CN110478364A - The purposes of marine algae extract with reducing blood lipid effectiveness - Google Patents
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Abstract
The invention discloses the purposes of the marine algae extract with reducing blood lipid effectiveness, the purposes of the marine algae extract is to be used to prepare reducing blood lipid effervescent tablet.The present invention also provides a kind of oral administration liquor for reducing blood fat, it is active constituent that it, which is by a effective amount of algal polysaccharides, acceptable auxiliary material is added or complementary ingredient is prepared, the ingredient and its parts by weight of oral solution are as follows: 12-18 parts of algal polysaccharides, 18-23 parts of xylitol, 0.5-0.8 parts of Sucralose, 8-12 parts of fruit powder, 6-8 parts of PEG6000,40-50 parts of disintegrating agent, 0.02-0.04 parts of povidone, 0.3-0.8 parts of sweetener.Have the beneficial effect that the purposes that seaweed of the present invention is extracted is to be used to prepare reducing blood lipid effervescent tablet, the marine algae extract sulfate content and molecular weight are reasonable, purity is high, so that hypolipidemic activity is stronger, the Effervescent tablet disintegration time obtained is short, hydroscopicity is low.
Description
Technical field
The present invention relates to polysaccharide technical fields, more particularly, to the purposes of the marine algae extract with reducing blood lipid effectiveness.
Background technique
Seaweed (Algae) be the important component of living marine resources, for be grown in ocean containing chlorophyll or containing
The low cryptogam of other accessory pigments is that photosynthesis, the biology for manufacturing oxygen and ocean can be uniquely carried out in ocean
The basic producer of food chain, the balance and stability to marine ecology, there is imprint power.Marine algae is that have
The important sources of the bioactive substance of important biomolecule pharmaceutical value and structure diversity.In addition to industrial use, in recent years, seaweed
Derived polysaccharide has been considered as a kind of important bioactive natural products, they are reported with immunological regulation, anticoagulation, resist
The various actives such as tumour, antiviral, anti-mutation, hypoglycemic, reducing blood lipid, anti-inflammatory.As the extensive use and people of seaweed are to sea
Going deep into for this kind of important living matter understanding of polysaccharides, is increasingly taken seriously to the research of algal polysaccharides bioactivity, from
And becomes this subject in current life science and study one of most active field.Polysaccharide be constitute organism it is a kind of very
Important organic compound is the material base of life.
Marine algae provides source abundant for sulfated polysaccharides, deep to the sulfated polysaccharides with multiple biological activities
Very considerable commercial value will also be created by entering research.Polysaccharide compound maintains life in regulating cell division and cell growth
The many aspects such as life eubolism play a significant role, therefore research related with polysaccharide is increasingly valued by people.Sea
Algae quantity and kind in sea-plant is most, and polysaccharide accounts for about the 20%~70% of seaweed dry weight, is between alginic cell and thin
The general name of contained various polymeric carbohydrates intracellular, can be divided into three classes: the first kind is positioned at the outermost born of the same parents of cell
Wall polysaccharide is mostly crystallized in microfibre shape；Second class is the mucopolysaccharide between cell wall polysaccharides, mostly unsetting glue；Third
Class is to be present in intracellular storage polysaccharides.It is existing multiple studies have shown that, algal polysaccharides have multiple biological activities and medicinal valence
Value, such as antiviral, immunological regulation, antimicrobial antiphlogistic, alleviate radiotherapy side effect, antithrombotic at anti-oxidant, antitumor, reducing blood sugar and blood lipid
And the multiple efficacies such as anti-aging.Wherein, laminarin, laver amylose, sea grass polysaccharide, U. pertusa polysaccharide, Hijiki polysaccharide etc.
With significant effect for reducing blood fat.
Summary of the invention
The purpose of the present invention is to provide the purposes of the marine algae extract with reducing blood lipid effectiveness, which is to be used to prepare
Reducing blood lipid effervescent tablet, the marine algae extract sulfate content and molecular weight are reasonable, purity is high, so that hypolipidemic activity is stronger, system
The Effervescent tablet disintegration time obtained is short, hydroscopicity is low.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken are as follows:
The purposes of marine algae extract with reducing blood lipid effectiveness, the purposes of the marine algae extract are to be used to prepare reducing blood lipid effervesce
Piece.
