CN113348968A - Preparation of stropharia rugoso-annulata comprehensive culture medium and stropharia rugoso-annulata cultivation method - Google Patents
Preparation of stropharia rugoso-annulata comprehensive culture medium and stropharia rugoso-annulata cultivation method Download PDFInfo
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- CN113348968A CN113348968A CN202110787635.0A CN202110787635A CN113348968A CN 113348968 A CN113348968 A CN 113348968A CN 202110787635 A CN202110787635 A CN 202110787635A CN 113348968 A CN113348968 A CN 113348968A
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- 241000958510 Stropharia rugosoannulata Species 0.000 title claims abstract description 56
- 239000001963 growth medium Substances 0.000 title claims abstract description 31
- 238000012364 cultivation method Methods 0.000 title claims description 12
- 238000002360 preparation method Methods 0.000 title description 5
- 239000000463 material Substances 0.000 claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 7
- 229930006000 Sucrose Natural products 0.000 claims abstract description 7
- 239000005720 sucrose Substances 0.000 claims abstract description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 4
- 230000035622 drinking Effects 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 239000002689 soil Substances 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 13
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 8
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 7
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 7
- 239000004571 lime Substances 0.000 claims description 7
- 238000009331 sowing Methods 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 229960004793 sucrose Drugs 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 3
- 235000019764 Soybean Meal Nutrition 0.000 claims description 3
- 238000009825 accumulation Methods 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 210000005069 ears Anatomy 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 238000010565 inoculated fermentation Methods 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 239000010410 layer Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- ATROHALUCMTWTB-OWBHPGMISA-N phoxim Chemical compound CCOP(=S)(OCC)O\N=C(\C#N)C1=CC=CC=C1 ATROHALUCMTWTB-OWBHPGMISA-N 0.000 claims description 3
- 229950001664 phoxim Drugs 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000007493 shaping process Methods 0.000 claims description 3
- 239000004455 soybean meal Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000002344 surface layer Substances 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 240000007049 Juglans regia Species 0.000 claims description 2
- 235000009496 Juglans regia Nutrition 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 235000020234 walnut Nutrition 0.000 claims description 2
- 241000610743 Psathyrotes ramosissima Species 0.000 claims 1
- 238000007796 conventional method Methods 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 4
- 241000610761 Psathyrotes Species 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a stropharia rugoso-annulata comprehensive culture medium which comprises a basic culture solution, wherein the basic culture solution is prepared by decocting fresh stropharia rugoso-annulata fruiting bodies which are added with a mass unit of 1.5% and meet the drinking standard of domestic water, and adding 1.0-2.0% of sucrose, 0.10-0.50% of protein powder and 0.20-0.30% of yeast powder into the basic culture solution, the invention breaks through the technical modes of single nutrition of the traditional culture base material and long strain period manufactured by the conventional method, overcomes the defects of short fruiting period and low yield, adopts liquid strains to manufacture culture strains, utilizes the comprehensive culture base material to cultivate the stropharia rugosoannulata, and practices prove that, in cold temperate areas, compared with the conventional method for cultivating stropharia rugoso-annulata, the stropharia rugoso-annulata cultivated by the technology is cultivated under the natural climate conditions, the artificial cultivation can be implemented 15 days ahead of time, the fruiting period is prolonged by about 20 days, and the biological conversion rate can be improved by 15-20%.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a preparation method of a stropharia rugoso-annulata comprehensive culture medium and a stropharia rugoso-annulata cultivation method.
Background
The existing technology for cultivating stropharia rugoso-annulata comprises the following steps: preparing a mother seed by a PDA culture medium → preparing a subculture mother seed by the PDA culture medium → transferring a solid culture medium to produce a cultivated seed → cultivating by a conventional culture medium; the defects are shown in the following aspects: 1. the whole process from the preparation of mother seeds by the PDA culture medium to the implementation of cultivation needs about 70 days, and 2, the yield is low due to single nutrition of the culture medium.
