JPWO2008123417A1 - Anti-fatigue - Google Patents

Abstract

An anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer containing Tedokoro or a processed product derived from Tedokoro as an active ingredient, foods, feeds, pharmaceuticals containing these agents, and methods for producing them.

Description

  The present invention relates to an anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer useful in the medical and health food fields.

  In recent years, with the increase in health consciousness and the development of sports science, the development of food with endurance enhancing action, fatigue recovery or prevention action, nourishing tonic action and muscle strengthening action has been promoted. Such foods not only improve the ability of athletes, but also enhance the physical or mental endurance needed to have a normal social life and promote recovery and prevention of fatigue. Development is also desired by general consumers. Especially in developed countries, physical fatigue, mental fatigue, and overwork caused by stress and life rhythm disruption are problems, and foods that exhibit anti-fatigue, endurance, nourishment, or muscle enhancement Development of pharmaceutical materials is desired. For example, Patent Document 1 discloses an anti-fatigue agent containing coenzyme Q10 and α-lipoic acid, and Patent Document 2 discloses an endurance enhancer and anti-fatigue agent containing a gold wire lotus. Has been.

  There are many kinds of yams, and in Japan, varieties such as Daijo, Ginenjo, Nagaimo, Togekokoro are mainly cultivated and edible. Togedokoro is cultivated in the south of Kyushu and is said to have originated in Thailand and Vietnam.

JP 2006-137730 A JP 2005-200390 A

  The object of the present invention is to provide an anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer useful in the medical and health food fields, food containing the agent, feed or medicine, and food useful as the agent It is in providing the extract of this, and the manufacturing method of the said extract.

  The first invention of the present invention relates to an anti-fatigue agent, endurance enhancer, nourishing tonic, or muscle enhancer containing Togekokoro or Togedokoro-derived processed products as active ingredients. In the first invention of the present invention, examples of the processed product derived from Tedokoro include dry powder, water, ethanol, water-containing ethanol or acetone extract, and fractions obtained from the extract.

  The second invention of the present invention relates to a food or feed containing the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer of the first invention of the present invention.

  The third invention of the present invention relates to a medicament containing the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer of the first invention of the present invention.

  4th invention of this invention is related with the manufacturing method of an extract including the process of extracting Tedokoro with water, ethanol, hydrous ethanol, or acetone. In the fourth invention of the present invention, the water-containing ethanol is exemplified by 1-85% (v / v) water-containing ethanol.

  The fifth invention of the present invention relates to an extract obtainable by the method of the fourth invention of the present invention.

  6th invention of this invention is related with the foodstuff or feed containing the extract of 5th invention of this invention.

  7th invention of this invention is related with the pharmaceutical containing the extract of 5th invention of this invention.

  In addition, as another aspect of the present invention, there is provided a fatigue recovery or prevention method, endurance enhancement method, nutrition tonic method, or muscle enhancement method, which includes a step of administering Tedokoro or a processed product derived from Tedokoro. Here, the administration of Togedokoro or the processed product derived from Togedokoro can be administered as the agent of the first invention of the present invention, the food or feed of the second invention of the present invention, or the pharmaceutical of the third invention of the present invention.

  Moreover, as another aspect of the present invention, there is provided a spine or a spine-derived treated product for use in a method for recovery or prevention of fatigue, a method for enhancing endurance, a method for enhancing tonicity or a method for strengthening muscles. The togedokororo or togedokuro-derived processed product can be used as the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer, the food of the present invention, or the pharmaceutical of the present invention.

  Moreover, as another aspect of the present invention, there is provided the use of Togedokoro or Togedokoro-derived processed products in the manufacture of an anti-fatigue agent, endurance enhancing agent, nourishing tonic or muscle enhancing agent. In addition, the present invention provides the use of Tedokoro or a processed product derived from Tedokoro in the manufacture of a food for anti-fatigue, endurance enhancement, nutrition tonic or muscle enhancement. In addition, the present invention provides the use of Tedokoro or a processed product derived from Tedokoro in the manufacture of a medicament for anti-fatigue, endurance enhancement, nutrition tonic or muscle enhancement.

  In addition, as another aspect of the present invention, in the production, containing a process to indicate that it is used for fatigue recovery or prevention, endurance enhancement, nourishment tonic or muscle strengthening, containing a togedokororo or togedokuro derived treatment A method for producing a food product is provided.

  Moreover, as another aspect of the present invention, there is also provided a food or feed for anti-fatigue, for enhancing endurance, for enhancing nutrition, or for enhancing muscle, characterized by containing Tedokoro or a processed product derived from Tedokoro. Examples of the food or feed include food or feed with an indication that it is used for recovery or prevention of fatigue, endurance enhancement, nutrition tonic or muscle enhancement.

  According to the present invention, useful as an anti-fatigue agent, endurance enhancer, nourishing tonic, or muscle enhancer containing Tedokoro or Tedokoro-derived processed product as an active ingredient, food, feed or medicine containing the agent, and the agent An extract derived from the swordfish and a method for producing the extract are provided. The anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer is useful not only for improving the ability of athletes but also as a material for foods or medicines that have a fatigue recovery or preventive action in social life. is there.

  The togekokoro (scientific name Dioscorea esculenta) used in the present invention refers to a kind of yam cultivated mainly in the south of Kyushu, which is also called potato. Any portion may be used for the spine, and there is no particular limitation. For example, rhizome, root, stem, leaf, or a mixture thereof can be used, and particularly preferably, rhizome can be used. .

  In the present invention, there is no particular limitation on the processed product derived from Tedokoro, for example, there is no particular limitation as long as the raw material Tedokoro has undergone some processing, for example, dry powder, ground product, extract, pulverized product, It refers to squeezed juice, crushed material, chemically treated product, and enzyme-treated product, and particularly preferably a dry powder, ground product or extract.

