CN106818547A - A kind of processing method for sticking up mouth Culter pseudo-milters - Google Patents

A kind of processing method for sticking up mouth Culter pseudo-milters Download PDF

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Publication number
CN106818547A
CN106818547A CN201611202227.XA CN201611202227A CN106818547A CN 106818547 A CN106818547 A CN 106818547A CN 201611202227 A CN201611202227 A CN 201611202227A CN 106818547 A CN106818547 A CN 106818547A
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China
Prior art keywords
pseudo
milters
feed
sticking
alpha
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CN201611202227.XA
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Chinese (zh)
Inventor
迟美丽
顾志敏
贾永义
刘士力
蒋文枰
程顺
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Zhejiang Institute of Freshwater Fisheries
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Zhejiang Institute of Freshwater Fisheries
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Priority to CN201611202227.XA priority Critical patent/CN106818547A/en
Publication of CN106818547A publication Critical patent/CN106818547A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/56Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/565Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
    • A61K31/568Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol substituted in positions 10 and 13 by a chain having at least one carbon atom, e.g. androstanes, e.g. testosterone
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/56Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/565Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
    • A61K31/568Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol substituted in positions 10 and 13 by a chain having at least one carbon atom, e.g. androstanes, e.g. testosterone
    • A61K31/569Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol substituted in positions 10 and 13 by a chain having at least one carbon atom, e.g. androstanes, e.g. testosterone substituted in position 17 alpha, e.g. ethisterone
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

The invention belongs to aquaculture field, a kind of processing method for sticking up mouth Culter pseudo-milters of optimization, including following process step are disclosed:(1)Gynogenesis after hatching in hatching cylinder sticks up mouth Culter juvenile fish;(2)Since the fries of 36 days after hatching into pond, testosterone propionate treatment;(3)17 α Methyltestosterones feed treatment.The present invention, using testosterone propionate and 17 α Methyltestosterones, is used in conjunction with for mouth Culter pseudo-milters are stuck up, and optimization processing mode, the method beneficial to the growth of mouth Culter pseudo-milters is stuck up is obtained, resulting sticks up mouth Culter pseudo-milters individuality greatly, growth is fast, and growth cycle is short, and economic worth is higher.

