CN1460435A - Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method - Google Patents
Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method Download PDFInfo
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- CN1460435A CN1460435A CN03128111A CN03128111A CN1460435A CN 1460435 A CN1460435 A CN 1460435A CN 03128111 A CN03128111 A CN 03128111A CN 03128111 A CN03128111 A CN 03128111A CN 1460435 A CN1460435 A CN 1460435A
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Abstract
The present invention uses the oleanolic acid extracted from ligustrum fruit and zymolyzed dried powder made up by using silkworm chrysalis as main raw material, and adds the auxiliary materials of excipient and vitamins and utilizes a certain preparation process to obtain the invented liver-protecting functional food.
Description
Technical field
The present invention adopts modern biochemical technology, and the enzymolysis dry powder that oleanolic acid that extracts with the fruit of glossy privet and silkworm chrysalis make is as primary raw material, and preparation has the functional food that reduces transaminase, liver protection function.
Background technology
China is the heavy epidemic-stricken area of hepatitis sick district occurred frequently, especially virus B hepatitis (abbreviation hepatitis B).Hepatitis B is the formidable enemy of harm humans health, also is one of disease of harmfulness maximum in China's infectious disease.It is reported, China has hepatitis carrier 1.2 hundred million people (wherein 1/4 people can develop into the chronic hepatitis disease eventually) approximately, about 1,200 ten thousand examples of chronic hepatitis patient, wherein annual because of the dead number of hepatopathy reaches 300,000, and 40% mother who carries hepatitis B can give the baby with viral communication.Developing the medicine and the functional food with liver protection effect that hepatitis B there are practical curative effect, is a major issue that brings benefit to the people, and also is medical and health worker's a very urgent strategic task.The functional food that present China has liver protection effect is of less types, these functional food or compound product, or the not bright food of action component, though have liver protection effect through human body or zoopery proof, but the structure of still indeterminate active ingredient with this function or function factor and content standard thereof, detection method, can only carry out functional detection, and functional detection is subjected to the influence of factors such as time, funds.As following functional food for protecting liver: compound " Xiling " Medicinal granules is mainly mixed by rabdosia lophanthide, Black Ganoderma, gynostemma pentaphylla, Poria cocos, dried longan pulp, the fruit of Chinese wolfberry, mushroom, white fungus etc., newborn pigeon health spirits is to be main spice with squab, be equipped with Chinese herbal medicines such as the fruit of Chinese wolfberry, red date, living lalang grass rhizome, make through baking, three grades of immersions, sedimentation and filtrations, it is to be primary raw material with the loach enzymolysis protein that lucky star board liver protects capsule, though above-mentioned functions food has the effect of protecting liver, lowering enzymes, but ubiquity complicated component, the shortcoming that active ingredient is not clear can only belong to second generation functional food.And the clear and definite functional food of active ingredient structure and content, promptly third generation functional food is less in the research and development of China, lags behind America and Europe and Japan.Exploitation of China's functional food is compared with advanced international standard for this and there is a big difference, is unfavorable for finding one's way into world market.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide that a kind of active ingredient structure is clear and definite, liver protection effect is definite, dosage and formulation is stable, the significant functional food for protecting liver of prevention effect.Its active ingredient is the known oleanolic acid of chemical constitution (structural formula is as follows), and is equipped with the silkworm chrysalis enzymolyzing powder as nourishing additive agent.Oleanolic acid has stable morphology in this functional food, content standard is clear and definite, and detection method is simple and easy to do.
The objective of the invention is to realize: 1. the dried fruit of glossy privet is pulverized with pulverizer, extracted 2～3 times with 60～80% alcohol refluxs then, each 1～3 hour, filter merging filtrate by following technical proposals; 2. add H
2SO
4To the final mass percent concentration be 5～10%, stir, back flow reaction 2～4 hours reclaims ethanol; 3. sediment is washed till filtrate and becomes neutral with the NaOH solution washing of an amount of pH8～9, adds hot water again and fully washs, and filters, and gets crude product, activated carbon decolorizing; 4. 90～99% ethyl alcohol recrystallizations are 1～2 time, get oleanolic acid; 5. after fresh tussah chrysalis being cleaned, be twisted into the silkworm chrysalis slurry, drop in the hydrolysis kettle with meat grinder, the water that adds 2～4 times of quality, be heated to 65～70 ℃ after stirring, add 1～5% papain then, continue constant temperature and stirred 5～48 hours, carry out enzymolysis, after enzymolysis is finished, be warming up to 100～110 ℃, the enzyme that goes out sterilization 1～2 hour, after being cooled to room temperature, filter, filtrate changes knockout tower over to, leaves standstill, divide degrease, water places in the decompression concentration pan and concentrates, put cold, the impouring storage tank, spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder; 6. according to following each component and content (percentage by weight): oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; Amyloid auxiliary material 0～80% can be: flour, starch, bean powder, konjaku flour, black sesame powder, peanut powder; Excipient and thickener 0～10% can be: sodium carboxymethylcellulose, agar, gelatin, algin, pectin; Flavor enhancement 0～5% can be salt, sugar, honey, milk powder, albumen sugar, asccharin, monosodium glutamate, chickens' extract, pepper powder, zanthoxylum powder, chilli powder, green onion, ginger powder, garlic powder, peppermint; Vitamin 0～2% can be: vitamin A, Cobastab, vitamin C, vitamin D, vitamin E, vitamin K, citrin; Food coloring 0～2% can be: carrotene, caramel colorant, chocolate pigment, coffee pigment, orange pigment; Flavoring essence 0～2% can be: lychee flavor, flavoring apple essence, flavoring pineapple essence, strawberry essence, honey peach essence, cocoanut flavour, milk flavour, beef flavor, coffee aroma; Anticorrisive agent 0～1% can be: benzoate, antibacterial peptide, sorbate; The various forms of functional food such as dry powder that mixing can be made into tablet, powder, capsule, pill, electuary, biscuit, cake, instant noodles, congee paste or uses for mixing batter.This finished product taking dose is adult 0.1～100 gram/sky.
