CN109601252A - A kind of method of green production edible fungi substrate bacterium bag - Google Patents
A kind of method of green production edible fungi substrate bacterium bag Download PDFInfo
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- CN109601252A CN109601252A CN201910074958.8A CN201910074958A CN109601252A CN 109601252 A CN109601252 A CN 109601252A CN 201910074958 A CN201910074958 A CN 201910074958A CN 109601252 A CN109601252 A CN 109601252A
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- bacterium bag
- edible fungi
- ozone
- fungi substrate
- raw material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of methods for producing edible fungi substrate bacterium bag, comprising the following steps: (1) is packed into bacterium bag after mixing edible fungi substrate raw material thoroughly, seals；(2) bacterium bag for obtaining step (1) carries out radiation treatment；(3) Ozone Water is added through in step (2) treated bacterium bag, heat preservation, that is, edible fungi substrate bacterium bag can be produced by obtaining.The production method of edible fungi substrate bacterium bag of the invention uses the non-thermal technologies of irradiation and ozone, changes traditional by the way of high temperature high cooking, hot steam sterilizing, Chemical treatment or infrared Nie Jun；Relative to thermal sterilization, can be saved or so 30 hours using the time of this method production bacterium bag, energy consumption saves 60-80%；Relative to chemical sterilization, chemical residual risk is not present using this method production bacterium bag；It is higher to the nutritional ingredient reservation of bacterium bag relative to microwave sterilization, and energy consumption is lower.Therefore this method is a kind of energy saving, green and environmental protection matrix treatments method.
Description
Technical field
The invention belongs to strain preparation field more particularly to a kind of methods of green production edible fungi substrate bacterium bag.
Background technique
Edible fungi substrate is that the substance of nutrition and good growth ecological environment, rice straw, jade are provided for edible fungi growth
The forestry wastes such as agricultural wastes and sawdust, bark, branch such as rice stalk, corncob, rice husk are all production edible mushroom bases
These wastes are used for Edible Fungi and on the one hand solve the problems, such as that waste is rationally disposed, protected by the good raw material of matter
On the one hand ecological environment provides quantity for Edible Fungi and enriches reproducible raw material, kills two birds with one stone.
Preventing living contaminants is the key that edible mushroom substituting stuff cultivation is succeeded, and edible fungus culturing usually will be to culture thus
Material carries out sterilization treatment, and purpose master is the miscellaneous bacteria for killing or inhibiting in matrix, provides properly to be inoculated into the edible mushroom of matrix
Environment.Heat sterilization is the conventional sterilization mode of edible fungi substrate, generallys use 100 DEG C of high-temperature steams, normal-pressure sterilization 10-
12h, sterilization time needed for this method is long, energy consumption is higher, and long-time high temperature also will cause the loss of the nutrient in matrix.
Sanitizer chemical is also widely used in one of edible fungi substrate production process means, at low cost, but there is residual wind
Danger.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the shortcomings of to mention in background above technology and defect, provide one
Kind green, the method for utilizing non-thermal technologies production edible fungi substrate bacterium bag.
In order to solve the above technical problems, technical solution proposed by the present invention are as follows:
A method of producing edible fungi substrate bacterium bag, comprising the following steps:
(1) it is packed into bacterium bag after mixing edible fungi substrate raw material thoroughly, seals；
(2) bacterium bag for obtaining step (1) carries out radiation treatment；
(3) Ozone Water is added through in step (2) treated bacterium bag, heat preservation, that is, edible fungi substrate bacterium bag can be produced by obtaining.
Above-mentioned method, it is preferred that in the step (2), radiation treatment refers to using gamma-ray irradiation or using electricity
Beamlet irradiation.Irradiation is non-heat treatment process, and making bacterium bag, temperature does not increase during processing, is protected to the nutritional ingredient in bacterium bag
It stays more favorably, and the heat treatments such as energy consumption relative microwave are lower.
Above-mentioned method, it is preferred that the intensity of radiation treatment is 5-50KGy.
Above-mentioned method, it is preferred that in the step (3), ozone Measuring Ozone Concentration in Water is 5-50mg/L.
Above-mentioned method, it is preferred that the humidity after Ozone Water is added in the step (3), in each bacterium bag is maintained at 35-
80%, guarantee the growth of mycelia.
