CN114342734A - Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body - Google Patents
Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body Download PDFInfo
- Publication number
- CN114342734A CN114342734A CN202111329002.1A CN202111329002A CN114342734A CN 114342734 A CN114342734 A CN 114342734A CN 202111329002 A CN202111329002 A CN 202111329002A CN 114342734 A CN114342734 A CN 114342734A
- Authority
- CN
- China
- Prior art keywords
- strain
- liquid
- annulata
- stropharia rugoso
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 117
- 241000958510 Stropharia rugosoannulata Species 0.000 title claims abstract description 78
- 230000009467 reduction Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000012364 cultivation method Methods 0.000 title claims description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 45
- 238000000034 method Methods 0.000 claims abstract description 30
- 238000002156 mixing Methods 0.000 claims abstract description 25
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 239000008188 pellet Substances 0.000 claims abstract description 17
- 230000004913 activation Effects 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims description 40
- 241000209094 Oryza Species 0.000 claims description 30
- 235000007164 Oryza sativa Nutrition 0.000 claims description 30
- 235000009566 rice Nutrition 0.000 claims description 30
- 239000010902 straw Substances 0.000 claims description 30
- 241000894007 species Species 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 22
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 22
- 239000010440 gypsum Substances 0.000 claims description 22
- 229910052602 gypsum Inorganic materials 0.000 claims description 22
- 239000004571 lime Substances 0.000 claims description 22
- 235000013312 flour Nutrition 0.000 claims description 18
- 238000009331 sowing Methods 0.000 claims description 16
- 238000009736 wetting Methods 0.000 claims description 16
- 240000008042 Zea mays Species 0.000 claims description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 15
- 235000005822 corn Nutrition 0.000 claims description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 13
- 239000002023 wood Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 11
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 11
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 241000609240 Ambelania acida Species 0.000 claims description 7
- 238000001994 activation Methods 0.000 claims description 7
- 239000010905 bagasse Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000019764 Soybean Meal Nutrition 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 239000004455 soybean meal Substances 0.000 claims description 4
- 229960004793 sucrose Drugs 0.000 claims description 4
- 239000010828 animal waste Substances 0.000 claims description 3
- 239000002518 antifoaming agent Substances 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 9
- 238000011218 seed culture Methods 0.000 description 10
- 244000068988 Glycine max Species 0.000 description 9
- 235000010469 Glycine max Nutrition 0.000 description 9
- 239000002994 raw material Substances 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 238000009630 liquid culture Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000958507 Stropharia Species 0.000 description 3
- 241000123672 Strophariaceae Species 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000222485 Agaricales Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 240000007263 Pinus koraiensis Species 0.000 description 1
- 235000011615 Pinus koraiensis Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 241000121219 Tricholoma Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009342 intercropping Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of edible mushroom cultivation, and relates to a preparation method of a stropharia rugoso-annulata (S.rugosoanulata) liquid reduction strain, which comprises the following steps: (1) activation of stropharia rugoso-annulata strains: inoculating stropharia rugoso-annulata mycelium to a PDA culture medium, and culturing for 10-15 days at 20-28 ℃ to obtain a mother strain; (2) preparing a first-stage liquid strain: transferring the mother strain to a primary liquid strain culture medium, and performing shaking culture at 150-180rpm and 20-28 ℃ in a dark place for 7-10 days to obtain a primary liquid strain; (3) preparing a secondary liquid strain: inoculating the primary liquid strain to a secondary liquid strain culture medium, and fermenting at 20-28 deg.C for 3-5d to obtain a secondary liquid strain; (4) preparing liquid reduction strains: and mixing the mycelium pellet of the secondary liquid strain with a reduction strain culture solution to obtain a liquid reduction strain. The invention also relates to a method for cultivating stropharia rugoso-annulata sporocarp by utilizing the liquid reduction strain. The method can greatly shorten the culture period of the stropharia rugoso-annulata strains.
Description
Technical Field
The invention belongs to the technical field of edible mushroom cultivation, and relates to a preparation method of a liquid reduction strain of stropharia rugoso-annulata and a fruiting body cultivation method thereof.
Background
Stropharia rugosoannulata (Stropharia rugosoannulata farl. ex Murrill) also known as Stropharia rugosoannulata and Cladosporus vinifera, commonly known as Tricholoma rubrum and Pinus koraiensis, belongs to Agaricales in biological classification, Strophariaceae (Strophariaceae), Strophariaceae (Stropharia) and Stropharia (Stropharia) and is crisp and tender in taste and delicious in taste and rich in various nutritional components such as proteins, taurine, flavonoids and the like. The edible fungi are widely traded in the international edible fungi market as the characteristic edible fungi recommended to developing countries by the Food and Agriculture Organization (FAO) of the United nations. The stropharia rugoso-annulata cultivation medium is wide, such as various agricultural and forestry wastes, straws, other edible fungus residues and the like, can be used as a cultivation medium, can be used as a cultivation place indoors, outdoors or in forest lands, and can be used for crop rotation or intercropping planting. In recent years, domestic stropharia rugoso-annulata cultivation develops rapidly, and according to incomplete statistics, the planting area in the country in 2019 exceeds 2 ten thousand mu, which is increased by 1 time compared with nearly 1 ten thousand mu in 2017.
With the increase of planting area, the strain supply demand is continuously increased. The strain of stropharia rugoso-annulata needs to be cultured for 3 months or even longer from the culture of mother seeds to the sowing, and the preparation of liquid reduction strain draws attention in order to save time, raw material cost and facilitate transportation.
