CN105638233A

CN105638233A - Cultivation method for Poria cocos strains

Info

Publication number: CN105638233A
Application number: CN201410668203.8A
Authority: CN
Inventors: 杨晓波
Original assignee: Wulong County Xiaobo Chinese Herbal Medicine Planting Professional Cooperatives
Current assignee: Wulong County Xiaobo Chinese Herbal Medicine Planting Professional Cooperatives
Priority date: 2014-11-21
Filing date: 2014-11-21
Publication date: 2016-06-08

Abstract

The invention relates to a cultivation method for Poria cocos strains, and the method can solve the problem that the conventional cultivation method is great in wood consumption, is large in land occupation, and is poor in finished product quality. The method is characterized in that: 70-95% of a primary material of a Poria cocos culture medium and 10-25% of a secondary material of the Poria cocos culture medium are evenly mixed and are putted into bags, and then sterilization is performed on the material bags, and the material bags serve as the culture medium; the Poria cocos strains are planted on the culture medium in bags, and then the bags are transferred to a culture room to culture at the temperature of 22-32 DEG C for about 8-13 days; after hyphae overgrow all the bags, the lower ends of the bags are cut, and 10 grams of fresh Poria cocos sclerotium is planted in cutting openings of the lower ends of the bags; the bags are vertically arranged in a sand pool with the thickness of 10 cm in the room and are continuously cultured at the temperature of 15-30 DEG C; it is checked that the sclerotium is survival or not after a week; the bags with the survival sclerotium are intensely and vertically arranged in the sand pool in the room and are continuously cultured at the temperature of 15-30 DEG C for three months; and the bags are took out until the sclerotium stops growing, and Poria cocos products are obtained. The method has the beneficial effects that the method is simple in process, is easy to manage, is not limited by weather conditions, is short in production period, is high in yield of Poria cocos, can increase the resource utilization rate by over 30%, and is easy to promote and apply.

Description

The method of cultivation of a kind of Poria cocos bacterial classification

Technical field

The present invention relates to the method for cultivation of a kind of Poria cocos bacterial classification.

Background technology

Poria cocos is a kind of polyporaceae medicinal fungi, effects such as there is excreting dampness, diuresis, be good for spleen, calm the nerves, can control dysuria, that oedema is swollen is full, phlegm and retained fluid coughs inverse, inverse, the vomiting during early pregnancy of vomitting, have loose bowels, pass out semen, drench turbid, palpitation with fear, the disease such as forgetful, for large modern Chinese herbal medicine raw medicinal material, domestic and international demand constantly increases. at present, the traditional cultivating method of China's Poria cocos is mainly outdoor to be buried saprophytic with dry and soft wood hole, Poria cocos " meat cited " or pure mycelia kind is used to carry out vegetative propagation, need to through digging pit, trench digging, bury Siberian cocklebur, inoculation, earthing, the operations such as spraying medicine, the method operation is loaded down with trivial details, easily produce spawn degeneration, take arable land and consume timber many, by weather influence, cost height, output is low, and various miscellaneous bacteria and insect pest are many in soil, need medical treatment, contaminate environment, the serious seed output and quality affecting Poria cocos, constrain the development of tuckahoe production long-term sustainable, the growing Chinese materia medica market requirement can not be met.

Summary of the invention

In view of the above-mentioned problems in the prior art, the main purpose of the present invention is to address the deficiencies of the prior art, the present invention provides the method for cultivation of a kind of Poria cocos bacterial classification, for saving timber and land resources, simplification production process, shorten the production cycle, reduce sick worm harm, indoor cultivation, be convenient to implement and management, thus improve Poria cocos seed output and quality, produce nuisanceless Poria cocos.