Description

A kind of processing method for sticking up mouth Culter pseudo-milters
Technical field
The invention belongs to aquaculture field, it is related to a kind of cultural method for sticking up mouth Culter, specifically a kind of optimization is stuck up The processing method of mouth Culter pseudo-milters.
Background technology
Stick up mouth Culter meat delicate in vain, it is delicious without raw meat, always it is considered as first-class economic fish.At present, cultivation is with sticking up mouth Culter Major part remains in the cultivation kind selected and remain using natural parent population or many generations, and not only its culture trait is not mined, and due to Has there are the different degrees of kinds such as slow-growing, meat quality decline in the reasons such as Juvenile stage is improper, inbreeding, culture trait Matter decay.
Artificial gynogenesis induction is that fish are quickly fixed maternal character and carry out the important technical of sexual control, can Accelerate the breeding process and efficiency of fish especially generation cycle fish more long.Patent No. CN201310546582.9 discloses one Plant and stick up mouth Culter gynogenesis abductive approach, carp sperm is selected as activation, through ultraviolet fire extinguishing sperm genetic substance, cold shock Suppress the release of ovum second polar body, be successfully established and stuck up the heterologous thelykaryon abductive approach of mouth Culter, and obtained a large amount of first generation thelykaryons Development fish and multiple second generation gynogenesis fishes familys, important technology branch is provided subsequently to stick up mouth Culter seed selections and complete female breeding Support.
But at present, the treatment for sticking up mouth Culter pseudo-milters obtained to the above method never has more preferable scheme, cultivates Pseudo-milter with compared with other fishes that batch is cultivated, individual substantially less than normal, development is slow, largely limiting after entirely The nursery production work of raun.
The content of the invention
It is an object of the invention to provide a kind of processing method for sticking up mouth Culter pseudo-milters, targetedly enter to sticking up mouth Culter pseudo-milters Row treatment, by optimization, what is obtained sticks up the growth of mouth Culter pseudo-milters soon, and cycle is short is individual big.
In order to achieve the above object, the present invention is adopted the following technical scheme that:A kind of processing method for sticking up mouth Culter pseudo-milters, It is jointly processed by sticking up mouth Culter pseudo-milter fries using testosterone propionate and 17 Alpha-Methyl stosterones.
At present, disclosure is processed pseudo-milter using 17 Alpha-Methyl stosterones and stosterone, but to sticking up mouth Culter, this Two kinds are used alone or are used in conjunction with, and effect is not all reached.Using the testosterone propionate and 17 Alpha-Methyl stosterones of the application, Process sticking up mouth Culter pseudo-milters, the growing state for sticking up mouth Culter pseudo-milters can be substantially improved, growth is fast, it is individual big.
Preferably, above-mentioned processing method includes following process step:(1)Gynogenesis after hatching in hatching cylinder Stick up mouth Culter juvenile fish;(2)Since the fries of 3-6 days after hatching into pond, testosterone propionate treatment;(3)17 Alpha-Methyl stosterones Feed treatment.
Preferably, step(2)The concrete operations of middle testosterone propionate treatment are:Testosterone propionate powder is dissolved in alcohol laggard Row full pool spilling head, testosterone propionate concentration is 0.8-1.2g/L.
Preferably, step(2)In the concentration splashed be every mu of pond 0.35-0.5g testosterone propionate.
Preferably, step(2)Middle testosterone propionate splashed the time for 12-16 days.
Preferably, step(3)In, the treatment that feeds of 17 Alpha-Methyl stosterones is to use to be processed through 17 Alpha-Methyl stosterones Feed fed.
Preferably, the concrete operations of 17 Alpha-Methyl stosterones treatment feed are:According to dissolving 30- in every kilogram of alcohol The 17 Alpha-Methyl stosterones of 150mg, 17 Alpha-Methyl stosterones are dissolved into alcohol, and are raised according to 500mL alcohol sprinkling 5kg The principle of material is uniformly sprayed onto on feed, is then placed on feed and alcohol is vapored away at shady and cool ventilation, is put into freezer and is frozen.
Preferably, 17 Alpha-Methyl stosterones fed process time for 100-110 days.
Preferably, 17 Alpha-Methyl stosterones feed treatment and was divided into and be at least divided into for five phases and feed, the respectively first phase, second Phase, the third phase, the fourth phase, the fifth phase, the 17 Alpha-Methyl testosterone concentrations for sprinkling feed of the first phase to the fifth phase according to The amplitude of at least 10mg/ kg gradually rises.
Further preferably, 17 Alpha-Methyl testosterone concentrations of sprinkling feed used for the first phase to the fifth phase are respectively 30- 35 mg/kg、60-65 mg/kg、90-100 mg/kg、120-130mg/kg、140-150 mg/kg。