The prior art needs a method for preparing liquid reduction strains of stropharia rugoso-annulata and cultivating sporocarp, which obviously shortens the culture period and is convenient to transport.
Disclosure of Invention
In view of the above, the present invention aims to provide a preparation method of a liquid reduction strain of stropharia rugoso-annulata and a method for cultivating a fruit body by using the reduction strain, so as to solve the technical problems of long preparation period and low production benefit of the existing stropharia rugoso-annulata strain.
Therefore, in one aspect, the present invention provides a method for preparing a liquid reduced strain of stropharia rugosoannulata, comprising the steps of:
(1) activation of stropharia rugoso-annulata strains: inoculating stropharia rugoso-annulata mycelium to a PDA culture medium, and culturing for 10-15 days at 20-28 ℃ to obtain a mother strain;
(2) preparing a first-stage liquid strain: transferring the mother strain to a primary liquid strain culture medium, and performing shaking culture at 150-180rpm and 20-28 ℃ in a dark place for 7-10 days to obtain a primary liquid strain;
(3) preparing a secondary liquid strain: inoculating the primary liquid strain to a secondary liquid strain culture medium, and fermenting at 20-28 deg.C for 3-5d to obtain a secondary liquid strain;
(4) preparing liquid reduction strains: and mixing the mycelium pellet of the secondary liquid strain with a reduction strain culture solution to obtain a liquid reduction strain.
In a second aspect, the present invention provides a method for cultivating a fruiting body of Stropharia rugosoannulata using the liquid reducing strain of Stropharia rugosoannulata of the first aspect, comprising the steps of:
(1) preparation of cultivars: inoculating the liquid reduced strain of the first aspect to a cultivar medium, and culturing the cultivar; and
(2) sowing in a field and covering soil: sowing the cultured cultivar in the cultivation material, and culturing in the dark at 20-28 deg.C for 30-40d to obtain Stropharia rugoso-annulata fruiting body.
Compared with the prior art, the invention has beneficial technical effects. Specifically, in the method of the first aspect of the present invention, by improving the components and culture parameters of the primary liquid spawn culture medium, uniform and fine mycelium pellets are obtained, increasing the biomass of the primary liquid spawn, facilitating the expanded culture of the secondary liquid spawn; compared with the traditional methods for preparing stock seeds and cultivated seeds, the culture period of the strains is obviously shortened by culturing the primary liquid strains and the secondary liquid strains; compared with the transportation of cultivation species for cultivating stropharia rugoso-annulata, the liquid reduction strain is prepared, so that the transportation is convenient, the strain is prevented from being damaged in the transportation process, and the production and transportation costs are reduced.
Drawings
FIG. 1 shows the growth of mycelia of Stropharia rugosoannulata (CGMCC No.52220) in PDA plate culture.
FIG. 2 shows the growth of stropharia rugoso-annulata (CGMCC No.52220) inoculated in 3 kinds of first-class liquid culture medium with different formulas, A: the composite culture medium of the invention, B: PDB medium, C: corn flour culture medium.
FIG. 3 shows the secondary liquid spawn growth status of stropharia rugoso-annulata (CGMCC No.52220) strain inoculated composite material culture medium.
FIG. 4 shows the spawn running state of stropharia rugoso-annulata (CGMCC No.52220) after the second-level liquid spawn is inoculated.
FIG. 5 shows the fruiting situation of Stropharia rugosoannulata (CGMCC No.52220) strain in laboratory.
FIG. 6 shows the fruiting of stropharia rugoso-annulata (CGMCC No.52220) in the cultivation material A.
FIG. 7 shows the fruiting of stropharia rugoso-annulata (CGMCC No.52220) in the cultivation material B.
FIG. 8 shows the fruiting of stropharia rugoso-annulata (CGMCC No.52220) in the cultivation material C.
Detailed Description
The inventor surprisingly discovers that the components and the proportion of the primary liquid spawn of the stropharia rugoso-annulata are improved, the culture formula is optimized, uniform and small stropharia rugoso-annulata mycelium pellets are obtained, the biomass of the primary liquid spawn is increased, and the expansion culture of the secondary liquid spawn is facilitated; compared with the traditional seed production method, the method obviously shortens the culture time of the strains and obviously reduces the production cost by culturing the primary liquid strain and the secondary liquid strain.
Accordingly, in a first aspect of the present invention, there is provided a method for preparing a liquid reduced species of stropharia rugosoannulata, the method comprising the steps of:
(1) activation of stropharia rugoso-annulata strains: inoculating stropharia rugoso-annulata mycelium to a PDA culture medium, and culturing for 10-15 days at 20-28 ℃ to obtain a mother strain;
(2) preparing a first-stage liquid strain: transferring the mother strain to a primary liquid strain culture medium, and performing shaking culture at 150-180rpm and 20-28 ℃ in a dark place for 7-10 days to obtain a primary liquid strain;
(3) preparing a secondary liquid strain: inoculating the primary liquid strain to a secondary liquid strain culture medium, and fermenting at 20-28 deg.C for 3-5d to obtain a secondary liquid strain;
(4) preparing liquid reduction strains: and mixing the mycelium pellet of the secondary liquid strain with a reduction strain culture solution to obtain a liquid reduction strain.
Stropharia rugosoannulata strain activation
Stropharia rugosoannulata mycelium can be harvested, for example, by field harvesting, isolation, culture, or can be purchased from commercial sources.