The present invention provides the method for cultivation of a kind of Poria cocos bacterial classification, and its technical scheme is as follows:

1, getting the raw materials ready: Poria cocos substratum mixes in major ingredient 70��95%, auxiliary material 10��25% ratio, and often packed 0.6��1.2kg, loads onto the collar, beyond the Great Wall tampon, normal-pressure sterilization 12 hours or 120 DEG C of autoclavings 1��2.5 hour, take out to be put in and totally locate spreading for cooling; The standby Poria cocos bacterial classification cultivated; Described base-material is by diameter 5��15 centimetres, long 30 centimetres of dry and soft juggle centrally cross splittings, then puts into 2.5% sucrose water and soaks 12 hours, and soak solution PH value 5.0��6.5, pulls out;Described auxiliary material is wheat bran, pine sawdust, magnesium sulfate and potassium primary phosphate;

2, inoculate: the Poria cocos bacterial classification cultivated is inoculated on the section wood culture medium cooled, every bag of inoculum size 5��16g;

3, cultivate: the bacterium bag of inoculation is proceeded within the scope of 22��32 DEG C, culturing room inherence and cultivate about 8��13 days, treat that mycelium covers with full bag and takes out;

4, draw on: lower for bacterium bag end plastics are scratched, connect the fresh poria cocos sclerotium of a fritter 5��16g in cut place, then plastics bag is vertically put into and be lined with the thick Sha Chizhong of 5��16cm, sclerotium and sand and bag wood are nuzzled, continue to cultivate at 15��30 DEG C, after one week, check whether sclerotium survives;

5, manage: the bacterium bag survived by sclerotium is concentrated and stood up in indoor sand pond, continue to cultivate at 15��30 DEG C, spray cooling in atmosphere during sky heat, when material wood is crossed dry in bag, open tampon Additional nutrient solution;

6, gathering: cultivate about three months, treat sclerotium not regrowth, section wood hand is pinched namely broken, when color becomes brown, namely adopts lower sclerotium, takes advantage of Poria cocos crust under fresh stripping, be cut into bulk, sheet or fourth shape, in 65��70 DEG C of oven dry, obtain Poria cocos finished product.

The present invention has the following advantages and useful effect: not only operation is simple, it is convenient to implement and management, not by weather conditionality, with short production cycle, tuckahoe production rate height, nuisanceless sclerotium product are of fine quality, and timber and land resources can be saved, reduce sick worm harm, there is the advantages such as cost is low, economic benefit height, resource utilization more than 30% can be improved, it is easy to apply.

Embodiment

The method of cultivation of a kind of Poria cocos bacterial classification of the embodiment of the present invention:

Embodiment 1: first by diameter 12 centimetres, long 25 centimetres of dry and soft juggle centrally cross splittings, totally 100 sections, then juggle is put into 2.5% sucrose water immersion 11 hours, soak solution PH value 5.5��6.5, pulls out; Then wheat bran 3kg, pine sawdust 3.5kg, magnesium sulfate, each 4g of potassium primary phosphate is added, mix thoroughly, it is respectively charged into acrylic plastering bag, often packed one piece of Duan Mu, the middle infiltration auxiliary material such as wheat bran, wood chip, every packed 0.8kg, load onto the collar, beyond the Great Wall tampon, it is placed in 120 DEG C of autoclavings 1 hour, taking-up is put in clean place, and spreading for cooling is stand-by. The Poria cocos bacterial classification cultivated is inoculated in sterilisable chamber or inoculation railway carriage or compartment on the section wood culture medium cooled, every bag of inoculum size 10g. The bacterium bag of inoculation is proceeded within the scope of 22��32 DEG C, culturing room inherence and cultivate about 8��13 days, treat that mycelium covers with full bag, lower for bacterium bag end plastics are scratched, the fresh poria cocos sclerotium of a fritter 10g is connected in cut place, then plastics bag is vertically put into and it is lined with the thick Sha Chizhong of 10cm, sclerotium and sandy soil and bag wood are nuzzled, continues to cultivate at 15��30 DEG C, after one week, check whether sclerotium survives. The bacterium bag survived by sclerotium again is concentrated and is stood up in indoor sand pond, continues to cultivate at 15��30 DEG C, and spray cooling in atmosphere during sky heat opens tampon Additional nutrient solution when material wood is crossed dry in bag. Cultivating about three months, treat sclerotium not regrowth, section wood hand is pinched namely broken, when color becomes brown, namely adopts lower sclerotium, takes advantage of Poria cocos crust under fresh stripping, be cut into bulk, sheet, in 65��70 DEG C of oven dry, obtain Poria cocos finished product.

