TWI645780B - Soaking treatment with ethinyl estradiol to induce Eurasian koi fish Female method - Google Patents

Abstract

The invention discloses a method for inducing femaleization of Eurasian koi fish, which comprises immersing Eurasian koi fish with a fish age of 1 to 3 dph in an acetylene female having a concentration ranging from 142 to 200 ppm. The diol lasts from 1 to 3 minutes.

Description

Soaking treatment with ethinyl estradiol to induce Eurasian koi fish Female method

The present invention relates to a method for inducing femaleization of Eurasian tilapia, which comprises immersing Eurasian koi fish of fish age 1 to 3 dph in acetylene having a concentration ranging from 142 to 200 ppm. Estradiol lasts 1 to 3 minutes.

The tilapia is a group of freshwater fish belonging to the Cichlidae family. It has the advantages of rapid growth, strong reproductive ability, good adaptability and omnivorousness, and has become the first aquaculture fish in the world. Commonly farmed species include Oreochromis niloticus [also known as Nile tilapia] and Oreochromis aureus [also known as Oria wu] Tilapia aurea] and so on.

When males and females are mixed and cultured, Wu Guoyu consumes energy by self-propagation, which makes the growth rate slow. Furthermore, the increase in the number of offspring produced by breeding leads to the breeding pond. The density of breeding is too high, which in turn causes growth to stagnate. Therefore, mono-sex culture has been widely adopted to solve the above problems. In addition, in the case of parthenogenesis, the growth rate and body weight of male fish are It is now twice as high as that of females. Therefore, how to obtain male Wu Guoyu has become a goal in this field. Although the male Eurasian squid is smaller in size, the male Eurasian osculature (ZZ ♂) is used to mate with the female Nile squid (XX ♀) [ie, interspecific hybridization ( Interspecific hybridization]], then a larger male all-male tilapia hybrids (XZ ♂). Therefore, for aquaculture producers, there is a need to develop a method to improve the offspring of male Eurasian koi fish.

At present, attempts have been made to induce the femaleization of Eurasian koi fish by feeding with estrogen (estrogen), and by using the femaleized Eurasian koi fish (ZZ △ ♀) and male Ori Yawu Guoyu (ZZ ♂) is mated to increase the male rate of the offspring. For example, in Melard (1995), Aquaculture , 130:25-34, Melard will be formulated with ethinyl estradiol in 95% ethanol [also known as 17α-ethynyl estradiol (17α-ethynylestradiol). )] Sprayed in the feed to obtain a feed containing 100, 150 and 200 mg/kg of ethinyl estradiol, respectively. Then, the three kinds of feeds were fed to the Olia squid fry for 40 days. The experimental results showed that 100, 150 and 200 mg/kg of ethinyl estradiol were used to induce the femaleization of Eurasian mullet, and the female traits of Eurasian mullet were 94% and 93%, respectively. 98%. Melard further mated the obtained female Aussie squid with the male Eurasian squid, and the males obtained from the progeny ranged from 68% to 100%.

Since the use of estrogen to feed to induce females takes a long time and has poor palatability and uneven feeding of estrogen, it is difficult to ensure that all of the Eurasian squid fry have sufficient estrogen intake. Therefore, if we can develop an induction method that is convenient and fast to operate and has a stable female rate, it will be achieved by us.

Summary of invention

Accordingly, the present invention provides a method for inducing femaleization of Eurasian koi fish, which comprises soaking Eurasian koi fish having a fish age of 1 to 3 dph in a range of 142 to 200 ppm. The concentration of ethinyl estradiol lasts from 1 to 3 minutes.

The above and other objects, features and advantages of the present invention will become apparent from

Detailed description of the invention

For the purposes of this specification, it will be clearly understood that the words "comprising" means "including but not limited to" and the words "comprises" have a corresponding meaning.

It is to be understood that if any of the previous publications is quoted here, the prior publication does not constitute an acknowledgement that in Taiwan or any other country, the former publication forms a common general in the art. Part of the knowledge.

All technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the invention pertains, unless otherwise defined. A person skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which can be used to practice the invention. Of course, the invention is in no way limited by the methods and materials described.

In order to develop a soaking process that is convenient and fast to operate and has a stable female rate, Applicants have found that the use of 142 to 200 ppm of ethinyl estradiol to soak the Eurasian squid fry can effectively induce females.

Accordingly, the present invention provides a method for inducing femaleization of Eurasian mullet, which comprises soaking Eurasian koi fish of 1 to 3 dph in a range of 142 to 200 ppm. The concentration of ethinyl estradiol lasts from 1 to 3 minutes.

Preferably, the Oulu kooka fish has a fish age of 3 dph.

Preferably, the concentration of ethinyl estradiol is 200 ppm.

According to the invention, the soaking can be carried out at 25 to 30 °C. In a preferred embodiment of the invention, the soaking is carried out at 25 °C.

