CN101855988B - Method for culturing fungi and planting tall gastrodia tuber indoors - Google Patents

Method for culturing fungi and planting tall gastrodia tuber indoors Download PDF

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Publication number
CN101855988B
CN101855988B CN2010102039718A CN201010203971A CN101855988B CN 101855988 B CN101855988 B CN 101855988B CN 2010102039718 A CN2010102039718 A CN 2010102039718A CN 201010203971 A CN201010203971 A CN 201010203971A CN 101855988 B CN101855988 B CN 101855988B
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bag
strain
strain bag
hypha
fungi
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CN2010102039718A
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CN101855988A (en
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刘�文
秦绍新
陈恩合
吴迪
杜方平
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刘�文
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    • Y02P60/216

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Abstract

The invention discloses a method for culturing fungi and planting tall gastrodia tuber indoors, which comprises the following steps of: firstly, taking raw materials comprising 90 to 110 parts of saw dust, 50 to 70 parts of wheat bran, 15 to 25 parts of corn flour, 1 to 3 parts of brown sugar and 1 to 3 parts of gypsum powder, and uniformly mixing the raw materials to obtain a mixed filler; and cleaning a fungi culture chamber, and fumigating and sterilizing the fungi culture chamber by using sulfur; partitioning a fungi material into small logs of 15 centimeters, binding the small logs and filling the small logs into a strain bag, tamping gaps in the strain bag by using the prepared mixed filler, then binding the bag mouth, performing sterilization, cooling and inoculation, and finally putting the strain bag into the fungi culture chamber. The growth and development states of hypha can be observed at any time and any place by indoor fungi culture, the growth and the development of the hypha can be affected by regulating factors such as temperature, moisture, illumination and the like for the growth of the hypha in time, the polluted strain bags are cleaned in time, the strain bags which do not produce the hypha can be used for secondary inoculation, and the bag mouths of the strain bags which produce the hypha slowly can be loosened to increase oxygen and promote the growth of the hypha, thus, improving the success rate of the fungi hypha.

