CN102511748A - Selenium-enriched cardamine hupingshanesis plant material as well as cultivation method and application for same - Google Patents

Selenium-enriched cardamine hupingshanesis plant material as well as cultivation method and application for same Download PDF

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CN102511748A
CN102511748A CN2011104262688A CN201110426268A CN102511748A CN 102511748 A CN102511748 A CN 102511748A CN 2011104262688 A CN2011104262688 A CN 2011104262688A CN 201110426268 A CN201110426268 A CN 201110426268A CN 102511748 A CN102511748 A CN 102511748A
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selenium
content
hairy bittercress
tall bottle
spout hairy
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李飞
李伟
鲁钰良
袁林喜
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Suzhou Setek Co Ltd
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Suzhou Setek Co Ltd
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Priority to PCT/CN2012/070479 priority patent/WO2013091291A1/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
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  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
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  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a selenium-enriched cardamine hupingshanesis plant material and a cultivation method for the same, wherein selenium in the plant material exists in the form of selenocysteine, and the content of the selenium in the form of selenocysteine in the plant material is 0.2-20000 mg/kg and occupies more than 90% of the total content ratio of selenium. The selenium-enriched cardamine hupingshanesis plant material is cultivated according to a conventional method, wherein the conventional method comprises the following steps of: adding a selenium source in the growth medium of cardamine hupingshanesis during the growth cycle of the cardamine hupingshanesis; adjusting the content of selenium in the growth medium to achieve 0.1-1000 mg/kg; and adding alkaline substances simultaneously to keep the pH value of the overall or local environment of the growth medium in a range from 7 to 8. The material can be used as good selenium source supplementary substance for human body and animals, and directly eaten or used as selenium-enriched food or selenium-enriched feed beneficial to the health of human and animals.

Description

Rich selenium tall bottle with spout Hairy Bittercress vegetable material and breeding method and application
Technical field
The present invention relates to a kind of selenium-enriched plant material, particularly relate to a kind of breeding method and application of being rich in tall bottle with spout Hairy Bittercress vegetable material and this material of selenocysteine, belong to the cultivation technique field.
Technical background
Selenium is trace element essential in the humans and animals body, is to constitute the nearly activated centre of 23 kinds of functional proteins such as glutathione peroxidase (GSH-Px), selenoprotein P, have anticancer, anti-oxidant, improve effect such as immunity.To the greatest extent China has 72% area day selenium intake significantly to be lower than the selenium daily intaking amount that The World Health Organization (WHO) is recommended approximately, has caused serious human body and animal health problem.
Selenium totally is divided into inorganic selenium and organic selenium two big classes: the former comprises selenate, selenite etc., and inorganic selenium assimilation effect for humans and animals is poor, and risk of toxicity is big, and improper use very easily causes selenosis; The latter mainly is meant the selenium with the biological organic molecule combining form, and organic selenium absorptivity is high concerning humans and animals, and risk of toxicity is little, is more suitable for mending the requirement of selenium.The further segmentation of organic selenium mainly comprises selenomethionine, selenocysteine, also is included in all seldom selenium polysaccharides, seleno nucleic acid etc. of content in the animals and plants.And also there is bigger difference in the above two metabolism in the humans and animals body, and main non-specific binding was in proteosome after selenomethionine got into body; Storage might not be brought into play biological function as selenium; Selenocysteine then directly gets in the metabolic pathway of synthesizing of selenoprotein, is its biology merit play a role (progress of the organic selenium of yeast, Chen Min, Li Feng; Feed research, 2010).
At present, the exploitation of rich selenium product and associated materials increases gradually, and method is also varied, but does not all also relate to the rich selenium material that specific selenium form is rich in directive breeding.Like application number is the production method that 200810017759.5 one Chinese patent application discloses a kind of organic selenium; This method utilizes the growth of plant that inorganic selenium is converted into organic selenium; After the seed of plant soaked with the inorganic salts sodium selenite solution again through germination, plantation; Growth through plant is converted into organic selenium with inorganic selenium, just becomes organic selenium product through handling then.Its kind is limited to common mung bean, cowpea, tomato, cucumber, and these kinds are limited and form is uncertain to the accumulation of selenium.