Preferably, marine algae extract is algal polysaccharides, the molecular weight of algal polysaccharides is 10.28-15.55kDa, the seaweed
It is mainly fucoidan in polysaccharide, wherein sulfate content is 33.45%, in the sulfate content and molecular weight ranges
Fucoidan hypolipidemic activity it is stronger, the algal polysaccharides are by cooperateing with intracorporal antioxidase to enhance body antioxygen
Change ability removes free radical, restores intracorporal oxidation-reduction system balance, level of lipid is reduced, to protect body
Interior lipid-metabolism enzyme restores lipid and is normally metabolized；Promote lipoprotein metabolism key enzyme lecithin cholesterol acyltransferase
It is increased with hepatic lipase activity, accelerates TC and LDL catabolism；By inhibiting cholesterol biosynthesis rate-limiting enzyme HMG-CoA reductase, make
Internal cholesterol biosynthesis is reduced.
Further, the extracting method of algal polysaccharides, specific steps are as follows:
Step 1: being 1:26-33g/mL into seawood meal plus water by solid-liquid ratio, Extracting temperature is 75-85 DEG C, ultrasonic power is
The ultrasonic wave wave of 300-400W extracts 40-60min, and after extraction, 15-25rnin, supernatant are centrifuged at 3000-5000rpm
Liquid is concentrated into the 1/4 to 1/2 of original volume, and the dehydrated alcohol that the 3-5 times of volume of the concentrated liquid is added carries out precipitate polysaccharides, stands overnight,
Then be centrifuged 15-25rnin at 3000-5000rpm, pellet frozen it is dry seaweed Thick many candies, it is spare；
Step 2: being that 1:6-10 adds water into seaweed Thick many candies by solid-liquid ratio, be uniformly mixed, be 300-360U/g by enzyme additive amount
Papain and alkali protease, enzyme under conditions of temperature is 50-60 DEG C, pH is 7.2-8.2 is added with 200-280U/g
Solve 2-3h, after the completion of enzymatic hydrolysis at 90-100 DEG C enzyme deactivation 20-30min, be cooled to room temperature, be then centrifuged at 4000-6000rpm
14-18rnin, then Sevage reagent will be added with volume ratio 1:3-5 in supernatant, 20-30min is acutely shaken after mixing, is stood
Layering divides sub-cloud denatured protein and intersection organic solvent, 2-3 times repeatedly, obtains de- proteoglycan liquid, be concentrated into substance
Long-pending 1/4 to 1/2 adds the dehydrated alcohol of the 3-5 times of volume of the concentrated liquid, by the way of stirring while adding, until precipitating
Until being precipitated completely, precipitate polysaccharides are carried out, stands overnight, 14-18rnin, pellet frozen is then centrifuged at 4000-6000rpm
It is dry that the first polysaccharide of seaweed, the step take enzyme process and Sevage method to combine the protein in removal Thick many candies, enzymatic isolation method energy
It is enough to hydrolyze the protein and part glycoprotein that dissociate in liquid glucose, to greatly reduce the protein content in polysaccharide, still
Enzyme concentration is not suitable for being easily introduced external protein, therefore handles in enzymatic hydrolysis and then with Sevage method, can effectively remove
The protein for dissociating in polysaccharide liquid and digesting, and guarantee the presence of polysaccharide, effectively reduce the loss late of polysaccharide, while the party
Method can survivable polysaccharide component, guarantee the activity of polysaccharide；
Step 3: polysaccharide at the beginning of seaweed being dissolved in 0.04-0.06M sulfuric acid solution, the solution of 0.8-1.2% is made, then in 50-
3-5h is reacted at 70 DEG C, is terminated and is reacted with 2M NaOH, dialysis is lyophilized up to algal polysaccharides, and above-mentioned sulfuric acid solution contains 0.2-
0.3mM chinic acid, the weight ratio of D- chinic acid and L- chinic acid is 100:0.32-0.38 in chinic acid, and the step reaction is mild,
Sulfuric acid can be broken the glycosidic bond in fucoidan, and fucosan is cut into low molecular segment, available
Component is single, it is active preferably, the moderate fucoidan of molecular weight, but the sulfate in fucoidan simultaneously
Can fall off, and chinic acid can make the reaction rate for increasing the step, while can avoid excessive cutting of the sulfuric acid to glycosidic bond, mention
The yield of high target algal polysaccharides, while the addition of L- chinic acid can reduce the degree that falls off of sulfate, improve containing for sulfate
Amount, and then improve the lipid-lowering effect of algal polysaccharides.