Disclosure of Invention
The invention aims to provide a stropharia rugoso-annulata comprehensive culture medium and a stropharia rugoso-annulata cultivation method to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a stropharia rugoso-annulata comprehensive culture medium comprises a basic culture solution, wherein the basic culture solution is prepared by decocting fresh stropharia rugoso-annulata sporocarp which is 1.5 mass unit of the domestic water drinking standard and is added with 1.0-2.0 mass unit of sucrose, 0.10-0.50% of protein powder, 0.20-0.30% of yeast powder, 0.6-0.7% of soybean meal, 0.05-0.10% of dipotassium hydrogen phosphate and 0.05-0.10% of magnesium sulfate, the mixture is uniformly mixed, the pH value is adjusted to 6.3-6.8, and the culture is carried out after sterilization and cooling.
A stropharia rugoso-annulata cultivation method comprises the following steps:
step 1, transferring the stropharia rugoso-annulata mother seeds to a test tube containing a PDA culture medium for activation, recovering the activity of hyphae of the stropharia rugoso-annulata mother seeds, and then cutting to obtain original stropharia rugoso-annulata PDA bacterial blocks;
step 2, inoculating the PDA bacterial blocks of the stropharia rugoso-annulata stock seeds to a sterilized PDB liquid culture medium, and culturing for 5-7 days in the dark by using a shaking table at the temperature of 22-26 ℃ to obtain primary stropharia rugoso-annulata seeds;
step 3, preparing liquid second-level seeds: inoculating the first-class strain of Stropharia rugosoannulata into a fermentation tank containing a comprehensive culture medium of the Stropharia rugosoannulata, and then culturing the inoculated fermentation tank at 22-26 ℃ for 4-5 days;
step 4, preparing solid culture strains;
step 5, preparing a comprehensive cultivation base material;
and 6, making a bed, paving materials, sowing and paving materials in the greenhouse.
As a further technical scheme of the invention: the step 4 specifically comprises the following steps: inoculating the obtained liquid secondary strain of the stropharia rugoso-annulata to a solid strain culture medium, and culturing for 40 days at the temperature of 22-26 ℃ in the dark, wherein the solid strain culture medium is prepared from 78% of mushroom bran, 20% of wheat bran, 1% of cane sugar and 1% of lime; preparing according to conventional strain production, inoculating liquid strain cultured for 4-5 days, and culturing for 40 days to obtain solid culture strain.
As a further technical scheme of the invention: the step 5 specifically comprises the following steps: separating and crushing 70% of bag materials of waste fungus bags with over-mushrooms and ears, cutting forest weeds into small sections with the length of 5-8cm and the length of 30%, mixing the two materials, adjusting the water content to 65-68% and the pH value to 5-7, and then performing 70 ℃ stacking fermentation treatment on the synthesized comprehensive cultivation base material.
As a further technical scheme of the invention: the bed making method comprises the following specific steps: the width of the bed is 1.3 meters, the soil is firstly dug to the ground and taken out with the depth of 3-4 centimeters, the soil is placed on an operation way at intervals of the ridge beds for covering soil, the bed surface is trimmed to be a turtle back type with a slightly higher middle part to prevent water accumulation at the bottom of the bed, lime powder and phoxim are sprayed on the bed surface, and when the water regulation of the culture material reaches 65 percent, the material temperature is reduced to be below 25 ℃, the material spreading is started.
As a further technical scheme of the invention: the concrete steps of the paving material are as follows: firstly, the material is paved about 8-10 cm in thickness and 1.2 m in width, then a material bed of 1.2 m is divided into two ridges, the distance between the two ridges is about 12 cm, the south and north ends of the two ridges are sealed by the material, the material feeding amount is increased, the mushroom yield is increased, and the irrigation is facilitated after the two sides of the ridge and the ditch are plugged.
