CN108578268A - a kind of toothpaste containing lysozyme - Google Patents
a kind of toothpaste containing lysozyme Download PDFInfo
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- CN108578268A CN108578268A CN201810646166.9A CN201810646166A CN108578268A CN 108578268 A CN108578268 A CN 108578268A CN 201810646166 A CN201810646166 A CN 201810646166A CN 108578268 A CN108578268 A CN 108578268A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/27—Zinc; Compounds thereof
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- A61K8/442—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof substituted by amido group(s)
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Abstract
The invention discloses a kind of toothpaste containing lysozyme, including a kind of toothpaste additive, the toothpaste additive includes each component of following mass percent：Zinc compound 0.1~6%, lysozyme 0.01~3%, hydroxyapatite 0.1~20%.The toothpaste additive is by the way that zinc compound, lysozyme and hydroxyapatite to be used in combination, significant synergistic effect is played, the fungistatic effect of lysozyme can be enhanced, it can cover and reduce well again the metallic taste and bitterness sense that zinc salt is brought, the stability of its fungistatic effect can also be significantly improved simultaneously, it is persistently antibacterial, it reduces plaque, reduce bleeding gums.In addition to containing the compositions of additives in the toothpaste of the present invention, also contain moisturizer, polyethylene glycol, hydrated silica, adhesive, foaming agent, essence, chelating agent and sweetener, by the type and dosage that rationally compound each component, lysozyme activity can be effectively maintained, to achieve the purpose that lasting, Efficient antibacterial.
Description
Technical field
The present invention relates to lysozyme applied technical fields, and in particular, to a kind of toothpaste containing lysozyme.
Background technology
Oral problem mainly caused by bacterium, is arranged using effective gargle, toothpaste and oral disease therapeutic etc.
The quantity of pathogenic bacteria in oral cavity can be substantially reduced by applying, and improvement even is eliminated mouth disease.The oral cares such as mouthwash, toothpaste are produced
Why product can realize that these functions are mainly the active constituent or antipathogenic composition in product, these antipathogenic compositions are mostly medium-height grass
Medicine and chemical classes bacteriostatic agent, chemical classes bacteriostatic agent such as triclosan, Cetylpyridinium Chloride, chlorohexidene, antibiotic etc., commonly using containing this
The oral care product of a little ingredients be easy to cause oral bacteria ecological disturbance, or makes bacterial resistance occurred, cannot reach prevention
The effect of mouth disease causes other multiple oral diseases instead.
Lysozyme (Lysozyme) is a kind of non-specific material composition in saliva, can be suppressed growth of microorganism, with
Antibiotic or the comparison of other antibacterial substances, it is not in drug resistance that lysozyme, which is used for a long time,.Lysozyme, which is one kind, can be broken carefully
The obligate hydrolase of β-Isosorbide-5-Nitrae glycosidic bond in bacterium whole cell peptidoglycan between N-acetyl-glucosamine and -acetylmuramic acid, belongs to
Muramidase.Lysozyme can selectively solubilized target microorganism wall and make its inactivation, mammalian cell-free wall, because
This, lysozyme is not damaged to oral cavity tissue, but plays a protective role, and is added in oral care implement and inhibits oral cavity normal
See that pathogenic microorganisms has preferable promotion and application foreground.But since lysozyme sheet is as proteinaceous components, conventional mouth
Essential surfactant, foaming agent, binder, sweetener, chemical preservative etc. can different journeys in chamber nursing materials
Degree causes bacteriolyze enzyme denaturation, to cause its active reduction or loss.As Traditional blowing agents laruyl alcohol sodium sulfovinate with it is molten
When bacterium enzyme coordinates, white precipitate can be generated, lysozyme is caused to inactivate.Therefore, how effective protection lysozyme activity, enhancing bacteriolyze
Enzyme and sterilizing effect is that it is applied to one of the difficult point of oral care product.