  In the present invention, examples of the dry powder include a pulverized product derived from Tedokoro used in the present invention (hereinafter sometimes referred to as the pulverized product of the present invention). Examples of the method for producing the pulverized product of the present invention include a method of obtaining a powdered pulverized pulverized product by drying the rhizomes of the stalks, appropriately performing far-infrared treatment, and pulverizing them using a pulverizer. . Further, a pulverized product may be obtained by freeze pulverization or pulverization after freeze-drying.

  In the present invention, the ground product refers to a product obtained by grinding down a tuber of thorny roll, and is usually prepared by using various automatic grinders or by manually grinding it.

  In the present specification, an extract refers to a substance itself obtained through a process of performing an extraction operation on a raw material plant using an extraction solvent. The extraction can be performed as follows by a known extraction method. For example, after pulverizing or chopping the raw material, it can be performed batchwise or continuously using a solvent. The extraction solvent for obtaining the extract is not particularly limited, but water, ethanol, methanol, isopropyl alcohol and other alcohols, acetone, methyl ethyl ketone and other ketones, methyl acetate, ethyl acetate, chloroform and other hydrophilic or An oleophilic solvent can be used, and it can be used alone or as a mixture as appropriate. Examples of using the mixed solution as an extraction solvent are not particularly limited, but various aqueous solutions can be used, for example, 10 to 100% (v / v), preferably 20 to 90% (v / v), More preferably, various aqueous solutions of 40 to 80% (v / v) can be used. In the present invention, water, ethanol, water-containing ethanol or acetone is particularly preferably exemplified as the extraction solvent. As water-containing ethanol, 1 to 85% (v / v) water-containing ethanol is preferably used. The amount of the extraction solvent may be determined as appropriate. Usually, the amount of the raw material plant in the form of the raw material plant at the time of use (for example, a raw plant if the raw plant is a raw plant) is preferably 0. The extraction solvent may be used in an amount of 01 to 40 times, more preferably 1 to 40 times. The extraction temperature may be appropriately determined according to the purpose, but in the case of water extraction, it is usually preferably 4 to 130 ° C, more preferably 10 to 100 ° C. Moreover, when ethanol is contained in a solvent, the range of 4-60 degreeC is suitable from a safety viewpoint. The extraction time may be determined in consideration of the extraction efficiency. Usually, the raw material, the extraction solvent, and the extraction temperature are set so as to be preferably in the range of several seconds to several days, more preferably 5 minutes to 24 hours. Is preferred. The extraction operation may be performed, for example, while stirring or standing, and may be repeated several times as necessary. By the above operation, the extract used in the present invention can be obtained. If necessary, the extract is treated by filtration, centrifugation, concentration, ultrafiltration, molecular sieving, etc., and an extract exhibiting anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle enhancing action as described later is appropriately used. It can also be extracted. It is also possible to use two or more types of extracts obtained by different extraction methods from raw material plants.

  Hereinafter, as the extract of the present invention, a method for producing a hydrous ethanol extract will be exemplified. As the raw material, Tegedokoro uses the rhizome itself, its dried product, sliced dried product or dry powder, and the raw material is, for example, 1 to 50 times the dry powder weight, preferably 2 to 40 times, more preferably Extract by immersing in 5 to 20 times the amount of water-containing ethanol. The hydrous ethanol is 1 to 85% (v / v), preferably 5 to 80% (v / v), more preferably 10 to 75% (v / v). The extraction conditions are 4 to 130 ° C., preferably 4 to 110 ° C., more preferably 4 to 100 ° C., particularly preferably 4 to 60 ° C., 5 minutes to 48 hours, preferably 30 minutes to 36 hours, and more preferably. 1 to 24 hours, and the extraction operation is carried out by standing, stirring or shaking. The obtained extract is separated into an extract and a residue by filtration or centrifugation, and the extract can be used as the hydrous ethanol extract of the present invention. The water-containing ethanol extract of the present invention exhibits higher anti-fatigue action, endurance enhancing action, nourishing tonic action, and muscle enhancing action as compared with dried powder of Togekokoro.

  In the present invention, the extract of the present invention also includes a fraction obtained by fractionating the extract of the present invention by a known method and a fraction obtained by repeating the fractionation operation a plurality of times. Is done. Examples of the fractionation means include extraction, fractional precipitation, column chromatography, thin layer chromatography and the like. Here, as the extraction, the above extraction solvent can be used as appropriate.

  Moreover, the squeezed liquor derived from Tedokoro used for this invention can also be used as a processed material of this invention. The method for producing the juice is not particularly limited as long as it is a known plant juice extraction method. For example, the juice can be extracted using a screw type, gear type, cutter type or other squeezer or juicer. Further, as a pretreatment, the juice can be obtained by chopping or grinding and squeezing with the above-mentioned juicer or cloth.

  The crushed material is a material plant that has been crushed and generally has a larger tissue piece than the pulverized material, and can be produced, for example, by using a crusher. The chemical treatment product is not particularly limited, but refers to a product obtained by subjecting the raw material plant to acid treatment, alkali treatment, oxidation treatment, reduction treatment, etc., for example, hydrochloric acid, sulfuric acid, nitric acid, citric acid, It can be produced by immersing the raw material plant in an aqueous solution containing an inorganic or organic acid such as acetic acid, or an inorganic or organic base such as sodium hydroxide, potassium hydroxide or ammonia. Chemically treated products include all those derived from plants that have undergone chemical treatment as described above. The enzyme-treated product refers to, for example, an enzyme-treated product such as pectinase, cellulase, xylanase, amylase, mannanase, glucosidase, etc., and an enzyme reaction product (for example, fermented product) using a microorganism as an enzyme source. The enzyme can be produced by acting in an appropriate buffer. The enzyme-treated product includes everything derived from a plant body that has been subjected to the enzyme treatment as described above.

