selenate under field and water planting condition; The solution that has no controlled release and residual selenium; Therefore, a large amount of sodium selenites that use or sodium selenate might spread the perhaps residual selenium contamination risk that causes soil in original culture matrix in environment.Seek a kind ofly to let garlic both can efficiently accumulate organic selenium to have the environmental protection culturing method that to avoid environment being produced the selenium contamination risk extremely to be necessary.The present invention therefore.
Summary of the invention
The purpose of this invention is to provide a kind of method of cultivating the selenium-enriched garlic vegetable material, it is not long to have solved in the prior art in the garlic vegetable material selenium persistence, is easy in environment, be reduced and problem such as availability is limited.
In order to solve these problems of the prior art, technical scheme provided by the invention is following:
A kind of method of cultivating the selenium-enriched garlic vegetable material; Se content is at 3mg/kg～1500mg/kg in the said selenium-enriched garlic vegetable material; And the selenium so that the methylselenocysteinefrom form exists accounts for more than 80% of total Se content ratio; It is characterized in that said method is included in the process of growth of garlic adds selenium source in the garlic somatomedin; Use selenium source to regulate that Se content is 0.2～100mg/kg in the somatomedin, and add the step that somatomedin integral body or local environment pH value is adjusted to 7～8 alkaline matter simultaneously.
Preferably, the selenium source material is selected from selenite, selenate, selenium yeast, contains selenium ore or two or more any mixing in the said method.
Preferably, said method also is included in when adding selenium source, adds the selenium source adsorbent of the physical adsorption selenium source of effective dose.
Preferably, to be selected from selenium source material mass ratio be 1: 1 active carbon, clay mineral, cellulosic material to the said selenium source adsorbent of said method.
Preferably, said method alkaline matter is selected from white lime, sodium hydroxide or potassium hydroxide.
Preferably, said method also comprises when adding selenium source, adds the promotion plant selenium that selenium absorbs is absorbed promoter, and said selenium absorbs promoter and selects organic matter for use.
Preferably, in the said method selenium absorb promoter select for use with selenium source quality weight ratio be 10-100: 1 humic acid.
Preferably, in the said method selenium absorb promoter select for use with selenium source quality weight ratio be 10-100: 1 fulvic acid.
Described in the technical scheme of the present invention in the selenium-enriched garlic vegetable material Se content and account for more than 80% of total Se content ratio at 5mg/kg～1200mg/kg with the selenium that the methylselenocysteinefrom form exists.Preferred, Se content and accounts for more than 80% of total Se content ratio with the selenium that the methylselenocysteinefrom form exists at 5mg/kg～1000mg/kg in the said selenium-enriched garlic vegetable material.Most preferred, Se content and accounts for more than 80% of total Se content ratio with the selenium that the methylselenocysteinefrom form exists at 10mg/kg～1000mg/kg in the said selenium-enriched garlic vegetable material.
The producer ratio juris that the garlic of methylselenocysteinefrom is rich in the present invention is in the process of growth of garlic; In its somatomedin, add an amount of selenium source, selenium source adsorbent, selenium biologically active retention agent and selenium and absorb promoter; To reach stable and effective selenium source supply is provided in the somatomedin of garlic; And promote the garlic absorption and accumulation, realize that selenium effectively shifts to garlic from soil, avoids the excessive murder by poisoning risk to the garlic growing environment of selenium.
The present invention provides and utilizes garlic that the technical scheme that inorganic selenium is converted into methylselenocysteinefrom is following: in the process of growth of garlic, in its somatomedin, add a certain amount of selenium source and selenium source adsorbent; Make the Se content in the somatomedin reach 0.2～100mg/kg and realize selenium source material fixing in the garlic somatomedin, avoid running off; Add simultaneously can prevent that biological available state selenium is reduced in somatomedin and the alkaline matter that lost efficacy keeping the biologically active of selenium, described selenium biologically active retention agent is meant the organic bases or the inorganic base that can garlic somatomedin integral body or local modulation be become alkalescence (pH 7～8); Also in the somatomedin of garlic, add selenium in addition and absorb promoter, described selenium absorbs the material that promoter being meant can promote that plant absorbs selenium, and like humic acid or fulvic acid, the weight ratio of its addition and selenium source material is 10-100: 1.The vegetable material that is rich in methylselenocysteinefrom that the present invention obtains can be used as the organic selenium fill-in and in food, feed, adds application.
