CN1289525C - T2r,一类味觉受体 - Google Patents

T2r,一类味觉受体 Download PDF

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Publication number
CN1289525C
CN1289525C CNB00815435XA CN00815435A CN1289525C CN 1289525 C CN1289525 C CN 1289525C CN B00815435X A CNB00815435X A CN B00815435XA CN 00815435 A CN00815435 A CN 00815435A CN 1289525 C CN1289525 C CN 1289525C
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China
Prior art keywords
seq
taste
cells
protein
amino acid
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Expired - Fee Related
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CNB00815435XA
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English (en)
Chinese (zh)
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CN1387536A (zh
Inventor
C·S·朱克
J·E·阿德莱尔
N·里巴
K·米勒
M·胡恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
US Department of Health and Human Services
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University of California San Diego UCSD
US Department of Health and Human Services
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Priority claimed from US09/393,634 external-priority patent/US6558910B2/en
Application filed by University of California San Diego UCSD, US Department of Health and Human Services filed Critical University of California San Diego UCSD
Publication of CN1387536A publication Critical patent/CN1387536A/zh
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Publication of CN1289525C publication Critical patent/CN1289525C/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CNB00815435XA 1999-09-10 2000-09-08 T2r,一类味觉受体 Expired - Fee Related CN1289525C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/393,634 1999-09-10
US09/393,634 US6558910B2 (en) 1999-09-10 1999-09-10 SF, a novel family of taste receptors
US09/510,332 2000-02-22
US09/510,332 US7244584B2 (en) 1999-09-10 2000-02-22 T2R, a novel family of taste receptors

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CNA2006101598520A Division CN101058605A (zh) 1999-09-10 2000-09-08 T2r，一类味觉受体

Publications (2)

Publication Number Publication Date
CN1387536A CN1387536A (zh) 2002-12-25
CN1289525C true CN1289525C (zh) 2006-12-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CNB00815435XA Expired - Fee Related CN1289525C (zh) 1999-09-10 2000-09-08 T2r,一类味觉受体

Country Status (10)

Country Link
US (8) US7465550B2 (https=)
EP (1) EP1214343A2 (https=)
JP (1) JP4339542B2 (https=)
CN (1) CN1289525C (https=)
AU (2) AU773600B2 (https=)
CA (1) CA2384777A1 (https=)
IL (1) IL148593A0 (https=)
MX (1) MXPA02002538A (https=)
NO (1) NO20021164L (https=)
WO (1) WO2001018050A2 (https=)

Families Citing this family (43)

