CN116918770B - Method for artificially propagating ladybug - Google Patents
Method for artificially propagating ladybug Download PDFInfo
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- CN116918770B CN116918770B CN202310682657.XA CN202310682657A CN116918770B CN 116918770 B CN116918770 B CN 116918770B CN 202310682657 A CN202310682657 A CN 202310682657A CN 116918770 B CN116918770 B CN 116918770B
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- 241000255749 Coccinellidae Species 0.000 title claims abstract description 158
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000001902 propagating effect Effects 0.000 title claims abstract description 17
- 238000009395 breeding Methods 0.000 claims abstract description 54
- 230000001488 breeding effect Effects 0.000 claims abstract description 51
- 241000173529 Aconitum napellus Species 0.000 claims abstract description 37
- 230000004083 survival effect Effects 0.000 claims abstract description 9
- 230000003020 moisturizing effect Effects 0.000 claims description 86
- 241000196324 Embryophyta Species 0.000 claims description 42
- 229940023019 aconite Drugs 0.000 claims description 36
- 241001083548 Anemone Species 0.000 claims description 32
- 230000032669 eclosion Effects 0.000 claims description 21
- 238000003780 insertion Methods 0.000 claims description 19
- 230000037431 insertion Effects 0.000 claims description 19
- 235000013601 eggs Nutrition 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 241000382353 Pupa Species 0.000 claims description 14
- 230000007246 mechanism Effects 0.000 claims description 13
- 230000019617 pupation Effects 0.000 claims description 13
- 230000003203 everyday effect Effects 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 6
- 230000002035 prolonged effect Effects 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 239000003550 marker Substances 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 230000000644 propagated effect Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 7
- 238000011161 development Methods 0.000 description 12
- 230000018109 developmental process Effects 0.000 description 12
- 230000002354 daily effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 241000270708 Testudinidae Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 235000021050 feed intake Nutrition 0.000 description 5
- 241001529602 Cassidinae Species 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 235000007823 Artemisia sp Nutrition 0.000 description 3
- 244000298939 Artemisia sp Species 0.000 description 3
- 241000218691 Cupressaceae Species 0.000 description 3
- 241000218180 Papaveraceae Species 0.000 description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000415040 Anemone rivularis Species 0.000 description 2
- 241001628605 Anemone tomentosa Species 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000227129 Aconitum Species 0.000 description 1
- 241001671653 Aconitum carmichaelii Species 0.000 description 1
- 241000512975 Epiverta chelonia Species 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for artificially propagating ladybug, which comprises the steps of breeding the ladybug by utilizing monkshood Aconiti lateralis Radix Praeparata in 3 to 10 months each year, breeding the ladybug by utilizing Anemone Anemone sp in 11 to 2 months each year, and repeating the propagation of the ladybug; the breeding method can ensure that the ladybug can always eat food in one year, improve the survival rate of the ladybug and ensure the population quantity.
Description
Technical Field
The invention belongs to the field of insect breeding and population propagation, and particularly relates to a method for artificially propagating ladybug.
Background
The Papaveris Epiverta chelonia is a species unique to China and belongs to Papaveraceae Cocciellidae, papaveraceae Epilochninae, papaveraceae Epivortini, papaveris Epivortini and Papaveris Epivorta. Ladybug mainly feeds on anemone Anemone tomentosa, prune Anemone rivularis and Artemisia sp. The national forestry agency of 8.1.2000 issued and implemented (No. 7) and listed as "beneficial for national protection or terrestrial wild animal directory with important economic and scientific research value".
At present, the research reports on the ladybug at home and abroad are few, and the main reasons are narrow habitat, difficult acquisition in the field, lack of living specimens for researching the primordial state, strict requirements on hosts, difficult propagation of indoor population and the like.
