CN104498409A - Method for culturing rhodospirillum for degrading organic contaminations of aquiculture - Google Patents

Method for culturing rhodospirillum for degrading organic contaminations of aquiculture Download PDF

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Publication number
CN104498409A
CN104498409A CN201410848190.2A CN201410848190A CN104498409A CN 104498409 A CN104498409 A CN 104498409A CN 201410848190 A CN201410848190 A CN 201410848190A CN 104498409 A CN104498409 A CN 104498409A
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China
Prior art keywords
rhodospirillum
aquaculture
substratum
sodium
days
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CN201410848190.2A
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Chinese (zh)
Inventor
季延滨
李涛
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TIANJIN ZHENBANG AQUACULTURE Co Ltd
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TIANJIN ZHENBANG AQUACULTURE Co Ltd
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Priority to CN201410848190.2A priority Critical patent/CN104498409A/en
Publication of CN104498409A publication Critical patent/CN104498409A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The invention provides a method for culturing rhodospirillum for degrading organic contaminations of aquiculture. The method comprises the steps of breeding salt tolerance of rhodospirillum and sulfur-free rhodospirillum, obtaining salt-tolerant blended rhodospirillum strains according to a gradient cultivation method and culturing salt-tolerant blended rhodospirillum. The method for culturing rhodospirillum for degrading organic contaminations of aquiculture disclosed by the invention has the beneficial effects that the salt-tolerant blended rhodospirillum has no toxicity to aquatic animals and no side and toxicity to human bodies, is capable of effectively improving the conditions of water areas for aquiculture, is simple in use, and has good application prospect; and the salt-tolerant blended rhodospirillum obtained according to the method disclosed by the invention and added in a growth water area of aquatic animals can be used for effectively reducing the content of organic contaminations in the bottom mud of the water area, so that the environment of the culture water area is beneficial to the growth of aquatic animals such as fish, shrimps and crabs.