Description

A kind of method of culturing fungi and planting tall gastrodia tuber indoors
Technical field:
The present invention relates to the rhizoma Gastrodiae planting technology, be specifically related to a kind of method of culturing fungi and planting tall gastrodia tuber indoors.
Background technology:
China's rural area rhizoma Gastrodiae plantation bacterium material cultural hypha stage, process of growth can't be observed all outdoor underground carrying out, and temperature, moisture, illumination etc. influence the mycelial growth factor and is difficult to control, so bacterium material cultural hypha success rate is not high, causes the wasting of resources.
Summary of the invention:
The present invention provides a kind of method of culturing fungi and planting tall gastrodia tuber indoors; The grower can observe the hyphal development situation whenever and wherever possible; Influence growing of mycelia through mycelial growth factors such as timely adjustment temperature, moisture, illumination, thereby improve the success rate of bacterium material cultural hypha.
The technical scheme that the present invention adopts is:
The method of culturing fungi and planting tall gastrodia tuber indoors is characterized in that: carry out according to the following steps successively:
1), the configuration mixed fillers, the weight proportion of said each raw material components of mixed fillers is:
Sawdust 90-110
Wheat skin 50-70
Corn flour 15-25
Brown sugar 1-3
Land plaster 1-3
Above-mentioned raw material are evenly mixed by the afore mentioned rules proportioning, make mixed fillers, subsequent use;
2), the bacteria chamber clean is clean also with the fumigation of sulphur semi-annular jade pendant, subsequent use;
3), the bacterium material is divided into 15cm segment wood, segment wood is tied up in the strain bag of packing into, the space in the strain bag is tamped, tighten sack then with the mixed fillers that makes, sterilization, cooling, inoculation, it is indoor at last strain bag to be put into bacteria; Culture period is 65-75 days in the bacteria chamber, and the bacteria indoor air temperature remains between 22 ℃-26 ℃, treats to change over to when mycelia is covered with in the strain bag the outdoor bag cultivating that takes off.
The method of described culturing fungi and planting tall gastrodia tuber indoors is characterized in that:
The weight proportion of said each raw material components of mixed fillers is:
Sawdust 100
Wheat skin 60
Corn flour 20
Brown sugar 2
Land plaster 2.
The method of described culturing fungi and planting tall gastrodia tuber indoors; It is characterized in that: said strain bag is 70 days at the indoor culture period of bacteria, in 15 days, does not stir strain bag; In 20 days; Check stage by stage that in conjunction with turning strain bag sends out the bacterium situation, contaminated strain bag is cleared up, do not send out the strain bag of bacterium and carry out secondary inoculation.
The grower is divided into 15cm segment wood with the bacterium material, and with in its suitably big or small strain bag of packing into.Thin segment wood can many in the bag of packing into simultaneously, and thick section wood can decompose, and in order to pack, sack is filled, and unreal local available prescription saw foam tamps, and pricks bag then, sterilization, cooling, inoculation, indoor bacteria.
Said bacteria chamber require to ventilate, dry, insulation, and is in order to avoid living contaminants when cultivating, will be with the bacteria chamber clean before strain bag gets into the bacteria chamber clean and with the fumigation of sulphur semi-annular jade pendant; In the strain bag entering bacteria chamber 15 days; Personnel enter the bacteria chamber less, try not to stir strain bag, begin to combine turning to check that stage by stage strain bag sends out the bacterium situation about 20 days; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation.If temperature is too high through ventilation, evacuation, turning heat radiation during bacteria in the bag, crosses when slow when discovery has strain bag to send out bacterium, can become flexible the sack jag, urgees bacterium with oxygenation and sends out soon.About culture period 70 days, indoor air temperature remains on 22 ℃--between 26 ℃, treat that the strain bag mycelia changes the outdoor bag cultivating that takes off over to when covering with.
Advantage of the present invention:
Indoor bacteria can be observed the mycelial growth developmental state whenever and wherever possible; In time the factor through mycelial growths such as adjustment temperature, moisture, illumination influences growing of mycelia; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation, and it is slow excessively to send out bacterium; Can become flexible the sack jag and send out soon with the short bacterium of oxygenation, thus the success rate of raising bacterium material cultural hypha.
Embodiment:
The method of culturing fungi and planting tall gastrodia tuber indoors:
1) the bacterium material is divided into 15cm segment wood, in the strain bag of 17 * 33 * 0.0045cm that packs into then, thin segment wood can many in the bag of packing into simultaneously; Thick section wood can decompose, and in order to pack, sack is filled; Tamp with mixed fillers in unreal place, pricks bag, sterilization then; Cooling, inoculation, indoor bacteria.
2) change postvaccinal strain bag the discharging of over to bacteria chamber, two row's strain bag heads suffer head and for a pile, pile high 1-1.2 rice, stay 0.6-0.8 rice operation road between heap.The bacteria chamber require to ventilate, dry, insulation, and is in order to avoid living contaminants when cultivating, will be with the bacteria chamber clean before strain bag gets into the bacteria chamber clean and with the fumigation of sulphur semi-annular jade pendant; In the strain bag entering bacteria chamber 15 days; Personnel enter the bacteria chamber less, try not to stir strain bag, and beginning combines turning inspection strain bag to send out the bacterium situation stage by stage about 20 days; In time clear up polluting strain bag, that does not send out bacterium can secondary inoculation.If temperature is too high through ventilation, evacuation, turning heat radiation during bacteria in the bag, crosses when slow when discovery has strain bag to send out bacterium, can become flexible the sack jag, urgees bacterium with oxygenation and sends out soon.About culture period 70 days, indoor air temperature remains between 22 ℃-26 ℃, treats that the strain bag mycelia changes the outdoor bag cultivating that takes off over to when covering with.
3) described mixed fillers is made up of saw foam, wheat skin, corn flour, brown sugar, land plaster, and each component ratio is 100: 60: 20: 2: 2.

