JP2002275087A - Antidiabetic medicine and food for preventing diabetes - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antidiabetic medicine which exhibits an excellent antidiabetic action for diabetes or persons who are not diabetic but have high blood glucose levels. SOLUTION: This antidiabetic medicine contains a water extract of the fruit bodies of Auricularia polytricha or its purified product, or the shell powder of Pinctada fucata.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an antidiabetic agent which is a natural product and has an antidiabetic action against diabetes, and to a preventive food containing the antidiabetic agent.

[0002]

2. Description of the Related Art With the development of the economy, dietary habits have improved, and the intake of sweeteners and carbohydrates, including soft drinks, has exceeded the required amount, and it cannot be said that the disease is non-insulin-dependent diabetes or diabetes. Even so, many people have blood sugar levels that are outside the normal range. Diabetes has many complications (retinopathy,
Nephropathy, neuropathy, cerebral infarction, myocardial infarction, cataract)
Social loss is a serious problem. However, improving eating habits is difficult in modern environments. Also,
There are various antidiabetic agents, but in general, chemically synthesized products have high effects but also have side effects, so that there are many problems in long-term administration.

On the other hand, among natural products which have been used for food for many years and whose safety to the human body has been confirmed, jellyfish (Auricularia auricula-judae) is known to be administered to diabetic rats induced by administering streptozotocin during the neonatal period. On the other hand, an improving effect on blood glucose level and insulin secretion has been recognized (Journal of the Japan Nutrition and Food Society, 51
Vol. 3, No. 129-133, 1998).

The pearl oyster (Pinctada fucata) is
Produced in large quantities for pearl farming. As a drug substance utilizing the pearl oyster, a substance obtained by heating a pearl is known (JP-A-1-19991).
2).

[0005]

However, Auricularia polytricha resembling a jellyfish
Is inferior to jellyfish in terms of quality when used as food, and has about half the crude protein content in terms of ingredients, belongs to the same jellyfish family and is edible, but is treated as a natural product with a different composition I have. For this reason, there was a problem that it was unclear whether or not an antidiabetic agent could be obtained from A. jellyfish. The pearl oyster (Pinctada fucata) has a problem that its effective utilization after pearl collection has not been sufficiently performed, and it is unclear whether or not it has an effect as an antidiabetic agent. The present invention has been made in order to address such a problem, and has excellent anti-diabetic effects on people with high blood sugar levels, although not diabetes or diabetes. An object of the present invention is to provide an antidiabetic agent obtained from shellfish, or a food for preventing diabetes containing the same.

[0006]

The anti-diabetic agent of the present invention comprises:
It is characterized by containing an aqueous extract of the fruit body of Auricularia jellyfish (Auricularia polytricha).

[0007] Other antidiabetic agents include Auri jellyfish (Auri
water-soluble polysaccharide derived from an aqueous extract of the fruiting body of C. cularia polytricha). Also, the water-soluble polysaccharide is Auricularia polytric
It is characterized in that it is a precipitate obtained by precipitating a water extract of the fruiting body of ha) with a water-soluble organic solvent or a mixed solvent of a water-soluble organic solvent and water.

Another anti-diabetic agent is pearl oyster (Pinc
tada fucata) shell powder.

[0009] The diabetes preventive food of the present invention is characterized in that the above-mentioned antidiabetic agent is incorporated in the food alone or in combination.

The present inventors have screened and examined natural products which have been used for food for many years and have been confirmed to be safe for the human body. As a result, the water-soluble polysaccharide was found to be a component obtained from the fruiting bodies of the jellyfish jellyfish. The present inventors have found that the water-soluble polysaccharide has an anti-diabetic effect on diabetes, particularly non-insulin-dependent diabetes, in a larger amount than the component obtained from Rhododendron. In addition, the inventors have found that shell powder of pearl oyster (Pinctadafucata) has an anti-diabetic effect. The present invention is based on these findings.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The fruiting bodies of the jellyfish jellyfish that can be used in the present invention may be cultured fruiting bodies, naturally occurring fruiting bodies, or a mixture of these fruiting bodies. As the fruiting body, either a commercially available dried product or a collected raw product can be used. The fruiting body of the jellyfish jellyfish can be extracted with water. The extraction temperature is preferable because a temperature higher than room temperature can be efficiently extracted,
The preferred extraction temperature is 50-100 ° C. The extraction time varies depending on the temperature, etc., but it is preferable that the extraction time is about 30 minutes to 5 hours because the extraction can be performed efficiently. If the extraction efficiency is to be further increased, repeat the extraction several times. The amount of water to be added varies depending on the conditions of the above extraction, but the amount of water to be extracted is preferably 10 to 1000 parts by weight per 1 part by weight of the fruit body of the mushroom. If the amount is less than 10 parts by weight, extraction of the active ingredient is insufficient, and if it exceeds 1000 parts by weight, concentration and drying of the extract become difficult. The extract can be concentrated or dried by a known method and used as an antidiabetic agent or its preventive food.