Further, step 2 vanillic aldehyde containing 0.2-0.3% in Sevage reagent, the addition of vanillic aldehyde can make with
Polysaccharide is firmly combined or is come out by the protein quick release that polysaccharide wraps up, and then is denaturalized under the action of Sevage reagent, makes
Obtaining the proteinoid can be removed, and improve the purity of algal polysaccharides.
The present invention also provides a kind of reducing blood lipid effervescent tablets, and it is active constituent that it, which is by a effective amount of algal polysaccharides, are added
Acceptable auxiliary material or complementary ingredient are prepared, the ingredient and its parts by weight of effervescent tablet are as follows: 12-18 parts of algal polysaccharides, wood
18-23 parts of sugar alcohol, 0.5-0.8 parts of Sucralose, 8-12 parts of fruit powder, 6-8 parts of PEG6000,40-50 parts of disintegrating agent, povidone
0.02-0.04 parts, 0.3-0.8 parts of sweetener.Above-mentioned disintegrating agent is that weight ratio is that 1:1.5-1.7 sodium tartrate and sodium bicarbonate are mixed
Object is closed, in the proportional region, effervescent tablet has the advantage that disintegration time is short, hydroscopicity is low, and is disintegrated the pH value of rear solution
Change unobvious, has no adverse effects to mouthfeel, which can play the synergistic function of algal polysaccharides He other raw materials,
Enteral absorption enzyme can be directly acted on, makes to absorb enzymatic activity reduction, to reduce the absorption of TC, reduces blood lipids water
It is flat, while condensate can be combined into dietary fat in gastric acid, to inhibit the decomposition and metabolism of fat, and make without
The fat of digestion is expelled directly out in vitro, additionally it is possible to enhance the activity of lipoprotein lipase and hepatic lipase in serum, to reach drop
The purpose of low levels of lipid meets current blood fat reducing health products market, has preferable market prospects.
Compared with the prior art, the advantages of the present invention are as follows: 1) purposes of of the present invention marine algae extract be used to prepare drop
Blood lipid effervescent tablet, the marine algae extract enhance antioxidant ability of organism by the intracorporal antioxidase of collaboration or remove free radical,
Restore intracorporal oxidation-reduction system balance, reduce level of lipid, to protect the intracorporal lipid-metabolism enzyme of machine, restores
Lipid is normally metabolized；2) preparation method of marine algae extract of the invention is simple, can be made component it is single, it is active preferably, point
Son measures moderate algal polysaccharides, while can improve the content of sulfate, and then improves the lipid-lowering effect of algal polysaccharides；3) this hair
Bright effervescent tablet can play the synergistic function of algal polysaccharides He other raw materials, full to achieve the purpose that reduce blood lipid level
The current blood fat reducing health products market of foot, has preferable market prospects.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
The purposes of marine algae extract with reducing blood lipid effectiveness, the purposes of the marine algae extract are to be used to prepare reducing blood lipid effervesce
Piece.
Marine algae extract is algal polysaccharides, and the molecular weight of algal polysaccharides is 10.28-15.55kDa, master in the algal polysaccharides
It to be fucoidan, wherein sulfate content is 33.45%, the fucan in the sulfate content and molecular weight ranges
The hypolipidemic activity of sugar sulfate is stronger, the algal polysaccharides by cooperate with intracorporal antioxidase enhance antioxidant ability of organism or
Free radical is removed, restores intracorporal oxidation-reduction system balance, level of lipid is reduced, to protect the intracorporal lipid of machine
Metabolic enzyme restores lipid and is normally metabolized；Promote lipoprotein metabolism key enzyme lecithin cholesterol acyltransferase and hepatic lipase
Activity increases, and accelerates TC and LDL catabolism；By inhibiting cholesterol biosynthesis rate-limiting enzyme HMG-CoA reductase, keep internal gallbladder solid
Alcohol synthesis is reduced.