Above-mentioned method, it is preferred that in the step (3), the temperature of heat preservation is 30-80 DEG C, and the time of heat preservation is 1min-
120min.Heat preservation promotes ozone to decompose, and otherwise ozone will affect hypha of edible fungus growth.
Above-mentioned method, it is preferred that in the step (1), the water content of edible fungi substrate raw material is not higher than 10%；Institute
It states edible fungi substrate raw material to be stored in dry, clean environment, storage condition periodically carries out disinfection.
Above-mentioned method, it is preferred that the humidity of the storage condition of the edible fungi substrate raw material is lower than 80%；Every 7-10
It carried out disinfection using ozone, and ozone gas concentration is 10-500mg/m3, retention time 1min-240min.
Above-mentioned method, it is preferred that the edible mushroom includes elegant precious mushroom and flower mushroom.
Compared with the prior art, the advantages of the present invention are as follows:
(1) production method of edible fungi substrate bacterium bag of the invention uses irradiation and two kinds of the ozone non-thermal skills combined
Art changes traditional by the way of high temperature high cooking, hot steam sterilizing, Chemical treatment or infrared Nie Jun；Phase
For thermal sterilization, can be saved or so 30 hours using the time of this method production bacterium bag, energy consumption saves 60-80%；Relative to
Chemical residual risk is not present using this method production bacterium bag in chemical sterilization；Relative to microwave sterilization, to the nutritional ingredient of bacterium bag
Reservation is higher, and energy consumption is lower.Therefore this method is a kind of energy saving, green and environmental protection matrix treatments method.
(2) a large amount of agriculture and forestry organic waste materials can be produced into edible mushroom by the method for production edible fungi substrate bacterium bag of the invention
The matrix of cultivation；And the waste material after edible mushroom culture, organic fertilizer can also be produced, can also dry, be pressed into pellet, shape
At recycling for agriculture and forestry organic waste material, really turn waste into wealth.
Specific embodiment
To facilitate the understanding of the present invention, invention herein is done below in conjunction with preferred embodiment and more comprehensively, is meticulously retouched
It states, but protection scope of the present invention is not limited to following specific embodiments.
Unless otherwise defined, all technical terms used hereinafter are generally understood meaning phase with those skilled in the art
Together.Technical term used herein is intended merely to the purpose of description specific embodiment, and it is of the invention to be not intended to limitation
Protection scope.
Unless otherwise specified, various raw material, reagent, the instrument and equipment etc. used in the present invention can pass through city
Field is commercially available or can be prepared by existing method.
Embodiment 1:
A kind of method of green production elegant precious mushroom matrix bacterium bag, comprising the following steps:
(1) raw material preparation:
The raw material (straw, sawdust, corncob, wheat bran and lime) that water content is lower than 10% is got out, being stored in humidity is
In 60% environment, the every 10 days progress ozonizations in place, ozone phase concentration about 50mg/m are stored3, the ozone retention time
30min。
(2) raw material is packed:
It will be packed into bacterium bag after the raw material that step (1) obtains is mixed thoroughly, sealed.
(3) radiation treatment of bacterium bag:
Radiation treatment will be carried out with gamma-rays by the bacterium bag that step (2) obtain, the intensity of radiation treatment is 8KGy.
(4) Ozone Water is prepared:
It is passed through ozone into tap water, so that ozone and tap water is carried out mixture, the Ozone Water of ozone concentration 20mg/L is made.
(5) bacterium bag humidifies:
In gnotobasis, the Ozone Water prepared through step (4) is added in the bacterium bag that step (3) obtains immediately, is added
Make bacterium bag humidity about 65% after wet.
(6) bacterium bag is kept the temperature:
Bacterium bag will be obtained through step (5) and sets to keep 1h in 50 DEG C of environment.
(7) bacterium bag is inoculated with:
Elegant precious mushroom strain is accessed in gnotobasis.
Comparative example 1:
This comparative example uses produced in conventional processes elegant precious mushroom matrix bacterium bag, comprising the following steps:
(1) raw material preparation:
The raw material (straw, sawdust, corncob, wheat bran and lime) that water content is lower than 10% is got out, dry ring is stored in
In border.
(2) spice and packaging:
It will be stirred after the raw material that step (1) obtains adds water, mix moisture content about 65% in rear matrix thoroughly, be packed into bacterium
Bag, sealing.
