CN116898741A - Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof - Google Patents

Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof Download PDF

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Publication number
CN116898741A
CN116898741A CN202311043519.3A CN202311043519A CN116898741A CN 116898741 A CN116898741 A CN 116898741A CN 202311043519 A CN202311043519 A CN 202311043519A CN 116898741 A CN116898741 A CN 116898741A
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gum
probiotics
lysozyme
hydrated silica
type
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郑嘉铭
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Guangzhou Pinhard Biotechnology Co ltd
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Guangzhou Pinhard Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/25—Silicon; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The application relates to the technical field of oral care, and particularly discloses gum repairing toothpaste containing lysozyme and probiotics and a preparation method thereof. A gum repairing toothpaste containing lysozyme and probiotics comprises 4% -7% of hydrated silica (MIC type), 3% -4.5% of hydrated silica (MIA type), 9% -12% of hydrated silica (165 type), 6% -8% of humectant, 1% -3% of surfactant, 2% -5% of stabilizer, 0.1% -0.5% of adhesive, 0.2% -0.6% of chelating agent, 0.1% -0.2% of preservative, 1% -3% of lysozyme, 2% -3% of lactobacillus and the balance of water. A preparation method of gum restoration toothpaste containing lysozyme and probiotics comprises the step of uniformly mixing all the components to obtain the gum restoration toothpaste containing lysozyme and probiotics. The gum repairing toothpaste has the effects of maintaining the microbial environment balance of the oral cavity, inhibiting halitosis and removing dental calculus.

Description

Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof
Technical Field
The application relates to the technical field of oral care, in particular to gum repairing toothpaste containing lysozyme and probiotics and a preparation method thereof.
Background
With the increasing medical level, people are increasingly focusing on oral health. It is well known that there is a large population of bacteria in the mouth, consisting of the oral environment inherent in the human body and the microorganisms therein, with an average of 400 or more bacteria per adult's mouth, in amounts of approximately 1000 hundred million. A wide variety of microorganisms co-inhabit in the oral environment, maintaining a dynamic equilibrium relationship with each other. Of course, among the numerous microorganisms in the oral cavity, there are also pathogenic microorganisms that may cause caries and periodontitis.
For a long time, it is considered that the purpose of treating periodontitis can be achieved by only destroying pathogenic microorganisms in the oral cavity. Although the effect of killing pathogenic microorganisms in the oral cavity has certain auxiliary treatment effect on oral diseases, the balance of the oral ecological system is inevitably destroyed while the pathogenic microorganisms in the oral cavity are killed, the drug resistance of the pathogenic microorganisms is enhanced, and the oral diseases are repeatedly repeated. The existing researches show that most pathogenic microorganisms in the oral cavity belong to conditional pathogenic bacteria, and oral diseases such as poor oral hygiene, pH change, immune system diseases and the like can be caused only under certain environmental changes, so that the researches also provide a new thought for the treatment of the oral diseases, and perhaps the purposes of relieving the treatment of the oral diseases or maintaining the oral health can be achieved from the aspect of maintaining the dynamic balance of the oral environment.
Most of toothpaste with functions of relieving and assisting in treating oral problems in the prior art has good antibacterial and bactericidal effects, and can inhibit the propagation and growth of pathogenic microorganisms in the oral cavity within a certain period of time so as to achieve the purpose of relieving oral diseases, but the toothpaste can inhibit the growth and propagation of other bacterial groups in the oral cavity, and the microbial groups in the oral cavity can be damaged after long-term use, so that the oral environment is unbalanced, and the oral diseases are further caused.
Disclosure of Invention
In order to maintain the dynamic balance of the oral environment, the application provides gum repairing toothpaste containing lysozyme and probiotics and a preparation method thereof. The application discovers that the combination of the lysozyme, the lactobacillus and the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) can obviously destroy the living environment of pathogenic microorganisms, relieve the discomfort of the oral cavity and inhibit halitosis, and the gum restoration toothpaste containing the lysozyme and the probiotics can consolidate the oral cavity environment and maintain the dynamic balance of the oral cavity environment after long-term use.
In a first aspect, the application provides a gum repairing toothpaste containing lysozyme and probiotics, which adopts the following technical scheme:
the gum restoration toothpaste containing lysozyme and probiotics comprises the following components in percentage by mass:
hydrated silica (MIC): 4% -7%;
hydrated silica (MIA type): 3% -4.5%;
hydrated silica (type 165): 9% -12%;
humectant: 6% -8%;
and (2) a surfactant: 1% -3%;
stabilizing agent: 2% -5%;
and (3) an adhesive: 0.1 to 0.5 percent;
chelating agent: 0.2 to 0.6 percent;
preservative: 0.1 to 0.2 percent;
lysozyme: 1% -3%;
lactic acid bacillus: 2% -3%;
the balance being water.