The tall bottle with spout Hairy Bittercress is a kind of new variety of plant of finding bestowing favour, and it has the good property accumulated to selenium.Thesis for the doctorate " biological characteristics of the Hairy Bittercress of bestowing favour and blade selenium-containing compound research " (Xiang Tianyong, Agricultural University Of Hunan, 2006) has carried out comparatively detailed report to the biological characteristics of tall bottle with spout Hairy Bittercress and to the absorption and accumulation metabolic characteristic of selenium.And the experiment that utilizes selenium concentration the tall bottle with spout Hairy Bittercress to be carried out floating cultivation for the sodium selenite solution of 10-50mg/L disclosed; Obtained the Hairy Bittercress tissue of Se content 300-1000mg/kg, and the main selenium form that analysis draws wherein is water miscible micromolecular compound.But; Its research only limits to add with water planting the selenium absorption and the characteristic of accumulation of the means exploration tall bottle with spout Hairy Bittercress of sodium selenite; Simple sodium selenite is reduced in the water body medium, also discloses selenium in this paper and has got into the phenomenon that is reduced into red simple substance selenium in the tall bottle with spout Hairy Bittercress tissue very soon, is unfavorable for the further organic transformation of inorganic selenium in plant; In addition, this piece paper does not relate to the directive breeding of its form yet.
Organic selenium in the plant all is that the form with selenomethionine exists usually, for example cereal crops such as wheat, corn (Li Chunxi, the precious treasure of wormwood artemisia etc., the progress of selenium in the wheat growth growth course, Anhui agricultural sciences, 2007) after selenium nutrition-intensified; The plant that also has minority section to belong to can enrichment be more suitable for directly adding to selenocysteine such as liliaceous some crop (garlic, lily etc.) (Du Yuxiao in the metabolic pathway; Li Yanan etc.; The plant seleno is thanked the progress of accumulation and relevant enzyme, tropical and subtropical zone Botany Gazette, 2007).But garlic etc. be because its smell can influence it usually adds as food or feed, extremely are necessary so develop the vegetable matter of a kind ofly accumulating the concentration height, being rich in selenocysteine.The present invention therefore.
Summary of the invention
The purpose of this invention is to provide a kind of tall bottle with spout Hairy Bittercress vegetable material that is rich in selenocysteine; The content of the selenium that exists with the selenocysteine form in this vegetable material is 0.18mg/kg~18000mg/kg; It is (to be that total Se content is 0.2mg/kg~20000mg/kg), a kind of new organic selenium vegetable material of accumulating high-load is provided more than 90% that the selenium of this form accounts for total selenium ratio.
In order to solve these problems of the prior art, technical scheme provided by the invention is following:
A kind of rich selenium tall bottle with spout Hairy Bittercress vegetable material; It is characterized in that selenium exists with the selenocysteine form in the said vegetable material; And the content of selenium in said vegetable material with the selenocysteine form is 0.2mg/kg~20000mg/kg, accounts for more than 90% of total Se content ratio.
Preferably, said vegetable material is 0.2mg/kg~1.0mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
Preferably, said vegetable material is 200mg/kg~2000mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
Preferably, said vegetable material is 2000mg/kg~12000mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
Another object of the present invention is to provide a kind of breeding method of rich selenium tall bottle with spout Hairy Bittercress vegetable material; Said rich selenium tall bottle with spout Hairy Bittercress vegetable material is cultivated according to conventional method; It is characterized in that said method also comprises in the somatomedin of the inherent tall bottle with spout Hairy Bittercress of growth cycle of tall bottle with spout Hairy Bittercress adds selenium source; The Se content of regulating in the somatomedin reaches 0.1~1000mg/kg, and adds alkaline matter simultaneously and make the integral body of somatomedin or the step of pH value in 7~8 scopes of local environment.
Preferably, selenium source described in the said method is selected from one or more any mixing of sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen.
Preferably, alkaline matter described in the said method is selected from one or more any mixing in white lime, NaOH, the potassium hydroxide.
Preferably, when the adding selenium source was regulated in the said method, also adding was 10-100 with the weight ratio of selenium source material: 1 humic acid.
Preferably, when the adding selenium source was regulated in the said method, also adding was 10-100 with the weight ratio of selenium source material: 1 fulvic acid.
Another purpose of the present invention is to provide a kind of described rich selenium tall bottle with spout Hairy Bittercress vegetable material as the application in organic selenium-rich food, the feed.
