CN1167464C - Medicine for artificially inducing rockfish's sex reversion and preparing method and use - Google Patents

Abstract

The present invention provides a preparing method and the used of medicine for artificially inducing groupers' sex reversal. The medicine is a mixed hormone which is composed of methyltestosterone, testosterone, testosterone propionate and thyroxine; alga polysaccharide or alpha-starch is added to the mixed hormone to form strip-shaped medicine which is suitable to be embedded in fish bodies. The medicine prepared by the present invention is especially used as exogenous medicine which is embedded into female groupers' bodies to induce the groupers' sex reversal. An exogenous mixed hormone medicine strip body embedding method of the present invention is adopted, the time for female groupers' sex reversal is reduced within 80 days with stable and reliable effect through tests. The problem of sex recovery is not found till now.

Description

A kind of medicine of artificially inducing rockfish's sex reversion and preparation method

(1) technical field

The present invention relates to fish degeneration medicine and preparation thereof in the aquaculture.

(2) background technology

Cabrilla Li Shu Sushi section (Serranidae), cabrilla subfamily (Epinephelinae), Epinephelus (Epinephelus), the whole world is kind more than 400, China mainland is coastal to have write down 45 kinds of (Zhu Qian, 1998), the sex reversal process of ubiquity in its ontogeny " earlier female back is male ", the equal aging of male parent fish (more than general 6 ages); Large-scale kind (as: huge cabrilla) though female just ripe to 4 ages, and can ovulate; But more than 10 ages, just be transformed into functional milter during more than 30 kilogram of body weight.Reach the sexual inversion process more than 6 years, not only consume a large amount of fishes and use bait, and the existence of intersex, incured loss through delay the time of artificial propagation; Deng growth promoter when male spermary can be released essence, population quantity very rare and extremely difficulty catch.

The artificial propagation of cabrilla belongs to one of worldwide sea-farming difficult problem, and lithosporic piscinity control techniques is the key of artificial breeding.Cabrilla culturing economic benefit is huge, and coastal countries and area all spend substantial contribution to the research of tackling key problems of property control techniques, wish the artificial breeding industrialization; But property control success, and then develop into artificial breeding, do not see scientific research report and relevant patent.Singapore scholar Chao and Lim, Thailand scholar Tookwinas, Japan scholar basis is harvested, Philippine scholar Duray, China Taiwan's scholars leaf letter is sharp, and continent scholar Fang Yongqiang, Hong Wanshu etc. are respectively to the experimentation of huge cabrilla (Epinephelus tauvina), malaber reefcod (E.malabaricus), Fructus Citri tangerinae point cabrilla (E.suillus), green hata (E.awoara), the epinephelus akaara property done commentaries on classics controls such as (E.akaara) and artificial propagation aspect.Their method majority concentrates on the single bait feeding method of Methyl testosterone, and minority adopts the single injecting liquid drug method of Methyl testosterone, though these two kinds of methods are simple, but it is time-consuming, experiment is uncontrollable with the actual absorption of fish dosage, property commentaries on classics weak effect, and property commentaries on classics milter motility of sperm is not strong; And the injection of inactive bait feeding or medicine is after 5-7 month, and property commentaries on classics milter returns to the raun state again: the development of ovary is good, and spermary degenerates, and the restorative raun of part sex can also natural spawning; Even the temporary merit that changes into is arranged, as male parent, male and female also are difficult to pairing with this property commentaries on classics milter, even match the artificial fertilization once in a while, the prelarva that hatches also is difficult to survive, let alone the artificial breeding industrialization.Therefore, their research work major part is died young, and small part is still continuing research, but does not see breakthrough.In the later stage nineties, Taiwan is among the people by improving the parent fish rearing system, utilizes natural female, the male parent fish natural spawning fertilization of cage culture, and with germ cell or hatch fry and sell to the coastal area, Guangdong, but incubating oosperm planting percent less than 1%; Because of being subjected to male parent fish restricted number, fry quantity is very limited, and costs an arm and a leg.This approach can not fundamentally solve the industrialization of cabrilla artificial breeding and the large-scale farming demand to seedling.

(3) summary of the invention

The purpose of this invention is to provide a kind of medicine and preparation method to induce its sexual inversion in the cabrilla body that be used to be imbedded at, reaching can the shortening property commentaries on classics time, the reliable and stable effect of property commentaries on classics is to adapt to industrialization of cabrilla artificial breeding and the large-scale farming demand to seedling.