In the technical scheme, the lysozyme has the effects of inhibiting bacteria, diminishing inflammation and promoting wound healing, and the principle is that the lysozyme can destroy the cell wall of gram-positive bacteria to hydrolyze the gram-positive bacteria, and the lysozyme also has the effect of killing gram-negative bacteria under the synergistic effect of sIgA and complement, and can further improve the mucopolysaccharide metabolism of tissues, so that the purposes of diminishing inflammation and promoting wound healing are achieved.
Lactic acid bacteria are internationally recognized probiotics and have wide application in food processing, medical treatment and health and other aspects. The lactobacillus as gram positive bacteria can participate in the immune regulation mechanism of human body and regulate the dynamic balance of microorganisms in human body.
The hydrated silica (MIC type) is a medium-abrasive type silica, the hydrated silica (MIA type) is a medium-high abrasive type silica, and the hydrated silica (165 type) is a thickening type silica. The specific proportion of the mixed hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) can play a good role in cleaning dirt on the tooth surface.
The oral cavity and gum cleaning effect is achieved by the mutual cooperation of the lysozyme, the lactobacillus and the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the effects of diminishing inflammation and promoting wound healing of the lysozyme and the effect of regulating the dynamic balance of the oral cavity environment are achieved by the mutual cooperation of the lysozyme, the anti-inflammatory effect of the lysozyme and the effect of regulating the dynamic balance of the oral cavity environment by the lactobacillus, the hydrated silica (MIA type) and the hydrated silica (165 type), the good oral cavity and gum cleaning effect is achieved by the mutual cooperation of the lysozyme, the lactobacillus, the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the effects of obviously regulating the oral cavity flora, recovering the dynamic balance of the oral cavity environment and eliminating dental calculus and dental plaque are achieved by the combined action, the purposes of obviously inhibiting the growth and reproduction of pathogenic microorganisms in the oral cavity are achieved on the premise of avoiding enhancing the resistance of pathogenic microorganisms, the activity of periodontal cells is promoted, the effect of relieving oral cavity discomfort and the pain is achieved, and the oral cavity is inhibited by the effect of restoring oral cavity environment with the oral cavity and the oral cavity health care agent.
Preferably, the gum repairing toothpaste comprises 3.6-7% of whitening auxiliary agent, wherein the whitening auxiliary agent comprises a sage extract, a magnolia bark extract and a giant knotweed root extract.
According to the technical scheme, the whitening auxiliary agent consisting of the sage extract, the magnolia bark extract and the polygonum cuspidatum root extract is added into the gum restoration toothpaste containing lysozyme and probiotics, so that the effects of restoring the dynamic balance of the oral cavity environment and removing dental calculus and dental plaque can be achieved on the basis of the synergistic effect of the lysozyme, the lactobacillus, the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the adsorption capacity of the gum restoration toothpaste containing the lysozyme and the probiotics on metal ions and proteins is further improved, the adhesion and the propagation of pathogenic microorganisms on gums are inhibited, the dental plaque is obviously removed, the gum coloring is reduced, and the effects of consolidating the oral cavity environment, maintaining the dynamic balance of the oral cavity environment and whitening teeth can be achieved after long-term use.
Preferably, the salvia extract accounts for 0.5% -1% of the gum restoration toothpaste containing lysozyme and probiotics according to the mass percentage of the gum restoration toothpaste containing lysozyme and probiotics;
the magnolia bark extract accounts for 1% -2% of the gum restoration toothpaste containing lysozyme and probiotics;
the polygonum cuspidatum root extract accounts for 2% -3% of the gum restoration toothpaste containing lysozyme and probiotics.
According to the technical scheme, the quality of the sage extract, the magnolia bark extract and the giant knotweed rhizome extract in the gum restoration toothpaste containing lysozyme and probiotics is limited, so that the synergistic effect among the sage extract, the magnolia bark extract and the giant knotweed rhizome extract can be better, the adsorption capacity of the gum restoration toothpaste containing lysozyme and probiotics to metal ions and proteins is obviously promoted, the adhesion and propagation of pathogenic microorganisms to gums are inhibited, dental plaque is obviously removed, the coloration of the gums is reduced, and the effects of better consolidating the oral environment, maintaining the dynamic balance of the oral environment and whitening teeth can be achieved after long-term use.
Preferably, the mass ratio of the sage extract, the magnolia bark extract and the polygonum cuspidatum root extract is 1:2:2.
in the technical scheme, the mass ratio of the salvia officinalis extract to the magnolia bark extract to the polygonum cuspidatum root extract is 1:2:2, the synergistic effect among the sage extract, magnolia bark extract and polygonum cuspidatum root extract is best, and the excellent plaque removal and gum staining reduction effects can be achieved, so that the teeth are remarkably whitened.
