CN1281123C - Poria wood grafting cultivation method - Google Patents

Abstract

The present invention relates to a method for manually breeding Tuckahoe, which comprises the following steps: selecting pine wood sections, selecting and digging fields, putting the pine wood sections into a cellar, culturing wood primers, manufacturing wood primers, inoculating wood primers, transmitting wood primers and separating mother seeds. Tuckahoe seeds cultured and propagated by the method have strong vitality, and is capable of effectively rejuvenating breeding tuckahoe. Through tests of several years, the tuckahoe generating rate of wood primers is high, sclerotium is large, the quality of the sclerotium is excellent, the yield can be commonly increased by 30%, and the method has the advantages of convenient operation as well as easy application and use.

Description

Poria wood grafting cultivation method

Technical field

The present invention relates to a kind of artificial poria cultivating method.

Background technology

Poria cocos is a kind of medicinal fungi, and the effect of have excreting dampness, diuresis, invigorating the spleen, calming the nerves is large medicinal material commonly used and exporting, the huge market demand.At present, China's tuckahoe production uses Poria cocos " meat draws " or pure mycelia kind to carry out vegetative propagation, owing to going down to posterity in producing and transplanting often, easily produce spawn degeneration, not only had a strong impact on Poria cocos output and quality, and often " pest cellar for storing things " occurred, caused damage, waste.

Summary of the invention

At above-mentioned deficiency, the object of the present invention is to provide the poria wood grafting cultivation method of a kind of rejuvenation kind Siberian cocklebur, raising Poria cocos output and quality.

To achieve these goals, technical scheme of the present invention is: poria wood grafting cultivation method is characterized in that realizing as follows: 1). choose pine tree Duan Mu; 2). selected scenes Wa Chang; 3). section wood is the cellar for storing things down; 4). wood draws cultivation; 5). wood draws making; 6). wood draws inoculation; 7). wood draws to pass and draws; 8). separate female the kind.

Concrete wooden grafting cultivation method is as follows:

1). choose pine tree Duan Mu: select diameter 4-6 centimetre, be sawn into the Duan Mu of 35-40 centimeter length after doing;

2). selected scenes Wa Chang: the southeast sandy soil in selected scenes is done the Siberian cocklebur field, and the Siberian cocklebur field depth digs 30-40 centimetre, and drainage trenching, turns over the grain on the threshing ground once before the following cellar for storing things;

3). section wood is the cellar for storing things down: when temperature is raised to more than 20 ℃, in the fine cellar for storing things down of choosing in the last ten-days period in April; The dryer section wood that saw is good is by every cellar for storing things 5.5-6.5 kilogram, divide two layers Duan Mu is layered in the cellar for storing things, store dark 22-28 centimetre, the kind Siberian cocklebur of choosing is broken into two with one's hands, every cellar for storing things restrains fresh poria cocos sclerotium with 400-500, Siberian cocklebur meat is close on the cross section of cellar for storing things upper end section wood, makes the Siberian cocklebur skin outside, the inoculation back covers 2-3 centimetre with sand;

4). wood draws cultivation: after the inoculation of section wood, the Siberian cocklebur field is put in order railway carriage or compartment Xiu Gou, prevented ponding, cultivated 50-60 days;

5). wood draws making: after treating that mycelia on the Duan Mu is covered with complete feed, the wood of maturation drawn dig out, be cut into the wood section of drawing of 17-20 centimeter length, for wood is introduced a fine variety;

6). wood draws inoculation: digs the cellar for storing things, stores dark 25-30 centimetre, and drainage trenching; Carry out wood mid-June and draw cellar for storing things down, with nonvaccinated pine tree section wood dress cellar for storing things, every cellar for storing things materials 7.5-8.0 kilogram, introduce a fine variety 2 with wood, wood is introduced a fine variety be clipped in the middle of the new Duan Mu, section links to each other, rubber band joins, every root segment wood is all introduced a fine variety adjointly with wood in the cellar for storing things, and earthing manages the Poria cocos of tying to 1 year then, is generation Siberian cocklebur;

7). wood draws to pass and draws: do a kind Siberian cocklebur with generation Siberian cocklebur and carry out meat and draw cultivation, the Poria cocos of knot be two generation Siberian cocklebur;

8). separate female the kind: with two generation Siberian cocklebur make the separate tissue material to carry out separation and Culture be the female kind of Poria cocos.

The present invention with pine tree Duan Mu in the Poria cocos field to store under the aquatic foods kind Siberian cocklebur, cultivate to " section die model " Poria cocos that covers with the Poria cocos mycelia and introduce a fine variety.Introduce a fine variety the Poria cocos of being tied with wood and do kind of a Siberian cocklebur, separation and Culture is the pure mycelia kind of Poria cocos, ectogenetic kind of Siberian cocklebur vitality of the present invention is vigorous, the Siberian cocklebur of rejuvenation kind effectively, through testing for many years: " wood connects and draws " tuckahoe production rate height, sclerotium is individual big, of fine quality, generally can increase production more than 30%, and easy to operate, be easy to apply.

Embodiment

For the Siberian cocklebur of rejuvenation kind effectively, improve Poria cocos output, quality, the present invention adopts host's rejuvenation method.

Concrete wooden grafting cultivation method is as follows:

1, choose Duan Mu: select diameter 4-6 centimetre, the less pine tree of joint crust is expected wood, is sawn into the Duan Mu of 40 centimeter length after doing, and saw kerf is avoided the joint crust, and section wooden length unanimity is moved on the Siberian cocklebur field of preparing inoculation standby then.

