CN110106216A - A kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc - Google Patents
A kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc Download PDFInfo
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- CN110106216A CN110106216A CN201910442443.9A CN201910442443A CN110106216A CN 110106216 A CN110106216 A CN 110106216A CN 201910442443 A CN201910442443 A CN 201910442443A CN 110106216 A CN110106216 A CN 110106216A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Abstract
The present invention provides a kind of extracting methods of Polysaccharide from Auricularia Polytricha Sacc, the extracting method are as follows: be added to the water Uricularia polytricha, then complex enzyme is added, it stirs evenly, pH value is adjusted to 6.0,80min is digested under conditions of temperature is 60 DEG C, yeast is added after enzyme deactivation, it is 0.15MPa~0.2MPa in fermentation pressure, fermentation temperature ferments 20h under conditions of being 40 DEG C, then under conditions of temperature is 60 DEG C after reflux extraction 120min, centrifugation, after taking supernatant concentration to adjust pH value, alcohol analysis, refrigerated centrifuge, vacuum decompression drying, Polysaccharide from Auricularia Polytricha Sacc is obtained.The present invention utilizes complex enzyme zymohydrolysis method, the insoluble fibre of Uricularia polytricha, colloid and vegetable protein are digested, it can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion is ruptured by yeast fermentation again, be conducive to the release of Polysaccharide from Auricularia Polytricha Sacc, be conducive to sufficiently extract the polysaccharide component in Uricularia polytricha, the present invention has more industrialization production advantage, while reducing energy consumption and environmental pollution, lays the foundation for the comprehensive exploitation and deep processing of Polysaccharide from Auricularia Polytricha Sacc.
Description
Technical field
The invention belongs to technical field of plant extraction, and in particular to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc.
Background technique
Uricularia polytricha (Auricularia polytricha Sacc) is also known as Yellow-back fungus, thick agaric etc., belongs to basidiomycetes
Guiding principle, Auriculariale, Auriculariaceae, the Uricularia polytricha kind in Auricularia.Wild Uricularia polytricha is mainly distributed on the low sea such as Sichuan, Yunnan, Fujian
Pull out warm and moist mountain area.Its scale, the regional plantation place of production are concentrated mainly on Sichuan Province Shifang City and Fujian Province partial region.
Uricularia polytricha fructification colloid cunning is tough, and the fructification back side is full of nutrition rich in villus, containing Auricularia polysaccharide, ergosterol, amino acid,
Liposome, cellulose, carrotene, vitamin A, B1、B2And various inorganic elements potassium, sodium, calcium, magnesium, iron, copper, zinc, manganese, phosphorus etc.
Nutritional ingredient is known as the laudatory title of " black health-care food " and " jellyfish on tree ", fills blood, is blood viscosity lowering, reducing blood lipid, clear
Lung is moisturized, washs stomach and intestine, defaecation decompression, the inducing decomposition of calculi, antithrombotic, prevention of arterial atherosis, apoplexy and myocardial infarction clearly and other effects.
A variety of drug effects of Uricularia polytricha and its polysaccharide rich in are closely related.Polysaccharide from Auricularia Polytricha Sacc belongs to acid heteroglycan, is by five kinds
Monosaccharide and glucuronic acid composition.Auricularia polysaccharide has hemostasis, anticoagulation and reducing blood lipid, anti-aging, improves cardiac function, is immune
And anti-tumor function, to body cell have protection etc. a variety of effects.
Polysaccharide from Auricularia Polytricha Sacc extraction is the widely applied basis of polysaccharide therefore to obtain efficient Polysaccharide from Auricularia Polytricha Sacc extractive technique outstanding
It is important.Unified Polysaccharide from Auricularia Polytricha Sacc industrialization extraction process not yet domestic at present, is mostly such technique with leach extraction method
Time-consuming, energy consumption, inefficient and be affected to environment, industrialized production difficulty is larger.
Summary of the invention
Technical problem to be solved by the present invention lies in view of the above shortcomings of the prior art, provide a kind of Polysaccharide from Auricularia Polytricha Sacc
Extracting method, the extracting method utilize complex enzyme zymohydrolysis method, by the insoluble fibre of Uricularia polytricha, colloid and plant rennet
Solution, then can make by yeast fermentation that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion rupture, it is more to be conducive to Uricularia polytricha
The release of sugar is conducive to sufficiently extract the polysaccharide component in Uricularia polytricha, and the present invention has more industrialization production advantage, reduces simultaneously
Energy consumption and environmental pollution lay the foundation for the comprehensive exploitation and deep processing of Polysaccharide from Auricularia Polytricha Sacc.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc, it should
Extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being (1.4~1.6): 1:(1.9~2.1).