Preferably, the surfactant is sodium lauryl sulfate.
According to the technical scheme, sodium laurylsulfate is selected as the surfactant, so that the gum restoration toothpaste containing lysozyme and probiotics can be stimulated to generate richer foam in the oral cavity, and the effective components can be fully contacted with the action sites, so that a better cleaning effect is achieved.
Preferably, the humectant is sorbitol.
In the technical scheme, the sorbitol is selected as the humectant, so that good fluidity of the gum restoration toothpaste containing lysozyme and probiotics can be maintained, and further, the sorbitol has a certain sweet taste, and better taste experience can be brought to the gum restoration toothpaste containing lysozyme and probiotics.
In a second aspect, the application provides a preparation method of gum restoration toothpaste containing lysozyme and probiotics, which adopts the following technical scheme:
a preparation method of gum restoration toothpaste containing lysozyme and probiotics comprises the following steps:
step 1: mixing water, humectant, chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), homogenizing for 5-8 min at 2000-2200 r/min;
step 2: adding adhesive, preservative and stabilizer, homogenizing for 5-8 min at 2000-2200 r/min;
step 3: adding surfactant, lysozyme and lactobacillus, homogenizing for 5-8 min at 2000-2200 r/min to obtain gum repairing toothpaste containing lysozyme and probiotics.
Preferably, the method comprises the following steps:
step 1: mixing water, humectant, chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), homogenizing for 5-8 min at 2000-2200 r/min;
step 2: adding adhesive, preservative and stabilizer, homogenizing for 5-8 min at 2000-2200 r/min;
step 3: adding surfactant, lysozyme, lactobacillus, magnolia bark extract, sage extract and polygonum cuspidatum root extract, homogenizing for 5-8 min at 2000-2200 r/min to obtain the gum restoration toothpaste containing lysozyme and probiotics.
According to the technical scheme, the components of the gum restoration toothpaste containing the lysozyme and the probiotics, which is prepared through the specific steps, are fully fused, so that the activities of the lysozyme and the lactobacillus are better reserved, and the gum restoration toothpaste containing the lysozyme and the probiotics can play a role in better consolidating the oral environment and maintaining the dynamic balance of the oral environment.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the oral cavity and gum cleaning effect is achieved by the mutual cooperation of the lysozyme, the lactobacillus and the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the effects of diminishing inflammation and promoting wound healing of the lysozyme and the effect of regulating the dynamic balance of the oral cavity environment are achieved by the mutual cooperation of the lysozyme, the anti-inflammatory effect of the lysozyme and the effect of regulating the dynamic balance of the oral cavity environment by the lactobacillus, the hydrated silica (MIA type) and the hydrated silica (165 type), the good oral cavity and gum cleaning effect is achieved by the mutual cooperation of the lysozyme, the lactobacillus, the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the effects of obviously regulating the oral cavity flora, recovering the dynamic balance of the oral cavity environment and eliminating dental calculus and dental plaque are achieved by the combined action, the purposes of obviously inhibiting the growth and reproduction of pathogenic microorganisms in the oral cavity are achieved on the premise of avoiding enhancing the resistance of pathogenic microorganisms, the activity of periodontal cells is promoted, the effect of relieving oral cavity discomfort and the pain is achieved, and the oral cavity is inhibited by the effect of restoring oral cavity environment with the oral cavity and the oral cavity health care agent.
2. According to the application, the whitening auxiliary agent composed of the sage extract, the magnolia bark extract and the polygonum cuspidatum root extract is added into the gum restoration toothpaste containing lysozyme and probiotics, so that the effects of restoring the dynamic balance of the oral cavity environment and removing dental calculus and dental plaque can be achieved on the basis of the synergistic effect of the lysozyme, the lactobacillus, the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type), the adsorption capacity of the gum restoration toothpaste containing lysozyme and probiotics on metal ions and proteins can be further improved, the adhesion and the reproduction of pathogenic microorganisms on the gum can be inhibited, the dental plaque can be remarkably removed, the coloration of the gum can be reduced, and the effects of consolidating the oral cavity environment, maintaining the dynamic balance of the oral cavity environment and whitening teeth can be achieved after long-term use.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present application, the present application will be further described with reference to the following specific examples.
Example 1
A gum restoration toothpaste containing lysozyme and probiotics, comprising 63.2kg of water, 4kg of hydrated silica (MIC type), 3kg of hydrated silica (MIA type), 12kg of hydrated silica (165 type), 8kg of humectant, 1kg of surfactant, 5kg of stabilizer, 0.1kg of adhesive, 0.6kg of chelating agent, 0.1kg of preservative, 1kg of lysozyme, 2kg of lactobacillus.
