Background technology
Grifola frondosus [Grifola frondosa (Dicks.ex Fr) S.F.Gray], have another name called dance fine and soft, belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, tree Pseudomonas.Being the famous and precious economic kind that WHO and FAO tissue are recommended to developing country, is the rare edible mushroom of top grade of a kind of meals, medicine dual-purpose.
China carries out artificial domesticating cultivation to grifola frondosus since early 1980s, there are Hebei and Zhejiang in the larger province of cultivation at present, the artificial cultivation that other province is as micro-as Liaoning, Shandong, Shanxi, Shaanxi, peace, also there is grifola frondosus in Jiangsu, Hubei, Fujian etc., but scale is all little.Batch production production technology research China of grifola frondosus starts late, at present still in the starting stage.
In grifola frondosus is produced, during especially batch production is produced, fast, neatly, efficiently urge flower bud, be to produce one of successful key technology.The present invention is going out the flower bud rate, is going out flower bud speed and go out on the flower bud regularity and urge the flower bud technology to compare with tradition, and advantage is remarkable, and can realize urging flower bud than under low relative humidity, is beneficial to ventilation, is applicable to batch production production.
Scarfing urges the flower bud technical method to be: (1) scarfing, mycelia was covered with full bag after 10~20 days, selected the dense part of mycelial growth, with sharp small blade, on the cylinder bag, cut out
shape fruiting hole, every edge lengths is 1.5~2.0cm, and scrapes off mycoderma and a little composts or fertilisers of cultivating at scarfing place, dark approximately 2~3mm, 1~3 of each bacterium rod scarfing, after scarfing by bacterium rod laid parallel on the ground or layer frame of mushroom room; (2) urge flower bud, after bacterium rod scarfing, should increase in time the mushroom room air humidity, keep relative air humidity 90~95%, 15~22 ℃ of temperature, temperature constant state, and give the illumination of 50~200LUX; 6~8d after scarfing, can form former base white thrust at the scarfing place; Now should increase in time illumination, give the intensity of illumination of 200~500LUX, temperature and humidity remain unchanged, and impel former base progressively to transfer grey black to, and differentiate gradually young flower bud.
Its shortcoming is: (1) urges not high, the young flower bud of flower bud rate irregular, and mycelia is more responsive to environment, and, by the control of overall situation, is difficult to make each bacterium rod fruiting subenvironment height consistent; (2) humidity of having relatively high expectations when grifola frondosus is urged flower bud and throughput can affect throughput when whole mushroom room humidification, and environment control difficulty is larger.
Earthing urges the flower bud technical method to be: (1) cover soil material is prepared, and at earthing, within first 10 days, starts to prepare cover soil material, covers Biao Tuhuo field, the mountain subsoil that can select sand with soil, and soils particles size percent content is below 1cm; Earthing carries out soil disinfection and regulates water content in first 7 days, method is to admix 1% lime in soil, and sprays the insecticide of edible mushroom safety in production license, regulates water content to 45% left and right, then play heap, epiphragma, more stand-by after 7 days with the aerial fog disinfectant vexed heap of sterilizing; (2) earthing method, first select the place at leeward nearly water source, every 60cm, digs the furrow ditch that 50cm is wide, 25cm is dark, and the furrow ditch is generally east-west, long 3 meters; Then fill with flood 1 time, after water oozes, spread skim lime, then the outer layer plastic bag of the bacterium bag of sending out bacterium good is all sloughed, vertically be emitted in the furrow ditch; During discharge, bacterium holds in both hands and all should retain the gap of 1~2cm all around, and on the bacterium rod, end face is than the low 2cm of furrow stalk; Insert above-mentioned cover soil material in the gap of bacterium rod surrounding, again at bacterium rod surface coverage one deck, thickness is 1.5~2.0cm subsequently; Finally waddy or bamboo pole are ridden on furrow, be covered with Polypropylence Sheet, then cover straw screen or mat, purpose is temperature adjustment and shelters from heat or light, and ventilation hole should be left in two ends; (3) urge flower bud management, should increase in time humidity in the fruiting canopy after earthing, keep relative moisture in 90% left and right, 15~22 ℃ of temperature, and award the scattered light of 200~500LUX.
Its shortcoming is: (1) mushroom product are easily by soil pollution, easy cleaning not, and commodity value is not high; (2) take more soil, be not suitable with the batch production Production requirement; (3) preparing from cover soil material, to the earthing operation, expend more manually, and is heavier manual labor; (4) due to after earthing, each bacterium rod subenvironment is difficult to accurate control, urges flower bud to be difficult to concentrate, and causes fruiting irregular, and the fruiting phase is longer.
Summary of the invention
Technical problem to be solved by this invention is: determine best fruiting point and the suitableeest fruiting area by scarfing, by covering, watt construct the more consistent flower bud subenvironment of urging, can fast, unanimously, efficiently urge out high-quality mushroom flower bud.