One of 40 crude drugs in China. Is processed product of radix Aconiti Kusnezoffii Aconitum carmichaelii Debx of Ranunculaceae. The aconite is mainly planted in Sichuan, southeast of Yunnan and southern of Shaanxi, etc. and is harvested in the late 6 months to 9 months. Fu Zi is pungent and sweet in flavor, is toxic due to strong heat, belongs to interior-warming herbs, has the effects of restoring yang and rescuing collapse, tonifying fire and supporting yang, dispelling cold and removing dampness, and is a first product of traditional Chinese medicine for restoring yang and rescuing collapse. Modern pharmacological researches have shown that aconite has the effects of strengthening heart, anti-inflammatory, analgesic and anti-aging. The traditional medicinal part of the aconite is the root, and the overground part (stem and leaf) is often discarded and not used, which not only causes continuous waste of traditional Chinese medicine resources, but also increases the burden on the ecological environment. If the overground part of the aconite can be reasonably utilized, for example, the overground part (stem and leaf) of the aconite is utilized to raise and propagate the ladybug, the materials can be fully utilized, the waste is changed into valuable, the waste of traditional Chinese medicine resources can be avoided, and win-win effect of medicine enterprises and farmers is realized.
Disclosure of Invention
The invention aims to provide a method for artificially propagating ladybug, which aims to solve the problems that living specimens of the original state of the ladybug are lack, indoor population is difficult to propagate and a corresponding breeding device is not available.
The invention adopts the following technical scheme: a method for artificially propagating Tortoise beetles comprises breeding Tortoise beetles with radix Aconiti lateralis Aconiti lateralis Radix Praeparata at 3-10 months each year, breeding Tortoise beetles with flos Trollii Anemone sp at 11-2 months each year, and repeating the propagation of Tortoise beetles.
Further, the ladybug is an Epivorta insect of the genus ladybug.
Further, the conditions for breeding the ladybug are as follows: the temperature is 16-26 ℃, the humidity is 60-80%, and the illumination is carried out for 12h at a ratio of 12h.
Further, the conditions for breeding the ladybug are as follows: the temperature is 20 ℃ and the humidity is 65% +/-5%.
Further, the method comprises the following steps:
step S1: collecting eggs of ladybug
Pairing the adult ladybug after eclosion, mating and spawning the adult ladybug, collecting eggs of the ladybug every day, placing the eggs on kitchen paper towels paved in a feeding device, marking spawning date and waiting for hatching;
step S2: breeding of larva of ladybug
Gently picking out the newly hatched larvae with a writing brush, receiving the newly hatched larvae into a feeding device filled with fresh aconite or anemone, periodically checking whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up, and immediately replacing the fresh leaves if the leaves are eaten completely or are dried up;
step S3: collecting ladybug pupa
After mature larva of ladybug pupates, if the position of the pupa is a blade, cutting the blade, placing the blade on kitchen paper towels paved in a feeding device, and marking the pupation date to wait for eclosion; if the pupa is in the feeding device and is not on the blade, a marker pen is used for circling at the position of pupation, the pupation date is marked, the eclosion is waited, and the eclosion is observed for 1 time every day;
step S4: breeding of adult ladybug
On the day of the emergence of the ladybug, putting fresh aconite or anemone into a feeding device, periodically checking whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up, and if the leaves are eaten completely or are dried up, immediately replacing the fresh leaves.
Further, the number of larvas of the ladybug raised in the raising device is 5-20, and the number of adults raised in the raising device is 2-20.
Further, in the breeding, the breeding device is used for preventing the ladybug from escaping and also used for moisturizing the host plant so that the ladybug can be propagated in a large quantity;
the feeding device comprises:
the shell is of a structure with an opening at the top and an upper layer and a lower layer, an insertion groove is arranged at the lower half part of the front side wall of the shell and at the position corresponding to the lower layer of the shell,
the raising ring plate is horizontally arranged and is positioned at the bottom of the upper layer of the shell, the outer edge of the raising ring plate contacts with the inner wall of the shell, the region corresponding to the bottom of the upper side of the shell in the annular hole is a raising region, the raising region is used for laying kitchen paper towels and raising ladybug on the kitchen paper towels, a plurality of inserting holes are obliquely arranged on the raising ring plate and penetrate through the side wall corresponding to the raising region, the inserting holes are used for obliquely inserting the root of the ladybug feeding plant downwards from top to bottom,
the moisturizing mechanism is positioned in the lower layer of the shell, is inserted into the lower layer of the shell through the insertion groove, stores water therein and is absorbed by the plant root passing through the insertion hole, so that the fresh-keeping and survival time of the plant is prolonged, and the ladybug is enabled to eat the plant leaves.