The pharmaceutical formulation component of artificially inducing rockfish's sex reversion provided by the present invention be through for many years screening and definite, the recipe ingredient of this medicine is as follows:

Methyl testosterone (17 α Methyltestosterone, 17 α MT) 11-30%;

Testosterone (Testosterone) 25-59%;

Testosterone Propionate (Testosterone Propionate) 5-20%;

Thyroxine (Thyronine) 3-19%.

The medicine of artificially inducing rockfish's sex reversion of the present invention is the exogenous medicine as the heeling-in method, generally make the form of medicated strip, can add 5%-10% Sargassum polysaccharides or 15-25% alphalise starch and make and be suitable for heeling-in in the intravital medicated strip shape of fish in described recipe ingredient, described medicated strip is best with cylindric.

Medicine of the present invention adopts following processing step preparation:

(1) with Methyl testosterone (17 α Methyltestosterone, 17 α MT), testosterone (Testosterone), Testosterone Propionate (Testosterone Propionate) and four kinds of hormones of thyroxine (Thyronine) in following recipe ingredient ratio uniform mixing:

Methyl testosterone (17 α Methyltestosterone, 17 α MT) 11-30%,

Testosterone (Testosterone) 25-60%,

Testosterone Propionate (Testosterone Propionate) 5-20%,

Thyroxine (Thyronine) 3-19%;

(2) in said mixture, add 5%-10% Sargassum polysaccharides or the abundant mix homogeneously of 15-25% alphalise starch, be modulated into sticking bulk, then it is squeezed into medicated strip with aseptic sea water bony fish normal saline;

(3) medicated strip after will being shaped is put in the sterilizing-drying culture dish, and is dry under aseptic condition.

Prepared medicine directly can be used or is stored in the brown wide mouthed bottle of evacuation, in the future standby.

The present invention prepared medicine be exclusively used in as the exogenous medicine that is imbedded in the female cabrilla body with inducing rockfish's sex reversion.Measure the long and body weight of body of every tail fish before the heeling-in earlier, and identify the age, extract the development condition that the live body product is examined under a microscope definitiveness cell and gonad with adopting oviduct, the dosage of selecting heeling-in according to age, body weight and gonad development situation is the external source hormotone medicated strip of 15～19mg/kg body weight.The position of heeling-in thinks that its scope is 8～15cm behind head gill cover upper limb, departs from 3.5～5cm place, dorsal line both sides in a back part both sides human body heeling-in best results after screening through strictness; When implanting medicated strip, with the parent fish folder parent fish of appropriateness anesthesia is fixed, fish body muscle of back is gone in an amount of medicated strip heeling-in, the wound base portion is coated with containing the antibiotic medicine binding in right amount, the parent fish that to implant medicated strip after finishing is put into tank, use double-deck sand filter seawater, the back of waiting to revive slowly moves into the reinforced cultivating pond.Through not only 100% survival of parent fish of above operation implantation medicated strip of the present invention, and property commentaries on classics effect is also very good.

Adopt external source hormotone medicated strip human body heeling-in method of the present invention,, the female lithosporic piscinity commentaries on classics time was shortened in 80 days, and more reliable and stable, do not find that so far sex restores problem through test.With this property commentaries on classics milter and natural raun pairing fertilization, hatched tail surplus the prelarva 210,000, breed 3cm commodity fry 30,000 tails.Experimental results show that no matter the heeling-in method is the stability of property commentaries on classics effect, or the high-tech content of operating process is all more first further than conventional bait feeding method and injection.

(4) specific embodiment

Embodiment one

When using Methyl testosterone (17 α Methyltestosterone, 17 α MT) 23%, testosterone (Testosterone) 56%, 5% 4 kind of hormone of Testosterone Propionate (Testosterone Propionate) 16% and thyroxine (Thyronine) mixes, add the abundant mix homogeneously of 8% Sargassum polysaccharides, be modulated into sticking bulk with aseptic sea water bony fish normal saline, firmly be crushed to cylindric medicated strip, experiment is anaesthetized with 0.5% urethanes with parent fish, the medicated strip of heeling-in is positioned at 14cm behind the head gill cover upper limb, departs from 4cm place, dorsal line both sides transdermal and punctures into muscle of back; The wound base portion uses double-deck sand filter seawater dipping bath experiment to use parent fish with after being mixed with the binding Ovum Gallus domesticus album glycerol antiinflammatory of 1% thymol and 0.01% nitrofural in right amount, and the back of waiting to revive slowly moves into the reinforced cultivating pond.Implant not only 100% survival of parent fish of hormotone medicated strip through above operation, and realize sexual inversion process in 80 days then, do not find the sex recovery so far by female change hero.