[0012] The water extract of the fruit body of the Asteraceae jellyfish can be further separated and purified to obtain a substance having better antidiabetic action. This substance was found to be a high molecular weight water-soluble polysaccharide contained in the water extract. In the separation and purification of the water extract, it is preferable to purify the aqueous extract by a method for removing low molecular weight substances. As a method for removing low molecular weight substances, a known method such as a method of purifying using a water-soluble organic solvent, a method of fractionating using column chromatography, an electrophoresis method, an ultracentrifugation method, or a dialysis method may be used. it can. Among these, the method of purification using a water-soluble organic solvent is a highly useful and preferable method because the target substance can be obtained by a simple method.

The method for purification using a water-soluble organic solvent is as follows:
A water-soluble organic solvent or a mixed solvent of a water-soluble organic solvent and water is added to an aqueous extract of the fruiting body of the jellyfish jellyfish to precipitate high molecular weight substances dissolved in the aqueous extract. This is a method for separation and purification. Examples of the water-soluble organic solvent include lower monohydric alcohols such as methanol, ethanol, butanol, and isopropyl alcohol, polyhydric alcohols such as ethylene glycol and glycerin, and water-soluble ketones such as acetone. Of these, ethanol is most preferred. This is because the separated and purified substance becomes an antidiabetic agent or a raw material for preventing diabetes and is eaten by humans. As a specific separation and purification method, ethanol is added to an aqueous extract of the fruiting body of the mushroom jellyfish, so that the total ethanol concentration is 50% by weight or more. This is stirred, and the precipitate is collected using a known method such as decantation or centrifugation. If the used water-soluble organic solvent is of a type that cannot be drunk, it is necessary to remove the organic solvent sufficiently, but in the case of ethanol, there is no problem if it remains depending on the application. Alternatively, a water removing step is performed.

The pearl oyster (Pinctada f) which can be used in the present invention
The shell powder of ucata) is a powder obtained by crushing and refining a shell, particularly a nacre, after collecting pearls. This powder has substantially the same composition as the pearl powder used in Chinese herbal medicine, and contains pearl protein (conchiolin). The shell powder can be used as an antidiabetic agent or its preventive food in powder form or as an aqueous dispersion.

The extract, purified product, pearl oyster shell powder, or a mixture thereof of the fruit body of the genus Asteraceae can be mixed with other necessary raw materials to obtain an antidiabetic preventive food. The antidiabetic agent of the present invention has an effect of suppressing the absorption of carbohydrates contained in foods and preventing a rapid rise in blood sugar. Useful as food. As a health food, it can be suitably blended with tablets, granules, powdered soups, and other favorite products such as tea.

[0016]

EXAMPLES Example 1 A dried product of the fruit body of the Chinese jellyfish jellyfish was pulverized with a mixer into powder. The powder was added with 20 times by weight of water, and extracted for 3 hours while stirring under heating using a boiling water bath. After that, centrifugation removes the extraction residue,
The first extract was obtained. The same extraction treatment as above was repeated three more times on the extraction residue to obtain second to fourth extraction liquids. The first to fourth extraction liquids were combined, freeze-dried under vacuum, and extracted with hot water to obtain a water extract dried powder of the fruit body of the jellyfish jellyfish. The yield of the obtained dry powder of the water extract was 34% by weight based on the body of the dried jellyfish.

