CN1184902C - Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method - Google Patents
Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method Download PDFInfo
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- CN1184902C CN1184902C CNB031281117A CN03128111A CN1184902C CN 1184902 C CN1184902 C CN 1184902C CN B031281117 A CNB031281117 A CN B031281117A CN 03128111 A CN03128111 A CN 03128111A CN 1184902 C CN1184902 C CN 1184902C
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Abstract
The present invention discloses function food for protecting the liver, which is prepared by that modern biochemistry technology is used, enzymolysis powder prepared from oleanic acid and silkworm chrysalis is used as principal raw materials, the oleanic acid is extracted by glossy privet fruit, and shaped auxiliary materials, vitamins, etc. are added. The function food has the advantages of specific effective component structure, exact liver protecting function, stable dosage and preparation and obvious preventing and treating effect. The function food has the functions of reducing transaminase and protecting the liver, and has safety, innocuity, and no toxic or side effect by long-term administration.
Description
Technical field
The present invention adopts modern biochemical technology, and oleanolic acid that extracts with Fructus Ligustri Lucidi and the enzymolysis dry powder that makes with Pupa bombycis are as primary raw material, and preparation has the functional food of transaminase lowering, liver protection function.
Background technology
China is the heavy epidemic-stricken area of hepatitis sick district occurred frequently, especially hepatitis B (abbreviation hepatitis B).Hepatitis B is the formidable enemy of harm humans health, also is one of disease of hazardness maximum in China's infectious disease.It is reported, China has hepatitis B virus carriers 1.2 hundred million people (wherein 1/4 people can develop into the chronic hepatitis disease eventually) approximately, about 1,200 ten thousand examples of chronic hepatitis patient, wherein annual because of the dead number of hepatopathy reaches 300,000, and 40% mother who carries hepatitis B virus can give the baby with viral communication.Developing the medicine and the functional food with liver protection effect that hepatitis B there are practical curative effect, is a major issue that brings benefit to the people, and also is medical and health worker's a very urgent strategic task.The functional food that present China has liver protection effect is of less types, these functional food or compound product, or the not bright food of action component, though have liver protection effect through human body or zoopery proof, but the structure of still indeterminate effective ingredient with this function or functional factor and content standard thereof, detection method, can only carry out functional detection, and functional detection is subjected to the influence of factors such as time, funds.As following functional food for protecting liver: compound " Xiling " Medicinal granules is mainly mixed by Herba Rabdosiae Lophanthoidis, Black Ganoderma, Herb Gynostemmae Pentaphylli, Poria, Arillus Longan, Fructus Lycii, Lentinus Edodes, Tremella etc., newborn pigeon health spirits is to be main spice with squab, be equipped with Chinese herbal medicine such as Fructus Lycii, Fructus Jujubae, living Rhizoma Imperatae, make through baking, three grades of immersions, sedimentation and filtrations, it is to be primary raw material with the Misgurni anguillicaudati enzymolysis protein that lucky star board liver protects capsule, though above-mentioned functions food has the effect of the liver protecting and ALT lowering, but ubiquity complicated component, the shortcoming that effective ingredient is not clear can only belong to second filial generation functional food.And the clear and definite functional food of effective ingredient structure and content, promptly third generation functional food is less in the research and development of China, lags behind America and Europe and Japan.Exploitation of China's functional food is compared with advanced international standard for this and there is a big difference, is unfavorable for finding one's way into world market.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide that a kind of effective ingredient structure is clear and definite, liver protection effect is definite, dosage and dosage form is stable, the significant functional food for protecting liver of prevention effect.Its effective ingredient is the known oleanolic acid of chemical constitution (structural formula is as follows), and is equipped with the silkworm chrysalis enzymolyzing powder as nourishing additive agent.Oleanolic acid has stable morphology in this functional food, content standard is clear and definite, and detection method is simple and easy to do.