Embodiment two

When using Methyl testosterone (17 α Methyltestosterone, 17 α MT) 28%, testosterone (Testosterone) 40%, Testosterone Propionate (Testosterone Propionate) 17% and 15% 4 kind of hormone of thyroxine (Thyronine) mix, add the abundant mix homogeneously of 18% alphalise starch, be modulated into sticking bulk with aseptic sea water bony fish normal saline, firmly be crushed to cylindric medicated strip, experiment is anaesthetized with MS222 with parent fish, the medicated strip of heeling-in is positioned at 12cm behind the head gill cover upper limb, departs from 4cm place, dorsal line both sides transdermal and punctures into muscle of back; The wound base portion uses double-deck sand filter seawater dipping bath experiment to use parent fish with after being mixed with the binding Ovum Gallus domesticus album glycerol antiinflammatory of 1% thymol and 0.01% nitrofural in right amount, and the back of waiting to revive slowly moves into the reinforced cultivating pond.Implant not only 100% survival of parent fish of hormotone medicated strip through above operation, and realize sexual inversion process in 76 days then, do not find the sex recovery so far by female change hero.

Embodiment three

When using Methyl testosterone (17 α Methyltestosterone, 17 α MT) 27%, testosterone (Testosterone) 49%, 13% 4 kind of hormone of Testosterone Propionate (Testosterone Propionate) 11% and thyroxine (Thyronine) mixes, add the abundant mix homogeneously of 6% Sargassum polysaccharides, be modulated into sticking bulk with aseptic sea water bony fish normal saline, firmly be crushed to cylindric medicated strip, experiment is anaesthetized with 0.5% urethanes with parent fish, the medicated strip of heeling-in is positioned at 10cm behind the head gill cover upper limb, departs from 3.8cm place, dorsal line both sides transdermal and punctures into muscle of back; The wound base portion uses double-deck sand filter seawater dipping bath experiment to use parent fish with after being mixed with the binding Ovum Gallus domesticus album glycerol antiinflammatory of 1% thymol and 0.01% nitrofural in right amount, and the back of waiting to revive slowly moves into the reinforced cultivating pond.Implant not only 100% survival of parent fish of hormotone medicated strip through above operation, and realize sexual inversion process in 78 days then, do not find the sex recovery so far by female change hero.

Embodiment four

When using Methyl testosterone (17 α Methyltestosterone, 17 α MT) 17%, testosterone (Testosterone) 59%, Testosterone Propionate (Testosterone Propionate) 11% and 13% 4 kind of hormone of thyroxine (Thyronine) are mixed and made into cylindric medicated strip, experiment is anaesthetized with 0.5% urethanes with parent fish, the medicated strip of heeling-in is positioned at 10cm behind the head gill cover upper limb, departs from 4cm place, dorsal line both sides transdermal and punctures into muscle of back; The wound base portion uses double-deck sand filter seawater dipping bath experiment to use parent fish with after being mixed with the binding Ovum Gallus domesticus album glycerol antiinflammatory of 1% thymol and 0.01% nitrofural in right amount, and the back of waiting to revive slowly moves into the reinforced cultivating pond.Implant not only 100% survival of parent fish of hormotone medicated strip through above operation, and realize sexual inversion process in 80 days then, do not find the sex recovery so far by female change hero.

Claims (4)

1. the medicine of an artificially inducing rockfish's sex reversion is made medicated strip, it is characterized in that the recipe ingredient of medicine is as follows:

Sargassum Methyl testosterone 11-30%;

Testosterone 25-59%;

Testosterone Propionate 5-20%;

Thyroxine 3-19%.

2. the medicine of artificially inducing rockfish's sex reversion according to claim 1 is characterized in that by add 5%-10% Sargassum polysaccharides or the bonding medicated strip of making of 15-25% alphalise starch in described pharmaceutical formulation component.

3. the medicine of artificially inducing rockfish's sex reversion according to claim 3 is characterized in that described medicated strip is cylindric.

4. the preparation method of the medicine of the described artificially inducing rockfish's sex reversion of claim 1 is characterized in that:

(1) with Methyl testosterone, testosterone, Testosterone Propionate and four kinds of hormones of thyroxine in following recipe ingredient ratio uniform mixing:

Sargassum Methyl testosterone 11-30%,

Testosterone 25-60%,

Testosterone Propionate 5-20%,

Thyroxine 3-19%;

(2) in said mixture, add 5%-10% Sargassum polysaccharides or the abundant mix homogeneously of 15-25% alphalise starch, be modulated into sticking bulk, then it is squeezed into medicated strip with aseptic sea water bony fish normal saline;

(3) medicated strip after will being shaped is put in the sterilizing-drying culture dish, and is dry under aseptic condition.