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under conditions of temperature is 90 DEG C~100 DEG C, obtains enzymolysis liquid；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.15MPa~0.2MPa, fermentation temperature in fermentation pressure
Ferment 20h under conditions of being 40 DEG C, obtains fermentation liquid；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 6.0~8.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 2h~4h,
Ethyl alcohol is recycled, then revolving speed is centrifuged 10min under conditions of being 3000rpm, in temperature is 1 DEG C~2 DEG C by obtained sediment
Under the conditions of the dry 2h~4h of vacuum decompression, obtain Polysaccharide from Auricularia Polytricha Sacc.
Preferably, the mass ratio of complex enzyme cellulase described in S1, neutral proteinase and pectase is 1.5:1:2.
Preferably, the enzyme activity amount of complex enzyme cellulase described in S1, neutral proteinase and pectase is respectively
20000U/g, 30000U/g and 30000U/g.
Preferably, the mass ratio of Uricularia polytricha described in S1, water and complex enzyme is 1:35:0.1.
Preferably, the mass ratio of enzymolysis liquid described in S3 and yeast is 100:1.4.
Preferably, the volume ratio of concentrate described in S4 and ethanol solution is 1:4.
Preferably, concentrate described in S4 is the 1/4 of the supernatant volume.
Compared with the prior art, the present invention has the following advantages:
The present invention utilizes complex enzyme zymohydrolysis method, and the insoluble fibre of Uricularia polytricha, colloid and vegetable protein are digested, then leads to
Crossing yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion rupture, be conducive to releasing for Polysaccharide from Auricularia Polytricha Sacc
Put, be conducive to sufficiently extract Uricularia polytricha in polysaccharide component, the present invention have more industrialization production advantage, while reduce energy consumption and
Environmental pollution lays the foundation for the comprehensive exploitation and deep processing of Polysaccharide from Auricularia Polytricha Sacc.Complex enzyme enzyme in the present invention selects cellulose
Enzyme, pectase and neutral proteinase, cellulose, pectin and vegetable protein rich in Uricularia polytricha, using cellulase and
The broken wall ability of pectase, hydrocellulose and pectin, promote the dissolution of effective component intracellular；Neutral proteinase can hydrolyze hair
Protein on fungus mycelium wall is conducive to mycelia wall and cracks, and can hydrolyze the protein connecting with polysaccharide, conducive to releasing for polysaccharide
It puts.
Below with reference to embodiment, invention is further described in detail.
Specific embodiment
Embodiment 1
The extracting method of the Polysaccharide from Auricularia Polytricha Sacc of the present embodiment, the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being 1.5:1:2；The enzyme activity amount of the complex enzyme cellulase, neutral proteinase and pectase be respectively 20000U/g,
30000U/g and 30000U/g；The mass ratio of the Uricularia polytricha, water and complex enzyme is 1:35:0.1；
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under conditions of temperature is 100 DEG C, obtains enzymolysis liquid；Pass through insoluble fibre, colloid and the plant in enzymatic hydrolysis Uricularia polytricha
Protein ingredient can decompose；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.15MPa in fermentation pressure, fermentation temperature is 40 DEG C
Under the conditions of ferment 20h, obtain fermentation liquid；Yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion is broken
It splits, is conducive to the release of Polysaccharide from Auricularia Polytricha Sacc, be conducive to sufficiently extract the polysaccharide component in Uricularia polytricha；The enzymolysis liquid and yeast
Mass ratio is 100:1.4；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 6.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 4h, recycles ethyl alcohol,
Then revolving speed be 3000rpm under conditions of be centrifuged 10min, by obtained sediment temperature be 2 DEG C under conditions of vacuum decompression
Dry 2h, obtains Polysaccharide from Auricularia Polytricha Sacc；The volume ratio of the concentrate and ethanol solution is 1:4；The concentrate is the supernatant
The 1/4 of liquid product.
The yield for the Polysaccharide from Auricularia Polytricha Sacc that the present embodiment extracts is 3.46%.