Further, the moisturizing mechanism includes:
the frame body is of a structure with an opening at the top and a hollow inside,
the moisturizing shell is vertically arranged and is of a cylindrical structure with openings at the upper and lower parts, the lower end of the moisturizing shell is fixedly connected with the bottom of the frame body, a plurality of shell through holes are horizontally penetrated through the side wall of the lower end of the moisturizing shell, the shell through holes are used for the water passing through the frame body,
the inner moisturizing shell is sleeved on the inner side of the outer moisturizing shell, is vertically arranged and is of a cylindrical structure with openings at the upper and lower parts, the lower end of the inner moisturizing shell is fixedly connected with the bottom of the frame body, a plurality of inner shell through holes are horizontally penetrated on the side wall of the lower end of the inner moisturizing shell and are used for being matched with the outer moisturizing shell to form a moisturizing cavity, the moisturizing cavity is arranged corresponding to the insertion holes, the inner shell through holes are used for moisture of the frame body to enter the moisturizing cavity,
the moisturizing strips are water-absorbing cotton and are filled in the moisturizing cavities of the moisturizing outer shell and the moisturizing inner shell and used for absorbing moisture in the inner cavity of the frame body through the moisturizing outer shell or the moisturizing inner shell and supplying the moisture to the roots of plants passing through the inserting holes.
Further, the upper end of the moisturizing strip is abutted against the bottom of the insertion hole.
Further, the left side and the right side of the frame body are fixedly connected with the otic placodes, the two otic placodes are composed of an upper otic placode and a lower otic placode which are symmetrical to each other, the position positions of the corresponding otic placodes in the lower layer of the shell are fixedly connected with the hanging plate, and the hanging plate is used for being inserted between the upper otic placode and the lower otic placode, so that the moisturizing mechanism is guided and connected.
The beneficial effects of the invention are as follows:
1. the breeding method can enable the plants which always have feeding in one year to feed, improves the survival rate of the ladybug and ensures the population quantity;
2. the humidity condition, the temperature condition and the illumination condition in the breeding method can ensure the necessary conditions for the normal growth and development of the ladybug, and a large number of ladybug populations can be obtained rapidly;
3. the breeding device ensures that eggs, larvae, pupae and adults of the ladybug do not directly contact water, ensures the hatching rate of the eggs of the ladybug, the eclosion rate of the pupae and the survival rate of the larvae and adults, ensures that the root of the eating plant of the ladybug is always kept with water supplement, prolongs the fresh-keeping and survival time of the eating plant, and reduces the frequency of replacing the eating plant;
4. according to the feeding device, the moisturizing outer shell and the moisturizing inner shell are arranged to form the moisturizing cavity, so that the moisturizing strips are positioned in the moisturizing cavity, and moisture is provided for roots of feeding plants.
Drawings
FIG. 1 is a schematic view of a feeding apparatus according to the present invention;
FIG. 2 is a schematic view of the structure of the moisturizing mechanism of the present disclosure;
FIG. 3 shows the daily feed intake (feeding area) of 1-instar larvae of the present invention;
FIG. 4 shows the daily feed intake (feeding area) of 2-year-old larvae of the present invention;
FIG. 5 shows the daily feed intake (feeding area) of 3-instar larvae of the present invention;
FIG. 6 shows the daily feed intake (feeding area) of 4-instar larvae of the present invention;
FIG. 7 shows the daily ration (feeding area) of adults according to the present invention.
Wherein: 1. a housing; 2. an insertion hole; 3. an insertion groove; 4. a moisturizing mechanism; 5. a frame; 6. a support ring; 7. a moisturizing shell; 8. a feeding area; 9. ear plates; 10. a moisture-retaining inner shell; 11. and (5) feeding the ring plate.
Detailed Description
The invention will be described in detail below with reference to the drawings and the detailed description.
According to the invention, the radix aconiti lateralis preparata and the anemone are alternately used in the breeding device to breed the ladybug, so that the ladybug can be propagated in a large amount indoors. Ladybug mainly feeds on anemone Anemone tomentosa, prune Anemone rivularis and Artemisia sp. However, the indoor feeding of the invention found that ladybug did not take on Artemisia sp. The ladybug takes the anemone, but after 4 months, the anemone in the market or in the field is basically harvested; meanwhile, as the anemone and the grass-jade plum belong to the same genus of the anemone, the growth cycle is similar, and therefore, the food necessary for the ladybug in the idle stage cannot be solved. Thus, if fed with a single host, the ladybug will die from the lack of food for some time. The growth period of the aconite is 2-10 months, the growth period of the aconite is staggered from the growth period of the anemone, and the aconite leaves are larger than the anemone leaves, so that the oviposition of the ladybug is facilitated, and the breeding is convenient. If the two host plants are alternately bred, the breeding of the ladybug population can be completely ensured.
