CN108450232A - The method for cultivating agrocybe with mushroom mushroom bran - Google Patents
The method for cultivating agrocybe with mushroom mushroom bran Download PDFInfo
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- CN108450232A CN108450232A CN201810239731.XA CN201810239731A CN108450232A CN 108450232 A CN108450232 A CN 108450232A CN 201810239731 A CN201810239731 A CN 201810239731A CN 108450232 A CN108450232 A CN 108450232A
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- mushroom
- bacterium
- bacterium bag
- agrocybe
- bran
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The present invention relates to the methods for cultivating agrocybe with mushroom mushroom bran, and Lenlinus edodes chaff powder is broken, weighs mushroom bran, cotton seed hulls and sawdust and adds water, so that material moisture is reached 80%, bored heap fermentation；It weighs wheat bran and corn flour is added in fermentation material, moisture mixing in control material, pack, lantern ring sealing；Bacterium bag Co60Gamma-ray irradiation sterilizes, and sterilizing is placed in inoculation room, and tea tree mushroom strains are followed by through surface disinfection, and the bacterium bag after inoculation moves bacterium germination room to, is protected from light bacterium germination, and mycelia covers with bacterium bag, and moving mushroom room to after mycelia curing carries out management of producing mushroom；After buddingging, harvested when mushroom lid is by parachute-opening；The agrocybe of harvesting is dried to get agrocybe product.The present invention is raw materials for production using Lentnus edodes waste bacterium rod, adjusts raw material carbon-nitrogen ratio by adding cotton seed hulls, sawdust, wheat bran, bacterium bag production cost is low, biological transformation ratio is high.Bacterium bag is sterilized instead of wet heat sterilization technology with irradiation technique, sterilization of high efficiency, effect is good.
Description
Technical field
The invention belongs to field of edible fungus culture, and in particular to a method of cultivating agrocybe with mushroom mushroom bran.
Background technology
Agrocybe also known as Agrocybe chaxingu are subordinate to Eumycota, Basidiomycotina, Hymenomycetes, Agaricales, excrement rust umbrella on taxology
Section, Agrocybe.Agrocybe is with rich flavor, excellent taste, full of nutrition, contains 18 kinds of amino acid and a variety of mineral elements,
In 8 kinds of amino acid be essential amino acid.Agrocybe has higher health care and medical value, there is the effect of diuresis, invigorating the spleen,
And have the function of anti-aging, improve immunity, reduce cholesterol, be integrate nutrition, health care, medical function it is rare
Edible and medicinal fungus, is favored by people, civil to be referred to as " refreshing mushroom ".The main cultivation of China edible mushroom is rapidly developed into recent years
Train one of kind.Wild tea trees mushroom is usually happened on the branch and tree root of rotten tea oil tree, the natural deliciousness of wild tea trees mushroom,
Unique flavor, but quantity is extremely rare.
Artificial cultivation agrocybe is usually using cotton seed hulls and sawdust as main raw material(s), with Edible Fungi Industry Development, to original
The demand of material increased dramatically, and bacterium woods contradiction becomes increasingly conspicuous, and the supply of sawdust is gradually limited.In addition, mushroom be China's yield most
Big domestomycetes, the mushroom mushroom bran generated every year is generally discarded or burn-up, the wasting of resources are extremely serious.On the other hand, Lenlinus edodes
Still contain C elements and the N elements such as a large amount of cellulose, hemicellulose, protein in chaff, can grow and provide for agrocybe mycelia
Nutrient.
Bacteria stick after mushroom fruiting is generally directly abandoned or is burned, and recycling rate of waterused is low, and mushroom mycelium is to wooden in culture medium
Quality, cellulose utilization rate are high, still have the carbon sources such as a large amount of cellulose not utilized in the waste bacterium rod after fruiting.
CN106396807A discloses a kind of culture medium and preparation method thereof improving edible fungus living being conversion ratio and edible
The cultural method of bacterium.Culture medium include cotton seed hulls, corncob, beans winnow with a dustpan, wheat bran, Waste compost and calcium carbonate.