Embodiment 2: first by diameter 6 centimetres, the dry and soft juggle centrally cross splitting of long 25 centimetres, then juggle is put into 2.5% sucrose water immersion 12 hours, soak solution PH value 6.0��6.5, pulls out, adds the auxiliary material such as wheat bran, wood chip, every packed 1kg.Bag Material ratio, by pine 88%, pine sawdust 5%, wheat bran 7%, separately joins magnesium sulfate 0.6g for every bag, and potassium primary phosphate 1g, mixes, and loads onto the collar, beyond the Great Wall tampon, normal-pressure sterilization 12 hours, takes out to put into and totally locates spreading for cooling. Being inoculated in sterilisable chamber or inoculation railway carriage or compartment on the section wood culture medium cooled by the Poria cocos bacterial classification cultivated, every bag of inoculum size is about 10g again. The bacterium bag of inoculation is proceeded within the scope of 22��32 DEG C, culturing room inherence and cultivate about 8��13 days, treat that mycelium covers with full bag, lower for bacterium bag end plastics are scratched, a fresh poria cocos sclerotium of fritter is connected in cut place, weight is about 10g, is then vertically put into by plastics bag and is lined with the thick Sha Chizhong of 12cm, and sclerotium and sand and bag wood are nuzzled, continue to cultivate at 15��30 DEG C, after one week, check whether sclerotium survives. By on the bacterium bag that draws concentrate and stand up in indoor sand pond, continue to cultivate at 15��30 DEG C, spray cooling in atmosphere during sky heat, can open tampon Additional nutrient solution when material wood is crossed dry in bag. Cultivating about three months, treat sclerotium not regrowth, section wood hand is pinched namely broken, when color becomes brown, namely adopts lower sclerotium. Take advantage of Poria cocos crust under fresh stripping, it is cut into bulk, sheet or fourth shape, in 65��70 DEG C of oven dry, obtains Poria cocos finished product.

Last it is noted that above-described each embodiment is only for illustration of the technical scheme of the present invention, it is not intended to limit; Although with reference to previous embodiment to invention has been detailed description, it will be understood by those within the art that: the technical scheme described in previous embodiment still can be modified by it, or wherein part or all of technology feature is carried out equivalent replacement; And these amendments or replacement, do not make the scope of the essence disengaging various embodiments of the present invention technical scheme of appropriate technical solution.

Claims

1. the method for cultivation of a Poria cocos bacterial classification, it is characterised in that: comprise the steps:

A, getting the raw materials ready: Poria cocos substratum mixes in major ingredient 70��95%, auxiliary material 10��25% ratio, and often packed 0.6��1.2kg, loads onto the collar, beyond the Great Wall tampon, normal-pressure sterilization 12 hours or 120 DEG C of autoclavings 1��2.5 hour, take out to be put in and totally locate spreading for cooling; The standby Poria cocos bacterial classification cultivated; Described base-material is by diameter 5��15 centimetres, long 30 centimetres of dry and soft juggle centrally cross splittings, then puts into 2.5% sucrose water and soaks 12 hours, and soak solution PH value 5.0��6.5, pulls out; Described auxiliary material is wheat bran, wood chip, magnesium sulfate and potassium primary phosphate;

B, inoculation: the Poria cocos bacterial classification cultivated is inoculated on the section wood culture medium cooled, every bag of inoculum size 5��16g;

C, cultivation: proceeded to by the bacterium bag of inoculation within the scope of 22��32 DEG C, culturing room inherence and cultivate about 8��13 days, treat that mycelium covers with full bag and takes out;

D, on draw: lower for bacterium bag end plastics are scratched, connect the fresh poria cocos sclerotium of a fritter 5��16g in cut place, then plastics bag is vertically put into and be lined with the thick Sha Chizhong of 5��16cm, sclerotium and sand and bag wood are nuzzled, continue to cultivate at 15��30 DEG C, after one week, check whether sclerotium survives;

E, management: the bacterium bag survived by sclerotium is concentrated and stood up in indoor sand pond, continue to cultivate at 15��30 DEG C, and spray cooling in atmosphere during sky heat opens tampon Additional nutrient solution when material wood is crossed dry in bag;

F, gathering: cultivate about three months, treat sclerotium not regrowth, section wood hand is pinched namely broken, when color becomes brown, namely adopts lower sclerotium, takes advantage of Poria cocos crust under fresh stripping, is cut into bulk, sheet or fourth shape, in 65��70 DEG C of oven dry, obtains Poria cocos finished product.