CN116439165A - Breeding method for improving survival rate of coral larvae - Google Patents
Breeding method for improving survival rate of coral larvae Download PDFInfo
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- CN116439165A CN116439165A CN202310488217.0A CN202310488217A CN116439165A CN 116439165 A CN116439165 A CN 116439165A CN 202310488217 A CN202310488217 A CN 202310488217A CN 116439165 A CN116439165 A CN 116439165A
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- 235000014653 Carica parviflora Nutrition 0.000 title claims abstract description 53
- 230000004083 survival effect Effects 0.000 title claims abstract description 24
- 238000009395 breeding Methods 0.000 title claims description 4
- 241000243321 Cnidaria Species 0.000 title description 33
- 239000002131 composite material Substances 0.000 claims abstract description 25
- 235000015097 nutrients Nutrition 0.000 claims abstract description 21
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 20
- 238000012364 cultivation method Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 8
- 241000588770 Proteus mirabilis Species 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 230000003203 everyday effect Effects 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 235000016709 nutrition Nutrition 0.000 claims abstract description 3
- 230000001105 regulatory effect Effects 0.000 claims abstract description 3
- 244000132059 Carica parviflora Species 0.000 claims abstract 18
- -1 compound salt Chemical class 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 235000013343 vitamin Nutrition 0.000 claims description 9
- 239000011782 vitamin Substances 0.000 claims description 9
- 229940088594 vitamin Drugs 0.000 claims description 9
- 229930003231 vitamin Natural products 0.000 claims description 9
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 7
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 7
- 241000238557 Decapoda Species 0.000 claims description 7
- 241000257465 Echinoidea Species 0.000 claims description 7
- 241000237536 Mytilus edulis Species 0.000 claims description 7
- 241000196252 Ulva Species 0.000 claims description 7
- 235000021323 fish oil Nutrition 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 7
- 235000020638 mussel Nutrition 0.000 claims description 7
- 239000013535 sea water Substances 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 150000004820 halides Chemical class 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 230000002354 daily effect Effects 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 230000001418 larval effect Effects 0.000 claims 1
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- 241000607598 Vibrio Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 244000052769 pathogen Species 0.000 abstract description 2
- 230000001717 pathogenic effect Effects 0.000 abstract description 2
- 150000003839 salts Chemical class 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 241000242757 Anthozoa Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
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- Animal Husbandry (AREA)
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Abstract
The invention provides a cultivation method for improving survival rate of coral larvae, which comprises the following steps: placing coral in a cultivation pool containing reefs, regulating the water temperature, pH value and salinity of the cultivation pool, giving light every day, and adding a microbial inoculum and a composite nutrient material for cultivation; the microbial inoculum consists of bacillus subtilis, rujieshi mobilis and proteus mirabilis and is cultured for 30-45 days. The microbial inoculum and the compound nutritional agent with specific formulas are applied to the cultivation pool containing the reefs, so that the vibrio activity is effectively inhibited, the coral larvae are protected from pathogen infection, the immunity, the heat resistance and the acid-base tolerance of the coral larvae are improved, the disease resistance of the coral larvae is improved, the growth of the coral larvae is promoted, and the survival rate of the coral larvae is improved.
Description
Technical Field
The invention relates to the field of coral culture, in particular to a culture method for improving survival rate of coral larvae.
Background
Coral grows in tropical or subtropical areas with low latitude, the total area accounts for 0.1% -0.5% of the area of the whole sea area, is a habitat of small marine organisms, has rich biological diversity, and is known as a tropical rain forest in the ocean. The coral worm belongs to the class of coral worm, has small volume and large quantity, and a plurality of coral worm are gathered together to form coral. However, coral has strict requirements on the growth environment, coral larvae are easily affected by temperature, water quality, illumination and bacteria in the initial period of cultivation, and the death rate is high in the first few weeks, so that the breeding expansion of the coral population is not facilitated, and the artificial repair and popularization of the coral are not facilitated.
Disclosure of Invention
Therefore, the invention provides a cultivation method for improving the survival rate of coral larvae.
The technical scheme of the invention is realized as follows:
a cultivation method for improving survival rate of coral larvae comprises the following steps: placing coral in a cultivation pool containing reefs, regulating the water temperature, pH value and salinity of the cultivation pool, giving light every day, and adding a microbial inoculum and a composite nutrient material for cultivation; the microbial inoculum consists of bacillus subtilis, rujieshi mobilis and proteus mirabilis and is cultured for 30-45 days.
Further, the microbial inoculum comprises the following components in percentage by mass of 1-2: 0.5 to 0.8:1.2 to 1.5 of bacillus subtilis, rujieshi mobilis and proteus mirabilis.