  In the present specification, the anti-fatigue agent, endurance enhancer, nourishing tonic, and muscle enhancer are used alone or in combination with other components such as excipients, using Togedokoro or Togedokoro-derived processed products as active ingredients Used. In the present invention, the shape of the agent is not particularly limited, but may be any of powder, solid, and liquid. Further, the processed product can be granulated by a known method and used as a granular solid. The granulation method is not particularly limited, but rolling granulation, stirring granulation, fluidized bed granulation, air flow granulation, extrusion granulation, compression molding granulation, pulverization granulation, spray granulation, spray granulation or spray granulation Examples are grains. Further, the powdery processed product can be dissolved in a liquid, for example, water or alcohol, to form a liquid and used as the agent.

  From the results of the swimming test described in Examples 2, 4, 6, 8, 9, 13, and 20 described later, the present inventors made anti-fatigue action, endurance enhancement action, and nourishing tonic by the inventors. It was found for the first time to have an effect. That is, according to the present invention, an anti-fatigue agent, endurance enhancer, and nourishing tonic are provided that contain togekokoro or a treated product derived from togedokoro. Such anti-fatigue action, endurance enhancing action or nourishing tonic action, as described in Control Example 2 below, was not observed in the same genus Ganoderma as Tedokoro, but was peculiar to Tedokoro. . Moreover, in Example 7 described later, it was found that Togekokoro or Togedokoro-derived processed products have a blood creatine kinase increase inhibitory action. Blood creatine kinase is known as a marker of fatigue that rises with prolonged exercise or exercise with strong muscle contraction. Furthermore, in Examples 14 and 15 to be described later, it has been found for the first time that Tedokoro or a processed product derived from Tedokoro has an inhibitory effect on the increase in blood lactic acid level. That is, from these results, it was also biochemically shown that the togedokororo or togedokuro derived product has an endurance enhancing action. Furthermore, as described in Example 21 described later, the present inventors have found that the present invention has a muscle-enhancing effect in the spine or the spine-derived treatment product. That is, according to the present invention, there is provided a muscle strengthening agent that contains a spider or a spider-derived processed product. In addition, as another aspect of the present invention, there are also provided a blood creatine kinase elevation inhibitor and a blood lactic acid level elevation inhibitor containing Tedokoro or a processed product derived from Tedokoro as an active ingredient.

  In this specification, fatigue refers to a phenomenon in which the function of muscles, nerves, and the like deteriorates due to overuse, where the decrease in function refers to quantitative or qualitative physical and mental work ability. Means a significant decline. That is, although there is no particular limitation, it means physical fatigue after exercise such as swimming, mental fatigue after intellectual labor, and physical and mental combined fatigue accumulated in living social life. Furthermore, fatigue in the present specification includes conditions that lead to chronic fatigue syndrome and death from overwork. The anti-fatigue agent means a composition that recovers and reduces such fatigue states and further prevents fatigue.

  In the specification of the present application, endurance means physical strength that can continue to exercise for a long time, and the endurance enhancer means a composition that can be prolonged by taking it.

  In the present specification, nourishing tonic is a physical or mental ability that is further improved from a healthy state, such as an action to improve a healthy state, or an increase in energy, by providing nutrition during physical or mental fatigue. It means a strengthening action, and a nourishing tonic means a composition that exhibits such action.

  The present invention also provides a food or feed containing the anti-fatigue agent, endurance enhancer, nourishing tonic and muscle enhancer (hereinafter sometimes referred to as the food or feed of the present invention). The food or feed of the present invention is extremely useful as an anti-fatigue, endurance enhancing, nourishing tonic or muscle enhancing food or feed due to its anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle strengthening agent. It is. In particular, it is extremely useful for functional foods (general foods for maintaining health, nutritional functional foods) with the expectation of the above action. Further, since the food of the present invention exhibits the anti-fatigue action, endurance enhancement action, nourishing tonic action or muscle enhancement action, the chronic fatigue, those who are tired or tend to accumulate, endurance and muscle It is particularly useful as a food for specified health use suitable for those who want to enhance it.

  In the present specification, the “containing” includes the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer (hereinafter referred to as the agent of the present invention) in food, feed or medicine. The above-mentioned “addition” refers to an embodiment in which the agent of the present invention is added to a raw material for food, and the “dilution” refers to an embodiment in which a raw material for food is added to the agent of the present invention. It is.

  There is no limitation in particular in the manufacturing method of the foodstuff of this invention. For example, blending, cooking, processing, and the like may be in accordance with those of general foods and can be produced by their production method, and the obtained food may contain or be blended with the agent of the present invention. .

  The food of the present invention is not particularly limited. For example, a processed grain product (wheat flour processed product, processed starch product, premix processed product, noodles, macaroni, Breads, bean paste, buckwheat, rice cakes, rice noodles, harsame, packaging rice cakes), processed oils and fats (plastic oils, tempura oil, salad oil, mayonnaises, dressings, etc.), processed soybean products (tofu, miso, natto, etc.) ), Processed meat products (ham, bacon, press ham, sausage, etc.), marine products (frozen groundnut, kamaboko, chikuwa, hanpen, fried fish, tsumire, streaks, fish ham, sausage, bonito, processed fish eggs, canned fish , Tsukuda boiled etc.), dairy products (raw milk, cream, yogurt, butter, cheese, condensed milk, powdered milk, ice cream, etc.), processed vegetables and fruits (pace) , Jams, pickles, fruit drinks, vegetable drinks, mixed drinks, etc.), confectionery (jelly, gum, candy, chocolate, cookies, biscuits, confectionery breads, cakes, candy sweets, rice confectionery, etc.), alcoholic drinks (Sake, Chinese liquor, wine, whiskey, shochu, vodka, brandy, gin, rum, beer, soft alcoholic beverage, fruit liquor, liqueur, etc.), beverages (green tea, tea, oolong tea, coffee, soft drink, lactic acid beverage) Etc.), seasonings (soy sauce, sauce, vinegar, mirin, etc.), canned / bottled / bagged foods (beef rice, kettle rice, red rice, curry, other cooked foods), semi-dried or concentrated foods (lever paste, Other spreads, buckwheat noodle soup, concentrated soup), dried food (instant noodles, instant curry, instant coffee, powdered dice) Sauce, powdered soup, instant miso soup, cooked food, cooked drink, cooked soup, etc.), frozen food (sukiyaki, chawanmushi, eel cabbage, hamburg steak, shumai, dumplings, various sticks, fruit cocktails, etc.), solid food , Liquid foods (soups, etc.), processed agricultural products such as spices, livestock processed products, processed fishery products, etc. Particularly preferred are sports foods and drinks.

