CA2324513C - Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production - Google Patents

Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production Download PDF

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Publication number
CA2324513C
CA2324513C CA2324513A CA2324513A CA2324513C CA 2324513 C CA2324513 C CA 2324513C CA 2324513 A CA2324513 A CA 2324513A CA 2324513 A CA2324513 A CA 2324513A CA 2324513 C CA2324513 C CA 2324513C
Authority
CA
Canada
Prior art keywords
chimeric protein
insulin
amino acid
precursor
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2324513A
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English (en)
French (fr)
Other versions
CA2324513A1 (en
Inventor
Zhongru Gan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BEIJING YUANHEGENZE TECHNOLOGY Co
Original Assignee
BEIJING YUANHEGENZE TECHNOLOGY Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=4575041&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2324513(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by BEIJING YUANHEGENZE TECHNOLOGY Co filed Critical BEIJING YUANHEGENZE TECHNOLOGY Co
Publication of CA2324513A1 publication Critical patent/CA2324513A1/en
Application granted granted Critical
Publication of CA2324513C publication Critical patent/CA2324513C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2324513A 1998-03-31 1998-03-31 Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production Expired - Fee Related CA2324513C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN1998/000052 WO1999050302A1 (en) 1998-03-31 1998-03-31 Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production

Publications (2)

Publication Number Publication Date
CA2324513A1 CA2324513A1 (en) 1999-10-07
CA2324513C true CA2324513C (en) 2013-05-28

Family

ID=4575041

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2324513A Expired - Fee Related CA2324513C (en) 1998-03-31 1998-03-31 Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production

Country Status (15)

Country Link
US (1) US6924120B1 (enExample)
EP (1) EP1066328B1 (enExample)
JP (1) JP4472870B2 (enExample)
KR (1) KR100554490B1 (enExample)
CN (1) CN1197876C (enExample)
AT (1) ATE408631T1 (enExample)
AU (1) AU765574B2 (enExample)
BR (1) BR9815788B1 (enExample)
CA (1) CA2324513C (enExample)
DE (1) DE69840035D1 (enExample)
DK (1) DK1066328T3 (enExample)
ES (1) ES2317665T3 (enExample)
IL (1) IL138658A0 (enExample)
MX (1) MXPA00009564A (enExample)
WO (1) WO1999050302A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2372199A1 (en) * 1999-05-14 2000-11-23 Medical Research Council Oligomeric chaperone proteins
JP2003502024A (ja) * 1999-05-14 2003-01-21 メディカル リサーチ カウンシル タンパク質骨格および単量体ポリペプチドを多量体化するためのその使用
GB9913437D0 (en) * 1999-06-09 1999-08-11 Medical Res Council Fusion proteins
US20020164712A1 (en) * 2000-12-11 2002-11-07 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence
EP2336164A1 (en) 2005-12-22 2011-06-22 Genentech, Inc. Recombinant production of heparin binding proteins
WO2008008975A2 (en) 2006-07-14 2008-01-17 Genentech, Inc. Refolding of recombinant proteins
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
RU2447149C1 (ru) * 2011-03-24 2012-04-10 Винсорт Менеджемент Инк РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pMSIN4, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД - ПРЕДШЕСТВЕННИК ИНСУЛИНА ЧЕЛОВЕКА, ШТАММ BL21(DE3)/pMSIN4-ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА
CN102628072B (zh) * 2012-03-20 2013-12-04 甘李药业股份有限公司 一种胰岛素的制备方法
CN103439512B (zh) * 2013-07-30 2014-05-07 通化东宝药业股份有限公司 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法
CN112142848A (zh) * 2019-06-26 2020-12-29 中国科学院大连化学物理研究所 一种重组人胰岛素及其纯化制备方法
CN114805610B (zh) * 2022-06-23 2022-10-04 北京惠之衡生物科技有限公司 一种高表达甘精胰岛素前体的重组基因工程菌及其构建方法
CN114805544B (zh) * 2022-06-23 2022-09-09 北京惠之衡生物科技有限公司 一种赖脯胰岛素前体、其重组基因工程菌及其构建方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
NZ199391A (en) 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US5254463A (en) 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4665160A (en) * 1982-03-22 1987-05-12 Genentech, Inc. Novel human growth hormone like protein HGH-V encoded in the human genome
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US5191063A (en) 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US5358857A (en) 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
US5422110A (en) 1991-10-16 1995-06-06 University Of Saskatchewan Enhanced immunogenicity using leukotoxin chimeras
SG46612A1 (en) 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals
US6001604A (en) 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DK0871474T4 (da) * 1994-12-29 2012-01-09 Ferring Int Ct Sa Fremstilling af human insulin
CA2212006A1 (en) * 1995-02-03 1996-08-08 G.D. Searle & Co. Novel c-mpl ligands
US5559128A (en) 1995-04-18 1996-09-24 Merck & Co., Inc. 3-substituted piperidines promote release of growth hormone
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
AUPO394896A0 (en) 1996-11-29 1997-01-02 May, Peter Andrew John Hose mounting bracket
US20020164712A1 (en) * 2000-12-11 2002-11-07 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence
WO2002079251A2 (en) * 2001-04-02 2002-10-10 Novo Nordisk A/S Method for making human insulin precursors

Also Published As

Publication number Publication date
BR9815788B1 (pt) 2013-06-04
ATE408631T1 (de) 2008-10-15
AU6716498A (en) 1999-10-18
EP1066328B1 (en) 2008-09-17
CA2324513A1 (en) 1999-10-07
WO1999050302A1 (en) 1999-10-07
IL138658A0 (en) 2001-10-31
EP1066328A4 (en) 2002-06-05
EP1066328A1 (en) 2001-01-10
US6924120B1 (en) 2005-08-02
DK1066328T3 (da) 2009-02-09
JP4472870B2 (ja) 2010-06-02
JP2004505601A (ja) 2004-02-26
KR20010042383A (ko) 2001-05-25
AU765574B2 (en) 2003-09-25
DE69840035D1 (de) 2008-10-30
MXPA00009564A (es) 2004-03-10
KR100554490B1 (ko) 2006-03-03
ES2317665T3 (es) 2009-04-16
CN1197876C (zh) 2005-04-20
BR9815788A (pt) 2000-11-28
CN1291199A (zh) 2001-04-11

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