Among them, hydrated silica (MIC type), hydrated silica (MIA type), and hydrated silica (165 type) are purchased from Jin Sanjiang (Zhaoqing) silicon materials Co., ltd.
Wherein the humectant is sorbitol.
Wherein the surfactant is sodium lauryl sulfate.
Wherein the stabilizer is polyethylene glycol-8.
Wherein the adhesive is sodium carboxymethyl cellulose.
Wherein the chelating agent is tetrasodium pyrophosphate.
Wherein the preservative is methylparaben.
Among them, lysozyme was purchased from south Beijing Pang Bo Biotechnology Co.
Wherein, lactobacillus is purchased from Shandong Miao corporation's incubator Co., ltd, and the product name is lactobacillus powder SCP-1.
The preparation method of the gum repairing toothpaste containing lysozyme and probiotics comprises the following steps:
step 1: mixing water, humectant, chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), homogenizing at 2000r/min for 8min, and vacuum defoaming for 30min;
step 2: heating to 85deg.C, adding adhesive, antiseptic and stabilizer, homogenizing at 2000r/min for 8min, and vacuum defoaming until no foam is generated;
step 3: cooling to 40deg.C, adding surfactant, lysozyme, lactobacillus, cortex Magnolia officinalis bark extract, herba Salvia officinalis extract and rhizoma Polygoni Cuspidati extract, homogenizing at 2000r/min for 8min to obtain gum repairing toothpaste containing lysozyme and probiotic bacteria.
Example 2
Unlike example 1, a gum restoration toothpaste containing lysozyme and probiotics includes 61.9kg of water, 6kg of hydrated silica (MIC type), 4.5kg of hydrated silica (MIA type), 10kg of hydrated silica (165 type), 6kg of humectant, 3kg of surfactant, 2kg of stabilizer, 0.5kg of adhesive, 0.4kg of chelating agent, 0.2kg of preservative, 3kg of lysozyme, and 2.5kg of lactobacillus.
The preparation method of the gum repairing toothpaste containing lysozyme and probiotics comprises the following steps:
step 1: mixing water, humectant, chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), homogenizing at 2200r/min for 5min, and vacuum defoaming for 25min;
step 2: heating to 80 ℃, adding an adhesive, a preservative and a stabilizer, homogenizing for 5min at 2200r/min, and then vacuum defoaming until no foam exists;
step 3: cooling to 35deg.C, adding surfactant, lysozyme, lactobacillus, cortex Magnolia officinalis bark extract, herba Salvia officinalis extract and rhizoma Polygoni Cuspidati extract, homogenizing at 2200r/min for 5min to obtain gum repairing toothpaste containing lysozyme and probiotic bacteria.
Example 3
Unlike example 1, a gum restoration toothpaste containing lysozyme and probiotics includes 62.25kg of water, 7kg of hydrated silica (MIC type), 4kg of hydrated silica (MIA type), 9kg of hydrated silica (165 type), 7kg of humectant, 2kg of surfactant, 3kg of stabilizer, 0.4kg of adhesive, 0.2kg of chelating agent, 0.15kg of preservative, 2kg of lysozyme, 3kg of lactobacillus.
Example 4
Unlike example 2, a gum restoration toothpaste containing lysozyme and probiotics further comprises a whitening aid.
Wherein the whitening auxiliary agent comprises 1kg of sage extract, 2kg of magnolia bark extract and 2kg of giant knotweed root extract.
Example 5
Unlike example 4, the whitening aid comprises 0.5kg of the extract of Salvia officinalis, 1kg of the extract of Magnolia bark and 3kg of the extract of Polygonum cuspidatum root.
Example 6
Unlike example 4, the herb sage extract was replaced with rosemary leaf extract in equal amounts.
Example 7
Unlike example 4, the extract of magnolia bark replaced Cheng Guangguo licorice root extract by an equivalent amount of a gum restoration toothpaste containing lysozyme and probiotics.
Example 8
Unlike example 4, the gum restoration toothpaste containing lysozyme and probiotics was replaced with pseudo-ginseng extract in the same amount as the extract of Polygonum cuspidatum root.
Comparative example 1
Unlike example 2, the probiotic-containing gum restoration toothpaste was replaced with equal amounts of lysozyme as water.
Comparative example 2
Unlike example 2, the same amount of lactobacillus was replaced with water in the gum restoration toothpaste containing lysozyme.
Comparative example 3
Unlike example 2, the gum restoration toothpaste was replaced with water by the same amount of lysozyme and lactobacillus.