  Moreover, the shape of the food of the present invention is not particularly limited as long as the agent of the present invention, for example, a dry powder of swordfish or a water-containing ethanol extract is contained singly or plurally, added and / or diluted. ), Granule, capsule, jelly, and other shapes that are easy to ingest orally. For example, when producing a tablet-like food, there is no particular limitation, but as described in Examples 10 and 11 below, suitable excipients such as dextrin, reduced maltose starch syrup, crystalline cellulose, fine silicon dioxide, It can be produced by mixing with glycerin fatty acid ester, lactose, maltose, sugar, starch, trehalose, sucrose fatty acid ester, other various dietary fibers, various sugars, and the like, and tableting with a tableting machine. In the case of producing a granular food, there is no particular limitation, but as described in Example 16 below, it is mixed with the appropriate excipient, other various dietary fibers, various sugars, etc. It can be manufactured by a granulator. In the case of producing a capsule-shaped food, as described in Examples 17 and 18 below, it is mixed with the appropriate excipient, various dietary fibers, various saccharide emulsifiers, etc. Agent can be produced.

  Further, as a beverage, for example, as described in Example 19 below, the above-mentioned extract from Tedokoro, for example, water, water-containing ethanol or ethanol extract, various emulsifiers such as polyglycerin fatty acid ester, sucrose fatty acid ester, mono fatty acid Ester, organic acid fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, stearoyl calcium lactate, soybean lecithin, egg yolk lecithin and the like are mixed with an emulsifier and dissolved in water to produce a beverage.

  There is no particular limitation on the content of the above-mentioned squirrel or processed product derived from the stalk in the food of the present invention, and can be appropriately selected from the viewpoint of its functionality and activity expression. The intake may be preferably 1 to 2000 mg / kg body weight, more preferably 5 to 1000 mg / kg body weight, and still more preferably 10 to 500 mg / kg body weight.

  The food or feed of the present invention may be other ingredients that can be used in the same application as the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer of the present invention, such as garlic, garlic extract, egg yolk, and sardine. It can be blended with extracts, propolis, royal jelly, ginseng, taurine, cordyceps, indian oyster, viper, coenzyme Q10, α-lipoic acid, oyster mushroom, placenta extract, hydroxypropylated starch, various amino acids and the like.

  In addition, according to the present invention, in the production of a food containing a toggedokoro or a processed product derived from a toggedokoro, including a step of displaying that it is used for recovery from fatigue or prevention, endurance enhancement, nourishing tonic or muscle strengthening A method is also provided. Here, “to be used for fatigue recovery or prevention, endurance enhancement, nutrition tonic or muscle enhancement” means “to recover from fatigue or prevention, endurance for food itself, containers, packaging and / or pamphlets” "Indicates that it will be used for enhancement, nourishment or muscle enhancement".

  In addition, the present invention provides a biological feed containing an agent of the present invention, that is, containing, adding and / or diluting, and exhibiting anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle enhancing action. Furthermore, as another aspect, there is also provided a method for breeding an organism characterized by administering the agent of the present invention to the organism. Moreover, as another aspect of the present invention, there is provided a biological breeding agent comprising the agent of the present invention.

  In the present specification, the organism is not limited, and examples thereof include farm animals, pet animals, and competition animals. Aquaculture animals include horses, cattle, pigs, sheep, goats, camels, llamas and other domestic animals, mice, rats, guinea pigs, rabbits, and other laboratory animals, chickens, ducks, turkeys, eagle birds and other poultry, fish, crustaceans or shellfish Is exemplified. Examples of pet animals include dogs and cats. A racehorse etc. are illustrated as a competition animal. Examples of animal feed include feed for maintaining and / or improving physical condition. Examples of biological breeding agents include soaking agents, feed additives, and beverage additives.

  According to these inventions, the food of the present invention is based on the anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle enhancing action of the feed of the present invention used in the organisms exemplified above. The expression of the same action can be expected.

  The agent of the present invention used for the feed is, for example, as a dry powder of thorny throat, usually preferably 1 to 2000 mg / kg body weight, more preferably 5 to 1000 mg / kg body weight, more preferably 10 to 500 mg per day. / Kg body weight is administered. For example, the dry powder of Togedokoro is added and mixed into the raw material of the artificial blended feed to be used for the target organism, or mixed with the powdered raw material of the artificial blended feed and then further mixed with the other raw materials. It can be carried out. Further, the content of the agent of the present invention in the feed is not particularly limited, and may be set as appropriate according to the purpose. For example, as a dry powder of thorny heart, in the feed, preferably 0.001 to 50. % By weight, more preferably 0.003 to 20% by weight, and even more preferably 0.05 to 10% by weight.

  There is no particular limitation on the method for producing the feed of the present invention, and the blending may be similar to that of general feed, and the active ingredient according to the present invention may be contained in the produced feed. A biological rearing agent can be prepared in the same manner.

  In addition, as a processed product derived from staghorn used for the food or feed of the present invention, a water-containing ethanol extract of swordfish is particularly preferably exemplified. The hydrous ethanol extract is a novel composition, and the food or feed containing the extract becomes a novel food or feed.