The invention discloses a method for artificially propagating a tortoise ladybug, which comprises the steps of breeding the tortoise ladybug by using monkshood 3 months to 10 months each year, breeding the tortoise ladybug by using anemone each year 11 months to 2 months each year, and alternately using two plants to complete propagation of the tortoise ladybug repeatedly. Wherein the ladybug is Epivorta insect of the genus ladybug. The conditions for breeding the ladybug are as follows: the temperature is 16-26 ℃, the humidity is 60-80%, and the illumination is carried out for 12h at a ratio of 12h. Preferably, the conditions for breeding the ladybug are as follows: the temperature is 20 ℃ and the humidity is 65% +/-5%. When raising, the ladybug is placed in the raising device for raising, and the raising device not only can prevent the ladybug from escaping, but also is convenient for observing the growth and development conditions of the ladybug and moisturizing plants, so that the ladybug is bred in a large quantity.
Wherein, the breeding of the ladybug consists of the following steps:
step S1: collecting eggs of ladybug
Pairing the adult ladybug after eclosion, mating the adult ladybug with the adult ladybug, spawning, collecting eggs of the adult ladybug every day, placing the eggs on kitchen paper towels paved in a feeding device, marking spawning dates, and waiting for hatching.
Step S2: breeding of larva of ladybug
The newly hatched larvae are gently picked out by a writing brush and are connected into a feeding device filled with fresh aconite or anemone, whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up is checked periodically, and if the leaves are eaten completely or are dried up, the fresh leaves are replaced immediately.
It should be noted that when 4-instar larvae are bred, the relative humidity cannot be too high, and when the relative humidity reaches 85%, the pupation rate of the 4-instar larvae is obviously reduced, and the abdomen is easy to mould, so that the larvae die.
Step S3: collecting ladybug pupa
After mature larva of ladybug pupates, if the position of the pupa is a blade, cutting the blade, placing the blade on kitchen paper towels paved in a feeding device, and marking the pupation date to wait for eclosion; if the pupa is in the feeding device and is not on the blade, a marker pen is used for circling at the position of pupation, the pupation date is marked, the eclosion is waited, and the eclosion is observed for 1 time every day.
If the pupa is in the feeding device and is not on the blade, the pupa is not easy to move, the eclosion rate is affected by the movement, the eclosion adults are easy to be malformed, and only a marker pen is needed to draw circles at the position of the pupa, mark the pupa date and wait for eclosion.
Step S4: breeding of adult ladybug
On the day of the emergence of the ladybug, putting fresh aconite or anemone into a feeding device, periodically checking whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up, and if the leaves are eaten completely or are dried up, immediately replacing the fresh leaves. The relative humidity should not be too high when the adults are raised, and if the relative humidity exceeds 85%, the adults are prone to death.
The number of the larvas of the ladybug fed in the feeding device is 5-20, and the number of the adults fed in the feeding device is 2-20. The ladybug is not suitable for single-head feeding, the single-head feeding period can be obviously prolonged, and the ladybug is easy to death; the density of the ladybug mouth cannot be too high, and the ladybug mouth is easy to death and is not easy to observe.
As shown in fig. 1 and 2, the feeding apparatus includes: a housing 1, a feeding ring plate 11 and a moisturizing mechanism 4. The shell 1 has a top opening and an upper layer and a lower layer, and an insertion groove 3 is formed in the lower half part of the front side wall of the shell 1 and at a position corresponding to the lower layer of the shell 1.
The feeding ring plate 11 is horizontally arranged, the feeding ring plate 11 is positioned at the bottom of the upper layer of the shell 1, the outer edge of the feeding ring plate 11 is contacted with the inner wall of the shell 1, the region corresponding to the bottom of the upper side of the shell 1 in the annular hole of the feeding ring plate 11 is a feeding region 8, the feeding region 8 is used for laying kitchen tissues and feeding ladybug on the kitchen tissues, a plurality of inserting holes 2 are obliquely formed in the feeding ring plate 11 and on the side wall corresponding to the feeding region 8 in a penetrating mode, and the plurality of inserting holes 2 are used for obliquely downwards inserting the root of a plant taken by the ladybug from top to bottom.