CN104770215A discloses a kind of tea tree mushroom cultivation method, by cotton seed hulls, sawdust, tea seed cake, mushroom stem, stone
Ash and precipitated calcium carbonate dispensing；Mushroom stem is the leftover bits and pieces in Lentnus edodes process, is ground into particle after drying；It is fragrant
Mushroom tang particle is added clear water and impregnates, and leachate is uniformly admixed cotton seed hulls, sawdust, tea seed cake together with mushroom stems slag and mixed
In material, spontaneous fermentation is banked up, the culture material fermented is directly made the bed, sowed and bacterium germination is covered when the long full charge layer of mycelia through heat dissipation
Lid loam carries out management of producing mushroom；After the harvesting of third damp mushroom, depending on mycelia growing state, in mushroom bed, sprinkling mushroom stem leaches comprehensively
Liquid.
Invention content
The purpose of the present invention is be directed to above-mentioned present situation, it is desirable to provide a kind of biological transformation ratio is high, at low cost, technically simple
The method and its application method of agrocybe are cultivated with mushroom mushroom bran.
The realization method of the object of the invention is that the method for cultivating agrocybe with mushroom mushroom bran is as follows：
1) Lenlinus edodes chaff powder is broken to 0.5 ± 0.2cm of granular size；
2) it is mushroom bran 20-40%, cotton seed hulls 20-30% and sawdust 10-30% to weigh mass ratio, adds water, keeps material aqueous
Amount reaches 80%, and bored heap fermentation is for 24 hours；
3) it is the fermentation that wheat bran 15-20% and corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In material, moisture is 60-65%, mixing, pack, lantern ring sealing in control material；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, irradiation dose 5-10kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, tea tree mushroom strains is followed by through surface disinfection, after inoculation
Bacterium bag move bacterium germination room to, 15-25 DEG C is protected from light bacterium germination, 40-50 days time；
6) in the indoor bacterium bag of bacterium germination through 40-50 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 200-700lux, humidity>90%, 20-28 DEG C of temperature；
8) it 5-10 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening；
9) agrocybe for harvesting step 8), in 70 DEG C of drying to get agrocybe product.
The present invention is raw materials for production using Lentnus edodes waste bacterium rod, and raw material are adjusted by adding cotton seed hulls, sawdust, wheat bran
Carbon-nitrogen ratio, bacterium bag production cost is low, biological transformation ratio is high.Bacterium bag is sterilized instead of wet heat sterilization technology with irradiation technique,
Sterilization of high efficiency, effect are good.
The applicant obtains the present invention by many experiments, and using the present invention, agrocybe mycelial growth rate is fast, and yield is not
Less than conventional method.Mushroom mycelium growth is more demanding to content of lignin, and agrocybe is low to content of lignin requirement in raw material,
Therefore agrocybe cultivation can be carried out using the waste bacterium rod after mushroom fruiting.It is that major ingredient produces agrocybe tool using mushroom waste bacterium rod
Have the advantages that production cost is low, bacterium germination is fast, is had not been reported on agrocybe.Patent of invention of the present invention has novelty.
Specific implementation mode
The present invention crushes mushroom mushroom bran with wood grinder, weighs mushroom bran, cotton seed hulls and sawdust and adds water, keeps material aqueous
Amount reaches 80%, bored heap fermentation；It weighs wheat bran and corn flour is added in fermentation material or takes mass ratio for wheat bran 10-19%, jade
Rice flour 4% and precipitated calcium carbonate 1% are added in the fermentation material of step 2).
Moisture mixing in control material, pack, lantern ring sealing.Bacterium bag selects 18*35cm polyethylene bacterium bags, per packed
1kg materials.Bacterium bag Co60Gamma-ray irradiation sterilizes, and sterilizing is placed in inoculation room, and tea tree mushroom strains are followed by through surface disinfection,
Bacterium bag after inoculation moves bacterium germination room to, is protected from light bacterium germination, and mycelia covers with bacterium bag, and moving mushroom room to after mycelia curing carries out fruiting pipe
Reason；After buddingging, harvested when mushroom lid is by parachute-opening；The agrocybe of harvesting is dried to get agrocybe product.