In addition, the recurrent aphthous ulcer in oral mucosal disease has recurrent and self limiting, it is considered that pathogenic factor and people
Body immunity function is extremely related.In addition many researchs also indicate that zinc-deficiency is one of most important pathogenic factors of recurrent aphthous ulcer, because
This adds zinc salt in oral care product and is conducive to treat recurrent aphthous ulcer, still, zinc salt have metallic taste strongly,
Bitter taste and astringent sense, if the zinc salt containing high dose, sense of discomfort are especially strong in oral care product.
Invention content
The purpose of the invention is to overcome the above-mentioned deficiency of the prior art, a kind of toothpaste addition containing lysozyme is provided
Zinc compound and lysozyme are used in combination by agent, the present invention, on the one hand can improve the serum zinc concentration of oral cavity partial, zinc ion
By the interaction between inhibition bacterium glucose metabolism and bacteria cell wall, intraoral bacteria planting is reduced, and
It can reinforce lysozyme bactericidal and bacteriostatic effect in the presence of zinc ion；On the other hand, the two combined use can be covered and be dropped very well
The metallic taste and bitterness sense that low zinc salt is brought.In addition, by the way that hydroxyapatite is arrived zinc citrate according to specific proportioning compounding
In the composition of lysozyme, the stability of its effect can be significantly improved；The scheme combined by three, external source supplement oral cavity
Middle defence ingredient can inhibit the generation of bacterium and the formation of calculus dentalis, reduce plaque, reduce bleeding gums, can also adjust molten
Bacterium enzyme rate of release in the oral cavity extends the action time in mucous membrane of mouth or gingival sulcus, persistently antibacterial.
Another object of the present invention is to provide application of the above-mentioned toothpaste additive in preparing toothpaste.
Another object of the present invention is to provide a kind of toothpaste containing lysozyme.
To achieve the goals above, the present invention is achieved by following scheme：
A kind of toothpaste additive containing lysozyme, includes each component of following mass percent：Zinc compound 0.1~
6%, lysozyme 0.01~3%, hydroxyapatite 0.1~20%.
Biological lysozyme participates in the immune response of body as one of body nospecific immunity factor, to keep machine
Body physiological equilibrium.Lysozyme can also improve and enhance phagocytosis and the digestive function of macrophage, activated leukocyte phagocytic function, from
And enhance the immunity of body.Lysozyme also has the function of activating blood platelet, can improve tissue local blood circulation, enhance
Local defense function.Zinc compound and lysozyme are used in combination the present invention, on the one hand can improve the serum zinc of oral cavity partial
Concentration, zinc ion are reduced intraoral thin by inhibiting the interaction between bacterium glucose metabolism and bacteria cell wall
Bacterium is colonized, moreover, can reinforce lysozyme bactericidal and bacteriostatic effect in the presence of zinc ion；On the other hand, be used in combination can be with for the two
Cover and reduce very well the metallic taste and bitterness sense that zinc salt is brought.
In addition, the present invention according to specific proportioning by combining zinc compound, lysozyme and hydroxyapatite one
It rises, external source, which is supplemented, defends ingredient needed for ingredient and tooth in oral cavity, can inhibit the generation of bacterium and the formation of calculus dentalis, have and increase
Strong hard tooth tissue hardness, filling and repairing absence of tooth, blocks canaliculi dentales, reduces plaque, reduces bleeding gums strong tooth
Etc. functions.Hydroxyapatite is a kind of bioactive materials, identical as skeleton and tooth inorganic mineral constituents, can induce and repaiies
Renaturation dentinal permeability.The releasable calcium of hydroxyapatite, phosphorus plasma can participate in tissue metabolism, to be provided at dentine
Biological basis can repair dentine, alleviate sensitivity of tooth, and firm tooth promotes remineralization of teeth, reduces pre- anti-caries.In pH<
Lysozyme band cationic when 10.7, there is Ca on hydroxyapatite crystal surface2+And PO4 3-Anchor point passes through electrostatic interaction, lysozyme
Hydroxyapatite crystal surface can be adsorbed on.Hydroxyapatite itself and enamel surface affinity, can be adsorbed on tooth.