In the activation process of the stropharia rugoso-annulata strain, the culture time can be 10-15d, such as 10d, 13d and 15 d. The culture temperature may be 20 ℃ to 28 ℃, for example 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25, 26 ℃, 27 ℃, 28 ℃, preferably 25 ℃.
Preparation of first-grade liquid strain of stropharia rugoso-annulata
In an embodiment of the first aspect of the present invention, the primary liquid seed culture of Stropharia rugosoannulata is prepared by inoculating the activated Stropharia rugosoannulata mother strain into the primary liquid seed culture medium and culturing at 20-28 ℃ and 150-.
In an embodiment of the first aspect of the invention, the primary liquid seed culture medium comprises, in 1000 mL: 15-30g of glucose, 2.0-5.0g of bran, 1.0-1.5g of monopotassium phosphate, 2.0-5.0g of corn flour and 5.0-8.0g of soybean flour, and adding water to a constant volume of 1000 mL. In particular embodiments of the invention, the glucose may be 15g, 16g, 17g, 18g, 19g, 20g, 21g, 22g, 23g, 24g, 25g, 26g, 27g, 28g, 29g, 30 g. In particular embodiments of the invention, the bran may be 2.0g, 2.5g, 3.0g, 3.5g, 4.0g, 4.5g, 5.0 g. In a specific embodiment of the invention, the potassium dihydrogen phosphate may be 1.0g, 1.1g, 1.2g, 1.3g, 1.4g, 1.5 g. In particular embodiments of the invention, the corn meal may be 2.0g, 2.5g, 3.0g, 3.5g, 4.0g, 4.5g, 5.0 g. In a specific embodiment of the present invention, the soybean flour may be 5.0g, 5.5g, 6.0g, 6.5g, 7.0g, 7.5g, 8.0 g.
In the primary liquid seed culture preparation step, the culture temperature may be 20 ℃ to 28 ℃, for example, 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃, 27 ℃, 28 ℃, preferably 25 ℃, and the culture time may be 7-10d, for example, 7d, 7.5d, 8d, 8.5d, 9d, 9.5d, 10 d.
In the primary liquid seed culture preparation step, the cultivation may be carried out at 150-180rpm, for example, 160rpm, 170rpm, 180 rpm.
Preparation of stropharia rugoso-annulata secondary liquid strain
In an embodiment of the first aspect of the present invention, the secondary liquid seed culture is prepared by inoculating the primary liquid seed culture of Stropharia rugosoannulata to a secondary liquid seed culture medium and fermenting at 20 ℃ to 28 ℃ for 3-5 days.
In an embodiment of the first aspect of the invention, the secondary liquid seed culture medium comprises, in 1000 mL: 15.0-20.0g of glucose, 2.0-5.0g of corn flour, 5.0-8.0g of soybean flour, 2.0-5.0g of bran, 1.0-1.5g of monopotassium phosphate and 0.1-0.3g of defoaming agent, and water is added to the mixture to reach the constant volume of 1000 mL. In particular embodiments of the invention, the glucose may be 15.0g, 15.5g, 16.0g, 16.5g, 17.0g, 17.5g, 18.0g, 18.5g, 19.0g, 19.5g, 20.0 g. In particular embodiments of the invention, the corn meal may be 2.0g, 2.5g, 3.0g, 3.5g, 4.0g, 4.5g, 5.0 g. In a specific embodiment of the present invention, the soybean flour may be 5.0g, 5.5g, 6.0g, 6.5g, 7.0g, 7.5g, 8.0 g. In particular embodiments of the invention, the bran may be 2.0g, 2.5g, 3.0g, 3.5g, 4.0g, 4.5g, 5 g. In a specific embodiment of the invention, the potassium dihydrogen phosphate may be 1.0g, 1.1g, 1.2g, 1.3g, 1.4g, 1.5 g. In particular embodiments of the invention, the defoamer may be 0.1g, 0.15g, 0.2g, 0.25g, 0.3 g.
In the secondary liquid strain preparation process, the fermentation temperature may be 20 deg.C to 28 deg.C, such as 20 deg.C, 22 deg.C, 23 deg.C, 24 deg.C, 25 deg.C, 26 deg.C, 27 deg.C, 28 deg.C, preferably 25 deg.C, and the fermentation time may be 3-5d, such as 3d, 3.5d, 4d, 4.5d, 5 d.
Preparation of stropharia rugoso-annulata liquid reduction strain
In an embodiment of the first aspect of the present invention, the liquid reduced stropharia rugoso-annulata strain is prepared by uniformly mixing the mycelial pellets of stropharia rugoso-annulata produced during the preparation of the second-stage liquid strain of stropharia rugoso-annulata with the reduced strain culture solution.
In an embodiment of the first aspect of the invention, the reduced species broth comprises, in 1000 mL: 10.0-15.0g of cane sugar, 5.0-8.0g of soybean powder, 1.0-1.5g of monopotassium phosphate and 0.5-1.0g of magnesium sulfate, and adding water to a constant volume of 1000 mL. In particular embodiments of the invention, the sucrose may be 10.0g, 10.5g, 11.0g, 11.5g, 12.0g, 12.5g, 13.0g, 13.5g, 14.0g, 14.5g, 15.0 g. In a specific embodiment of the present invention, the soybean flour may be 5.0g, 5.5g, 6.0g, 6.5g, 7.0g, 7.5g, 8.0 g. In a specific embodiment of the invention, the potassium dihydrogen phosphate may be 1.0g, 1.1g, 1.2g, 1.3g, 1.4g, 1.5 g. In a specific embodiment of the invention, the magnesium sulfate may be 0.5g, 0.6g, 0.7g, 0.8g, 0.9g, 1.0 g.