The water extract dried powder obtained in Example 1 was subjected to the following antidiabetic action test. KK- ^Ay / TaJcl mice (hereinafter referred to as KK- ^Ay mice) were used as non-insulin-dependent diabetes model animals. KK
-A ^y mice are spontaneously non-insulin dependent diabetes mellitus model animal exhibiting a condition similar to human non-insulin dependent diabetes mellitus. This mouse only needs to feed normal food,
It is known that mice have insulin resistance as compared with normal mice, and exhibit characteristic symptoms such as obesity, overeating, polydipsia, high blood sugar, high urinary glucose, and decreased glucose tolerance. Four-week-old male KK- ^Ay mice (Clear Japan Co., Ltd .; same hereafter) were fed a basic diet and preliminarily reared for 18 days before use in the experiment. The basic feed was composed of 20.0% by weight of casein, 0.3% by weight of DL-methionine, 45.0% by weight of α-corn starch, 20.0% by weight of sucrose, and a mineral mixture (AIN7).
6) 3.5% by weight, vitamin mixture (AIN76) 1.0% by weight, choline bitartrate 0.2% by weight, corn oil 5.0% by weight, and cellulose 5.0% by weight.

[0018] One group of mice based on body weight and blood glucose level
Five animals were divided into two groups (administration group and control group), each of which was bred on experimental diet for 6 weeks. The experimental feed in the treatment group was adjusted from 5.0% by weight to 2.0% of cellulose in the basic feed.
In this case, the feed was reduced to 3.0% by weight, and instead the dry powder of the water extract obtained in Example 1 was added in an amount of 3.0% by weight. Rearing environment is room temperature 2
The animals were individually raised in a stainless steel suspension cage with a mesh bottom at a temperature of 3 to 25 ° C., a relative humidity of 50 to 60%, and a light / dark cycle of 12 hours. Food and drinking water were available ad libitum during the experiment. However, during the measurement described below, feed intake was restricted as described in each measurement method.

During the experiment, mice were measured for body weight, feed intake, water intake, fasting blood glucose, postprandial blood glucose and urinary glucose. The measurement results are expressed as [mean ± standard error] using the average value and standard error of 5 animals in each group. In the table, numerical values marked with an asterisk [*] indicate significant differences of p <0.05 with respect to the control in the Student-t test, respectively.

(1) Measurement of Body Weight, Feed Intake, and Water Intake Mice were weighed once a week, and feed intake and water intake were measured daily. Table 1 shows the body weight at the start of the experiment, the body weight at the end of the experiment, the average feed intake, and the average water intake.

(2) Measurement of Fasting Blood Glucose Level The day before administration of experimental feed (day 0) and on days 7 and 14 of administration
After 21 days, 28 days and 35 days, the mice were fasted for 5 hours, blood was collected from the tail vein under anesthesia, and immediately centrifuged to collect plasma. Glucose concentration in plasma (blood sugar level)
Was measured using glucose CII-Test Wako (Wako Pure Chemical Industries, Ltd .; the same applies hereinafter). Table 1 shows the results.
Shown in

(3) Measurement of postprandial blood glucose level After breeding the mice on the experimental diet for 5 weeks, they were fasted for 5 hours and ingested each experimental diet corresponding to 2 g / kg (body weight) of carbohydrate for 30 minutes. . One hour and two hours after the meal, blood was collected from the tail vein under anesthesia and immediately centrifuged to collect plasma. Glucose concentration (blood sugar level) in plasma was measured using Glucose CII-Test Wako. Table 1 shows the results.

(4) Measurement of Urine Sugar Level After the mice were bred for 4 weeks on the experimental feed, urine was collected for 24 hours, and the amount of glucose excreted in the urine was determined. The amount of glucose in urine (urine sugar level) was measured using Glucose CII-Test Wako. Table 1 shows the results.

[0024]

[Table 1]

To summarize the experimental results in Table 1, the hot-water extract of the fruit body of Asterella jellyfish improves overeating, polydipsia, suppresses fasting blood glucose elevation, and increases postprandial elevation of insulin-independent diabetes. It was found to have an effect of suppressing blood sugar and an effect of reducing urinary glucose excretion.

Example 2 A four-fold amount (V / V) was added to the extract before freeze-drying of Example 1.
95% ethanol was added and the resulting precipitate was collected by centrifugation. The precipitate was dissolved in water, placed in a dialysis membrane (trade name: Cellulose tube 30/32, Sanko Junyaku Co., Ltd.), and dialyzed in running water for 48 hours. The remaining solution (the solution inside the dialysis membrane) was freeze-dried in vacuo to obtain a precipitate separated and purified with ethanol. This precipitate was a water-soluble polysaccharide. The yield of this water-soluble polysaccharide was 24% by weight based on the fruit body of the dried jellyfish jellyfish.