Hydroxyapatite is compounded with lysozyme, by the suction-operated of hydroxyapatite to lysozyme can be taken to enamel, exposure tooth sheet
Matter surface inhibits plaque to be formed；As environment pH changes, hydroxyapatite surface Ca2+And PO4 3-Ratio changes, absorption
Lysozyme is sustained out, and lysozyme adjustable so in the oral cavity in mucous membrane of mouth or gingival sulcus make by rate of release, extension
It is persistently antibacterial with the time.
Preferably, the toothpaste additive includes each component of following mass percent：Zinc compound 0.5~2.5%,
Lysozyme 0.05~2%, hydroxyapatite 0.5~20%.
It is highly preferred that the toothpaste additive includes each component of following mass percent：Zinc compound 1.0~
2.5%, lysozyme 0.1~1.5%, hydroxyapatite 1~15%.
The zinc compound, which includes but not limited to zinc citrate, zinc oxide, zinc chloride, zinc gluconate etc., to be provided
The compound of zinc ion, but one or more of which compounds.
Preferably, the zinc compound is any one of zinc citrate, zinc oxide, zinc chloride, zinc gluconate
Or it is a variety of.
The application of the toothpaste additive containing lysozyme in preparing toothpaste is also claimed in the present invention.
A kind of toothpaste containing lysozyme is also claimed in the present invention, and the toothpaste contains above-mentioned toothpaste additive.
Preferably, the toothpaste also contains each component of following mass percent：Moisturizer 10~70%, polyethylene glycol 1
~10%, hydrated silica 10~30%, adhesive 0.1~3%, foaming agent 1~6%, essence 0.1~3%, chelating agent 0.01~
3%, sweetener 0.01~1%.
It includes but not limited to Streptococcus mutans, gum porphin that the toothpaste, which can effectively inhibit the bacterium in oral cavity, the bacterium,
Quinoline monad, staphylococcus aureus, Escherichia coli.
Preferably, the foaming agent is fatty acyl sarcosines sodium, fatty acyl sodium glutamate, fatty amide propyl methyl sweet tea
Dish alkali, cocamidopropyl propyl amide methyl betaine any one or more of.The fatty acyl sarcosines sodium, fatty acyl glutamic acid
Sodium, fatty amide propyl methyl betaine and cocamidopropyl propyl amide methyl betaine are amphoteric surfactant, belong to mild
Surfactant has good foaminess, cleaning force, will not damage lysozyme activity.Therefore, bacteriolyze enzyme activity can not damaged
Preferable foam is also obtained while property, is conducive to the cleaning of tooth.
The foaming agent includes but not limited to fatty acyl sarcosines sodium, fatty acyl sodium glutamate, fatty amide propyl first
The amphoteric surfactantes such as base glycine betaine, cocamidopropyl propyl amide methyl betaine.
Preferably, the moisturizer is sorbierite, propylene glycol, glycerine any one or more of.
Preferably, the adhesive is sodium carboxymethylcellulose, carragheen, xanthans any one or more of.
Preferably, the chelating agent is pyrophosphate, phosphate, EDETATE SODIUM any one or more of.
Specific embodiment as one preferred, the toothpaste containing lysozyme contain each of following mass percent
Component：Sorbierite 40~60%, propylene glycol 0~5%, glycerine 0~5%, PEG4000~5%, hydrated silica 16~20%, carboxylic
Sodium carboxymethylcellulose pyce 0~1%, carragheen 0~1%, xanthans 0.3~0.5%, sodium cocoyl glutamate 0~1%, lauroyl
Sodium glutamate 0~2%, sodium lauroyl sarcosine 0~2%, cocamidopropyl propyl amide methyl betaine 1~2%, essence 1~3%,
Macrogol 6000 0~0.2%, Macrogol 4000 0~0.2%, polyethylene glycol 1500 0~0.2%, tetrasodium pyrophosphate 0~
2%, saccharin sodium 0.3~1%.