Wherein, the selenium source material is selenite, selenate, selenium yeast, contain selenium ore or a few person mixing with any ratio, and as preferably, said selenium source material is meant sodium selenite; Described selenium source adsorbent is meant the material that the selenium source material is had certain adsorption function, like active carbon, clay mineral, cellulose etc.; Described biologically active retention agent is meant hydroxide.As preferably, said biologically active retention agent is meant slaked lime, sodium hydroxide or potassium hydroxide.
Add selenium biologically active retention agent when in the somatomedin of garlic, adding selenium; With local flow improvement in the somatomedin or on every side is pH at 7～8 alkalescence environment; Can prevent effectively that available state selenium is reduced into the selenium of element state and can not be absorbed by plant; Make the effective selenium that adds to keep long biological effectiveness; Whole growth in garlic provides stable selenium source supply in period, at somatomedin selenium selenium concentration be to have solved under the condition of 0.2～100mg/kg in garlic the selenium problem of resistance, and finally make garlic reach total selenium accumulation effect of 3mg/kg～1500mg/kg.
In the somatomedin of garlic, add the selenium source adsorbent; The material that certain adsorption function can be arranged the selenium source material; Like active carbon, clay mineral, cellulose etc., can make the selenium source relative fixed in the somatomedin of garlic, avoid running off and to the environment build-up of pressure.
The selenium that in the somatomedin of garlic, adds absorbs promoter; Its humic acid, xanthohumic acid kind that can promote plant that selenium is absorbed is organic; Can promote garlic absorbing to selenium; Under the identical condition of other conditions, can the Se content of garlic be improved 10～25%, can realize the more good utilisation of the selenium of interpolation, avoid the loss of selenium to cause environmental hazard.
Resulting high selenium garlic can be used as human body and animal selenium source supplementation material preferably, after drying and crushing, is adding as selenium source in occasions such as food, feeds, is of value to humans and animals healthy selenium-enriched food, Se-enriched feedstuff to produce.
Compare prior art, the present invention has following beneficial effect:
It is big that method of the present invention has broken through in the prior art selenium usage amount; Be easy in environment, be reduced; Availability is low, possibly have defectives such as poisoning risk to environment, has realized the high selenium of garlic is cultivated; And taken into full account the tolerance limit of garlic, under the situation that it is not produced the selenium murder by poisoning, formed accumulating of organic selenium higher concentration selenium.
Embodiment:
Following examples are used to explain the present invention, but can not be used for limiting scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and unaccounted implementation condition is generally the condition in the normal experiment.
Embodiment 1:
Under the soil cultivation condition; Seedling phase of garlic with sodium selenite with mix with a certain amount of white lime again after the humic acid of the active carbon of equal quality, 10 times of quality mixes obtaining the mixture of pH 7～8, and it is applied in the soil of garlic growth, make Se content average out to 0.2mg/kg in the topsoil of its growth; During to results; Gather garlic and carry out the test of Se content, wherein total Se content is about 3mg/kg, and the availability of selenium is than exceeding about 10% under the normal condition.
Under the water planting condition, with sodium selenite and sodium selenate with any than mixing humic acid that the back adds 20 times of quality in culture matrix, regulate culture matrix pH regulator to 7～8; Making wherein, Se content on average remains on 0.2mg/kg; During to results, gather garlic and carry out the test of Se content, wherein total Se content is about 4mg/kg; Wherein, the availability of selenium is than exceeding about 15% under the normal condition.
Embodiment 2
Under the soil cultivation condition; Growth stage of garlic with sodium selenate with mix with a certain amount of potassium hydroxide again after the fulvic acid of the active carbon of equal quality, 30 times of quality mixes obtaining the mixture of pH 7～8, and it is applied in the soil of garlic growth, make Se content average out to 5mg/kg in the topsoil of its growth; During to results; Gather garlic and carry out the test of Se content, wherein total Se content is about 20mg/kg, and the availability of selenium is than exceeding about 15% under the normal condition.
