KR100679698B1 - An extract of Pterocarya paliurus with blood sugar level-lowering activity and functional food comprising the same - Google Patents

Abstract

The present invention relates to a green current extract having a hypoglycemic activity extracted from a new material, Pterocarya paliurus , a functional food containing the same, and a hypoglycemic tea composition using the green current.

According to the present invention, due to the excellent hypoglycemic activity of the green current or its extract can be effectively used in the preparation of tea compositions and various types of functional foods that can help prevent and treat diabetes.

Green current, green tea, diabetes, hypoglycemic

Description

An extract of Pterocarya paliurus with blood sugar level-lowering activity and functional food comprising the same}

1 is a photograph of the blue current used in the blue current extract of the present invention.

Figure 2 is a graph showing the hypoglycemic effect of the green current difference and the extract of the present invention.

The present invention relates to a new plant extract and tea composition having a hypoglycemic activity, and more specifically, a green current extract having a hypoglycemic activity extracted from a blue current, a functional food containing the same, and a hypoglycemic tea composition using a blue current It is about.

Diabetes is a disease that causes acute symptoms due to significant hyperglycemia or ketoacidosis or various chronic and systemic metabolic disorders due to persistent hyperglycemic conditions or decreased glucose tolerance. Diabetes mellitus is caused by genetic congenital factors and acquired factors due to the environment such as diet and exercise, and because both affect each other, various forms of diabetes mellitus and disease progression are extended. The frequency of occurrence is steadily increasing due to various changes in eating and eating habits.

Until now, only insulin has been selected as the most ideal and effective drug for the treatment of diabetes. However, it has a short duration, does not show oral action, and has to be used as an injection only. Even if administered, blood sugar drops and symptoms of hypoglycemia occur. In severe cases, if not treated, it can cause convulsions, unconsciousness, brain damage, and death.

In addition, the most typical to be used for the purpose of treating diabetes by controlling the blood sugar drop is tolbutamide, when using tobutamide as a diabetes treatment may cause pancreatic cancer, pain, blood stains, etc. If you do not have a blind spot that can pose a fatal risk to life, and the effect of lowering blood sugar appears quickly, but the effect is not persistent.

Approaches for the treatment of diabetes described above are Western medical methods for achieving artificial blood sugar drop, and the expectations of natural physiology are insufficient. Therefore, there is an urgent need for the development of anti-diabetic agents using herbal extracts that can reduce these side effects and have an effect on lowering blood sugar.

Green current (Pterocarya paliurus (Batal.) Or Cyclocarya paliurus (Batal.)) Is a deciduous tree found in a limited mountainous region of the Yangtze River basin in China. This leaf tea has been used not only as a drink, but also for various symptoms such as fatigue, abdominal pain, diarrhea, hemostasis, and itching.

In recent years, a survey of the disease in the area has revealed that relatively few people with hyperglycemia and diabetes have investigated the cause and found that it is caused by this plant. Investigations in China have shown that the plant protects damaged insulin-producing cells, promotes insulin secretion, and promotes the use of glucose in the blood as an energy source.

In order to treat and prevent diabetes, various medicines and natural foods are sold and developed. Among them, oral hypoglycemic agents, insulins, and complications are used. In this case, hypoglycemia, allergies, hyperlipidemia, polyuria, etc. There are many examples of side effects.

As an example of food, products using various materials have been recently developed, and the representative patent applications are as follows. Anti-diabetic reproductive type tea composition (Reg. No. 10-0325584) added with raw materials such as germinated brown rice, germinated barley, germinated black soybean and chelate zinc (Reg. No. 10-0325584), and functional antidiabetic beverage using silkworm extract extracted from silkworm powder ( Registration No. 10-0354151), manufacturing method of high functional GABA tea (Reg. No. 10-0367055), pulse door extract with antidiabetic effect (Application No. 1020020018169), composition of anti-diabetic patient using silkworm extract (Application No. 1020020018118) , Composition for preventing and treating diabetes containing polysaccharide fraction extract isolated from caterpillar Cordyceps sinensis (application number 1020020008409), health food composition having a hypoglycemic effect using herbal medicines such as turmeric, creation, astragalus, triticale Japanese Patent Application No. 1996-003608), anti-diabetic composition containing mulberry leaves and green tea (Publication No. 2003-0084021), and the like. However, there was no example of using a blue current.