Comparative example 4
Unlike example 2, a gum restoration toothpaste containing lysozyme and probiotics was replaced with an equivalent amount of hydrated silica (MIC type) to hydrated silica (type 165).
Comparative example 5
Unlike example 2, a gum restoration toothpaste containing lysozyme and probiotics was replaced with an equivalent amount of hydrated silica (MIA type) to hydrated silica (165 type).
1. Bacteriostasis test
Antibacterial efficacy verification of gum restoration toothpaste
Referring to the antibacterial effect test method (suspension quantification method) of 7.3 antibacterial effect test method of daily chemical products in QB/T2738-2012 (evaluation method of antibacterial effect of daily chemical products), candida albicans (ATCC SC 5314), staphylococcus aureus (ATCC 6538), escherichia coli (ATCC 8739), porphyromonas gingivalis (ATCC 33277) and streptococcus mutans (ATCC UA 159) are used as test strains, the antibacterial rate of the gingiva restoration toothpaste after 5 times of dilution with purified water after 5 minutes of action time is tested, the test is repeated for 5 times, the average value is taken, and the test results are shown in Table 1.
Table 1:
in combination with example 2, example 4, comparative examples 1-3 and Table 1, the gum restoration toothpaste containing lysozyme and probiotics of the present application has good efficacy of inhibiting pathogenic microorganisms. In the special formula of the application, the lysozyme and the lactobacillus (SCP-1) have good synergistic effect, can jointly promote the antibacterial effect of the gum restoration toothpaste containing the lysozyme and the probiotics, and the lactobacillus (SCP-1) can well replace nutrients required by the growth of pathogenic microorganisms and can well cooperate with the inhibition effect of the lysozyme on the pathogenic microorganisms to achieve remarkable inhibition effect.
2. Test of Using Effect
Verification of dental effect of maintaining dynamic balance of oral environment and whitening of gum restoration toothpaste
Selection of test personnel: 455 subjects between 18 and 65 years of age met the following conditions: (1) physical well-being; (2) there are 12 natural anterior teeth: (3) Exogenous stain (Lobene stain index level between 2 and 3); (4) Gingivitis (gingival index (GI) between 1 and 2); (5) no additional trial or treatment was engaged during the trial; (6) No other toothpaste or mouthwash was used during the test; (7) endogenous staining of non-tetracycline teeth, fluorosis teeth, etc.; (8) smoking-free alcohol addiction; (9) Test researchers believe that other factors may affect the test results.
Test method 1: the test persons were randomly assigned to 13 groups, 35 persons in each group were brushed for 5min in the morning and evening, and Gingival Indexes (GI), gingival Bleeding Index (BI), lobene stain area index and Lobene stain extent index were evaluated at weeks 0, 4 and 12 of the test, and the results were averaged, and the change rate (%) of the Gingival Index (GI), the change rate (%) of the gingival Bleeding Index (BI), the change rate (%) of the Lobene stain area index and the change rate (%) of the Lobene stain extent index were calculated, and the change rate (%) = [ (average value of week 0 index-average value of week N index)/average value of week 0 index ]. 100%. The test results are shown in Table 2.
The Gum Index (GI) refers to the extent of gum lesions, and is divided into four classes, 0 to 3: level 0 indicates normal gums. Grade 1 indicates mild inflammation: slightly changed gingival color, slightly hyperemia, and no bleeding detected with a probe. Grade 2 represents moderate inflammation: gingival bleeding, edema, smooth and exploratory bleeding. Grade 3 indicates severe inflammation: gingival congestion, edema, ulcers, and spontaneous bleeding.
Gingival Bleeding Index (BI) refers to the severity of gingival bleeding, and is divided into four classes, 0 to 3: grade 0 indicates that there is no abnormal gum condition. Scale 1 indicates a slight change in gum color from a normal pale pink to a bright red. The level 2 indicates that a significant change in gum color, such as gum redness, occurs. Grade 3 indicates that the degree of gum lesions is very severe and bleeding occurs.
The Lobene stain index is a method for evaluating the color of teeth based on visual inspection, and is suitable for evaluating the effect of removing extrinsic stains (whitening) of teeth, and the area of the stain part and the degree of stains are evaluated in four grades of 0 to 3. The area grading scoring criteria are as follows: grade 0 indicates the presence of colorless plaques; scale 1 indicates that the stain does not exceed 1/3 of the tooth surface; grade 2 indicates that the stain does not exceed 2/3 of the tooth face; scale 3 indicates that the stain exceeds 2/3 of the tooth face. The degree ranking score criteria were as follows: grade 0 indicates the presence of colorless plaques; grade 1 represents light mottle (yellow or tan); grade 2 represents a moderate stain (medium brown); grade 3 represents heavy mottle (dark brown or black).