Description

For the rhodospirillum cultural method of aquaculture degradable organic pollutant
Technical field
The present invention relates to field of microbial culture technology, more specifically to a kind of rhodospirillum cultural method for aquaculture degradable organic pollutant.
Background technology
Aquaculture is the activity in production of breeding, cultivating and gather in the crops aquatic animals and plants under manual control.Generally comprise the whole process forming fishery products under artificial breeding management from seed.Broadly also Enhancement of fishery resources can be comprised.Aquaculture has the modes such as extensive cultivation, intensive culture and high density culture.Extensive cultivation throws in seed in medium and small natural water area, forms fishery products completely by natural bait, as lake and reservoir breed fish and shallow sea support shellfish etc.Intensive culture forms fishery products with bait throwing in, fertilizing method, as aqiuculrue, cage culture and enclosure culture, net enclosure culture etc. in comparatively small size pond.High density culture adopts flowing water, temperature control, oxygenation and the methods such as high-quality bait of throwing something and feeding, and carries out high-density breeding in small size pond, thus obtains high yield, as Running Water Intensive Fish Farming, shrimp etc.
Rhodospirillum is lived in the mud in lake, pond, is a kind of typical amphitrophy type bacterium.When rhodospirillum grows under difficult environmental conditions, its nutrient type can change.Do not having under organic condition, it can utilize luminous energy, and stabilizing carbon dioxide fixes organism; Having under organic condition, it can utilize again organism to grow.According to its feature, start at present to use rhodospirillum to purify the organic waste water of high density in efforts at environmental protection, to reach protection of the environment, the object of decontamination.
Summary of the invention
Instant invention overcomes deficiency of the prior art, provide a kind of rhodospirillum cultural method for aquaculture degradable organic pollutant.
Object of the present invention is achieved by following technical proposals.
For the rhodospirillum cultural method of aquaculture degradable organic pollutant, carry out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 0.1-1.5g, SODIUM PHOSPHATE, MONOBASIC 0.2-2.7g, yeast extract 2.5-9.6g, sodium-acetate 2.7-6.8g, ammonium sulfate 0.1-1.5g, Sodium orthomolybdate 0.05-0.15g and boric acid 0.05-0.15g, PH is 6.5-7.8, cultivates 3-10 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in described step 1 by gradient method of cultivation, and after continuous passage 3-6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in described step 2, at 25-35 DEG C, intensity of illumination is continue under the condition of 10000-70000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.1-1.5g, magnesium sulfate 1.5-3.5g, L-glutamic acid 0.15-0.56g, ferrous sulfate 0.25-0.75g and aquaculture seawater 0.5L, PH is 7.0-8.0, cultivates 9-15 days, obtains salt tolerant mixture of red spirillum.
In described step 1, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 1.2g, SODIUM PHOSPHATE, MONOBASIC 1.9g, yeast extract 4.8g, sodium-acetate 5.6g, ammonium sulfate 0.8g, Sodium orthomolybdate 0.09g and boric acid 0.09g, PH are 7.4, and described incubation time is 7 days.
In described step 2, described continuous passage was 5 generations.
In described step 3, described culture condition is: culture temperature is 30 DEG C, intensity of illumination is 30000LX, described substratum consists of: aquaculture waters bed mud 0.5L, ammonium chloride 1.2g, magnesium sulfate 2.6g, L-glutamic acid 0.36g, ferrous sulfate 0.56g and aquaculture seawater 0.5L, PH are 7.4, described incubation time 10 days.
Beneficial effect of the present invention is: this salt tolerant mixture of red spirillum, to hydrocoles nontoxicity, to human non-toxic's side effect, and effectively improves the water area condition in aquaculture waters, and application method is simple, has a good application prospect; The salt tolerant mixture of red spirillum that the inventive method obtains is added with in fishery products growth waters, effectively reduce the content of this Cultivated water Organic Pollutants in Sedimentary Mud, make the growth of the fish of environmental benefits in aquaculture, shrimp, crab etc. the fishery products of this Cultivated water.
Embodiment
Below by specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
For the rhodospirillum cultural method of aquaculture degradable organic pollutant, carry out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 0.1g, SODIUM PHOSPHATE, MONOBASIC 0.2g, yeast extract 2.5g, sodium-acetate 2.7g, ammonium sulfate 0.1g, Sodium orthomolybdate 0.05g and boric acid 0.05g, PH is 7.8, cultivates 10 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, and after continuous passage 6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in step 2, at 35 DEG C, intensity of illumination is continue under the condition of 70000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.1g, magnesium sulfate 1.5g, L-glutamic acid 0.15g, ferrous sulfate 0.25g and aquaculture seawater 0.5L, PH is 8.0, cultivates 15 days, obtains salt tolerant mixture of red spirillum.
Embodiment 2
For the rhodospirillum cultural method of aquaculture degradable organic pollutant, carry out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 1.5g, SODIUM PHOSPHATE, MONOBASIC 2.7g, yeast extract 9.6g, sodium-acetate 6.8g, ammonium sulfate 1.5g, Sodium orthomolybdate 0.15g and boric acid 0.15g, PH is 6.5, cultivates 3 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, and after continuous passage 3 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in step 2, at 25 DEG C, intensity of illumination is continue under the condition of 10000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 1.5g, magnesium sulfate 3.5g, L-glutamic acid 0.56g, ferrous sulfate 0.75g and aquaculture seawater 0.5L, PH is 7.0, cultivates 9 days, obtains salt tolerant mixture of red spirillum.
Embodiment 3
For the rhodospirillum cultural method of aquaculture degradable organic pollutant, carry out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 1.2g, SODIUM PHOSPHATE, MONOBASIC 1.9g, yeast extract 4.8g, sodium-acetate 5.6g, ammonium sulfate 0.8g, Sodium orthomolybdate 0.09g and boric acid 0.09g, PH is 7.4, cultivates 7 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, and after continuous passage 5 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in step 2, at 30 DEG C, intensity of illumination is continue under the condition of 30000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 1.2g, magnesium sulfate 2.6g, L-glutamic acid 0.36g, ferrous sulfate 0.56g and aquaculture seawater 0.5L, PH is 7.4, cultivates 10 days, obtains salt tolerant mixture of red spirillum.
This salt tolerant mixture of red spirillum, to hydrocoles nontoxicity, to human non-toxic's side effect, and effectively improves the water area condition in aquaculture waters, and application method is simple, has a good application prospect; The salt tolerant mixture of red spirillum that the inventive method obtains is added with in fishery products growth waters, effectively reduce the content of this Cultivated water Organic Pollutants in Sedimentary Mud, make the growth of the fish of environmental benefits in aquaculture, shrimp, crab etc. the fishery products of this Cultivated water.
Above to invention has been exemplary description; should be noted that; when not departing from core of the present invention, any simple distortion, amendment or other those skilled in the art can not spend the equivalent replacement of creative work all to fall into protection scope of the present invention.