The extracting method of algal polysaccharides, specific steps are as follows:
Step 1: being 1:33g/mL into seawood meal plus water by solid-liquid ratio, Extracting temperature is 75 DEG C, ultrasonic power is 400W's
Ultrasonic wave wave extracts 40min, after extraction, is centrifuged 25rnin at 3,000 rpm, supernatant is concentrated into the 1/4 of original volume, adds
The dehydrated alcohol for entering 5 times of volume of the concentrated liquid carries out precipitate polysaccharides, stands overnight, is then centrifuged 25rnin at 3,000 rpm, precipitates
Seaweed Thick many candies are freeze-dried to obtain, it is spare；
Step 2: being that 1:6 adds water into seaweed Thick many candies by solid-liquid ratio, be uniformly mixed, be 360U/g and 200U/g by enzyme additive amount
Papain and alkali protease is added, digests 3h under conditions of temperature is 60 DEG C, pH is 7.2,90 after the completion of enzymatic hydrolysis
Enzyme deactivation 30min at DEG C, is cooled to room temperature, and is then centrifuged 25rnin at 3,000 rpm, then will add in supernatant with volume ratio 1:3
Entering Sevage reagent, 30min is acutely shaken after mixing, stratification divides sub-cloud denatured protein and intersection organic solvent,
2 times repeatedly, de- proteoglycan liquid is obtained, the 1/2 of original volume is concentrated into, adds the dehydrated alcohol of 3 times of volume of the concentrated liquid, is used
The mode stirred while adding until precipitation completely until precipitating, carries out precipitate polysaccharides, stands overnight, then at 3,000 rpm
It is centrifuged 25rnin, pellet frozen is dry that the first polysaccharide of seaweed, the step take enzyme process and Sevage method to combine removal Thick many candies
In protein, enzymatic isolation method can hydrolyze the protein to dissociate in liquid glucose and part glycoprotein, to greatly reduce polysaccharide
In protein content, but enzyme concentration is not suitable for being easily introduced external protein, therefore in enzymatic hydrolysis and then with Sevage method
Processing can effectively remove the protein for dissociating in polysaccharide liquid and digesting, and guarantee the presence of polysaccharide, effectively reduce more
Sugar loss late, while this method can survivable polysaccharide component, guarantee the activity of polysaccharide, it is above-mentioned to be contained in Sevage reagent
There is 0.2% vanillic aldehyde, the addition of vanillic aldehyde can be firmly combined with polysaccharide or be gone out by the protein quick release that polysaccharide wraps up
Come, and then be denaturalized under the action of Sevage reagent, which is removed, the purity of algal polysaccharides is improved；
Step 3: polysaccharide at the beginning of seaweed being dissolved in 0.06M sulfuric acid solution, 0.8% solution is made, is then reacted at 70 DEG C
3h is terminated with 2M NaOH and is reacted, dialysis, is lyophilized up to algal polysaccharides, above-mentioned sulfuric acid solution contains 0.3mM chinic acid, chinic acid
The weight ratio of middle D- chinic acid and L- chinic acid is 100:0.32, and the step reaction is mild, and sulfuric acid can be broken fucosan sulphur
Fucosan is cut into low molecular segment by the glycosidic bond in acid esters, available component is single, it is active preferably, molecule
Measure moderate fucoidan, but the sulfate in fucoidan can also fall off simultaneously, and chinic acid can make
Increase the reaction rate of the step, while can avoid excessive cutting of the sulfuric acid to glycosidic bond, improves the yield of target algal polysaccharides,
The addition of L- chinic acid can reduce the degree that falls off of sulfate simultaneously, improve the content of sulfate, and then improve algal polysaccharides
Lipid-lowering effect.