Preferably, the first phase to the second phase, the elevation amplitude of the 17 Alpha-Methyl testosterone concentrations of the second phase to the third phase The elevation amplitude of the 17 Alpha-Methyl testosterone concentrations of the fourth phase and the fourth phase to four or five phases is played more than the 3rd.
Preferably, being the 2-4% of fish weight through the feed quantity of 17 Alpha-Methyl stosterones treatment feed.
By implementing above-mentioned technical proposal, the present invention is directed to sticks up mouth Culter pseudo-milters, using testosterone propionate and 17 Alpha-Methyl testis Ball ketone, is used in conjunction with, and optimization processing mode, obtains the method beneficial to the growth of mouth Culter pseudo-milters is stuck up, and resulting sticks up mouth Culter puppets Milter is individual big, and growth is fast, and growth cycle is short, and economic worth is higher.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1:
A kind of processing method for sticking up mouth Culter pseudo-milters, including following process step:(1)Gynogenesis after hatching in hatching cylinder Stick up mouth Culter juvenile fish;(2)Since the fries of 6 days after hatching into pond, testosterone propionate powder is dissolved in after alcohol carry out it is complete Pond is splashed, and testosterone propionate concentration is 1.2g/L;Every mu of pond 0.5g testosterone propionate, splashes 16 days;(3)Using through 17 Alpha-Methyl testis The feed of ball ketone treatment is fed, and point five phases feed 100 days.Feed containing 17 Alpha-Methyl stosterones prepares concrete operations: 17 Alpha-Methyl stosterones are dissolved into alcohol, the 17 Alpha-Methyl stosterones that each phase is used to process feed are different, and by the One phase to the fifth phase is increased with the amplitude of at least 10mg/ kg.In the present embodiment, the 30mg/ kg that the first phase is used, by 30mg 17 Alpha-Methyl stosterones be dissolved into 1 kg alcohol;The second phase uses 100mg/ kg, the 4th using 60mg/ kg, the third phase Phase uses 140mg/ kg using 120mg/ kg, the fifth phase.17 Alpha-Methyl stosterones of above-mentioned each concentration, according to 500mL alcohol The principle for spraying 5kg feeds is uniformly sprayed onto on feed, is then placed on feed and alcohol is vapored away at shady and cool ventilation, is put into cold Frozen in storehouse.
Embodiment 2:
A kind of processing method for sticking up mouth Culter pseudo-milters, including following process step:(1)Gynogenesis after hatching in hatching cylinder Stick up mouth Culter juvenile fish;(2)Since the fries of 4 days after hatching into pond, testosterone propionate powder is dissolved in after alcohol carry out it is complete Pond is splashed, and testosterone propionate concentration is 1.0g/L;Every mu of pond 0.42g testosterone propionate, splashes 16 days;(3)Using through 17 Alpha-Methyls The feed of stosterone treatment is fed, and point five phases feed 105 days, 20 days each issue of preceding fourth phases.Feeding containing 17 Alpha-Methyl stosterones Material prepares concrete operations:17 Alpha-Methyl stosterones are dissolved into alcohol, each phase is used to process 17 Alpha-Methyl testis of feed Ball ketone is different.In the present embodiment, the 17 Alpha-Methyl stosterones of 30mg are dissolved into 1 kg wine by the 32mg/ kg that the first phase is used In essence;The second phase is used using 95mg/ kg, the fourth phase using 62mg/ kg, the third phase using 120mg/ kg, the fifth phase 145mg/ kg.17 Alpha-Methyl stosterones of above-mentioned each concentration, uniformly spray according to the principle of 500mL alcohol sprinkling 5kg feeds Onto feed, then feed is placed on alcohol is vapored away at shady and cool ventilation, be put into freezer and freeze.(4)Feed without 17 α-first Base stosterone Jing treasured feed.
Embodiment 3:
A kind of processing method for sticking up mouth Culter pseudo-milters, including following process step:(1)Gynogenesis after hatching in hatching cylinder Stick up mouth Culter juvenile fish;(2)Since the fries of 3 days after hatching into pond, testosterone propionate powder is dissolved in after alcohol carry out it is complete Pond is splashed, and testosterone propionate concentration is 0.8g/L;Every mu of pond 0.5g testosterone propionate, splashes 15 days;(3)Using through 17 Alpha-Methyl testis The feed of ball ketone treatment is fed, and point five phases feed 110 days, 20 days each issue of preceding fourth phases.Feed containing 17 Alpha-Methyl stosterones Preparing concrete operations is:17 Alpha-Methyl stosterones are dissolved into alcohol, each phase is used to process 17 Alpha-Methyl testis of feed Ketone is different.In the present embodiment, the 17 Alpha-Methyl stosterones of 35mg are dissolved into 1 kg alcohol by the 35mg/ kg that the first phase is used In;The second phase is used using 100mg/ kg, the fourth phase using 65mg/ kg, the third phase using 125mg/ kg, the fifth phase 150mg/ kg.17 Alpha-Methyl stosterones of above-mentioned each concentration, uniformly spray according to the principle of 500mL alcohol sprinkling 5kg feeds Onto feed, then feed is placed on alcohol is vapored away at shady and cool ventilation, be put into freezer and freeze.(4)Feed without 17 α-first Base stosterone Jing treasured feed.