As a further technical scheme of the invention: the seeding method comprises the following specific steps: dividing strains into walnut blocks, transversely playing three holes in each small single ridge, wherein the distance between the blocks is 8-10 cm, dibbling three rows in sequence along the ridges, the distance between the blocks is 8-10 cm, after the first-layer sowing is finished, paving culture materials with the thickness of 8-10 cm on each single ridge, arranging the culture materials into arch-shaped ridges, then pressing the strains into the depth of 2 cm in surface layer materials, carrying out dibbling three rows in sequence along the material ridges, wherein the distance between the blocks is 8-10 cm, covering the used material of the blocks in the holes tightly, paving a small amount of material in a ditch with the distance of 12 cm between the two ridges to the thickness of 3 cm, forming the side surfaces of the two ridges into an inclined slope shape, preventing uprighting, shaping one bed of two ridges, flattening the two ridges, then covering soil, taking the soil on an operation channel, and covering the thickness of the material ridges to be 1.5-2 cm.
Compared with the prior art, the invention has the beneficial effects that: the invention breaks through the technical mode that the traditional culture medium material has single nutrition and the conventional method has long strain preparation period, overcomes the defects of short fruiting period and low yield, adopts liquid strains to prepare culture strains, utilizes the comprehensive culture medium material to culture the stropharia rugoso-annulata, and practices prove that in cold temperate areas, compared with the conventional method for culturing the stropharia rugoso-annulata, the method for culturing the stropharia rugoso-annulata can implement artificial culture 15 days in advance, prolong the fruiting period by about 20 days, and improve the biological conversion rate by 15-20 percent.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention, the stropharia rugoso-annulata comprehensive culture medium comprises a basic culture solution, wherein the basic culture solution is prepared by decocting fresh stropharia rugoso-annulata sporocarp which is 1.5 mass unit of the domestic water drinking standard and is added with 1.0-2.0 mass unit of sucrose, 0.10-0.50 mass unit of protein powder, 0.20-0.30 mass unit of yeast powder, 0.6-0.7 mass unit of soybean meal, 0.05-0.10 mass unit of dipotassium hydrogen phosphate and 0.05-0.10 mass unit of magnesium sulfate, the pH value is adjusted to 6.3-6.8 after the mixture is uniform, and the medium is prepared by sterilizing and cooling and culturing; (the obtained second-level strain of the stropharia rugoso-annulata liquid can be sprayed and inoculated in a field or under a forest).
A stropharia rugoso-annulata cultivation method comprises the following steps:
the first step is as follows: transferring the stropharia rugoso-annulata mother seeds to a test tube containing a PDA culture medium for activation, recovering the activity of hyphae of the stropharia rugoso-annulata mother seeds, and then cutting to obtain original stropharia rugoso-annulata PDA bacterial blocks;
the second step is that: inoculating the PDA bacterial blocks of the stropharia rugoso-annulata stock seeds to a sterilized PDB liquid culture medium, and culturing for 5-7 days in the dark by using a shaking table at the temperature of 22-26 ℃ to obtain primary stropharia rugoso-annulata seeds;
the third step: preparing a second-class liquid seed, namely inoculating the first-class stropharia rugoso-annulata seed into a fermentation tank containing a stropharia rugoso-annulata liquid culture medium, and then culturing the inoculated fermentation tank at 22-26 ℃ for 4-5 days;
the fourth step: preparing solid culture strains, namely inoculating the obtained liquid secondary strains of the stropharia rugoso-annulata to a solid strain culture medium, and culturing for 40 days at the temperature of 22-26 ℃ in a dark place, wherein the solid strain culture medium is prepared from 78% of mushroom bran, 20% of wheat bran, 1% of sucrose and 1% of lime; preparing according to conventional strain production, inoculating liquid strain for 4-5 days, and culturing for 40 days to obtain solid culture strain;
the fifth step: preparing a comprehensive cultivation base material, namely separating and crushing 70% of bag materials of waste fungus bags with mushrooms and ears, cutting forest weeds into 30% of small sections with the length of 5-8cm, mixing the two materials, adjusting the water content to 65-68% and the pH value to 5-7, and then performing 70 ℃ stacking fermentation treatment on the synthetic compost (the comprehensive cultivation base material);
and a sixth step: bed making, material paving, seeding and material paving in the greenhouse: the bed width is 1.3 m.