Claims (4)

1. for the rhodospirillum cultural method of aquaculture degradable organic pollutant, it is characterized in that: carry out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 0.1-1.5g, SODIUM PHOSPHATE, MONOBASIC 0.2-2.7g, yeast extract 2.5-9.6g, sodium-acetate 2.7-6.8g, ammonium sulfate 0.1-1.5g, Sodium orthomolybdate 0.05-0.15g and boric acid 0.05-0.15g, PH is 6.5-7.8, cultivates 3-10 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in described step 1 by gradient method of cultivation, and after continuous passage 3-6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in described step 2, at 25-35 DEG C, intensity of illumination is continue under the condition of 10000-70000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.1-1.5g, magnesium sulfate 1.5-3.5g, L-glutamic acid 0.15-0.56g, ferrous sulfate 0.25-0.75g and aquaculture seawater 0.5L, PH is 7.0-8.0, cultivates 9-15 days, obtains salt tolerant mixture of red spirillum.
2. the rhodospirillum cultural method for aquaculture degradable organic pollutant according to claim 1, it is characterized in that: in described step 1, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 1.2g, SODIUM PHOSPHATE, MONOBASIC 1.9g, yeast extract 4.8g, sodium-acetate 5.6g, ammonium sulfate 0.8g, Sodium orthomolybdate 0.09g and boric acid 0.09g, PH is 7.4, and described incubation time is 7 days.
3. the rhodospirillum cultural method for aquaculture degradable organic pollutant according to claim 1, is characterized in that: in described step 2, and described continuous passage was 5 generations.
4. the rhodospirillum cultural method for aquaculture degradable organic pollutant according to claim 1, it is characterized in that: in described step 3, described culture condition is: culture temperature is 30 DEG C, and intensity of illumination is 30000LX, and described substratum consists of: aquaculture waters bed mud 0.5L, ammonium chloride 1.2g, magnesium sulfate 2.6g, L-glutamic acid 0.36g, ferrous sulfate 0.56g and aquaculture seawater 0.5L, PH is 7.4, described incubation time 10 days.
CN201410848190.2A 2014-12-29 2014-12-29 Method for culturing rhodospirillum for degrading organic contaminations of aquiculture Pending CN104498409A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105296397A (en) * 2015-11-12 2016-02-03 蚌埠市水森林生态农业科技园 Method for culturing aquaculture rhodospirillum
CN109479766A (en) * 2018-11-14 2019-03-19 浙江海洋大学 Bream artificial cultivating method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105296397A (en) * 2015-11-12 2016-02-03 蚌埠市水森林生态农业科技园 Method for culturing aquaculture rhodospirillum
CN109479766A (en) * 2018-11-14 2019-03-19 浙江海洋大学 Bream artificial cultivating method

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Application publication date: 20150408