Accordingly, the present inventors have made intensive studies to overcome the problems of the prior art, and confirmed that the extract extracted from the new material, the blue current, exhibits an excellent hypoglycemic effect and completed the present invention.

Therefore, the main object of the present invention is to provide a composition for lowering blood glucose, which contains a blue current extract as an active ingredient.

Another object of the present invention to provide a functional food for lowering blood sugar using the current extract.

Still another object of the present invention is to provide a tea composition for lowering blood sugar using a green current leaf.

In order to achieve the object of the present invention, the present invention provides a composition for lowering blood glucose, which contains a extract of Pterocarya paliurus as an active ingredient.

In the present invention, Pterocarya paliurus is a deciduous tree seen in a limited mountainous region of the Yangtze River basin in China. Tea made has been used not only as a beverage but also for various symptoms such as fatigue improvement, abdominal pain, diarrhea, hemostasis, itching, etc., for the first time in the present invention, the green current has been found to have a blood sugar-lowering ability. The blue current is listed as the main raw material in the food raw material of the Republic of Korea Food Code, its safety is recognized, and proved to be non-toxic in Experimental Example 1 below.

In the present invention, preferably, the green current extract is characterized by extracting the leaves, stems or mixed portions of the green current with water or a hydrophilic organic solvent or a mixed solvent thereof, and more preferably the leaves of the green current. It is put in water to provide a composition characterized in that the hot water extraction by heating at 50 ~ 100 ℃.

In the present invention, the green current extract may be in a liquid, paste or solid form, but preferably, the composition is characterized in that the powder is made by filtering the extract and evaporating and drying the solvent.

In order to achieve the other object of the present invention, the present invention provides a functional food for hypoglycemic containing as an active ingredient of the current extract of the present invention.

In the present invention, the functional food includes any food form that can be ingested orally, but provides a functional food, characterized in that the beverage, pills, granules, tablets or capsules form.

In order to achieve another object of the present invention, the present invention provides a hypoglycemic tea composition containing 30 to 100% by weight of the green current leaf.

In the present invention, the tea composition may further include other secondary tea materials to improve the palatability and functionality in addition to the green current leaves, but preferably 30 to 99% by weight of the green current leaves and jasmine tea, green tea, powder and chrysanthemum. It provides a hypoglycemic tea composition comprising 1 to 70% by weight of any one or more mixtures selected from the group consisting of.

Looking at the present invention in more detail as follows.

In the present invention, the entire green current tree may be used, but from the viewpoint of its physiological action, it is particularly preferable to use above ground portions (leaves and stems), and among them, it is preferable to use the green current leaves. In addition, in this invention, a clean current extract shall also contain the extract obtained by an extraction process, the dilution or concentrate of an extract, the dried material obtained by drying an extract, or any of these modifiers or refined products.

The green current extract of the present invention is obtained by using the leaves, stems, or mixed portions of the green current as an extraction material, and adding the water or a hydrophilic organic solvent or a mixed solution thereof at room temperature to a temperature below the boiling point of the solvent. It can be obtained by extraction using a device.

The water which can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, and various kinds of treatments thereof. Treatments to water include, for example, purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of penetration pressure, buffering, and the like. Therefore, water which can be used as the extraction solution in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

As said hydrophilic organic solvent, For example, C1-C5 lower alcohols, such as methanol, ethanol (alcohol), propyl alcohol, isopropyl alcohol; Lower aliphatic ketones such as acetone and methyl ethyl ketone; C2-C5 polyhydric alcohols, such as 1, 3- butylene glycol, a propylene glycol, and glycerin, etc. are mentioned, The mixed solution of these hydrophilic organic solvents, and water can be used. In the case of using a mixed solvent of water and a hydrophilic organic solvent, 1 to 90 parts by weight for 10 parts by weight for lower alcohol, 1 to 40 parts by weight for 10 parts by weight of water for lower aliphatic ketone, In the case of polyhydric alcohol, it is preferable to add 10-90 weight part with respect to 10 weight part of water. The spirits include fermented spirits (grain grains), which are fermented by microorganisms or enzymes as grains, and synthetic spirits produced using ethylene obtained from petroleum or coal as raw materials.