The water-soluble polysaccharide obtained in Example 2 was subjected to the following antidiabetic activity test. 4-week-old male KK-A
^Y mice were fed the basic diet used in the test of Example 1,
After 7 days of preliminary breeding, they were used for experiments.

One group of mice based on body weight and blood glucose level
Eight animals were divided into two groups (administration group and control group), and reared on the experimental feed for 8 weeks in the same breeding environment as in Example 1. The experimental feed of the administration group was a feed in which the blending ratio of cellulose in the basic feed was reduced from 5.0% by weight to 3.0% by weight, and 2.0% by weight of the water-soluble polysaccharide obtained in Example 2 was added instead. The experimental diet of the group is the same as the basic diet.

During the experimental period, the body weight, feed intake, water intake, fasting blood glucose level, urine glucose level, non-fasting blood glucose level and small intestinal disaccharide hydrolase activity of the mice were measured, and the glucose tolerance test and insulin tolerance were measured. The test was performed. The measurement results were 8 for each group.
Using the average value and the standard error of each animal, it is expressed as [mean ± standard error]. In the table, numerical values marked with an asterisk [*] are Stu
The results show a significant difference of p <0.05 with respect to the control by the dent-t test.

The body weight, feed intake and water intake were measured in the same manner as in Example 1. In addition, the fasting blood glucose level was measured by the same method as in Example 1 on the day before the administration of the experimental feed (day 0) and on days 14, 28 and 49 after administration. Table 2 shows the results.

After breeding the mice on the experimental diet for 4 weeks,
The urinary sugar level was measured in the same manner as in Example 1. Table 2 shows the results.

(5) Glucose Tolerance Test After breeding mice for 5 weeks on an experimental feed, they were fasted for 20 hours, and glucose was intraperitoneally administered at a dose of 1 g / kg (body weight) immediately before glucose administration. And administration 30 minutes, 60
After 120 minutes and 120 minutes, blood was collected from the tail vein under anesthesia, and immediately centrifuged to collect plasma. Glucose concentration (blood sugar level) in plasma was measured using Glucose CII-Test Wako. Table 2 shows the results. The sum of the blood glucose levels immediately before and 30 minutes, 60 minutes, and 120 minutes after the administration of glucose was defined as the total blood glucose level. The results are shown in Table 2.

(6) Insulin Tolerance Test After breeding mice for 7 weeks on the experimental feed, they were fasted for 24 hours, and insulin (Nippon Eli Lilly Co., Ltd.)
U / kg (body weight) was administered subcutaneously, blood was collected from the tail vein under anesthesia immediately before insulin administration, and at 30, 60, and 90 minutes after administration, and immediately centrifuged to collect plasma. The glucose concentration (blood sugar level) in plasma
It measured using II-Test Wako. Table 2 shows the results.

(7) Measurement of non-fasting blood glucose level After breeding mice for 8 weeks on an experimental feed, blood was collected by decapitation under non-fasting and non-anesthesia, and blood was immediately collected by centrifugation. The glucose concentration (blood sugar level) in the serum was measured using Glucose CII-Test Wako. Table 2 shows the results.
Shown in

Diabetes causes very large changes in intestinal function. It is known that disaccharide hydrolase activity in the small intestine is increased, digestion is accelerated, and absorption of carbohydrates into the bloodstream is increased. For this reason, the following examination was carried out to clarify the inhibitory effect of the extract of the fruit body of the mushroom jellyfish according to the present invention on the intestinal disaccharide hydrolase activity of KK- ^Ay mice.

(8) Measurement of Intestinal Disaccharide Hydrolase Activity The mice were bred for 8 weeks on an experimental diet, then decapitated and sacrificed under non-fasting and non-anesthetized conditions, and the entire small intestine was removed in a usual manner.
The mucous membrane of the inner wall of the jejunum of the mouse is collected, homogenized in 10 mM potassium phosphate buffer (pH 7.0), and the precipitate is removed by centrifugation (2,000 rpm, 10 minutes, 4 ° C). A liquid was obtained. This crude enzyme solution was subjected to the following measurements of sucrase, maltase, and isomaltase activities.