In an embodiment of the first aspect of the present invention, the mycelial pellets of the second liquid species of stropharia rugoso-annulata may be obtained by evacuating the second liquid species of stropharia rugoso-annulata. The mycelial pellets produced by the method of the first aspect of the invention are more uniform and finer than conventional methods, typically having a diameter of less than or equal to 3.0mm, for example a diameter of 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 mm.
In an embodiment of the first aspect of the present invention, the mixing ratio of the mycelial pellets of the second-stage liquid species of stropharia rugoso-annulata to the culture solution of the reducing species may be 1:1 to 1:2, for example, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1: 2.
According to the conventional breeding technique, the species of stropharia rugoso-annulata include mother species, stock species and cultivated species, and the culture period is about 4 months or more, but the time can be greatly shortened to 20-30d by adopting the method of the first aspect of the invention.
In a second aspect of the present invention, there is provided a method for cultivating a fruiting body of Stropharia rugosoannulata using the liquid reducing strain of Stropharia rugosoannulata of the first aspect, comprising the steps of:
(1) preparation of cultivars: inoculating the liquid reduction strain of the stropharia rugoso-annulata prepared in the first aspect of the invention to a culture medium of a cultivated species, and culturing the cultivated species; and
(2) sowing in a field: sowing the cultured cultivar in the cultivation material, and culturing in the dark at 20-28 deg.C for 30-40d to obtain the fruiting body of Stropharia rugosoannulata.
In an embodiment of the second aspect of the invention, the cultivar medium is prepared by: 40% of wood chips, 25% of corncobs, 13% of bran, 20% of rice hulls, 1% of lime and 1% of gypsum, pre-wetting the wood chips, the corncobs and the rice hulls before mixing to enable the water content of the wood chips, the corncobs and the rice hulls to reach 60-65%, then adding the bran, the lime and the gypsum, uniformly stirring, bagging according to the specification of 500 g/bag, and autoclaving for 1.5-2 hours to obtain the culture medium for the cultivars. In a specific embodiment of the invention, the moisture content of the wood chips, corn cobs and rice hulls after sufficient pre-wetting can be 60%, 60.5%, 61%, 61.5%, 62%, 62.5%, 63%, 63.5%, 64%, 64.5%, 65%.
In the process of preparing the cultivar, the liquid reduction strain of stropharia rugoso-annulata prepared by the first aspect of the invention can be diluted by 5-10 times, and then inoculated into a cultivar culture medium through a liquid inoculator, wherein the inoculum size is 15-30ml/500g, and the strain is cultured at 20-28 ℃ in the dark, and the relative humidity of air is about 60-65%.
In the field sowing stage, the cultivation material can be one of the following materials:
and (3) cultivation material A: cultivation material: pre-wetting crop straw 60% and rice hull 20% until the water content reaches 60-65%, and mixing with bran 18%, lime 1% and gypsum 1% uniformly to obtain the product;
and (3) cultivation material B: pre-wetting bagasse 60%, crop straw 28% and rice hull 10% until the water content reaches 60-65%, and uniformly mixing with lime 1% and gypsum 1%; or
And (3) cultivation material C: pre-wetting 40% of wood chips, 20% of crop straws, 20% of corncobs, 15% of rice hulls and 3% of animal wastes until the water content reaches 60-65%, and then uniformly mixing the wet wood chips, the lime 1% and the gypsum 1% for fermentation for 10-15 days.
In a specific embodiment of the present invention, the crop straw may be millet straw, corn straw, rice straw, or other crop straw or a mixture thereof.
In a specific embodiment of the invention, for compost a, the moisture content of the crop straw rice hulls after sufficient pre-wetting can be 60%, 60.5%, 61%, 61.5%, 62%, 62.5%, 63%, 63.5%, 64%, 64.5%, 65%.
In a specific embodiment of the present invention, for compost B, the moisture content of bagasse, crop straw, rice husk after sufficient pre-wetting may be 60%, 60.5%, 61%, 61.5%, 62%, 62.5%, 63%, 63.5%, 64%, 64.5%, 65%.
In a specific embodiment of the present invention, the moisture content of the wood chips, crop straws, corn cobs and rice husks after sufficient pre-wetting can be 60%, 60.5%, 61%, 61.5%, 62%, 62.5%, 63%, 63.5%, 64%, 64.5% and 65% for cultivation material C.
Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and substitutions may be made by those skilled in the art without departing from the spirit and scope of the invention, and all such modifications and substitutions are intended to be within the scope of the claims.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 method for cultivating fruiting body of Stropharia rugoso-annulata (CGMCC No.52220)
First, cultivation process
The cultivation process of the fruiting body of Stropharia rugoso-annulata (CGMCC No.52220) is as follows: mother seed activation → first-stage liquid strain preparation → second-stage liquid strain preparation → inoculation → cultivation seed spawn running management → sowing and earthing-up → fruiting management → fruiting body harvesting.