  According to the present invention, a medicament containing the agent of the present invention (hereinafter sometimes referred to as the medicament of the present invention) is provided. The medicament of the present invention is effective in, for example, treatment or prevention of chronic fatigue syndrome, nourishing tonic when the body feels heavy, and nutritional supplementation during physical fatigue due to its anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle enhancing action. Useful. It is also effective in increasing the endurance and muscles of athletes. In this case, the dosage form may be formulated by mixing the agent of the present invention with other drugs, or may be formulated separately and taken simultaneously.

  Examples of the medicament of the present invention include those prepared by combining the agent of the present invention with a known pharmaceutical carrier. In the present specification, the drug includes quasi drugs. In addition, the medicament of the present invention may exhibit other ingredients that can be used for the same use as the active ingredient of the present invention, that is, anti-fatigue action, endurance enhancing action, nourishing tonic action or muscle enhancing action. It can also be used in combination with other known ingredients, and there is no particular limitation. For example, garlic extract, suppon extract, royal jelly, ginseng, taurine, cordyceps, indian oyster, viper, coenzyme Q10, α-lipoic acid, Usutaketake And various amino acids.

  The production of the medicament of the present invention is usually carried out by blending Tedokoro or a processed product derived from Tedokoro with a pharmaceutically acceptable liquid or solid carrier, and optionally, a solvent, a dispersant, an emulsifier, a buffer, a stabilizer. Add excipients, binders, disintegrants, lubricants, etc. to form solids such as tablets, granules, powders, powders, capsules, etc., usually liquids, suspensions, emulsions, etc. Can do. Moreover, it can also be used as a dried product which can be made liquid by adding an appropriate carrier before use, and other external preparations.

  The pharmaceutical carrier can be selected depending on the administration form and formulation form of the medicine. In the case of an oral preparation comprising a solid composition, it can be a tablet, pill, capsule, powder, fine granule, granule, etc., for example, starch, lactose, sucrose, mannitol, carboxymethylcellulose, corn starch Pharmaceutical carriers such as inorganic salts are used. In preparation of the oral preparation, a binder, a disintegrant, a surfactant, a lubricant, a fluidity promoter, a corrigent, a colorant, a fragrance and the like can be further added. For example, in the case of a tablet or pill, if desired, it may be coated with a sugar coating such as sucrose, gelatin or hydroxypropylcellulose, or a film of a gastric or enteric substance. In the case of an oral preparation composed of a liquid composition, it can be a pharmaceutically acceptable emulsion, solution, suspension, syrup, etc., for example, purified water, ethanol, olive oil or the like is a carrier. Used as Further, if desired, auxiliary agents such as wetting agents and suspending agents, sweetening agents, flavoring agents, preservatives and the like may be added.

  On the other hand, in the case of a parenteral agent, in accordance with a conventional method, Tedokoro or a processed product derived from Tedokoro is used as a diluent, distilled water for injection, physiological saline, aqueous glucose solution, vegetable oil for injection, sesame oil, peanut oil, soybean oil, corn It can be prepared by dissolving or suspending in oil, propylene glycol, polyethylene glycol or the like, and adding a bactericidal agent, stabilizer, isotonic agent, soothing agent, etc., if necessary. In addition, a solid composition can be produced and dissolved in sterile water or a sterile solvent for injection before use.

  External preparations include solid, semi-solid or liquid preparations for transdermal administration or transmucosal (oral or intranasal) administration. Also included are suppositories and the like. For example, emulsions such as emulsions and lotions, tinctures for external use, liquid preparations such as liquids for transmucosal administration, ointments such as oily ointments and hydrophilic ointments, transdermal administration such as films, tapes, and poultices Or a patch for transmucosal administration.

  The medicaments in the various preparation forms as described above can be appropriately produced by conventional methods using known pharmaceutical carriers and the like. In addition, the content of the active ingredient in such a medicament is not particularly limited as long as the active ingredient can be administered within the dosage range described below in consideration of its administration form, administration method and the like. . There is no particular limitation on the content of the squirrel or processed product derived from the stalk in the medicament of the present invention, but for example, when using a water-containing ethanol extract of stalks, usually 1 to 100% by weight in terms of dry weight, preferably It is about 5 to 95% by weight, more preferably about 10 to 90% by weight.

  The medicament of the present invention is administered by an appropriate administration method according to the preparation form. There is also no particular limitation on the administration method, and for example, it can be administered by internal use, external use or injection. When the therapeutic agent or prophylactic agent of the present invention is administered by injection, it can be administered, for example, intravenously, intramuscularly, subcutaneously or intradermally. When administering by external use, for example, it may be administered by an appropriate administration method as an external preparation such as a suppository.

  The dosage of the medicament of the present invention is appropriately set according to the formulation form, administration method, purpose of use and age, weight, and symptom of the patient to whom the medicament is administered, and is not constant. In general, it is preferably a dose of 1 to 2000 mg / kg body weight, more preferably 5 to 1000 mg per day for an adult, in a dosage of a dried product of Togedokoro or a processed product derived from Todedokoro, for example, a water-containing ethanol extract of Togedokoro contained in the preparation. / Kg body weight, more preferably 10 to 500 mg / kg body weight. Of course, since the dosage varies depending on various conditions, an amount smaller than the above-mentioned dosage may be sufficient or may be necessary beyond the range. Administration may be carried out in a single dose or divided into several doses within a desired dose range. The administration period is also arbitrary. In addition to the oral administration of the medicament of the present invention, it can be added to any food and taken daily.

  In addition, as a processed product derived from Tedokoro used in the medicament of the present invention, a water-containing ethanol extract of Tedokoro is particularly preferably exemplified. The hydrous ethanol extract is a novel composition, and a medicine containing the extract is a novel medicine.