The content of the selenium that exists with the selenocysteine form in the rich selenium tall bottle with spout Hairy Bittercress vegetable material among the present invention is 0.2mg/kg~20000mg/kg, accounts for more than 90% of total Se content ratio.Preferably, the content of the selenium that exists with the selenocysteine form is 0.2mg/kg~12000mg/kg, accounts for more than 90% of total Se content ratio.Preferred; The content of the selenium that exists with the selenocysteine form is 200mg/kg~2000mg/kg; The content that accounts for the selenium that exists more than 90% or with the selenocysteine form of total Se content ratio is 2000mg/kg~12000mg/kg, accounts for more than 90% of total Se content ratio.
When the present invention cultivated rich selenium tall bottle with spout Hairy Bittercress vegetable material, need in the growth course of tall bottle with spout Hairy Bittercress, use selenium source to regulate its Se content was 0.1~1000mg/kg, and added alkaline matter simultaneously somatomedin integral body or local pH are adjusted to 7~8.Said selenium source be adjusted to wherein one or more of sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen with any than mixing.Said alkaline matter be wherein one or more of white lime, NaOH, potassium hydroxide with any than mixing.When adding selenium source, preferably adding is 10-100 with the weight ratio of selenium source material: 1 humic acid.Preferred employing is 10-100 with the weight ratio of selenium source material: 1 fulvic acid.The rich selenium tall bottle with spout Hairy Bittercress vegetable material that obtains can be used as organic selenium fill-in and in food, feed, adds application.
The principle that the present invention cultivates the tall bottle with spout Hairy Bittercress vegetable material that is rich in selenocysteine is: in the growth course of tall bottle with spout Hairy Bittercress; In its somatomedin, add an amount of selenium source, alkaline matter; Can also add selenium to it and absorb promoter; Reaching provides stable and effective selenium source supply in the somatomedin of tall bottle with spout Hairy Bittercress, and is beneficial to tall bottle with spout Hairy Bittercress absorption and accumulation.The tall bottle with spout Hairy Bittercress is owing to its unique biological characteristic, and the selenium in the plant all exists with the form of selenocysteine more than 90%.
The present invention utilizes the tall bottle with spout Hairy Bittercress that the method that inorganic selenium is converted into organic selenium is following: in the growth course of tall bottle with spout Hairy Bittercress, in its somatomedin, add a certain amount of selenium source; Make the Se content in the somatomedin reach 0.1~1000mg/kg; And add the alkaline matter to prevent to be reduced during biological available state selenium is under field condition and to lose efficacy simultaneously; Said alkaline matter is meant the organic bases or the inorganic base that can tall bottle with spout Hairy Bittercress somatomedin integral body or local modulation be become alkalescence (pH 7~8), is selenium biologically active retention agent.
As preferably, said alkaline matter is meant hydroxide.As preferably, said alkaline matter is meant calcium hydroxide, NaOH or potassium hydroxide.
Technical scheme provided by the invention can also be included in and add selenium in the somatomedin of tall bottle with spout Hairy Bittercress and absorb promoter; Its consumption is that described selenium absorbs the organic matter that promoter being meant can promote that plant absorbs selenium, and the weight ratio of its addition and selenium source material is 10-100: 1.As preferably, described selenium absorbs promoter and is meant humic acid.As preferably, described selenium absorbs promoter and is meant fulvic acid.
Described growth cycle process preferably adopts before seedling phase, the bolting and peduncle-growing period for rapeseed.
Compare prior art, the present invention has following beneficial effect:
Add selenium biologically active retention agent when in the somatomedin of tall bottle with spout Hairy Bittercress, adding selenium; With local flow improvement in the somatomedin or on every side is pH at 7~8 alkalescence environment; Can prevent effectively that available state selenium is reduced into the selenium of element state and can not be absorbed by plant; Make the effective selenium that adds to keep long biological effectiveness; Whole growth at the tall bottle with spout Hairy Bittercress provides stable selenium source supply in period; Be finally to make the tall bottle with spout Hairy Bittercress reach total selenium accumulation effect of 0.2mg/kg~20000mg/kg under the condition of 0.1~1000mg/kg at somatomedin selenium selenium concentration, and realize in the tall bottle with spout Hairy Bittercress that about 90% selenium form exists with the form of the selenocysteine that can directly get into human body and animal absorption metabolism.