Embodiment 2
The extracting method of the Polysaccharide from Auricularia Polytricha Sacc of the present embodiment, the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being 1.4:1:1.9；The enzyme activity amount of the complex enzyme cellulase, neutral proteinase and pectase is respectively 20000U/
G, 30000U/g and 30000U/g；The mass ratio of the Uricularia polytricha, water and complex enzyme is 1:35:0.1；
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under the conditions of at a temperature of 90 °C, obtains enzymolysis liquid；Pass through insoluble fibre, colloid and the plant egg in enzymatic hydrolysis Uricularia polytricha
Bai Chengfen can be decomposed；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.2MPa, the item that fermentation temperature is 40 DEG C in fermentation pressure
Ferment 20h under part, obtains fermentation liquid；Yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion rupture,
The release for being conducive to Polysaccharide from Auricularia Polytricha Sacc is conducive to sufficiently extract the polysaccharide component in Uricularia polytricha；The matter of the enzymolysis liquid and yeast
Amount is than being 100:1.4；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 8.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 2h, recycles ethyl alcohol,
Then revolving speed be 3000rpm under conditions of be centrifuged 10min, by obtained sediment temperature be 1 DEG C under conditions of vacuum decompression
Dry 4h, obtains Polysaccharide from Auricularia Polytricha Sacc；The volume ratio of the concentrate and ethanol solution is 1:4；The concentrate is the supernatant
The 1/4 of liquid product.
The yield for the Polysaccharide from Auricularia Polytricha Sacc that the present embodiment extracts is 3.43%.
Embodiment 3
The extracting method of the Polysaccharide from Auricularia Polytricha Sacc of the present embodiment, the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being 1.6:1:2.1；The enzyme activity amount of the complex enzyme cellulase, neutral proteinase and pectase is respectively 20000U/
G, 30000U/g and 30000U/g；The mass ratio of the Uricularia polytricha, water and complex enzyme is 1:35:0.1；
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under the conditions of at a temperature of 90 °C, obtains enzymolysis liquid；Pass through insoluble fibre, colloid and the plant egg in enzymatic hydrolysis Uricularia polytricha
Bai Chengfen can be decomposed；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.15MPa in fermentation pressure, fermentation temperature is 40 DEG C
Under the conditions of ferment 20h, obtain fermentation liquid；Yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion is broken
It splits, is conducive to the release of Polysaccharide from Auricularia Polytricha Sacc, be conducive to sufficiently extract the polysaccharide component in Uricularia polytricha；The enzymolysis liquid and yeast
Mass ratio is 100:1.4；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 8.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 3h, recycles ethyl alcohol,
Then revolving speed be 3000rpm under conditions of be centrifuged 10min, by obtained sediment temperature be 1 DEG C under conditions of vacuum decompression
Dry 4h, obtains Polysaccharide from Auricularia Polytricha Sacc；The volume ratio of the concentrate and ethanol solution is 1:4；The concentrate is the supernatant
The 1/4 of liquid product.
The yield for the Polysaccharide from Auricularia Polytricha Sacc that the present embodiment extracts is 3.42%.
Embodiment 4
The extracting method of the Polysaccharide from Auricularia Polytricha Sacc of the present embodiment, the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being 1.4:1:2.1；The enzyme activity amount of the complex enzyme cellulase, neutral proteinase and pectase is respectively 20000U/
G, 30000U/g and 30000U/g；The mass ratio of the Uricularia polytricha, water and complex enzyme is 1:35:0.1；
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under conditions of temperature is 100 DEG C, obtains enzymolysis liquid；Pass through insoluble fibre, colloid and the plant in enzymatic hydrolysis Uricularia polytricha
Protein ingredient can decompose；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.2MPa, the item that fermentation temperature is 40 DEG C in fermentation pressure
Ferment 20h under part, obtains fermentation liquid；Yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion rupture,
The release for being conducive to Polysaccharide from Auricularia Polytricha Sacc is conducive to sufficiently extract the polysaccharide component in Uricularia polytricha；The matter of the enzymolysis liquid and yeast
Amount is than being 100:1.4；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 8.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 2h, recycles ethyl alcohol,
Then revolving speed is centrifuged 10min under conditions of being 3000rpm, and by obtained sediment, vacuum subtracts under conditions of temperature is 1.5 DEG C
Dry 3h is pressed dry, Polysaccharide from Auricularia Polytricha Sacc is obtained；The volume ratio of the concentrate and ethanol solution is 1:4；The concentrate is on described
The 1/4 of supernatant volume.
The yield for the Polysaccharide from Auricularia Polytricha Sacc that the present embodiment extracts is 3.42%.