Compared with prior art, the invention has the advantages that：
(1) zinc compound and lysozyme are used in combination the present invention, produce significant synergistic effect, on the one hand can carry
The serum zinc concentration of high oral cavity partial, zinc ion is by inhibiting the phase interaction between bacterium glucose metabolism and bacteria cell wall
With reducing intraoral bacteria planting, and lysozyme bactericidal and bacteriostatic effect can be reinforced in the presence of zinc ion；On the other hand,
The two combined use can cover and reduce very well the metallic taste and bitterness sense that zinc salt is brought.
(2) compositions of additives provided by the invention containing lysozyme is by matching hydroxyapatite according to specific
In the composition for compounding zinc citrate and lysozyme, the stability of its effect can be significantly improved；The side combined by three
Case, external source supplement defend ingredient in oral cavity, can inhibit the generation of bacterium and the formation of calculus dentalis, reduce plaque, reduce gum
Bleeding can also adjust lysozyme rate of release in the oral cavity, extend the action time in mucous membrane of mouth or gingival sulcus, persistently antibacterial.
(3) in addition to containing the compositions of additives in toothpaste of the invention, also contain moisturizer, polyethylene glycol, hydration silicon
Stone, adhesive, foaming agent, essence, chelating agent, pigment and sweetener are such as selected by rationally compounding the type and dosage of each component
With amphoteric surfactant fatty acyl sarcosines sodium, fatty acyl sodium glutamate and fatty acyl with good foaminess, cleaning force
Amine hydroxypropyl methyl glycine betaine, also obtains preferable foam while not damaging lysozyme activity, be conducive to tooth cleaning and
Persistent high efficiency is antibacterial.
Specific implementation mode
The present invention is made with reference to specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
1, a kind of compositions of additives containing lysozyme, formula are that any one in formula 1~10 is formulated in table 1.
2, with reference to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effects, formula 1~10 in table 1 is measured
Composition to Streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, Escherichia coli inhibiting effect.And it is synchronous
The contrast test fungistatic effect of the lysozyme of corresponding content.
3, experimental result
(1) by the result of Tables 1 and 2 it is found that additive of the present invention composition can significantly improve the bacteriostasis of lysozyme.
In addition, also measured were the bacteriostasis rate (being shown in Table 2) when only adding zinc citrate, and zinc citrate and molten is added simultaneously
Lysozyme activity and bacteriostasis rate (being shown in Table 3) when bacterium enzyme.By table 2 and 3 result of table it is found that bacteriolyze can be enhanced after addition zinc citrate
Enzyme enzymatic activity, and the bacteriostasis of lysozyme can be significantly improved.
According to 1~10 proportioning of formula, the compositions of additives containing lysozyme is made, obtains mixture and tests its suppression at once
Bacterium rate (%).
The composition and fungistatic effect of the formula of table 1 1~10
Increased by being formulated the content it can be seen from 1~4 antibacterial result with hydroxyapatite in table 1, the suppression of composition
Bacterium rate has increase, but increases not notable.Compare the antibacterial of formula 1,5,6 as a result, with zinc citrate content increase, composition
Bacteriostasis rate increase is very notable.Compare the antibacterial of formula 7,8 as a result, in composition lysozyme content increase, bacteriostasis rate also increases
Add very significantly.Illustrate that zinc citrate and lysozyme are larger to composition Bacteriostatic Effect in composition.