(3) bacterium bag is sterilized:
Bacterium bag will be obtained by step (2) is packed into sterilization container, heating is in bacterium bag and is not less than at a temperature of 100 DEG C,
Kept for about 10 hours；
(4) cooling inoculation:
Elegant precious mushroom strain is accessed in gnotobasis after bacterium bag completely cooling (about 24 hours).
The scheme of embodiment 1 produces about 6 hours qualified bacterium bag time with expecting from matrix, at least relative to conventional method
It can save 30 hours, the technical solution energy consumption cost of additional embodiment 1 is lower than 0.1 yuan/kg, and traditional heat sterilization energy consumption cost is about
0.25-0.35 member/kg saves 60% or more using the solution of the present invention energy consumption.
The matrix bacterium bag that embodiment 1 produces even more is better than traditional use to the no adverse effect of the growth of elegant precious mushroom
Thermal sterilization method (is shown in Table shown in 1-3).
The influence of bacterium bag prepared by 1 embodiment 1 of table and comparative example 1 to mycelia
The influence of bacterium bag prepared by 2 embodiment 1 of table and comparative example 1 to fruiting time
The influence of bacterium bag prepared by 3 embodiment 1 of table and comparative example 1 to biological efficiency
Embodiment 2:
A kind of method of green production flower mushroom matrix bacterium bag of the invention, comprising the following steps:
(1) raw material preparation:
The raw material (sawdust, corncob, wheat bran, dipotassium hydrogen phosphate and gypsum) that water content is lower than 8% is got out, is stored in wet
The environment that degree is 50% stores the every 7 days progress ozonizations in place, ozone concentration about 30mg/m3, ozone retention time 1h.
(2) raw material is packed:
It will be packed into bacterium bag after the raw material that step (1) obtains is mixed thoroughly, sealed.
(3) radiation treatment of bacterium bag:
Radiation treatment will be carried out with electron beam by the bacterium bag that step (2) obtain, the intensity of radiation treatment is 15KGy.
(4) Ozone Water is prepared:
It is passed through ozone into tap water, ozone and tap water is made to carry out mixture, the ozone that ozone concentration is 30mg/L is made
Water.
(5) bacterium bag humidifies:
In gnotobasis, the Ozone Water prepared through step (4) is added to immediately in the bacterium bag obtained through step (3),
Make bacterium bag humidity about 65% after humidification.
(6) bacterium bag is kept the temperature:
Bacterium bag will be obtained through step (5) and is placed in 60 DEG C of environment to keep 1h.
(7) bacterium bag is inoculated with:
Flower mushroom strain is accessed in gnotobasis.
Comparative example 2:
This comparative example uses produced in conventional processes elegant precious mushroom matrix bacterium bag, comprising the following steps:
(1) raw material preparation:
The raw material (sawdust, corncob, wheat bran, dipotassium hydrogen phosphate and gypsum) that water content is lower than 8% is got out, is stored in dry
In dry environment.
(2) spice and packaging:
It will be stirred after the raw material that step (1) obtains adds water, mix moisture content about 65% in rear matrix thoroughly, be packed into bacterium
Bag, sealing.
(3) bacterium bag is sterilized:
Bacterium bag will be obtained by step (2) is packed into sterilization container, heating is in bacterium bag and is not less than at a temperature of 100 DEG C,
Kept for about 10 hours.
(4) cooling inoculation:
Flower mushroom strain is accessed in gnotobasis after bacterium bag completely cooling (about 24 hours).
Embodiment 2 produces about 8 hours qualified bacterium bag time with expecting from matrix, can at least save relative to conventional method
It 30 hours, in addition, 2 energy consumption cost of embodiment is lower than 0.1 yuan/kg, and tradition heat sterilization energy consumption cost about 0.3-0.4 member/kg, adopts
60% or more is saved with the energy consumption of embodiment 2.
The matrix bacterium bag that embodiment 2 produces even more is better than traditional use to the no adverse effect of the growth of elegant precious mushroom
Thermal sterilization method (is shown in Table shown in 4-6).
The influence of bacterium bag prepared by 4 embodiment 2 of table and comparative example 2 to mycelia
The influence of bacterium bag prepared by 5 embodiment 2 of table and comparative example 2 to fruiting time
The influence of bacterium bag prepared by 6 embodiment 2 of table and comparative example 2 to biological efficiency
Claims (9)
1. a kind of method of green production edible fungi substrate bacterium bag, which comprises the following steps:
(1) it is packed into bacterium bag after mixing edible fungi substrate raw material thoroughly, seals；
(2) bacterium bag for obtaining step (1) carries out radiation treatment；
(3) Ozone Water is added through in step (2) treated bacterium bag, heat preservation obtains production edible fungi substrate bacterium bag.