  In addition, as another aspect of the present invention, there is provided a fatigue recovery or prevention method, endurance enhancement method, nutrition tonic method, or muscle enhancement method, which includes a step of administering Tedokoro or a processed product derived from Tedokoro. Here, the administration of Togedokoro or the processed product derived from Togedokoro can be administered as the food of the present invention or the pharmaceutical of the present invention. In addition, about the conditions of administration of a togedokoroh or a processed product derived from a togekoro, it can implement according to the indication of the administration of the said pharmaceutical.

  Further, as another aspect of the present invention, there is provided a spine or a spine-derived treated product for use in a method for recovering or preventing fatigue, a method for enhancing endurance, a method for enhancing nutrition, or a method for strengthening muscles. The Tedokoro or processed product derived from Tedokoro can be used as the food of the present invention or the pharmaceutical of the present invention.

  Moreover, as another aspect of the present invention, there is provided the use of Togedokoro or Togedokoro-derived processed products in the manufacture of an anti-fatigue agent, endurance enhancing agent, nourishing tonic or muscle enhancing agent. In addition, the present invention provides the use of Tedokoro or a processed product derived from Tedokoro in the manufacture of a food for anti-fatigue, endurance enhancement, nutrition tonic or muscle enhancement. In addition, the present invention provides the use of Tedokoro or a processed product derived from Tedokoro in the manufacture of a medicament for anti-fatigue, endurance enhancement, nutrition tonic or muscle enhancement.

  Moreover, as another aspect of the present invention, there is also provided a food or feed for anti-fatigue, for enhancing endurance, for enhancing nutrition, or for enhancing muscle, characterized by containing Tedokoro or a processed product derived from Tedokoro. Examples of the food or feed include food or feed with an indication that it is used for recovery or prevention of fatigue, endurance enhancement, nutrition tonic or muscle enhancement.

  No toxicity is observed in the toledokoro or the treated product derived from the toggedoro used in the present invention even when an effective amount for the expression of its action is administered to a living body.

Preparation of dry powder of Togedokoro About 370 kg of Togedokoro (Dioscorea esculenta) was purchased from a store in Okinawa Prefecture. What was sliced to about 5 mm was dried by heating at 60 ° C. for 21 hours. About 7 kg was treated with far-infrared (120 ° C., 7 to 8 minutes) and then pulverized to obtain 6 kg of powder.

Evaluation of anti-fatigue action of Togekokoro powder Male ICR mice were purchased from Japan SLC and used for experiments from 6 weeks of age after preliminary breeding. A test meal was prepared by mixing the powdered twigs prepared in Example 1 with standard feed CE-2 (manufactured by CLEA Japan, Inc.) at a rate of 1% (w / w). Prior to administration of the test meal, individuals capable of swimming for a predetermined time or more were selected in advance and equally distributed to each group. Thereafter, the swimming exercise time of the mice of the test group that freely ingested the test food and the control group that freely ingested the standard feed CE-2 were measured and compared on the 7th, 8th, and 9th days. The swimming exercise time was defined as the time from when the mouse was loaded with a weight of 1% of the body weight and forced swimming was started, and the head was completely submerged in water for 5 seconds after fatigue. The average value ± standard error (minutes) of 23 mice in each group is shown in Table 1. As a result, in the test meal administration group, the swimming time was extended from the seventh day after the start of administration. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

Preparation of Todokoro 70% (v / v) ethanol extract 200 g of dried Togedokoro powder prepared in Example 1 was added to 2 L of 70% (v / v) ethanol with stirring and stirred at 25 ° C. for 24 hours using a stirrer. Then, suction filtration was performed using a Buchner funnel (filter paper No. 2 manufactured by Whatman). The filtrate was dried with an evaporator to obtain 19.02 g of a 70% (v / v) ethanol extract. A 70% (v / v) ethanol extract was mixed with a standard powder feed CE-2 (manufactured by CLEA Japan, Inc.) to obtain a mixed feed equivalent of 1% (w / w) dried powder of lizards, and used as a test meal.

Evaluation of anti-fatigue action of 70% (v / v) ethanol extract of Togedokoro Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. The mice in the test group in this example were allowed to freely ingest the test meal prepared in Example 3 from one week before the swimming exercise time was measured. The results on the seventh day after the start of administration are shown in Table 2 as the mean value ± standard error (minutes) of 10 animals in each group. As a result, the swimming time was prolonged in the 70% (v / v) ethanol extract administration group. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

Preparation of acetone-extracted fraction from 70% (v / v) ethanol extract of Togedokoro Suspended by adding 300 mL of acetone to 5.031 g of 70% (v / v) ethanol extract of Togedokoro prepared in Example 3. Centrifugation was performed at 5600 × g for 20 minutes. The supernatant was dried with an evaporator to obtain 245 mg of a Tedokoro acetone extract. Acetone extract was mixed with standard powdered feed CE-2 (manufactured by CLEA Japan) to make the equivalent of 1% (w / w) dried powder of Togekokoro as test food.

Evaluation of anti-fatigue action of acetone-extracted fraction from 70% (v / v) ethanol extract of Togedokoro Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. The mice in the test group in this example were allowed to freely ingest the test meal prepared in Example 5 from one week before the swimming exercise time was measured. The results on the 8th day from the start of administration are shown in Table 3 as the average value ± standard error (minutes) of 9 animals in each group. As a result, the swimming time was prolonged in the acetone extract administration group. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

Biochemical evaluation of anti-fatigue action of Tedokoro powder Male ICR mice were purchased from Japan SLC Co., Ltd. and tested in the same manner as Example 2 in which Tedokoro powder was mixed with standard diet CE-2 at a rate of 1% (w / w). The meal was prepared and administered, and the day after the swimming experiment was conducted from the 7th day to the 9th day, that is, on the 10th day from the start of the test meal administration, the mouse was loaded with a weight of 1% (w / w) of body weight. Blood was collected after 10 minutes of forced swimming. The blood creatine kinase value was measured using Dry Chem (Fuji Film). In addition, a group that did not force swimming exercise was defined as an untreated group. The average value ± standard error (U / L) of each group is shown in Table 4. In the control group, the creatine kinase level was higher than that in the untreated group due to the accumulation of fatigue due to daily swimming exercise, whereas in the test food administration group, the value was similar to that in the untreated group.