Table 2 adds fungistatic effect when lysozyme, zinc citrate respectively
| Ingredient | It is formulated A | It is formulated B | It is formulated C | It is formulated D | It is formulated E | It is formulated F | It is formulated G | It is formulated H |
| Zinc citrate | / | / | / | / | / | 0.5 | 1.5 | 3.0 |
| Lysozyme | 0.1 | 0.5 | 1.0 | 2.0 | 3.0 | / | / | / |
| Deionized water | 99.9 | 99.5 | 99.0 | 98.0 | 97.0 | 99.5 | 98.5 | 97.0 |
| It is total | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
| Bacteriostasis rate (Streptococcus mutans) | 20.46 | 32.35 | 50.27 | 68.70 | 83.05 | 31.64 | 50.25 | 65.22 |
| Bacteriostasis rate (porphyromonas gingivalis) | 20.44 | 32.31 | 50.24 | 68.74 | 83.07 | 31.67 | 50.24 | 65.27 |
| Bacteriostasis rate (staphylococcus aureus) | 20.49 | 32.34 | 50.23 | 68.75 | 83.00 | 31.63 | 50.29 | 65.23 |
| Bacteriostasis rate (Escherichia coli) | 20.40 | 32.36 | 50.22 | 68.72 | 83.08 | 31.61 | 50.22 | 65.24 |
As shown in Table 2, the bacteriostasis of individual zinc citrate and lysozyme is not very strong, the dosage of the two
When higher respectively, bacteriostasis rate (%) just significantly improves.
After mixture mixes in table 3, bacteriostasis rate (%) is tested at once.
Table 3 adds fungistatic effect when lysozyme and zinc citrate simultaneously
| Ingredient | It is formulated I | It is formulated J | It is formulated K | It is formulated L | It is formulated M | It is formulated N | It is formulated O |
| Zinc citrate | 0.5 | 1.5 | 3.0 | 0.5 | 0.5 | 0.5 | 0.5 |
| Lysozyme | 1.0 | 1.0 | 1.0 | 0.1 | 0.5 | 2.0 | 3.0 |
| Deionized water | 98.5 | 97.5 | 96.0 | 99.4 | 99.0 | 97.5 | 96.5 |
| It is total | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
| Bacteriostasis rate (Streptococcus mutans) | 90.53 | 99.91 | 99.93 | 70.74 | 85.25 | 99.94 | 99.93 |
| Bacteriostasis rate (porphyromonas gingivalis) | 91.33 | 99.94 | 99.92 | 70.73 | 85.24 | 99.92 | 99.94 |
| Bacteriostasis rate (staphylococcus aureus) | 91.34 | 99.96 | 99.97 | 70.70 | 83.93 | 99.97 | 99.91 |
| Bacteriostasis rate (Escherichia coli) | 91.30 | 99.98 | 99.90 | 70.77 | 85.21 | 99.99 | 99.93 |
Contrast table 2 and table 3 as a result, understanding：After lysozyme and zinc citrate compound, the bacteriostasis rate of mixture significantly increases
By force, there is conspicuousness.This illustrates that lysozyme and zinc citrate while use produce synergistic effect, and the two compounding can more preferably inhibit
Bacterial growth or quickly kill bacterium.Zinc ion is combined with bacteria cell wall, softens cell wall, to lysozyme hydrolysis cell wall
Peptide glycan have catalytic action, accelerate its hydrolysis rate simultaneously lysozyme hydrolysis peptide glycan reaction rate improve, cell wall
Permeability is quickly opened, and zinc ion enters in cell, destroys bacterium internal structure, and the two coordinative role accelerates bacterial death.
(2) but in the presence of zinc ion, bacteriolyze enzyme denaturation may be made, with the extension of resting period, bacteriostasis rate is lasting
It reduces.The composition of contrived experiment test formulations I and formula J, and it additionally is added to the composition after hydroxyapatite, it stores
The bacteriostasis rate of different time, as a result as shown in table 4 and table 5.
After 4 lysozyme of table and zinc citrate mixing, fungistatic effect when different time is stored
After 5 lysozyme of table, zinc citrate and hydroxyapatite three mixing, fungistatic effect when different time is stored
The experimental result of contrast table 4 and table 5, it is known that individually addition zinc citrate and lysozyme, starting fungistatic effect is fine,
Extend at any time, bacteriostasis continuously decreases.After adding hydroxyapatite, antibacterial stable raising is antibacterial after placing 24 hours
Rate still has 99.95% or so.Independent zinc citrate coexists with lysozyme, and zinc ion gradually makes lysozyme become as time went on
Property, it loses activity.In the presence of hydroxyapatite, adsorbable lysozyme plays a protective role to lysozyme, lysozyme is avoided to become
Property, it stores for a long time, bacteriostasis rate will not reduce.Three compounds, by coordinating effect, enhance the fungistatic effect of lysozyme with it is steady
It is qualitative.