Making a bed: firstly, digging 3-4 cm deep soil to the ground, taking out the soil, and placing the soil on an operation channel at intervals of a ridge bed for covering the soil. The bed surface is trimmed to form a turtle back shape with a slightly higher middle part, so as to prevent water accumulation at the bottom of the bed. The bed surface is sprayed with lime powder and phoxim, and the sprayed lime bed surface is white. When the water regulation of the culture material reaches 65 percent, the material temperature is reduced to below 25 ℃ and the material spreading is started.
Spreading materials: firstly, the material is paved about 8-10 cm in thickness and 1.2 m in width, then a material bed of 1.2 m is divided into two ridges, the distance between the two ridges is about 12 cm, the south and north ends of the two ridges are sealed by the material, the material feeding amount is increased, the mushroom yield is increased, and the irrigation is facilitated after the two sides of the ridge and the ditch are plugged.
Sowing: breaking strains into blocks with the sizes of peaches, transversely playing three holes in each small single ridge, enabling the distance between the strain blocks to be 8-10 cm, dibbling the strain blocks in three rows in sequence along the ridges, enabling the interval between the strain blocks to be 8-10 cm, paving culture materials with the thickness of 8-10 cm on each single ridge after the first-layer sowing is completed, arranging the culture materials into arch-shaped ridges, then pressing the strains into the depth of 2 cm in surface layer materials, sequentially carrying out dibbling in three rows along the material ridges, enabling the distance between the strain blocks to be 8-10 cm, tightly covering the material used for the strain blocks in the holes by hands or rakes, and paving a small amount of the material with the thickness of 3 cm in a ditch with the distance of 12 cm between two ridges, so that a large amount of mushrooms in the ditch can be produced conveniently. The side surfaces of the two ridges are slope-shaped, so that the two ridges cannot stand on the ground, and the two ridges are prevented from sliding down during soil covering. Shaping the double ridges of one bed, gently patting the double ridges with a wood board, and then covering soil. Taking soil on the operation way, covering the ridge with the thickness of 1.5-2 cm, and enabling the soil to be good in granular shape. And then normally managing.
Claims (7)
1. The stropharia rugoso-annulata comprehensive culture medium is characterized by comprising a basic culture solution, wherein the basic culture solution is prepared by decocting fresh stropharia rugoso-annulata sporocarp which is 1.5 mass unit of the domestic water drinking standard, 1.0-2.0 mass unit of sucrose, 0.10-0.50 mass unit of protein powder, 0.20-0.30 mass unit of yeast powder, 0.6-0.7 mass unit of soybean meal, 0.05-0.10 mass unit of dipotassium hydrogen phosphate and 0.05-0.10 mass unit of magnesium sulfate are added into the basic culture solution, the pH value is adjusted to 6.3-6.8 after the mixture is uniformly mixed, and the culture is carried out after the sterilization and cooling.
2. The stropharia rugoso-annulata cultivation method is characterized by comprising the following steps:
step 1, transferring the stropharia rugoso-annulata mother seeds to a test tube containing a PDA culture medium for activation, recovering the activity of hyphae of the stropharia rugoso-annulata mother seeds, and then cutting to obtain original stropharia rugoso-annulata PDA bacterial blocks;
step 2, inoculating the PDA bacterial blocks of the stropharia rugoso-annulata stock seeds to a sterilized PDB liquid culture medium, and culturing for 5-7 days in the dark by using a shaking table at the temperature of 22-26 ℃ to obtain primary stropharia rugoso-annulata seeds;
step 3, preparing liquid second-level seeds: inoculating the first-class strain of Stropharia rugosoannulata into a fermentation tank containing a comprehensive culture medium of the Stropharia rugosoannulata, and then culturing the inoculated fermentation tank at 22-26 ℃ for 4-5 days;
step 4, preparing solid culture strains;
step 5, preparing a comprehensive cultivation base material;
and 6, making a bed, paving materials, sowing and paving materials in the greenhouse.