Further, the composite nutrient comprises the following raw materials in parts by weight: 200-300 parts of shrimp meal, 200-300 parts of mussels, 100-200 parts of sea urchin meat, 30-80 parts of ulva, 40-50 parts of kelp, 30-60 parts of chlorella, 1-2 parts of fish oil, 0.1-0.5 part of compound salt, 0.5-2 parts of compound amino acid, 1-3 parts of compound vitamin and 900-1500 parts of seawater.
Further, the processing method of the composite nutrient comprises the following steps: grinding shrimp powder, mussel, sea urchin meat, ulva, kelp, chlorella, compound salt, compound amino acid, compound vitamin and seawater in a grinder, squeezing with gauze, and filtering to obtain mixed solution; adding fish oil into the mixed solution to obtain the composite nutrient.
Further, the composite salt comprises the following components in percentage by mass of 0.1-0.5: 0.01 to 0.05 of calcium carbonate and magnesium sulfate.
Further, the compound amino acid and compound vitamin manufacturer is ruifan.
Further, the application frequency of the microbial inoculum is 10-15 days, and the application amount of the microbial inoculum is 2-5g/m each time 2 。
Further, the application frequency of the composite nutrient is 1-2 days, and the application amount of the composite nutrient is 100-300g/m each time 2 。
Further, the water temperature of the cultivation pool is 25-28 ℃; the pH value is 8.0-8.2; the salinity is 30-35 per mill.
Further, light is given every day, and the specific conditions of the light are as follows: the power of 160-240W and color temperature of 10000-15000K are used as the halide lamp, and the illumination time per day is 7-12h.
Compared with the prior art, the invention has the beneficial effects that: by adopting the method, the bacterial agent and the compound nutritional agent with specific formulas are applied in the cultivation pool containing reefs, so that the activity of vibrio is effectively inhibited, the coral larvae are protected from pathogen infection, the disease resistance, heat resistance and acid-base tolerance of the coral larvae are improved, the immunity of the coral larvae is improved, the growth of the coral larvae is promoted, and the survival rate of the coral larvae is improved.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
Experimental example 1-cultivation method for improving survival rate of coral larvae
Placing 80 strains of coral in culture ponds containing reefs, wherein the volume of each culture pond is 9m multiplied by 1.5m, adjusting the water temperature of the culture pond to 26 ℃, the pH value to 8.0 and the salinity to 32 per mill, using a halide lamp with the power of 200W and the color temperature of 12000K, and giving illumination for 8h each day; the microbial inoculum is applied once every 10 days, and the application amount of the microbial inoculum is 2g/m 2 The method comprises the steps of carrying out a first treatment on the surface of the The composite nutrient is applied once daily, and the application amount of the composite nutrient is 150g/m each time 2 Culturing for 40 days; the microbial inoculum consists of the following components in percentage by mass: 0.5:1.2, bacillus subtilis, rujieshi mobilis and proteus mirabilis; the composite nutrient material is prepared from the following raw materials in parts by weight: 200 parts of shrimp meal, 240 parts of mussel, 100 parts of sea urchin meat, 30 parts of ulva, 45 parts of kelp, 50 parts of chlorella, 1 part of fish oil, 0.1 part of compound salt, 0.2 part of compound amino acid, 1 part of compound vitamin and 1000 parts of seawater; the mass ratio of the composite salt is 0.2:0.04 of calcium carbonate and magnesium sulfate.
Experimental example 2-cultivation method for improving survival rate of coral larvae
Placing 100 corals in culture ponds containing reefs, wherein the volume of each culture pond is 10m×10m×1.5m, adjusting the water temperature of the culture pond to 26 ℃, the pH value to 8.0 and the salinity to 32%o, using a halide lamp with the power of 200W and the color temperature of 12000K, and giving illumination for 10h each day; the microbial inoculum is applied once every 12 days, and the application amount of the microbial inoculum is 3g/m 2 The method comprises the steps of carrying out a first treatment on the surface of the The composite nutrient is applied once daily, and the application amount of the composite nutrient is 200g/m each time 2 Culturing for 42 days; the microbial inoculum consists of the following components in percentage by mass of 1.5:0.5:1.3, bacillus subtilis, rujieshi mobilis and proteus mirabilis; the composite nutrient material is prepared from the following raw materials in parts by weight: 260 parts of shrimp powder, 270 parts of mussel, 150 parts of sea urchin meat, 50 parts of ulva, 45 parts of kelp, 50 parts of chlorella, 1 part of fish oil, 0.2 part of compound salt, 1.2 parts of compound amino acid, 2 parts of compound vitamin and 1000 parts of seawater; the mass ratio of the composite salt is 0.2:0.03 of calcium carbonate and magnesium sulfate.