Because eggs, larvae, pupae and adults of the ladybug cannot be directly contacted with water, once the eggs are contacted with water, the eggs are easy to mould, and the hatching rate is influenced; the pupa contacts with water to influence the pupation rate; larvae and adults are drowned out by direct contact with water. Therefore, the feeding plants are kept fresh by providing the insertion holes 2 without moisture directly entering the feeding area 8.
The upper side of casing 1 still is provided with the ventilation lid, and the ventilation lid is used for covering the upside at casing 1 to avoid the ladybug to escape, offered the vent on the ventilation lid, the vent department pastes has the gauze, and the gauze is used for ventilating in raising the in-process, opens the ventilation lid and can place the worm body and change the blade.
The moisturizing mechanism 4 is positioned in the lower layer of the shell 1, and is inserted into the lower layer of the shell 1 through the insertion groove 3, water is stored in the moisturizing mechanism 4 and is absorbed by the root of the plant passing through the insertion hole 2, so that the fresh keeping and survival time of the plant are prolonged, and the ladybug can eat fresh plant leaves.
The moisturizing mechanism 4 includes: a frame 5, a moisturizing outer shell 7, a moisturizing inner shell 10 and moisturizing strips.
The framework 5 is open-top, and inside hollow structure, and the shell 7 vertically sets up of moisturizing, and the shell 7 of moisturizing is the equal open-ended tubular structure from top to bottom, and the bottom fixed connection of shell 7 lower extreme and framework 5 of moisturizing, the level runs through on the shell 7 lower extreme lateral wall of moisturizing and has seted up a plurality of shell through holes, and the shell through hole is used for the moisture of framework 5 to pass through.
The inner shell 10 that moisturizes overlaps and establishes the shell 7 inboard that moisturizes, and the vertical setting of inner shell 10 that moisturizes, inner shell 10 that moisturizes is the tubular structure of upper and lower all open-ended, and the bottom fixed connection of inner shell 10 lower extreme and framework 5 moisturizes, has run through on the lateral wall of inner shell 10 lower extreme and has seted up a plurality of inner shell through holes horizontally, and inner shell 10 that moisturizes is used for mutually supporting with shell 7 that moisturizes and forms the cavity that moisturizes, and the cavity that moisturizes corresponds the patchhole 2 setting, and the inner shell through hole is used for the moisture of framework 5 to get into the cavity that moisturizes.
The moisturizing strips are absorbent cotton, are filled in the moisturizing cavities of the moisturizing outer shell 7 and the moisturizing inner shell 10, are used for absorbing moisture in the inner cavity of the frame body 5, pass through the moisturizing outer shell 7 or the moisturizing inner shell 10, and are supplied to the roots of plants passing through the inserting holes 2.
The upper end of the moisturizing strip is propped against the bottom of the insertion hole 2. If the upper end of the moisturizing strip is spaced apart from the bottom of the insertion hole 2, the length of inserting the root of the plant into the insertion hole 2 is required to be long, and thus the moisturizing effect on the plant is reduced, and although the freshness of the plant can be prolonged to some extent even though the root is not contacted with the moisturizing strip, the feeding requirement on the ladybug cannot be satisfied sufficiently.
The moisturizing mechanism 4 further comprises a supporting ring 6, the supporting ring 6 is vertically arranged, the supporting ring 6 is of a cylindrical structure with openings at the upper and lower sides, the lower end of the supporting ring 6 is fixedly connected with the bottom of the frame body 5, the moisturizing outer shell 7 is sleeved on the periphery of the supporting ring 6, the moisturizing inner shell 10 is sleeved between the moisturizing outer shell 7 and the supporting ring 6, and an area between the periphery of the supporting ring 6 and the frame body 5 is used for containing distilled water. The area of holding water of the frame body 5 can be reduced through the support ring 6, and then the depth of water is increased, so that more moisturizing strips are positioned below the water surface, and further, the root of the eating plant can absorb water more conveniently.
