CN1197876C - 含有分子内伴侣样序列的嵌合蛋白及其在胰岛素生产中的应用 - Google Patents

含有分子内伴侣样序列的嵌合蛋白及其在胰岛素生产中的应用 Download PDF

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Publication number
CN1197876C
CN1197876C CNB988139413A CN98813941A CN1197876C CN 1197876 C CN1197876 C CN 1197876C CN B988139413 A CNB988139413 A CN B988139413A CN 98813941 A CN98813941 A CN 98813941A CN 1197876 C CN1197876 C CN 1197876C
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peptide section
peptide
amino acid
residue
chimeric protein
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Expired - Lifetime
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CNB988139413A
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Chinese (zh)
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CN1291199A (zh
Inventor
甘忠如
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Beijing Hegenze Source Technology Co Ltd
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Tonghua Gantech Biotechnology Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNB988139413A 1998-03-31 1998-03-31 含有分子内伴侣样序列的嵌合蛋白及其在胰岛素生产中的应用 Expired - Lifetime CN1197876C (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN1998/000052 WO1999050302A1 (en) 1998-03-31 1998-03-31 Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production

Publications (2)

Publication Number Publication Date
CN1291199A CN1291199A (zh) 2001-04-11
CN1197876C true CN1197876C (zh) 2005-04-20

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ID=4575041

Family Applications (1)

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CNB988139413A Expired - Lifetime CN1197876C (zh) 1998-03-31 1998-03-31 含有分子内伴侣样序列的嵌合蛋白及其在胰岛素生产中的应用

Country Status (15)

Country Link
US (1) US6924120B1 (enExample)
EP (1) EP1066328B1 (enExample)
JP (1) JP4472870B2 (enExample)
KR (1) KR100554490B1 (enExample)
CN (1) CN1197876C (enExample)
AT (1) ATE408631T1 (enExample)
AU (1) AU765574B2 (enExample)
BR (1) BR9815788B1 (enExample)
CA (1) CA2324513C (enExample)
DE (1) DE69840035D1 (enExample)
DK (1) DK1066328T3 (enExample)
ES (1) ES2317665T3 (enExample)
IL (1) IL138658A0 (enExample)
MX (1) MXPA00009564A (enExample)
WO (1) WO1999050302A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103439512A (zh) * 2013-07-30 2013-12-11 通化东宝药业股份有限公司 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2372199A1 (en) * 1999-05-14 2000-11-23 Medical Research Council Oligomeric chaperone proteins
JP2003502024A (ja) * 1999-05-14 2003-01-21 メディカル リサーチ カウンシル タンパク質骨格および単量体ポリペプチドを多量体化するためのその使用
GB9913437D0 (en) * 1999-06-09 1999-08-11 Medical Res Council Fusion proteins
US20020164712A1 (en) * 2000-12-11 2002-11-07 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence
EP2336164A1 (en) 2005-12-22 2011-06-22 Genentech, Inc. Recombinant production of heparin binding proteins
WO2008008975A2 (en) 2006-07-14 2008-01-17 Genentech, Inc. Refolding of recombinant proteins
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
RU2447149C1 (ru) * 2011-03-24 2012-04-10 Винсорт Менеджемент Инк РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pMSIN4, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД - ПРЕДШЕСТВЕННИК ИНСУЛИНА ЧЕЛОВЕКА, ШТАММ BL21(DE3)/pMSIN4-ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА
CN102628072B (zh) * 2012-03-20 2013-12-04 甘李药业股份有限公司 一种胰岛素的制备方法
CN112142848A (zh) * 2019-06-26 2020-12-29 中国科学院大连化学物理研究所 一种重组人胰岛素及其纯化制备方法
CN114805610B (zh) * 2022-06-23 2022-10-04 北京惠之衡生物科技有限公司 一种高表达甘精胰岛素前体的重组基因工程菌及其构建方法
CN114805544B (zh) * 2022-06-23 2022-09-09 北京惠之衡生物科技有限公司 一种赖脯胰岛素前体、其重组基因工程菌及其构建方法