A kind of reducing blood lipid effervescent tablet, it be by a effective amount of algal polysaccharides be active constituent, be added acceptable auxiliary material or
Complementary ingredient is prepared, the ingredient and its parts by weight of effervescent tablet are as follows: 18 parts of algal polysaccharides, 18 parts of xylitol, Sucralose
0.8 part, 8 parts of fruit powder, 8 parts of PEG6000,40 parts of disintegrating agent, 0.04 part of povidone, 0.3 part of sweetener.Above-mentioned disintegrating agent is attached most importance to
Amount is than being 1:1.7 sodium tartrate and sodium bicarbonate mixture, in the proportional region, effervescent tablet short, moisture absorption with disintegration time
The low advantage of rate, and the pH value variation for being disintegrated rear solution is unobvious, has no adverse effects to mouthfeel, which can play sea
The synergistic function of polysaccharides and other raw materials can directly act on enteral absorption enzyme, make to absorb enzymatic activity reduction,
To reduce the absorption of TC, it is horizontal to reduce blood lipids, while condensate can be combined into dietary fat in gastric acid, thus
The decomposition and metabolism of fat are inhibited, and is expelled directly out undigested fat in vitro, additionally it is possible to enhance rouge in serum
The activity of albumen lipase and hepatic lipase meets current blood fat reducing health products market, has to achieve the purpose that reduce blood lipid level
Preferable market prospects.
Embodiment 2:
The purposes of marine algae extract with reducing blood lipid effectiveness, the purposes of the marine algae extract are to be used to prepare reducing blood lipid effervesce
Piece.
Marine algae extract is algal polysaccharides, and the molecular weight of algal polysaccharides is 10.28-15.55kDa, master in the algal polysaccharides
It to be fucoidan, wherein sulfate content is 33.45%.
The extracting method of algal polysaccharides, specific steps are as follows:
Step 1: being 1:26g/mL into seawood meal plus water by solid-liquid ratio, Extracting temperature is 75 DEG C, ultrasonic power is 400W's
Ultrasonic wave wave extracts 40min, after extraction, 15rnin is centrifuged at 5000rpm, supernatant is concentrated into the 1/2 of original volume, adds
The dehydrated alcohol for entering 3 times of volume of the concentrated liquid carries out precipitate polysaccharides, stands overnight, 15rnin is then centrifuged at 5000rpm, precipitates
Seaweed Thick many candies are freeze-dried to obtain, it is spare；
Step 2: being that 1:10 adds water into seaweed Thick many candies by solid-liquid ratio, be uniformly mixed, be 300U/g and 280U/ by enzyme additive amount
Papain and alkali protease is added in g, digests 2h under conditions of temperature is 50 DEG C, pH is 8.2, after the completion of enzymatic hydrolysis
Enzyme deactivation 20min, is cooled to room temperature at 100 DEG C, is then centrifuged 15rnin at 5000rpm, then by supernatant with volume ratio 1:5
Sevage reagent is added, 20min is acutely shaken after mixing, stratification divides sub-cloud denatured protein and intersection organic molten
Agent 2 times repeatedly, obtains de- proteoglycan liquid, is concentrated into the 1/2 of original volume, adds the dehydrated alcohol of 3 times of volume of the concentrated liquid,
By the way of stirring while adding, until precipitation completely until precipitating, precipitate polysaccharides is carried out, stands overnight, then exists
Be centrifuged 15rnin under 5000rpm, pellet frozen it is dry the first polysaccharide of seaweed, the above-mentioned chinese cymbidium in Sevage reagent containing 0.3%
Element；
Step 3: polysaccharide at the beginning of seaweed being dissolved in 0.04M sulfuric acid solution, 1.2% solution is made, is then reacted at 50 DEG C
5h is terminated with 2M NaOH and is reacted, dialysis, is lyophilized up to algal polysaccharides, above-mentioned sulfuric acid solution contains 0.2mM chinic acid, chinic acid
The weight ratio of middle D- chinic acid and L- chinic acid is 100:0.38.
A kind of reducing blood lipid effervescent tablet, it be by a effective amount of algal polysaccharides be active constituent, be added acceptable auxiliary material or
Complementary ingredient is prepared, the ingredient and its parts by weight of effervescent tablet are as follows: 12 parts of algal polysaccharides, 23 parts of xylitol, Sucralose
0.5 part, 12 parts of fruit powder, 6 parts of PEG6000,50 parts of disintegrating agent, 0.02 part of povidone, 0.8 part of sweetener.Above-mentioned disintegrating agent is attached most importance to
Amount is than being 1:1.5 sodium tartrate and sodium bicarbonate mixture.