Claims (3)

1. the method for culturing fungi and planting tall gastrodia tuber indoors is characterized in that: carry out according to the following steps successively:
1), the configuration mixed fillers, the weight proportion of said each raw material components of mixed fillers is:
Sawdust 90-110
Wheat skin 50-70
Corn flour 15-25
Brown sugar 1-3
Land plaster 1-3
Above-mentioned raw material are evenly mixed by the afore mentioned rules proportioning, make mixed fillers, subsequent use;
2), the bacteria chamber clean is clean also with the fumigation of sulphur semi-annular jade pendant, subsequent use;
3), the bacterium material is divided into 15cm segment wood, segment wood is tied up in the strain bag of packing into, the space in the strain bag is tamped, tighten sack then with the mixed fillers that makes, sterilization, cooling, inoculation, it is indoor at last strain bag to be put into bacteria; Culture period is 65-75 days in the bacteria chamber, and the bacteria indoor air temperature remains between 22 ℃-26 ℃, treats to change over to when mycelia is covered with in the strain bag the outdoor bag cultivating that takes off.
2. the method for culturing fungi and planting tall gastrodia tuber indoors according to claim 1 is characterized in that:
The weight proportion of said each raw material components of mixed fillers is:
Sawdust 100
Wheat skin 60
Corn flour 20
Brown sugar 2
Land plaster 2.
3. the method for culturing fungi and planting tall gastrodia tuber indoors according to claim 1; It is characterized in that: said strain bag is 70 days at the indoor culture period of bacteria, in 15 days, does not stir strain bag; In 20 days; Check stage by stage that in conjunction with turning strain bag sends out the bacterium situation, contaminated strain bag is cleared up, do not send out the strain bag of bacterium and carry out secondary inoculation.
CN2010102039718A 2010-06-17 2010-06-17 Method for culturing fungi and planting tall gastrodia tuber indoors Expired - Fee Related CN101855988B (en)

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CN2010102039718A CN101855988B (en) 2010-06-17 2010-06-17 Method for culturing fungi and planting tall gastrodia tuber indoors

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Application Number Priority Date Filing Date Title
CN2010102039718A CN101855988B (en) 2010-06-17 2010-06-17 Method for culturing fungi and planting tall gastrodia tuber indoors

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CN101855988B true CN101855988B (en) 2012-05-30

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550235A (en) * 2010-12-28 2012-07-11 秦绍新 Cut-log imitated cultivation method of gastrodia elata by utilizing mulberry branches
CN104945121A (en) * 2015-06-16 2015-09-30 王金生 Special fertilizer for gastrodia elata
CN104871804A (en) * 2015-06-19 2015-09-02 习水县龙洋生态食品开发有限公司 Indoor spawn cultivating and breeding method for gastrodia elata
CN105165625A (en) * 2015-10-14 2015-12-23 成都高山乌天麻种植有限公司 Tissue culture type gastrodia elata seed breeding technology
CN109757299B (en) * 2019-02-14 2020-11-24 浙江省农业科学院 Cultivation method for improving quality of ganoderma lucidum spore powder
CN109997603A (en) * 2019-03-19 2019-07-12 南京康之春生物科技有限公司 A kind of cultural method of the artificial Bag Material ganoderma lucidum of optimization

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1107284A (en) * 1994-01-20 1995-08-30 刘喜明 Technique for cultivation of Gastrodia elata
CN2279057Y (en) * 1996-04-17 1998-04-22 张东祥 One-step method and apparatus for planting rhizoma gastrodiae
CN1309885A (en) * 2000-12-09 2001-08-29 崔学发 Industrial culture of Gastrodia elata
CN1473459A (en) * 2003-08-07 2004-02-11 广水市东晨菌业科技有限公司 Synchronously cultivating method for rhizoma gastrodiac sexual propagation
CN101185396A (en) * 2007-12-12 2008-05-28 大方县九龙天麻开发有限公司 Gastrodia tuber imitative wild sexual reproduction breeding technique

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1107284A (en) * 1994-01-20 1995-08-30 刘喜明 Technique for cultivation of Gastrodia elata
CN2279057Y (en) * 1996-04-17 1998-04-22 张东祥 One-step method and apparatus for planting rhizoma gastrodiae
CN1309885A (en) * 2000-12-09 2001-08-29 崔学发 Industrial culture of Gastrodia elata
CN1473459A (en) * 2003-08-07 2004-02-11 广水市东晨菌业科技有限公司 Synchronously cultivating method for rhizoma gastrodiac sexual propagation
CN101185396A (en) * 2007-12-12 2008-05-28 大方县九龙天麻开发有限公司 Gastrodia tuber imitative wild sexual reproduction breeding technique

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