(1) Activation of mother seeds: inoculating original strain of Stropharia rugoso-annulata (CGMCC No.52220) preserved at-80 deg.C or 4 deg.C to mother culture medium (PDA test tube slant/plate), culturing at 25 deg.C in dark place until the culture medium is full of mycelia, and collecting mycelium as mother culture (FIG. 1).
Mother strain PDA culture medium: slicing peeled potato 200g, boiling in 900mL water for 20-30min, adding agar 20g into the filtrate, heating to dissolve completely, adding water to a constant volume of 1000mL, and sterilizing at 121 deg.C for 30min to obtain 1000mLPDA culture medium.
(2) Preparing a first-stage liquid strain: transferring the mother strain obtained in the step (1) into a primary liquid strain culture medium, wherein the inoculation amount is that 8-10 mother strain blocks with the diameter of 1.0cm are evenly inoculated into the liquid culture medium with the volume of 200mL, and the mother strain blocks are shaken at 150rpm and 25 ℃ in a dark place for 7-10 days to obtain the primary liquid strain.
The primary liquid strain culture medium is a composite culture medium: putting 2g of bran into 900mL of water, boiling for 20min, adding 15g of glucose, 1g of monopotassium phosphate, 2g of corn flour and 5g of soybean flour into the filtrate, heating and stirring uniformly, adding water to a constant volume of 1000mL, and obtaining 1000mL of the composite culture medium;
(3) preparing a secondary liquid strain: transferring the liquid strain obtained in the step (2) into a 5000mL fermentation tank (containing 3000mL of liquid), inoculating 200mL of primary liquid strain, and fermenting at 25 ℃ for 3d to obtain secondary liquid strain. The hyphae are wound into balls during germination and growth by continuous oscillation during liquid culture to form uniform and fine hypha balls (the diameter is about 2-3 mm).
The secondary liquid strain culture medium: 45g of glucose, 6g of corn flour, 15g of soybean flour, 6g of bran, 3g of monopotassium phosphate, 0.3g of defoaming agent and 3000mL of tap water.
(4) Preparing and transporting pure strains: and (4) separating mycelium pellets from the secondary liquid strain obtained in the step (3) by a vacuum filtration method, collecting the mycelium pellets in a sterile self-sealing bag, and storing and transporting the mycelium pellets at 4-10 ℃.
(5) Preparing a reduction strain: uniformly mixing the reduced strain culture solution and the pure strain according to the volume ratio of 1:1, and diluting by 5 times for later use.
The reduction strain culture solution: 10g of cane sugar, 5g of soybean meal, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and 1000mL of tap water.
(6) Liquid reduction strain inoculation and spawn running management: inoculating the liquid reduced strain obtained in the step (5) into a culture medium of a culture strain, wherein the inoculation ratio is 15 mL/bag, the inoculation method comprises the steps of inserting a culture material into a liquid inoculator, quantitatively injecting the liquid strain, and culturing at 20-28 ℃ in a dark place, wherein the relative humidity of air is 60% (figure 4).
The culture material for the cultivated species consists of 40 percent of sawdust, 25 percent of corncob, 20 percent of bran, 13 percent of rice hull, 1 percent of lime and 1 percent of gypsum, and the preparation method comprises the following steps: pre-wetting sawdust, corn cob and rice hull to make water content reach 60-65%, adding bran, lime and gypsum, stirring, bagging according to 500 g/bag, and sterilizing at 121 deg.C for 1.5-2 hr.
(7) Sowing in a field and covering soil: and (6) breaking the cultivated seeds into blocks with the sizes of the eggs after the hyphae grow over the bagged materials, uniformly sowing the blocks on the surface of the cultivated materials, covering a layer of soil on the surface of the cultivated materials, finally covering a layer of straws, culturing the seedlings in a dark place at 25-28 ℃ for 5-7 days, spraying water on the surfaces of the straws, wherein the relative humidity of the air is 70% -75%, and ventilating for 1 time every day to maintain the relative concentration of carbon dioxide in the air to be 0.1% -0.5% (volume fraction).
The cultivation material can be raw material or cooked material cultivation, wherein the formula I of the raw material is as follows: the fertilizer is prepared by mixing 45% of crop straws, 40% of rice hulls, 13% of bran, 1% of lime and 1% of gypsum, pre-wetting the crop straws and the rice hulls before mixing to enable the water content of the crop straws and the rice hulls to reach 60-65%, then adding the bran, the lime and the gypsum, uniformly stirring, paving materials and sowing; the formula II of the raw material is as follows: is prepared by mixing 60 percent of bagasse, 25 percent of crop straws, 13 percent of rice hulls, 1 percent of lime and 1 percent of gypsum, pre-wetting the bagasse, the crop straws and the rice hulls before mixing to ensure that the water content reaches 60 to 65 percent, adding auxiliary materials such as the lime, the gypsum and the like, uniformly stirring, spreading and sowing.
The clinker is prepared by mixing the following cultivation raw materials and then carrying out secondary fermentation, namely, pre-fermentation and post-fermentation: 40% of wood chips, 20% of crop straws, 20% of corncobs, 15% of rice hulls, 3% of animal manure (such as dry cow manure), 1% of lime and 1% of gypsum. The pre-fermentation was performed as follows: pre-wetting sawdust, crop straws, corncobs, rice hulls and cow dung 3-4 days before composting, wherein the moisture content is about 65%, the material pile is suitable for the north-south direction, the width of the material pile is generally 2m, the height of the material pile is 1.5-2 m, monitoring the temperature of the pile body, turning the pile body once when the internal temperature of the pile body begins to fall after reaching more than 60 ℃, and completing the pre-fermentation after 3-4 times of pile turning; the post-fermentation was carried out as follows: fermenting again, controlling the temperature in the pile at 60-62 deg.C for 12-24h, ventilating to reduce the temperature of the pile to about 50 deg.C for about 5d, fermenting for about 6-8d, and cooling to room temperature (25-28 deg.C). The cultivation raw material after the secondary fermentation is dark brown, odorless and sour, straw is elastic, manure and grass are uniform, the material is loose, the water content is 60%, and the pH value is 7.5-8, so that the cultivation material is obtained.