The prepared crude enzyme solution was used as a substrate solution at 0.1 M
2 dissolved in sodium maleate buffer (pH 5.8)
8mM sucrose or 28mM maltose or
After adding 2.8 mM isomaltose and reacting at 37 ° C for 1 hour, the reaction was stopped by heating in a boiling water bath for 3 minutes, and the amount of glucose produced by the enzyme reaction was measured using a glucose CII-Test Wako did. Table 2 shows the obtained results. Disaccharide hydrolase activity is the amount of enzyme hydrolyzed in 1 hour per 1 mg of protein in crude enzyme solution.
Expressed in mo1 units. The protein amount of the crude enzyme solution was measured by the Lowry method using bovine serum albumin as a standard.

[0038]

[Table 2]

To summarize the experimental results in Table 2, the water-soluble polysaccharides of the fruiting bodies of the jellyfish jellyfish have the effect of improving overeating, polydipsia, suppressing the increase in fasting blood glucose, and urinary glucose against non-insulin-dependent diabetes mellitus. It was found to have an effect of reducing excretion, an effect of improving glucose tolerance, an effect of improving insulin resistance, and an effect of suppressing intestinal disaccharide hydrolase activity.

KK-A^yOnset of diabetes in mice
From the early stage of the present invention
When a feed containing the extract of
The dose of jellyfish hot water extract is 3%,
Even at a dose of 2%, it sufficiently suppresses the progress of diabetes
It became clear that it could be done. Also, jellyfish jellyfish
KK-A administered with hot water extract or water-soluble polysaccharide ^y
When the mice were observed, the mice were observed during and after administration.
Showed no side effects and no acute toxicity was observed. What
Because the sensitivity of humans is higher than that of mice,
Furthermore, the extract of the fruiting body of the jellyfish jellyfish according to the present invention
When administered to humans, even if the dose is less than the above
Can be inferred to be effective.

Example 3 As a shell powder of pearl oyster (Pinctada fucata), a commercially available powder (Betacalc, manufactured by Mikimoto Pharmaceutical Co., Ltd.) was used for the following antidiabetic test. KK- ^Ay mice were used as non-insulin dependent diabetes model animals. Four-week-old male KK- ^Ay mice (CLEA Japan, same hereafter) were fed with a basic diet and preliminarily reared for 7 days, and then two groups of mice showing urinary glucose were determined based on body weight and blood glucose level. Control group: n = 9; Betacalc group: n = 11
). In the control group, the basic feed (control feed) used in Example 1 was subsequently given, and in the beta-calc group, the blending ratio of cellulose in the basic feed was reduced from 5.0% by weight to 2.0% by weight, and instead, 3.0% by weight of beta-calc was added. The feed was fed and raised. The breeding environment is room temperature 23-25 ℃,
They were individually housed in stainless steel hanging cages with a mesh bottom under the condition of a relative temperature of 50 to 60%. Food and drinking water were available ad libitum during the experiment. However, during the measurement described below, feed intake was restricted as described in each measurement method.

During the experiment, the body weight, feed intake, drinking water, 6-hour fasting blood glucose level, and urinary glucose excretion of the mice were measured. The measurement results were obtained for each experimental group (control group: n = 9;
The average value and the standard error of the beta-calc group: n = 11) are used to represent [average value ± standard error]. In the table, the numerical value with an asterisk [*] indicates a value that was compared with the control in the Student-t test.
It shows the significant difference of p <0.05, respectively.

(1) Measurement of Body Weight, Feed Intake, and Drinking Water Mice were weighed weekly, and feed intake was measured daily. Water consumption was measured at 5 weeks and 4 weeks after administration of experimental feed.
Performed in the week. Water consumption was measured every 48 hours every week, and daily amount was calculated. Table 3 shows the results.

(2) Measurement of 6-hour fasting blood glucose level The measurement of 6-hour fasting blood glucose level was performed the day before the administration of the experimental feed and 2, 4, and 6 weeks after the administration. After the mice were fasted for 6 hours, blood was collected from the tail vein under anesthesia and immediately centrifuged to separate the plasma. Glucose concentration (blood sugar level) in plasma was measured using Glucose CII-Test Wako (Wako Pure Chemical Industries, Ltd.). Table 3 shows the results.