Test method 2: the recurrence rate of gingivitis was calculated during the test period (%) = (100% of gingivitis was repeated at least once after the gums recovered to normal within 12 weeks or the number of gingivitis was increased compared to the number of people before use/total number of people), and the test results are shown in table 3.
Test method 3: after the test person continuously used the gum restoration toothpaste for 12 weeks, satisfaction evaluation was made on the oral cavity odor improving effect, gum inflammation relieving effect and tooth whitening effect of the gum restoration toothpaste. The satisfaction evaluation score was 0 to 5 points, 0 points indicated very dissatisfaction, 1 points indicated dissatisfaction, 2 points indicated general, 3 points indicated comparatively satisfactory, 4 points indicated satisfactory, 5 points indicated very satisfactory, and the test results averaged as shown in table 3.
Table 2:
table 3:
in combination with examples 1 to 8, comparative examples 1 to 5 and tables 2 and 3, it is apparent that the gum restoration toothpastes containing lysozyme and probiotics of examples 1 to 3 and examples 6 to 8 have good effects of relieving oral discomfort and inhibiting halitosis, and the gum restoration toothpastes containing lysozyme and probiotics of examples 4 to 5 have good effects of relieving oral discomfort, inhibiting halitosis and whitening teeth.
In combination with example 2 and comparative examples 1 to 3, it can be seen that the gum restoration toothpaste containing lysozyme and probiotics, which is added with lysozyme and lactobacillus (SCP-1), has good effects of relieving oral discomfort and inhibiting halitosis. Specific analysis shows that lysozyme and lactobacillus (SCP-1) are matched for use, so that the dynamic balance of the oral cavity environment can be synergistically restored, and the lactobacillus (SCP-1) can destroy parasitic sites of substituted pathogenic microorganisms in the oral cavity under the action of the lysozyme, so that the growth of the pathogenic microorganisms in the oral cavity is obviously inhibited, the dynamic balance of the oral cavity environment is restored, and the effects of consolidating the oral cavity environment and maintaining the dynamic balance of the oral cavity environment can be achieved after the toothpaste is used for a long time.
In combination with example 2 and comparative examples 3 to 5, it can be seen that the gum restoration toothpaste containing lysozyme and probiotics, to which the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) were added, has a certain effect of removing dental plaque and dental plaque, and the gum restoration toothpaste containing lysozyme, lactobacillus (SCP-1) and hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) has more excellent effects of removing dental plaque and dental plaque, relieving oral discomfort and inhibiting halitosis; the concrete analysis shows that the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) can be well adhered to teeth, and meanwhile, the hydrated silica and the teeth have good friction force, so that the effect of cleaning dirt on the surfaces of the teeth is achieved, so that the dirt and dental plaque are reduced, further, the hydrated silica (MIC type), the hydrated silica (MIA type), the hydrated silica (165 type) and the lactobacillus (SCP-1) are matched with the lysozyme, the lactobacillus (SCP-1) and the lactobacillus (SCP-1) to replace parasitic sites of pathogenic microorganisms in cavities, the parasitic pathogenic microorganisms on the teeth are reduced, further, the dirt is an important parasitic site of the pathogenic microorganisms, the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) have good effect of cleaning dirt, namely, the important parasitic site of the pathogenic microorganisms is destroyed, and the growth of the pathogenic microorganisms is further inhibited, so that the oral cavity is excellent in that the oral cavity is well inhibited by adding the lysozyme, the lactobacillus (SCP-1) and the hydrated silica (MIA type) and the oral cavity is more excellent in effect of cleaning the bad breath, and the oral cavity is not easy to clean the dirt.
By combining the embodiment 2 and the embodiment 4-8, the whitening auxiliary agent consisting of the extract of the sage herb, the extract of the bark of magnolia officinalis and the extract of the polygonum cuspidatum root is added into the gum restoration toothpaste containing lysozyme and probiotics, so that the tooth whitening effect is remarkable. Specific analysis shows that the synergistic effect of the lysozyme, the lactobacillus (SCP-1), the hydrated silica (MIC type), the hydrated silica (MIA type) and the hydrated silica (165 type) can restore the dynamic balance of the oral cavity environment and the effect of removing dental calculus and dental plaque, and the addition of the sage extract, the magnolia bark extract and the giant knotweed root extract further improves the adsorption capacity of the gum restoration toothpaste containing the lysozyme and the probiotics on metal ions and proteins, inhibits the adhesion and propagation of pathogenic microorganisms on gums, obviously removes the dental plaque, reduces the gum staining, and can achieve the effects of strengthening the oral cavity environment, maintaining the dynamic balance of the oral cavity environment and whitening teeth after long-term use.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.