Claims (10)

1. a kind of processing method for sticking up mouth Culter pseudo-milters, it is characterised in that common using testosterone propionate and 17 Alpha-Methyl stosterones Mouth Culter pseudo-milter fries are stuck up in treatment.
2. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 1, it is characterised in that including following treatment step Suddenly:(1)Gynogenesis after hatching in hatching cylinder sticks up mouth Culter juvenile fish;(2)The fry of 3-6 days opens into pond from after hatching Begin, testosterone propionate treatment;(3)17 Alpha-Methyl stosterones feed treatment.
3. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 2, it is characterised in that step(2)Middle propionic acid testis Ketone treatment concrete operations be:Testosterone propionate powder is dissolved in carries out full pool spilling head after alcohol, testosterone propionate concentration is 0.8- 1.2g/L。
4. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 3, it is characterised in that step(2)In splash Concentration is every mu of pond 0.35-0.5g testosterone propionate.
5. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 3, it is characterised in that step(2)Middle propionic acid testis Ketone splashed the time for 12-16 days.
6. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 2, it is characterised in that step(3)In, 17 α-first The treatment that feeds of base stosterone is fed using the feed processed through 17 Alpha-Methyl stosterones.
7. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 6, it is characterised in that at 17 Alpha-Methyl stosterones Managing the concrete operations of feed is:17 Alpha-Methyl stosterones are dissolved into alcohol according to 30-140mg/L, and according to 500mL wine The principle of essence sprinkling 5kg feeds is uniformly sprayed onto on feed, is then placed on feed and alcohol is vapored away at shady and cool ventilation, is put into Frozen in freezer.
8. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 7, it is characterised in that 17 Alpha-Methyl stosterones are thrown It is 100-110 days to feed process time.
9. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 7, it is characterised in that 17 Alpha-Methyl stosterones are thrown Feed treatment and be divided into and be at least divided into for five phases and feed, the 17 Alpha-Methyl testosterone concentrations for sprinkling feed of the first phase to the fifth phase by Edge up height.
10. a kind of processing method for sticking up mouth Culter pseudo-milters according to claim 6, it is characterised in that through 17 Alpha-Methyl testis The feed quantity of ketone treatment feed is the 2-4% of fish weight.
CN201611202227.XA 2016-12-23 2016-12-23 A kind of processing method for sticking up mouth Culter pseudo-milters Pending CN106818547A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377703A (en) * 2001-03-29 2002-11-06 中国科学院南海海洋研究所 Medicine for artificially inducing rockfish's sex reversion and preparing method and use
CN101224238A (en) * 2006-12-13 2008-07-23 天津市弗兰德医药科技发展有限公司 External methyl tes tosterone preparation
CN102578001A (en) * 2011-01-05 2012-07-18 广西玉林市牧源渔牧有限公司 Unisexual fry raising technique for walking catfish
CN102988986A (en) * 2012-11-02 2013-03-27 浙江万里学院 Male trait inducer and induction method for pelodiscus sinensis in embryonic periods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377703A (en) * 2001-03-29 2002-11-06 中国科学院南海海洋研究所 Medicine for artificially inducing rockfish's sex reversion and preparing method and use
CN101224238A (en) * 2006-12-13 2008-07-23 天津市弗兰德医药科技发展有限公司 External methyl tes tosterone preparation
CN102578001A (en) * 2011-01-05 2012-07-18 广西玉林市牧源渔牧有限公司 Unisexual fry raising technique for walking catfish
CN102988986A (en) * 2012-11-02 2013-03-27 浙江万里学院 Male trait inducer and induction method for pelodiscus sinensis in embryonic periods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
龚文杰 等: "丙酸睾酮对网箱养殖条件下黄鳝生长的影响", 《湖北农业科学》 *

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Application publication date: 20170613

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