The present invention realizes by following technical proposals: 1. dried Fructus Ligustri Lucidi is pulverized with pulverizer, used 60～80% alcohol reflux 2～3 times then, each 1～3 hour, filter merging filtrate; 2. add H
2SO
4To the final mass percent concentration be 5～10%, stir, back flow reaction 2～4 hours reclaims ethanol; 3. precipitate is washed till filtrate and becomes neutral with the NaOH solution washing of an amount of pH8～9, and reheat water thorough washing filters, and gets crude product, activated carbon decolorizing; 4. 90～99% ethyl alcohol recrystallizations are 1～2 time, get oleanolic acid; 5. after fresh Antheraea pernyi Geurin Meneville being cleaned, be twisted into the Pupa bombycis slurry, drop in the hydrolysis kettle with meat grinder, the water that adds 2～4 times of quality, stir post-heating to 65～70 ℃ add 1～5% papain then, continue constant temperature and stir 5～48 hours, carry out enzymolysis, after enzymolysis is finished, be warming up to 100～110 ℃, enzyme denaturing sterilization 1～2 hour, after being cooled to room temperature, filter, filtrate changes knockout tower over to, leaves standstill, divide degrease, water places in the concentrating under reduced pressure pot and concentrates, put cold, the impouring storage tank, spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder; 6. oleanolic acid that above-mentioned Fructus Ligustri Lucidi is extracted and the dry powder that carries out enzymolysis with papain that makes with Pupa bombycis is as primary raw material, according to following each component and content (percentage by weight): oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; And contained the adjuvant of starch: flour, starch, Semen Glycines powder, Rhizoma amorphophalli powder, black sesame powder or Semen arachidis hypogaeae powder 0～80%, excipient and thickening agent 0～10%, flavoring agent 0～5%, vitamin 0～2%, food coloring 0～2% and antiseptic 0～1%, the functional food of liver protection effect through being mixed and made into.
Above-mentioned excipient and thickening agent are one of following compositions: sodium carboxymethyl cellulose, agar, gelatin, alginate jelly, pectin; Described flavoring agent is one of following compositions: Sal, sugar, Mel, milk powder, protein sugar, glucide, monosodium glutamate, chicken essence, Fructus Piperis powder, Zanthoxyli Bungeani powder, Fructus Capsici powder, Herba Alii fistulosi, Rhizoma Zingiberis powder, Bulbus Allii powder, Herba Menthae; Described vitamin is: vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K or Citrin; Described food coloring is one of following compositions: carotene, caramel color, chocolate pigment, coffee pigment, Fructus Citri tangerinae pigment; Described edible essence is one of following compositions: lychee flavor, apple essence, flavoring pineapple essence, strawberry essence, honey peach essence, cocoanut flavour, milk flavour, beef flavor, coffee aroma; Described antiseptic is one of following compositions: benzoate, antibacterial peptide, sorbate.
The form of the functional food of liver protection effect of the present invention is tablet, powder, capsule, pill or electuary; Cookies, cake, instant noodles or medicated porridge are stuck with paste.
This finished product taking dose is adult 0.1～100 gram/sky.
According to documents such as " Chinese medicine voluminous dictionaries " record, Fructus Ligustri Lucidi is returned liver, kidney channel, has nourishing the liver and kidney, an effect of the black hair that makes eye bright, and is used for vertigo and tinnitus, soreness of the waist and knees, early whitening of beard and hair, poor vision.Modern pharmacological research proves that the active ingredient oleanolic acid of Fructus Ligustri Lucidi has certain therapeutical effect to experimental acute liver damage, can reduce the activity of glutamate pyruvate transaminase, the clinical treatment that is used for acute and chronic hepatitis, and curative effect is rather good.
Pupa bombycis is not only originated abundant, and has higher nutritive value and health care value.Pupa bombycis is that main body is formed with colloid albumen, and contains fat, glycogen, vitamin etc., and the protein content of bright pupa is up to 17%, and dry product is up to 50%, and fat content is 30%, and wherein unsaturated fatty acid accounts for 70%.The aminoacids complex made from Pupa bombycis is to feeding the effect that rising total serum protein and hemoglobin are arranged with the rat of no nitrilo plinth feedstuff; Experimental hepatitis rat there is the reduction serum glutamic pyruvic transminase, liver-protective effect.The glutamate pyruvate transaminase value that experiment showed, the hepatitis rat of treatment after one month is recovered normally the report of also useful compound silkworm chrysalis treated powder chronic hepatopathy produce effects substantially.
The herbal component of this functional food comprises Fructus Ligustri Lucidi and Pupa bombycis, adopts modern biochemical technology to extract its effective ingredient, be mixed in proportion and add amyloid adjuvant after make.The traditional handicraft of extracting oleanolic acid from Fructus Ligustri Lucidi adopts column chromatography, and this method cost is higher, and is loaded down with trivial details time-consuming, is not suitable for commercial production.The present invention adopts solvent extraction method to extract oleanolic acid, and is with low cost, easy to operation, products obtained therefrom purity height, (more than 90%).Prove that through pharmacology and toxicological experiment this functional food not only has transaminase lowering, the effect of hepatoprotective, and safety non-toxic are taken without any side effects for a long time.This functional food production technology is simple, with low cost, taking convenience, effect are remarkable, has the value of applying.