(8) And (3) fruiting management: when the hypha cultured in the step (7) grows to the surface of the soil layer, the air temperature is reduced to about 20 ℃, the illumination intensity is stimulated by weak light of 500-800Lux, the relative air humidity is 75-85%, ventilation is carried out for 2-3 times every day, the relative carbon dioxide concentration is reduced to 0.05-0.15% (volume fraction), and the hypha is cultured for 5-10 days.
(9) Harvesting: and (4) harvesting the first tide of mushrooms when the sporocarp grows to be eighty percent mature and the pileus is not unfolded. And (4) cleaning residual mushroom roots and mushroom holes after picking, supplementing soil, performing mushroom fruiting again after hypha is recovered, and harvesting the next tide of mushrooms.
Second, cultivation method
The cultivation method can be laboratory pot culture and field bed cultivation.
Raw material potting in a laboratory: the pot is 1m long and 45cm wide, and mushrooms are cultivated according to the sowing mode.
The ridge bed cultivation method comprises the following steps: the length of the bed is 7m, the width is 50-60cm, the space between beds is 25-30cm, and the cultivation material is flatly paved on the ground surface according to the specification of the bed for cultivation and fruiting.
Comparative example 1 comparison of first-order liquid seed culture Medium
The purpose of this comparative example is to screen a medium which is suitable for the growth of Stropharia rugosoannulata and can significantly improve the mycelium biomass by matching different components of the primary liquid strain medium, and the specific test scheme is as follows:
inoculating the mother strains of the same batch of stropharia rugoso-annulata (CGMCC No.52220) cultured under the same condition into 500mL conical flasks with different liquid culture media (the formula is shown below), wherein the inoculation amount is that 8-10 mother strain blocks with the diameter of 1.0cm are evenly inoculated into each 200mL of liquid culture media, and the mother strain blocks are subjected to 150rpm dark shake culture at 25 ℃ to observe and count the growth condition of mycelium pellets.
The stock culture media tested were three as follows:
A. complex medium of the invention (example 1)
15g of glucose, 1g of monopotassium phosphate, 2g of corn flour, 5g of soybean flour, 2-5g of bran and 1000mL of tap water.
PDB Medium
Peeled potato 200g, glucose 20g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g, tap water to 1000 mL.
C. Corn flour culture medium
15g of glucose, 1.0g of monopotassium phosphate, 2.0g of corn flour and 1000mL of tap water.
As shown in FIG. 2, the strain of Stropharia rugosoannulata (CGMCC No.52220) can grow to form mycelial pellets in all three media, but the biomass is more in the composite medium (FIG. 2, A) of the invention, and the mycelial pellets are fine and uniform, have thick hyphae and have better growth conditions than those in comparative examples 1 (FIG. 2, B) and 2 (FIG. 2, C).
Third, the mushroom production performance of the strain is reduced
The difference of the cultivation material for stropharia rugoso-annulata in the embodiment 1 of the invention is that the fruiting yield of the liquid reduction strain of stropharia rugoso-annulata is compared through different preparation methods of comparative examples 1-3, and the specific implementation scheme is as follows:
inoculating the liquid reduced strain into the culture medium of the cultivated species, inoculating 15 mL/bag, culturing at 25 deg.C in dark, and air relative humidity of 60% (FIG. 4). And (5) preparing for sowing when the hypha grows over the fungus bags.
The cultivation materials for the test field cultivation are three types as follows:
cultivation material 1: 45% of crop straws, 40% of rice hulls, 13% of bran, 1% of lime and 1% of gypsum are mixed, the crop straws and the rice hulls are pre-wetted to reach the water content of 60% before mixing, the lime and the gypsum are added, the mixture is uniformly stirred, and the mixture is spread and sown (figure 6).
And (3) cultivation material 2: is prepared by mixing 60% of bagasse, 25% of crop straw, 13% of rice hull, 1% of lime and 1% of gypsum, pre-wetting the bagasse, the crop straw and the rice hull to the water content of 60% before mixing, adding auxiliary materials such as the lime, the gypsum and the like, uniformly stirring, spreading and sowing (figure 7).
And (3) cultivation material: obtaining the clinker for field cultivation by secondary fermentation of the following cultivation raw materials: is prepared by mixing 40% of wood chips, 20% of crop straws, 20% of corncobs, 15% of rice hulls, 3% of animal wastes (such as dry cow dung), 1% of lime and 1% of gypsum (figure 8).
The results show that the cultivation materials of three different treatments can be used for cultivating stropharia rugoso-annulata by using the strain prepared by the invention, and the formed fruiting bodies are thick in stipe and strong in meat quality, as shown in figures 6, 7 and 8.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same. While the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: modifications may be made to the above-described embodiments, or equivalents may be substituted for some or all of the features thereof without departing from the concept, spirit and scope of the present invention; and such modifications or substitutions are intended to fall within the scope of the invention as claimed.