The inserting hole 2 is obliquely arranged from top to bottom, the root of a plant can be obliquely inserted through the obliquely arranged inserting hole 2, so that the inserting is easy to operate, in addition, the root of the plant can be guided to the upper end of the moisturizing strip, and the upper end of the moisturizing strip corresponds to the inserting hole 2, so that the root of the plant is contacted with the moisturizing strip after passing through the inserting hole 2, the freshness of the plant is ensured, and the feeding humidity requirement of the ladybug is fully met.
The left side and the right side of framework 5 are all fixedly connected with otic placode 9, and two otic placodes 9 comprise upper otic placode and the lower otic placode of mutual symmetry, in the lower floor of casing 1, correspond the equal fixedly connected link plate in position department of otic placode 9, the link plate is used for inserting between upper otic placode and the lower otic placode, and then plays direction and the effect of connection to moisturizing mechanism 4.
Example 1
8 months and 10 days in 2020, planting anemone, 11 months and 20 days in 2020, and planting radix Aconiti lateralis. When the aconite and anemone are planted, a layer of pine needles or cypress needles are covered on the planting machine, the thickness of the pine needles or cypress needles is about 3cm, the soil moisture conservation effect can be achieved, long-term observation shows that the pine needles or cypress needles are beneficial to the growth of the anemone and anemone, and meanwhile, the condition of soil hardening caused by uneven watering can be reduced. And (3) 2021, inoculating the ladybug with different insect states to the aconite for breeding, and transferring the ladybug to the anemone for breeding at 5 days of 2021 and 11.
Placing eggs of ladybug in a feeding device with kitchen paper towel at bottom, placing the feeding device in an environment with temperature of 16deg.C+ -1deg.C, relative humidity of 65deg.C+ -5% and photoperiod L:D=12h:12h, and placing distilled water in frame 5. After eggs are hatched, the larvae are removed and put into a new feeding device, then fresh aconite are put into the feeding device, and the roots of the aconite are inserted into the insertion holes 2, so that the roots of the leaves are contacted with the moisturizing strips. The same conditions are adopted, the aconite leaves are replaced regularly, and excrement is cleaned timely, so that the feeding environment is ensured to be clean.
The mature larva has less feeding and activity and low humidity, so the humidity in the feeding device is slightly reduced, and meanwhile, the artificial interference is reduced as much as possible when the mature larva is stationary, namely, the mature larva does not need to be manually moved/stirred so as not to influence pupation.
Because the molting time of the larva of the ladybug at the same age is not very regular, in order to ensure uniform age and size, the larva needs to be picked up after molting once every molting, namely entering the next age, and the larva needs to be transferred to a new feeding device,
continuing to feed, avoiding the problem of uneven age.
After pupation, the larvae are not shaken or moved as much as possible, otherwise, eclosion failure or adult malformation after eclosion can be caused.
Example 2
When the ladybug was bred, the temperature was kept at 20.+ -. 1 ℃ and the other conditions were the same as in example 1, and the conditions of the alternate breeding using two kinds of plants and the growth and development of the ladybug and whether the generation could be completed or not were observed in the breeding apparatus.
Example 3
When breeding the ladybug, the temperature was kept at 22.+ -. 1 ℃ and the other conditions were the same as in example 1, and the conditions of the alternate breeding using two kinds of plants and the growth and development of the ladybug and the completion of the generation in the breeding apparatus were observed.
Example 4
When breeding the ladybug, the temperature was kept at 25.+ -. 1 ℃ and the other conditions were the same as in example 1, and the conditions of the alternate breeding using two kinds of plants and the growth and development of the ladybug and whether the generation could be completed or not were observed in the breeding apparatus.
Example 5
When breeding the ladybug, the temperature was kept at 26.+ -. 1 ℃ and the other conditions were the same as in example 1, and the conditions of the alternate breeding using two kinds of plants and the growth and development of the ladybug and whether the generation could be completed or not were observed in the breeding apparatus.
Example 6
When the ladybug was bred, the temperature was kept at 28℃and the other conditions were the same as in example 1, and the conditions of the alternate breeding using two kinds of plants and the growth and development of the ladybug and whether the generation could be completed or not were observed in the breeding apparatus.
According to the results of examples 1-6, except for the temperature condition of example 6, two kinds of plants of aconite and anemone are alternately used for breeding the ladybug in the other examples, the generation of the ladybug can be completed in the breeding device of the invention, and the breeding of the ladybug population can be realized.