The present invention is described in detail with specific embodiment below.
Embodiment 1
1) Lenlinus edodes chaff powder is broken to 0.5 ± 0.2cm of granular size；
2) it is mushroom bran 20%, cotton seed hulls 30% and sawdust 30% to weigh mass ratio, adds water, material moisture is made to reach
80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 15%, corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 65% in control material, mixing, pack, per packed 1kg materials, lantern ring sealing；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, radiation resistance 5kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, tea tree mushroom strains is followed by through surface disinfection, after inoculation
Bacterium bag move bacterium germination room to, 20 DEG C are protected from light bacterium germination, 45 days time；
6) in the indoor bacterium bag of bacterium germination through 45 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 200lux, humidity>90%, 20 DEG C of temperature；
8) it 5 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening；
9) agrocybe for harvesting step 8), in 70 DEG C of drying to get agrocybe product.
Embodiment 2, with example 1, unlike,
2) it is mushroom bran 30%, cotton seed hulls 20% and sawdust 30% to weigh mass ratio, adds water, material moisture is made to reach
80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 15%, corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 62% in control material, mixing, pack, per packed 1kg materials, lantern ring sealing；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, radiation resistance 8kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, kind of an agrocybe is followed by through surface disinfection, after inoculation
Bacterium bag moves bacterium germination room to, and 15 DEG C are protected from light bacterium germination, 50 days time；
6) in the indoor bacterium bag of bacterium germination through 50 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 500lux, humidity>90%, 25 DEG C of temperature,
8) it 10 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening.
Embodiment 3, with example 1, unlike,
2) it is mushroom bran 27%, cotton seed hulls 28% and sawdust 20% to weigh mass ratio, adds water, material moisture is made to reach
80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 20%, corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 65% in control material, mixing, pack, per packed 1kg materials, lantern ring sealing；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, radiation resistance 10kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, tea tree mushroom strains is followed by through surface disinfection, after inoculation
Bacterium bag move bacterium germination room to, 25 DEG C are protected from light bacterium germination, 40 days time；
6) in the indoor bacterium bag of bacterium germination through 40 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 700lux, humidity>90%, 20 DEG C of temperature,
8) it 5 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening.
Embodiment 4, with example 1, unlike,
2) it is mushroom bran 40%, cotton seed hulls 25% and sawdust 10% to weigh mass ratio, adds water, material moisture is made to reach
80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 20%, corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 65% in control material, mixing, pack, per packed 1kg materials, lantern ring sealing；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, radiation resistance 10kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, tea tree mushroom strains is followed by through surface disinfection, after inoculation
Bacterium bag move bacterium germination room to, 20 DEG C are protected from light bacterium germination, 45 days time；
6) in the indoor bacterium bag of bacterium germination through 45 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 700lux, humidity>90%, 28 DEG C of temperature,
8) it 5 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening.
Embodiment 5, with example 1, unlike,
2) it is mushroom bran 30%, cotton seed hulls 25% and sawdust 20% to weigh mass ratio, adds water, material moisture is made to reach
80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 20%, corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 63% in control material, mixing, pack, per packed 1kg materials, lantern ring sealing；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, radiation resistance 10kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, tea tree mushroom strains is followed by through surface disinfection, after inoculation
Bacterium bag move bacterium germination room to, 15 DEG C are protected from light bacterium germination, 50 days time；
6) in the indoor bacterium bag of bacterium germination through 50 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition
It is：Mushroom room scattered light intensity 700lux, humidity>90%, 24 DEG C of temperature,
8) it 5 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening.