According to record such as " Chinese medicine voluminous dictionary " document, the fruit of glossy privet is returned liver, kidney channel, has effect nourishing liver and kidney, that the crow that makes eye bright sends out, is used for dizzy tinnitus, and soreness and weakness of waist and knees, poliosis, order are secretly not clear.Modern pharmacological research proves that the effective component oleanolic acid of the fruit of glossy privet has certain therapeutic action to experimental acute liver damage, can reduce the activity of glutamate pyruvate transaminase, the clinical treatment that is used for acute and chronic hepatitis, and curative effect is rather good.
Silkworm chrysalis is not only originated abundant, and has higher nutritive value and health care value.Silkworm chrysalis is that main body is formed with colloid albumen, and contains fat, glycogen, vitamin etc., and the protein content of bright pupa is up to 17%, and dry product is up to 50%, and fat content is 30%, and wherein unrighted acid accounts for 70%.The compound amino acid made from silkworm chrysalis is to feeding the effect that rising total serum protein and hemoglobin are arranged with the rat of no nitrogen basal feed; Experimental hepatitis rat there is the reduction serum glutamic pyruvic transminase, liver-protective effect.The glutamic-pyruvic transaminase value that experiment showed, the hepatitis rat of treatment after one month is recovered normally the report of also useful compound silkworm chrysalis treated powder chronic liver disease produce effects substantially.
The herbal component of this functional food comprises the fruit of glossy privet and silkworm chrysalis, adopts modern biochemical technology to extract its active ingredient, makes after mixing and add amyloid auxiliary material in proportion.The traditional handicraft of extracting oleanolic acid from the fruit of glossy privet adopts column chromatography, and this method cost is higher, and is loaded down with trivial details time-consuming, is not suitable for industrial production.The present invention adopts solvent extraction method to extract oleanolic acid, and is with low cost, easy to operation, products obtained therefrom purity height, (more than 90%).Prove that through pharmacology and toxicological experiment this functional food not only has the reduction transaminase, the effect of liver protecting, and safety non-toxic are taken without any side effects for a long time.This functional food production technology is simple, with low cost, taking convenience, effect are remarkable, has the value of applying.
Following introductory section zoopery result, experiment is undertaken by Disease Prevention Control Center, Hubei Prov.To carbon tetrachloride (CCl
4) cause the inhibitory action of mouse liver injury: get SPF level Kunming kind female mice (20 ± 2g) totally 50 are divided into five groups, 10 every group at random.This functional food is mixed with desired concn, and per os was irritated stomach and was given every day, and basic, normal, high dosage group dosage is respectively 0.15,0.30,0.45g/kg, normal control group and CCl
4Control group gives equivalent distilled water.In testing the 31st day with each treated animal fasting overnight 16 hours, CCl
4Control group and each dosage group give the CCl of 0.15% (v/v) through lumbar injection
4Oil solution 0.1ml/10g, the normal control group gives equivalent commercially available salad oil.Give CCl
4Put to death animal after 24 hours, get glutamic-pyruvic transaminase in the hematometry serum (ALT), glutamic-oxalacetic transaminease (AST).Experimental result shows, CCl
4Control group A LT and AST value obviously raise, each dosage group serum alt and AST value and CCl
4The apparent in view reduction of control group, difference has utmost point conspicuousness.
