CN116368218A - 分化诱导为多能干细胞的外胚层、中胚层和内胚层系细胞的方法 - Google Patents

分化诱导为多能干细胞的外胚层、中胚层和内胚层系细胞的方法 Download PDF

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CN116368218A
CN116368218A CN202180070010.0A CN202180070010A CN116368218A CN 116368218 A CN116368218 A CN 116368218A CN 202180070010 A CN202180070010 A CN 202180070010A CN 116368218 A CN116368218 A CN 116368218A
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门田纯平
今泉裕
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Tosoh Corp
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CN202180070010.0A 2020-08-18 2021-08-17 分化诱导为多能干细胞的外胚层、中胚层和内胚层系细胞的方法 Pending CN116368218A (zh)

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JP2020-138106 2020-08-18
JP2020138106 2020-08-18
JP2021-075272 2021-04-27
JP2021075272 2021-04-27
PCT/JP2021/030058 WO2022039165A1 (ja) 2020-08-18 2021-08-17 多能性幹細胞の外胚葉、中胚葉及び内胚葉系細胞への分化誘導方法

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EP (1) EP4180515A4 (https=)
JP (1) JPWO2022039165A1 (https=)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116731967A (zh) * 2023-08-16 2023-09-12 南京大学 从多能干细胞通过诱导分化制备巨噬细胞的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020062009A (ja) * 2018-10-16 2020-04-23 東ソー株式会社 細胞培養基材、細胞培養基材の製造方法、及びスフェロイドの製造方法
WO2022230734A1 (ja) * 2021-04-27 2022-11-03 東ソー株式会社 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット
JP7829851B2 (ja) * 2021-10-14 2026-03-16 大日本印刷株式会社 組織の製造方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011118211A1 (ja) * 2010-03-23 2011-09-29 株式会社クラレ 多能性哺乳細胞を分化させる培養方法
JP2015015943A (ja) * 2013-06-10 2015-01-29 大日本印刷株式会社 人工多能性幹細胞の分化誘導方法
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754653A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成内胚层祖细胞的无血清诱导培养基及诱导方法
JP2017093387A (ja) * 2015-11-26 2017-06-01 学校法人 創価大学 胚様体を外胚葉へと分化誘導する方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3660601B2 (ja) 2001-03-30 2005-06-15 独立行政法人科学技術振興機構 胚性幹細胞からの神経幹細胞、運動ニューロン及びｇａｂａ作動性ニューロンの製造法
WO2008001938A1 (en) 2006-06-27 2008-01-03 Shiseido Company, Ltd. Cell cluster comprising plural kinds of cells derived from soma with ability to form primitive organ-like structure
CA2669939A1 (en) * 2006-10-12 2008-05-22 Massachusetts Institute Of Technology Multi-well micropatterning by ablation
WO2011026221A1 (en) * 2009-09-01 2011-03-10 Mcmaster University Methods of culturing embryoid bodies
JP6151097B2 (ja) * 2013-06-10 2017-06-21 大日本印刷株式会社 腸構造体を分化誘導する方法
FR3024462A1 (fr) * 2014-07-29 2016-02-05 Univ Pierre Et Marie Curie Paris 6 Procede de production in vitro de progeniteurs adipocytaires et d'adipocytes
JP6979687B2 (ja) * 2015-12-29 2021-12-15 アイ ピース インク 幹細胞の凝集塊の集団の調製方法
HK1256807A1 (zh) 2016-01-14 2019-10-04 R·阿南德 神经类器官组合物及使用方法
JP2017209081A (ja) * 2016-05-27 2017-11-30 クアーズテック株式会社 細胞培養担体および細胞培養モジュール
JP2020043764A (ja) 2016-12-28 2020-03-26 Ａｇｃテクノグラス株式会社 細胞培養基材及びその製造方法
JP7008294B2 (ja) 2017-06-13 2022-02-10 大日本印刷株式会社 腸オルガノイド及びその作製方法
JP7035616B2 (ja) * 2018-02-26 2022-03-15 日本ゼオン株式会社 人工多能性幹細胞の分化誘導方法
JP2021521792A (ja) * 2018-04-20 2021-08-30 フジフィルム セルラー ダイナミクス，インコーポレイテッド 眼球細胞の分化方法及びその使用
JP2020062009A (ja) 2018-10-16 2020-04-23 東ソー株式会社 細胞培養基材、細胞培養基材の製造方法、及びスフェロイドの製造方法
US20220106551A1 (en) * 2019-01-30 2022-04-07 Dai Nippon Printing Co., Ltd. Cell culture substrate
CN113692442A (zh) * 2019-04-17 2021-11-23 学校法人庆应义塾 诱导多能干细胞的制造方法以及试剂盒
WO2020218579A1 (ja) * 2019-04-26 2020-10-29 国立大学法人京都大学 分化誘導のために馴化された多能性幹細胞の作製方法
JP2021158960A (ja) * 2020-03-31 2021-10-11 東ソー株式会社 多能性幹細胞から外胚葉系細胞を分化誘導する方法及び製造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011118211A1 (ja) * 2010-03-23 2011-09-29 株式会社クラレ 多能性哺乳細胞を分化させる培養方法
JP2015015943A (ja) * 2013-06-10 2015-01-29 大日本印刷株式会社 人工多能性幹細胞の分化誘導方法
JP2017093387A (ja) * 2015-11-26 2017-06-01 学校法人 創価大学 胚様体を外胚葉へと分化誘導する方法
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754653A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成内胚层祖细胞的无血清诱导培养基及诱导方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
C´ELINE LIU BAUWENS ET AL.: "Control of Human Embryonic Stem Cell Colony and Aggregate Size Heterogeneity Influences Differentiation Trajectories", STEMCELLS, vol. 26, 31 December 2008 (2008-12-31), pages 2300 *
吴木潮等: "胰岛β细胞 基础与临床", vol. 1, 30 June 2017, 广东科技出版社, pages: 340 - 341 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116731967A (zh) * 2023-08-16 2023-09-12 南京大学 从多能干细胞通过诱导分化制备巨噬细胞的方法
CN116731967B (zh) * 2023-08-16 2023-11-17 南京大学 从多能干细胞通过诱导分化制备巨噬细胞的方法

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WO2022039165A1 (ja) 2022-02-24

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