The results for examples 1-6 were counted as follows:
TABLE 1 morphological indices of larva of Tortoise
Note that the data in the table are mean ± standard deviation, and the same column of data is marked with different lowercase letters to indicate significant differences P < 0.05.
TABLE 2 development calendar of ladybug at different temperatures and different ages
Note that the data in the table are mean ± standard error, and the same column of data is marked with different lowercase letters to indicate that the difference is significantly P < 0.05.
TABLE 3 survival rates of ladybug larvae at different temperatures
TABLE 4 hatchability of eggs of ladybug and eclosion rate of pupae at different temperatures
TABLE 5 development calendar of single-head breeding of larva of ladybug
Tables 1-5 show that when the ladybug host plant anemone is deficient, the ladybug can be bred with the aconite, the growth cycle of the aconite is staggered from that of the anemone, and the aconite leaves are larger than those of the anemone, so that the ladybug host plant anemone is more suitable for oviposition of the ladybug, and the breeding is convenient. By alternately feeding the two plants, the breeding of the ladybug population can be completely ensured.
Tables 1-5 show that under the method of the invention, the generation of the ladybug can be completed completely by using the aconite to feed the ladybug, the morphological index has no obvious difference with the morphological index of the ladybug collected in the field, and the development calendar of the ladybug, the survival rate of larvae, the hatching rate of eggs, the eclosion rate of pupae and the like are basically similar to the field investigation result.
Meanwhile, tables 2 to 4 also show that the preferable breeding conditions of the ladybug are: the temperature is 20deg.C, the humidity is 65% + -5%, and the illumination time is 12 hr:12 hr. When raising, the ladybug is placed in the raising device for raising, and the raising device not only can prevent the ladybug from escaping, but also is convenient for observing the growth and development conditions of the ladybug and moisturizing plants, so that the ladybug is bred in a large quantity. Table 5 shows that when the ladybug is bred, the ladybug is not easy to be bred singly, and if the ladybug is bred singly, the development duration of the ladybug is obviously prolonged.
Figures 3-7 show, respectively, that under the method of the invention, the daily feed intake of the larvae of the ladybug, the ladybug at different ages, can be raised with the aconite, i.e. calculated as the area of the leaves of the feeding aconite, the trend of 'increasing before decreasing' is presented with the increase of the age of the day, and the feeding peak period is provided. In addition, when the ladybug larvae are about to molt, the ladybug larvae are all static and do not eat the aconite. The daily intake of adults is calculated as the area of the aconite leaves taken, and the total appearance of the aconite leaves is M-shaped with the increase of the day age. After eclosion, the adults do not take food immediately, and long-term observation shows that the adults stop taking food for 1-2 days after continuously taking food for a period of time, and continue taking food again.
The foregoing is only illustrative of the present invention and is not to be construed as limiting thereof, but rather as various modifications, equivalent arrangements, improvements, etc., within the spirit and principles of the present invention.
Claims (10)
1. A method for artificially propagating the ladybug is characterized in that the ladybug is bred by using aconite in 3 to 10 months each year, the ladybug is bred by using anemone in 11 to 2 months each year, and the propagation of the ladybug is completed repeatedly.
2. The method for artificially propagating ladybug in accordance with claim 1, wherein the ladybug is a ladybug genus insect.
3. The method for artificially propagating ladybug in accordance with claim 1 or 2, wherein the breeding conditions of the ladybug are: the temperature is 16-26 ℃, the humidity is 60-80%, and the illumination is 12: 12 h:12 h.
4. A method for artificially propagating ladybug in accordance with claim 3, wherein the breeding conditions of the ladybug are: the temperature is 20 ℃ and the humidity is 65% +/-5%.
5. The method for artificially propagating ladybug in accordance with claim 1, which is characterized by comprising the following steps:
step S1: collecting eggs of ladybug
Pairing the adult ladybug after eclosion, mating and spawning the adult ladybug, collecting eggs of the ladybug every day, placing the eggs on kitchen paper towels paved in a feeding device, marking spawning date and waiting for hatching;
step S2: breeding of larva of ladybug
Gently picking out the newly hatched larvae with a writing brush, receiving the newly hatched larvae into a feeding device filled with fresh aconite or anemone, periodically checking whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up, and immediately replacing the fresh leaves if the leaves are eaten completely or are dried up;
step S3: collecting ladybug pupa
After mature larva of ladybug pupates, if the position of the pupa is a blade, cutting the blade, placing the blade on kitchen paper towels paved in a feeding device, and marking the pupation date to wait for eclosion; if the pupa is in the feeding device and is not on the blade, a marker pen is used for circling at the position of pupation, the pupation date is marked, the eclosion is waited, and the eclosion is observed for 1 time every day;
step S4: breeding of adult ladybug
On the day of the emergence of the ladybug, putting fresh aconite or anemone into a feeding device, periodically checking whether the aconite or anemone leaves are eaten completely or whether the leaves are dried up, and if the leaves are eaten completely or are dried up, immediately replacing the fresh leaves.