Experimental example 3-cultivation method for improving survival rate of coral larvae
Placing 100 corals in culture ponds containing reefs, wherein the volume of each culture pond is 10m×10m×1.5m, adjusting the water temperature of the culture pond to 26 ℃, the pH value to 8.0 and the salinity to 32%o, using a halide lamp with the power of 240W and the color temperature of 15000K, and giving illumination for 10h each day; the microbial inoculum is applied once every 12 days, and the application amount of the microbial inoculum is 5g/m 2 The method comprises the steps of carrying out a first treatment on the surface of the The composite nutrient is applied once daily, and the application amount of the composite nutrient is 300g/m each time 2 Culturing for 45 days; the microbial inoculum consists of the following components in percentage by mass: 0.7:1.5, bacillus subtilis, brevibacterium mobilis and Proteus mirabilis; the composite nutrient material is prepared from the following raw materials in parts by weight: 300 parts of shrimp meal, 300 parts of mussels, 200 parts of sea urchin meat, 70 parts of ulva, 50 parts of kelp, 50 parts of chlorella, 1 part of fish oil, 0.5 part of compound salt, 2 parts of compound amino acid, 3 parts of compound vitamin and 1500 parts of seawater; the mass ratio of the composite salt is 0.3:0.05 of calcium carbonate and magnesium sulfate.
Coral was cultivated by the method of experimental examples 1 to 3, and the coral was observed on day 0, day 7, day 20 and day 40, the amount of coral whitening was calculated, and the survival rate of coral larvae was measured, and the experimental results were as follows:
TABLE 1 coral larva survival rate (%)
| Day 0 | Day 7 | Day 20 | Day 40 | |
| Experimental example 1 | 99.8 | 98.3 | 96.5 | 95.2 |
| Experimental example 2 | 99.9 | 99.1 | 97.9 | 95.6 |
| Experimental example 3 | 99.7 | 98.0 | 96.2 | 95.1 |
Experimental results show that the survival rate of coral larvae is over 95% when the coral larvae are cultivated on the 40 th day by adopting the coral larva cultivation method.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (10)
1. A breeding method for improving survival rate of coral larvae is characterized by comprising the following steps: placing coral in a cultivation pool containing reefs, regulating the water temperature, pH value and salinity of the cultivation pool, giving light every day, and adding a microbial inoculum and a composite nutrient material for cultivation; the microbial inoculum consists of bacillus subtilis, rujieshi mobilis and proteus mirabilis and is cultured for 30-45 days.
2. The cultivation method for improving the survival rate of coral larvae according to claim 1, wherein the microbial inoculum comprises the following components in percentage by mass: 0.5 to 0.8:1.2 to 1.5 of bacillus subtilis, rujieshi mobilis and proteus mirabilis.
3. The cultivation method for improving the survival rate of coral larvae as claimed in claim 1, wherein the compound nutritional material is prepared from the following raw materials in parts by weight: 200-300 parts of shrimp meal, 200-300 parts of mussels, 100-200 parts of sea urchin meat, 30-80 parts of ulva, 40-50 parts of kelp, 30-60 parts of chlorella, 1-2 parts of fish oil, 0.1-0.5 part of compound salt, 0.5-2 parts of compound amino acid, 1-3 parts of compound vitamin and 900-1500 parts of seawater.
4. A method of raising coral larvae survival as claimed in claim 3, wherein the method of processing the composite nutrient comprises the steps of: grinding shrimp powder, mussel, sea urchin meat, ulva, kelp, chlorella, compound salt, compound amino acid, compound vitamin and seawater in a grinder, squeezing with gauze, and filtering to obtain mixed solution; adding fish oil into the mixed solution to obtain the composite nutrient.
5. A method of raising coral larva survival rate according to claim 3, wherein the compound salt comprises the following components in mass ratio of 0.1-0.5: 0.01 to 0.05 of calcium carbonate and magnesium sulfate.
6. A method of raising coral larvae survival as claimed in claim 3, wherein the compound amino acid and compound vitamin manufacturer is RUIFINE.
7. A method of raising coral larvae survival rate as claimed in claim 2, wherein the bacterial agent is applied at a frequency of from 10 to 15 days at an amount of from 2 to 5g/m per time 2 。
8. An improvement of coral larval survival rate as claimed in claim 3The cultivation method is characterized in that the application frequency of the composite nutrient is 1-2 days, and the application amount of the composite nutrient is 100-300g/m each time 2 。
9. A method of raising coral larvae survival rate as claimed in claim 1, wherein the temperature of water in the culture pond is 25-28 ℃; the pH value is 8.0-8.2; the salinity is 30-35 per mill.
10. A method of raising coral larvae survival as claimed in claim 1, wherein light is given daily under the following conditions: the power of 160-240W and color temperature of 10000-15000K are used as the halide lamp, and the illumination time per day is 7-12h.
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|---|---|---|---|---|
| JP2005323593A (en) * | 2004-04-14 | 2005-11-24 | National Institute Of Advanced Industrial & Technology | Method and apparatus for cultivating useful marine organism on land, and useful marine organism obtained by the apparatus and the method |
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