CN1210066C - 用来自分化细胞的供体核克隆猪的方法 - Google Patents
用来自分化细胞的供体核克隆猪的方法 Download PDFInfo
- Publication number
- CN1210066C CN1210066C CNB988079569A CN98807956A CN1210066C CN 1210066 C CN1210066 C CN 1210066C CN B988079569 A CNB988079569 A CN B988079569A CN 98807956 A CN98807956 A CN 98807956A CN 1210066 C CN1210066 C CN 1210066C
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- cells
- disease
- nuclear transfer
- porcine
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8778—Swine embryos
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0273—Cloned vertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2510/00—Genetically modified cells
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- C12N2517/00—Cells related to new breeds of animals
- C12N2517/02—Cells from transgenic animals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2517/00—Cells related to new breeds of animals
- C12N2517/04—Cells produced using nuclear transfer
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Genetics & Genomics (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Neurology (AREA)
- Animal Husbandry (AREA)
- Neurosurgery (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cardiology (AREA)
- Diabetes (AREA)
- Heart & Thoracic Surgery (AREA)
- Dermatology (AREA)
- Virology (AREA)
- Physical Education & Sports Medicine (AREA)
- Cell Biology (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/888,057 US6235969B1 (en) | 1997-01-10 | 1997-07-03 | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US08/888,057 | 1997-07-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1265600A CN1265600A (zh) | 2000-09-06 |
| CN1210066C true CN1210066C (zh) | 2005-07-13 |
Family
ID=25392430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB988079569A Expired - Lifetime CN1210066C (zh) | 1997-07-03 | 1998-07-01 | 用来自分化细胞的供体核克隆猪的方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US6235969B1 (enExample) |
| EP (1) | EP1017423A4 (enExample) |
| JP (1) | JP2001509362A (enExample) |
| CN (1) | CN1210066C (enExample) |
| AU (1) | AU742840B2 (enExample) |
| BR (1) | BR9811659A (enExample) |
| CA (1) | CA2295294A1 (enExample) |
| IL (1) | IL133785A0 (enExample) |
| NZ (1) | NZ502124A (enExample) |
| WO (1) | WO1999001164A1 (enExample) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9517779D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US20090092588A1 (en) * | 1997-01-10 | 2009-04-09 | University Of Massachusetts As Represented By Its Amherst Campus | Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease |
| US6235969B1 (en) * | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US7291764B1 (en) * | 1997-01-10 | 2007-11-06 | University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst | Cloning pigs using non-quiescent differentiated donor cells or nuclei |
| US6331659B1 (en) | 1998-01-21 | 2001-12-18 | University Of Hawaii | Cumulus cells as nuclear donors |
| JP2002511234A (ja) * | 1998-03-16 | 2002-04-16 | リレグ ピーティーワイ リミテッド | ブタの核移植 |
| JPH11289916A (ja) * | 1998-04-09 | 1999-10-26 | Kazuo Shiga | 牛の筋肉組織の培養細胞を用いて受胎可能な体細胞核移植クローン胚の 作出及びクローン胚を凍結保存する方法 |
| AUPP294898A0 (en) * | 1998-04-15 | 1998-05-07 | Monash University | Method of nuclear transfer |
| GB2365445A (en) * | 1998-10-12 | 2002-02-20 | Geron Bio Med Ltd | Porcine occcytes with improved developmental competence |
| US6781030B1 (en) | 1998-11-02 | 2004-08-24 | Trustee Of Tufts College, Ballou Hall | Methods for cloning mammals using telophase oocytes |
| AU780754B2 (en) | 1999-03-04 | 2005-04-14 | Revivicor Inc | Genetic modification of somatic cells and uses thereof |
| US6911429B2 (en) | 1999-04-01 | 2005-06-28 | Transition Therapeutics Inc. | Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans |
| US6864235B1 (en) | 1999-04-01 | 2005-03-08 | Eva A. Turley | Compositions and methods for treating cellular response to injury and other proliferating cell disorders regulated by hyaladherin and hyaluronans |
| WO2001013826A1 (de) * | 1999-08-20 | 2001-03-01 | Metz Stavenhagen Peter | Wirbelsäulensegment |
| US20040180430A1 (en) * | 1999-09-07 | 2004-09-16 | West Michael D. | Methods of restoring telomere length and extending cell lifespan using nuclear transfer |
| US20050255596A1 (en) * | 1999-09-07 | 2005-11-17 | West Michael D | Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer |
| EP1241931A4 (en) * | 1999-09-13 | 2004-12-29 | Univ Massachusetts | CLONING PIGS USING DONORATION CELLS OR NUCLEAR CELLS OF DIFFERENTIATED CELLS AND PRODUCING PLURIPOTENT PIGS |
| CA2426669A1 (en) * | 1999-10-22 | 2001-05-03 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | .alpha.1-3 galactosyltransferase gene and promoter |
| KR100417566B1 (ko) * | 1999-11-19 | 2004-02-05 | 한국생명공학연구원 | 체세포 핵치환 복제수정란의 대량생산방법 |
| US20060174358A1 (en) * | 2001-01-04 | 2006-08-03 | Xiangzhong Yang | Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells |
| US7071372B2 (en) * | 2000-01-04 | 2006-07-04 | University Of Connecticut | Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells |
| CA2403344C (en) * | 2000-03-15 | 2013-05-21 | The University Of Georgia Research Foundation, Inc. | Effective nuclear reprogramming in mammals |
| US20040033600A1 (en) | 2001-03-21 | 2004-02-19 | Palli Subba Reddy | Ecdysone receptor-based inducible gene expression system |
| GB2360522A (en) | 2000-03-24 | 2001-09-26 | Geron Corp | A strategy for maintaining pregnancy |
| AU2001247732A1 (en) * | 2000-03-24 | 2001-10-08 | Geron Corporation | A strategy for maintaining pregnancy |
| US20030172394A1 (en) * | 2001-08-01 | 2003-09-11 | Holzer George L | Selection and cloning methods |
| CA2634294A1 (en) | 2000-08-03 | 2002-02-14 | Therapeutic Human Polyclonals, Inc. | Production of humanized antibodies in transgenic animals |
| US8105825B2 (en) * | 2000-10-03 | 2012-01-31 | Intrexon Corporation | Multiple inducible gene regulation system |
| US7491534B2 (en) * | 2000-12-22 | 2009-02-17 | Kirin Holdings Kabushiki Kaisha | Methods for altering cell fate to generate T-cells specific for an antigen of interest |
| AU2002232858B2 (en) * | 2000-12-22 | 2007-01-11 | Sab, Llc | Methods for cloning mammals using reprogrammed donor chromatin or donor cells |
| US20020142397A1 (en) * | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
| WO2002074078A2 (en) | 2001-01-30 | 2002-09-26 | Rebholtz, Amy, K. | Method for embryo after impregnation of the recipient female |
| JP4955905B2 (ja) * | 2001-02-20 | 2012-06-20 | イントレキソン コーポレーション | キメラレチノイドx受容体および新規エクジソン受容体−ベースの誘導性遺伝子発現システムにおけるそれらの使用 |
| WO2003005810A2 (en) * | 2001-07-09 | 2003-01-23 | Alexion Pharmaceuticals Inc. | Cloned non-human mammals from contact inhibited donor cells |
| EP1465986A4 (en) * | 2001-12-29 | 2005-01-26 | Woo Suk Hwang | GFP-TRANSFECTED CLONING PIG, GT-KNOCKOUT CLONING PIG, AND METHOD FOR THE MANUFACTURE THEREOF |
| WO2003060083A2 (en) * | 2002-01-15 | 2003-07-24 | Advanced Cell Technology, Inc. | Cloning b and t lymphocytes |
| US20050170506A1 (en) * | 2002-01-16 | 2005-08-04 | Primegen Biotech Llc | Therapeutic reprogramming, hybrid stem cells and maturation |
| US20050090004A1 (en) * | 2003-01-16 | 2005-04-28 | Sayre Chauncey B. | Stem cell maturation for all tissue lines |
| US20030217378A1 (en) * | 2002-04-05 | 2003-11-20 | The University Of Georgia Research Foundation, Inc | Cloning using rapidly matured oocytes |
| JP2005523031A (ja) * | 2002-04-24 | 2005-08-04 | ユニバーシテイ・オブ・ジヨージア・リサーチ・フアウンデイシヨン | 冷却死骸からのクローン化された仔の生産 |