Claims (8)
1. A method for preparing a liquid reduced strain of stropharia rugosoannulata (s.rugosoanulata), comprising the steps of:
(1) activation of stropharia rugoso-annulata strains: inoculating stropharia rugoso-annulata mycelium to a PDA culture medium, and culturing for 10-15 days at 20-28 ℃ to obtain a mother strain;
(2) preparing a first-stage liquid strain: transferring the mother strain to a primary liquid strain culture medium, and performing shaking culture at 150-180rpm and 20-28 ℃ in a dark place for 7-10 days to obtain a primary liquid strain;
(3) preparing a secondary liquid strain: inoculating the primary liquid strain to a secondary liquid strain culture medium, and fermenting at 20-28 deg.C for 3-5d to obtain a secondary liquid strain;
(4) preparing liquid reduction strains: and mixing the mycelium pellet of the secondary liquid strain with a reduction strain culture solution to obtain a liquid reduction strain.
2. The method for preparing a liquid reduced species of stropharia rugoso-annulata according to claim 1, wherein the primary liquid species culture medium comprises, in 1000 mL: 15-30g of glucose, 2.0-5.0g of bran, 2.0-5.0g of corn flour, 5.0-8.0g of soybean meal and 1.0-1.5g of monopotassium phosphate, and adding water to a constant volume of 1000 mL.
3. The method for preparing a liquid reduced species of stropharia rugoso-annulata according to claim 1, wherein the secondary liquid species culture medium comprises, in 1000 mL: 15-20g of glucose, 2.0-5.0g of bran, 2.0-5.0g of corn flour, 5.0-8.0g of soybean meal, 1.0-1.5g of monopotassium phosphate and 0.1-0.3g of defoaming agent, and adding water to a constant volume of 1000 mL.
4. The method for preparing a liquid reduced species of stropharia rugoso-annulata according to claim 1, wherein the reduced species culture solution comprises, in 1000 mL: 10-15g of cane sugar, 5.0-8.0g of soybean meal, 1.0-1.5g of monopotassium phosphate and 0.5-1.0g of magnesium sulfate, and adding water to a constant volume of 1000 mL.
5. The method for preparing a liquid reduced species of Stropharia rugosoannulata according to claim 1, wherein the mixing ratio of the mycelium pellet of the secondary liquid species to the culture solution of the reduced species is 1:1 to 1: 2.
6. The stropharia rugoso-annulata fruiting body cultivation method is characterized by comprising the following steps:
(1) preparation of cultivars: inoculating the liquid reduced strain of claim 1-5 into a culture medium of a culture, and culturing the culture; and
(2) sowing in a field: sowing the cultured cultivar in the cultivation material, and culturing in the dark at 20-28 deg.C for 30-40d to obtain Stropharia rugoso-annulata fruiting body.
7. The method for cultivating a fruiting body of Stropharia rugoso-annulata according to claim 6, wherein the cultivar medium is prepared by: 40% of wood chips, 25% of corncobs, 13% of bran, 20% of rice hulls, 1% of lime and 1% of gypsum are mixed, the wood chips, the corncobs and the rice hulls are pre-wetted until the water content reaches 60-65% before mixing, then the bran, the lime and the gypsum are added, stirring and bagging are carried out, and autoclaving is carried out for 1.5-2.0h, so as to obtain the culture medium for the cultivated species.
8. The method for cultivating fruit bodies of Stropharia rugoso-annulata according to claim 6, wherein the cultivation material is selected from the group consisting of:
A. cultivation material: pre-wetting crop straw 60% and rice hull 20% until the water content reaches 60-65%, and mixing with bran 18%, lime 1% and gypsum 1% uniformly to obtain the product;
B. cultivation material: pre-wetting bagasse 60%, crop straw 28% and rice hull 10% until the water content reaches 60%, and then uniformly mixing with lime 1% and gypsum 1%; or
C. Cultivation material: pre-wetting 40% of wood chips, 20% of crop straws, 20% of corncobs, 15% of rice hulls and 3% of animal wastes until the water content reaches 60%, and then uniformly mixing the wet wood chips, the lime 1% and the gypsum 1% for fermentation for 10-15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111329002.1A CN114342734A (en) | 2021-11-10 | 2021-11-10 | Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111329002.1A CN114342734A (en) | 2021-11-10 | 2021-11-10 | Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114342734A true CN114342734A (en) | 2022-04-15 |
Family
ID=81095968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111329002.1A Pending CN114342734A (en) | 2021-11-10 | 2021-11-10 | Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114342734A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766284A (en) * | 2022-04-27 | 2022-07-22 | 成都市农林科学院 | Stropharia rugosoannulata high-yield cultivation formula and application thereof |
| CN115316193A (en) * | 2022-05-11 | 2022-11-11 | 山东昌龙农业科技有限公司 | Method for preparing stropharia rugoso-annulata cultivation material by using spearmint straws and broussonetia papyrifera stems and leaves |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228830A (en) * | 2008-02-22 | 2008-07-30 | 江南大学 | Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn |
| CN101699969A (en) * | 2009-11-05 | 2010-05-05 | 张纪明 | Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof |