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Publication number Priority date Publication date Assignee Title
EP1214343A2 (en) 1999-09-10 2002-06-19 The Regents Of The University Of California T2r taste receptor family
US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
TW201006846A (en) * 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
AU5125801A (en) 2000-04-07 2001-10-23 Senomyx Inc T2r taste receptors and genes encoding same
TW201022287A (en) 2001-01-03 2010-06-16 Senomyx Inc T1R taste receptors and genes encoding same
US20080244761A1 (en) 2001-03-07 2008-10-02 Senomyx, Inc. T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US7368285B2 (en) 2001-03-07 2008-05-06 Senomyx, Inc. Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same
US7301009B2 (en) 2001-06-26 2007-11-27 Senomyx, Inc. Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli
US7883856B2 (en) 2001-04-05 2011-02-08 Senomyx Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US8030008B2 (en) 2001-04-05 2011-10-04 Senomyx, Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
EP2293067B1 (en) 2001-06-26 2016-04-20 Senomyx, Inc. T1R hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
DK2327985T3 (en) 2001-06-26 2016-09-05 Senomyx Inc H1 Oligomeric T1R Taste Receptors and Cell Lines Expressing the Receptors, and Their Use to Identify Taste Compounds
CN1610830A (zh) * 2001-07-10 2005-04-27 塞诺米克斯公司 特异性t2r味觉受体在鉴定阻断苦味觉的化合物中的应用
AU2002326429B2 (en) * 2001-07-20 2007-09-20 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Phenylthiocarbamide (PTC) taste receptor
WO2003031604A1 (en) * 2001-10-12 2003-04-17 The Regents Of The University Of California Gastrointestinal chemosensory receptors
IL165669A0 (en) 2002-07-29 2006-01-15 Senomyx Inc Identification of a novel bitter taste receptor t2r76
EP2272957A3 (en) * 2002-09-25 2011-08-03 Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke Bitter taste receptors
AU2003286069A1 (en) 2002-12-18 2004-07-09 Givaudan Sa Chimeric alpha q-gustducin g-proteins
US7579453B2 (en) 2003-06-19 2009-08-25 The United States Of America As Represented By The Secretary Of The Dapartment Of Health And Human Services Variants of human taste receptor genes
WO2006053771A2 (en) 2004-11-18 2006-05-26 Deutsches Institut Für Ernährungsforschung Potsdam-Rehbrücke Stiftung Des Öffentlichen Rechts Agonists of bitter taste receptors and uses thereof
JP2008537473A (ja) 2005-02-08 2008-09-18 セノミクス・インコーポレーテッド アセトアミノフェン、ラニチジン、ストリキニーネおよびデナトニウムのｐヒトＴ２Ｒ受容体、ならびにヒト苦味調節剤を同定するための関連アッセイ
JPWO2007074937A1 (ja) 2005-12-27 2009-06-04 学校法人日本大学 亜鉛欠乏性味覚異常検査法
US20110097741A1 (en) * 2006-04-20 2011-04-28 Jay Patrick Slack Partial t1r2 nucleic acid sequence, receptor protein and its use in screening assays
WO2007121604A2 (en) 2006-04-20 2007-11-01 Givaudan Sa Method relating to sweetness enhancement
JP2009533065A (ja) 2006-04-20 2009-09-17 ジボダン エス エー 味覚物質同定のための機能的方法
EP2064226A4 (en) * 2006-09-05 2010-03-31 Senomyx Inc NOVEL HUMAN T2R HT2R50 RECEPTOR HAPLOTYPE AND USE THEREOF IN ANALYZES TO IDENTIFY HUMAN MODULATORS OF BITTER TASTE
US8039233B2 (en) 2007-01-18 2011-10-18 Givaudan S.A. Nucleic acid sequences and their use in methods to identify umami modulatiors
EP2137322B1 (en) 2007-03-30 2013-02-27 Givaudan SA Methods to identify tas2r modulators
US7932058B2 (en) * 2007-06-08 2011-04-26 Senomyx, Inc. Rationale, methods, and assays for identifying human and non-human primate taste specific genes and use thereof in taste modulator and therapeutic screening assays
EP2195656A4 (en) 2007-08-21 2011-10-19 Senomyx Inc HUMAN T2R BITTERITY RECEPTORS AND USES THEREOF
US20100311610A1 (en) * 2008-02-01 2010-12-09 Chromocell Corporation CELL LINES AND METHODS FOR MAKING AND USING THEM (As Amended)
US20130115624A1 (en) 2010-05-07 2013-05-09 Givaudan S.A. Methods To Identify Modulators
US20130123135A1 (en) 2010-05-07 2013-05-16 Jay Patrick Slack Methods To Identify Modulators of TAS2R48 Receptors
EP2582367A4 (en) 2010-06-17 2013-11-27 California Inst Of Techn METHOD AND SYSTEM FOR MODULATING HORMONES AND RELATED METHODS, ACTIVE SUBSTANCES AND COMPOSITIONS
US9128079B2 (en) 2011-08-08 2015-09-08 The Coca-Cola Company Methods of using lung or bronchial epithelial cells to identify bitter taste modulators
WO2013159227A1 (en) * 2012-04-25 2013-10-31 University Of Manitoba Bitter taste receptor constitutively active mutants
US9804157B1 (en) 2013-03-15 2017-10-31 Senomyx, Inc. Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives
EP2983507B1 (en) 2013-03-15 2020-04-22 Applied Food Biotechnology, Inc. Feline bitter taste receptors and methods
US9169311B2 (en) 2013-03-15 2015-10-27 Applied Food Biotechnology, Inc. Feline bitter taste receptors and methods
JP6367315B2 (ja) 2013-05-10 2018-08-01 ペプシコ, インコーポレイテッドPepsiCo Inc. 潜在的味覚調節物質を特定するためのモデルとしての細胞
WO2014183041A1 (en) * 2013-05-10 2014-11-13 Pepsico, Inc. Taste receptor internalization assay
US9936723B2 (en) 2014-02-12 2018-04-10 Senomyx, Inc. Process for the synthesis of substituted 1-benzyl-3-(1-(isoxazol-4-ylmethyl)-1h-pyrazol-4-yl)imidazolidine-2,4-diones

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WO1993016178A2 (en) 1992-02-12 1993-08-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Sequences characteristic of human gene transcription product
NZ334517A (en) 1996-09-04 2000-03-27 Mogen Internat Nv Antifungal plant proteins and method of inhibiting fungal growth with carbohydrate oxidising activity and DNA coding sequences thereof
EP1056764A4 (en) 1998-02-18 2001-08-29 Genetics Inst SECRETED PROTEINS AND POLYNUCLEOTIDES ENCODING SAID PROTEINS
PT1139793E (pt) 1998-12-23 2010-01-19 Sinai School Medicine Inibidores da resposta ao sabor amargo
US6558910B2 (en) 1999-09-10 2003-05-06 The Regents Of The University Of California SF, a novel family of taste receptors
EP1214343A2 (en) 1999-09-10 2002-06-19 The Regents Of The University Of California T2r taste receptor family

Also Published As

Publication number Publication date
WO2001018050A9 (en) 2002-02-07
US7595166B2 (en) 2009-09-29
US7465550B2 (en) 2008-12-16
US20150260733A1 (en) 2015-09-17
US20180128842A1 (en) 2018-05-10
US8624012B2 (en) 2014-01-07
NO20021164L (no) 2002-05-10
CN1387536A (zh) 2002-12-25
AU7366400A (en) 2001-04-10
EP1214343A2 (en) 2002-06-19
US8329885B2 (en) 2012-12-11
US20120009598A1 (en) 2012-01-12
NO20021164D0 (no) 2002-03-08
JP2003510037A (ja) 2003-03-18
US20100062456A1 (en) 2010-03-11
JP4339542B2 (ja) 2009-10-07
IL148593A0 (en) 2002-09-12
WO2001018050A2 (en) 2001-03-15
US20050048586A1 (en) 2005-03-03
CA2384777A1 (en) 2001-03-15
US9063124B2 (en) 2015-06-23
WO2001018050A3 (en) 2002-01-10
US20130189712A1 (en) 2013-07-25
AU2004203826A1 (en) 2004-09-16
US20140120553A1 (en) 2014-05-01
US7868150B2 (en) 2011-01-11
US9817000B2 (en) 2017-11-14
US20080108088A1 (en) 2008-05-08
MXPA02002538A (es) 2005-07-01
AU773600B2 (en) 2004-05-27

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