Table 1 is respectively organized mice serum ALT and AST measured value (the group dosage number of animals ALT AST of x ± s)
(g/kg) (n) (U/L) (U/L) normal control group 0 10 46.0 ± 5.5 162.4 ± 35.4CCl
4Control group 0 10 221.9 ± 85.8 514.8 ± 86.5 low dose group 0.15 10 154.4 ± 51.1
*303.9 ± 68.5
*Middle dosage group 0.30 10 110.3 ± 34.2
*273.0 ± 42.2
*High dose group 0.45 10 127.1 ± 22.6
*229.5 ± 72.1
*
*With CCl
4Control group is P＜0.01 relatively
The specific embodiment
Embodiment 1:
The dried fruit of glossy privet of 3kg is pulverized with pulverizer, extracted 2 times each 2 hours then with 70% alcohol reflux.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Sediment is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, adds hot water again and fully washs, and filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness running water, deionized water are cleaned with the fresh tussah chrysalis of 3kg, be twisted into the silkworm chrysalis slurry, drop in the hydrolysis kettle with meat grinder.Add the water of 4 times of quality, be heated to 65 ℃ after stirring, add 1% papain then, continue constant temperature and stirred 24 hours, carry out enzymolysis.After enzymolysis is finished, be warming up to 110 ℃, the enzyme that goes out sterilization 2 hours.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the decompression concentration pan and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.
By following pharmaceutical formulation: oleanolic acid 150g, silkworm chrysalis enzymolyzing dry powder 200g, starch 1000g, Sodium Benzoate 0.5g incapsulates by the 0.3g/ grain behind the mixing, packed products again behind the irradiation sterilization.Embodiment 2:
The dried fruit of glossy privet of 5kg is pulverized with pulverizer, extracted 3 times each 2 hours then with 75% alcohol reflux.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Sediment is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, adds hot water again and fully washs, and filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness running water, deionized water are cleaned with the fresh tussah chrysalis of 5kg, be twisted into the silkworm chrysalis slurry, drop in the hydrolysis kettle with meat grinder.Add the water of 4 times of quality, be heated to 70 ℃ after stirring, add 2% papain then, continue constant temperature and stirred 24 hours, carry out enzymolysis.After enzymolysis is finished, be warming up to 110 ℃, the enzyme that goes out sterilization 2h.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the decompression concentration pan and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.By following pharmaceutical formulation: oleanolic acid 200g, silkworm chrysalis enzymolyzing dry powder 300g, starch 500g, sodium carboxymethylcellulose 19.5g, Sodium Benzoate 0.5g is pressed into the liver-protecting tablet of 0.2g/ sheet behind the mixing.
Embodiment 3:
The dried fruit of glossy privet of 5kg is pulverized with pulverizer, extracted 3 times each 2 hours then with 75% alcohol reflux.Filter merging filtrate.Add H
2SO
4To the final mass percent concentration be 10%, stir, back flow reaction 3 hours reclaims ethanol.Sediment is washed till filtrate and becomes neutral with the NaOH liquid washing of an amount of pH8, adds hot water again and fully washs, and filters, and gets crude product.Activated carbon decolorizing, 95% ethyl alcohol recrystallization 2 times gets oleanolic acid.
After successively usefulness running water, deionized water are cleaned with the fresh tussah chrysalis of 5kg, be twisted into the silkworm chrysalis slurry, drop in the hydrolysis kettle with meat grinder.Add the water of 4 times of quality, be heated to 68 ℃ after stirring, add 2% papain then, continue constant temperature and stir 24h, carry out enzymolysis.After enzymolysis is finished, be warming up to 105 ℃, the enzyme that goes out sterilization 2h.After being cooled to room temperature, filter.Filtrate changes knockout tower over to, leaves standstill, and divides degrease.Water places in the decompression concentration pan and concentrates, put cold, the impouring storage tank.Spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder.Press following pharmaceutical formulation: oleanolic acid 300g, silkworm chrysalis enzymolyzing dry powder 300g, vitamin C 2.5g, Sodium Benzoate 0.5g makes electuary behind the mixing.
Claims (5)
1. the functional food with liver protection effect is characterized in that, the enzymolysis dry powder that oleanolic acid that extracts with the fruit of glossy privet and silkworm chrysalis make is as primary raw material, and its content is respectively: oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; And amyloid auxiliary material 0～80% in addition, excipient and thickener 0～10%, flavor enhancement 0～5%, vitamin 0～2%, food coloring 0～2%, anticorrisive agent 0～1%, the functional food of the liver protection effect of making.