Evaluation of anti-fatigue action of acetone-extracted fraction from 70% (v / v) ethanol extract of Togedokoro (dose dependence)
An acetone extract was prepared in the same manner as in Example 5. These were mixed with standard powdered feed CE-2 (manufactured by CLEA Japan) to prepare a test meal. The doses were equivalent to 3% (w / w) and 10% (w / w) mixed diets of Togedokoro dry powder, respectively. Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. The mice (2 groups) in the test group in this example were allowed to freely ingest the test meals (2 species) from one week before the swimming exercise time was measured. The control group received the standard powdered feed CE-2. The results from the 7th day to the 9th day from the start of administration are shown in Table 5 as the mean value ± standard error (min) of 8 to 10 animals in each group. As a result, swimming time was prolonged when the dose of acetone extract was increased. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

Evaluation of anti-fatigue action of acetone-extracted fraction from 70% (v / v) ethanol extract of Togedokoro (gavage administration)
An acetone extract was prepared in the same manner as in Example 5. This is dissolved in a small amount of ethanol (made by Kishida Chemical Co., Ltd.) to a predetermined concentration, and olive oil (made by Wako Pure Chemical Industries, Ltd.) is added so that the ethanol concentration becomes 10% (v / v). A homogeneous solution was prepared. As the vehicle, olive oil to which ethanol was added so as to be 10% (v / v) was used. Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. In the test group mice (group 2) in this example, the samples prepared as described above were treated on the first day, the first day, the second day, the third day, the sixth day, the seventh day, the eighth day. On day 9, gavage was administered. The results on day 9 are shown in Table 6 as the mean value ± standard error (minutes) of 6 to 10 animals in each group. As a result, when the acetone extract was administered by oral gavage, the swimming time was extended, and when the dose was increased, the swimming time was further extended. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

A tablet (250 mg / tablet) containing a dry powder of Togekokoro was prepared according to a conventional method with a formulation shown in Table 7 and using a tableting machine at a pressure of 1000 kg / cm ² during tableting. The mixing was divided into primary mixing and secondary mixing, followed by tableting.

A tablet (250 mg / tablet) containing dried powder of Togekokoro was prepared according to a conventional method with the formulation shown in Table 8 below and using a tableting machine at a pressure of 1000 kg / cm ² during tableting. The mixing was divided into primary mixing and secondary mixing, followed by tableting.

Preparation of Todokoro 60% (v / v) Ethanol Extract 800 g of dried Togedokoro powder prepared in Example 1 was added to 8 L of 60% (v / v) ethanol with stirring and stirred at 25 ° C. for 24 hours using a stirrer. Then, suction filtration was performed using a Buchner funnel (filter paper No. 2 manufactured by Whatman). The filtrate was dried with an evaporator to obtain 98.9 g of a 60% (v / v) ethanol extract.

Evaluation of anti-fatigue action of 60% (v / v) ethanol extract of Togedokoro Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. In the test group mice in this example, 60% (v / v) ethanol extract prepared in Example 12 was added 1.5% (w / w) (Tedokoro Powder 10) from 1 week before measuring swimming exercise time. % (W / w) equivalent) was allowed to freely ingest the test meal mixed with the standard powdered feed CE-2 (manufactured by CLEA Japan). The results on the 7th and 8th days from the start of administration are shown in Table 9 as the mean value ± standard error (minutes) of 15 animals in each group or 13 animals in each group. As a result, the swimming time was prolonged in the 60% (v / v) ethanol extract administration group. On the other hand, it was found that there were no abnormalities in changes in body weight and food intake during the administration period, and it was safe to take.

Effect of administration of Togedokoro powder on blood lactic acid level Test in which male ICR mice were purchased from Japan SLC and mixed with Standard Diet CE-2 at a rate of 3% (w / w) of Togedokoro Powder prepared in Example 1 A meal was prepared and administered. Swim until the head is completely submerged in the water for 5 seconds after getting tired on the 7th and 8th days. Immediately after the 5-minute swim exercise on the 9th day, blood is collected from the abdominal vena cava under anesthesia. The lactic acid level in blood was measured using a lactate measuring apparatus Lactate Pro (manufactured by Arkray). In addition, a group not performing swimming exercise was defined as an untreated group. The average value ± standard error (mmol / L) of each group is shown in Table 10. In the control group, the blood lactic acid level was high, whereas in the group with Tedokoro powder, it was lower than in the control group.

Effect of 60% (v / v) ethanol extract administration of Togedokoro on blood lactic acid level Male ICR mice were purchased from Japan SLC, and the 60% (v / v) ethanol extract prepared in Example 12 was 0. A test meal mixed with the standard meal CE-2 was prepared and administered at a rate of 5% (w / w) (corresponding to 3% (w / w) powder). A swimming exercise was performed in the same manner as in Example 14, and blood was collected immediately after 5 minutes of swimming exercise on the 9th day, and blood lactic acid level was measured. In addition, a group not performing swimming exercise was defined as an untreated group. The average value ± standard error (mmol / L) of each group is shown in Table 11. In the control group, the blood lactic acid level was high, whereas in the Tedokoro ethanol extract administration group, it was lower than the control group.

  Using an extrusion granulator (1.0 mm basket) with the formulation shown in Table 12 below, granules containing dry powder of Togekokoro were prepared according to a conventional method. That is, after kneading, granulating, and drying, the granules were sized to produce granules.

  Hard capsules containing Togekokoro dry powder were prepared according to a conventional method with the formulation shown in Table 13 below. That is, after mixing 1 and 2, it was filled to produce a hard capsule.