(4) Measurement of urinary glucose excretion After the experimental feed was administered for 6 weeks, urine was collected from the mice for 24 hours, and the amount of glucose excreted in the urine was determined. The amount of glucose in urine (urine sugar level) was measured using glucose CII-Test Wako. Table 3 shows the results.

[0046]

[Table 3]

Summarizing the results in Table 3, it was found that the pearl oyster shell powder had an effect of reducing water consumption, an effect of suppressing an increase in blood sugar, and an effect of decreasing urinary glucose excretion.

An anti-diabetic food can be obtained by blending the extract of the fruit body of the mushroom jellyfish, or the shell powder of the pearl oyster, alone or as a mixture with foods. Hereinafter, specific examples of the diabetes prevention food will be described.

Example 4 Blending ratio of health food 1 (tablet) A: 50% by weight of extract of the fruiting body of Asteraceae jellyfish B: 25% by weight of dextrin C: 22.5% by weight of crystalline cellulose D: 2% by weight of sucrose fatty acid ester E: 0.5% by weight of starch Each component of AC is weighed, and the component E is granulated as a binder solution by a fluidized bed granulator. Thereafter, the D component is added and uniformly mixed, and made into tablets by a tableting machine.

Example 5 Mixing ratio of health food 2 (granules) A: extract of the fruiting body of Asteraceae jellyfish 50% by weight B: erythritol 30% by weight C: crystalline cellulose 18% by weight D: sucrose fatty acid ester 2% by weight E: 0.5% by weight of starch Each component of AC is weighed, and the component E is granulated as a binder solution by a fluidized bed granulator. Thereafter, the D component is added and uniformly mixed to obtain granules.

Example 6 Mixing Ratio of Soup 1 (Powdered Soup) A: 30 wt% extract of the fruit body of the jellyfish jellyfish B: 25 wt% of soybean protein C: powdered fat 13 wt% D: broth 11 wt% E: Salt 6% by weight F: Thickener (guar gum) 5% by weight G: Milk protein 5% by weight H: Seasoning (amino acid etc.) 3% by weight I: Flavor 2% by weight Weigh each component of A to I and uniformly Mix to form a powdered soup.

Example 7 Mixing ratio of tea 1 (powder type) A: 30 wt% extract of the fruiting body of Asteraceae jellyfish B: 25 wt% of banaba tea C: 25 wt% of barley tea D: 20 wt% of black tea Each component of D is weighed and uniformly mixed to obtain a powder.

Example 8 Mixing ratio of health food 3 (tablet) A: 50% by weight of pearl oyster shell powder B: 25% by weight of dextrin C: 22.5% by weight of crystalline cellulose D: 2% by weight of sucrose fatty acid ester E: Starch 0.5 Each component of weight% AC is weighed, and the component E is granulated as a binder solution by a fluid bed granulator. Thereafter, the D component is added and uniformly mixed, and made into tablets by a tableting machine.

Example 9 Mixing ratio of health food 4 (granules) A: pearl oyster shell powder 50% by weight B: erythritol 30% by weight C: crystalline cellulose 17.5% by weight D: sucrose fatty acid ester 2% by weight E: starch 0.5 Each component of weight% AC is weighed, and the component E is granulated as a binder solution by a fluid bed granulator. Thereafter, the D component is added and uniformly mixed to obtain granules.

Example 10 Mixing ratio of soup 2 (powder soup) A: pearl oyster shell powder 30% by weight B: soy protein 25% by weight C: powdered fat 13% by weight D: bouillon 11% by weight E: salt 6% by weight F: Thickener (guar gum) 5% by weight G: Milk protein 5% by weight H: Seasoning (amino acids, etc.) 3% by weight I: Flavor 2% by weight A to I components are weighed and uniformly mixed to powder Soup.

Example 11 Mixing ratio of tea 2 (powder type) A: pearl oyster shell powder 30% by weight B: banaba tea 25% by weight C: pigeon barley tea 25% by weight D: black tea 20% by weight Each component of A to D Are weighed and uniformly mixed to obtain a powder.

