CN116472953A - Potato crossbreeding method and device - Google Patents
Potato crossbreeding method and device Download PDFInfo
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- CN116472953A CN116472953A CN202310483834.1A CN202310483834A CN116472953A CN 116472953 A CN116472953 A CN 116472953A CN 202310483834 A CN202310483834 A CN 202310483834A CN 116472953 A CN116472953 A CN 116472953A
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- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 39
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 30
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- 238000002347 injection Methods 0.000 claims abstract description 19
- 239000007924 injection Substances 0.000 claims abstract description 19
- 230000007246 mechanism Effects 0.000 claims abstract description 19
- 239000002689 soil Substances 0.000 claims abstract description 17
- 238000005286 illumination Methods 0.000 claims abstract description 12
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 10
- 239000004576 sand Substances 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims description 17
- 230000010152 pollination Effects 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 229930191978 Gibberellin Natural products 0.000 claims description 6
- 235000021028 berry Nutrition 0.000 claims description 6
- 230000018109 developmental process Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 230000035784 germination Effects 0.000 claims description 6
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 6
- 239000003448 gibberellin Substances 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 3
- 239000004166 Lanolin Substances 0.000 claims description 3
- 206010040047 Sepsis Diseases 0.000 claims description 3
- 230000002939 deleterious effect Effects 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 229940039717 lanolin Drugs 0.000 claims description 3
- 235000019388 lanolin Nutrition 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 210000003097 mucus Anatomy 0.000 claims description 3
- 230000035772 mutation Effects 0.000 claims description 3
- 239000002674 ointment Substances 0.000 claims description 3
- 230000035699 permeability Effects 0.000 claims description 3
- 230000029553 photosynthesis Effects 0.000 claims description 3
- 238000010672 photosynthesis Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 208000013223 septicemia Diseases 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000009396 hybridization Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
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- 244000017020 Ipomoea batatas Species 0.000 description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 101100298225 Caenorhabditis elegans pot-2 gene Proteins 0.000 description 1
- 240000008892 Helianthus tuberosus Species 0.000 description 1
- 235000003230 Helianthus tuberosus Nutrition 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
- A01G27/003—Controls for self-acting watering devices
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0295—Units comprising two or more connected receptacles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/28—Raised beds; Planting beds; Edging elements for beds, lawn or the like, e.g. tiles
Abstract
The utility model discloses a potato crossbreeding device, which comprises a moisturizing mechanism and an illumination mechanism, wherein the moisturizing mechanism comprises a water pipe, a soil holding unit and an air holding unit, the soil holding unit comprises a seedling raising basin and a plurality of water injection pipes, part of the water pipe is buried underground, the water injection pipes are fixedly connected to the water pipe, a humidity sensor is fixedly connected to the periphery of the water injection pipes, sand loam is filled in the seedling raising basin after the water injection pipes extend into the seedling raising basin, the air holding unit comprises a plurality of atomizing nozzles, part of the water pipe is arranged above the seedling raising basin, the water pipe positioned above is 2m-2.5m away from the seedling raising basin, and the atomizing nozzles are fixedly connected to the bottom of the water pipe above; the utility model ensures the humidity of sand soil, the humidity and the illuminance of the whole environment in the hybridization process through the moisturizing mechanism and the illumination mechanism in the breeding device, thereby ensuring the applicability of the whole environment in the hybridization process and improving the seedling quality.
Description
Technical Field
The utility model relates to the technical field of potato planting, in particular to a potato cross breeding method and device.
Background
Potatoes belong to annual herbs of the Solanaceae family, tubers are edible, are the fourth most important food crops worldwide, and are next to wheat, rice and corn. Potato is also known as yam egg, potato, sweet potato, jerusalem artichoke, sweet potato, sun potato, egg, potato, etc. With wheat, rice, corn, sorghum and known as five crops worldwide. Potato tubers contain a large amount of starch, can provide abundant calories for humans, and are rich in proteins, amino acids, and various vitamins, minerals, especially their vitamin content is the most abundant in all food crops.