  Oval 6-type soft capsules containing dry powdered thorned corona were prepared according to a conventional method with the composition shown in Table 14 below. That is, after preparing a chemical solution, pulverizing, and defoaming, soft capsules were manufactured by filling and drying.

  After adding 800 g of dried Togekokoro powder to 8 L of 60% ethanol with stirring and stirring at 25 ° C. for 24 hours using a stirrer, 7.38 L of filtrate was obtained by suction filtration. Of these, 1 g of emulsifier (SY Glister MM-750: Sakamoto Yakuhin Kogyo Co., Ltd.) was added to 100 mL, and the mixture was concentrated and the solvent was removed with a rotary evaporator. Water was added to the extract from which the solvent had been removed, and the resulting mixture was adjusted to 100 mL. A drink containing a spidered emulsion was prepared according to a conventional method with the formulation shown in Table 15 below. That is, after mixing, a drink was produced by filling and sterilizing.

Effect of long-term administration of tedgekoro powder and extract and regular exercise 1
Male BALB / c mice were purchased from Japan SLC and used for experiments from 7 weeks of age after preliminary breeding. A test meal was prepared by mixing the powdered twigs prepared in Example 1 with standard feed CE-2 (manufactured by CLEA Japan, Inc.) at a rate of 1% (w / w). In addition, a test meal prepared by mixing the 60% (v / v) ethanol extract prepared in Example 12 with the standard powder sample CE-2 was also used. Prior to administration of the test meal, individuals capable of swimming for a predetermined time or more were selected in advance and equally distributed to each group. After that, a test group that freely ingests the test meal and a control group that freely ingests the standard feed CE-2 are set, and the first day of the test meal administration is the first day. Swim exercise was performed until it submerged for 5 seconds. The time from fatigue to the first to fifth weeks until the head completely submerged in water for 5 seconds was measured. Swimming exercise was performed in the same manner as in Example 2. Table 16 shows the average value ± standard error (minutes) of 11-15 animals in each group. Compared to the control group, the swimming time was increased in the test meal mixed with 1% (w / w) of Togekokoro powder and the test meal administered with 60% (v / v) ethanol extract. On the other hand, it was found that there was no abnormality in the change in food intake during the administration period, and it was safe to take.

Effect of long-term administration of togekokoro powder and extract and regular exercise 2
Male BALB / c mice were purchased from Japan SLC and used for experiments from 7 weeks of age after preliminary breeding. The same experiment as in Example 20 was performed, and the blood was lethal and lethal under anesthesia the day after the swimming exercise was carried out until the head was completely submerged in water for 5 seconds after the 8th week of administration of the test meal. The gastrocnemius and soleus muscles of the right hind limb were removed and weighed. In addition, fat around the testis was extracted and weighed. The average of 9-15 animals in each group is shown in Table 17. Compared to the control group, increased muscle mass was observed in the 1% (w / w) and 60% (v / v) ethanol extract-administered groups. On the other hand, the amount of fat was decreased in the groups administered with 1% (w / w) and 60% (v / v) ethanol extract of Togedokoro powder.

［対照例１］

[Control Example 1]

Preparation of Ginenjo dry powder 1 kg of Ginenjo (Dioscorea japonica) (produced in Kyotango) was sliced in a thickness of 2 to 3 mm with skin. The sliced material was dried at 60 ° C. for 5 hours and then pulverized with a small ultra-high speed pulverizer (manufactured by Osaka Chemical Co., Ltd.) to obtain 214.39 g of Ginenjo powder.
[Control Example 2]

Evaluation of anti-fatigue action of Ginenjo administered mice Male ICR mice were purchased from Japan SLC, and the swimming time was measured in the same manner as in Example 2. The mice in the test group in this example were allowed to freely ingest the test meal prepared in Control Example 1 from one week before the swimming exercise time was measured. The results on the 7th day from the start of administration are shown in Table 18 as the average value ± standard error (minutes) of 9 animals in each group. As a result, no increase in swimming time was observed in the mice administered Ginenjo.

  INDUSTRIAL APPLICABILITY According to the present invention, anti-fatigue agent, endurance enhancer, nourishing tonic agent or muscle enhancer containing Tedokoro or Tedokoro-derived processed product as an active ingredient, food, feed or medicine containing the agent, and useful as the agent An extract from Togekokoro and a method for producing the extract are provided. The anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer is useful not only for improving the ability of athletes, but also as a material for foods or medicines that have a fatigue recovery effect in social life.

Claims (12)

  An anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer containing Togedokoro or a processed product derived from Togedokoro as an active ingredient.   The anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer according to claim 1, wherein the processed product derived from Togekokoro is a dry powder, water, ethanol, hydrous ethanol or acetone extract, or a fraction obtained from the extract. .   A food or feed comprising the anti-fatigue agent, endurance enhancer, nourishing tonic or muscle enhancer according to claim 1 or 2.   A medicament comprising the anti-fatigue agent, endurance enhancer, nutrition tonic or muscle enhancer according to claim 1 or 2.   A method for producing an extract, comprising a step of extracting Tedokoro with water, ethanol, hydrous ethanol or acetone.   The method according to claim 5, wherein the hydrous ethanol is 1 to 85% (v / v) hydrous ethanol.   An extract obtainable by the method of claim 5 or 6.   A food or feed containing the extract according to claim 7.   The pharmaceutical containing the extract of Claim 7.   A method for recovering from fatigue or preventing fatigue, a method for enhancing endurance, a method for strengthening nutrition, or a method for strengthening muscles, which includes a step of administering Togedokoro or a processed product derived from Togedokoro.   Togedokoro or Togedokoro-derived treated product for use in fatigue recovery or prevention method, endurance enhancement method, nutrition tonic method or muscle enhancement method.   A method for producing a food containing Togekokoro or a processed product derived from Togedokoro, comprising a step of indicating that the product is used for recovery from fatigue or prevention, endurance enhancement, nourishment tonic, or muscle enhancement in production.