Claims (3)
1. with the method for mushroom mushroom bran cultivation agrocybe, it is characterised in that：It is as follows：
1) Lenlinus edodes chaff powder is broken to 0.5 ± 0.2cm of granular size；
2) it is mushroom bran 20-40%, cotton seed hulls 20-30% and sawdust 10-30% to weigh mass ratio, adds water, material moisture is made to reach
To 80%, bored heap fermentation is for 24 hours；
3) it is the fermentation material that wheat bran 15-20% and corn flour 4% and precipitated calcium carbonate 1% are added to step 2) to weigh mass ratio
In, moisture is 60-65%, mixing, pack, lantern ring sealing in control material；
4) by the bacterium bag Co of step 3)60Gamma-ray irradiation sterilizes, irradiation dose 5-10kGy；
5) bacterium bag after step 4) sterilizing is placed in inoculation room, is followed by tea tree mushroom strains through surface disinfection, the bacterium after inoculation
Bag moves bacterium germination room to, and 15-25 DEG C is protected from light bacterium germination, 40-50 days time；
6) in the indoor bacterium bag of bacterium germination through 40-50 days, mycelia covers with bacterium bag, and mycelia cures 10 ± 5 days；
7) it moves mushroom room to through step 6) bacterium bag after curing and carries out management of producing mushroom, budding within 10 ± 5 days, management of producing mushroom condition is：
Mushroom room scattered light intensity 200-700lux, humidity>90%, 20-28 DEG C of temperature；
8) it 5-10 days after step 7) is buddingged, is harvested when mushroom lid is by parachute-opening；
9) agrocybe for harvesting step 8), in 70 DEG C of drying to get agrocybe product.
2. the method according to claim 1 for cultivating agrocybe with mushroom mushroom bran, it is characterised in that：Step 1) wood powder
Broken machine crushes mushroom mushroom bran.
3. the application method of the method according to claim 1 for cultivating agrocybe with mushroom mushroom bran, it is characterised in that：Step
3) bacterium bag selects 18*35cm polyethylene bacterium bags, per packed 1kg materials.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601252A (en) * | 2019-01-25 | 2019-04-12 | 湖南省核农学与航天育种研究所 | A kind of method of green production edible fungi substrate bacterium bag |
| CN111670749A (en) * | 2020-07-09 | 2020-09-18 | 福建天天源生物科技有限公司 | Herbal tremella planting method |
| CN115474506A (en) * | 2022-08-26 | 2022-12-16 | 海南浙江大学研究院 | Oyster mushroom cultivation material taking cotton stalk powder and cotton seed powder as substrates and preparation method thereof |
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| CN103650908A (en) * | 2012-09-25 | 2014-03-26 | 殷俊林 | Method for cultivating agrocybe cylindracea through pleurotus eryngii dregs |
| CN105766372A (en) * | 2016-03-30 | 2016-07-20 | 黑龙江省亚布力林业局 | Microwave production line sterilization method for fungus bags of edible fungi |
| CN107041237A (en) * | 2017-05-05 | 2017-08-15 | 贵州穗农农业科技有限公司 | A kind of cultural method of agrocybe |
| CN107667775A (en) * | 2017-08-08 | 2018-02-09 | 雷燕梅 | A kind of implantation methods of agrocybe |
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2018
- 2018-03-22 CN CN201810239731.XA patent/CN108450232A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101889522A (en) * | 2010-07-28 | 2010-11-24 | 四川农业大学 | Method for planting pleurotus ostreatus by using shitake mushroom dregs |
| CN103650908A (en) * | 2012-09-25 | 2014-03-26 | 殷俊林 | Method for cultivating agrocybe cylindracea through pleurotus eryngii dregs |
| CN105766372A (en) * | 2016-03-30 | 2016-07-20 | 黑龙江省亚布力林业局 | Microwave production line sterilization method for fungus bags of edible fungi |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601252A (en) * | 2019-01-25 | 2019-04-12 | 湖南省核农学与航天育种研究所 | A kind of method of green production edible fungi substrate bacterium bag |
| CN111670749A (en) * | 2020-07-09 | 2020-09-18 | 福建天天源生物科技有限公司 | Herbal tremella planting method |
| CN115474506A (en) * | 2022-08-26 | 2022-12-16 | 海南浙江大学研究院 | Oyster mushroom cultivation material taking cotton stalk powder and cotton seed powder as substrates and preparation method thereof |
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