2, selected scenes Wa Chang: select soil layer deep, a southeast sandy soil is done the Siberian cocklebur field, removes native face foreign material when early spring, digs the Siberian cocklebur field, and 40 centimetres of Siberian cocklebur field depths along the ditch that begins to rehearse, slope, are turned over the grain on the threshing ground once before the following cellar for storing things then, put the Siberian cocklebur field in order.

3, Duan Mu stores down: when temperature is raised to more than 20 ℃, in the fine cellar for storing things down of choosing in the last ten-days period in April (21-30 day in April).The dryer section wood that saw is good is by 6 kilograms at every cellar for storing things (4-5 root material), divide two layers Duan Mu is layered in the cellar for storing things, store dark 25 centimetres, the kind Siberian cocklebur of choosing is broken into two with one's hands, every is close to Siberian cocklebur meat on the cross section of cellar for storing things upper end section wood with the fresh poria cocos sclerotium kind of 500 grams one cellar for storing things, makes the Siberian cocklebur skin outside, the inoculation back covers 2-3 centimetre with sand, does not make Duan Mu expose native face.

4, wood draws cultivation: after the section wood inoculation, with the whole railway carriage or compartment Xiu Gou in Siberian cocklebur field, prevent ponding, sprouting mycelia in 8-12 days in the Siberian cocklebur kind, just long mycelia was covered with full cellar for storing things in one month to Duan Mu, cultivated 50-60 days from back, cellar for storing things down, and wood is just introduced a fine variety can cultivate maturation.If meet long-term overcast and rainyly, available plastic film covers, but should open when day fine, otherwise influences mycelial growth.

5, wood draws making: after the mycelia of the section for the treatment of wood surface was covered with complete feed, " wood draws " section became faint yellow, and is wooden rotten soft, can Poria cocos fragrance be arranged to fractureing, i.e. expression " wood draws " maturation.The wood of maturation drawn dig out, be cut into the wood section of drawing of 20 centimeter length, Cheng Mu introduces a fine variety, and every cellar for storing things can be made wood and be introduced a fine variety the 8-10 piece.

6, wood draws inoculation: wood is introduced a fine variety down the cellar for storing things and is carried out in mid-June, with nonvaccinated pine tree section wood dress cellar for storing things, 8 kilograms of every cellar for storing things materials, introduce a fine variety 2 with wood, wood introduced a fine variety be clipped in the middle of the new Duan Mu, section is linked to each other, rubber band joins, every root segment wood is all introduced a fine variety adjointly with wood in the cellar for storing things, and earthing manages the Poria cocos of tying to 1 year and is " generation Siberian cocklebur " then.

7, wood draws to pass and draws: make the Siberian cocklebur kind with generation Siberian cocklebur and carry out " meat draws " and cultivate.The Poria cocos of knot be two generation Siberian cocklebur, " two generation Siberian cocklebur " have good high yield high-quality performance.

8, separate female the kind: making the separate tissue material with " two generation Siberian cocklebur ", to carry out separation and Culture be the female kind of Poria cocos, and this pure mycelia kind is called " wood connects and draws bacterial classification ", expands as original seed and cultivated species with this, and good effect of rejuvenation is arranged.

Claims (1)

1. poria wood grafting cultivation method is characterized in that realizing as follows:

1). choose pine tree Duan Mu: select diameter 4-6 centimetre, be sawn into the Duan Mu of 35-40 centimeter length after doing;

2). selected scenes Wa Chang: the southeast sandy soil in selected scenes is done the Siberian cocklebur field, and the Siberian cocklebur field depth digs 30-40 centimetre, and drainage trenching, turns over the grain on the threshing ground once before the following cellar for storing things:

3). section wood is the cellar for storing things down: when temperature is raised to more than 20 ℃, in the fine cellar for storing things down of choosing in the last ten-days period in April; The dryer section wood that saw is good is by every cellar for storing things 5.5-6.5 kilogram, divide two layers Duan Mu is layered in the cellar for storing things, store dark 22-28 centimetre, the kind Siberian cocklebur of choosing is broken into two with one's hands, every cellar for storing things restrains fresh poria cocos sclerotium with 400-500, Siberian cocklebur meat is close on the cross section of cellar for storing things upper end section wood, makes the Siberian cocklebur skin outside, the inoculation back covers 2-3 centimetre with sand;

4). wood draws cultivation: after the inoculation of section wood, the Siberian cocklebur field is put in order railway carriage or compartment Xiu Gou, prevented ponding, cultivated 50-60 days;

5). wood draws making: after treating that mycelia on the Duan Mu is covered with complete feed, the wood of maturation drawn dig out, be cut into the wood section of drawing of 17-20 centimeter length, for wood is introduced a fine variety;

6). wood draws inoculation: digs the cellar for storing things, stores dark 25-30 centimetre, and drainage trenching; Carry out wood mid-June and draw cellar for storing things down, with nonvaccinated pine tree section wood dress cellar for storing things, every cellar for storing things materials 7.5-8.0 kilogram, introduce a fine variety 2 with wood, wood is introduced a fine variety be clipped in the middle of the new Duan Mu, section links to each other, rubber band joins, every root segment wood is all introduced a fine variety adjointly with wood in the cellar for storing things, and earthing manages the Poria cocos of tying to 1 year then, is generation Siberian cocklebur;

7). wood draws to pass and draws: do a kind Siberian cocklebur with generation Siberian cocklebur and carry out meat and draw cultivation, the Poria cocos of knot be two generation Siberian cocklebur;

8). separate female the kind: with two generation Siberian cocklebur make the separate tissue material to carry out separation and Culture be the female kind of Poria cocos.