The potato needs to maintain warmer environment when growing seedlings, still needs to keep moist simultaneously, and current device of growing seedlings can accomplish this very well, but in current breeding mode, for example: the utility model discloses Chinese utility model (application number: CN 201920709741.5) high-efficiency intelligent potato seedling raising equipment, which comprises a box body, wherein a cavity I is fixedly connected to the center of an inner cavity of the box body, a top cover is fixedly connected to the left side of the top of the cavity I, a vent hole is fixedly connected to the left side of the top cover, a nutrient solution buffer box is fixedly connected to the bottom of the top cover, a nutrient solution conveying pipe is fixedly connected to the center of one side of the nutrient solution buffer box, and a water storage tank is fixedly connected to the bottom of the nutrient solution buffer box; the outside air can enter the seedling raising box through the arranged vent holes, so that the internal air condition is improved; the water storage tank and the water delivery pipe are arranged, so that nutrients and moisture required by seedlings can be effectively stored, and when the water storage tank and the water delivery pipe are used, although the water and the fertilizer can be finely managed, a large amount of water and fertilizer are wasted in soil with a large distance from plants, the plants cannot be well absorbed, and the passageways are muddy, so that a large amount of water and fertilizer resources are wasted, the environment of the breeding bottom surface of the greenhouse is bad, and scientific research work is inconvenient to develop.
Disclosure of Invention
The utility model aims to provide a potato crossbreeding method and device.
In order to achieve the above object, the technical scheme of the present utility model is as follows:
a potato crossbreeding method comprising the steps of:
step S1: parent selection, the parent comprising a male parent and a female parent, the male parent and the female parent being selected from a parent sample by the proportion of the same deleterious mutation between the male parent and the female parent;
step S2: selecting soil, namely selecting loose sandy loam with good storage property and permeability to form a nursery, wherein the nursery is positioned in a greenhouse;
step S3: the male parent and the female parent are respectively planted in different nursery garden, and the plant spacing is between 0.5m and 1 m;
step S4: when the potato plant grows to 15cm-20cm, removing stolon on the potato plant, and removing tubers expanding at the bottom of the plant from the bud period of the potato, so that nutrients generated by photosynthesis and assimilation of the plant are concentrated to the overground part of the plant, and the water content of soil is kept at 60% -67%;
step S5: when the potato plant enters into the flowering period, removing the flowers with the septicemia and the hypoplasia in the inflorescence, and removing stamens in the flower buds to be opened;
step S6: collecting pollen of the male parent, placing the pollen in a centrifuge tube of 1.5L at-20C for preservation, and marking the serial number and the freezing date of the male parent on the centrifuge tube;
step S7: selecting inflorescences with good development on female parent plants, leaving 3-6 flowers with moderate development on each inflorescences, emasculating anthers by forceps, and reserving stigmas;
step S7: removing pollen of male parent, smearing on castration stigma, pollinating, smearing 0.25mg/L naphthalene acetic acid lanolin ointment on the outside of flower stalk, and isolating on stigma with isolating tube;
step S8: after pollination, a label marked with a hybrid combination name is hung on the inflorescence, and the pollination time is noted; bagging after pollination for 2 weeks, and harvesting after berries are ripe;
step S9: collecting seeds, putting the softened berries in a beaker filled with distilled water, extruding the seeds, removing pulp and peel and mucus on the seeds, then putting the seeds on filter paper for airing, bagging the fully dried seeds after 2d to indicate the combination name and time, and storing at the temperature of minus 20 ℃;
step S10: placing hybrid seeds on water-absorbing paper in a glass culture dish according to a combination, soaking the seeds for 24 hours by using 1000mg/L gibberellin, pouring gibberellin solution, repeatedly flushing for 3 times by using distilled water, and then adding a proper amount of water into the culture dish to keep the seeds moist; and accelerating germination under the dark condition at room temperature until the germination is white.
