CN102523918A - Gastrodia elata high-yield planting method by indoor microculture - Google Patents
Gastrodia elata high-yield planting method by indoor microculture Download PDFInfo
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- CN102523918A CN102523918A CN2011104492181A CN201110449218A CN102523918A CN 102523918 A CN102523918 A CN 102523918A CN 2011104492181 A CN2011104492181 A CN 2011104492181A CN 201110449218 A CN201110449218 A CN 201110449218A CN 102523918 A CN102523918 A CN 102523918A
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Abstract
A Gastrodia elata high-yield planting method by indoor microculture relates to Gastrodia elata planting techniques and is characterized by including sequential steps: firstly, clearing a culture room, fumigating to disinfect the culture room with sulfur, and allowing the culture room to stand by; and secondly, sectioning a bacterial material into small cut wood 15cm long, bagging the small wood blocks with strain bags of a 17cmX33cmX0.045cm specification, allowing a plurality of thin wood blocks to be bagged into one bag, breaking thick wood blocks to facilitate bagging, confirming fullness of each bag, using stuffing mix to stuff the uncompacted bags, tying the bags, disinfecting, cooling, inoculating, and performing indoor microculture. The Gastrodia elata high-yield planting method by indoor microculture has the advantages that indoor microculture allows observation of growth and development of hyphae anywhere at any time, hypha growth factors such as temperature, water, luminance and the like can be adjusted timely to affect growth and development of the hyphae, polluted strain bags are cleared timely, secondary inoculation is performed to the bags with no spawn germination, tying ropes at openings of the bags with slow spawning germination can be loosened to oxygenate and promote germination, and accordingly culture success rate of the hyphae on the bacterial material is increased.
Description
Technical field
The present invention relates to the rhizoma Gastrodiae planting technology, be specifically related to a kind of method of culturing fungi and planting tall gastrodia tuber indoors high yield.
Background technology
China's rural area rhizoma Gastrodiae plantation bacterium material cultural hypha stage, process of growth can't be observed all outdoor underground carrying out, and temperature, moisture, illumination etc. influence the mycelial growth factor and is difficult to control, so bacterium material cultural hypha success rate is not high, causes the wasting of resources.
Summary of the invention
The present invention provides a kind of method of culturing fungi and planting tall gastrodia tuber indoors high yield; The grower can observe the hyphal development situation whenever and wherever possible; Influence growing of mycelia through mycelial growth factors such as timely adjustment temperature, moisture, illumination, thereby improve the success rate of bacterium material cultural hypha.
The technical scheme that the present invention adopts is: a kind of method of culturing fungi and planting tall gastrodia tuber indoors is characterized in that carrying out according to the following steps successively:
1, the bacteria chamber clean is clean also with the fumigation of sulphur semi-annular jade pendant, subsequent use;
2, the bacterium material is divided into 15cm segment wood, in the strain bag of 17 * 33 * 0.045cm that packs into then, thin segment wood can many in the bag of packing into simultaneously; Thick section wood can decompose, and in order to pack, sack is filled; Tamp with mixed fillers in unreal place, pricks bag, sterilization then; Cooling, inoculation, indoor bacteria.
Said indoor bacteria is that strain bag is put into bacteria is indoor, and culture period is 65-75 days in the bacteria chamber, and the bacteria indoor air temperature remains between 22 ℃ one 26 ℃, treats to change over to when mycelia is covered with in the strain bag the outdoor bag cultivating that takes off.
The weight proportion of described mixed fillers raw material components is: sawdust 90-110, and wheat skin 50-70, corn flour 15-25, brown sugar 1-3, land plaster 1-3 evenly mixes above-mentioned raw material by the afore mentioned rules proportioning, makes mixed fillers.
Said bacteria chamber require to ventilate, dry, insulation, and is in order to avoid living contaminants when cultivating, will be with the bacteria chamber clean before strain bag gets into the bacteria chamber clean and with the fumigation of sulphur semi-annular jade pendant; In the strain bag entering bacteria chamber 15 days; Personnel enter the bacteria chamber less, try not to stir strain bag, begin to combine turning to check that stage by stage strain bag sends out the bacterium situation about 20 days; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation.If temperature is too high through ventilation, evacuation, turning heat radiation during bacteria in the bag, crosses when slow when discovery has strain bag to send out bacterium, can become flexible the sack jag, urgees bacterium with oxygenation and sends out soon.About culture period 70 days, indoor air temperature remains between 22 ℃ one 26 ℃, treats that the strain bag mycelia changes the outdoor bag cultivating that takes off over to when covering with.
Further:
The weight proportion of said each raw material components of mixed fillers is: sawdust 100, wheat skin 60, corn flour 20, brown sugar 2, land plaster 2.
Said strain bag is 70 days at the indoor culture period of bacteria.
The invention has the beneficial effects as follows: indoor bacteria can be observed the mycelial growth developmental state whenever and wherever possible; In time the factor through mycelial growths such as adjustment temperature, moisture, illumination influences growing of mycelia; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation, and it is slow excessively to send out bacterium; Can become flexible the sack jag and send out soon with the short bacterium of oxygenation, thus the success rate of raising bacterium material cultural hypha.