Claims (8)

1. The gum repairing toothpaste containing lysozyme and probiotics is characterized by comprising the following components in percentage by mass:
hydrated silica (MIC): 4% -7%;
hydrated silica (MIA type): 3% -4.5%;
hydrated silica (type 165): 9% -12%;
humectant: 6% -8%;
and (2) a surfactant: 1% -3%;
stabilizing agent: 2% -5%;
and (3) an adhesive: 0.1% -0.5%;
chelating agent: 0.2% -0.6%;
preservative: 0.1% -0.2%;
lysozyme: 1% -3%;
lactic acid bacillus: 2% -3%;
the balance being water.
2. The gum repairing toothpaste containing lysozyme and probiotics according to claim 1, further comprising 3.5% -6% of whitening auxiliary agent, wherein the whitening auxiliary agent comprises a sage extract, a magnolia bark extract and a polygonum cuspidatum root extract.
3. The gum restoration toothpaste containing lysozyme and probiotics according to claim 2, wherein the sage extract accounts for 0.5% -1% of the gum restoration toothpaste containing lysozyme and probiotics in terms of mass percent of the gum restoration toothpaste containing lysozyme and probiotics;
the magnolia bark extract accounts for 1% -2% of the gum restoration toothpaste containing lysozyme and probiotics;
the polygonum cuspidatum root extract accounts for 2% -3% of the gum restoration toothpaste containing lysozyme and probiotics.
4. The gum restoration toothpaste containing lysozyme and probiotics according to claim 1, wherein the mass ratio of the extract of sage, the extract of magnolia bark and the extract of polygonum cuspidatum root is 1:2:2.
5. a gum restoration toothpaste according to claim 1, wherein said surfactant is sodium lauryl sulfate.
6. A gum restoration toothpaste according to claim 1, wherein said humectant is sorbitol.
7. A method of preparing a gum restoration toothpaste containing lysozyme and probiotics as claimed in claim 1 comprising the steps of:
step 1: mixing water, a humectant, a chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), and homogenizing for 5-8 min at 2000-2200r/min;
step 2: adding an adhesive, a preservative and a stabilizer, and homogenizing for 5-8 min at 2000-2200r/min;
step 3: adding a surfactant, lysozyme and lactobacillus, and homogenizing for 5-8 min at 2000-2200 r/min to obtain the gum restoration toothpaste containing the lysozyme and the probiotics.
8. A method for preparing the gum restoration toothpaste containing lysozyme and probiotics according to any one of claims 2-4, comprising the following steps:
step 1: mixing water, a humectant, a chelating agent, hydrated silica (MIC type), hydrated silica (MIA type) and hydrated silica (165 type), and homogenizing for 5-8 min at 2000-2200r/min;
step 2: adding an adhesive, a preservative and a stabilizer, and homogenizing for 5-8 min at 2000-2200r/min;
step 3: adding a surfactant, lysozyme, lactobacillus, magnolia bark extract, salvia officinalis extract and polygonum cuspidatum root extract, and homogenizing for 5-8 min at 2000-2200r/min to obtain the gum restoration toothpaste containing lysozyme and probiotics.
CN202311043519.3A 2023-08-18 2023-08-18 Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof Pending CN116898741A (en)

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Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006117601A (en) * 2004-10-22 2006-05-11 Nippon Zettoc Co Ltd Composition for oral cavity
WO2006068011A1 (en) * 2004-12-22 2006-06-29 Lion Corporation Tooth whitening composition and tooth whitening set
CN102639189A (en) * 2009-12-04 2012-08-15 高露洁-棕榄公司 Oral compositions containing extracts of zingiber officinale and related methods
CN103083208A (en) * 2007-10-01 2013-05-08 高露洁-棕榄公司 Oral compositions containing botanical extracts
CN108578268A (en) * 2018-06-21 2018-09-28 广州康云生物科技有限公司 a kind of toothpaste containing lysozyme
CN109908072A (en) * 2019-04-09 2019-06-21 厦门蓝湾科技有限公司 A kind of marine polysaccharide toothpaste and preparation method thereof containing profitable probliotics