Following introductory section zoopery result, experiment is undertaken by Disease Prevention Control Center, Hubei Prov.To carbon tetrachloride (CCl
4) cause the inhibitory action of mouse liver injury: get SPF level Kunming kind female mice (20 ± 2g) totally 50 are divided into five groups, 10 every group at random.This functional food is mixed with desired concn, and per os was irritated stomach and was given every day, and basic, normal, high dosage group dosage is respectively 0.15,0.30,0.45g/kg, normal control group and CCl
4Matched group gives the equivalent distilled water.In testing the 31st day with each treated animal fasting overnight 16 hours, CCl
4Matched group and each dosage group give the CCl of 0.15% (v/v) through lumbar injection
4Oil solution 0.1ml/10g, the normal control group gives equivalent commercially available salad oil.Give CCl
4Put to death animal after 24 hours, get glutamate pyruvate transaminase in the hematometry serum (ALT), glutamic oxaloacetic transaminase, GOT (AST).Experimental result shows, CCl
4Control group A LT and AST value obviously raise, each dosage group serum alt and AST value and CCl
4The apparent in view reduction of matched group, difference has utmost point significance.
Table 1 is respectively organized mice serum ALT and AST measured value (x ± s)
Group dosage number of animals ALT AST
(g/kg) (n) (U/L) (U/L)
Normal control group 0 10 46.0 ± 5.5 162.4 ± 35.4
CCl
4Matched group 0 10 221.9 ± 85.8 514.8 ± 86.5
Low dose group 0.15 10 154.4 ± 51.1
*303.9 ± 68.5
*
Middle dosage group 0.30 10 110.3 ± 34.2
*273.0 ± 42.2
*
High dose group 0.45 10 127.1 ± 22.6
*229.5 ± 72.1
*
*With CCl
4Matched group is P＜0.01 relatively
The specific embodiment
Embodiment 1:
The dried Fructus Ligustri Lucidi of 3kg is pulverized with pulverizer, used 70% alcohol reflux 2 times then, each 2 hours.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Precipitate is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, and reheat water thorough washing filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness tap water, deionized water are cleaned with the fresh Antheraea pernyi Geurin Meneville of 3kg, be twisted into the Pupa bombycis slurry, drop in the hydrolysis kettle with meat grinder.The water that adds 4 times of quality, the post-heating to 65 ℃ of stirring adds 1% papain then, continues constant temperature and stirs 24 hours, carries out enzymolysis.After enzymolysis is finished, be warming up to 110 ℃, enzyme denaturing sterilization 2 hours.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the concentrating under reduced pressure pot and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.
By following pharmaceutical formulation: oleanolic acid 150g, silkworm chrysalis enzymolyzing dry powder 200g, starch 1000g, sodium benzoate 0.5g incapsulates by the 0.3g/ grain behind the mixing, packed products again behind the irradiation sterilization.
Embodiment 2:
The dried Fructus Ligustri Lucidi of 5kg is pulverized with pulverizer, used 75% alcohol reflux 3 times then, each 2 hours.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Precipitate is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, and reheat water thorough washing filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness tap water, deionized water are cleaned with the fresh Antheraea pernyi Geurin Meneville of 5kg, be twisted into the Pupa bombycis slurry, drop in the hydrolysis kettle with meat grinder.The water that adds 4 times of quality, the post-heating to 70 ℃ of stirring adds 2% papain then, continues constant temperature and stirs 24 hours, carries out enzymolysis.After enzymolysis is finished, be warming up to 110 ℃, enzyme denaturing sterilization 2h.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the concentrating under reduced pressure pot and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.By following pharmaceutical formulation: oleanolic acid 200g, silkworm chrysalis enzymolyzing dry powder 300g, starch 500g, sodium carboxymethyl cellulose 19.5g, sodium benzoate 0.5g is pressed into the liver-protecting tablet of 0.2g/ sheet behind the mixing.
Embodiment 3:
The dried Fructus Ligustri Lucidi of 5kg is pulverized with pulverizer, used 75% alcohol reflux 3 times then, each 2 hours.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Precipitate is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, and reheat water thorough washing filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness tap water, deionized water are cleaned with the fresh Antheraea pernyi Geurin Meneville of 5kg, be twisted into the Pupa bombycis slurry, drop in the hydrolysis kettle with meat grinder.The water that adds 4 times of quality, the post-heating to 68 ℃ of stirring adds 2% papain then, continues constant temperature and stirs 24h, carries out enzymolysis.After enzymolysis is finished, be warming up to 105 ℃, enzyme denaturing sterilization 2h.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the concentrating under reduced pressure pot and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.Press following pharmaceutical formulation: oleanolic acid 300g, silkworm chrysalis enzymolyzing dry powder 300g, Catergen .5g, sodium benzoate 0.5g makes electuary behind the mixing.