Embodiment 3:
The purposes of marine algae extract with reducing blood lipid effectiveness, the purposes of the marine algae extract are to be used to prepare reducing blood lipid effervesce
Piece.
Marine algae extract is algal polysaccharides, and the molecular weight of algal polysaccharides is 10.28-15.55kDa, master in the algal polysaccharides
It to be fucoidan, wherein sulfate content is 33.45%.
The extracting method of algal polysaccharides, specific steps are as follows:
Step 1: being 1:30g/mL into seawood meal plus water by solid-liquid ratio, Extracting temperature is 80 DEG C, ultrasonic power is 350W's
Ultrasonic wave wave extracts 50min, after extraction, 20rnin is centrifuged at 4000rpm, supernatant is concentrated into the 1/3 of original volume, adds
The dehydrated alcohol for entering 4 times of volume of the concentrated liquid carries out precipitate polysaccharides, stands overnight, 20rnin is then centrifuged at 4000rpm, precipitates
Seaweed Thick many candies are freeze-dried to obtain, it is spare；
Step 2: being that 1:8 adds water into seaweed Thick many candies by solid-liquid ratio, be uniformly mixed, be 330U/g and 250U/g by enzyme additive amount
Papain and alkali protease is added, digests 2.5h under conditions of temperature is 55 DEG C, pH is 7.5, after the completion of enzymatic hydrolysis
Enzyme deactivation 25min, is cooled to room temperature at 95 DEG C, is then centrifuged 20rnin at 4000rpm, then by supernatant with volume ratio 1:4
Sevage reagent is added, 25min is acutely shaken after mixing, stratification divides sub-cloud denatured protein and intersection organic molten
Agent 3 times repeatedly, obtains de- proteoglycan liquid, is concentrated into the 1/3 of original volume, adds the dehydrated alcohol of 4 times of volume of the concentrated liquid,
By the way of stirring while adding, until precipitation completely until precipitating, precipitate polysaccharides is carried out, stands overnight, then exists
Be centrifuged 20rnin under 4000rpm, pellet frozen it is dry the first polysaccharide of seaweed, the above-mentioned perfume (or spice) in Sevage reagent containing 0.25%
Lan Su；
Step 3: polysaccharide at the beginning of seaweed being dissolved in 0.05M sulfuric acid solution, 1.0% solution is made, is then reacted at 60 DEG C
4h is terminated with 2M NaOH and is reacted, dialysis, is lyophilized up to algal polysaccharides, above-mentioned sulfuric acid solution contains 0.25mM chinic acid, Kui Ni
The weight ratio of D- chinic acid and L- chinic acid is 100:0.35 in acid.
A kind of reducing blood lipid effervescent tablet, it be by a effective amount of algal polysaccharides be active constituent, be added acceptable auxiliary material or
Complementary ingredient is prepared, the ingredient and its parts by weight of effervescent tablet are as follows: 15 parts of algal polysaccharides, 20 parts of xylitol, Sucralose
0.6 part, 10 parts of fruit powder, 7 parts of PEG6000,45 parts of disintegrating agent, 0.03 part of povidone, 0.5 part of sweetener.Above-mentioned disintegrating agent is attached most importance to
Amount is than being 1:1.6 sodium tartrate and sodium bicarbonate mixture.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (8)
1. the purposes of the marine algae extract with reducing blood lipid effectiveness, it is characterised in that: the marine algae extract is used to prepare drop blood
Rouge effervescent tablet, the marine algae extract are algal polysaccharides, and the molecular weight of the algal polysaccharides is 10.28-15.55kDa.
2. the purposes of the marine algae extract according to claim 1 with reducing blood lipid effectiveness, it is characterised in that: the seaweed
It is mainly fucoidan in polysaccharide, wherein sulfate content is 33.45%.