Embodiment 2
1, the specific formula of a kind of toothpaste containing lysozyme, toothpaste is shown in Table 6 application examples 1~10.Preparation process is as follows：
(1) water soluble ingredients such as polyethylene glycol, tetrasodium pyrophosphate, saccharin sodium, pigment add in container 1, and deionization is added
Water and stirring and dissolving；After having dissolved, sorbierite is added；
(2) propylene glycol or glycerine and adhesive are added in container 2, be dispersed with stirring uniformly, will be scattered after rubber powder,
It is added in container 1, is dispersed with stirring；
(3) to above-mentioned scattered system, hydrated silica and other powders are added, under vacuum 15 to 20 points of mixed at high speed
Clock；
(4) essence, foaming agent is added, is sufficiently mixed, disperses under vacuum, about 20 minutes；
(5) lysozyme is dissolved in deionized water, is added in above-mentioned scattered system, be sufficiently mixed, divide under vacuum
It dissipates, about 10 minutes.Up to toothpaste finished product.
2, it with reference to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effects, measures application examples 1~10 and corresponds to
Toothpaste to Streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, Escherichia coli inhibiting effect.By application examples 1~
After 10 45 DEG C of toothpaste high temperature are placed 3 months, antibacterial test is just done.And synchronism detection control 1, control 1 are laruyl alcohol except foaming agent
Outside sodium sulfovinate, the type and content of remaining component are identical as application examples 1.Measurement result is as shown in table 6.
6 application examples 1~10 of table, 1 formulation of tooth-paste of control and its bacteriostasis rate (%)
It is high to the bacteriostasis rate of different oral bacterias that 1~10 toothpaste of application examples is can be seen that according to the testing result of table 6
Up to 99% or more, illustrate that toothpaste of the present invention being capable of lasting, Efficient antibacterial；Compared with control 1, the bacteriostasis rate of 1 toothpaste of application examples is aobvious
It writes and improves, show the bacteriostatic activity that can effectively maintain lysozyme using amphoteric surfactant.
Embodiment 3
1, the specific formula of a kind of toothpaste containing lysozyme, toothpaste is shown in Table 7 application examples 11~15.Preparation process and implementation
Example 2 is identical.
2, it with reference to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effects, measures application examples 11~15 and corresponds to
Toothpaste to Streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, Escherichia coli inhibiting effect.By application examples 11
After~15 45 DEG C of toothpaste high temperature are placed 3 months, antibacterial test is just done.Measurement result is as shown in table 7.
7 application examples of table, 11~15 formulation of tooth-paste and its bacteriostasis rate (%)
| Application examples 11 | Application examples 12 | Application examples 13 | Application examples 14 | Application examples 15 | |
| Sorbierite | 40.00 | 40.00 | 40.00 | 40.00 | 60.00 |
| Propylene glycol | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Hydrated silica | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Hydroxyapatite | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Sodium carboxymethylcellulose | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Xanthans | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Sodium cocoyl glutamate | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Cocamidopropyl propyl amide methyl betaine | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Essence | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Zinc citrate | 0.50 | 0.50 | 0.50 | 1.00 | 0.50 |
| Lysozyme | 0.50 | 1.00 | 2.00 | 1.00 | 1.00 |
| Macrogol 4000 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
| Tetrasodium pyrophosphate | 2 | 2 | 2 | 2 | 2 |
| Saccharin sodium | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
| Deionized water | Surplus | Surplus | Surplus | Surplus | Surplus |
| It is total | 100% | 100% | 100% | 100% | 100% |
| Bacteriostasis rate (Streptococcus mutans) | 100.00 | 99.78 | 99.99 | 99.63 | 99.98 |
| Bacteriostasis rate (porphyromonas gingivalis) | 92.85 | 98.56 | 99.48 | 99.76 | 99.97 |
| Bacteriostasis rate (staphylococcus aureus) | 89.50 | 100.00 | 99.82 | 99.88 | 99.95 |
| Bacteriostasis rate (Escherichia coli) | 90.79 | 91.23 | 99.98 | 99.95 | 99.96 |
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all the present invention
All any modification, equivalent and improvement etc., should be included in the protection of the claims in the present invention made by within spirit and principle
Within the scope of.