Under the water planting condition, with sodium selenite and sodium selenate with any than mixing fulvic acid that the back adds 40 times of quality in culture matrix, regulate culture matrix pH regulator to 7～8; Making wherein, Se content on average remains on 5mg/kg; During to results, gather garlic and carry out the test of Se content, wherein total Se content is about 4mg/kg; Wherein, the availability of selenium is than exceeding about 20% under the normal condition.
Embodiment 3
Under the soil cultivation condition; The humic acid that will contain selenium ore and the active carbon of equal quality, 50 times of quality at the flower bud differentiation period of garlic with mix with a certain amount of white lime again to obtain the mixture of pH7～8 after fulvic acid mixes; And it is applied in the soil of garlic growth; Make Se content average out to 10mg/kg in the topsoil of its growth, during to results, gather garlic and carry out the test of Se content; Wherein total Se content is about 100mg/kg, and the availability of selenium is than exceeding about 15% under the normal condition.
Under the water planting condition, with sodium selenite and sodium selenate with any than the mixture that mixes fulvic acid that the back adds 60 times of quality and humic acid in culture matrix, adjusting culture matrix pH regulator to 7～8; Making wherein, Se content on average remains on 10mg/kg; During to results, gather garlic and carry out the test of Se content, wherein total Se content is about 150mg/kg; Wherein, the availability of selenium is than exceeding about 20% under the normal condition.
Embodiment 4
Under the soil cultivation condition; After the flower bud differentiation period of garlic will contain yeast selenium and the fulvic acid of the active carbon of equal quality, 80 times of quality mixes, mix with a certain amount of white lime again obtaining the mixture of pH 7～8, and it is applied in the soil of garlic growth, make Se content average out to 50mg/kg in the topsoil of its growth; During to results; Gather garlic and carry out the test of Se content, wherein total Se content is about 700mg/kg, and the availability of selenium is than exceeding about 15% under the normal condition.
Under the water planting condition, with sodium selenite and sodium selenate with any than mixing fulvic acid that the back adds 90 times of quality in culture matrix, regulate culture matrix pH regulator to 7～8; Making wherein, Se content on average remains on 50mg/kg; During to results, gather garlic and carry out the test of Se content, wherein total Se content is about 900mg/kg; Wherein, the availability of selenium is than exceeding about 25% under the normal condition.
Embodiment 5
Under the soil cultivation condition; After the flower bud differentiation period of garlic will contain yeast selenium and the fulvic acid of the active carbon of equal quality, 100 times of quality mixes, mix with a certain amount of white lime again obtaining the mixture of pH 7～8, and it is applied in the soil of garlic growth, make Se content average out to 100mg/kg in the topsoil of its growth; During to results; Gather garlic and carry out the test of Se content, wherein total Se content is about 1500mg/kg, and the availability of selenium is than exceeding about 15% under the normal condition.
Under the water planting condition, with sodium selenite and sodium selenate with any than mixing fulvic acid that the back adds 100 times of quality in culture matrix, regulate culture matrix pH regulator to 7～8; Making wherein, Se content on average remains on 100mg/kg; During to results, gather garlic and carry out the test of Se content, wherein total Se content is about 1700mg/kg; Wherein, the availability of selenium is than exceeding about 20% under the normal condition.
Above-mentioned instance only is explanation technical conceive of the present invention and characteristics, and its purpose is to let the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalent transformations that spirit is done according to the present invention or modification all should be encompassed within protection scope of the present invention.
Claims (8)
1. method of cultivating the selenium-enriched garlic vegetable material; Se content is at 3mg/kg ~ 1500mg/kg in the said selenium-enriched garlic vegetable material; And the selenium so that the methylselenocysteinefrom form exists accounts for more than 80% of total Se content ratio; It is characterized in that said method is included in the process of growth of garlic adds selenium source in the garlic somatomedin; Use selenium source to regulate that Se content is 0.2 ~ 100 mg/kg in the somatomedin, and add the step that somatomedin integral body or local environment pH value is adjusted to 7 ~ 8 alkaline matter simultaneously.
2. method according to claim 1 is characterized in that the selenium source material is selected from selenite, selenate, selenium yeast in the said method, contains selenium ore or two or more any mixing.
3. method according to claim 1 is characterized in that said method also is included in when adding selenium source, adds the selenium source adsorbent of the physical adsorption selenium source of effective dose.