The selenium that in the somatomedin of tall bottle with spout Hairy Bittercress, adds absorbs promoter; Its humic acid, xanthohumic acid kind that can promote plant that selenium is absorbed is organic; Can promote tall bottle with spout Hairy Bittercress absorbing to selenium; Under the identical condition of other conditions, can the Se content of tall bottle with spout Hairy Bittercress be improved 10~25%, can realize the more good utilisation of the selenium of interpolation, avoid the loss of selenium to cause environmental hazard.
Resulting high selenium material can be used as human body and animal selenium source supplementation material preferably; Directly eat or after drying and crushing, add as selenium source, be of value to humans and animals healthy selenium-enriched food, Se-enriched feedstuff to produce in occasions such as food, feeds.
The specific embodiment:
Following examples are used to explain the present invention, but can not be used for limiting scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and unaccounted implementation condition is generally the condition in the normal experiment.
Embodiment 1
Under the soil cultivation condition, in seedling phase of tall bottle with spout Hairy Bittercress sodium selenite is mixed with a certain amount of white lime obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 0.1mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 0.2mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, be adjusted to 7~8, and join in the culture matrix after sodium selenite and sodium selenate mixed with any coin at the culture matrix pH of tall bottle with spout Hairy Bittercress; Make the wherein average 0.1mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 0.5mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenite is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 10; To obtain the mixture of pH 7; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 0.1mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 0.22mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, be adjusted to 7~8, and sodium selenite and sodium selenate mixed with any coin to add behind the cloud with its mass ratio be that 10: 1 humic acid is inserted in the fluid nutrient medium again at the culture matrix pH of tall bottle with spout Hairy Bittercress; Make the wherein average 0.1mg/kg of Se content; And to when results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 0.6mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 2
Under the soil cultivation condition, before the bolting of tall bottle with spout Hairy Bittercress, sodium selenate is mixed with a certain amount of NaOH obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 0.5mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 1.0mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 7.5, and joins in the culture matrix after sodium selenite, sodium selenate, seleno-amino acids mixed with any coin; Make the wherein average 0.5mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 2.5mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of NaOH in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 20; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 0.5mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 1.2mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, be adjusted to 7~8 at the culture matrix pH of tall bottle with spout Hairy Bittercress, and with sodium selenite and sodium selenate being that 20: 1 humic acid is inserted in the fluid nutrient medium than adding again behind the mixing arbitrarily with its mass ratio; Make the wherein average 0.5mg/kg of Se content; And to when results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 3.0mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 3
Under the soil cultivation condition, in seedling phase of tall bottle with spout Hairy Bittercress sodium selenite is mixed with a certain amount of potassium hydroxide obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 5mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 20mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 8, and sodium selenite, sodium selenate, seleno-amino acids are joined in the culture matrix after mixing with any; Make the wherein average 5mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 50mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenite is mixed with a certain amount of potassium hydroxide in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of fulvic acid with 1: 10; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 5mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 25mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, be adjusted to 7~8 at the culture matrix pH of tall bottle with spout Hairy Bittercress, and with sodium selenite and sodium selenate being that 10: 1 fulvic acid is inserted in the fluid nutrient medium than adding again behind the mixing arbitrarily with its mass ratio; Make the wherein average 5mg/kg of Se content; And to when results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 70mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 4
Under the soil cultivation condition, in seedling phase of tall bottle with spout Hairy Bittercress sodium selenate is mixed with a certain amount of white lime obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 10mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 300mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 7.5, and sodium selenite, sodium selenate, seleno-amino acids are joined in the culture matrix after mixing with any; Make the wherein average 10mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 500mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 10; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 10mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 350mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, be adjusted to 7~8 at the culture matrix pH of tall bottle with spout Hairy Bittercress, and with sodium selenite and sodium selenate being that 10: 1 humic acid is inserted in the fluid nutrient medium than adding again behind the mixing arbitrarily with its mass ratio; Make the wherein average 10mg/kg of Se content; And to when results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 700mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 5
Under the soil cultivation condition, in seedling phase of tall bottle with spout Hairy Bittercress Se-enriched yeast is mixed with a certain amount of white lime obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 50mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 1000mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 8, and yeast is milled to 200 orders joins in the culture matrix later on; Make the wherein average 50mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 1500mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 10; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 50mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 1200mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 8, and yeast is milled to 200 orders and humic acid joins in the culture matrix after with 1: 10 mixed; Make the wherein average 50mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 1800mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 6