Further, the PH of the sandy loam in the step S2 is between 6 and 8, the sand content of the sandy loam is between 30 and 35 percent, and the grain size of the sand is between 1 and 0.05 millimeter.
Further, the water content of the sandy loam in the step S2 is 55% -65%.
Further, in the steps S5-S7, the ambient temperature is 20-25 ℃, the humidity is 70% -75%, and the illumination time is not less than 16h/d.
Further, in step S3, the sowing ratio of the male parent to the female parent is 1:4.
The utility model provides a potato crossbreeding device, includes moisturizing mechanism, illumination mechanism, moisturizing mechanism includes raceway, soil keeps unit, air and keeps the unit, soil keeps the unit including educating seedling basin, a plurality of water injection pipe, and partial raceway is buried and underground, water injection pipe fixed connection is on the raceway, fixedly connected with humidity transducer on the periphery of water injection pipe, the water injection pipe stretches into educate seedling basin intussuseption after educating seedling basin and fills sandy loam, air keeps the unit to include a plurality of atomizer, and partial raceway is arranged in educating seedling basin's top, and is located the raceway of top and keep seedling basin 2m-2.5m apart from educating the raceway of seedling basin, atomizer fixed connection is the bottom of the raceway of top.
Further, the seedling raising basin comprises a bottom plate and a basin body, wherein the basin body is fixedly connected above the bottom plate, and the bottom plate is made of hard waterproof materials.
Further, the bottom of the seedling raising basin is provided with a perforation, the water injection pipe penetrates through the perforation and then stretches into the seedling raising basin, and the bottom of the seedling raising basin is provided with a water leakage hole.
Further, the illumination mechanism comprises a supporting rod and a plurality of fluorescent lamps, wherein the fluorescent lamps are fixedly connected to the supporting rod.
Compared with the prior art, the utility model has the advantages and positive effects that:
the utility model ensures the humidity of sand soil, the humidity and the illuminance of the whole environment in the hybridization process through the moisturizing mechanism and the illumination mechanism in the breeding device, thereby ensuring the applicability of the whole environment in the hybridization process and improving the seedling quality.
According to the utility model, the seedling raising range can be controlled as much as possible by planting parents in the seedling raising basin, so that the consumption of water resources and fertilizer resources is reduced, the smoothness of the passageway in the greenhouse is ensured, the road muddy caused by flood irrigation is avoided, and the comfort in seedling raising of scientific researchers is improved.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the utility model, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a front view of the present utility model;
fig. 2 is an enlarged view of fig. 1 at a in accordance with the present utility model.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in figures 1-2 of the drawings,
a potato crossbreeding method comprising the steps of:
step S1: parent selection, the parent comprising a male parent and a female parent, the male parent and the female parent being selected from a parent sample by the proportion of the same deleterious mutation between the male parent and the female parent;
step S2: selecting soil, namely selecting loose sandy loam with good storage property and permeability to form a nursery, wherein the nursery is positioned in a greenhouse;
step S3: the male parent and the female parent are respectively planted in different nursery garden, and the plant spacing is between 0.5m and 1 m;
step S4: when the potato plant grows to 15cm-20cm, removing stolon on the potato plant, and removing tubers expanding at the bottom of the plant from the bud period of the potato, so that nutrients generated by photosynthesis and assimilation of the plant are concentrated to the overground part of the plant, and the water content of soil is kept at 60% -67%;
step S5: when the potato plant enters into the flowering period, removing the flowers with the septicemia and the hypoplasia in the inflorescence, and removing stamens in the flower buds to be opened;
step S6: collecting pollen of the male parent, placing the pollen in a centrifuge tube of 1.5L at-20C for preservation, and marking the serial number and the freezing date of the male parent on the centrifuge tube;
step S7: selecting inflorescences with good development on female parent plants, leaving 3-6 flowers with moderate development on each inflorescences, emasculating anthers by forceps, and reserving stigmas;
step S7: removing pollen of male parent, smearing on castration stigma, pollinating, smearing 0.25mg/L naphthalene acetic acid lanolin ointment on the outside of flower stalk, and isolating on stigma with isolating tube;
step S8: after pollination, a label marked with a hybrid combination name is hung on the inflorescence, and the pollination time is noted; bagging after pollination for 2 weeks, and harvesting after berries are ripe;
step S9: collecting seeds, putting the softened berries in a beaker filled with distilled water, extruding the seeds, removing pulp and peel and mucus on the seeds, then putting the seeds on filter paper for airing, bagging the fully dried seeds after 2d to indicate the combination name and time, and storing at the temperature of minus 20 ℃;
step S10: placing hybrid seeds on water-absorbing paper in a glass culture dish according to a combination, soaking the seeds for 24 hours by using 1000mg/L gibberellin, pouring gibberellin solution, repeatedly flushing for 3 times by using distilled water, and then adding a proper amount of water into the culture dish to keep the seeds moist; and accelerating germination under the dark condition at room temperature until the germination is white.