Claims (5)
1. functional food with liver protection effect, it is characterized in that, oleanolic acid that extracts with Fructus Ligustri Lucidi and the dry powder that carries out enzymolysis with papain that makes with Pupa bombycis are as primary raw material, and its content is respectively: oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; And contained the adjuvant of starch: flour, starch, Semen Glycines powder, Rhizoma amorphophalli powder, black sesame powder or Semen arachidis hypogaeae powder 0～80%, excipient and thickening agent 0～10%, flavoring agent 0～5%, vitamin 0～2%, food coloring 0～2% and antiseptic 0～1%, the functional food of liver protection effect through being mixed and made into.
2. according to the functional food of the described liver protection effect of claim 1, it is characterized in that described excipient and thickening agent are one of following compositions: sodium carboxymethyl cellulose, agar, gelatin, alginate jelly, pectin; Described flavoring agent is one of following compositions: Sal, sugar, Mel, milk powder, protein sugar, glucide, monosodium glutamate, chicken essence, Fructus Piperis powder, Zanthoxyli Bungeani powder, Fructus Capsici powder, Herba Alii fistulosi, Rhizoma Zingiberis powder, Bulbus Allii powder, Herba Menthae; Described vitamin is: vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K or Citrin; Described food coloring is one of following compositions: carotene, caramel color, chocolate pigment, coffee pigment, Fructus Citri tangerinae pigment; Described edible essence is one of following compositions: lychee flavor, apple essence, flavoring pineapple essence, strawberry essence, honey peach essence, cocoanut flavour, milk flavour, beef flavor, coffee aroma; Described antiseptic is one of following compositions: benzoate, antibacterial peptide, sorbate.
3. the functional food of the described liver protection effect of claim 1 is characterized in that, the form of its functional food is tablet, powder, capsule, pill or electuary.
4. the functional food of the described liver protection effect of claim 1 is characterized in that, the form of its functional food is that cookies, cake, instant noodles or medicated porridge are stuck with paste.
5. the preparation method of the functional food with liver protection effect of a claim 1, this method comprises the following steps: 1. dried Fructus Ligustri Lucidi to be pulverized with pulverizer, uses 60～80% alcohol reflux 2～3 times then, each 1～3 hour, filters merging filtrate; 2. add H
2SO
4To the final mass percent concentration be 5～10%, stir, back flow reaction 2～4 hours reclaims ethanol; 3. precipitate is washed till filtrate and becomes neutral with the NaOH solution washing of an amount of pH8～9, and reheat water thorough washing filters, and gets crude product, activated carbon decolorizing; 4. 90～99% ethyl alcohol recrystallizations are 1～2 time, get oleanolic acid; 5. after fresh Antheraea pernyi Geurin Meneville being cleaned, be twisted into the Pupa bombycis slurry, drop in the hydrolysis kettle with meat grinder, the water that adds 2～4 times of quality, stir post-heating to 65～70 ℃ add 1～5% papain then, continue constant temperature and stir 5～48 hours, carry out enzymolysis, after enzymolysis is finished, be warming up to 100～110 ℃, enzyme denaturing sterilization 1～2 hour, after being cooled to room temperature, filter, filtrate changes knockout tower over to, leaves standstill, divide degrease, water places in the concentrating under reduced pressure pot and concentrates, put cold, the impouring storage tank, spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder; 6. according to each component and percentage by weight: oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; And amyloid adjuvant 0～80% in addition, excipient and thickening agent 0～10%, flavoring agent 0～5%, vitamin 0～2%, food coloring 0～2%, antiseptic 0～1%, mix homogeneously, product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031281117A CN1184902C (en) | 2003-06-05 | 2003-06-05 | Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method |
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|---|---|---|---|
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| CN1184902C true CN1184902C (en) | 2005-01-19 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278740B (en) * | 2008-05-16 | 2010-12-29 | 孙平贵 | Health care silkworm chrysalis capable of improving immunity of human body |
| CN102715390A (en) * | 2012-05-03 | 2012-10-10 | 湖北炎帝农业科技股份有限公司 | Liver-protecting functional food and preparation method thereof |
| WO2013173972A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for growth in children |
| CN103535610A (en) * | 2013-09-18 | 2014-01-29 | 安徽省蚌埠市花乡食品有限责任公司 | Black peanut noodles capable of dispelling effects of alcohol and protecting liver |
| CN103734722B (en) * | 2013-12-27 | 2015-05-13 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method for silkworm pupa food, silkworm pupa food and application |
| CN105995593A (en) * | 2016-05-24 | 2016-10-12 | 广东汇香源生物科技股份有限公司 | Barbecuing essence and preparation method thereof |
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