CN120457210A - 萤光素酶突变体 - Google Patents

萤光素酶突变体

Info

Publication number
CN120457210A
CN120457210A CN202480006843.4A CN202480006843A CN120457210A CN 120457210 A CN120457210 A CN 120457210A CN 202480006843 A CN202480006843 A CN 202480006843A CN 120457210 A CN120457210 A CN 120457210A
Authority
CN
China
Prior art keywords
amino acid
seq
acid residue
substituted
position corresponding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202480006843.4A
Other languages
English (en)
Chinese (zh)
Inventor
稻本千晶
一柳敦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Publication of CN120457210A publication Critical patent/CN120457210A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202480006843.4A 2023-01-27 2024-01-26 萤光素酶突变体 Pending CN120457210A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2023-010710 2023-01-27
JP2023010710 2023-01-27
PCT/JP2024/002442 WO2024158050A1 (ja) 2023-01-27 2024-01-26 ルシフェラーゼ変異体

Publications (1)

Publication Number Publication Date
CN120457210A true CN120457210A (zh) 2025-08-08

Family

ID=91970718

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202480006843.4A Pending CN120457210A (zh) 2023-01-27 2024-01-26 萤光素酶突变体

Country Status (4)

Country Link
EP (1) EP4656723A1 (https=)
JP (1) JPWO2024158050A1 (https=)
CN (1) CN120457210A (https=)
WO (1) WO2024158050A1 (https=)

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2666561B2 (ja) 1990-03-27 1997-10-22 キッコーマン株式会社 変異型ホタルルシフェラーゼ、変異型ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ホタルルシフェラーゼの製造法
JP3048466B2 (ja) 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
DE69633171T2 (de) 1995-01-20 2005-08-18 The Secretary Of State For Defence, Salisbury Mutierte luziferasen
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
DE69841292D1 (de) 1997-07-08 2009-12-31 Kikkoman Corp Mutiertes Biolumineszenz-Protein und Verfahren zu dessen Herstellung
CA2301828A1 (en) 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
JP3749628B2 (ja) 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3681911B2 (ja) 1999-01-05 2005-08-10 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
JP3694181B2 (ja) 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
NZ518413A (en) 1999-10-26 2004-11-26 Secr Defence Novel enzyme
GB0516455D0 (en) 2005-08-10 2005-09-14 Lumora Ltd pH tolerant luciferase
JP4899046B2 (ja) 2005-09-30 2012-03-21 国立大学法人 東京大学 新規ルシフェリン誘導体
AU2008239654A1 (en) 2007-04-13 2008-10-23 Promega Corporation Luminescent live and dead cell assay
JP2011120559A (ja) 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
JP5587644B2 (ja) 2010-03-15 2014-09-10 キッコーマン株式会社 ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
WO2016070788A1 (zh) 2014-11-04 2016-05-12 南车戚墅堰机车车辆工艺研究所有限公司 密接式车钩用风管连接器及密接式车钩用风管连接器组件
JP7175881B2 (ja) 2016-10-13 2022-11-21 プロメガ コーポレイション ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ
KR20190084096A (ko) 2016-11-10 2019-07-15 베링거 인겔하임 인터내셔날 게엠베하 약제학적 조성물, 치료 방법 및 이의 용도
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
WO2019193391A2 (en) 2018-04-04 2019-10-10 Pratik Sharma Managing device farms in cloud
WO2019236731A1 (en) 2018-06-05 2019-12-12 Promega Corporation Luciferase enzymes for use with thermostable luciferins in bioluminescent assays
WO2020009215A1 (ja) 2018-07-06 2020-01-09 キッコーマン株式会社 ルシフェラーゼ変異体

Also Published As

Publication number Publication date
WO2024158050A1 (ja) 2024-08-02
JPWO2024158050A1 (https=) 2024-08-02
EP4656723A1 (en) 2025-12-03

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