4. method according to claim 3, it is characterized in that the said selenium source adsorbent of said method be selected from the selenium source material mass than being the active carbon of 1:1, clay mineral, cellulosic material.
5. method according to claim 3 is characterized in that said method alkaline matter is selected from white lime, sodium hydroxide or potassium hydroxide.
6. method according to claim 3 is characterized in that said method also comprises when adding selenium source, adds to promote that plant absorbs promoter to the selenium that selenium absorbs, and said selenium absorbs promoter and selects organic matter for use.
7. method according to claim 6, it is characterized in that in the said method selenium absorb promoter select for use with selenium source quality weight ratio be the humic acid of 10-100:1.
8. method according to claim 6, it is characterized in that in the said method selenium absorb promoter select for use with selenium source quality weight ratio be the fulvic acid of 10-100:1.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102771295A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Method for improving selenium fertilizer absorption efficiency of tea trees |
| CN102792840A (en) * | 2012-08-15 | 2012-11-28 | 苏州硒谷科技有限公司 | Organic selenium-rich dendrobium candidum and cultivation method thereof |
| CN103004567A (en) * | 2012-12-28 | 2013-04-03 | 李新荣 | Selenium-enriched onions and preparation method thereof |
| CN103891510A (en) * | 2014-04-01 | 2014-07-02 | 张大跃 | Garlic sprout seed cultivation method |
| CN104919938A (en) * | 2015-06-30 | 2015-09-23 | 山东省慧福康富硒生物科技有限公司 | Planting method of selenium-rich single-clove garlic |
| CN104938190A (en) * | 2015-06-30 | 2015-09-30 | 山东省慧福康富硒生物科技有限公司 | Selenium-enriched wheat planting method |
| CN104996130A (en) * | 2015-06-30 | 2015-10-28 | 山东省慧福康富硒生物科技有限公司 | Planting method for selenium-rich garlic |
| CN105123375A (en) * | 2015-07-20 | 2015-12-09 | 田东县八闽农业综合开发有限公司 | Cultivation method for selenium-rich mangos |
| CN106613228A (en) * | 2016-12-21 | 2017-05-10 | 王春晓 | Garlic selenium enrichment method |
| CN115989779A (en) * | 2023-03-13 | 2023-04-21 | 辽宁大学 | Organic quantitative selenium-rich cultivation method for northeast single garlic |
| CN116268323A (en) * | 2022-04-11 | 2023-06-23 | 王毅 | Selenium-enriched garlic product and production method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102771295A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Method for improving selenium fertilizer absorption efficiency of tea trees |
| CN102792840A (en) * | 2012-08-15 | 2012-11-28 | 苏州硒谷科技有限公司 | Organic selenium-rich dendrobium candidum and cultivation method thereof |
| CN103004567A (en) * | 2012-12-28 | 2013-04-03 | 李新荣 | Selenium-enriched onions and preparation method thereof |
| CN103004567B (en) * | 2012-12-28 | 2014-07-23 | 李新荣 | Selenium-enriched onions and preparation method thereof |
| CN103891510A (en) * | 2014-04-01 | 2014-07-02 | 张大跃 | Garlic sprout seed cultivation method |
| CN104919938A (en) * | 2015-06-30 | 2015-09-23 | 山东省慧福康富硒生物科技有限公司 | Planting method of selenium-rich single-clove garlic |
| CN104938190A (en) * | 2015-06-30 | 2015-09-30 | 山东省慧福康富硒生物科技有限公司 | Selenium-enriched wheat planting method |
| CN104996130A (en) * | 2015-06-30 | 2015-10-28 | 山东省慧福康富硒生物科技有限公司 | Planting method for selenium-rich garlic |
| CN105123375A (en) * | 2015-07-20 | 2015-12-09 | 田东县八闽农业综合开发有限公司 | Cultivation method for selenium-rich mangos |
| CN106613228A (en) * | 2016-12-21 | 2017-05-10 | 王春晓 | Garlic selenium enrichment method |
| CN116268323A (en) * | 2022-04-11 | 2023-06-23 | 王毅 | Selenium-enriched garlic product and production method thereof |
| CN115989779A (en) * | 2023-03-13 | 2023-04-21 | 辽宁大学 | Organic quantitative selenium-rich cultivation method for northeast single garlic |
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Application publication date: 20120627 |