3. the purposes of the marine algae extract according to claim 1 with reducing blood lipid effectiveness, it is characterised in that: the seaweed
The extracting method of polysaccharide the following steps are included:
Step 1: being 1:26-33g/mL into seawood meal plus water by solid-liquid ratio, Extracting temperature is 75-85 DEG C, ultrasonic power is
The ultrasonic wave wave of 300-400W extracts 40-60min, is centrifuged after extraction, and supernatant is concentrated into the 1/4 to 1/2 of original volume, then
Be added the 3-5 times of volume of the concentrated liquid dehydrated alcohol alcohol precipitation, centrifugation, pellet frozen it is dry seaweed Thick many candies, it is spare；
Step 2: being that 1:6-10 adds water into seaweed Thick many candies by solid-liquid ratio, be uniformly mixed, be 300-360U/g by enzyme additive amount
Papain and alkali protease, enzyme under conditions of temperature is 50-60 DEG C, pH is 7.2-8.2 is added with 200-280U/g
Solve 2-3h, after the completion of enzymatic hydrolysis at 90-100 DEG C enzyme deactivation 20-30min, be cooled to room temperature, be centrifuged, then by supernatant with volume
Sevage reagent is added than 1:3-5,20-30min is acutely shaken after mixing, stratification divides sub-cloud denatured protein and friendship
Organic solvent at boundary 2-3 times repeatedly, obtains de- proteoglycan liquid, is concentrated into the 20-30% of original volume, adds 3-5 times and be concentrated
Liquid product dehydrated alcohol alcohol precipitation, centrifugation, pellet frozen it is dry seaweed just polysaccharide；
Step 3: polysaccharide at the beginning of seaweed being dissolved in 0.04-0.06M sulfuric acid solution, the solution of 0.8-1.2% is made, then in 50-
3-5h is reacted at 70 DEG C, is terminated and is reacted with 2M NaOH, dialysis is lyophilized up to algal polysaccharides.
4. the preparation method of algal polysaccharides according to claim 3, it is characterised in that: the step 2 Sevage reagent
In the vanillic aldehyde containing 0.2-0.3%.
5. the preparation method of algal polysaccharides according to claim 3, it is characterised in that: sulfuric acid solution contains in the step 3
There is 0.2-0.3mM chinic acid.
6. the preparation method of algal polysaccharides according to claim 5, it is characterised in that: in the chinic acid D- chinic acid and
The weight ratio of L- chinic acid is 100:0.32-0.38.
7. a kind of reducing blood lipid effervescent tablet, it is active constituent that it, which is by a effective amount of algal polysaccharides, acceptable auxiliary material or auxiliary is added
Helping property ingredient is prepared, it is characterised in that: the ingredient and its parts by weight of the effervescent tablet are as follows: 12-18 parts of algal polysaccharides, xylose
18-23 parts of alcohol, 0.5-0.8 parts of Sucralose, 8-12 parts of fruit powder, 6-8 parts of PEG6000,40-50 parts of disintegrating agent, povidone
0.02-0.04 parts, 0.3-0.8 parts of sweetener.
8. a kind of reducing blood lipid effervescent tablet according to claim 7, it is characterised in that: it is 1 that the disintegrating agent, which is weight ratio:
1.5-1.7 sodium tartrate and sodium bicarbonate mixture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810192826.0A CN110478364A (en) | 2018-03-09 | 2018-03-09 | The purposes of marine algae extract with reducing blood lipid effectiveness |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114271423A (en) * | 2021-12-31 | 2022-04-05 | 温州大学 | Trehalose-fiber composite effervescent tablet and preparation method thereof |
| CN115505049A (en) * | 2022-09-27 | 2022-12-23 | 福建农林大学 | Porphyra haitanensis polysaccharide and porphyra haitanensis polysaccharide effervescent tablets with blood fat reducing effect and preparation method thereof |
-
2018
- 2018-03-09 CN CN201810192826.0A patent/CN110478364A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114271423A (en) * | 2021-12-31 | 2022-04-05 | 温州大学 | Trehalose-fiber composite effervescent tablet and preparation method thereof |
| CN114271423B (en) * | 2021-12-31 | 2023-09-26 | 温州大学 | Trehalose fiber composite effervescent tablet and preparation method thereof |
| CN115505049A (en) * | 2022-09-27 | 2022-12-23 | 福建农林大学 | Porphyra haitanensis polysaccharide and porphyra haitanensis polysaccharide effervescent tablets with blood fat reducing effect and preparation method thereof |
| CN115505049B (en) * | 2022-09-27 | 2023-12-08 | 福建农林大学 | Porphyra haitanensis polysaccharide and Porphyra haitanensis polysaccharide effervescent tablet with blood lipid reducing effect and preparation method thereof |
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