Example 12 Mixing ratio of health food 5 (tablet) A: 25% by weight of an extract of the fruit body of a jellyfish jellyfish B: 25% by weight of pearl oyster shell powder 25% by weight of dextrin D: 22.5% by weight of crystalline cellulose E: 2% by weight of sucrose fatty acid ester F: 0.5% by weight of starch Each of the components A to D is weighed, and the F component is granulated as a binder solution by a fluid bed granulator. Thereafter, the E component is added and uniformly mixed, and made into tablets by a tableting machine.

Example 13 Blending ratio of health food 6 (granules) A: Extract of the fruit body of Asteraceae jellyfish 25% by weight B: Pearl oyster shell powder 25% by weight C: Erythritol 30% by weight D: Crystalline cellulose 17.5% by weight E: 2% by weight of sucrose fatty acid ester F: 0.5% by weight of starch Each of the components A to D is weighed, and the F component is granulated as a binder solution by a fluid bed granulator. Thereafter, the E component is added and uniformly mixed to obtain granules.

Example 14 Mixing Ratio of Soup 3 (Powdered Soup) A: Extract of the fruit body of Asteraceae jellyfish 15% by weight B: Pearl oyster shell powder 15% by weight C: Soy protein 25% by weight D: Powdered fat and oil 13% by weight % E: Bouillon 11% by weight F: Salt 6% by weight G: Thickener (guar gum) 5% by weight H: Milk protein 5% by weight I: Seasonings (amino acids, etc.) 3% by weight J: Flavor 2% by weight A ~ Each component of J is weighed and uniformly mixed to obtain a powdered soup.

Example 15 Mixing Ratio of Tea 3 (Powder Type) A: Extract of the fruit body of Asteraceae jellyfish 15% by weight B: Akoya shell powder 15% by weight C: Banaba tea 25% by weight D: Pigeon barley tea 25% by weight % E: Black tea 20% by weight Each of the components A to E is weighed and uniformly mixed to obtain a powder.

Each of the anti-diabetic foods of Examples 4 to 15 had the same taste and texture as the foods not containing the extract of the fruit body of Arauki jellyfish and the pearl oyster shell powder. Also, from the experimental results of Examples 1 to 3, foods containing these are excellent as diabetes prevention foods.

[0062]

EFFECT OF THE INVENTION The anti-diabetic agent containing the water extract of the fruit body of the mushroom jellyfish or the pearl oyster shell powder according to the present invention and the isolated and purified anti-diabetic agent are effective in improving overeating and heavy drinking against diabetes. It has the effect of suppressing fasting blood glucose elevation, suppressing postprandial hyperglycemia, reducing urinary glucose excretion, improving glucose tolerance, improving insulin resistance, and suppressing intestinal disaccharide hydrolase activity. Therefore, it can be used not only for treating diabetes but also for preventing or suppressing the onset of diabetes. Very useful for diabetics and those in the reserve. In addition, since the raw material is an extract derived from a natural substance used as food, it is highly safe for the human body.

Since the anti-diabetic agent of the present invention contains the above-mentioned anti-diabetic agent alone or as a mixture in the food, it is highly safe for the human body and not only treats diabetes but also prevents or suppresses the onset of diabetes. Useful for

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A23L 2/38 A61K 35/56 A61K 35/56 A61P 3/10 A61P 3/10 A23L 2/00 F F-term (Reference) 4B017 LC03 LG15 LK17 4B018 MD75 MD82 ME03 MF01 4B036 LC06 LF01 LH34 LH37 4C087 AA01 AA02 BB16 MA43 MA52 NA14 ZC35 4C088 AA03 AC17 BA09 CA08 CA19 MA43 MA52 NA14 ZC35

Claims (5)

[Claims] 1. A jellyfish (Auricularia polytric)
ha) An antidiabetic agent containing an aqueous extract of fruiting bodies. 2. A jellyfish (Auricularia polytric)
An antidiabetic agent containing a water-soluble polysaccharide derived from the water extract of the fruiting body of ha). 3. The water-soluble polysaccharide is a precipitate obtained by adding a water-soluble organic solvent or a mixed solvent of a water-soluble organic solvent and water to an aqueous extract of a fruit body of Auricularia polytricha. 3. The method according to claim 2, wherein
The antidiabetic agent according to the above. 4. An antidiabetic agent containing shell powder of pearl oyster (Pinctada fucata). 5. A diabetes-preventive food, wherein the antidiabetic agent according to any one of claims 1 to 4 is incorporated in the food.