In this example, the step S2 sandy loam has a pH of between 6 and 8 and a sand content of between 30% and 35% sand, wherein the sand has a particle size of between 1 and 0.05 mm.
In this embodiment, the moisture content of the sandy loam in step S2 is 55% -65%.
In the embodiment, the ambient temperature is 20-25 ℃ and the humidity is 70-75% in the steps S5-S7, and the illumination time is not less than 16h/d.
In this embodiment, in step S3, the sowing ratio of the male parent to the female parent is 1:4.
The utility model provides a potato crossbreeding device, includes moisturizing mechanism, illumination mechanism, moisturizing mechanism includes raceway 1, soil keeps unit, air and keeps the unit, soil keeps the unit and includes nursery basin 2, a plurality of water injection pipe 3, and part raceway 1 is buried and underground, water injection pipe 3 fixed connection is on raceway 1, fixedly connected with humidity transducer 31 on the periphery of water injection pipe 3, nursery basin 2 intussuseption is filled with sandy loam 9 after water injection pipe 3 stretches into nursery basin 2, air keeps the unit and includes a plurality of atomizer 4, and part raceway 1 is arranged in the top of nursery basin 2, and is located the raceway 1 distance nursery basin 22m-2.5m of top, atomizer 4 fixed connection the bottom of raceway 1 of top.
In this embodiment, the seedling raising basin 2 includes a bottom plate and a basin body, the basin body is fixedly connected above the bottom plate, and the bottom plate is made of a hard waterproof material.
In this embodiment, a perforation 21 is provided at the bottom of the seedling raising basin 2, the water injection pipe 3 passes through the perforation 21 and then extends into the seedling raising basin 2, and a water leakage hole 22 is provided at the bottom of the seedling raising basin 2.
In this embodiment, the illumination mechanism includes a support rod 5 and a plurality of fluorescent lamps 6, and the fluorescent lamps 6 are fixedly connected to the support rod 4.
In this embodiment, the temperature in the environment may be detected using a thermometer suspended within the greenhouse.
In this embodiment, the lower part of the seedling raising pot 2 is placed in the land 8.
All other embodiments, modifications, equivalents, improvements, etc., which are apparent to those skilled in the art without the benefit of this disclosure, are intended to be included within the scope of this utility model.