Embodiment 5
The extracting method of the Polysaccharide from Auricularia Polytricha Sacc of the present embodiment, the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is fibre
Tie up the mixture of plain enzyme, neutral proteinase and pectase；The matter of the complex enzyme cellulase, neutral proteinase and pectase
Amount is than being 1.5:1:2；The enzyme activity amount of the complex enzyme cellulase, neutral proteinase and pectase be respectively 20000U/g,
30000U/g and 30000U/g；The mass ratio of the Uricularia polytricha, water and complex enzyme is 1:35:0.1；
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then to 6.0
Enzyme deactivation under the conditions of at a temperature of 90 °C, obtains enzymolysis liquid；Pass through insoluble fibre, colloid and the plant egg in enzymatic hydrolysis Uricularia polytricha
Bai Chengfen can be decomposed；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.15MPa in fermentation pressure, fermentation temperature is 40 DEG C
Under the conditions of ferment 20h, obtain fermentation liquid；Yeast fermentation can make that the Uricularia polytricha tissue in enzymolysis liquid is fluffy, cell wall expansion is broken
It splits, is conducive to the release of Polysaccharide from Auricularia Polytricha Sacc, be conducive to sufficiently extract the polysaccharide component in Uricularia polytricha；The enzymolysis liquid and yeast
Mass ratio is 100:1.4；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will
Extracting solution is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by concentrate
PH value is adjusted to 8.0, and the ethanol solution that mass fraction is 95% then is added to the concentrate, stands alcohol and analyses 2h, recycles ethyl alcohol,
Then revolving speed be 3000rpm under conditions of be centrifuged 10min, by obtained sediment temperature be 2 DEG C under conditions of vacuum decompression
Dry 2h, obtains Polysaccharide from Auricularia Polytricha Sacc；The volume ratio of the concentrate and ethanol solution is 1:4；The concentrate is the supernatant
The 1/4 of liquid product.
The yield for the Polysaccharide from Auricularia Polytricha Sacc that the present embodiment extracts is 3.41%.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way.It is all according to invention skill
Art any simple modification, change and equivalence change substantially to the above embodiments, still fall within technical solution of the present invention
Protection scope in.
Claims (7)
1. a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc, which is characterized in that the extracting method are as follows:
S1, Uricularia polytricha is added to the water, complex enzyme is then added, be uniformly mixing to obtain mixture；The complex enzyme is cellulose
The mixture of enzyme, neutral proteinase and pectase；The mass ratio of the complex enzyme cellulase, neutral proteinase and pectase
For (1.4~1.6): 1:(1.9~2.1).
S2, the pH value for adjusting mixture obtained in S1 digest 80min under conditions of temperature is 60 DEG C, then in temperature to 6.0
Degree is enzyme deactivation under conditions of 90 DEG C~100 DEG C, obtains enzymolysis liquid；
S3, yeast is added into enzymolysis liquid obtained in S2, is 0.15MPa~0.2MPa, fermentation temperature 40 in fermentation pressure
Ferment 20h under conditions of DEG C, obtains fermentation liquid；
S4, by fermentation liquid obtained in S3, reflux extracts 120min under conditions of temperature is 60 DEG C, obtains extracting solution, will extract
Liquid is centrifuged 10min under conditions of revolving speed is 3000rpm, takes supernatant to be concentrated, obtains concentrate, by the pH value of concentrate
6.0~8.0 are adjusted to, the ethanol solution that mass fraction is 95% then is added to the concentrate, alcohol is stood and analyses 2h~4h, recycling
Ethyl alcohol, then revolving speed is centrifuged 10min under conditions of being 3000rpm, the condition for being 1 DEG C~2 DEG C in temperature by obtained sediment
Dry 2h~the 4h of lower vacuum decompression, obtains Polysaccharide from Auricularia Polytricha Sacc.
2. a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc according to claim 1, which is characterized in that in complex enzyme described in S1
The mass ratio of cellulase, neutral proteinase and pectase is 1.5:1:2.
3. according to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc described in claim 1, which is characterized in that fine in complex enzyme described in S1
The enzyme activity amount for tieing up plain enzyme, neutral proteinase and pectase is respectively 20000U/g, 30000U/g and 30000U/g.
4. according to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc described in claim 1, which is characterized in that Uricularia polytricha described in S1, water
Mass ratio with complex enzyme is 1:35:0.1.
5. according to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc described in claim 1, which is characterized in that enzymolysis liquid described in S3 and ferment
Female mass ratio is 100:1.4.
6. according to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc described in claim 1, which is characterized in that concentrate described in S4 and second
The volume ratio of alcoholic solution is 1:4.
7. according to a kind of extracting method of Polysaccharide from Auricularia Polytricha Sacc described in claim 1, which is characterized in that concentrate described in S4 is institute
State the 1/4 of supernatant volume.
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Cited By (1)
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| CN110642959A (en) * | 2019-10-17 | 2020-01-03 | 吉林农业大学 | Auricularia fuscosuccinea acidic polysaccharide, and preparation method and application thereof |
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| CN110642959A (en) * | 2019-10-17 | 2020-01-03 | 吉林农业大学 | Auricularia fuscosuccinea acidic polysaccharide, and preparation method and application thereof |
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