According to the present invention, the femaleized Eurasian koi fish (ZZ ♀ ♀) (i.e., F1 progeny) obtained according to the method of the present invention can be taken with a wild-type male European Leah Wu Guoyu (ZZ♂) mated to obtain the male-red Eurasian Wu Guoyu (ZZ♂) (also known as the F2 progeny). The all-male Eurasian koi fish (ZZ♂) can be directly used as food, or used to produce all-male tilapia hybrids (XZ♂), which The operation procedure is as follows: firstly, an masculinized Nile koi fish (XX ♂ ♂) obtained by a conventional technique of the technical field to which the present invention pertains is mated with a wild-type female Nile koi fish (XX ♀). In order to obtain the whole female Nile Wu Guoyu (XX♀). Next, the whole female Nile Wu Guoyu (XX♀) is mated with the whole male Eurasian Wu Guoyu (ZZ♂) (that is, the F2 progeny), and the whole male Wu Guoyu can be obtained. Hybrid (XZ♂) (also known as F3 progeny).

Detailed description of the preferred embodiment

The invention is further described in the following examples, but it should be understood that these examples are for illustrative purposes only and are not to be construed as limiting. Odia tilapia A, 3 dph prepared: Example Example 1 using ethinyl estradiol (ethinyl estradiol) of the soaking treatment to induce Odia Tilapia (Tilapia aurea) feminization (feminization) Experimental Materials embodiment Fish fry:

In the present example, the Olympus squid male and female fish (fish age 12 months or older) used as a parent fish are the Department of Aquatic Biosciences at National. Provided by Professor Guo Jianxian of Chiayi University. The male fish and the female fish were respectively housed in a cement pool maintained at a water temperature of 25 ° C and a dissolved oxygen (DO) maintained at 6 mg/L or more under natural light, and the feed was sufficiently supplied. At the time of mating, the male and female fish are carried out at a ratio of 2:5. The fertilized egg is collected by rinsing the mouth of the female fish, and then cultured at 25 ° C for 7 to 14 days. In order to get 3 dph fry. Experimental methods and results:

An appropriate amount of ethinyl estradiol was added to 10 mL of ethanol, which was then added to a water tank containing 5 L of water and mixed so that the tank contained the desired concentration of ethinyl estradiol. Next, 3 dph of Olia squid fry was placed in the water tank at 25 ° C for 3 minutes. Thereafter, the induced fry are placed in a water tank provided with oxygen-enriched equipment for cultivation. After 7 months, the sex of all surviving fish was observed and the percentage of female was calculated. The test was carried out a total of 5 times, and the ethinyl estradiol concentration and the number of fry used in each test and the resulting female rate are shown in Table 1 below. Table 1. Concentration of ethinyl estradiol used in the test and the number of fry and the resulting female rate  <TABLE border="1" borderColor="#000000" width="85%"><TBODY><tr><td> Test</td><td> ethinyl estradiol (ppm) </td><td > Number of fry</td><td> Female rate (%) </td></tr><tr><td> 1 </td><td> 200 </td><td> 506 </td> <td> 100 </td></tr><tr><td> 2 </td><td> 300 </td><td> 100 </td></tr><tr><td> 3 </td><td> 421 </td><td> 100 </td></tr><tr><td> 4 </td><td> 367 </td><td> 100 </td ></tr><tr><td> 5 </td><td> 142.85 </td><td> 300 </td><td> 87 </td></tr></TBODY></ TABLE>

As can be seen from Table 1, the female rate of each test was about 87% or more, and in particular, when the concentration of ethinyl estradiol was 200 ppm, a female rate of up to 100% was obtained. In contrast, in the general case of untreated estrogen, the females of males mated with females should have a female rate of 50%. The results of this experiment showed that the use of 142.85 to 200 ppm of ethinyl estradiol to soak the Eurasian squid fry can effectively induce females.

In addition, the applicant further takes the female Eurasian koi fish obtained by immersing in 200 ppm of ethinyl estradiol with the male Eurasian koi fish in a ratio of 5:2. Mating, and the male rate of the obtained offspring is as high as 95% to 100%. The results of this experiment show that the female Eurasian koi fish obtained according to the method of the present invention can be used for the production of the whole male Eurasian koi fish.

All of the patents and documents cited in this specification are hereby incorporated by reference in their entirety. In the event of a conflict, the detailed description of the case (including definitions) will prevail.

While the invention has been described with respect to the specific embodiments of the invention, it will be understood that many modifications and changes can be made without departing from the scope and spirit of the invention. It is therefore intended that the invention be limited only by the scope of the appended claims.

Claims (3)

A method for inducing femaleization of Eurasian mullet, comprising: soaking Eurasian koi fish of 3dph in a lysine having a concentration ranging from 142 to 200 ppm for a duration of time 3 minutes. The method of claim 1, wherein the concentration of ethinyl estradiol is 200 ppm. The method of claim 1, wherein the soaking is performed at 25 °C.