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US4342832A (en) 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
NZ199391A (en) 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US5254463A (en) 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4665160A (en) * 1982-03-22 1987-05-12 Genentech, Inc. Novel human growth hormone like protein HGH-V encoded in the human genome
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US5191063A (en) 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US5358857A (en) 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
US5422110A (en) 1991-10-16 1995-06-06 University Of Saskatchewan Enhanced immunogenicity using leukotoxin chimeras
SG46612A1 (en) 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals
US6001604A (en) 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DK0871474T4 (da) * 1994-12-29 2012-01-09 Ferring Int Ct Sa Fremstilling af human insulin
CA2212006A1 (en) * 1995-02-03 1996-08-08 G.D. Searle & Co. Novel c-mpl ligands
US5559128A (en) 1995-04-18 1996-09-24 Merck & Co., Inc. 3-substituted piperidines promote release of growth hormone
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
AUPO394896A0 (en) 1996-11-29 1997-01-02 May, Peter Andrew John Hose mounting bracket
US20020164712A1 (en) * 2000-12-11 2002-11-07 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence
WO2002079251A2 (en) * 2001-04-02 2002-10-10 Novo Nordisk A/S Method for making human insulin precursors

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103439512A (zh) * 2013-07-30 2013-12-11 通化东宝药业股份有限公司 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法
CN103439512B (zh) * 2013-07-30 2014-05-07 通化东宝药业股份有限公司 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法

Also Published As

Publication number Publication date
BR9815788B1 (pt) 2013-06-04
ATE408631T1 (de) 2008-10-15
AU6716498A (en) 1999-10-18
EP1066328B1 (en) 2008-09-17
CA2324513C (en) 2013-05-28
CA2324513A1 (en) 1999-10-07
WO1999050302A1 (en) 1999-10-07
IL138658A0 (en) 2001-10-31
EP1066328A4 (en) 2002-06-05
EP1066328A1 (en) 2001-01-10
US6924120B1 (en) 2005-08-02
DK1066328T3 (da) 2009-02-09
JP4472870B2 (ja) 2010-06-02
JP2004505601A (ja) 2004-02-26
KR20010042383A (ko) 2001-05-25
AU765574B2 (en) 2003-09-25
DE69840035D1 (de) 2008-10-30
MXPA00009564A (es) 2004-03-10
KR100554490B1 (ko) 2006-03-03
ES2317665T3 (es) 2009-04-16
BR9815788A (pt) 2000-11-28
CN1291199A (zh) 2001-04-11

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C06 Publication
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SE01 Entry into force of request for substantive examination
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Owner name: BEIJING YUANHE GENZE TECHNOLOGY CO., LTD.

Free format text: FORMER OWNER: TONGHUA ANTAIKE BIOENGINEERING CO., LTD.

Effective date: 20121217

C41 Transfer of patent application or patent right or utility model
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Effective date of registration: 20121217

Address after: 101102, Beijing, Zhongguancun Tongzhou District science and Technology Park, Tongzhou Park Jinqiao technology industry base, King Sheng North First Street, No. 19-43

Patentee after: Beijing Hegenze Source Technology Co. Ltd.

Address before: Dongbao village, Tonghua, Jilin

Patentee before: Tonghua Antech Biotechnology Ltd.

PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20121204

Granted publication date: 20050420

Pledgee: National Development Bank

Pledgor: Tonghua Antech Biotechnology Ltd.

Registration number: 200622304

PLDC Enforcement, change and cancellation of contracts on pledge of patent right or utility model
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20010411

Assignee: Gan & Lee Pharmaceutical Co., Ltd.

Assignor: Beijing Hegenze Source Technology Co. Ltd.

Contract record no.: 2013990000115

Denomination of invention: Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production

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Record date: 20130326

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