6. The method for artificially propagating ladybug, according to claim 5, wherein the number of larvas of ladybug raised in the raising device is 5-20, and the number of adults raised in the raising device is 2-20.
7. The method for artificially propagating ladybug, according to claim 5 or 6, wherein the breeding device is used for preventing the ladybug from escaping during breeding, and is also used for moisturizing host plants so that the ladybug can be propagated in large quantity;
the feeding device includes:
the shell (1) is of a structure with an opening at the top and an upper layer and a lower layer, an insertion groove (3) is arranged at the lower half part of the front side wall of the shell (1) and at the position corresponding to the lower layer of the shell (1),
the feeding ring plate (11) is horizontally arranged and positioned at the bottom of the upper layer of the shell (1), the outer edge of the feeding ring plate is contacted with the inner wall of the shell (1), the region corresponding to the bottom of the upper side of the shell (1) in the annular hole is a feeding region (8), the feeding region (8) is used for laying kitchen tissues and feeding ladybug on the kitchen tissues, a plurality of inserting holes (2) are obliquely arranged on the feeding ring plate (11) and on the side wall corresponding to the feeding region (8) in a penetrating way, the plurality of inserting holes (2) are used for obliquely downwards inserting the root parts of ladybug feeding plants from top to bottom,
the moisturizing mechanism (4) is positioned in the lower layer of the shell (1), is inserted into the lower layer of the shell (1) through the insertion groove (3), stores water therein and is absorbed by the plant root passing through the insertion hole (2), so that the fresh-keeping and survival time of the plant is prolonged, and the ladybug is enabled to take the plant leaves.
8. The method for artificially propagating ladybug, according to claim 7, wherein the moisturizing means (4) comprises:
the frame body (5) has a structure with an open top and a hollow interior,
the moisturizing shell (7) is vertically arranged and is of a cylindrical structure with openings at the upper and lower sides, the lower end of the moisturizing shell is fixedly connected with the bottom of the frame body (5), a plurality of shell through holes are horizontally penetrated through the side wall of the lower end of the moisturizing shell, the shell through holes are used for the water passing through the frame body (5),
a moisturizing inner shell (10) sleeved on the inner side of the moisturizing outer shell (7) and vertically arranged, wherein the moisturizing inner shell is of a cylindrical structure with openings at the upper and lower sides, the lower end of the moisturizing inner shell is fixedly connected with the bottom of the frame body (5), a plurality of inner shell through holes are horizontally penetrated through the side wall of the lower end of the moisturizing inner shell and are used for being matched with the moisturizing outer shell (7) to form a moisturizing cavity, the moisturizing cavity is arranged corresponding to the inserting hole (2), the inner shell through holes are used for allowing moisture of the frame body (5) to enter the moisturizing cavity,
the moisturizing strips are water-absorbing cotton and are filled in the moisturizing cavities of the moisturizing outer shell (7) and the moisturizing inner shell (10) and used for absorbing moisture in the inner cavity of the frame body (5) through the moisturizing outer shell (7) or the moisturizing inner shell (10) and supplying the moisture to the roots of plants passing through the inserting holes (2).
9. The method for artificially propagating ladybug, according to claim 8, wherein the upper end of the moisturizing strip is abutted against the bottom of the insertion hole (2).
10. The method for artificially propagating ladybug, according to claim 9, wherein the left side and the right side of the frame body (5) are fixedly connected with ear plates (9), two ear plates (9) are composed of upper ear plates and lower ear plates which are symmetrical to each other, hanging plates are fixedly connected in the lower layer of the shell (1) at positions corresponding to the ear plates (9), and the hanging plates are used for being inserted between the upper ear plates and the lower ear plates so as to play a role in guiding and connecting the moisturizing mechanism (4).
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