2. according to the functional food of the described liver protection effect of claim 1, it is characterized in that described amyloid auxiliary material can be: flour, starch, bean powder, konjaku flour, black sesame powder, peanut powder; Described excipient and thickener can be: sodium carboxymethylcellulose, agar, gelatin, algin, pectin; Described flavor enhancement can be: salt, sugar, honey, milk powder, albumen sugar, asccharin, monosodium glutamate, chickens' extract, pepper powder, zanthoxylum powder, chilli powder, green onion, ginger powder, garlic powder, peppermint; Described vitamin can be: vitamin A, Cobastab, vitamin C, vitamin D, vitamin E, vitamin K, citrin; Described food coloring can be: carrotene, caramel colorant, chocolate pigment, coffee pigment, orange pigment; Described flavoring essence can be: lychee flavor, flavoring apple essence, flavoring pineapple essence, strawberry essence, honey peach essence, cocoanut flavour, milk flavour, beef flavor, coffee aroma; Described anticorrisive agent can be: benzoate, antibacterial peptide, sorbate.
3. the functional food of the described liver protection effect of claim 1 is characterized in that, the form of its functional food can be the dry powder that tablet, powder, capsule, pill, electuary, biscuit, cake, instant noodles, congee paste or confession mixing batter are used.
4. the preparation method of the functional food of a claim 1, this method comprise the following steps: 1. the dried fruit of glossy privet to be pulverized, and extract with alcohol reflux then, filter merging filtrate; 2. add H
2SO
4To the final mass percent concentration be 5～10%, stir, back flow reaction reclaims ethanol; 3. sediment is washed till filtrate and becomes neutral with an amount of NaOH solution washing, adds hot water and fully washs, and filters, and gets crude product, activated carbon decolorizing; 4. ethyl alcohol recrystallization is 1～2 time, gets oleanolic acid; 5. after fresh tussah chrysalis being cleaned, be twisted into the silkworm chrysalis slurry, drop in the hydrolysis kettle, add the water of 2～4 times of quality, stir and heat, add 1～5% papain then, continue constant temperature and stirred 5～48 hours, carry out enzymolysis, after enzymolysis is finished, be warming up to 100～110 ℃, the enzyme that goes out sterilization 1～2 hour, after being cooled to room temperature, filter, filtrate is left standstill, divide to separate degrease, the water decompression concentrates, and puts cold, the impouring storage tank, spray-dried, promptly get silkworm chrysalis enzymolyzing dry powder; 6. according to each component and percentage by weight: oleanolic acid 10～50%, silkworm chrysalis enzymolyzing dry powder 10～50%; And amyloid auxiliary material 0～80% in addition, excipient and thickener 0～10%, flavor enhancement 0～5%, vitamin 0～2%, food coloring 0～2%, anticorrisive agent 0～1% mixes, and makes various doses of shapes, product.
5. according to the method for claim 4, wherein the 1. described concentration of ethanol of step is 60～80%, needs refluxing extraction 2～3 times, each 1～3 hour; The 2. described back flow reaction of step needs 2～4 hours; 3. described its pH=8 of the NaOH aqueous solution of step～9; The 4. described concentration of alcohol of step is 90～99%; The temperature of the 5. described hydrolysis agitating heating of step is 65～70 ℃.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278740B (en) * | 2008-05-16 | 2010-12-29 | 孙平贵 | Health care silkworm chrysalis capable of improving immunity of human body |
| CN102715390A (en) * | 2012-05-03 | 2012-10-10 | 湖北炎帝农业科技股份有限公司 | Liver-protecting functional food and preparation method thereof |
| WO2013173972A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for growth in children |
| CN103535610A (en) * | 2013-09-18 | 2014-01-29 | 安徽省蚌埠市花乡食品有限责任公司 | Black peanut noodles capable of dispelling effects of alcohol and protecting liver |
| CN103734722A (en) * | 2013-12-27 | 2014-04-23 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method for silkworm pupa food, silkworm pupa food and application |
| CN105995593A (en) * | 2016-05-24 | 2016-10-12 | 广东汇香源生物科技股份有限公司 | Barbecuing essence and preparation method thereof |
-
2003
- 2003-06-05 CN CNB031281117A patent/CN1184902C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278740B (en) * | 2008-05-16 | 2010-12-29 | 孙平贵 | Health care silkworm chrysalis capable of improving immunity of human body |
| CN102715390A (en) * | 2012-05-03 | 2012-10-10 | 湖北炎帝农业科技股份有限公司 | Liver-protecting functional food and preparation method thereof |
| WO2013173972A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for growth in children |
| CN103535610A (en) * | 2013-09-18 | 2014-01-29 | 安徽省蚌埠市花乡食品有限责任公司 | Black peanut noodles capable of dispelling effects of alcohol and protecting liver |
| CN103734722A (en) * | 2013-12-27 | 2014-04-23 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method for silkworm pupa food, silkworm pupa food and application |
| CN103734722B (en) * | 2013-12-27 | 2015-05-13 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method for silkworm pupa food, silkworm pupa food and application |
| CN105995593A (en) * | 2016-05-24 | 2016-10-12 | 广东汇香源生物科技股份有限公司 | Barbecuing essence and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1184902C (en) | 2005-01-19 |
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