3. The stropharia rugoso-annulata cultivation method according to claim 2, characterized in that the step 4 specifically comprises: inoculating the obtained liquid secondary strain of the stropharia rugoso-annulata to a solid strain culture medium, and culturing for 40 days at the temperature of 22-26 ℃ in the dark, wherein the solid strain culture medium is prepared from 78% of mushroom bran, 20% of wheat bran, 1% of cane sugar and 1% of lime; preparing according to conventional strain production, inoculating liquid strain cultured for 4-5 days, and culturing for 40 days to obtain solid culture strain.
4. The stropharia rugoso-annulata cultivation method according to claim 2, characterized in that the step 5 specifically comprises: separating and crushing 70% of bag materials of waste fungus bags with over-mushrooms and ears, cutting forest weeds into small sections with the length of 5-8cm and the length of 30%, mixing the two materials, adjusting the water content to 65-68% and the pH value to 5-7, and then performing 70 ℃ stacking fermentation treatment on the synthesized comprehensive cultivation base material.
5. The stropharia rugoso-annulata cultivation method according to claim 2, characterized in that the concrete steps of making the bed are as follows: the width of the bed is 1.3 meters, the soil is firstly dug to the ground and taken out with the depth of 3-4 centimeters, the soil is placed on an operation way at intervals of the ridge beds for covering soil, the bed surface is trimmed to be a turtle back type with a slightly higher middle part to prevent water accumulation at the bottom of the bed, lime powder and phoxim are sprayed on the bed surface, and when the water regulation of the culture material reaches 65 percent, the material temperature is reduced to be below 25 ℃, the material spreading is started.
6. The stropharia rugoso-annulata cultivation method according to claim 2, characterized in that the concrete steps of paving are as follows: firstly, the material is paved about 8-10 cm in thickness and 1.2 m in width, then a material bed of 1.2 m is divided into two ridges, the distance between the two ridges is about 12 cm, the south and north ends of the two ridges are sealed by the material, the material feeding amount is increased, the mushroom yield is increased, and the irrigation is facilitated after the two sides of the ridge and the ditch are plugged.
7. The stropharia rugoso-annulata cultivation method according to claim 2, characterized in that the specific steps of sowing are as follows: dividing strains into walnut blocks, transversely playing three holes in each small single ridge, wherein the distance between the blocks is 8-10 cm, dibbling three rows in sequence along the ridges, the distance between the blocks is 8-10 cm, after the first-layer sowing is finished, paving culture materials with the thickness of 8-10 cm on each single ridge, arranging the culture materials into arch-shaped ridges, then pressing the strains into the depth of 2 cm in surface layer materials, carrying out dibbling three rows in sequence along the material ridges, wherein the distance between the blocks is 8-10 cm, covering the used material of the blocks in the holes tightly, paving a small amount of material in a ditch with the distance of 12 cm between the two ridges to the thickness of 3 cm, forming the side surfaces of the two ridges into an inclined slope shape, preventing uprighting, shaping one bed of two ridges, flattening the two ridges, then covering soil, taking the soil on an operation channel, and covering the thickness of the material ridges to be 1.5-2 cm.
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| CN116616118A (en) * | 2023-01-10 | 2023-08-22 | 国家林业和草原局竹子研究开发中心 | Method for cultivating stropharia rugoso-annulata by using morchella waste nutrition bags |
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| CN111615996A (en) * | 2020-05-19 | 2020-09-04 | 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心） | Stropharia rugosoannulata strain breeding method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114342734A (en) * | 2021-11-10 | 2022-04-15 | 中国科学院微生物研究所 | Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body |
| CN116616118A (en) * | 2023-01-10 | 2023-08-22 | 国家林业和草原局竹子研究开发中心 | Method for cultivating stropharia rugoso-annulata by using morchella waste nutrition bags |
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