Embodiment
A kind of method of culturing fungi and planting tall gastrodia tuber indoors high yield: 1) the bacterium material is divided into 15cm segment wood, in the strain bag of 17 * 33 * 0.045cm that packs into then, thin segment wood can many in the bag of packing into simultaneously; Thick section wood can decompose, and in order to pack, sack is filled; Tamp with mixed fillers in unreal place, pricks bag, sterilization then; Cooling, inoculation, indoor bacteria.2) change postvaccinal strain bag over to bacteria chamber discharging, two row's strain bag heads suffer head and are a pile, pile highly 1 one 1.2 meters, and heap is asked and stayed 0.6 1 0.8 meters operation roads.The bacteria chamber require to ventilate, dry, insulation, and is in order to avoid living contaminants when cultivating, will be with the bacteria chamber clean before strain bag gets into the bacteria chamber clean and with the fumigation of sulphur semi-annular jade pendant; In the strain bag entering bacteria chamber 15 days; Personnel enter the bacteria chamber less, try not to stir strain bag, and beginning combines turning inspection strain bag to send out the bacterium situation stage by stage about 20 days; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation.If temperature is too high through ventilation, evacuation, turning heat radiation during bacteria in the bag, crosses when slow when discovery has strain bag to send out bacterium, can become flexible the sack jag, urgees bacterium with oxygenation and sends out soon.About culture period 70 days, indoor air temperature remains between 22 ℃ one 26 ℃, treats that the strain bag mycelia changes the outdoor bag cultivating that takes off over to when covering with.3) described mixed fillers is made up of saw foam, wheat skin, corn flour, brown sugar, land plaster, and each component ratio is 100: 60: 20: 2: 2.
Claims (6)
1. the method for a culturing fungi and planting tall gastrodia tuber indoors high yield, characteristic is to carry out according to the following steps successively: 1) the bacteria chamber clean is clean and with the fumigation of sulphur semi-annular jade pendant, subsequent use; 2) the bacterium material is divided into 15cm segment wood, in the strain bag of 17 * 33 * 0.045cm that packs into then, thin segment wood can many in the bag of packing into simultaneously; Thick section wood can decompose, and in order to pack, sack is filled; Tamp with mixed fillers in unreal place, pricks bag, sterilization then; Cooling, inoculation, indoor bacteria.
2. the method for a kind of culturing fungi and planting tall gastrodia tuber indoors high yield according to claim 1; It is characterized in that said indoor bacteria is that strain bag is put into bacteria is indoor; Culture period is 65-75 days in the bacteria chamber; The bacteria indoor air temperature remains between 22 ℃ one 26 ℃, treats to change over to when mycelia is covered with in the strain bag the outdoor bag cultivating that takes off.
3. the method for a kind of culturing fungi and planting tall gastrodia tuber indoors high yield according to claim 1 is characterized in that the weight proportion of described mixed fillers raw material components is: sawdust 90-110, wheat skin 50-70, corn flour 15-25, brown sugar 1-3, land plaster 1-3.
4. the method for a kind of culturing fungi and planting tall gastrodia tuber indoors high yield according to claim 1 is characterized in that said bacteria chamber requires ventilation, dry, insulation, and is before strain bag gets into the bacteria chamber that the bacteria chamber clean is clean also with the fumigation of sulphur semi-annular jade pendant; In the strain bag entering bacteria chamber 15 days; Personnel enter the bacteria chamber less, try not to stir strain bag, begin to combine turning to check that stage by stage strain bag sends out the bacterium situation about 20 days; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation.
5. the method for a kind of culturing fungi and planting tall gastrodia tuber indoors high yield according to claim 1 is characterized in that the weight proportion of said each raw material components of mixed fillers is: sawdust 100, wheat skin 60, corn flour 20, brown sugar 2, land plaster 2.
6. the method for a kind of culturing fungi and planting tall gastrodia tuber indoors high yield according to claim 1 is characterized in that said strain bag is 70 days at the indoor culture period of bacteria.
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CN2011104492181A CN102523918A (en) | 2011-12-29 | 2011-12-29 | Gastrodia elata high-yield planting method by indoor microculture |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103004469A (en) * | 2012-12-14 | 2013-04-03 | 乐山市金口河区森宝野生植物开发有限公司 | Tall gastrodia tuber culture method, tall gastrodia tuber liquor and tall gastrodia tuber production process by using small-caliber container |
CN104221645A (en) * | 2013-09-22 | 2014-12-24 | 徐大东 | Efficient and energy-saving planting method for tall gastrodia tubers |
CN104871804A (en) * | 2015-06-19 | 2015-09-02 | 习水县龙洋生态食品开发有限公司 | Indoor spawn cultivating and breeding method for gastrodia elata |
CN105165625A (en) * | 2015-10-14 | 2015-12-23 | 成都高山乌天麻种植有限公司 | Tissue culture type gastrodia elata seed breeding technology |
-
2011
- 2011-12-29 CN CN2011104492181A patent/CN102523918A/en active Pending
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103004469A (en) * | 2012-12-14 | 2013-04-03 | 乐山市金口河区森宝野生植物开发有限公司 | Tall gastrodia tuber culture method, tall gastrodia tuber liquor and tall gastrodia tuber production process by using small-caliber container |
CN103004469B (en) * | 2012-12-14 | 2015-01-07 | 乐山市金口河区森宝野生植物开发有限公司 | Tall gastrodia tuber culture method, tall gastrodia tuber liquor and tall gastrodia tuber production process by using small-caliber container |
CN104221645A (en) * | 2013-09-22 | 2014-12-24 | 徐大东 | Efficient and energy-saving planting method for tall gastrodia tubers |
CN104871804A (en) * | 2015-06-19 | 2015-09-02 | 习水县龙洋生态食品开发有限公司 | Indoor spawn cultivating and breeding method for gastrodia elata |
CN105165625A (en) * | 2015-10-14 | 2015-12-23 | 成都高山乌天麻种植有限公司 | Tissue culture type gastrodia elata seed breeding technology |
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Application publication date: 20120704 |