CN110237024A (en) * 2019-07-22 2019-09-17 佛山卡丝生物科技有限公司 A kind of whitening probiotics toothpaste and preparation method thereof
CN110279609A (en) * 2019-07-05 2019-09-27 广州康云生物科技有限公司 A kind of toothpaste containing lysozyme and plant extracts
CN110772475A (en) * 2019-12-10 2020-02-11 广州瑞誉化工科技有限公司 Probiotic toothpaste
CN111317708A (en) * 2020-02-28 2020-06-23 麦和(广州)实业有限公司 Soothing and refreshing toothpaste and preparation method thereof
CN111450038A (en) * 2020-06-12 2020-07-28 兰夫尼环保科技有限公司 Toothpaste for inhibiting oral helicobacter pylori
CN112438940A (en) * 2020-12-17 2021-03-05 宁波市朱氏贸易有限公司 Toothpaste capable of effectively inhibiting oral helicobacter pylori
CN113274309A (en) * 2021-05-11 2021-08-20 广州星际悦动股份有限公司 Oral care active particles, preparation method thereof and oral care product
CN113679632A (en) * 2021-09-01 2021-11-23 好易康生物科技(广州)有限公司 Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste
CN113797152A (en) * 2021-10-15 2021-12-17 兴宁市锐云贸易有限公司 Oral bacteriostatic toothpaste
CN114831910A (en) * 2022-04-06 2022-08-02 广州盛景家庭服务有限公司 Oral bacteriostatic paste for helicobacter pylori
CN115624499A (en) * 2022-11-17 2023-01-20 杭州三品良道商贸有限责任公司 Whitening and antiallergic toothpaste
CN115969743A (en) * 2022-08-16 2023-04-18 优齿乐(上海)健康用品有限公司 Toothpaste with fresh breath and preparation method thereof
CN116407465A (en) * 2023-06-10 2023-07-11 汕头市深泰新材料科技发展有限公司 Application of high molecular polymer in improving stability of toothpaste product

Patent Citations (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006117601A (en) * 2004-10-22 2006-05-11 Nippon Zettoc Co Ltd Composition for oral cavity
WO2006068011A1 (en) * 2004-12-22 2006-06-29 Lion Corporation Tooth whitening composition and tooth whitening set
CN103083208A (en) * 2007-10-01 2013-05-08 高露洁-棕榄公司 Oral compositions containing botanical extracts
CN102639189A (en) * 2009-12-04 2012-08-15 高露洁-棕榄公司 Oral compositions containing extracts of zingiber officinale and related methods
CN108578268A (en) * 2018-06-21 2018-09-28 广州康云生物科技有限公司 a kind of toothpaste containing lysozyme
CN109908072A (en) * 2019-04-09 2019-06-21 厦门蓝湾科技有限公司 A kind of marine polysaccharide toothpaste and preparation method thereof containing profitable probliotics
CN110279609A (en) * 2019-07-05 2019-09-27 广州康云生物科技有限公司 A kind of toothpaste containing lysozyme and plant extracts
CN110237024A (en) * 2019-07-22 2019-09-17 佛山卡丝生物科技有限公司 A kind of whitening probiotics toothpaste and preparation method thereof
CN110772475A (en) * 2019-12-10 2020-02-11 广州瑞誉化工科技有限公司 Probiotic toothpaste
CN111317708A (en) * 2020-02-28 2020-06-23 麦和(广州)实业有限公司 Soothing and refreshing toothpaste and preparation method thereof
CN111450038A (en) * 2020-06-12 2020-07-28 兰夫尼环保科技有限公司 Toothpaste for inhibiting oral helicobacter pylori
CN112438940A (en) * 2020-12-17 2021-03-05 宁波市朱氏贸易有限公司 Toothpaste capable of effectively inhibiting oral helicobacter pylori
CN113274309A (en) * 2021-05-11 2021-08-20 广州星际悦动股份有限公司 Oral care active particles, preparation method thereof and oral care product
CN113679632A (en) * 2021-09-01 2021-11-23 好易康生物科技(广州)有限公司 Application of antibacterial peptide compound DM80Bu20 in preparation of antibacterial toothpaste
WO2023029137A1 (en) * 2021-09-01 2023-03-09 好易康生物科技(广州)有限公司 Application of antibacterial peptide compound dm80bu20 in preparation of antibacterial toothpaste
CN113797152A (en) * 2021-10-15 2021-12-17 兴宁市锐云贸易有限公司 Oral bacteriostatic toothpaste
CN114831910A (en) * 2022-04-06 2022-08-02 广州盛景家庭服务有限公司 Oral bacteriostatic paste for helicobacter pylori
CN115969743A (en) * 2022-08-16 2023-04-18 优齿乐(上海)健康用品有限公司 Toothpaste with fresh breath and preparation method thereof
CN115624499A (en) * 2022-11-17 2023-01-20 杭州三品良道商贸有限责任公司 Whitening and antiallergic toothpaste
CN116407465A (en) * 2023-06-10 2023-07-11 汕头市深泰新材料科技发展有限公司 Application of high molecular polymer in improving stability of toothpaste product

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘合刚: "药用植物优质高效栽培技术", 中国医药科技出版社, pages: 464 *

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