Claims (10)
1. a kind of toothpaste additive containing lysozyme, which is characterized in that include each component of following mass percent：Containing zinc impregnation
Close object 0.1~6%, lysozyme 0.01~3%, hydroxyapatite 0.1~20%.
2. toothpaste additive according to claim 1, which is characterized in that the toothpaste additive includes following mass percent
Each component：Zinc compound 0.5~2.5%, lysozyme 0.05~2%, hydroxyapatite 0.5~20%.
3. toothpaste additive according to claim 1, which is characterized in that the zinc compound is zinc citrate, oxidation
Zinc, zinc chloride, zinc gluconate any one or more of.
4. application of any one of claims 1 to 3 toothpaste additive in preparing toothpaste.
5. a kind of toothpaste containing lysozyme, which is characterized in that contain any one of claims 1 to 3 toothpaste additive.
6. toothpaste according to claim 5, which is characterized in that also contain each component of following mass percent：Moisturizer 10
~70%, polyethylene glycol 1~10%, hydrated silica 10~30%, adhesive 0.1~3%, foaming agent 1~6%, essence 0.1~
3%, chelating agent 0.01~3%, sweetener 0.01~1%.
7. toothpaste according to claim 6, which is characterized in that the foaming agent is fatty acyl sarcosines sodium, fatty acyl paddy
Propylhomoserin sodium, fatty amide propyl methyl betaine, cocamidopropyl propyl amide methyl betaine any one or more of.
8. toothpaste according to claim 6, which is characterized in that the moisturizer is appointing in sorbierite, propylene glycol, glycerine
It is one or more.
9. toothpaste according to claim 6, which is characterized in that the adhesive is sodium carboxymethylcellulose, carragheen, Huang
Virgin rubber any one or more of.
10. toothpaste according to claim 6, which is characterized in that the chelating agent is pyrophosphate, phosphate, EDTA bis-
Sodium any one or more of.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109908025A (en) * | 2019-04-15 | 2019-06-21 | 广州康云生物科技有限公司 | Application of the glycine betaine in protection lysozyme activity |
| CN110251664A (en) * | 2019-07-05 | 2019-09-20 | 广州康云生物科技有限公司 | A kind of oral nursing liquid containing lysozyme and plant extracts |
| CN111701014A (en) * | 2020-08-12 | 2020-09-25 | 好易康生物科技(广州)有限公司 | Toothpaste for children and preparation method thereof |
| CN112220707A (en) * | 2020-11-10 | 2021-01-15 | 江苏雪豹日化有限公司 | Tooth sensitivity resisting biological lysozyme toothpaste composition |
| CN113813197A (en) * | 2021-10-29 | 2021-12-21 | 重庆登康口腔护理用品股份有限公司 | Composition for improving antibacterial activity and application thereof in oral products |
| CN116898741A (en) * | 2023-08-18 | 2023-10-20 | 广州品硬生物科技有限公司 | Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof |
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| CN109908025A (en) * | 2019-04-15 | 2019-06-21 | 广州康云生物科技有限公司 | Application of the glycine betaine in protection lysozyme activity |
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| CN113813197A (en) * | 2021-10-29 | 2021-12-21 | 重庆登康口腔护理用品股份有限公司 | Composition for improving antibacterial activity and application thereof in oral products |
| CN116898741A (en) * | 2023-08-18 | 2023-10-20 | 广州品硬生物科技有限公司 | Gum restoration toothpaste containing lysozyme and probiotics and preparation method thereof |
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