Solving the technical scheme that its technical problem adopts is:
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, comprises that bacterium packs work, inoculation cultivation, tile making, the differentiation of former base, educates the mushroom method, is characterized in that:
The tile preparation method is: tile is shown laid flat in half elliptic, oval major diameter is 3/4~1 times of bacterium bag diameter, minor axis is 3/8~1/2 of bacterium bag diameter, minor axis is 1/3~2/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 1/2~4/5 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 5~10 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2~2.5cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 2~3mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 17~19 ℃, humidity is controlled at 75~80%, CO
2concentration is controlled at below 1500mg/kg, and gives the illumination of 200~800LUX,
Former base differentiation method is: cover and watt urge flower bud 8~10 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 18~20 ℃, and humidity is controlled at 85~90%, CO
2concentration is controlled at below 1000mg/kg, and gives the illumination of 400~1000LUX.
Described bacterium packs as method: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing.
Described inoculation cultivation method is: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 25~30 days.
The described mushroom method of educating is: cover and watt urge flower bud 15~16 days, temperature is controlled at 16~24 ℃, and humidity is controlled at 85% left and right, CO
2concentration is controlled at below 1500mg/kg, and gives the illumination of 400~1000LUX, until gather.
The invention has the beneficial effects as follows: the grifola frondosus mycelia has obvious after-ripening characteristic, originally mycelia is more sparse, along with After-mature cultivation, mycelia is long dense gradually, simultaneously, very strict to the fruiting area requirements during grifola frondosus fruiting, excessively too smallly all can affect mushroom shape and output, the fruiting hole that can select the dense place of mycelia to open suitable size during opening, lay the first stone for urging out high-quality mushroom flower bud; When grifola frondosus children flower bud forms, stricter to environmental requirement, build the more consistent flower bud subenvironment of urging by covering watt, mushroom room humidity requirement lower (75~80%), and the requirement of routine techniques relative moisture is 90~95%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, reduced the energy resource consumption controled environment, can fast, unanimously, efficiently urge out high-quality mushroom flower bud, also can increase unit space bacterium containing amount, be beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease, indirectly increases production 5~10%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.
Embodiment
Embodiment 1
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, and its method of operating is as follows:
(1) bacterium packs work: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing;
(2) inoculation cultivation: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 25 days;
(3) tile is made: tile is shown laid flat in half elliptic, oval major diameter is 3/4 times of bacterium bag diameter, minor axis is 3/8 of bacterium bag diameter, minor axis is 1/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 1/2 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 5 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 2mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 19 ℃, humidity is controlled at 80%, CO
2concentration is controlled at 1000mg/kg, and gives the illumination of 800LUX,
(4) former base differentiation: cover and watt urge flower bud 8 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 18 ℃, and humidity is controlled at 90%, CO
2concentration is controlled at 1000mg/kg, and gives the illumination of 1000LUX;
(5) educate mushroom: cover and watt urge flower bud 15 days, temperature is controlled at 24 ℃, and humidity is controlled at 85% left and right, CO
2concentration is controlled at 1500mg/kg, and gives the illumination of 1000LUX, until gather.
Urge the flower bud subenvironment by covering a watt structure, the mushroom room humidity requirement is low by 80%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, has reduced the energy resource consumption controled environment, and also can increase unit space bacterium containing amount, is beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease simultaneously, indirectly increases production 6%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.
Embodiment 2
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, and its method of operating is as follows:
(1) bacterium packs work: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing;
(2) inoculation cultivation: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 30 days;
(3) tile is made: tile is shown laid flat in half elliptic, oval major diameter is 1 times of bacterium bag diameter, minor axis is 1/2 of bacterium bag diameter, minor axis is 2/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 4/5 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 10 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 3mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 19 ℃, humidity is controlled at 75%, CO
2concentration is controlled at 1500mg/kg, and gives the illumination of 200LUX,
(4) former base differentiation: cover and watt urge flower bud 10 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 18 ℃, and humidity is controlled at 90%, CO
2concentration is controlled at 800mg/kg, and gives the illumination of 1000LUX;
(5) educate mushroom: cover and watt urge flower bud 16 days, temperature is controlled at 24 ℃, and humidity is controlled at 85%, CO
2concentration is controlled at 1500mg/kg, and gives the illumination of 400LUX, until gather.
Urge the flower bud subenvironment by covering a watt structure, the mushroom room humidity requirement is low by 75%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, has reduced the energy resource consumption controled environment, and also can increase unit space bacterium containing amount, is beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease simultaneously, indirectly increases production 10%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.