| US7612250B2 (en) * | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
| EP1534819B1 (en) | 2002-08-21 | 2009-12-09 | Revivicor, Inc. | Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US20050034175A1 (en) * | 2002-09-09 | 2005-02-10 | Latif Zuhair A. | Immune cloning and uses thereof |
| EP1581636A4 (en) * | 2002-10-16 | 2006-09-20 | Advanced Cell Tech Inc | METHOD OF USE OF STEM CELLS TREATED BY DERGENVALENTECHNIK (GENE TRAP) FOR MARKING STRAIN CELL DIFFERENTIATION PATHS, AND THE PREPARATION AND ISOLATION OF DIFFERENTIATED CELLS |
| US20040077077A1 (en) * | 2002-10-18 | 2004-04-22 | Xiangzhong Yang | Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same |
| CN1683524A (zh) * | 2003-04-08 | 2005-10-19 | 东南大学 | 用体细胞核移植技术制备移核胚胎干细胞的方法 |
| AU2004290006A1 (en) * | 2003-11-05 | 2005-05-26 | University Of Pittsburgh | Porcine isogloboside 3 synthase protein, cDNA, genomic organization, and regulatory region |
| CA2857051A1 (en) | 2004-03-17 | 2005-09-29 | David Ayares | Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US20050277147A1 (en) * | 2004-06-11 | 2005-12-15 | Ameet Patki | Identifying chromosomal abnormalities in cells obtained from follicular fluid |
| US20060080746A1 (en) * | 2004-09-28 | 2006-04-13 | Viagen, Inc. | Methods of embryo transfer |
| KR100733012B1 (ko) * | 2005-07-26 | 2007-06-28 | 재단법인서울대학교산학협력재단 | 복제된 개과 동물 및 이의 생산 방법 |
| CA2617930A1 (en) | 2005-08-09 | 2007-03-29 | Revivicor, Inc. | Transgenic ungulates expressing ctla4-ig and uses thereof |
| WO2008085891A1 (en) * | 2007-01-05 | 2008-07-17 | Worcester Polytechnic Institute | Oocyte spindle-associated factors improve somatic cell cloning |
| KR100829426B1 (ko) | 2007-01-17 | 2008-05-15 | 재단법인서울대학교산학협력재단 | 복제개의 생산 방법 |
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| JP6267785B2 (ja) | 2013-04-30 | 2018-01-24 | コングク・ユニバーシティ・インダストリアル・コーペレイション・コーポレイション | Cmp−アセチルノイラミン酸ヒドロキシラーゼタゲッティングベクター、そのベクターが導入された異種間移植用形質転換動物及びその製造方法 |
| CN103343142B (zh) * | 2013-07-15 | 2017-07-25 | 云南农业大学 | 一种利用连续克隆技术生产孤雌生殖克隆猪的方法 |
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| CN112759633B (zh) * | 2021-01-07 | 2022-08-12 | 江西农业大学 | 一种可用于建立猪癌症模型的蛋白及其核酸 |
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| IL133786A0 (en) * | 1997-07-03 | 2001-04-30 | Univ Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
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- 1998-07-01 NZ NZ502124A patent/NZ502124A/xx unknown
- 1998-07-01 IL IL13378598A patent/IL133785A0/xx unknown
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- 1998-07-01 BR BR9811659-2A patent/BR9811659A/pt not_active Application Discontinuation
- 1998-07-01 CN CNB988079569A patent/CN1210066C/zh not_active Expired - Lifetime
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- 1998-07-01 AU AU83745/98A patent/AU742840B2/en not_active Ceased
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2004
- 2004-04-02 US US10/817,573 patent/US20040194159A1/en not_active Abandoned
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2006
- 2006-11-22 US US11/603,036 patent/US20070067861A1/en not_active Abandoned
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| WO1999001164A1 (en) | 1999-01-14 |
| NZ502124A (en) | 2003-01-31 |
| BR9811659A (pt) | 2000-09-05 |
| IL133785A0 (en) | 2001-04-30 |
| US20020035737A1 (en) | 2002-03-21 |
| US6235969B1 (en) | 2001-05-22 |
| CN1265600A (zh) | 2000-09-06 |
| EP1017423A4 (en) | 2002-01-30 |
| EP1017423A1 (en) | 2000-07-12 |
| CA2295294A1 (en) | 1999-01-14 |
| AU742840B2 (en) | 2002-01-10 |
| AU8374598A (en) | 1999-01-25 |
| US20070067861A1 (en) | 2007-03-22 |
| JP2001509362A (ja) | 2001-07-24 |
| US20040194159A1 (en) | 2004-09-30 |
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