| CN107455141A (en) * | 2017-07-20 | 2017-12-12 | 南阳市农业科学院 | A kind of cultural method of Stropharia rugoso-annulata |
| CN109234169A (en) * | 2018-08-17 | 2019-01-18 | 蔡树威 | A kind of production method of bolete liquid spawn |
| CN109220559A (en) * | 2018-09-30 | 2019-01-18 | 上海市农业科学院 | A kind of production method and growth condition of Stropharia rugoso-annulata reduction strain |
| CN111903432A (en) * | 2020-08-06 | 2020-11-10 | 贵州珍稀食用菌科技有限公司 | Dictyophora rubrovalvata strain rapid propagation method and application thereof |
| CN113348968A (en) * | 2021-07-13 | 2021-09-07 | 石忠斌 | Preparation of stropharia rugoso-annulata comprehensive culture medium and stropharia rugoso-annulata cultivation method |
-
2021
- 2021-11-10 CN CN202111329002.1A patent/CN114342734A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228830A (en) * | 2008-02-22 | 2008-07-30 | 江南大学 | Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn |
| CN101699969A (en) * | 2009-11-05 | 2010-05-05 | 张纪明 | Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof |
| CN107455141A (en) * | 2017-07-20 | 2017-12-12 | 南阳市农业科学院 | A kind of cultural method of Stropharia rugoso-annulata |
| CN109234169A (en) * | 2018-08-17 | 2019-01-18 | 蔡树威 | A kind of production method of bolete liquid spawn |
| CN109220559A (en) * | 2018-09-30 | 2019-01-18 | 上海市农业科学院 | A kind of production method and growth condition of Stropharia rugoso-annulata reduction strain |
| CN111903432A (en) * | 2020-08-06 | 2020-11-10 | 贵州珍稀食用菌科技有限公司 | Dictyophora rubrovalvata strain rapid propagation method and application thereof |
| CN113348968A (en) * | 2021-07-13 | 2021-09-07 | 石忠斌 | Preparation of stropharia rugoso-annulata comprehensive culture medium and stropharia rugoso-annulata cultivation method |
Non-Patent Citations (4)
| Title |
|---|
| 丁亚通等: "我国中原地区大棚栽培大球盖菇技术", 《食药用菌》 * |
| 于延申等: "大球盖菇菌种生产技术", 《吉林蔬菜》 * |
| 刘金力等: "大球盖菇液体菌种培养基碳氮源及培养条件的优化", 《黑龙江农业科学》 * |
| 龚赛等: "不同栽培条件下大球盖菇的经济效益", 《中国食用菌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766284A (en) * | 2022-04-27 | 2022-07-22 | 成都市农林科学院 | Stropharia rugosoannulata high-yield cultivation formula and application thereof |
| CN115316193A (en) * | 2022-05-11 | 2022-11-11 | 山东昌龙农业科技有限公司 | Method for preparing stropharia rugoso-annulata cultivation material by using spearmint straws and broussonetia papyrifera stems and leaves |
| CN115316193B (en) * | 2022-05-11 | 2023-08-18 | 山东昌龙农业科技有限公司 | Method for preparing stropharia rugoso-annulata cultivation material by using spearmint straw and broussonetia papyrifera stems and leaves |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101696390B (en) | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof | |
| CN105900692B (en) | Method for cultivating hericium erinaceus by using corncobs | |
| CN103396954B (en) | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer | |
| CN106187515B (en) | Utilize the hickory chick nutrient bag and preparation method thereof of edible fungi residue production | |
| CN103382134A (en) | Fermentation method of Agaricus bisporus (Lange) Sing cultivation matrix | |
| CN103382139A (en) | Agaricus bisporus (Lange) Sing culture medium | |
| CN114342734A (en) | Preparation of liquid reduction strain of stropharia rugoso-annulata and cultivation method of fruiting body | |
| CN104641942A (en) | Method for cultivating oyster mushroom on mulberry twigs | |
| CN1212292C (en) | Microorganic fertilizer against various bacteria and its preparation and use | |
| CN108863550A (en) | A kind of dedicated disease prevention growth-promoting composite microbic bacterial fertilizer of tea tree and preparation method thereof | |
| CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
| CN112243815A (en) | Ecological planting method of rice | |
| CN111357612B (en) | Composite microbial matrix for watermelon planting and preparation method and application thereof | |
| CN112369276A (en) | Culture medium for cultivating stropharia rugoso-annulata by using pleurotus eryngii dregs as well as preparation method and application of culture medium | |
| CN106495808A (en) | Potato base manure and potato base manure preparation method | |
| CN108990702B (en) | Facility cultivation method for crop rotation of stropharia rugoso-annulata and cowpea | |
| CN104844285B (en) | A kind of preparation method for the biological organic fertilizer improving cherry and tomato immunity | |
| CN106977245A (en) | A kind of compost of selenium enriched oyster mushroom and the cultural method of selenium enriched oyster mushroom | |
| CN116439071A (en) | Wild-imitating tricholoma matsutake planting technology under forest | |
| CN108770593B (en) | Lepista nuda strain and fruiting body cultivation method thereof | |
| CN110583367A (en) | Edible mushroom production process method | |
| CN110592052A (en) | Production method and application of lumbruse polypeptide antibacterial nutrient solution | |
| JPH11255572A (en) | Material for applying microorganism | |
| JP6967315B1 (en) | Soil improvement materials and compost manufacturing methods and soil improvement materials and compost | |
| CN114946531A (en) | Lepista sordida cultivation nutrient solution and Lepista sordida cultivation method using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220415 |
|
| RJ01 | Rejection of invention patent application after publication |