Under the soil cultivation condition, in seedling phase of tall bottle with spout Hairy Bittercress Se-enriched yeast is mixed with a certain amount of white lime obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 100mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 2000mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 7, and selenide of carragheen is milled to 200 orders joins in the culture matrix later on; Make the wherein average 100mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 2500mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 10; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 100mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 2200mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the water planting condition, before the bolting of tall bottle with spout Hairy Bittercress, culture matrix pH is adjusted to 8, and selenide of carragheen is milled to 200 orders and humic acid joins in the culture matrix after with 1: 10 mixed; Make the wherein average 100mg/kg of Se content; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 2800mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 7
Under the soil cultivation condition, at the peduncle-growing period for rapeseed of tall bottle with spout Hairy Bittercress Se-enriched yeast is mixed with a certain amount of white lime obtaining the mixture of pH 7~8, and it is applied in the soil of tall bottle with spout Hairy Bittercress growth; Make Se content average out to 250mg/kg in the topsoil of its growth; During to results, gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content, wherein total Se content is about 5000mg/kg; Wherein, the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of humic acid with 1: 30; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 250mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 6000mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 8
Under the soil cultivation condition; Peduncle-growing period for rapeseed at the tall bottle with spout Hairy Bittercress is spared sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen to obtain the mixture of pH7~8 with a certain amount of white lime than mixing the back with any; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 500mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 10000mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of fulvic acid with 1: 50; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 500mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 12500mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 9
Under the soil cultivation condition; Peduncle-growing period for rapeseed at the tall bottle with spout Hairy Bittercress is spared sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen to obtain the mixture of pH7~8 with a certain amount of white lime than mixing the back with any; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 750mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 15000mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of fulvic acid with 1: 80; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 750mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 17500mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Embodiment 10
Under the soil cultivation condition; Peduncle-growing period for rapeseed at the tall bottle with spout Hairy Bittercress is spared sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen to obtain the mixture of pH7~8 with a certain amount of white lime than mixing the back with any; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 1000mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 20000mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Under the soil cultivation condition, sodium selenate is mixed with a certain amount of white lime in the seedling phase of tall bottle with spout Hairy Bittercress, and with the ratio mixing of fulvic acid with 1: 100; To obtain the mixture of pH 7~8; And it is applied in the soil of tall bottle with spout Hairy Bittercress growth, make Se content average out to 1000mg/kg in the topsoil of its growth, when gathering in the crops; Gather tall bottle with spout Hairy Bittercress plant and carry out the test of Se content; Wherein total Se content is about 22000mg/kg, wherein, and the selenium accounting about 90% that exists with the selenomethionine form.
Above-mentioned instance only is explanation technical conceive of the present invention and characteristics, and its purpose is to let the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalent transformations that spirit is done according to the present invention or modification all should be encompassed within protection scope of the present invention.

Claims (10)

1. rich selenium tall bottle with spout Hairy Bittercress vegetable material; It is characterized in that selenium exists with the selenocysteine form in the said vegetable material; And the content of selenium in said vegetable material with the selenocysteine form is 0.2 mg/kg ~ 20000 mg/kg, accounts for more than 90% of total Se content ratio.
2. rich selenium tall bottle with spout Hairy Bittercress vegetable material according to claim 1; It is characterized in that said vegetable material is 0.2 mg/kg ~ 1.0 mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
3. rich selenium tall bottle with spout Hairy Bittercress vegetable material according to claim 1; It is characterized in that said vegetable material is 200 mg/kg ~ 2000 mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
4. rich selenium tall bottle with spout Hairy Bittercress vegetable material according to claim 1; It is characterized in that said vegetable material is 2000 mg/kg ~ 12000 mg/kg with the content of selenium in said vegetable material that the selenocysteine form exists, and accounts for more than 90% of total Se content ratio.
5. the breeding method of any described rich selenium tall bottle with spout Hairy Bittercress vegetable material of claim 1~4; Said rich selenium tall bottle with spout Hairy Bittercress vegetable material is cultivated according to conventional method; It is characterized in that said method also comprises in the somatomedin of the inherent tall bottle with spout Hairy Bittercress of growth cycle of tall bottle with spout Hairy Bittercress adds selenium source; The Se content of regulating in the somatomedin reaches 0.1 ~ 1000 mg/kg, and adds alkaline matter simultaneously and make the integral body of somatomedin or the step of pH value in 7 ~ 8 scopes of local environment.