Claims (9)
1. A potato crossbreeding method, comprising the steps of:
step S1: parent selection, the parent comprising a male parent and a female parent, the male parent and the female parent being selected from a parent sample by the proportion of the same deleterious mutation between the male parent and the female parent;
step S2: selecting soil, namely selecting loose sandy loam with good storage property and permeability to form a nursery, wherein the nursery is positioned in a greenhouse;
step S3: the male parent and the female parent are respectively planted in different nursery garden, and the plant spacing is between 0.5m and 1 m;
step S4: when the potato plant grows to 15cm-20cm, removing stolon on the potato plant, and removing tubers expanding at the bottom of the plant from the bud period of the potato, so that nutrients generated by photosynthesis and assimilation of the plant are concentrated to the overground part of the plant, and the water content of soil is kept at 60% -67%;
step S5: when the potato plant enters into the flowering period, removing the flowers with the septicemia and the hypoplasia in the inflorescence, and removing stamens in the flower buds to be opened;
step S6: collecting pollen of the male parent, placing the pollen in a centrifuge tube of 1.5L at-20C for preservation, and marking the serial number and the freezing date of the male parent on the centrifuge tube;
step S7: selecting inflorescences with good development on female parent plants, leaving 3-6 flowers with moderate development on each inflorescences, emasculating anthers by forceps, and reserving stigmas;
step S7: removing pollen of male parent, smearing on castration stigma, pollinating, smearing 0.25mg/L naphthalene acetic acid lanolin ointment on the outside of flower stalk, and isolating on stigma with isolating tube;
step S8: after pollination, a label marked with a hybrid combination name is hung on the inflorescence, and the pollination time is noted; bagging after pollination for 2 weeks, and harvesting after berries are ripe;
step S9: collecting seeds, putting the softened berries in a beaker filled with distilled water, extruding the seeds, removing pulp and peel and mucus on the seeds, then putting the seeds on filter paper for airing, bagging the fully dried seeds after 2d to indicate the combination name and time, and storing at the temperature of minus 20 ℃;
step S10: placing hybrid seeds on water-absorbing paper in a glass culture dish according to a combination, soaking the seeds for 24 hours by using 1000mg/L gibberellin, pouring gibberellin solution, repeatedly flushing for 3 times by using distilled water, and then adding a proper amount of water into the culture dish to keep the seeds moist; and accelerating germination under the dark condition at room temperature until the germination is white.
2. The potato crossbreeding method of claim 1, wherein: the PH of the sandy loam in the step S2 is between 6 and 8, the sand content of the sandy loam is between 30 and 35 percent, and the grain size of the sand is between 1 and 0.05 millimeter.
3. The potato crossbreeding method of claim 1, wherein: the water content of the sandy loam in the step S2 is 55-65%.
4. The potato crossbreeding method of claim 1, wherein: and step S5-step S7, wherein the ambient temperature is 20-25 ℃, the humidity is 70-75%, and the illumination time is not less than 16h/d.
5. The potato crossbreeding method of claim 1, wherein: in the step S3, the sowing ratio of the male parent to the female parent is 1:4.
6. A potato crossbreeding apparatus according to any one of claims 1-5, characterized in that: including moisturizing mechanism, illumination mechanism, moisturizing mechanism includes raceway, soil keeps unit, air and keeps the unit, soil keeps the unit including educating seedling tray, a plurality of water injection pipe, and part raceway is buried and underground, water injection pipe fixed connection is on the raceway, fixedly connected with humidity transducer on the periphery of water injection pipe, the water injection pipe stretches into educates seedling tray intussuseption after educating seedling tray and fills sandy loam, air keeps the unit including a plurality of atomizer, and part raceway is arranged in educating seedling tray's top, and is located the raceway distance of top educates seedling tray 2m-2.5m, atomizer fixed connection's bottom of the raceway of top.
7. The potato crossbreeding apparatus of claim 6, wherein: the seedling raising basin comprises a bottom plate and a basin body, wherein the basin body is fixedly connected above the bottom plate, and the bottom plate is made of hard waterproof materials.
8. The potato crossbreeding apparatus of claim 6, wherein: the bottom of the seedling raising basin is provided with a perforation, the water injection pipe penetrates through the perforation and then stretches into the seedling raising basin, and the bottom of the seedling raising basin is provided with a water leakage hole.
9. The potato crossbreeding apparatus of claim 6, wherein: the illumination mechanism comprises a supporting rod and a plurality of fluorescent lamps, and the fluorescent lamps are fixedly connected to the supporting rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310483834.1A CN116472953A (en) | 2023-04-28 | 2023-04-28 | Potato crossbreeding method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310483834.1A CN116472953A (en) | 2023-04-28 | 2023-04-28 | Potato crossbreeding method and device |
Publications (1)
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