Embodiment 3
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, and its method of operating is as follows:
(1) bacterium packs work: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing;
(2) inoculation cultivation: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 27 days;
(3) tile is made: tile is shown laid flat in half elliptic, oval major diameter is 4/5 times of bacterium bag diameter, minor axis is 5/8 of bacterium bag diameter, minor axis is 1.5/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 4/5 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 8 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2.3cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 2.5mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 18 ℃, humidity is controlled at 78%, CO
2concentration is controlled at 600mg/kg, and gives the illumination of 500LUX,
(4) former base differentiation: cover and watt urge flower bud 9 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 19 ℃, and humidity is controlled at 87%, CO
2concentration is controlled at below 500mg/kg, and gives the illumination of 700LUX;
(5) educate mushroom: cover and watt urge flower bud 15.5 days, temperature is controlled at 20 ℃, and humidity is controlled at 85%, CO
2concentration is controlled at 1000mg/kg, and gives the illumination of 700LUX, until gather.
Urge the flower bud subenvironment by covering a watt structure, the mushroom room humidity requirement is low by 78%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, has reduced the energy resource consumption controled environment, and also can increase unit space bacterium containing amount, is beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease simultaneously, indirectly increases production 10%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.
Embodiment 4
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, and its method of operating is as follows:
(1) bacterium packs work: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing;
(2) inoculation cultivation: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 26 days;
(3) tile is made: tile is shown laid flat in half elliptic, oval major diameter is 3/4 times of bacterium bag diameter, minor axis is 5/8 of bacterium bag diameter, minor axis is 2/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 1/2 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 9 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2.2cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 2mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 17 ℃, humidity is controlled at 75%, CO
2concentration is controlled at below 800mg/kg, and gives the illumination of 200LUX,
(4) former base differentiation: cover and watt urge flower bud 8 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 20 ℃, and humidity is controlled at 90%, CO
2concentration is controlled at 1000mg/kg, and gives the illumination of 1000LUX;
(5) educate mushroom: cover and watt urge flower bud 15 days, temperature is controlled at 16 ℃, and humidity is controlled at 85%, CO
2concentration is controlled at 1500mg/kg, and gives the illumination of 400LUX, until gather.
Urge the flower bud subenvironment by covering a watt structure, the mushroom room humidity requirement is low by 75%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, has reduced the energy resource consumption controled environment, and also can increase unit space bacterium containing amount, is beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease simultaneously, indirectly increases production 5%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.
Embodiment 5
A kind of grifola frondosus bag cultivation scarfing covers a watt formula urges the flower bud method, and its method of operating is as follows:
(1) bacterium packs work: by the formulated Grifola frondosa culture material, the dress bag, with collar sealing, sterilizing;
(2) inoculation cultivation: the bacterium bag that sterilizing is complete is cooled to room temperature, by sterile working access grifola frondosus three-class strain, puts into baterial cultivation chamber and cultivates and cover with the bacterium bag to mycelia in 28 days;
(3) tile is made: tile is shown laid flat in half elliptic, oval major diameter is 1 times of bacterium bag diameter, minor axis is 3/8 of bacterium bag diameter, minor axis is 1/3 of major diameter, by ellipse curved be tile shape, tile arc diameter is 4/5 of bacterium bag diameter, being about to tile is overlying on the bacterium bag, form a space because of diameter missionary society between tile and bacterium bag, mycelia is covered with by After-mature cultivation 10 days, the bacterium bag is moved into to mushroom room, open the circular hole of diameter 2cm as the fruiting hole at the dense place of bacterium bag sidewall mycelia, cut composts or fertilisers of cultivating in hole to pieces 3mm, the bacterium bag is lain against on ground or shelf, in the hole cover tiles, temperature is controlled at 17 ℃, humidity is controlled at 75%, CO
2concentration is controlled at 1000mg/kg, and gives the illumination of 200LUX,
(4) former base differentiation: cover and watt urge flower bud 10 days, the fruiting hole grows the former base of brain shape, removes to cover watt, and temperature is controlled at 20 ℃, and humidity is controlled at 85%, CO
2concentration is controlled at 500mg/kg, and gives the illumination of 400LUX;
(5) educate mushroom: cover and watt urge flower bud 16 days, temperature is controlled at 24 ℃, and humidity is controlled at 80%, CO
2concentration is controlled at below 500mg/kg, and gives the illumination of 1000LUX, until gather.
Urge the flower bud subenvironment by covering a watt structure, the mushroom room humidity requirement is low by 75%, has alleviated and has urged the contradiction that simultaneously increases humidity and throughput in the flower bud process, has reduced the energy resource consumption controled environment, and also can increase unit space bacterium containing amount, is beneficial to batch production production; Lower relative moisture can significantly reduce the generation of grifola frondosus bacterium and fungal disease simultaneously, indirectly increases production 7%; Can select the suitableeest flower bud point of urging during scarfing, effectively control the fruiting area, fruiting mushroom shape is attractive in appearance, in the same size, and product are of fine quality; Adopt the present invention to urge flower bud mushroom product clean and tidy than earthing, the commodity performance is good simultaneously.