6. method according to claim 5 is characterized in that selenium source described in the said method is selected from one or more any mixing of sodium selenite, sodium selenate, seleno-amino acids, Se-enriched yeast selenide of carragheen.
7. method according to claim 5 is characterized in that alkaline matter described in the said method is selected from one or more any mixing in white lime, NaOH, the potassium hydroxide.
8. method according to claim 5, when it is characterized in that the adding selenium source is regulated in the said method, also adding is the humic acid of 10-100:1 with the weight ratio of selenium source material.
9. method according to claim 5, when it is characterized in that the adding selenium source is regulated in the said method, also adding is the fulvic acid of 10-100:1 with the weight ratio of selenium source material.
Among the claim 1-4 each described rich selenium tall bottle with spout Hairy Bittercress vegetable material as the application in organic selenium-rich food, the feed.
CN2011104262688A 2011-12-19 2011-12-19 Selenium-enriched cardamine hupingshanesis plant material as well as cultivation method and application for same Pending CN102511748A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103053805A (en) * 2012-11-30 2013-04-24 湖北盛硒生物科技有限公司 Selenium-rich feed additive and preparation method thereof
CN105685265A (en) * 2016-01-20 2016-06-22 恩施州地壹嘉知识产权服务有限公司 Selenium-rich cardamine hupingshanensis-camellia oil
CN106717995A (en) * 2017-01-19 2017-05-31 恩施土家族苗族自治州农业科学院 A kind of method that use selenium-rich nutritive block plants poly- selenium Hairy Bittercress
CN110616122A (en) * 2019-10-28 2019-12-27 陕西理工大学 Cardamine hupingshanensis selenium-rich yellow wine and production method thereof
CN110973637A (en) * 2019-10-30 2020-04-10 安徽中谷生物科技有限公司 Preparation method of plant micromolecular organic selenium
CN111345202A (en) * 2018-12-23 2020-06-30 陕西安康燚甸园聚硒植物科技开发有限公司 Greenhouse cultivation method for cardamine hupingshanensis
CN112868941A (en) * 2021-02-26 2021-06-01 海南晨海水产有限公司 Selenium-rich feed suitable for feeding siganus oramin and preparation method
CN115363198A (en) * 2021-05-17 2022-11-22 盐创(上海)科技有限公司 Preparation method of selenium-rich edible salt

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
向天勇: "恩施碎米荠的生物学特性及叶片含硒化合物研究", 《中国优秀博硕士学位论文全文数据库(博基础科学辑)》 *
张志明等: "腐植酸有机硒肥与食品安全及食疗健康", 《腐植酸》 *
赵春梅等: "植物富硒规律的研究进展", 《热带农业科学》 *
雷红灵等: "外源硒对恩施碎米荠叶片有效成分的影响", 《湖北农业科学》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103053805A (en) * 2012-11-30 2013-04-24 湖北盛硒生物科技有限公司 Selenium-rich feed additive and preparation method thereof
CN103053805B (en) * 2012-11-30 2014-04-16 湖北盛硒生物科技有限公司 Selenium-rich feed additive and preparation method thereof
CN105685265A (en) * 2016-01-20 2016-06-22 恩施州地壹嘉知识产权服务有限公司 Selenium-rich cardamine hupingshanensis-camellia oil
CN106717995A (en) * 2017-01-19 2017-05-31 恩施土家族苗族自治州农业科学院 A kind of method that use selenium-rich nutritive block plants poly- selenium Hairy Bittercress
CN111345202A (en) * 2018-12-23 2020-06-30 陕西安康燚甸园聚硒植物科技开发有限公司 Greenhouse cultivation method for cardamine hupingshanensis
CN110616122A (en) * 2019-10-28 2019-12-27 陕西理工大学 Cardamine hupingshanensis selenium-rich yellow wine and production method thereof
CN110973637A (en) * 2019-10-30 2020-04-10 安徽中谷生物科技有限公司 Preparation method of plant micromolecular organic selenium
CN112868941A (en) * 2021-02-26 2021-06-01 海南晨海水产有限公司 Selenium-rich feed suitable for feeding siganus oramin and preparation method
CN115363198A (en) * 2021-05-17 2022-11-22 盐创(上海)科技有限公司 Preparation method of selenium-rich edible salt
CN115363198B (en) * 2021-05-17 2024-02-13 盐创(上海)科技有限公司 Preparation method of selenium-enriched edible salt

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