Specifically, the dried and ground products of the green current leaves are put into a treatment tank filled with an extraction solvent, and left to stand for 30 minutes to 2 hours with occasional stirring as necessary to elute the soluble components, and then filtered to remove the solids. The extract is obtained by removing the extraction solvent from the obtained extract and drying. The amount of the extraction solvent is preferably 5 to 15 times the weight ratio (weight ratio) of the extraction raw material, and the extraction conditions are usually about 1 to 4 hours at 50 to 100 ° C. when water is used as the extraction solvent. Moreover, when using a mixed solvent of water and ethanol (for example, 30-80 weight% hydrous ethanol) as an extraction solvent, it is about 30 minutes-about 4 hours at 40-80 degreeC normally.

Removal of the solvent from the extract obtained by filtration or centrifugation yields a concentrate in the form of a paste. When dried again, a solid extract is obtained, and when it is powdered, an extract in powder form is obtained. However, the extract of the Astragalus leaf used in the present invention does not need to be a solid extract, and may be in the state of the extract or the concentrate thereof. These may be used after purification by methods such as activated carbon treatment, adsorptive resin treatment, ion exchange resins, liquid-liquid countercurrent distribution, etc., unless they prevent the achievement of the object of the present invention.

Further, in the functional food for hypoglycemic function containing the blue-current extract of the present invention as an active ingredient, the cleansing extract extracted as described above, the extract obtained by isolating the extract, the crude tablet of each manufacturing step, and a mixture thereof The same can also be used. It can be added to the food as it is to produce the functional food for hypoglycemic of the present invention.

Since the functional food of the present invention thus obtained can be consumed on a daily basis, a high blood sugar lowering effect can be expected, which is very useful. In addition, in this invention, a functional food means what includes a general food, a health food, a health functional food, quasi-drugs, a medicine, etc. ingested orally widely.

Although the addition amount of the blue current extract in such a food cannot be uniformly prescribed | regulated depending on the kind of target food, what is necessary is just to add it in the range which does not impair the original taste of a food, and it is normally 0.01-50 weight weight with respect to a target food. %, Preferably it is the range of 0.1-20 weight%. In addition, in the case of food in the form of a pill, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 5 to 100% by weight.

In addition, it is appropriate to administer the green current extract, which is an active ingredient of the functional food of the present invention, so that the daily intake amount of adult is 1 to 5000 mg. In addition, the dosage can be appropriately increased or decreased depending on age, symptoms, and the like.

Functional foods to which the hypoglycemic activity of the present invention can be applied are not particularly limited. For example, beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, etc .; ; Noodles such as soba (buckwheat noodles), udon noodles, somen noodles, dumplings, steamed dumplings, medium noodles, and instant noodles; Confectionery such as candy, candies, gum, chocolate, sweets, snacks, biscuits, jelly, jams, pictures, baked sweets and bread; Aquatic products such as crab, salmon, clams, tuna, sardines, shrimp, bonito, mackerel, whale, oyster, saury, squid, scallop, scallops, abalone, sea urchin, Ikra, and Obungigi; Fish and livestock processed foods such as meat, ham and sausage; Fats and oils such as dairy products such as processed oil and fermented milk, salad oil, fried oil, margarine, mayonnaise, shortening, whip cream and dressing; Seasonings such as sauces and seasonings; Curry, sheet, chicken egg rice bowl, porridge, rice porridge, Chinese rice bowl, pork cutlet rice bowl, fried rice bowl, eel rice bowl, hashed rice, oden, mapo tofu, beef rice bowl, meat sauce, egg soup, omurice, dumpling, Retorto pouch foods such as steamed buns, hamburgers and meatballs; Health and nutritional supplements in various forms; Medicines such as tablets, capsules, drinks, troches, quasi-drugs, and the like, and the like, and these may be added together with auxiliary raw materials or additives commonly used in the purification.