2. the method as described in claim 1, which is characterized in that in the step (2), radiation treatment refers to using gamma-rays spoke
According to or using electron beam irradiation.
3. method according to claim 2, which is characterized in that the intensity of radiation treatment is 5-50KGy.
4. the method as described in claim 1, which is characterized in that in the step (3), ozone Measuring Ozone Concentration in Water is 5-
50mg/L。
5. the method as described in claim 1, which is characterized in that in the step (3), after Ozone Water is added in each bacterium bag
Humidity is maintained at 35-80%.
6. the method as described in claim 1, which is characterized in that in the step (3), the temperature of heat preservation is 30-80 DEG C, heat preservation
Time be 1min-120min.
7. as the method according to claim 1 to 6, which is characterized in that in the step (1), edible fungi substrate raw material
Water content be not higher than 10%；The edible fungi substrate raw material are stored in dry, clean environment, and storage condition is regular
It carries out disinfection.
8. the method for claim 7, which is characterized in that the humidity of the storage condition of the edible fungi substrate raw material is low
In 80%；It carries out within every 7-10 days carrying out disinfection using ozone, ozone gas concentration is 10-500mg/m3, retention time 1min-
240min。
9. as the method according to claim 1 to 6, which is characterized in that the edible mushroom includes elegant precious mushroom and flower mushroom.
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| CN201910074958.8A CN109601252A (en) | 2019-01-25 | 2019-01-25 | A kind of method of green production edible fungi substrate bacterium bag |
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| CN201910074958.8A CN109601252A (en) | 2019-01-25 | 2019-01-25 | A kind of method of green production edible fungi substrate bacterium bag |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046831A (en) * | 1990-06-29 | 1990-11-14 | 张百万 | Edible mushroom ozone bacterium-controlling cultivating method |
| CN104272986A (en) * | 2013-07-02 | 2015-01-14 | 宋燕文 | Grown plant seedling detoxification and disinfection method |
| CN105993726A (en) * | 2016-05-05 | 2016-10-12 | 上海师范大学 | Vegetable disease control method |
| CN107821569A (en) * | 2017-11-03 | 2018-03-23 | 百洋产业投资集团股份有限公司 | A kind of preservation method of Tilapia mossambica |
| CN108450232A (en) * | 2018-03-22 | 2018-08-28 | 湖北省农业科学院农产品加工与核农技术研究所 | The method for cultivating agrocybe with mushroom mushroom bran |
| CN108450242A (en) * | 2018-03-22 | 2018-08-28 | 淮北智淮科技有限公司 | A kind of Agrocybe cylindracea culture medium and its preparation process |
| CN108551975A (en) * | 2018-04-17 | 2018-09-21 | 北京亚盛增光物理农业科技开发有限公司 | A kind of energy-saving and environment-friendly bacteria stick manufacturing method |
-
2019
- 2019-01-25 CN CN201910074958.8A patent/CN109601252A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046831A (en) * | 1990-06-29 | 1990-11-14 | 张百万 | Edible mushroom ozone bacterium-controlling cultivating method |
| CN104272986A (en) * | 2013-07-02 | 2015-01-14 | 宋燕文 | Grown plant seedling detoxification and disinfection method |
| CN105993726A (en) * | 2016-05-05 | 2016-10-12 | 上海师范大学 | Vegetable disease control method |
| CN107821569A (en) * | 2017-11-03 | 2018-03-23 | 百洋产业投资集团股份有限公司 | A kind of preservation method of Tilapia mossambica |
| CN108450232A (en) * | 2018-03-22 | 2018-08-28 | 湖北省农业科学院农产品加工与核农技术研究所 | The method for cultivating agrocybe with mushroom mushroom bran |
| CN108450242A (en) * | 2018-03-22 | 2018-08-28 | 淮北智淮科技有限公司 | A kind of Agrocybe cylindracea culture medium and its preparation process |
| CN108551975A (en) * | 2018-04-17 | 2018-09-21 | 北京亚盛增光物理农业科技开发有限公司 | A kind of energy-saving and environment-friendly bacteria stick manufacturing method |
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