In addition, the raw material or additives are not particularly limited, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, loxoside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascor Binic Acid, d1-α-Tocopherol, Sodium Elysolevin, Glycerine, Propylene Glycol, Glycerin Fatty Acid Ester, Polyglycerin Fatty Acid Ester, Sucrose Fatty Acid Ester, Sorbitanic Fatty Acid Ester, Arabian Gum, Colorinane, Casein, Gelatin, Pectin, Agar, Vitamin Bs, nicotinic acid amide, calcium panthenate, amino acids, calcium salts, pigments, flavors, preservatives and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

Example 1 Preparation of Blue Current Dry Leaves

Foreign matters were selected from the leaves of blue current harvested from Xiushan, Jiangxi Province, China, and dried to make the moisture content less than 10%.

Example 2. Preparation of Blue Current Extract

The blue current dried leaf prepared in Example 1 was boiled at 100 ° C. for 30 minutes to 2 hours with water of 10 to 30 times the weight, thereby preparing a blue current extract. If necessary, after filtration, water is evaporated and freeze-dried to powder. In addition, various solvents such as alcohol may be used for effective extraction of the active ingredient.

Example 3. Preparation of Blue Current Difference

30 to 100% by weight of the dry current leaf dried in Example 1, 0 to 10% by weight of jasmine tea, 0 to 10% by weight of green tea, 0 to 10% by weight, 0 to 10% by weight of chrysanthemum, to improve palatability and functionality Extract by adding% to make drinkable form (tea bag, leaf tea, etc.).

Example 4. Preparation of Beverage Using Green Current Extract

The blue current extract prepared in Example 2 is 0.001 to 10% by weight, 2 to 30% by weight of sugar, 0.01 to 0.5% by weight of citric acid or malic acid, 0.01 to 0.5% by weight of fragrance, and the remainder is purified water. To prepare.

Example 5 Preparation of Rings Using Blue Current Extracting Powders

A ring containing 5 to 100% by weight, 0 to 5% by weight of Cordyceps sinensis, 0 to 5% by weight of honey, etc., is prepared in the blue current extraction powder prepared in Example 2.

Example 5 Preparation of Tablets Using Blue Current Extracting Powders

5 to 70% by weight, 10 to 40% by weight of dextrin, 10 to 40% by weight, 2 to 10% by weight of glycerin fatty acid ester, and the like, were purified by a conventional tableting machine. To prepare.

Example 6 Preparation of Capsule Using Blue Current Extracting Powder

5 to 70% by weight of the current extract powder prepared in Example 2, 10 to 30% by weight cornstarch, 10 to 40% by weight lactose, 2 to 10% by weight calcium lactate, 2 to 10% by weight hydroxypropyl cellulose After blending, capsules are prepared using hard gelatin capsules.

Experimental Example 1. Toxicity Test

The blue current extract prepared in Example 2 was administered to mice to observe the toxicity. The rats used in the experiment were 36 male and female Wistar rats (80-90 g in weight), which were observed for 1 week before the experiment, and removed the bad subjects. During the experiment, diet was controlled by body weight and water was supplied freely. Males and females were separated and bred, and the experiments were randomly divided into three groups, namely, 12 mice, which were divided into three groups, a control group, a low concentration group (7.2 g / kg) and a high concentration group (21.6 g / kg). The experimental period was 12 weeks in total, and the frequency of administration was once a day, 6 days a week. Blood was collected from the abdominal aorta and analyzed. The results are shown in Table 1 and Table 2.

The changes in the hematological indexes of the rats in each group at the end of the administration stage (X ± SD, n = 12) RBC (× 10 ¹² / L) HB (g%) WBC (× 10 ⁹ / L) DC N (%) L (%) BPC (× 10 ⁹ / L) Control 8.6 ± 1.1 11.9 ± 0.7 12.1 ± 3.0 42.9 ± 7.3 56.2 ± 9.7 49.3 ± 10.4 Low concentration group (7.2 g / kg) 8.1 ± 0.9 10.3 ± 0.5 12.7 ± 3.6 47.1 ± 18.9 52.2 ± 8.1 57.4 ± 11.3 High concentration group (21.6g / kg) 9.2 ± 1.3 12.8 ± 0.9 10.4 ± 2.8 40.3 ± 6.8 57.9 ± 10.4 46.6 ± 9.7

The changes in the blood biochemical criteria of the rats in each group at the end of the administration stage (X ± SD, n = 12) BUN (mmol / L) Cr (μmol / L) ALT (μ) TP (g / L) ALB (g / L) T-CHO (mmol / L) Control 0.67 ± 0.04 297.8 ± 36.4 30.4 ± 5.1 73.6 ± 6.8 30.8 ± 2.7 1.08 ± 0.19 Low concentration group (7.2 g / kg) 0.57 ± 0.06 266.4 ± 32.5 34.0 ± 6.7 70.2 ± 7.1 28.3 ± 2.3 0.92 ± 0.17 High concentration group (21.6g / kg) 0.64 ± 0.04 273.9 ± 30.4 27.3 ± 4.8 68.9 ± 6.2 27.4 ± 3.1 0.96 ± 0.20

RBC: red blood cell count, HB: hemoglobin quantity, WBC + DC: white blood cell count and its classification, BPC: blood platelet count, BUN: urea nitrogen, Cr: creatinine, ALT: alanine transaminase, TP: total protein, ALB : albumin, T-CHO: total cholesterol

As shown in the above experimental results, even if administered in a concentration of 30 to 90 times the daily intake in terms of the concentration in humans for 12 weeks, there was no significant difference between the control group and the administration group can be concluded that there is no particular toxicity.

Experimental Example 2 Glucose Drop Test

In order to test the hypoglycemic effect of the green current extract of the present invention, 30 of 90 patients before and after the age of 50, the current of the green current leaf tea prepared in Example 1 three times a day, 3g maximum in 100ml of water For 5 minutes to drink and drink, 30 people made a ring made of 1 g of the green current extraction powder prepared in Example 2 to be ingested three times a day, and the remaining 30 people were not ingested blue current tea or pills. The experiment was conducted for a total of 4 weeks, and blood sugar analysis was performed on an empty stomach, 2 hours after a meal, and 24 hours after a meal.

The results are shown in Table 3.

Blood sugar before and after treatment (X ± SD, n = 100) car ring Silent group Drinking group No ingestion Intake group Fasting Blood Sugar (mmol / L) 10.86 ± 2.49 9.37 ± 2.74 ^* 10.86 ± 2.49 9.12 ± 2.56 ^* Blood sugar 2 hours after meal 14.51 ± 4.08 12.09 ± 4.48 ^* 14.51 ± 4.08 11.83 ± 3.27 ^* Blood sugar 24 hours after meals 28.12 ± 8.66 20.78 ± 9.63 ^* 28.12 ± 8.66 20.54 ± 5.36 ^*

 (*: Significantly different at P <0.01)

As can be seen from the above results, the fasting blood sugar, the blood sugar at 2 hours after a meal, and the blood sugar at 24 hours after a meal when the green current difference or the extract of the present invention are all so as to show a significant difference compared to the control group. It can be seen that the decrease.

As described above, according to the present invention, by using the blue current as a new material, the blood glucose lowering tea composition and various types of functional foods that can help prevent or treat diabetes due to the excellent hypoglycemic ability of the blue current or its extracts It can be effectively used in the preparation of the.

Claims (5)

Hypoglycemic composition comprising a green current (Pterocarya paliurus) extract as an active ingredient. [Claim 2] The composition for lowering blood sugar of claim 1, wherein the green current extract extracts leaves, stems, or mixed portions of the green current with water or a hydrophilic organic solvent or a mixed solvent thereof. The composition for lowering blood sugar according to claim 1, which is a functional food in the form of a beverage, pills, granules, tablets or capsules. Tea composition for hypoglycemic containing 30-100% by weight of the green current leaf. The blood glucose lowering tea composition according to claim 4, comprising 30 to 99% by weight of the green current leaf and 1 to 70% by weight of any one or more mixtures selected from the group consisting of jasmine tea, green tea, powder and chrysanthemum.

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